Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| [triatoma dimidiata in colombia: distribution, ecology and epidemiological importance]. | triatoma dimidiata is an important vector of chagas disease in central america and countries of northern south america. in colombia, it has a wide geographical distribution with reported presence in 14 departments in the andean, caribbean, eastern plains and upper magdalena regions, where it occupies different natural and artificial ecotopes. the species is considered a secondary vector in the transmission of trypanosoma cruzi. its presence in wild, peridomestic and intradomiciliary habitats in ... | 2017 | 28527292 |
| [possible oral transmission of chagas disease among hydrocarbons sector workers in casanare, colombia, 2014]. | trypanosoma cruzi, the etiological agent for chagas disease, can be transmitted by oral intake of contaminated food or drinks. during epidemiological week 14 of 2014, two cases of acute chagas disease were notified among hydrocarbons sector workers in paz de ariporo, casanare. | 2017 | 28527286 |
| [risk of transmission of chagas disease by intrusion of triatomines and wild mammals in bucaramanga, santander, colombia]. | notice of triatomines in dwellings of some neighborhoods in bucaramanga motivated the realization of this study.objetive: to evaluate the intrusion of triatomines and mammals, as well as some risk factors in urban dwellings. | 2017 | 28527250 |
| [diversity of triatominae (hemiptera: reduviidae) in santander, colombia: epidemiological implications]. | domestic and wild triatomines in the department of santander have an epidemiological impact, as recently they have been linked to outbreaks of acute chagas disease. the analysis of their diversity and temporal variation contributes to the understanding of their biology and ecology in one of the most endemic areas of the country. | 2017 | 28527247 |
| myenteric neuroprotective role of aspirin in acute and chronic experimental infections with trypanosoma cruzi. | experimental and clinical studies have shown that myenteric neuron cell death during infection with trypanosoma cruzi mainly occurs in the esophagus and colon, resulting in megaesophagus and megacolon, respectively. evidence suggests that the cyclooxygenase enzyme (cox) is involved in the t. cruzi invasion process. the use of low-dose aspirin (asa), a cox-1/cox-2 inhibitor, has been shown to reduce infection with t. cruzi. therefore, in this study, we evaluated the effects of treatment with low- ... | 2017 | 28524628 |
| molecular docking studies applied to a dataset of cruzain inhibitors. | chagas' disease is one of the main causes of heart failure in developing countries. the disadvantages of current therapy include the undesirable side-effects, resistance, and therapeutic adhesion. the development of new efficient and safe drugs is, therefore, an issue of extreme importance. | 2017 | 28523999 |
| trypanosoma cruzi over the ocean: insular zones of chile with presence of infected vector mepraia species. | chagas disease is one of the main zoonosis mediated by vectors in america. the etiologic agent trypanosoma cruzi infects different mammals and is transmitted principally by the subfamily triatominae. mepraia is a genus endemic to chile, responsible for transmitting t. cruzi in the sylvatic cycle. mepraia includes three species: m. gajardoi and m. parapatrica inhabit coastal areas, while m. spinolai inhabits coastal and interior valleys. previous studies reported the occurrence of mepraia in pan ... | 2017 | 28522273 |
| leishmanicidal and trypanocidal activity of metal complexes with 1,2,4-triazolo[1,5-a]pyrimidines: insights on their therapeutic potential against leishmaniasis and chagas disease. | since their first synthesis back in the early 20th century, 1,2,4-triazolo[1,5-a]pyrimidines have aroused increasing interest in very diverse areas ranging from chemotherapy to agriculture or even photography. their similarity to purines confers a potential bioactivity and this feature has been wisely exploited for therapeutic use, including antifungal, antipyretic, analgesic, antiinflammatory, antitumoral and antiparasitic properties. in this review, we focus on the compounds that these nitroge ... | 2017 | 28521698 |
| rhodium-catalyzed c-h bond activation for the synthesis of quinonoid compounds: significant anti-trypanosoma cruzi activities and electrochemical studies of functionalized quinones. | thirty four halogen and selenium-containing quinones, synthesized by rhodium-catalyzed c-h bond activation and palladium-catalyzed cross-coupling reactions, were evaluated against bloodstream trypomastigotes of t. cruzi. we have identified fifteen compounds with ic50/24 h values of less than 2 μm. electrochemical studies on a-ring functionalized naphthoquinones were also performed aiming to correlate redox properties with trypanocidal activity. for instance, (e)-5-styryl-1,4-naphthoquinone 59 an ... | 2017 | 28521262 |
| sex-dependent infection causes nonadditive effects on kissing bug fecundity. | the influence of parasites on host reproduction has been widely studied in natural and experimental conditions. most studies, however, have evaluated the parasite impact on female hosts only, neglecting the contribution of males for host reproduction. this omission is unfortunate as sex-dependent infection may have important implications for host-parasite associations. here, we evaluate for the first time the independent and nonindependent effects of gender infection on host reproductive success ... | 2017 | 28515890 |
| trypanosoma cruzi in free-ranging mammalian populations in south texas. | chagas disease, also known as american trypanosomiasis, is caused by the flagellate protozoan trypanosoma cruzi. it is a significant health concern in south and central america, where millions of people are infected or at risk of infection, and is an emerging health concern in the united states. the occurrence of chagas disease in natural environments is supported by mammal host species, but those primary species may vary based on geographic location. in south texas, the primary host species for ... | 2017 | 28513328 |
| a case of chagas cardiomyopathy following infection in south central texas. | between 5 and 8 million people globally are infected with trypanosoma cruzi, the causative parasitic agent of chagas disease. the vast majority of incident infections originate in pockets of latin america where domestic vector-borne transmission cycles are more common. since 1955, when the first locally-acquired case was reported, fewer than 30 autochthonous cases have been documented in the united states. we describe the case of an 18-year-old us air force trainee, a native texan with no travel ... | 2017 | 28511274 |
| molecular modeling and structure-activity relationships for a series of benzimidazole derivatives as cruzain inhibitors. | chagas disease is endemic in latin america and no effective treatment is available. efforts in drug research have focused on several enzymes from trypanosoma cruzi, among which cruzain is a validated pharmacological target. | 2017 | 28509592 |
| fish oil supplementation benefits the murine host during the acute phase of a parasitic infection from trypanosoma cruzi. | long-chain omega-3 polyunsaturated fatty acids (lc n-3 pufa) are known to modulate a variety of immune cell functions. on occasion, this has led to diminished host resistance to certain viral and bacterial infections. little is known about the impact of n-3 pufa on host resistance to parasitic infection, however, based on results from a small study conducted more than two decades ago, we hypothesized that providing mice lc n-3 pufa will diminish host resistance to trypanosoma cruzi, the parasiti ... | 2017 | 28506517 |
| chagas disease in europe: a review for the internist in the globalized world. | chagas disease (cd) or american trypanosomiasis identified in 1909 by carlos chagas, has become over the last 40years a global health concern due to the huge migration flows from latin america to europe, united states, canada and japan. in europe, most migrants from cd-endemic areas are concentrated in spain, italy, france, united kingdom and switzerland. pooled seroprevalence studies conducted in europe show an overall 4.2% prevalence, with the highest infection rates observed among individuals ... | 2017 | 28502864 |
| the prevalence of chagas disease among latin american immigrants with pacemakers in los angeles, california. | abstractchagas disease (cd), with associated conduction abnormalities, is a common indication for pacemaker implantation in latin america. the prevalence of cd in latin american immigrants with pacemakers residing in the united states has never been studied. this single-center cross-sectional study included pacemaker patients who were aged 18 years or more with a previous residence in latin america for at least 6 months. patients with an implantable cardioverter-defibrillator, cardiac resynchron ... | 2017 | 28500812 |
| identification of novel mammalian hosts and brazilian biome geographic distribution of trypanosoma cruzi tciii and tciv. | trypanosoma cruzi is a parasitic protozoan responsible for chagas disease. seven different discrete typing units (dtus) of t. cruzi are currently identified in nature: tci-tcvi, and tcbat whose distribution patterns in nature, hosts/reservoirs and eco-epidemiological importance are still little known. here, we present novel data on the geographic distribution and diversity of mammalian hosts and vectors of t. cruzi dtus tciii and tciv. in this study, we analyzed 61 t. cruzi isolates obtained fro ... | 2017 | 28499908 |
| novel imidazo[4,5-c][1,2,6]thiadiazine 2,2-dioxides as antiproliferative trypanosoma cruzi drugs: computational screening from neural network, synthesis and in vivo biological properties. | a new family of imidazo[4,5-c][1,2,6]thiadiazine 2,2-dioxide with antiproliferative trypanosoma cruzi properties was identified from a neural network model published by our group. the synthesis and evaluation of this new class of trypanocidal agents are described. these compounds inhibit the growth of trypanosoma cruzi, comparable with benznidazole or nifurtimox. in vitro assays were performed to study their effects on the growth of the epimastigote form of the tulahuen 2 strain, as well as the ... | 2017 | 28499168 |
| trypanosoma cruzi i: towards the need of genetic subdivision?, part ii. | chagas disease is a complex zoonosis caused by the kinetoplastid parasite trypanosoma cruzi. this protozoan exhibits remarkable genetic diversity evinced in at least six discrete typing units (dtus) with the foreseen emergence of a genotype associated to bats (tcbat). t. cruzi i is the dtu with the broadest geographical distribution and associated to severe cardiomyopathies. in 2011, we published a review questioning the need for genetic subdivision within tci. however, after six years of intens ... | 2017 | 28495405 |
| access to chagas disease treatment in non-endemic countries: the case of spain. | 2017 | 28495262 | |
| the neuroprotective effects of phenolic acids: molecular mechanism of action. | the neuroprotective role of phenolic acids from food has previously been reported by many authors. in this review, the role of phenolic acids in ameliorating depression, ischemia/reperfusion injury, neuroinflammation, apoptosis, glutamate-induced toxicity, epilepsy, imbalance after traumatic brain injury, hyperinsulinemia-induced memory impairment, hearing and vision disturbances, parkinson's disease, huntington's disease, anti-amyotrophic lateral sclerosis, chagas disease and other less distrib ... | 2017 | 28489058 |
| different roles of mitochondrial calcium uniporter complex subunits in growth and infectivity of trypanosoma cruzi. | trypanosoma cruzi is the agent of chagas disease, and the finding that this parasite possesses a mitochondrial calcium uniporter (tcmcu) with characteristics similar to that of mammalian mitochondria was fundamental for the discovery of the molecular nature of mcu in eukaryotes. we report here that ablation of tcmcu, or its paralog tcmcub, by clustered regularly interspaced short palindromic repeat (crispr)/cas9 led to a marked decrease in mitochondrial ca(2+) uptake without affecting the membra ... | 2017 | 28487431 |
| epidemiological modeling of trypanosoma cruzi: low stercorarian transmission and failure of host adaptive immunity explain the frequency of mixed infections in humans. | people living in areas with active vector-borne transmission of chagas disease have multiple contacts with its causative agent, trypanosoma cruzi. reinfections by t. cruzi are possible at least in animal models leading to lower or even hardly detectable parasitaemia. in humans, although reinfections are thought to have major public health implications by increasing the risk of chronic manifestations of the disease, there is little quantitative knowledge about their frequency and the timing of pa ... | 2017 | 28481887 |
| quantum mechanics/molecular mechanics studies of the mechanism of cysteine protease inhibition by peptidyl-2,3-epoxyketones. | cysteine proteases are the most abundant proteases in parasitic protozoa and they are essential enzymes to the life cycle of several of them, thus becoming attractive therapeutic targets for the development of new inhibitors. in this paper, a computational study of the inhibition mechanism of cysteine protease by dipeptidyl-2,3-epoxyketone cbz-phe-hph-(s), a recently proposed inhibitor, has been carried out by means of molecular dynamics (md) simulations with hybrid qm/mm potentials. the compute ... | 2017 | 28480929 |
| seroprevalence of trypanosoma cruzi (tc) and risk factors in colima, mexico. | the present study was conducted to estimate the incidence of seropositivity to anti-trypanosoma cruzi antibodies and analyze potential risk factors in colima, on the western coast of mexico. | 2017 | 28474704 |
| the complement system: a prey of trypanosoma cruzi. | trypanosoma cruzi is a protozoan parasite known to cause chagas disease (cd), a neglected sickness that affects around 6-8 million people worldwide. originally, cd was mainly found in latin america but more recently, it has been spread to countries in north america, asia, and europe due the international migration from endemic areas. thus, at present cd represents an important concern of global public health. most of individuals that are infected by t. cruzi may remain in asymptomatic form all l ... | 2017 | 28473804 |
| regarding perspective piece from july 2016 "what do we know about chagas disease in the united states?" | 2017 | 28471741 | |
| genetic polymorphisms of il17 and chagas disease in the south and southeast of brazil. | the aim of this study was to investigate possible associations between genetic polymorphisms of il17a g197a (rs2275913) and il17f t7488c (rs763780) with chagas disease (cd) and/or the severity of left ventricular systolic dysfunction (lvsd) in patients with chronic chagas cardiomyopathy (ccc). the study with 260 patients and 150 controls was conducted in the south and southeast regions of brazil. the genotyping was performed by pcr-rflp. the a allele and a/a genotype of il17a were significantly ... | 2017 | 28470012 |
| developmental roles of tyrosine metabolism enzymes in the blood-sucking insect rhodnius prolixus. | the phenylalanine/tyrosine degradation pathway is frequently described as a catabolic pathway that funnels aromatic amino acids into citric acid cycle intermediates. previously, we demonstrated that the accumulation of tyrosine generated during the hydrolysis of blood meal proteins in rhodnius prolixus is potentially toxic, a harmful outcome that is prevented by the action of the first two enzymes in the tyrosine degradation pathway. in this work, we further evaluated the relevance of all other ... | 2017 | 28469016 |
| supergiant fecaloma as manifestation of chronic constipation. | fecaloma is common in patients with damage to the autonomic nervous system in the large bowel associated with chagas disease (inflammatory and neoplastic) or hirschprung's disease, in psychiatric patients and, more commonly, in elderly patients suffering with chronic constipation. symptoms of fecaloma are usually nonspecific. clinical examination can give the appearance of an abdominal tumor. most cases of fecaloma are treated conservatively with digital evacuation and enemas. in severe and unre ... | 2017 | 28460206 |
| identification of specific inhibitors of trypanosoma cruzi malic enzyme isoforms by target-based hts. | trypanosoma cruzi is the causative agent of chagas disease. the lack of an efficient and safe treatment supports the research into novel metabolic targets, with the malic enzyme (me) representing one such potential candidate. t. cruzi expresses a cytosolic (tcmec) and a mitochondrial (tcmem) me isoform, with these activities functioning to generate nadph, a key source of reducing equivalents that drives a range of anabolic and protective processes. to identify specific inhibitors that target tcm ... | 2017 | 28459632 |
| comparison of four pcr methods for efficient detection of trypanosoma cruzi in routine diagnostics. | due to increased migration, chagas disease has become an international health problem. reliable diagnosis of chronically infected people is crucial for prevention of non-vectorial transmission as well as treatment. this study compared four distinct pcr methods for detection of trypanosoma cruzi dna for the use in well-equipped routine diagnostic laboratories. dna was extracted of t. cruzi-positive and negative patients' blood samples and cultured t. cruzi, t. rangeli as well as leishmania spp. o ... | 2017 | 28456430 |
| phenotypic variability of the amazonian species rhodnius brethesi (hemiptera: reduviidae). | the wild species rhodnius brethesi (matta, 1919) (hemiptera: reduviidae) is found in areas of piassabais in microregion of rio negro, amazonas, brazil. its geographical distribution overlaps the areas of leopoldinia piassaba palm. in areas where palm trees are found, transmission cycle of trypanosoma cruzi is related to the extractive activity of the palm fiber, exposing workers to wild vector transmission of this parasite. the close association with the palm tree l. piassaba suggests that this ... | 2017 | 28449112 |
| chagas disease: national survey of seroprevalence in children under five years of age conducted in 2008. | since the early 1990s, programs to control chagas disease in south america have focused on eradicating domiciliary triatoma infestans, the main vector. seroprevalence studies of the chagasic infection are included as part of the vector control programs; they are essential to assess the impact of vector control measures and to monitor the prevention of vector transmission. | 2017 | 28443980 |
| proteomic and transcriptomic analysis of saliva components from the hematophagous reduviid triatoma pallidipennis. | species belonging to the triatominae subfamily are commonly associated with chagas disease, as they are potential vectors of the parasite trypanosoma cruzi. however, their saliva contains a cocktail of diverse anti-hemostatic proteins that prevent blood coagulation, vasodilation and platelet aggregation of blood; components with indisputable therapeutic potential. we performed a transcriptomic and proteomic analyses of salivary glands and protein spots from 2de gels of milked saliva, respectivel ... | 2017 | 28442446 |
| automated identification of insect vectors of chagas disease in brazil and mexico: the virtual vector lab. | identification of arthropods important in disease transmission is a crucial, yet difficult, task that can demand considerable training and experience. an important case in point is that of the 150+ species of triatominae, vectors of trypanosoma cruzi, causative agent of chagas disease across the americas. we present a fully automated system that is able to identify triatomine bugs from mexico and brazil with an accuracy consistently above 80%, and with considerable potential for further improvem ... | 2017 | 28439451 |
| phenotypic evaluation and in silico admet properties of novel arylimidamides in acute mouse models of trypanosoma cruzi infection. | arylimidamides (aias), previously termed as reversed amidines, present a broad spectrum of activity against intracellular microorganisms. in the present study, three novel aias were evaluated in a mouse model of trypanosoma cruzi infection, which is the causative agent of chagas disease. the bis-aias db1957, db1959 and db1890 were chosen based on a previous screening of their scaffolds that revealed a very promising trypanocidal effect at nanomolar range against both the bloodstream trypomastigo ... | 2017 | 28435221 |
| modeling chagas disease in chile: from vector to congenital transmission. | chagaś disease is a human health problem in latin america. it is highly prevalent in northern chile between the arica-parinacota and coquimbo regions, with reported incidence of 3-11/100000 inhabitants and mortality of 0.3-0.4/100000. the interruption of vector transmission was reported in 1999 by means of the elimination of the primary vector, triatoma infestans, from human dwellings, thus the epidemiologic dynamics of this disease should be modified. here we model the dynamics of chagaś dise ... | 2017 | 28435013 |
| rhodnius prolixus: from classical physiology to modern developmental biology. | the hemiptera rhodnius prolixus is a blood-feeding insect and a primary vector of trypanosoma cruzi, the etiological agent of the chagas disease. over the past century, rhodnius has been the subject of intense investigations, which have contributed to unveil important aspects of metabolism and physiology in insects. recent technological innovations are helping dissect the genetic and molecular underpinnings of rhodnius embryogenesis and organogenesis, thus fostering the use of this important spe ... | 2017 | 28432816 |
| challenges in the management of chagas disease in latin-american migrants in europe. | chagas disease is endemic in latin america. due to migration the infection has crossed borders and it is estimated that 68,000-120,000 people with chagas disease are currently living in europe and 30% of them may develop visceral involvement. however, up to 90% of chagas disease cases in europe remain undiagnosed. the challenges which have to be overcome in chagas disease in non-endemic countries are focused on related downing barriers to health care access, and related to screening, diagnostic ... | 2017 | 28428122 |
| ten years of chagas disease research: looking back to achievements, looking ahead to challenges. | 2017 | 28426735 | |
| the effectiveness of riboflavin and ultraviolet light pathogen reduction technology in eliminating trypanosoma cruzi from leukoreduced whole blood. | the parasitic chagas disease is caused by the protozoan trypanosoma cruzi, which is mainly transmitted by insect vectors. other infection routes, both in endemic and in nonendemic areas, include organ and marrow transplantation, congenital transmission, and blood transfusion. asymptomatic chronic chagasic individuals may have a low and transient parasitemia in peripheral blood and, consequently, they can unknowingly transmit the disease via blood transfusion. riboflavin and ultraviolet (uv) ligh ... | 2017 | 28419477 |
| autochthonous chagas disease in the southern united states: a case report of suspected residential and military exposures. | chagas disease is a parasitic infection that can result in a progressive dilated cardiomyopathy. here, we present the epidemiologic details of a suspected locally acquired transmission case originating from the southern united states. this is the first published report of chagas disease in a young, healthy united states veteran with repeat triatomine exposures in arizona. military personnel and arizona residents should be aware of their chagas disease transmission risks. | 2017 | 28418113 |
| trypanosoma cruzi reservoir-triatomine vector co-occurrence networks reveal meta-community effects by synanthropic mammals on geographic dispersal. | contemporary patterns of land use and global climate change are modifying regional pools of parasite host species. the impact of host community changes on human disease risk, however, is difficult to assess due to a lack of information about zoonotic parasite host assemblages. we have used a recently developed method to infer parasite-host interactions for chagas disease (cd) from vector-host co-occurrence networks. vector-host networks were constructed to analyze topological characteristics of ... | 2017 | 28413725 |
| enhancement of dissolution rate through eutectic mixture and solid solution of posaconazole and benznidazole. | benznidazole (bnz), the only commercialized antichagasic drug, and the antifungal compound posaconazole (pcz) have shown synergistic action in the therapy of chagas disease, however both active pharmaceutical ingredients (apis) exhibit low aqueous solubility potentially limiting their bioavailability and therapeutic efficacy. in this paper, we report for the first time the formation of a eutectic mixture as well as an amorphous solid solution of pcz and bnz (at the same characteristic ratio of 8 ... | 2017 | 28412452 |
| [seroprevalence and vertical transmission of chagas disease in a cohort of latin-american pregnant women in a tertiary hospital in madrid]. | chagas disease, caused by trypanosoma cruzi (t. cruzi), is endemic in latin-america and is emerging in spain due to immigration. the vertical transmission rate is around 5%. a routine prenatal screening with serology of all pregnant women from endemic areas is recommended to identify infected newborns, allowing early treatment and cure. | 2017 | 28411096 |
| triatoma vitticeps subcomplex (hemiptera, reduviidae, triatominae): a new grouping of chagas disease vectors from south america. | triatomines have been grouped into complexes and subcomplexes based largely on morphological and geographical distribution. although these groupings are not formally recognised as taxonomic ranks, they are likely monophyletic. however, recent studies have demonstrated that some subcomplexes from south america did not form monophyletic groups, and reorganisations have been suggested. one suggested reorganisation is to exclude triatoma vitticeps, t. melanocephala, and t. tibiamaculata from the t. ... | 2017 | 28407782 |
| differential transcriptome analysis supports rhodnius montenegrensis and rhodnius robustus (hemiptera, reduviidae, triatominae) as distinct species. | chagas disease is one of the main parasitic diseases found in latin america and it is estimated that between six and seven million people are infected worldwide. its etiologic agent, the protozoan trypanosoma cruzi, is transmitted by triatomines, some of which from the genus rhodnius. twenty species are currently recognized in this genus, including some closely related species with low levels of morphological differentiation, such as rhodnius montenegrensis and rhodnius robustus. in order to inv ... | 2017 | 28406967 |
| evidence of neurofibromatosis type 1 in a multi-morbid inca child mummy: a paleoradiological investigation using computed tomography. | in this study, an inca bundle was examined using computed tomography (ct). the primary aim was to determine the preservation status of bony and soft tissues, the sex, the age at the time of death, possible indicators for disease or even the cause of death, as well as the kind of mummification. a secondary aim was to obtain a brief overview of the wrapping in order to gain additional information on the cultural background. | 2017 | 28403237 |
| the botanical monoterpenes linalool and eugenol flush-out nymphs of triatoma infestans (hemiptera: reduviidae). | monoterpenes are the main components of essential oils. some members of this chemical family present insecticidal activity. triatoma infestans (klug) is the main vector of chagas disease in argentina, bolivia, paraguay, and perú. the objective of this work was to evaluate the effect of six monoterpenes (1,8-cineole, eugenol, linalool, menthol, α-terpineol, and thymol) on the locomotor and flushing out activity of t. infestans. a video tracking technique was used to evaluate the locomotor activit ... | 2017 | 28399279 |
| biology of triatoma sherlocki (hemiptera: reduviidae) under laboratory conditions: biological cycle and resistance to starvation. | triatoma sherlocki papa, jurberg, carcavallo, cerqueira & barata was described in 2002, based on specimens caught in the wild in the municipality of gentio do ouro, bahia, brazil. in 2009, nymphs and adults were detected inside homes and sylvatic specimens were positive for trypanosoma cruzi (chagas). no information on the bionomics of t. sherlocki exists, although such data are considered essential to estimate its vector and colonization potential in domestic environments. herein, the biologica ... | 2017 | 28399226 |
| house reinfestation with triatoma infestans (hemiptera: reduviidae) after community-wide spraying with insecticides in the argentine chaco: a multifactorial process. | we investigated the dynamics and underlying causes of house (re)infestation with triatoma infestans (klug 1834) after a community-wide residual spraying with pyrethroids in a well-defined rural section of pampa del indio municipality (northeastern argentina) over a 4-yr period. house infestation was assessed by timed manual searches, during insecticide applications, and by opportunistic householders' bug collections. all reinfested houses were selectively re-sprayed with insecticides. the reside ... | 2017 | 28399199 |
| the contemporary distribution of trypanosoma cruzi infection in humans, alternative hosts and vectors. | chagas is a potentially fatal chronic disease affecting large numbers of people across the americas and exported throughout the world through human population movement. it is caused by the trypanosoma cruzi parasite, which is transmitted by triatomine vectors to humans and a wide range of alternative host species. the database described here was compiled to allow the risk of vectorial transmission to humans to be mapped using geospatial models. the database collates all available records, publis ... | 2017 | 28398292 |
| [treatment and follow up of chagas disease in immunocompromised hosts]. | 2017 | 28394984 | |
| chagas disease immunogenetics: elusive markers of disease progression. | chagas disease (cd) is caused by a parasitic infection. the disease usually occurs after decades of the primary infection and can involve the myocardium or the digestive system. of note, around 30% of t. cruzi infected patients develop cd while the other 70% may remain asymptomatic for their entire life. cd is usually observed as familial clustered phenomena. moreover, individuals with chronic chagas heart disease (cchd) usually present a strong, deregulated immune response, which strongly sugge ... | 2017 | 28388241 |
| evolution of the endomembrane systems of trypanosomatids - conservation and specialisation. | parasite surfaces support multiple functions required for survival within their hosts, and maintenance and functionality of the surface depends on membrane trafficking. to understand the evolutionary history of trypanosomatid trafficking, where multiple lifestyles and mechanisms of host interactions are known, we examined protein families central to defining intracellular compartments and mediating transport, namely rabs, snares and rabgaps, across all available euglenozoa genomes. bodonids poss ... | 2017 | 28386020 |
| high-resolution manometry findings in patients with achalasia and massive dilated megaesophagus. | achalasia may present in a non-advanced or an advanced (end stage) stage based on the degree of esophageal dilatation. manometric parameters and esophageal caliber may be prognostic for the outcome of treatment. the correlation between manometry and disease stage has not been yet fully studied. this study aims to describe high-resolution manometry findings in patients with achalasia and massive dilated megaesophagus. eighteen patients (mean age 61 years, 55% females) with achalasia and massive d ... | 2017 | 28375440 |
| inhibitors of pex14 disrupt protein import into glycosomes and kill trypanosoma parasites. | the parasitic protists of the trypanosoma genus infect humans and domestic mammals, causing severe mortality and huge economic losses. the most threatening trypanosomiasis is chagas disease, affecting up to 12 million people in the americas. we report a way to selectively kill trypanosoma by blocking glycosomal/peroxisomal import that depends on the pex14-pex5 protein-protein interaction. we developed small molecules that efficiently disrupt the pex14-pex5 interaction. this results in mislocaliz ... | 2017 | 28360328 |
| epithelial cell types and their proposed roles in maintaining the mucosal barrier in human chagasic-megacolonic mucosa. | patients suffering from chagasic megacolon must have an intact mucosal barrier as they survive this chronic disease for decades. a key structure of the mucosal barrier are epithelial cells. vasoactive-intestinal-peptide (vip)-positive nerve fibres are involved in influencing, e.g., epithelial cell proliferation, mucus secretion (e.g., mucin 2 and trefoil factor 3 of goblet cells) and inflammation or autoimmunity, all putative and/or known factors altered in chagasic megacolon. we analyzed qualit ... | 2017 | 28357579 |
| chagas disease in the united states: out of the shadows. | 2017 | 28329373 | |
| prevalence of chagas disease in the latin american-born population of los angeles. | according to an estimate from the centers for disease control and prevention (cdc), chagas disease (cd) may affect 1.31% of latin american immigrants in the united states, with >300 000 cases. however, there is a lack of real-world data to support this estimate. little is known about the actual prevalence of this neglected tropical disease in the united states, and the bulk of those infected are undiagnosed. | 2017 | 28329123 |
| adverse systemic reaction to benznidazole. | benznidazole, drug of choice for chagas disease (cd), has been associated with a high incidence of adverse reactions that can become serious, necessitating discontinuation of the drug. we describe the case of a bolivian patient living in spain for 9 years, who, following treatment with benznidazole for cd in indeterminate chronic phase, presented with fever, skin lesions, digestive symptoms, general malaise, and laboratory abnormalities. after the discontinuation of benznidazole and, the intake ... | 2017 | 28327820 |
| the development of panstrongylus herreri under fluctuating environmental conditions. | panstrongylus herreri is a main chagas disease vector, and its success as a vector stems from its ability to establish domiciliated colonies; we aimed to explore its biology and reproduction. | 2017 | 28327814 |
| autoantibodies with beta-adrenergic activity from chronic chagasic patients induce cardiac arrhythmias and early afterdepolarization in a drug-induced lqt2 rabbit hearts. | cardiac arrhythmias are one of the main causes of death in chcp and other dilated cardiomyopathies. previous studies demonstrated that ventricular arrhythmias are associated with the presence of autoantibodies with beta-adrenergic activity, ab-β. | 2017 | 28320606 |
| pediatric enteric neuropathies: diagnosis and current management. | neurointestinal diseases are increasingly recognized as causes of significant gastrointestinal morbidity in children. this review highlights the most common pediatric enteric neuropathies and their diagnosis and management, emphasizing insights and discoveries from the most recent literature available. | 2017 | 28319561 |
| 18-des-hydroxy cytochalasin: an antiparasitic compound of diaporthe phaseolorum-92c, an endophytic fungus isolated from combretum lanceolatum pohl ex eichler. | chemical investigation of the ethyl acetate extract from the endophytic fungus diaporthe phaseolorum-92c (92c) isolated from the roots of combretum lanceolatum led to the isolation of 18-des-hydroxy cytochalasin h (compound 1). the trypanocidal and schistosomicidal activity and cytotoxicity of the extract from 92c were evaluated. the schistosomicidal, leishmanicidal, antimicrobial, and antioxidant actions, as well as the antitumor activity against the breast cancer cells mda-mb-231 and mcf-7, an ... | 2017 | 28497228 |
| tropical diseases of the myocardium: a review. | cardiovascular diseases are widely distributed throughout the world. human parasitic infections are ubiquitous. tropical parasites are increasingly recognized as causes of cardiovascular diseases. in this review, we address the most frequently reported parasites that directly infect the myocardium, including trypanosoma cruzi, the protozoal causative agent of american trypanosomiasis (chagas disease), and taenia solium, the cestode causative agent of taeniasis and cysticercosis. we also discuss ... | 2017 | 28435310 |
| tandem affinity purification of exosome and replication factor c complexes from the non-human infectious kinetoplastid parasite crithidia fasciculata. | kinetoplastid parasites are responsible for a range of diseases with significant global impact. trypanosoma brucei and trypanosoma cruzi cause human african trypanosomiasis and chagas disease, respectively, while various leishmania species are responsible for cutaneous, mucocutaneous and visceral leishmaniasis. understanding the biology of these organisms is key for effective diagnosis, prophylaxis and treatment. the insect parasite crithidia fasciculata offers a safe and low-cost alternative fo ... | 2017 | 28844893 |
| lipidomics and anti-trypanosomatid chemotherapy. | trypanosomatids such as leishmania, trypanosoma brucei and trypanosoma cruzi belong to the order kinetoplastida and are the source of many significant human and animal diseases. current treatment is unsatisfactory and is compromised by the rising appearance of drug resistant parasites. novel and more effective chemotherapeutics are urgently needed to treat and prevent these devastating diseases, which relies on the identification of essential, parasite specific targets that are absent in the hos ... | 2017 | 28766182 |
| new class of antitrypanosomal agents based on imidazopyridines. | the present work describes the synthesis of 22 new imidazopyridine analogues arising from medicinal chemistry optimization at different sites on the molecule. seven and 12 compounds exhibited an in vitro ec50 ≤ 1 μm against trypanosoma cruzi (t. cruzi) and trypanosoma brucei (t. brucei) parasites, respectively. based on promising results of in vitro activity (ec50 < 100 nm), cytotoxicity, metabolic stability, protein binding, and pharmacokinetics (pk) properties, compound 20 was selected as a ca ... | 2017 | 28740614 |
| inhibitors of glycosomal protein import provide new leads against trypanosomiasis. | vector-borne trypanosomatid parasite infections in tropical and sub-tropical countries constitute a major threat to humans and livestock. trypanosoma brucei parasites are transmitted by tsetse fly and lead to african sleeping sickness in humans and nagana in cattle. in latin american countries, trypanosoma cruzi infections spread by triatomine kissing bugs lead to chagas disease. various species of leishmania transmitted to humans by phlebotomine sandflies manifest in a spectrum of diseases term ... | 2017 | 28706938 |
| subcellular localization of glycolytic enzymes and characterization of intermediary metabolism of trypanosoma rangeli. | trypanosoma rangeli is a hemoflagellate protist that infects wild and domestic mammals as well as humans in central and south america. although this parasite is not pathogenic for human, it is being studied because it shares with trypanosoma cruzi, the etiological agent of chagas' disease, biological characteristics, geographic distribution, vectors and vertebrate hosts. several metabolic studies have been performed with t. cruzi epimastigotes, however little is known about the metabolism of t. ... | 2017 | 28645481 |
| use of zymography in trypanosomiasis studies. | zymography assay is a semiquantitative technique, very sensitive, and commonly used to determine metalloproteinase levels in different types of biological samples, including tissues, cells, and extracts of protein. samples containing metalloproteinases are loaded onto a polyacrylamide gel containing sodium dodecyl sulphate (sds) and a specific substrate (gelatin, casein, collagen, etc.). then proteins are allowed to migrate under an electric current and the distance of migration is inversely cor ... | 2017 | 28608214 |
| targeting cysteine proteases in trypanosomatid disease drug discovery. | chagas disease and human african trypanosomiasis are endemic conditions in latin america and africa, respectively, for which no effective and safe therapy is available. efforts in drug discovery have focused on several enzymes from these protozoans, among which cysteine proteases have been validated as molecular targets for pharmacological intervention. these enzymes are expressed during the entire life cycle of trypanosomatid parasites and are essential to many biological processes, including i ... | 2017 | 28579388 |
| applicability of plant-based products in the treatment of trypanosoma cruzi and trypanosoma brucei infections: a systematic review of preclinical in vivo evidence. | chagas disease and sleeping sickness are neglected tropical diseases closely related to poverty, for which the development of plant-derived treatments has not been a promising prospect. thus, we systematicaly review the preclinical in vivo evidence on the applicability of plant-based products in the treatment of trypanosoma cruzi and trypanosoma brucei infections. characteristics such as disease models, treatments, toxicological safety and methodological bias were analysed. we recovered 66 full ... | 2017 | 28578742 |
| the canonical poly (a) polymerase pap1 polyadenylates non-coding rnas and is essential for snorna biogenesis in trypanosoma brucei. | the parasite trypanosoma brucei is the causative agent of african sleeping sickness and is known for its unique rna processing mechanisms that are common to all the kinetoplastidea including leishmania and trypanosoma cruzi. trypanosomes possess two canonical rna poly (a) polymerases (paps) termed pap1 and pap2. pap1 is encoded by one of the only two genes harboring cis-spliced introns in this organism, and its function is currently unknown. in trypanosomes, all mrnas, and non-coding rnas such a ... | 2017 | 28456523 |
| correction: methodological approach to the ex vivo expansion and detection of t. cruzi-specific t cells from chronic chagas disease patients. | [this corrects the article doi: 10.1371/journal.pone.0178380.]. | 2017 | 28859177 |
| synthesis of a model trisaccharide for studying the interplay between the anti α-gal antibody and the trans-sialidase reactions in trypanosoma cruzi. | trypanosoma cruzi, the etiologic agent of chagas disease, is covered by a dense glycocalix mainly composed by glycoproteins called mucins which are also the acceptors of sialic acid in a reaction catalyzed by a trans-sialidase (tcts). sialylation of trypomastigote mucins protects the parasite from lysis by the anti α-galp antibodies from serum. the tcts is essential for the infection process since t. cruzi is unable to biosynthesize sialic acid. the enzyme specifically transfers it from a termin ... | 2017 | 28858610 |
| ecological and physiological thermal niches to understand distribution of chagas disease vectors in latin america. | in order to assess how triatomines (hemiptera, reduviidae), chagas disease vectors, are distributed through latin america, we analysed the relationship between the ecological niche and the limits of the physiological thermal niche in seven species of triatomines. we combined two methodological approaches: species distribution models, and physiological tolerances. first, we modelled the ecological niche and identified the most important abiotic factor for their distribution. then, thermal toleran ... | 2017 | 28857300 |
| anti-cardiac troponin antibodies in clinical human disease: a systematic review. | anti-cardiac troponin antibodies have been studied in different types of clinical diseases and in healthy populations. a systematic review of published data on anti-troponin antibodies was carried out (search performed on pubmed, isi web of knowledge and scopus databases). from title and abstract analysis, thirty-three articles were included that met the pre-specified criteria; after full-text analysis, nine articles were excluded. most studies assessed anti-troponin i antibodies. the prevalence ... | 2017 | 28856147 |
| quantitative proteome and phosphoproteome analyses highlight the adherent population during trypanosoma cruzi metacyclogenesis. | trypanosoma cruzi metacyclogenesis is a natural process that occurs inside the triatomine vector and corresponds to the differentiation of non-infective epimastigotes into infective metacyclic trypomastigotes. the biochemical alterations necessary for the differentiation process have been widely studied with a focus on adhesion and nutritional stress. here, using a mass spectrometry approach, a large-scale phospho(proteome) study was performed with the aim of understanding the metacyclogenesis p ... | 2017 | 28852088 |
| development of conventional and real-time multiplex pcr-based assays for estimation of natural infection rates and trypanosoma cruzi load in triatomine vectors. | chagas disease is a complex anthropozoonosis with distinct domestic and sylvatic mammal species acting as potential reservoirs. the diversity of vector species and their habitats are among the factors that hinder the control of the disease. control programs periodically monitor the prevalence of t. cruzi infection in insect bugs through microscopical observation of diluted feces. however, microscopy presents limited sensitivity in samples with low parasite numbers, difficulties in examining all ... | 2017 | 28851417 |
| short-range responses of the kissing bug triatoma rubida (hemiptera: reduviidae) to carbon dioxide, moisture, and artificial light. | the hematophagous bug triatoma rubida is a species of kissing bug that has been marked as a potential vector for the transmission of chagas disease in the southern united states and northern mexico. however, information on the distribution of t. rubida in these areas is limited. vector monitoring is crucial to assess disease risk, so effective trapping systems are required. kissing bugs utilize extrinsic cues to guide host-seeking, aggregation, and dispersal behaviors. these cues have been recog ... | 2017 | 28850059 |
| new trypanosoma species, trypanosoma gennarii sp. nov., from south american marsupial in brazilian cerrado. | hundreds of trypanosome species have been described in all mammalian orders, on every continent, including with mixed infections. trypanosomes circulate in the form of sylvatic enzootic infections transmitted by blood-sucking insects that are associated with the host mammals. small wild mammals were caught in a fragment of cerrado terrain on an island in the hydroelectric reservoir of três marias, in the central region of the state of minas gerais, using pitfall and sherman traps with different ... | 2017 | 28847674 |
| evaluation of proline analogs as trypanocidal agents through the inhibition of a trypanosoma cruzi proline transporter. | trypanosoma cruzi, the etiological agent of chagas disease, uses proline as its main carbon source, essential for parasite growth and stage differentiation in epimastigotes and amastigotes. since proline is involved in many essential biological processes in t. cruzi, its transport and metabolism are interesting drug targets. | 2017 | 28844978 |
| etiological classification of stroke in patients with chagas disease using toast, causative classification system toast, and ascod phenotyping. | cardioembolism is considered a major pathophysiological mechanism in patients with ischemic stroke (is) and chagas disease (cd). however, a previous study reported that other stroke subtypes are present in more than 40% of cd patients according to the toast classification. therefore, the aim of our study was to evaluate the etiologic classification of stroke in patients with cd using the causative classification system (ccs), the ascod, and the toast classifications in a prospective cohort of pa ... | 2017 | 28844546 |
| discovery and optimization of 5-amino-1,2,3-triazole-4-carboxamide series against trypanosoma cruzi. | chagas' disease, caused by the protozoan parasite trypanosoma cruzi, is the most common cause of cardiac-related deaths in endemic regions of latin america. there is an urgent need for new safer treatments because current standard therapeutic options, benznidazole and nifurtimox, have significant side effects and are only effective in the acute phase of the infection with limited efficacy in the chronic phase. phenotypic high content screening against the intracellular parasite in infected vero ... | 2017 | 28844141 |
| automatic counting of trypanosomatid amastigotes in infected human cells. | this article presents an automatic approach to counting amastigotes in human cells infected with chagas. the approach is divided into four steps: first, morphological pretreatment removes the complex image background; sets are then segmented by unsupervised classification; the infected cells are then preserved using a thresholding process; and, finally, they undergo morphological granulometric processing and are filtered by the average. an experimental protocol was employed to compare the amasti ... | 2017 | 28841460 |
| cardiac inflammation and ventricular tachycardia in chagas disease. | 2017 | 28840107 | |
| cardiomyocyte oxidants production may signal to t. cruzi intracellular development. | chagas disease, caused by the protozoan parasite trypanosoma cruzi, presents a variable clinical course, varying from asymptomatic to serious debilitating pathologies with cardiac, digestive or cardio-digestive impairment. previous studies using two clonal t. cruzi populations, col1.7g2 (t. cruzi i) and jg (t. cruzi ii) demonstrated that there was a differential tissue distribution of these parasites during infection in balb/c mice, with predominance of jg in the heart. to date little is known a ... | 2017 | 28832582 |
| hunting hidden parasites: trypanosoma cruzi. | 2017 | 28831983 | |
| biological factors that impinge on chagas disease drug development. | chagas disease is caused by infection with the insect-transmitted protozoan trypanosoma cruzi, and is the most important parasitic infection in latin america. the current drugs, benznidazole and nifurtimox, are characterized by limited efficacy and toxic side-effects, and treatment failures are frequently observed. the urgent need for new therapeutic approaches is being met by a combined effort from the academic and commercial sectors, together with major input from not-for-profit drug developme ... | 2017 | 28831944 |
| morphometric wings similarity among sylvatic and domestic populations of triatoma infestans (hemiptera: reduviidae) from the gran chaco region of paraguay. | despite sustained efforts for eliminating triatoma infestans, reinfestation still persists in large part of the endemic area of chagas disease from the gran chaco region. sylvatic t. infestans populations seem to threat success of control programs of domestic t. infestans. in this study, we analyze whether t. infestans collected after a community-wide spraying were survivors or were immigrants from elsewhere using geometric morphometric tools. we used 101 right wings of female t. infestans captu ... | 2017 | 28829725 |
| a multi-parametric analysis of trypanosoma cruzi infection: common pathophysiologic patterns beyond extreme heterogeneity of host responses. | the extreme genetic diversity of the protozoan trypanosoma cruzi has been proposed to be associated with the clinical outcomes of the disease it provokes: chagas disease (cd). to address this question, we analysed the similarities and differences in the cd pathophysiogenesis caused by different parasite strains. using syngeneic mice infected acutely or chronically with 6 distant parasite strains, we integrated simultaneously 66 parameters: parasite tropism (7 parameters), organ and immune respon ... | 2017 | 28827716 |
| trypanocidal activity of mastoparan from polybia paulista wasp venom by interaction with tcgapdh. | chagas disease, considered a neglected disease, is a parasitic infection caused by trypanosoma cruzi, which is endemic throughout the world. previously, the antimicrobial effect of mastoparan (mp) from polybia paulista wasp venom against bacteria was described. to continue the study, we report in this short communication the antimicrobial effect of mp against trypanosoma cruzi. mp inhibits all t. cruzi developmental forms through the inhibition of tcgapdh suggested by the molecular docking. in c ... | 2017 | 28826757 |
| astrocyte apoptosis and hiv replication are modulated in host cells coinfected with trypanosoma cruzi. | the protozoan trypanosoma cruzi is the etiological agent of chagas disease. in immunosuppressed individuals, as it occurs in the coinfection with human immunodeficiency virus (hiv), the central nervous system may be affected. in this regard, reactivation of chagas disease is severe and often lethal, and it accounts for meningoencephalitis. astrocytes play a crucial role in the environment maintenance of healthy neurons; however, they can host hiv and t. cruzi. in this report, human astrocytes we ... | 2017 | 28824880 |
| mast cell coupling to the kallikrein-kinin system fuels intracardiac parasitism and worsens heart pathology in experimental chagas disease. | during the course of chagas disease, infectious forms of trypanosoma cruzi are occasionally liberated from parasitized heart cells. studies performed with tissue culture trypomastigotes (tcts, dm28c strain) demonstrated that these parasites evoke neutrophil/cxcr2-dependent microvascular leakage by activating innate sentinel cells via toll-like receptor 2 (tlr2). upon plasma extravasation, proteolytically derived kinins and c5a stimulate immunoprotective th1 responses via cross-talk between brady ... | 2017 | 28824610 |
| relation between acetylcholinesterase and na(+), k(+)-atpase activities with impaired memory of mice experimentally infected by trypanosoma cruzi. | chagas disease is caused by the protozoan parasite trypanosoma cruzi and causes severe cardiac and brain damage, leading to behavioral alterations in humans and animals. however, the mechanisms involved in memory impairment during t. cruzi infection remain unknown. it has long been recognized that the enzymatic activities of acetylcholinesterase (ache) and na(+), k(+)-atpase are linked with memory dysfunction during other trypanosomiasis. thus, the aim of this study was to evaluate the involveme ... | 2017 | 28823791 |
| a strategy for scaling up access to comprehensive care in adults with chagas disease in endemic countries: the bolivian chagas platform. | bolivia has the highest prevalence of chagas disease (cd) in the world (6.1%), with more than 607,186 people with trypanosoma cruzi infection, most of them adults. in bolivia cd has been declared a national priority. in 2009, the chagas national program (chnp) had neither a protocol nor a clear directive for diagnosis and treatment of adults. although programs had been implemented for congenital transmission and for acute cases, adults remained uncovered. moreover, health professionals were not ... | 2017 | 28820896 |
| accuracy of a rapid diagnostic test (cypress chagas quick test(®)) for the diagnosis of chronic chagas disease in a nonendemic area: a retrospective longitudinal study. | we analyzed the accuracy of chagas quick test(®), a rapid diagnostic test, for the diagnosis of chronic chagas disease through a retrospective study on a cohort of 669 patients consecutively examined at a single reference center in italy, during a 7-year period. we observed high concordance with serological reference standard but low accuracy for screening purposes (sensitivity/specificity: 82.8%/98.7%) at least in our nonendemic context. | 2017 | 28820710 |