Publications

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a participatory assessment of ecosystem services and human wellbeing in rural costa rica using photo-voice.human well-being is intricately connected to ecosystem services. a keystone contribution to the ecosystem service literature has been the millennium ecosystem assessment, ma, (ecosystems and human well-being: a framework for assessment, island press, washington, dc; 2003, 2005). much of the work on ecosystem services to date has focused on the assessment and classification of environmental functions. the need for inclusion of community perspectives in ecosystem assessments has been widely recogn ...201222402931
adsorption and kinetic behavior of recombinant multifunctional xylanase in hydrolysis of pineapple stem and bagasse and their hemicellulose for xylo-oligosaccharide production.a recombinant multifunctional xylanase (rmfxase) was utilized to hydrolyze xylan in pineapple stems and bagasse. the feasibility of using rmfxase and the two agricultural by-products for producing xylo-oligosaccharides was demonstrated. adsorption dynamics showed that lignin and cellulose delayed the adsorption equilibrium. the adsorption data fit the langmuir-type adsorption isotherm. the extent of hydrolysis was quantified by measuring the reducing and total sugars in substrate-rmfxase complex ...201222342034
effects of wax treatment on the physiology and cellular structure of harvested pineapple during cold storage.pineapple (ananas comosus (l.) merr. cv. 'paris') is sensitive to low temperature and highly susceptible to blackheart during cold storage, which causes serious fruit decay. this work investigated the effect of wax treatment (sta-fresh 2952, 60 g/l) on blackheart of pineapple under chilling stress. wax treatment significantly reduced blackheart symptoms after 14 days of storage and markedly delayed changes in firmness and flesh color during the whole period of storage. the weight loss of wax-tre ...201222681417
extensive citrus triploid hybrid production by 2x×4x sexual hybridizations and parent-effect on the length of the juvenile phase.the citrus fresh market demands the production of seedless citrus fruits, as seedy fruits are not accepted by consumers. the recovery of triploid plants has proven to be the most promising approach to achieve this goal, since triploids have very low fertility, are generally seedless and do not induce seeds in other cultivars by cross pollination. triploid plants can be recovered by 2x×4x sexual hybridization. in this work, we present an effective methodology to recover triploid plants from 2x×4x ...201222614256
study on the effect of chain-length compatibility of mixed anionic-cationic surfactants on the cloud-point extraction of selected organophosphorus pesticides.the chain-length compatibility of mixed anionic-cationic surfactants was investigated for the extraction of organophosphorus pesticides (opps). cationic surfactants with different chain lengths (n = 12 and 16) were mixed with sodium dodecyl sulfate (sds; n = 12) for the mixed anionic-cationic surfactants-based extraction. six opps were studied including azinphos-methyl, parathion-methyl, fenitrothion, diazinon, chlorpyrifos, and prothiophos. reversed-phase high-performance liquid chromatography ...201222760506
paenibacillus xylaniclasticus sp. nov., a xylanolytic-cellulolytic bacterium isolated from sludge in an anaerobic digester.a mesophilic, facultative, anaerobic, xylanolytic-cellulolytic bacterium, tw1(t), was isolated from sludge in an anaerobic digester fed with pineapple waste. cells stained gram-positive, were spore-forming, and had the morphology of straight to slightly curved rods. growth was observed in the temperature range of 30 to 50°c (optimum 37°c) and the ph range of 6.0 to 7.5 (optimum ph 7.0) under aerobic and anaerobic conditions. the strain contained meso-diaminopimelic acid in the cell-wall peptidog ...201222752902
bromelain enzyme from pineapple: in vitro activity study under different micropropagation conditions.the aim of this work was to evaluate the activity of bromelain in pineapple plants (ananas comosus var. comosus), pérola cultivar, produced in vitro in different culture conditions. this enzyme, besides its pharmacological effects, is also employed in food industries, such as breweries and meat processing. in this work, the enzymatic activity was evaluated in the tissues of leaves and stems of plants grown in culture medium without plant growth regulator. the most significant levels of bromelain ...201222736274
isolation and characterization of a flowering locus t homolog from pineapple (ananas comosus (l.) merr).flowering locus t (ft)-like genes are crucial regulators of flowering in angiosperms. a homolog of ft, designated as acft (genbank id: hq343233), was isolated from pineapple cultivar comte de paris by reverse transcriptase polymerase chain reaction (rt-pcr) and rapid amplification of cdna ends (race). the cdna sequence of acft is 915 bp in length and contains an orf of 534 bp, which encodes a protein of 177 aa. molecular weight was 19.9 kda and isoelectric point was 6.96. the deduced protein seq ...201222710136
anaerobic digestion of pineapple pulp and peel in a plug-flow reactor.the objective of this research was to study the production of biogas by using pineapple pulp and peel, the by-products from fruit processing plants, in a plug-flow reactor (17.5 l total volume). the effects of feed concentration, total solids (ts) and hydraulic retention time (hrt) on degradation of the waste were investigated. the increase of pineapple pulp and peel of 2% (wt/vol) at hrt 7 d to 4% (wt/vol) at hrt 10 d showed increases in biogas production rate, biogas yield and methane yield - ...201222705859
occupational exposure to pesticides and parkinson's disease: a systematic review and meta-analysis of cohort studies.to systematically review available cohort studies and estimate quantitatively the association between occupational exposure to pesticides and parkinson's disease (pd).201222698719
detection of postharvest changes of ascorbic acid in fresh-cut melon, kiwi, and pineapple, by using a low cost telemetric system.the present paper deals with a novel telemetric device combined with a carbon amperometric sensor system to determine postharvest changes of ascorbic acid (aa) in fresh-cut fruits, without displacing products out of the storage rooms. the investigation was performed on kiwi, pineapple and melon, subjected to minimal processing, packaging, cold storage, and simulated shelf life. results demonstrated that aa content of fresh-cut fruits of all species declines differently during storage. cold stora ...201222953893
chemical, technological and in vitro antioxidant properties of mango, guava, pineapple and passion fruit dietary fibre concentrate.the aim of this work was to determine the chemical, technological and in vitro antioxidant properties of co-products from the industrialisation of some tropical exotic fruits, such as mango, pineapple, guava and passion fruit, and to evaluate their potential use as dietary fibre sources for food enrichment. proximate compositions were determined, as well as the total, insoluble and soluble fibre contents. the water holding, oil holding and swelling capacities were also determined. for the antiox ...201222953888
expression patterns, activities and carbohydrate-metabolizing regulation of sucrose phosphate synthase, sucrose synthase and neutral invertase in pineapple fruit during development and ripening.differences in carbohydrate contents and metabolizing-enzyme activities were monitored in apical, medial, basal and core sections of pineapple (ananas comosus cv. comte de paris) during fruit development and ripening. fructose and glucose of various sections in nearly equal amounts were the predominant sugars in the fruitlets, and had obvious differences until the fruit matured. the large rise of sucrose/hexose was accompanied by dramatic changes in sucrose phosphate synthase (sps) and sucrose s ...201222949808
phenazepam and cannabinomimetics sold as herbal highs in new zealand.a smokeable product called kronic, is legally available, sold under five product names, and marketed in new zealand as containing natural extracts. two such products called pineapple express and purple haze were purchased from shops in auckland city. they were investigated for the presence of synthetic drugs specifically synthesized for recreational purposes. the synthetic cannabinomimetics identified were jwh-018, jwh-073, jwh-122, jwh-250, and 1-pentyl-3-(4-methoxybenzoyl)indol. a compound not ...201222924168
a study of lip print pattern in goan dental students - a digital approach.to find the incidence of different types of lip patterns, the dominant pattern, quadrant wise, amongst the goan population. to assess, the quadrant wise differences in lip patterns among males and females and to report new lip print pattern in goan population.201222920761
rna interference of endochitinases in the sugarcane endophyte trichoderma virens 223 reduces its fitness as a biocontrol agent of pineapple disease.the sugarcane root endophyte trichoderma virens 223 holds enormous potential as a sustainable alternative to chemical pesticides in the control of sugarcane diseases. its efficacy as a biocontrol agent is thought to be associated with its production of chitinase enzymes, including n-acetyl-ß-d-glucosaminidases, chitobiosidases and endochitinases. we used targeted gene deletion and rna-dependent gene silencing strategies to disrupt n-acetyl-ß-d-glucosaminidase and endochitinase activities of the ...201223110120
de novo assembly, characterization and functional annotation of pineapple fruit transcriptome through massively parallel sequencing.pineapple (ananas comosus var. comosus), is an important tropical non-climacteric fruit with high commercial potential. understanding the mechanism and processes underlying fruit ripening would enable scientists to enhance the improvement of quality traits such as, flavor, texture, appearance and fruit sweetness. although, the pineapple is an important fruit, there is insufficient transcriptomic or genomic information that is available in public databases. application of high throughput transcri ...201223091603
lc-ms/ms identification of a bromelain peptide biomarker from ananas comosus merr.bromelain (br) is a cysteine peptidase (genbank aeh26024.1) from pineapple, with over 40 years of clinical use. the constituents mediating its anti-inflammatory activity are not thoroughly characterized and no peptide biomarker exists. our objective is to characterize br raw material and identify peptides in the plasma of br treated mice. after sds-page in-gel digestion, br (vn#3507; middletown, ct, usa) peptides were analyzed via lc/ms/ms using 95% protein probability, 95% peptide probability, ...201223082082
environmental hazards of pesticides from pineapple crop production in the río jiménez watershed (caribbean coast, costa rica).this study aimed to characterize environmental hazards of pesticides from pineapple production in riparian communities along the jiménez river watershed. to achieve our objectives riparian ecological quality indices on riparian habitat and macroinvertebrate assemblages were combined with toxicity assays, fish biomarkers, physico-chemical water analysis and pesticide environmental hazards. during two consecutive years and two periods (july and october), three reference and four impacted sites wer ...201223040047
light and heavy dansyl reporter groups in food chemistry: amino acid assay in beverages.5-dimethylamino-1-sulfonyl naphthalene (dns, commonly referred as dansyl) is a functionality, bearing well-established properties in directing the fragmentation, by mass spectrometry (ms), of the corresponding ionized sulfonylated derivatives. this property is shared also by its labeled analogs. the use of d(0)/d(6) dns derivatives is now exploited in the application of the well-established isotope dilution mass spectrometric approach in the assay of complex mixtures. a new method for the quanti ...201222791261
effects of fibre type and kefir, wine lemon, and pineapple marinades on texture and sensory properties of wild boar and deer longissimus muscle.fibre type percentage and changes in textural parameters, sensory properties as well as mean fibre cross sectional area (csa), fibre shape, endomysium and perimysium thickness of wild boar and deer longissimus (l) muscle subjected to ageing with kefir, dry red wine, lemon and pineapple juice marinades for 4 days were studied. among the non-marinated and non-aged samples of muscles it was found that wild boar meat with its higher percentage of red fibres, higher csa, thicker connective tissue as ...201222766562
effect of mr contrast agents on quantitative accuracy of pet in combined whole-body pet/mr imaging.clinical pet/mr acquisition protocols entail the use of mr contrast agents (mrca) that could potentially affect pet quantification following mr-based attenuation correction (ac). we assessed the effect of oral and intravenous (iv) mrca on pet quantification in pet/mr imaging.201222890801
heavy metals health risk assessment for population via consumption of food crops and fruits in owerri, south eastern, nigeria.this study assessed lead, cadmium, and nickel level in food crops, fruits and soil samples from ohaji and umuagwo and owerri in south eastern nigeria and estimated the potential health risks of metals. samples were washed, oven-dried at 70-80°c for 24 h and powdered. samples were digested with perchloric acid and nitric acid. metals were analysed with unicam atomic absorption spectrophotometer.201222853175
aroma volatile compounds from two fresh pineapple varieties in china.volatile compounds from two pineapples varieties (tainong no.4 and no.6) were isolated by headspace solid phase microextraction (hs-spme) and identified and quantified by gas chromatography-mass spectrometry (gc/ms). in the tainong no. 4 and no. 6 pineapples, a total of 11 and 28 volatile compounds were identified according to their retention time on capillary columns and their mass spectra, and quantified with total concentrations of 1080.44 μg·kg(-1) and 380.66 μg·kg(-1) in the tainong no.4 an ...201222837701
microarray analysis of gene expression profiles in ripening pineapple fruits.pineapple (ananas comosus) is a tropical fruit crop of significant commercial importance. although the physiological changes that occur during pineapple fruit development have been well characterized, little is known about the molecular events that occur during the fruit ripening process. understanding the molecular basis of pineapple fruit ripening will aid the development of new varieties via molecular breeding or genetic modification. in this study we developed a 9277 element pineapple microa ...201223245313
headspace solid-phase microextraction gas chromatography-mass spectrometry and gas chromatography-olfactometry analysis of volatile compounds in pineapple breads.sensorial analysis of pineapple breads (conventionally baked, cpb; fully baked frozen, fpb and partially baked, ppb) showed no significant differences in terms of aroma and taste. on the contrary, the scores for the overall quality between the partially baked and conventionally baked breads showed significant (p < 0.05) differences. at the same time, headspace analysis using a solid-phase microextraction (spme) method identified 59 volatile compounds. the results of the aroma extracts dilution a ...201223174897
design development scopes towards occupational wellness of women workers: specific reference to local agro based food processing industries in ne india.women workers constitute one of the most vulnerable segments of the country's labour force. they often face different workplace health challenges than men do. they are engaged in a range of work that extends from heavy, monotonous, repetitive jobs, which are in many times experienced with low-paid and involves in long hours of work. women's workplace health problems are frequently compounded by getting more of the same at home--the "double jeopardy" of domestic work. specific issues to improve t ...201223151732
solution structure of a phytocystatin from ananas comosus and its molecular interaction with papain.the structure of a recombinant pineapple cystatin (accys) was determined by nmr with the rmsd of backbone and heavy atoms of twenty lowest energy structures of 0.56 and 1.11 å, respectively. it reveals an unstructured n-terminal extension and a compact inhibitory domain comprising a four-stranded antiparallel β-sheet wrapped around a central α-helix. the three structural motifs (g(45), q(89)xvxg, and w(120)) putatively responsible for the interaction with papain-like proteases are located in one ...201223139757
dna profiling of pineapple cultivars in japan discriminated by ssr markers.we developed 18 polymorphic simple sequence repeat (ssr) markers in pineapple (ananas comosus) by using genomic libraries enriched for ga and ca motifs. the markers were used to genotype 31 pineapple accessions, including seven cultivars and 11 breeding lines from okinawa prefecture, 12 foreign accessions and one from a related species. these ssr loci were highly polymorphic: the 31 accessions contained three to seven alleles per locus, with an average of 4.1. the values of expected heterozygosi ...201223341750
properties and therapeutic application of bromelain: a review.bromelain belongs to a group of protein digesting enzymes obtained commercially from the fruit or stem of pineapple. fruit bromelain and stem bromelainare prepared differently and they contain different enzymatic composition. "bromelain" refers usually to the "stem bromelain." bromelain is a mixture of different thiol endopeptidases and other components like phosphatase, glucosidase, peroxidase, cellulase, escharase, and several protease inhibitors. in vitro and in vivo studies demonstrate that ...201223304525
determination of the insecticide imidacloprid in fruit juices using micellar high-performance liquid chromatography.a simple and reliable hplc method was developed to determine imidacloprid, a chloronicotinyl insecticide that has a highly specific affinity to the nicotinic acetylcholine receptor of insects, above its permissible limit of consumption in fruit juices (orange, apple, and a mixture of pineapple and pear). samples were injected directly into a kromasil c18 column, without any pretreatment step, using the micellar mobile phase 0.10 m sodium dodecyl sulfate and 2.5% (v/v) propanol buffered at ph 7 a ...201219916392
quantification of allyl hexanoate in pineapple beverages and yogurts as a case study to characterise a source of uncertainty in dietary exposure assessment to flavouring substances.one source of uncertainty in the estimation of dietary exposure to flavouring substances is the uncertainty in the occurrence and concentration levels of these substances naturally present or added to foodstuffs. the aim of this study was to assess the variability of concentration levels of allyl hexanoate, considered as a case study, in two main food categories to which it is often added: pineapple juice-based beverages and yogurts containing pineapple. thirty-four beverages and 29 yogurts, wit ...201222043838
real-time magnetic resonance imaging of normal swallowing.to evaluate the use of a novel real-time magnetic resonance imaging (mri) technique for the assessment of normal swallowing dynamics.201222271426
amperometric fructose sensor based on ferrocyanide modified screen-printed carbon electrode.the first fructose sensor using a commercial screen-printed ferrocyanide/carbon electrodes (spfce) is reported here. the ferrocyanide is included in the carbon ink of the commercial screen-printed carbon electrode. the immobilization of enzyme d-fructose dehydrogenase (fdh) was carried out in an easy way. an aliquot of 10μl fdh was deposited on the electrode surface and left there until dried (approximately 1h) at room temperature. the sensor, so constructed, shows a good sensitivity to fructose ...201222265522
antioxidant, antimicrobial, 15-lox, and ages inhibitions by pineapple stem waste.pineapple stem has been extensively used for bromelain extraction; however, almost no attention has been given to the waste obtained during bromelain manufacturing. in this regard, antioxidant, antimicrobial, and inhibitions against 15-lipoxygenase and advanced glycation end product formations by pineapple stem waste (psw) obtained during bromelain manufacturing process were studied. the psw had moderate bioactivities in all the performed assays. it also showed a considerable inhibition against ...201222260109
high fat meal increase of il-17 is prevented by ingestion of fruit juice drink in healthy overweight subjects.an emerging role of il-17 in the inflammatory response associated with pathogenesis of neurodegeneration has been recently suggested. however, though diet represents a key factor in the modulation of inflammatory processes, evidence is not currently available on the nutritional regulation of il-17 in humans. in a double blind, randomized, placebo controlled, crossover study, we investigated the effect of high fat meal (hfm) on il-17 circulating levels in presence of a placebo (hfm-p) or with a f ...201222211683
development of ests and data mining of pineapple est-ssrs.improving the quality of the non-climacteric fruit, pineapple, is possible with information on the expression of genes that occur during the process of fruit ripening. this can be made known though the generation of partial mrna transcript sequences known as expressed sequence tags (ests). ests are useful not only for gene discovery but also function as a resource for the identification of molecular markers, such as simple sequence repeats (ssrs). this paper reports on firstly, the construction ...201222207174
[consumption of fruit juices and fruit drinks: impact on the health of children and teenagers, the dentist's point of view].the french dietary guidelines published in 2001 recommend daily consumption of 5 portions of fruit or vegetable. despite this advice, the consumption of fruit in france, especially in the north of france, is low, whereas sale of 100% fruit juices, fruit drinks, and fruit-flavored beverages is increasing. the impact of contemporary changes in beverage patterns on dental caries has received less attention than the impact on childhood obesity. nevertheless, the cariogenic potential of soft drinks i ...201222206891
bromelain-induced apoptosis in gi-101a breast cancer cells.bromelain is a proteolytic enzyme extracted from the stems and the immature fruits of pineapple that was found to be antitumorigenic in different in vitro models. bromelain has been reported to promote apoptosis, particularly in breast cancer cells, with the up-regulation of c-jun n-terminal kinase and p38 kinase. our study was designed to determine if bromelain could induce apoptosis in gi-101a breast cancer cells. gi-101a cells were treated with increasing concentrations of bromelain for 24 ho ...201222191568
effect of flammulina velutipes on spent-hen breast meat tenderization.an experiment was carried out to investigate the effects of powdered vegetable dip sauces to improve the tenderness of spent-hen breast meat. our overall purpose was to find lower-priced materials for the tenderization of spent-hen breast meat. the spent-hen breast meat was dipped into vegetable powder for 24 h at 4°c, and then the samples were analyzed. in the results for vegetable-powder treated samples, those treated with papain and pineapple had higher (p ≤ 0.05) myofibrillar fragmentation i ...201222184449
the physiology of ex vitro pineapple (ananas comosus l. merr. var md-2) as cam or c3 is regulated by the environmental conditions.many plant species grown under in vitro controlled conditions can be used as models for the study of physiological processes. adult pineapple can display cam physiology while in vitro it functions as a c3 plant. ex vitro ananas comosus has plastic morphology and physiology, both easy to modify from c3 to cam by changing the environmental conditions. the yield of survival for a rentable propagation protocol of pineapple is closely related with the c3/cam shift and the associated physiological cha ...201222134875
use of pineapple juice with gadopentetate dimeglumine as a negative oral contrast for magnetic resonance cholangiopancreatography: a multicentric study.we evaluated the efficacy of pineapple juice with gadopentetate dimeglumine as a negative oral contrast agent for magnetic resonance cholangiopancreatography (mrcp). images were obtained before and after the intake of a negative oral contrast agent. images obtained from six different areas of the biliary tree were analyzed by three different radiologists, who were blind to the exams; scores regarding image quality were given to each area. the statistical analysis showed a significant difference ...201221630051
in silico mining for simple sequence repeat loci in a pineapple expressed sequence tag database and cross-species amplification of est-ssr markers across bromeliaceae.a collection of 5,659 expressed sequence tags (ests) from pineapple [ananas comosus (l.) merr.] was screened for simple sequence repeats (est-ssrs) with motif lengths between 1 and 6 bp. lower thresholds of 15, 7 and 5 repeat units were used to define microsatellites of the mono-, di-, and tri- to hexanucleotide repeat type, respectively. based on these criteria, 696 ssrs were identified among 3,389 est unigenes, together representing 2,840 kb. this corresponds to an average density of one ssr e ...201121625993
flexural strength of fluorapatite-leucite and fluorapatite porcelains exposed to erosive agents in cyclic immersion.the aim of this study was to evaluate the flexural strength of two porcelain materials (ips d.sign and ips e.max ceram) exposed to erosive agents.201121552708
evaluation of antioxidant activity and antiproliferative effect of fruit juices enriched with pycnogenol® in colon carcinoma cells. the effect of in vitro gastrointestinal digestion.the aim of this study was to examine the effect of in vitro gastrointestinal digestion on the antioxidant and antiproliferative effect of fruit juices enriched with pycnogenol® (0.5 g/l) on a colon carcinoma cell line (caco-2). the total phenolic concentration (tpc), antioxidant activity and inhibition cell growth were studied in fresh and digested pineapple juice and red fruits juice (both enriched with pine bark extract and not). after in vitro digestion the level of detectable phenolic compou ...201121887808
growth of byssochlamys nivea in pineapple juice under the effect of water activity and ascospore age.the study of thermal resistant mould, including byssochlamys nivea, is of extreme importance since it has been associated with fruit and fruit products. the aim of this work is to analyze the influence of water activity (aw) and ascospore age (i) on the growth of byssochlamys nivea in pineapple juice. mold growth was carried out under different conditions of water activity (aw) (0.99, 0.96, 0.95, 0.93, 0.90) and ascospore age (i) (30, 51, 60, 69, 90 days). growth parameters as length of adaptati ...201124031622
modulatory effect of pineapple peel extract on lipid peroxidation, catalase activity and hepatic biomarker levels in blood plasma of alcohol-induced oxidative stressed rats.to investigate the ability of the methanolic extract of pineapple peel to modulate alcohol-induced lipid peroxidation, changes in catalase activities and hepatic biochemical marker levels in blood plasma.201123569717
polyphenol oxidase and peroxidase expression in four pineapple varieties (ananas comosus l.) after a chilling injury.pineapple internal browning (ib) is a chilling injury that produces enzymatic browning associated with flesh translucency. pineapple biodiversity allowed the investigation of how polyphenol oxidase (ppo) and peroxidase (pod) activities with their different isoforms are involved in the ib mechanism. fruits of four varieties that expressed ib symptoms differently, smooth cayenne (scay) and the hybrids md2, flhoran 41 (flh 41), and flhoran 53 (flh 53), were stressed by cold. the susceptible varieti ...201121133422
food allergy in ghanaian schoolchildren: data on sensitization and reported food allergy.epidemiological data on food allergy are scarce in african countries. we studied the prevalence of food sensitization in ghanaian schoolchildren.201121109750
in vivo genotoxicity evaluation of atrazine and atrazine-based herbicide on fish carassius auratus using the micronucleus test and the comet assay.atrazine is a selective triazine herbicide used to control broadleaf and grassy weeds mainly in corn, sorghum, sugarcane, pineapple, and other crops, and in conifer reforestation planting fields. it has been showed that atrazine is one of the most frequently detected pesticides in agricultural streams and rivers, over the past two decades. although the toxic properties of atrazine are well known, the data on the genotoxic effects of atrazine on aquatic organisms are rather scarce. thus, in the p ...201121443921
effective treatment with pineapple juice in small bowel obstruction due to phytobezoar in a gastrectomized patient. 201121429030
two phosphoenolpyruvate carboxykinases coexist in the crassulacean acid metabolism plant ananas comosus. isolation and characterization of the smaller 65 kda form.two phosphoenolpyruvate carboxykinase (pepck, ec 4.1.1.49) isoforms of 74 and 65 kda were found to coexist in vivo in pineapple leaves, a constitutive crassulacean acid metabolism plant. the 65 kda form was not the result of proteolytic cleavage of the larger form since extraction methods reported to prevent pepck proteolysis in other plant tissues failed to yield a single immunoreactive pepck polypeptide in leaf extracts. in this work, the smaller form of 65 kda was purified to homogeneity and ...201121398135
spider-fed bromeliads: seasonal and interspecific variation in plant performance.several animals that live on bromeliads can contribute to plant nutrition through nitrogen provisioning (digestive mutualism). the bromeliad-living spider psecas chapoda (salticidae) inhabits and breeds on bromelia balansae in regions of south america, but in specific regions can also appear on ananas comosus (pineapple) plantations and aechmea distichantha.201121385776
[analytical method of hydramethylnon in agricultural products by lc-ms/ms].a simple determination method of hydramethylnon in agricultural products by liquid chromatography-tandem mass spectrometry (lc-ms/ms) was developed. the sample was homogenized with phosphoric acid and extracted with acetone. an aliquot of crude extract was re-extracted with hexane and sat. nacl solution. in the case of tea leaf, solidification processing using ammonium chloride and phosphoric acid was performed before re-extraction with hexane. clean-up was performed using a silica-gel mini colu ...201121383534
effect of wine inhibitors on free pineapple stem bromelain activity in a model wine system.the influence of potential inhibitors, naturally present in wine, on the activity of stem bromelain was investigated in order to evaluate the applicability of this enzyme for protein stabilization in white wine. bromelain proteolytic activity was tested against a synthetic substrate (bz-phe-val-arg-pna) in a model wine system after adding ethanol, sulfur dioxide (so(2)), skin, seed, and gallic and ellagic tannins at the average range of their concentration in wine. all the inhibitors of stem bro ...201121381670
influence of unclogging agents on the surface state of enteral feeding tubes.despite standardized prevention procedures, recalcitrant clogging of enteral feeding tubes is observed, which requires recourse to varied unclogging agents. some of these agents have proved effective in routine use, but their impact on the surface state of the tube materials has never been studied. in this work, the authors tested the impact of different unclogging agents on the materials used for these tubes (polyurethane and silicone).201121378255
biosorption of basic green 4 from aqueous solution by ananas comosus (pineapple) leaf powder.biosorption characteristics of ananas comosus (pineapple) leaf powder was investigated for decolorization of basic green 4 (bg 4), a cationic dye from its aqueous solutions employing a batch experimental set-up. parameters that influence the sorption process such as ph, biosorbent dosage, contact time, initial dye concentration and temperature were systematically studied. the optimum conditions for removal of bg 4 were found to be ph 9.0, contact time=150 min, biosorbent dosage=5.0 g l(-1), init ...201121349693
preparation of antioxidant enzymatic hydrolysates from honeybee-collected pollen using plant enzymes.enzymatic hydrolysates of honeybee-collected pollen were prepared using food-grade proteinase and aminopeptidases entirely of plant origin. bromelain from pineapple stem was applied (8 mau/g substrate) in the first hydrolysis stage. aminopeptidase (0.05 u/g substrate) and proline iminopeptidase (0.03 u/g substrate) from cabbage leaves (brassica oleracea var. capitata), and aminopeptidase (0.2 u/g substrate) from chick-pea cotyledons (cicer arietinum l.) were involved in the additional hydrolysis ...201121318132
effect of naturally acidic agents on microhardness and surface micromorphology of restorative materials.this study investigated the titratable acidity and erosive potential of acidic agents on the microhardness and surface micromorphology of four restorative materials.201121311608
use of dispersive liquid-liquid microextraction for the determination of carbamates in juice samples by sweeping-micellar electrokinetic chromatography.dispersive liquid-liquid microextraction (dllme) has been proposed for the extraction and preconcentration of 12 carbamate pesticides in juice samples, followed by their determination by micellar electrokinetic chromatography with diode-array detection. to improve sensitivity, an on-capillary sample concentration method based on sweeping has been developed. also, separations were performed in an extended light path fused-silica capillary; the separation buffer consisted of 100 mm borate and 50 m ...201121279629
effect of acidic agents on surface roughness of dental ceramics.an increase in surface roughness of ceramics may decrease strength and affect the clinical success of ceramic restorations. however, little is known about the effect of acidic agents on ceramic restorations. the aim of this study was to evaluate the surface roughness of dental ceramics after being immersed in acidic agents.201122132009
preparative purification of bromelain (ec 3.4.22.33) from pineapple fruit by high-speed counter-current chromatography using a reverse-micelle solvent system.the crude protein of pineapple fruit was purified by high-speed counter-current chromatography (hsccc). excellent separation was achieved after careful investigation as follows: cetyltrimethylammonium bromide (ctab) was selected to prepare the reverse micelle phase at the concentration of 0.10g/ml. the mobile phase was 0.05m sodium phosphate buffer solution, including mobile phase a (ph 9.5, containing 0.2m kcl) used for equilibration and mobile phase b (ph 7.0, containing 0.4m kcl) used for elu ...201125212320
glycaemic response to some commonly eaten fruits in type 2 diabetes mellitus.it is not known which of the commonly consumed fruits in nigeria are suitable for persons with diabetes mellitus especially with regards to the attendant plasma glucose response (pgr) to consumption of such fruits.201121984455
comparison of the carbon-sequestering abilities of pineapple leaf residue chars produced by controlled combustion and by field burning.this study was undertaken to compare the chemical properties and yields of pineapple leaf residue (plr) char produced by field burning (cf) with that produced by a partial combustion of air-dried plr at 340 °c for 3 h in a furnace (cl). higher total c, lignin content, and yield from cl as well as the presence of aromatic compounds in the fourier transform infrared spectra of the char produced from cl suggest that the cl process was better in sequestering c than was the cf process. although the c ...201121958525
content of phenolic compounds in aerial parts of chamomilla suaveolens from estonia.the content of total polyphenols, flavonoids and coumarins in the aerial parts of pineapple weed (chamomilla suaveolens (pursh) rydb.), growing wild in estonia, was determined chromato-spectrophotometrically, and individual polyphenols were quantified using hplc-dad-ms/ms. the total content of polyphenols was rather similar in flowers (9.1-11.5%) and in whole aerial parts (8.4-10.9%) of pineapple weed. the upper one-third (1/3) and upper two-thirds (2/3) of the aerial parts contained more flavon ...201121922910
headspace-solid phase microextraction coupled to gas chromatography-combustion-isotope ratio mass spectrometer and to enantioselective gas chromatography for strawberry flavoured food quality control.authenticity assessment of flavoured strawberry foods was performed using headspace-solid phase microextraction (hs-spme) coupled to gas chromatography-combustion-isotope ratio mass spectrometry (gc-c-irms). an authenticity range was achieved, investigating on the carbon isotope ratio of numerous selected aroma active volatile components (methyl butanoate, ethyl butanoate, hex-(2e)-enal, methyl hexanoate, buthyl butanoate, ethyl hexanoate, hexyl acetate, linalool, hexyl butanoate, octyl isovaler ...201121872872
dissolution of undigested food in a diabetic patient's stomach by drinking pineapple juice. 201121837823
extraction of pesticides, dioxin-like pcbs and pahs in water based commodities using liquid-liquid microextraction and analysis by gas chromatography-mass spectrometry.water based samples such as flavored drinks, juices and drinking water may contain contaminants at ultra trace level belonging to different chemical classes. a novel, simple, low-cost and fast method was developed and validated for trace residue extraction of pesticides, dioxin-like pcbs and pahs from water and water based samples followed by analysis through gas chromatography (gc) coupled with time-of-flight mass spectrometry (tofms). the extraction solvent type, volume; sample volume and othe ...201121820665
quantitative analysis of essential oils in perfume using multivariate curve resolution combined with comprehensive two-dimensional gas chromatography.the use of multivariate curve resolution (mcr) to build multivariate quantitative models using data obtained from comprehensive two-dimensional gas chromatography with flame ionization detection (gc×gc-fid) is presented and evaluated. the mcr algorithm presents some important features, such as second order advantage and the recovery of the instrumental response for each pure component after optimization by an alternating least squares (als) procedure. a model to quantify the essential oil of ros ...201121704766
characteristic aroma compounds from different pineapple parts.characteristic aroma volatile compounds from different parts of cayenne pineapple were analyzed by headspace-solid phase microextraction (hs-spme) and gas chromatography-mass spectrometry (gc/ms). the main volatile compounds were esters, terpenes, ketones and aldehydes. the number and content of aroma compounds detected in pulp were higher than those found in core. in pulp, the characteristic aroma compounds were ethyl 2-methylbutanoate, ethyl hexanoate, 2,5-dimethyl-4-hydroxy-3(2h)-furanone (dm ...201121694674
identification of volatiles from pineapple (ananas comosus l.) pulp by comprehensive two-dimensional gas chromatography and gas chromatography/mass spectrometry.combining qualitative data from the chromatographic structure of 2-d gas chromatography with flame ionization detection (gc×gc-fid) and that from gas chromatography-mass spectrometry (gc/ms) should result in a more accurate assignment of the peak identities than the simple analysis by gc/ms, where coelution of analytes is unavoidable in highly complex samples (rendering spectra unsuitable for qualitative purposes) or for compounds in very low concentrations. using data from gc×gc-fid combined wi ...201121644251
reduction of voc emission from natural flours filled biodegradable bio-composites for automobile interior.various experiments, such as the thermal extract (te) method, field and emission cell (flec) method and 20 l small chamber, were performed to examine the total volatile organic compound (tvoc) emissions from bio-composites. the tvoc of neat poly(lactic acid) (pla) was ranged from 0.26 mg/m(2)h to 4.11 mg/m(2)h with increasing temperature. for both pla bio-composites with pineapple flour and destarched cassava flour, the temperature increased from 0.30 mg/m(2)h to 3.72 mg/m(2)h and from 0.19 mg/m ...201120739121
pesticide residue analysis of fruit juices by lc-ms/ms direct injection. one year pilot survey.for this work, thirteen types of fruit juices (orange, pineapple, peach, apple, multifruit, mango, strawberry, tomato, pear, mandarin, grape, banana and grapefruit) were selected to develop an analytical method for the analysis of 53 pesticides by direct injection in lc-ms/ms. the preparation of the samples was very simple: an aliquot of the juice was centrifuged and it was ten-times diluted prior to analysis, which allowed reducing considerably the time and cost of the analyses. besides, diluti ...201121238751
stability of pycnogenol® as an ingredient in fruit juices subjected to in vitro gastrointestinal digestion.the enrichment of fruit juices with concentrated polyphenolic extracts is an expedient strategy to compensate possible phenolic loss through gastrointestinal processing. pycnogenol, a standardised procyanidin-rich extract from pine bark, has been proposed as a potential candidate for polyphenol enrichment of foods. in this study the effects of in vitro digestion on the phenolic profile of fruit juices enriched with pycnogenol were investigated.201120872816
synergy between enge, xyna and mana from clostridium cellulovorans on corn stalk, grass and pineapple pulp substrates.the synergistic interaction between various hemi/cellulolytic enzymes has become more important in order to achieve effective and optimal degradation of complex lignocellulose substrates for biofuel production. this study investigated the synergistic effect of three enzymes endoglucanase (enge), mannanase (mana) and xylanase (xyna) on the degradation of corn stalk, grass, and pineapple fruit pulp and determined the optimal degree of synergy between combinations of these enzymes. it was establish ...201122558536
population dynamics of earthworms in relation to soil physico-chemical parameters in agroforestry systems of mizoram, india.earthworm population dynamics was studied in two agroforestry systems in the tropical hilly terrain of mizoram, north-east india, over a period of 24 months, from july 2002 to june 2004. two sites of agroforestry situated at sakawrtuichhun (skt) and pachhunga university college (puc) campus, aizawl, having pineapple as the main crop, were selected for detail studies on population dynamics. five of the total twelve species of earthworm reported from the state were recorded in the study sites. the ...201122319875
[development of cereal bar with pineapple skin].the cereal bars are multi-component products consisting of cereals, dried fruit and syrup binder and may be added to the consumable parts of fruits and vegetables which usually are not exploited and have high nutritional value, thereby reducing food waste. it was developed a jam with pineapple skin, which it was utilized in 13.5% in the cereal bar formulation. the cereal bar was sensorial evaluated and had its centesimal and mineral composition determined. the new product achieved average of 8.3 ...201122308949
structural interaction between gfp-labeled diazotrophic endophytic bacterium herbaspirillum seropedicae ram10 and pineapple plantlets 'vitória'.the events involved in the structural interaction between the diazotrophic endophytic bacterium herbaspirillum seropedicae, strain ram10, labeled with green fluorescent protein, and pineapple plantlets 'vitória' were evaluated by means of bright-field and fluorescence microscopy, combined with scanning electron microscopy for 28 days after inoculation. after 6 hours of inoculation, h. seropedicae was already adhered to the roots, colonizing mainly root hair surface and bases, followed by epiderm ...201124031612
a comparative study of the effects of bromelain and fresh pineapple juice on the early phase of healing in acute crush achilles tendon injury.bromelain, an enzyme extracted from the stem of the pineapple plant, has been reported to reduce pain and swelling in acute soft tissue injuries, but no study has been done to compare its effect with that of fresh pineapple juice on the healing of acute tendon injuries. this study compared the effects of commercial bromelain and fresh pineapple juice on tenocyte proliferation and the malondialdehyde (mda) level in the early stage of healing in a crush injury to the achilles tendon of sprague-daw ...201121254908
Candida krusei and Kloeckera apis inhibit the causal agent of pineapple fusariosis, Fusarium guttiforme.Studies based on microbial ecology and antagonistic interactions play an important role in the development of new alternative strategies in controlling plant pathogens and are relevant to further biotechnological applications. Antagonistic interactions between the yeasts Candida krusei and Kloeckera apis isolated from rotten pineapple fruits, and two isolates of the pathogenic filamentous fungus Fusarium guttiforme (Syn.: Fusarium subglutinans f. sp. ananas) resistant and susceptible to fungicid ...201122115444
Biotransformation of pineapple juice sugars into dietetic derivatives by using a cell free oxidoreductase from Zymomonas mobilis together with commercial invertase.An easy procedure for cell free biotransformation of pineapple juice sugars into dietetic derivatives was accomplished using a commercial invertase and an oxidoreductase from Zymomonas mobilis. First, pineapple juice sucrose was quantitatively converted into glucose and fructose by invertase, thus increasing the concentration of each monosaccharide in the original juice to almost twice. In a second step, glucose-fructose oxidoreductase (GFOR) transformed glucose into gluconolactone, and fructose ...201122112775
investigation of the factors influencing the survival of bifidobacterium longum in model acidic solutions and fruit juices.the survival of bifidobacterium longum ncimb 8809 was studied during refrigerated storage for 6weeks in model solutions, based on which a mathematical model was constructed describing cell survival as a function of ph, citric acid, protein and dietary fibre. a central composite design (ccd) was developed studying the influence of four factors at three levels, i.e., ph (3.2-4), citric acid (2-15g/l), protein (0-10g/l), and dietary fibre (0-8g/l). in total, 31 experimental runs were carried out. a ...201125212334
efficacy of xylanase purified from aspergillus niger dfr-5 alone and in combination with pectinase and cellulase to improve yield and clarity of pineapple juice.pineapple is one of the fruits having xylan rich hemicellulose content more than pectin. therefore, the efficacy of absolutely purified xylanase from a. niger dfr-5 alone and in combination with pectinase and cellulase on juice yield and clarity was studied. xylanase provided maximum yield (71.3%) and clarity (64.7%) of juice in comparison to control responses (61.8% yield and 57.8% clarity). when used together, a synergistic effect of xylanase, pectinase and cellulase on process responses was o ...201123572788
potential mechanisms of hypocholesterolaemic effect of thai spices/dietary extracts.several thai spices/dietary ingredients were previously shown to have hypocholesterolaemic effects. these studies were mostly conducted in animal models in which the mechanisms of action were not yet well-established. therefore, this study aimed to investigate the potential mechanism of hypocholesterolaemic action of 12 selected plants, namely hibiscus sabdariffa l., moringa oleifera lam., cucurbita moschata duchesne, ananas comosus (l.) merr., zingiber officinale, morus alba l., camellia sinens ...201120623425
bromelain in the early phase of healing in acute crush achilles tendon injury.bromelain, an enzyme extracted from the stem of the pineapple plant has been proposed as a treatment for reducing pain and swelling following acute muscle injuries but studies are yet to be done on its effect on tendon healing. this study therefore investigated the effects of bromelain on tenocyte proliferation and the tendon malondialdehyde (mda) level in the early stage of healing in a crush injury to the achilles tendon of sprague-dawley rats. twenty four male rats were divided randomly into ...201120623610
brief report: significant differences in perceived odor pleasantness found in children with asd.the aim of our study was to explore possible differences in estimation of odor pleasantness in children with autism spectrum disorders (asd) compared to controls. thirty-five patients with asperger's syndrome and high functioning autism (mean age 10.8 ± 3.6 years; 31 boys) were compared with 35 healthy control subjects (mean age 10.4 ± 2.4 years; 28 boys). odor pleasantness was assessed on a 5-point scale using the sniffin' sticks test (identification part of the test). patients with asd, compar ...201120697793
survival of lactobacillus plantarum in model solutions and fruit juices.the aim of the work was to study the survival of lactobacillus plantarum ncimb 8826 in model solutions and develop a mathematical model describing its dependence on ph, citric acid and ascorbic acid. a central composite design (ccd) was developed studying each of the three factors at five levels within the following ranges, i.e., ph (3.0-4.2), citric acid (6-40 g/l), and ascorbic acid (100-1000 mg/l). in total, 17 experimental runs were carried out. the initial cell concentration in the model so ...201121411170
bromelain inhibits nuclear factor kappa-b translocation, driving human epidermoid carcinoma a431 and melanoma a375 cells through g(2) /m arrest to apoptosis.bromelain, obtained from pineapple, is already in use clinically as adjunct in chemotherapy. our objective was to test its ability to act as a sole anti-cancer agent. therefore, we describe its anti-proliferative, anti-inflammatory and subsequent anti-cancer effects in vitro, against human epidermoid carcinoma-a431 and melanoma-a375 cells. bromelain exhibited reduction in proliferation of both these cell-lines and suppressed their potential for anchorage-independent growth. further, suppression ...201121432909
bromelain treatment leads to maturation of monocyte-derived dendritic cells but cannot replace pge2 in a cocktail of il-1ß, il-6, tnf-a and pge2.immunotherapy using dendritic cells (dc) has shown promising results. however, the use of an appropriate dc population is critical for the outcome of this treatment, and the search for an optimal dc subset is still ongoing. the dc used in immunotherapy today are usually matured with a cytokine cocktail consisting of tnf-a, il-1ß, il-6 and pge(2). these cells have deficits in their cytokine production, particularly il-12p70, mainly because of the presence of pge(2). bromelain is a pineapple stem ...201121449940
developmental toxicity of orally administered pineapple leaf extract in rats.the extract of pineapple leaves (epl) has anti-diabetic and anti-dyslipidemic effects and can be developed into a promising natural medicine. this study was conducted to evaluate epl's effects on developmental parameters in order to provide evidence of its safety before potential medical use. five groups were included: a negative control that was given distilled water daily, a positive control that was dosed 7 mg/kg cyclophosphamide (cp) every two days, and three groups that were respectively do ...201121457745
development and evaluation of a new method for sampling and monitoring the symphylid population in pineapple.background: symphylids (hanseniella sp.) are polyphagous soilborne parasites. today, symphylid populations on pineapple are monitored by observing root symptoms and the presence of symphylids at the bottom of basal leaves. the authors developed a reliable method with a bait and trap device to monitor symphylid populations in pineapple or fallow crops. the spatial distribution of the symphylid populations was evaluated using the variance/mean ratios and spatial analyses based on moran's and geary ...201121520484
synergistic effects of sour taste and low temperature in suppressing the bitterness of aminoleban(®) en.aminoleban(®) en, a nutritional product for patients with liver failure, contains three branched-chain amino acids (bcaas): l-leucine, l-isoleucine, and l-valine. as bcaas are extremely bitter, aminoleban(®) en has a low palatability, which is a major cause of patient noncompliance. nutrients for liver failure often need to be taken for long periods, and poor medication compliance can cause serious problems, such as encephalopathy. therefore it is important to suppress the bitter taste of aminol ...201121532188
effect of cassava starch coating on quality and shelf life of fresh-cut pineapple (ananas comosus l. merril cv "pérola").this research studied the influence of treatment with ascorbic acid, citric acid, and calcium lactate dipping and cassava starch edible coatings on quality parameters and shelf life of fresh-cut pineapple in slices during 12 d at 5 °c. after previous tests, the treatments selected for this study were samples dipped into antibrowning solution with 0.5% of ascorbic acid and 1% of citric acid, with and without 2% of calcium lactate and coated with 2% of cassava starch suspensions. changes in weight ...201121535677
enhanced iron and zinc accumulation in genetically engineered pineapple plants using soybean ferritin gene.pineapple (ananas comosus l. merr., cv. "queen") leaf bases were transformed with agrobacterium tumefaciens strain eha 105 harboring the psf and pefesf plasmids with soybean ferritin cdna. four to eight percent of the co-cultivated leaf bases produced multiple shoots 6 weeks after transfer to murashige and skoog's medium supplemented with α-naphthalene acetic acid 1.8 mg/l, indole-3-butyric acid 2.0 mg/l, kinetin 2.0 mg/l, cefotaxime 400 mg/l, and kanamycin 50 mg/l. putatively transformed shoots ...201121625916
extraction of bromelain from pineapple peels.large amount of pineapple peels (by-products) is left over after processing and they are a potential source for bromelain extraction. distilled water (di), di containing cysteine and ethylenediaminetetraacetic acid (edta) (di-ce), sodium phosphate buffer ph 7.0 (pb) and pb containing cysteine and edta (pb-ce) were used as extractants for bromelain from the pineapple peels. the highest bromelain activity was obtained when it was extracted with pb-ce (867 and 1032 units for nang lae and phu lae cu ...201121813595
genetic and phenotypic variation of fusarium proliferatum isolates from different host species.fusarium proliferatum (matsushima) nirenberg is a common pathogen infecting numerous crop plants and occurring in various climatic zones. it produces large amounts of fumonisins, a group of polyketide-derived mycotoxins. fumonisin biosynthesis is determined by the presence and activity of the fum cluster, several co-regulated genes with a common expression pattern. in the present work, we analyzed 38 f. proliferatum isolates from different host plant species, demonstrating host-specific polymorp ...201121796391
emerging risk of infestation and contamination of dried fruits by mites in the czech republic.the introduction of live insects into human food is rare in developed countries. however, we report, for the first time, an emerging risk that exists from dried fruit in central europe. recently, massive and frequent infestation of dried fruit imported from the mediterranean region by the mite, carpoglpyhus lactis l. (acarina: carpoglyphidae), has been found. in 180 samples taken from supermarkets, 13% were contaminated; the contamination levels ranged from 0 to 660 mites per g of dried fruit. t ...201121793685
effect of pineapple peel extract on total phospholipids and lipid peroxidation in brain tissues of rats.to investigate the ability of the methanolic extract of pineapple peel to attenuate alcohol-induced changes in total phospholipids and lipid peroxidation in brain tissues.201121771449
colonization of citrus seed coats by 'candidatus liberibacter asiaticus': implications for seed transmission of the bacterium.huanglongbing is an economically damaging disease of citrus associated with infection by 'ca. liberibacter asiaticus'. transmission of the organism via infection of seeds has not been demonstrated but is a concern since some citrus varieties, particularly those used as rootstocks in commercial plantings are propagated from seed. we compared the incidence of detection of 'ca. liberibacter asiaticus' dna in individual fruit peduncles, seed coats, seeds and in germinated seedlings from 'sanguenelli ...201121714779
ananas comosus: is the unripe fruit juice an abortifacient in pregnant wistar rats?abstract objective the juice of unripe pineapple (ananas comosus) was investigated for abortifacient activity in pregnant wistar rats. method animals in groups a, b, c and d received orally 0.5 ml of distilled water, 250, 500 and 1000 mg/kg body weight of the juice, respectively, once daily from day 7 until day 14 of pregnancy. results the juice contained tannins, cardenolides, dienolides, cardiac glycoside and flavonoids. the number and weights of live fetuses, number of implantation sites, cor ...201121827351
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