Publications

improved waste water treatment by bio-synthesized graphene sand composite.	the photocatalytic and antibacterial properties of graphene biosynthesized from sugar and anchored on sand particles has been focused here. the morphology and composition of the synthesized graphene sand composite (gsc) was investigated by means of x-ray powder diffraction (xrd), scanning electron microscopy (sem), energy dispersive x-ray spectroscopy (edax), fourier transform infra-red spectroscopy (ftir) and uv-visible spectroscopy. sem images show wrinkly edges. this is characteristic of grap ...	2015	26265599
improved ethanol production from biomass by a rumen metagenomic dna fragment expressed in escherichia coli ms04 during fermentation.	with the aim of improving current ethanologenic escherichia coli strains, we screened a metagenomic library from bovine ruminal fluid for cellulolytic enzymes. we isolated one fosmid, termed csd4, which was able to confer to e. coli the ability to grow on complex cellulosic material as the sole carbon source such as avicel, carboxymethyl cellulose, filter paper, pretreated sugarcane bagasse, and xylan. glucanolytic activity obtained from e. coli transformed with csd4 was maximal at 24 h of incub ...	2015	26175105
mutation in galp improved fermentation of mixed sugars to succinate using engineered escherichia coli as1600a and am1 mineral salts medium.	escherichia coli kj122 was engineered to produce succinate from glucose using the wild type galp for glucose uptake instead of the native phosphotransferase system (ptsi mutation). this strain now ferments 10% xylose poorly. mutants were selected by serial transfers in am1 mineral salts medium with 10% xylose. clones from this population all exhibited a similar improvement, co-fermentation of an equal mixture of xylose and glucose. one of these, as1600a, produced 84.26 ± 1.37 g/l succinate, equi ...	2015	26159300
overexpression and characterization of a glucose-tolerant β-glucosidase from t. aotearoense with high specific activity for cellobiose.	thermoanaerobacterium aotearoense p8g3#4 produced β-glucosidase (bgl) intracellularly when grown in liquid culture on cellobiose. the gene bgl, encoding β-glucosidase, was cloned and sequenced. analysis revealed that the bgl contained an open reading frame of 1314 bp encoding a protein of 446 amino acid residues, and the product belonged to the glycoside hydrolase family 1 with the canonical glycoside hydrolase family 1 (gh1) (β/α)8 tim barrel fold. expression of pet-bgl together with a chaperon ...	2015	25957152
the antibiotic activity and mechanisms of sugarcane (saccharum officinarum l.) bagasse extract against food-borne pathogens.	sugarcane bagasse contains natural compositions that can significantly inhibit food-borne pathogens growth. in the present study, the phenolic content in sugarcane bagasse was detected as higher than 4 mg/g dry bagasse, with 470 mg quercetin/g polyphenol. the sugarcane bagasse extract showed bacteriostatic activity against the growth of staphylococcus aureus, listeria monocytogenes, escherichia coli and salomonella typhimurium. additionally, the sugarcane bagasse extract can increase the electri ...	2015	25952848
combining treatments to improve the fermentation of sugarcane bagasse hydrolysates by ethanologenic escherichia coli ly180.	inhibitory side products from dilute acid pretreatment is a major challenge for conversion of lignocellulose into ethanol. six strategies to detoxify sugarcane hydrolysates were investigated alone, and in combinations (vacuum evaporation of volatiles, high ph treatment with ammonia, laccase, bisulfite, microaeration, and inoculum size). high ph was the most beneficial single treatment, increasing the minimum inhibitory concentration (measured by ethanol production) from 15% (control) to 70% hydr ...	2015	25864026
fvbck1, a component of cell wall integrity map kinase pathway, is required for virulence and oxidative stress response in sugarcane pokkah boeng pathogen.	fusarium verticillioides (formerly f. moniliforme) is suggested as one of the causal agents of pokkah boeng, a serious disease of sugarcane worldwide. currently, detailed molecular and physiological mechanism of pathogenesis is unknown. in this study, we focused on cell wall integrity mapk pathway as one of the potential signaling mechanisms associated with pokkah boeng pathogenesis. we identified fvbck1 gene that encodes a map kinase kinase kinase homolog and determined that it is not only requ ...	2015	26500635
tn5 insertion in the tonb gene promoter affects iron-related phenotypes and increases extracellular siderophore levels in gluconacetobacter diazotrophicus.	tonb-dependent receptors in concert with the tonb-exbb-exbd protein complex are responsible for the uptake of iron and substances such as vitamin b12 in several bacterial species. in this study, tn5 mutagenesis of the sugarcane endophytic bacterium gluconacetobacter diazotrophicus led to the isolation of a mutant with a single tn5-insertion in the promoter region of a tonb gene ortholog. this mutant, named gdiaa31, displayed a reduced growth rate and a lack of response to iron availability when ...	2015	25323530
efficient reduction of the formation of by-products and improvement of production yield of 2,3-butanediol by a combined deletion of alcohol dehydrogenase, acetate kinase-phosphotransacetylase, and lactate dehydrogenase genes in metabolically engineered klebsiella oxytoca in mineral salts medium.	klebsiella oxytoca kms005 (∆adhe∆acka-pta∆ldha) was metabolically engineered to improve 2,3-butanediol (bdo) yield. elimination of alcohol dehydrogenase e (adhe), acetate kinase a-phosphotransacetylase (acka-pta), and lactate dehydrogenase a (ldha) enzymes allowed bdo production as a primary pathway for nadh re-oxidation, and significantly reduced by-products. kms005 was screened for the efficient glucose utilization by metabolic evolution. kms005-73t improved bdo production at a concentration o ...	2015	25895450
complete genome sequence of endophytic nitrogen-fixing klebsiella variicola strain dx120e.	klebsiella variicola strain dx120e (=cgmcc 1.14935) is an endophytic nitrogen-fixing bacterium isolated from sugarcane crops grown in guangxi, china and promotes sugarcane growth. here we summarize the features of the strain dx120e and describe its complete genome sequence. the genome contains one circular chromosome and two plasmids, and contains 5,718,434 nucleotides with 57.1% gc content, 5,172 protein-coding genes, 25 rrna genes, 87 trna genes, 7 ncrna genes, 25 pseudo genes, and 2 crispr re ...	2015	26203334
saccharomyces cerevisiae transcriptional reprograming due to bacterial contamination during industrial scale bioethanol production.	the bioethanol production system used in brazil is based on the fermentation of sucrose from sugarcane feedstock by highly adapted strains of the yeast saccharomyces cerevisiae. bacterial contaminants present in the distillery environment often produce yeast-bacteria cellular co-aggregation particles that resemble yeast-yeast cell adhesion (flocculation). the formation of such particles is undesirable because it slows the fermentation kinetics and reduces the overall bioethanol yield.	2015	25633848
microencapsulation of alginate-immobilized bagasse with lactobacillus rhamnosus nrrl 442: enhancement of survivability and thermotolerance.	the aim of this research was to enhance the survivability of lactobacillus rhamnosus nrrl 442 against heat exposure via a combination of immobilization and microencapsulation processes using sugarcane bagasse (sb) and sodium alginate (naa), respectively. the microcapsules were synthesized using different alginate concentration of 1, 2 and 3% and naa:sb ratio of 1:0, 1:1 and 1:1.5. this beneficial step of probiotic immobilization before microencapsulation significantly enhanced microencapsulation ...	2015	25563958
efficient mannitol production by wild-type lactobacillus reuteri crl 1101 is attained at constant ph using a simplified culture medium.	mannitol is a natural polyol with multiple industrial applications. in this work, mannitol production by lactobacillus reuteri crl 1101 was studied at free- and controlled-ph (6.0-4.8) fermentations using a simplified culture medium containing yeast and beef extracts and sugarcane molasses. the activity of mannitol 2-dehydrogenase (mdh), the enzyme responsible for mannitol synthesis, was determined. the effect of the initial biomass concentration was further studied. mannitol production (41.5 ± ...	2015	26084891
genome-wide association and genomic prediction of resistance to maize lethal necrosis disease in tropical maize germplasm.	genome-wide association analysis in tropical and subtropical maize germplasm revealed that mlnd resistance is influenced by multiple genomic regions with small to medium effects. the maize lethal necrosis disease (mlnd) caused by synergistic interaction of maize chlorotic mottle virus and sugarcane mosaic virus, and has emerged as a serious threat to maize production in eastern africa since 2011. our objective was to gain insights into the genetic architecture underlying the resistance to mlnd b ...	2015	26152570
maize lethal necrosis (mln), an emerging threat to maize-based food security in sub-saharan africa.	in sub-saharan africa, maize is a staple food and key determinant of food security for smallholder farming communities. pest and disease outbreaks are key constraints to maize productivity. in september 2011, a serious disease outbreak, later diagnosed as maize lethal necrosis (mln), was reported on maize in kenya. the disease has since been confirmed in rwanda and the democratic republic of congo, and similar symptoms have been reported in tanzania, uganda, south sudan, and ethiopia. in 2012, y ...	2015	25822185
recombination analysis of maize dwarf mosaic virus (mdmv) in the sugarcane mosaic virus (scmv) subgroup of potyviruses.	recombination among rna viruses is a natural phenomenon that appears to have played a significant role in the species development and the evolution of many strains. it also has particular significance for the risk assessment of plants which have been genetically modified for disease resistance by incorporating viral sequences into their genomes. however, the exact recombination events taking place in viral genomes are not investigated in detail for many virus groups. in this analysis, different ...	2015	25392089
the assessment of epiphytic yeast diversity in sugarcane phyllosphere in thailand by culture-independent method.	the diversity of epiphytic yeasts from sugarcane (saccharum officinarum linn.) phyllospheres in thailand was investigated by culture-independent method based on the analysis of the d1/d2 domains of the large subunit rrna gene sequences. forty-five samples of sugarcane leaf were collected randomly from ten provinces in thailand. a total of 1342 clones were obtained from 45 clone libraries. 426 clones (31.7 %) were closely related to yeast strains in the genbank database, and they were clustered i ...	2015	26615738
thermophilic fungi as new sources for production of cellulases and xylanases with potential use in sugarcane bagasse saccharification.	to obtain new cellulases and xylanases from thermophilic fungi; evaluate their potential for sugarcane bagasse saccharification.	2015	25644433
diverse exopolysaccharide producing bacteria isolated from milled sugarcane: implications for cane spoilage and sucrose yield.	bacterial deterioration of sugarcane during harvesting and processing is correlated with significant loss of sucrose yield and the accumulation of bacterial polysaccharides. dextran, a homoglucan produced by leuconostoc mesenteroides, has been cited as the primary polysaccharide associated with sugarcane deterioration. a culture-based approach was used to isolate extracellular polysaccharide (eps) producing bacterial strains from milled sugarcane stalks. ribosomal rna sequencing analysis grouped ...	2015	26710215
antifungal properties of rhamnolipid produced by pseudomonas aeruginosa ds9 against colletotrichum falcatum.	the rhamnolipid biosurfactant (rl-ds9) extracted from the bacterial strain pseudomonas aeruginosa ds9 was evaluated for its antifungal activity against colletotrichum falcatum that causes red rot in sugarcane. the surface tension (st) reduction, biosurfactant production, and antifungal activity of biosurfactant against c. falcatum were investigated by using the medium with different carbon sources and it was found to be maximum in glucose. moreover, highest reduction of st and production of bios ...	2015	26173581
comprehensive utilization of glycerol from sugarcane bagasse pretreatment to fermentation.	in this study, the effects of glycerol pretreatment on subsequent glycerol fermentation and biomass fast pyrolysis were investigated. the liquid fraction from the pretreatment process was evaluated to be feasible for fermentation by paenibacillus polymyxa and could be an economic substrate. the pretreated biomass was further utilized to obtain levoglucosan by fast pyrolysis. the pretreated sugarcane bagasse exhibited significantly higher levoglucosan yield (47.70%) than that of un-pretreated sam ...	2015	26241838
exposure to rabies in small indian mongooses (herpestes auropunctatus) from two regions in puerto rico.	the small indian mongoose (herpestes auropunctatus) was introduced to several caribbean islands to control rat (rattus spp.) damage to sugarcane plantations. mongooses failed at suppressing rat populations and are now considered pests throughout most of their introduced range. importantly, mongooses are rabies reservoirs on several caribbean islands. in puerto rico, mongooses have been implicated in up to 70% of reported animal rabies cases. there is no rabies vaccination program for wildlife in ...	2015	26251987
isolation of pantoea ananatis from sugarcane and characterization of its potential for plant growth promotion.	each year, approximately 170 million metric tons of chemical fertilizer are consumed by global agriculture. furthermore, some chemical fertilizers contain toxic by-products and their long-term use may contaminate groundwater, lakes, and rivers. the use of plant growth-promoting bacteria may be a cost-effective strategy for partially replacing conventional chemical fertilizers, and may become an integrated plant nutrient solution for sustainable crop production. the main direct bacteria-activated ...	2015	26634494
in vitro ability of beer fermentation residue and yeast-based products to bind aflatoxin b1.	this study aimed to verify the in vitro ability of beer fermentation residue (bfr) containing saccharomyces cerevisiae cells and five commercial products that differed in the viability and integrity of s. cerevisiae cells to remove aflatoxin b1 (afb1) from a citrate-phosphate buffer solution (cpbs). bfr was collected at a microbrewery and prepared by drying and milling. the commercial yeast-based products were as follows: inactive intact yeast cells from beer alcoholic fermentation, inactive int ...	2015	26273277
statistical optimization of laccase production and delignification of sugarcane bagasse by pleurotus ostreatus in solid-state fermentation.	laccases are oxidative enzymes related to the degradation of phenolic compounds, including lignin units, with concomitant reduction of oxygen to water. delignification is a necessary pretreatment step in the process of converting plant biomass into fermentable sugars. the objective of this work was to optimize the production of laccases and to evaluate the delignification of sugarcane bagasse by pleurotus ostreatus in solid-state fermentation. among eight variables (ph, water activity, temperatu ...	2015	26180784
2g ethanol from the whole sugarcane lignocellulosic biomass.	in the sugarcane industry, large amounts of lignocellulosic residues are generated, which includes bagasse, straw, and tops. the use of the whole sugarcane lignocellulosic biomass for the production of second-generation (2g) ethanol can be a potential alternative to contribute to the economic viability of this process. here, we conducted a systematic comparative study of the use of the lignocellulosic residues from the whole sugarcane lignocellulosic biomass (bagasse, straw, and tops) from comme ...	2015	25774217
mannosylerythritol lipids: production and applications.	mannosylerythritol lipids (mels) are a glycolipid class of biosurfactants produced by a variety yeast and fungal strains that exhibit excellent interfacial and biochemical properties. mel-producing fungi were identified using an efficient screening method for the glycolipid production and taxonomical classification on the basis of ribosomal rna sequences. mel production is limited primarily to the genus pseudozyma, with significant variability among the mel structures produced by each species. o ...	2015	25748373
characterization of a protein-protein interaction network of the cbl-interacting protein kinase 8 from sugarcane.	plants are sessile organisms and have evolved to tolerate a constantly changing environment. after the onset of different stress conditions, calcineurin b-like (cbl) proteins can sense calcium signals and activate cbl-interacting protein kinase (cipk) proteins, which can phosphorylate downstream proteins to reestablish plant homeostasis. previous studies in the bioenergy crop sugarcane showed that the sccipk8 gene is induced by drought stress and is also related to sucrose content. here, we have ...	2015	25729982
production of succinic acid from sugarcane molasses supplemented with a mixture of corn steep liquor powder and peanut meal as nitrogen sources by actinobacillus succinogenes.	the potential of using corn steep liquor powder (cslp), peanut meal (pm), soybean meal (sm), cotton meal (cm) and urea as the substitute of yeast extract (ye) as the nitrogen source was investigated for producing succinic acid (sa). actinobacillus succinogenes gxas137 was used as the fermenting bacterium and sugarcane molasses was used as the main substrate. none of these materials were able to produce sa as high as ye did. the cslp could still be considered as a feasible and inexpensive alterna ...	2015	25647487
biotechnological potential of yeast isolates from cachaça: the brazilian spirit.	this study identified phenotypic traits appropriate for biotechnological applications of 118 yeasts isolated from cachaça distilleries. different properties were verified: capacity to use alternative carbon sources; ability to tolerate high concentrations of sucrose, ethanol, methanol, aluminum and zinc as well as different ph values and foam production. pichia guilliermondii and pichia anomala strains were identified as the most promising ones for application in the second-generation biofuel in ...	2015	25540045
hemicellulosic ethanol production by immobilized cells of scheffersomyces stipitis: effect of cell concentration and stirring.	bioconversion of hemicellulosic hydrolysate into ethanol plays a pivotal role in the overall success of biorefineries. for the efficient fermentative conversion of hemicellulosic hydrolysates into ethanol, the use of immobilized cells system could provide the enhanced ethanol productivities with significant time savings. here, we investigated the effect of 2 important factors (e.g., cell concentration and stirring) on ethanol production from sugarcane bagasse hydrolysate using the yeast scheffer ...	2015	25488725
mineral composition of the sugarcane juice and its influence on the ethanol fermentation.	in the present work, we evaluated the mineral composition of three sugarcane varieties from different areas in northeast brazil and their influence on the fermentation performance of saccharomyces cerevisiae. the mineral composition was homogeneous in the different areas investigated. however, large variation coefficients were observed for concentrations of copper, magnesium, zinc and phosphorus. regarding the fermentation performances, the sugarcane juices with the highest magnesium concentrati ...	2015	25248994
mathematical modeling of a continuous alcoholic fermentation process in a two-stage tower reactor cascade with flocculating yeast recycle.	experiments of continuous alcoholic fermentation of sugarcane juice with flocculating yeast recycle were conducted in a system of two 0.22-l tower bioreactors in series, operated at a range of dilution rates (d 1 = d 2 = 0.27-0.95 h(-1)), constant recycle ratio (α = f r /f = 4.0) and a sugar concentration in the feed stream (s 0) around 150 g/l. the data obtained in these experimental conditions were used to adjust the parameters of a mathematical model previously developed for the single-stage ...	2015	25234511
a one-step real-time rt-pcr assay for the detection and quantitation of sugarcane streak mosaic virus.	sugarcane mosaic disease is caused by the sugarcane streak mosaic virus (scsmv; genus poacevirus, family potyviridae) which is common in some asian countries. here, we established a protocol of a one-step real-time quantitative reverse transcription pcr (real-time qrt-pcr) using the taqman probe for the detection of scsmv in sugarcane. primers and probes were designed within the conserved region of the scsmv coat protein (cp) gene sequences. standard single-stranded rna (ssrna) generated by pcr- ...	2015	26185758
transgenic sugarcane resistant to sorghum mosaic virus based on coat protein gene silencing by rna interference.	as one of the critical diseases of sugarcane, sugarcane mosaic disease can lead to serious decline in stalk yield and sucrose content. it is mainly caused by potyvirus sugarcane mosaic virus (scmv) and/or sorghum mosaic virus (srmv), with additional differences in viral strains. rna interference (rnai) is a novel strategy for producing viral resistant plants. in this study, based on multiple sequence alignment conducted on genomic sequences of different strains and isolates of srmv, the conserve ...	2015	25685813
development of a reverse transcription loop-mediated isothermal amplification (rt-lamp) assay for the detection of sugarcane mosaic virus and sorghum mosaic virus in sugarcane.	a reverse transcription loop-mediated isothermal amplification (rt-lamp) assay was developed for detecting sugarcane mosaic virus (scmv) and sorghum mosaic virus (srmv) in sugarcane. six sets of four primers corresponding to the conserved coat protein gene were designed and tested for each virus. three primer sets designed for detecting scmv and four for detecting srmv were successful in the rt-lamp assay. the effective primer sets were not only specific for their target virus, but also able to ...	2015	25445794
characterization of sugarcane mosaic virus scmv1 and scmv2 resistance regions by regional association analysis in maize.	sugarcane mosaic virus (scmv) causes one of the most severe virus diseases in maize worldwide, resulting in reduced grain and forage yield in susceptible cultivars. in this study, two association panels consisting of 94 inbred lines each, from china and the u.s., were characterized for resistance to two isolates: scmv-seehausen and scmv-bj. the population structure of both association panels was analyzed using 3072 single nucleotide polymorphism (snp) markers. the chinese and the u.s. panel were ...	2015	26488483
sugarcane elongin c is involved in infection by sugarcane mosaic disease pathogens.	sugarcane (saccharum sp. hybrid) provides the main source of sugar for humans. sugarcane mosaic disease (smd) is a major threat to sugarcane production. currently, control of smd is mainly dependent on breeding resistant cultivars through hybridization, which is time-consuming. understanding the mechanism of viral infection may facilitate novel strategies to breed cultivars resistant to smd and to control the disease. in this study, a wide interaction was detected between the viral vpg protein a ...	2015	26362180
phosphoproteomic analysis of the resistant and susceptible genotypes of maize infected with sugarcane mosaic virus.	protein phosphorylation plays a pivotal role in the regulation of many cellular events. no information is yet available, however, on protein phosphorylation in plants in response to virus infection. in this study, we characterized phosphoproteomes of resistant and susceptible genotypes of maize (zea mays l.) in response to sugarcane mosaic virus (scmv) infection. based on isotope tags for relative and absolute quantification technology, tio2 enrichment method and lc-ms/ms analysis, we identified ...	2015	25488425
quantification of sugarcane yellow leaf virus in sugarcane following transmission through aphid vector, melanaphis sacchari.	yellow leaf caused by sugarcane yellow leaf virus (scylv) is a serious constraint to sugarcane production in india and currently the disease epidemics occur on many of the susceptible varieties under field conditions. studies were conducted on the virus transmission by sugarcane aphid melanaphis sacchari in sugarcane by inoculating virus-free meristem derived from micro- propagated plants of sugarcane cv co 86032 with viruliferous aphids. virus transmission was confirmed through rt-pcr assays an ...	2015	26645033
biology and management of sugarcane yellow leaf virus: an historical overview.	sugarcane yellow leaf virus (scylv) is one of the most widespread viruses causing disease in sugarcane worldwide. the virus has been responsible for drastic economic losses in most sugarcane-growing regions and remains a major concern for sugarcane breeders. infection with scylv results in intense yellowing of the midrib, which extends to the leaf blade, followed by tissue necrosis from the leaf tip towards the leaf base. such symptomatic leaves are usually characterized by increased respiration ...	2015	26424197
mathematical modeling of enzyme production using trichoderma harzianum p49p11 and sugarcane bagasse as carbon source.	a mathematical model to describe the kinetics of enzyme production by the filamentous fungus trichoderma harzianum p49p11 was developed using a low cost substrate as main carbon source (pretreated sugarcane bagasse). the model describes the cell growth, variation of substrate concentration and production of three kinds of enzymes (cellulases, beta-glucosidase and xylanase) in different sugarcane bagasse concentrations (5; 10; 20; 30; 40 gl(-1)). the 10 gl(-1) concentration was used to validate t ...	2015	26378961
validation of a novel sequential cultivation method for the production of enzymatic cocktails from trichoderma strains.	the development of new cost-effective bioprocesses for the production of cellulolytic enzymes is needed in order to ensure that the conversion of biomass becomes economically viable. the aim of this study was to determine whether a novel sequential solid-state and submerged fermentation method (sf) could be validated for different strains of the trichoderma genus. cultivation of the trichoderma reesei rut-c30 reference strain under sf using sugarcane bagasse as substrate was shown to be favorabl ...	2015	25399068
secretomic survey of trichoderma harzianum grown on plant biomass substrates.	the present work aims at characterizing t. harzianum secretome when the fungus is grown in synthetic medium supplemented with one of the four substrates: glucose, cellulose, xylan, and sugarcane bagasse (sb). the characterization was done by enzymatic assays and proteomic analysis using 2-de/maldi-tof and gel-free shotgun lc-ms/ms. the results showed that sb induced the highest cellulolytic and xylanolytic activities when compared with the other substrates, while remarkable differences in terms ...	2014	24593137
transcriptome profile of trichoderma harzianum ioc-3844 induced by sugarcane bagasse.	profiling the transcriptome that underlies biomass degradation by the fungus trichoderma harzianum allows the identification of gene sequences with potential application in enzymatic hydrolysis processing. in the present study, the transcriptome of t. harzianum ioc-3844 was analyzed using rna-seq technology. the sequencing generated 14.7 gbp for downstream analyses. de novo assembly resulted in 32,396 contigs, which were submitted for identification and classified according to their identities. ...	2014	24558413
molecular evolutionary history of sugarcane yellow leaf virus based on sequence analysis of rna-dependent rna polymerase and putative aphid transmission factor-coding genes.	rna-dependent rna polymerase (rdrp) encoded by orf2 and putative aphid transmission factor (patf) encoded by orf5 of sugarcane yellow leaf virus (scylv) were detected in six sugarcane cultivars affected by yellow leaf using rt-pcr and real-time rt-pcr assays. expression of both genes varied among infected plants, but overall expression of rdrp was higher than expression of patf. cultivar h87-4094 from hawaii yielded the highest transcript levels of rdrp, whereas cultivar c1051-73 from cuba exhib ...	2014	24952671
prospecting sugarcane resistance to sugarcane yellow leaf virus by genome-wide association.	using gwas approaches, we detected independent resistant markers in sugarcane towards a vectored virus disease. based on comparative genomics, several candidate genes potentially involved in virus/aphid/plant interactions were pinpointed. yellow leaf of sugarcane is an emerging viral disease whose causal agent is a polerovirus, the sugarcane yellow leaf virus (scylv) transmitted by aphids. to identify quantitative trait loci controlling resistance to yellow leaf which are of direct relevance for ...	2014	24916990
genome characterization of sugarcane yellow leaf virus from china reveals a novel recombinant genotype.	sugarcane yellow leaf virus (scylv; genus polerovirus, family luteoviridae) is a recombinant virus associated with yellow leaf disease, a serious threat to sugarcane in china and worldwide. among the nine known scylv genotypes existing worldwide, col, haw, reu, ind, chn1, chn2, bra, cub and per, the last five have been reported in china. in this study, the complete genome sequences (5,880 nt) of gz-gz18 and hn-cp502 isolates from the chinese provinces of guizhou and hainan, respectively, were cl ...	2014	24395076
characterization of small interfering rnas derived from sugarcane mosaic virus in infected maize plants by deep sequencing.	rna silencing is a conserved surveillance mechanism against viruses in plants. it is mediated by dicer-like (dcl) proteins producing small interfering rnas (sirnas), which guide specific argonaute (ago)-containing complexes to inactivate viral genomes and may promote the silencing of host mrnas. in this study, we obtained the profile of virus-derived sirnas (vsirnas) from sugarcane mosaic virus (scmv) in infected maize (zea mays l.) plants by deep sequencing. our data showed that vsirnas which d ...	2014	24819114
molecular characterization of indian sugarcane streak mosaic virus isolates reveals recombination and negative selection in the p1 gene.	sugarcane streak mosaic virus (scsmv), a member of the genus poacevirus is an important viral pathogen affecting sugarcane production in india. the p1 gene of ten indian isolates was sequenced and compared with previously reported scsmv isolates. comparative sequence analysis revealed a high level of diversity in the p1 gene (83-98% nucleotide sequence identity; 87-100% amino acid sequence identity), and the indian scsmv isolates were found to be the most variable (up to 9% diversity at the amin ...	2014	25225126
molecular variability of sugarcane streak mosaic virus in china based on an analysis of the p1 and cp protein coding regions.	sequences of the protein 1 (p1) and coat protein (cp) coding regions of 22 sugarcane streak mosaic virus (scsmv) isolates were determined. phylogenetic analysis showed that scsmv had at least three major lineages, and the lineages seemed to reflect geographical origin. the sudden expansions of the chinese and indian subpopulations were supported by calculations showing deviations from the neutral equilibrium model for the individual lineages with an overall lack of nucleotide diversity. our stud ...	2014	24178307
identification and validation of sugarcane streak mosaic virus-encoded micrornas and their targets in sugarcane.	plants have developed several defense mechanisms to cope with various pathogens (bacteria, fungi, virus, and phytoplasma). among these, rna interference (rnai)-mediated defense against viral infection was found to be a major innate immune response. as a counter attack strategy against the host defense, viruses produce suppressors of host rnai pathway. micrornas (mirnas) are an abundant class of short (~18-22 nucleotide) non-coding single-stranded rnas involved in rnai pathway leading to post-tra ...	2014	24145912
lindane degradation by candida vitjzn04, a newly isolated yeast strain from contaminated soil: kinetic study, enzyme analysis and biodegradation pathway.	a new yeast strain was isolated from sugarcane cultivation field which was able to utilize lindane as sole carbon source for growth in mineral medium. the yeast was identified and named as candida sp. vitjzn04 based on a polyphasic approach using morphological, biochemical and 18s rdna, d1/d2 and its sequence analysis. the isolated yeast strain efficiently degraded 600 mg l⁻¹ of lindane within 6 days in mineral medium under the optimal conditions (ph 7; temperature 30 °c and inoculum dosage 0.06 ...	2014	24217897
continuous ethanol production from sugarcane molasses using a newly designed combined bioreactor system by immobilized saccharomyces cerevisiae.	continuous ethanol fermentation using polyvinyl alcohol (pva), immobilized yeast, and sugarcane molasses (22 and 35°bx) with 8 g/l urea was run in a combined bioreactor system consisting of three-stage tubular bioreactors in series. the effect of the dilution rate (d) at 0.0037, 0.0075, 0.0117, 0.0145, 0.018, and 0.0282 h(-1) on continuous ethanol fermentation was investigated in this study. the results showed that d had a significant effect on fermentation efficiency, sugar-utilized rate, ethan ...	2014	24164318
variable flocculation profiles of yeast strains isolated from cachaça distilleries.	in cachaça production, the use of yeast cells as starters with predictable flocculation behavior facilitates the cell recovery at the end of each fermentation cycle. therefore, the aim of this work was to explain the behavior of cachaça yeast strains in fermentation vats containing sugarcane through the determination of biochemical and molecular parameters associated with flocculation phenotypes. by analyzing thirteen cachaça yeast strains isolated from different distilleries, our results demons ...	2014	25209588
isolation of oleaginous yeast (rhodosporidium toruloides) mutants tolerant of sugarcane bagasse hydrolysate.	rhodosporidium toruloides is a lipid-producing yeast, the growth of which is severely suppressed when hydrolysates of lignocellulosic biomass are used as carbon source. this is probably due to the toxic substances, such as organic acids, furans, and phenolic compounds produced during the preparation of the hydrolysates. in order to solve this problem, r. toruloides cultures were subjected to atmospheric room-temperature plasma mutagenesis, resulting in the isolation of mutants showing tolerance ...	2014	25036690
multi-scale structural and chemical analysis of sugarcane bagasse in the process of sequential acid-base pretreatment and ethanol production by scheffersomyces shehatae and saccharomyces cerevisiae.	heavy usage of gasoline, burgeoning fuel prices, and environmental issues have paved the way for the exploration of cellulosic ethanol. cellulosic ethanol production technologies are emerging and require continued technological advancements. one of the most challenging issues is the pretreatment of lignocellulosic biomass for the desired sugars yields after enzymatic hydrolysis. we hypothesized that consecutive dilute sulfuric acid-dilute sodium hydroxide pretreatment would overcome the native r ...	2014	24739736
evaluation of the tolerance of acetic acid and 2-furaldehyde on the growth of pichia stipitis and its respiratory deficient.	the use of lignocellulosic residues for ethanol production is limited by toxic compounds in fermenting yeasts present in diluted acid hydrolysates like acetic acid and 2-furaldehyde. the respiratory deficient phenotype gives the cell the ability to resist several toxic compounds. so the aim of this work was to evaluate the tolerance to toxic compounds present in lignocellulosic hydrolysates like acetic acid and 2-furaldehyde in pichia stipitis and its respiratory deficient strains. the respirato ...	2014	24700134
impact of nano-caco3 -ldpe packaging on quality of fresh-cut sugarcane.	in order to evaluate the effects of nano-caco3 -based low density polyethylene (nano-caco3 -ldpe) packaging on the quality of fresh-cut sugarcane, concentrations of o2 and co2 within the packages, overall visual quality (ovq), total bacterial count (tbc), yeast and mould count (ymc), reducing sugar content and total phenolic content, respiration, ethylene production, and the activities of phenylalanine ammonia-lyase (pal), polyphenol oxidase (ppo), peroxidase (pod), acid invertase (ai) and neutr ...	2014	24700113
pseudozyma brasiliensis sp. nov., a xylanolytic, ustilaginomycetous yeast species isolated from an insect pest of sugarcane roots.	a novel ustilaginomycetous yeast isolated from the intestinal tract of an insect pest of sugarcane roots in ribeirão preto, são paulo state, brazil, represents a novel species of the genus pseudozyma based on molecular analyses of the d1/d2 rdna large subunit and the internal transcribed spacer (its1+its2) regions. the name pseudozyma brasiliensis sp. nov. is proposed for this species, with ghg001(t) ( = cbs 13268(t) = ufmg-cm-y307(t)) as the type strain. p. brasiliensis sp. nov. is a sister spe ...	2014	24682702
ethanol fermentation from molasses at high temperature by thermotolerant yeast kluyveromyces sp. iipe453 and energy assessment for recovery.	high temperature ethanol fermentation from sugarcane molasses b using thermophilic crabtree-positive yeast kluyveromyces sp. iipe453 was carried out in batch bioreactor system. strain was found to have a maximum specific ethanol productivity of 0.688 g/g/h with 92 % theoretical ethanol yield. aeration and initial sugar concentration were tuning parameters to regulate metabolic pathways of the strain for either cell mass or higher ethanol production during growth with an optimum sugar to cell rat ...	2014	24682264
selecting low-cost carbon sources for carotenoid and lipid production by the pink yeast rhodosporidium toruloides ncyc 921 using flow cytometry.	the present work studied low-cost carbon sources for carotenoid and lipid production using the yeast rhodosporidum toruloides ncyc 921. carob pulp syrup and sugarcane molasses at different concentrations were used as low-cost carbon sources in r. toruloides batch cultivations. carob pulp syrup containing a total sugar concentration of 75 g l(-1) induced the highest total fatty acid productivity (1.90 g l(-1)h(-1)) and the highest carotenoid productivity (9.79 μg l(-1)h(-1)). flow cytometric anal ...	2014	24650616
effect of feeding strategies on lipid production by lipomyces starkeyi.	the aim of this study was to produce microbial oil from lipomyces starkeyi dsm 70296 grown in hemicellulose hydrolysate (h-h). glucose and xylose were used for batch, fed-batch, repeated fed-batch, and continuous cultures, and h-h was tested at continuous culture. the highest cell and lipid concentrations of 85.4 and 41.8g/l, respectively, were obtained using repeated fed-batch strategy. continuous culture with dilution rate of 0.03h(-1) presented the highest overall cell (0.443g/g) and lipid yi ...	2014	24556374
statistical design and optimization of single cell oil production from sugarcane bagasse hydrolysate by an oleaginous yeast rhodotorula sp. iip-33 using response surface methodology.	single cell oil production from sugarcane bagasse hydrolysate by oleaginous yeast rhodotorula sp. iip-33 was analyzed using a two stage statistical design approach based on response surface methodology. variables like pentose sugar, (nh4)2so4, kh2po4, yeast extract, ph and temperature were found to influence lipid production significantly. under optimized condition in a shake flask, yield of lipid was 2.1199 g with fat coefficient of 7.09 which also resembled ~99% similarity to model predicted l ...	2014	26034686
new cultive medium for bioconversion of c5 fraction from sugarcane bagasse using rice bran extract.	the use of hemicellulosic hydrolysates in bioprocesses requires supplementation as to ensure the best fermentative performance of microorganisms. however, in light of conflicting data in the literature, it is necessary to establish an inexpensive and applicable medium for the development of bioprocesses. this paper evaluates the fermentative performance of scheffersomyces (pichia) stipitis and candida guilliermondii growth in sugarcane bagasse hemicellulosic hydrolysate supplemented with differe ...	2014	25763056
the fermentation of sugarcane molasses by dekkera bruxellensis and the mobilization of reserve carbohydrates.	the yeast dekkera bruxellensis is considered to be very well adapted to industrial environments, in brazil, usa, canada and european countries, when different substrates are used in alcoholic fermentations. our previous study described its fermentative profile with a sugarcane juice substrate. in this study, we have extended its physiological evaluation to fermentation situations by using sugarcane molasses as a substrate to replicate industrial working conditions. the results have confirmed the ...	2014	24370978
comparative genomic analysis of saccharomyces cerevisiae yeasts isolated from fermentations of traditional beverages unveils different adaptive strategies.	saccharomyces cerevisiae strains are the main responsible of most traditional alcohol fermentation processes performed around the world. the characteristics of the diverse traditional fermentations are very different according to their sugar composition, temperature, ph or nitrogen sources. during the adaptation of yeasts to these new environments provided by human activity, their different compositions likely imposed selective pressures that shaped the s. cerevisiae genome. in the present work ...	2014	24334254
brewer's yeast and sugarcane yeast as protein sources for dogs.	brewer's yeast (by), autolysed sugarcane yeast (ascy) and integral sugar cane yeast (iscy) were studied in two experiments as ingredients for dog diets. in the first experiment, 28 dogs were randomly assigned to four diets; one reference diet and three test diets containing 15% of by, ascy or iscy and 85% of the reference diet (as-fed basis). the digestibilities of the yeasts were calculated by the substitution method. in the second experiment, 35 dogs were randomized to five diets with similar ...	2014	24304448
ethanol production from agricultural wastes using saccharomyces cerevisiae.	the main objective of this study was production of ethanol from three lignocellulosic biomasses like sugarcane bagasse, rice straw and wheat straw by sacchromyces cervisae. all the three substrates were ground to powder form (2 mm) and pretreated with 3%h2o2 + 2% naoh followed by steaming at 130 °c for 60 min. these substrates were hydrolyzed by commercial cellulase enzyme. the whole fermentation process was carried out in 500 ml erlenmeyer flask under anaerobic conditions in submerged fermentat ...	2014	25242928
optimization of lipids production by cryptococcus laurentii 11 using cheese whey with molasses.	this study aimed the optimization of culture condition and composition for production of cryptococcus laurentii 11 biomass and lipids in cheese whey medium supplemented with sugarcane molasses. the optimization of ph, fermentation time, and molasses concentration according to a full factorial statistical experimental design was followed by a plackett-burman experimental design, which was used to determine whether the supplementation of the culture medium by yeast extract and inorganic salts coul ...	2014	25242919
differential responses of the antioxidant system of ametryn and clomazone tolerant bacteria.	the herbicides ametryn and clomazone are widely used in sugarcane cultivation, and following microbial degradation are considered as soil and water contaminants. the exposure of microorganisms to pesticides can result in oxidative damage due to an increase in the production of reactive oxygen species (ros). this study investigated the response of the antioxidant systems of two bacterial strains tolerant to the herbicides ametryn and clomazone. bacteria were isolated from soil with a long history ...	2014	25380132
a glucuronoxylan-specific xylanase from a new paenibacillus favisporus strain isolated from tropical soil of brazil.	a new xylanolytic strain, paenibacillus favisporus cc02-n2, was isolated from sugarcane plantation fields in brazil. the strain had a xylan-degrading system with multiple enzymes, one of which, xylanase xyn30a, was identified and characterized. the enzyme is a single-domain xylanase belonging to family 30 of the glycosyl hydrolases (gh30). xyn30a shows high activity on glucuronoxylans, with a vmax of 267.2 u mg⁻¹, a km of 4.0 mg/ml, and a kcat of 13,333 min⁻¹ on beechwood xylan, but it does not ...	2014	26419457
filamentous fungi and media for cellulase production in solid state cultures.	cellulase production was evaluated in two reference strains (t. reesei rut-c30 and t. reesei qm9414), two strains isolated from a sugarcane cultivation area (trichoderma sp. ipt778 and t. harzianum rifai ipt821) and one strain isolated in a program for biodiversity preservation in são paulo state (myceliophthora thermophila m77). solid state cultures were performed using sugarcane bagasse (c), wheat bran (w) and/or soybean bran (s). the highest fpa was 10.6 u/gdm for m77 in sc (10:90) at 80% moi ...	2014	24948946
organic loading rate impact on biohydrogen production and microbial communities at anaerobic fluidized thermophilic bed reactors treating sugarcane stillage.	this study aimed to evaluate the effect of high organic loading rates (olr) (60.0-480.00 kg cod m(-3)d(-1)) on biohydrogen production at 55°c, from sugarcane stillage for 15,000 and 20,000 mg codl(-1), in two anaerobic fluidized bed reactors (afbr1 and afbr2). it was obtained, for h2 yield and content, a decreasing trend by increasing the olr. the maximum h2 yield was observed in afbr1 (2.23 mmol g cod added(-1)). the volumetric h2 production was proportionally related to the applied hydraulic r ...	2014	24632626
genetic analysis of resistance to six virus diseases in a multiple virus-resistant maize inbred line.	novel and previously known resistance loci for six phylogenetically diverse viruses were tightly clustered on chromosomes 2, 3, 6 and 10 in the multiply virus-resistant maize inbred line, oh1vi. virus diseases in maize can cause severe yield reductions that threaten crop production and food supplies in some regions of the world. genetic resistance to different viruses has been characterized in maize populations in diverse environments using different screening techniques, and resistance loci hav ...	2014	24500307
a high degree of african streak virus diversity within nigerian maize fields includes a new mastrevirus from axonopus compressus.	the a-strain of maize streak virus (msv-a; genus mastrevirus, family geminiviridae), the causal agent of maize streak disease, places a major constraint on maize production throughout sub-saharan africa. in west-african countries such as nigeria, where maize is not cultivated year-round, this msv strain is forced to overwinter in non-maize hosts. in order to both identify uncultivated grasses that might harbour msv-a during the winter season and further characterise the diversity of related maiz ...	2014	24796552
biohydrogen production from sugarcane bagasse by integrating dark- and photo-fermentation.	hydrogen production from sugarcane bagasse (scb) by integrating dark-fermentation by enterobacter aerogenes mtcc 2822 and photo-fermentation by rhodopseudomonas bhu 01 was investigated. the scb was hydrolysed by sulphuric acid and the hydrolysate detoxified by passing through adsorbent resin column (amberlite xad-4) to remove the inhibitory furfural, and subjected to dark-fermentation. the cellulosic residue from acid hydrolysis was hydrolysed by the new isolate cellulomonas fimi to release suga ...	2014	24291314
homo- and heterofermentative lactobacilli differently affect sugarcane-based fuel ethanol fermentation.	bacterial contamination during industrial yeast fermentation has serious economic consequences for fuel ethanol producers. in addition to deviating carbon away from ethanol formation, bacterial cells and their metabolites often have a detrimental effect on yeast fermentative performance. the bacterial contaminants are commonly lactic acid bacteria (lab), comprising both homo- and heterofermentative strains. we have studied the effects of these two different types of bacteria upon yeast fermentat ...	2014	24198118
a rapid and visual loop-mediated isothermal amplification assay to detect leifsonia xyli subsp. xyli targeting a transposase gene.	leifsonia xyli subsp. xyli (lxx), causal organism of ratoon stunt (rsd), does not produce any reliable internal or external symptoms on sugarcane. its detection on a large scale is solely based on microscopic and serological methods. these methods require well-equipped laboratories, are time consuming and are not feasible for near-field detection of lxx. in this study, we developed a loop-mediated isothermal amplification (lamp) assay for rapid and sensitive detection of lxx without the use of s ...	2014	25201631
[growth-promoting effect of inoculating klebsiella variicola dx120e on different sugarcane cultivars].	in order to investigate the growth promoting effect of inoculating klebsiella variicola dx120e, a bacterial strain with high activity of associative nitrogen fixation, on sugarcane, the strain was inoculated through roots into the pathogen free micropropagated seedlings of two sugarcane cultivars b8 and gt21. the bacterial numbers colonized in sugarcane plants, the activities of the key enzymes for nitrogen metabolism, the nitrate concentration and nutrient uptake were analyzed. the results indi ...	2014	25345062
sustainable conversion of coffee and other crop wastes to biofuels and bioproducts using coupled biochemical and thermochemical processes in a multi-stage biorefinery concept.	the environmental impact of agricultural waste from the processing of food and feed crops is an increasing concern worldwide. concerted efforts are underway to develop sustainable practices for the disposal of residues from the processing of such crops as coffee, sugarcane, or corn. coffee is crucial to the economies of many countries because its cultivation, processing, trading, and marketing provide employment for millions of people. in coffee-producing countries, improved technology for treat ...	2014	25204861
draft genome sequence of kluyveromyces marxianus strain dmb1, isolated from sugarcane bagasse hydrolysate.	we determined the genome sequence of a thermotolerant yeast, kluyveromyces marxianus strain dmb1, isolated from sugarcane bagasse hydrolysate, and the sequence provides further insights into the genomic differences between this strain and other reported k. marxianus strains. the genome described here is composed of 11,165,408 bases and has 4,943 protein-coding genes.	2014	25059876
modeling the microbial growth and temperature profile in a fixed-bed bioreactor.	aiming to scale up and apply control and optimization strategies, currently is required the development of accurate plant models to forecast the process nonlinear dynamics. in this work, a mathematical model to predict the growth of the kluyveromyces marxianus and temperature profile in a fixed-bed bioreactor for solid-state fermentation using sugarcane bagasse as substrate was built up. a parameter estimation technique was performed to fit the mathematical model to the experimental data. the es ...	2014	24658796
optimization, kinetics, and modeling of inulinase production by k. marxianus var. marxianus.	pressmud, a by-product from the sugarcane industry, was used as a carbon source for the production of inulinase in solid-state fermentation (ssf). statistical experimental designs were employed to screen the nutrients and optimize the media composition for the production of inulinase by kluyveromyces marxianus var. marxianus. eighteen various nutrients were selected for preliminary screening of production medium component by plackett-burman design (pbd) technique. five nutrients were found to be ...	2014	24274017
optimization of endoglucanase and xylanase activities from fusarium verticillioides for simultaneous saccharification and fermentation of sugarcane bagasse.	enzymatic hydrolysis is an important but expensive step in the production of ethanol from biomass. thus, the production of efficient enzymatic cocktails is of great interest for this biotechnological application. the production of endoglucanase and xylanase activites from f. verticillioides were optimized in a factorial design (2(5)) followed by a ccdr design. endoglucanase and xylanase activities increased from 2.8 to 8.0 u/ml and from 13.4 to 114 u/ml, respectively. the optimal ph and temperat ...	2014	24170331
rhamnolipid biosurfactant against fusarium sacchari--the causal organism of pokkah boeng disease of sugarcane.	pokkah boeng disease on sugarcane caused by the fungus fusarium sacchari results considerable damage to the crop leading to top rot, the most serious and advanced stage of pokkah boeng, where the growing point is killed and the entire top of the plant dies. in the present study, the effect of rhamnolipid biosurfactant as an antifungal agent against f. sacchari to control pokkah boeng disease was investigated. on the basis of surface tension reduction, 12 bacterial isolates were selected as poten ...	2014	23687052
effect of fiber digestibility and conservation method on feed intake and the ruminal ecosystem of growing steers.	fiber digestibility is an important factor regulating dmi in ruminants. additionally, the ensiling process can also affect digestibility and chemical composition of the forage. the objective of this study was to investigate effects of sugarcane ndf digestibility (ndfd) and conservation method on intake, rumen kinetics, and the ruminal ecosystem of steers. eight ruminally cannulated nellore steers (275±22 kg bw) were used in a replicated 4×4 latin square design with a 2×2 factorial arrangement of ...	2014	25414108
escherichia coli w shows fast, highly oxidative sucrose metabolism and low acetate formation.	sugarcane is the most efficient large-scale crop capable of supplying sufficient carbon substrate, in the form of sucrose, needed during fermentative feedstock production. however, sucrose metabolism in escherichia coli is not well understood because the two most common strains, e. coli k-12 and b, do not grow on sucrose. here, using a sucrose utilizing strain, e. coli w, we undertake an in-depth comparison of sucrose and glucose metabolism including growth kinetics, metabolite profiling, microa ...	2014	25125039
screening of lactic acid bacteria for their potential as microbial cell factories for bioconversion of lignocellulosic feedstocks.	the use of fossil carbon sources for fuels and petrochemicals has serious impacts on our environment and is unable to meet the demand in the future. a promising and sustainable alternative is to substitute fossil carbon sources with microbial cell factories converting lignocellulosic biomass into desirable value added products. however, such bioprocesses require availability of suitable and efficient microbial biocatalysts, capable of utilizing c5 sugars and tolerant to inhibitory compounds gene ...	2014	24997803
one-pot green synthesis of carbon dots by using saccharum officinarum juice for fluorescent imaging of bacteria (escherichia coli) and yeast (saccharomyces cerevisiae) cells.	we are reporting highly economical plant-based hydrothermal method for one-pot green synthesis of water-dispersible fluorescent carbon dots (cds) by using saccharum officinarum juice as precursor. the synthesized cds were characterized by uv-visible, fluorescence, fourier transform infrared (ft-ir), dynamic light scattering (dls), high-resolution transmission electron microscopic (hr-tem), and laser scanning confocal microscopic techniques. the cds are well dispersed in water with an average siz ...	2014	24656348
isolation of a novel peroxisomal catalase gene from sugarcane, which is responsive to biotic and abiotic stresses.	catalase is an iron porphyrin enzyme, which serves as an efficient scavenger of reactive oxygen species (ros) to avoid oxidative damage. in sugarcane, the enzymatic activity of catalase in a variety (yacheng05-179) resistant to the smut pathogen sporisorium scitamineum was always higher than that of the susceptible variety (liucheng03-182), suggesting that catalase activity may have a positive correlation with smut resistance in sugarcane. to understand the function of catalase at the molecular ...	2014	24392135
isolation and identification of hexazinone-degrading bacterium from sugarcane-cultivated soil in kenya.	the s-triazine herbicide hexazinone [3-cyclohexyl-6-dimethylamino-1-methyl-1,3,5-triazine-2,4(1h,3h)-dione], is widely used in agriculture for weed control. laboratory biodegradation experiments for hexazinone in liquid cultures were carried out using sugarcane-cultivated soils in kenya. liquid culture experiments with hexazinone as the only carbon source led to the isolation of a bacterial strain capable of its degradation. through morphological, biochemical and molecular characterization by 16 ...	2014	24458247
biological neutralization and biosorption of dyes of alkaline textile industry wastewater.	the present work was aimed to secure biological neutralization and biosorption of dyes of an alkaline textile industrial effluent (atie) using an alkaliphilic bacterium, enterococcus faecalis strain r-16 isolated from gujarat coast. the isolate was capable and competent to bring down the ph of atie from 12.1 to 7.0 within 2 h in the presence of carbon and nitrogen sources. carboxylic group concentration (cgc), nmr and ft-ir analysis revealed production of carboxylic acid as a result of neutraliz ...	2014	24910184
extended monitoring of resistance to bacillus thuringiensis cry1ab maize in diatraea saccharalis (lepidoptera: crambidae).	the sugarcane borer, diatraea saccharalis (f.), is a major target of transgenic maize expressing bacillus thuringiensis (bt) proteins in south america and the mid-southern region of the united states. during 2007-2009, a total of 986 feral individuals of d. saccharalis were collected from maize fields in six locations of louisiana and mississippi and examined for resistance to cry1ab maize using f 1/f 2 screens. major resistance alleles to cry1ab maize in the populations sampled from non-bt maiz ...	2014	22688686
draft genome sequence of xanthomonas axonopodis pathovar vasculorum ncppb 900.	xanthomonas axonopodis pathovar vasculorum strain ncppb 900 was isolated from sugarcane on reunion island in 1960. consistent with its belonging to fatty-acid type d, multi-locus sequence analysis confirmed that ncppb 900 falls within the species x. axonopodis. this genome harbours sequences similar to plasmids pxcv183 from x. campestris pv. vesicatoria 85-10 and pphb194 from burkholderia pseudomallei. its repertoire of predicted effectors includes homologues of xopaa, xopad, xopae, xopb, xopd, ...	2014	25263632
maize elongin c interacts with the viral genome-linked protein, vpg, of sugarcane mosaic virus and facilitates virus infection.	the viral genome-linked protein, vpg, of potyviruses is involved in viral genome replication and translation. to determine host proteins that interact with sugarcane mosaic virus (scmv) vpg, a yeast two-hybrid screen was used and a maize (zea mays) elongin c (zmelc) protein was identified. zmelc transcript was observed in all maize organs, but most highly in leaves and pistil extracts, and zmelc was present in the cytoplasm and nucleus of maize cells in the presence or absence of scmv. zmelc exp ...	2014	24954157
diversity of culturable yeasts in phylloplane of sugarcane in thailand and their capability to produce indole-3-acetic acid.	yeasts were isolated by the enrichment technique from the phylloplane of 94 samples of sugarcane leaf collected from seven provinces in thailand. all sugarcane leaf samples contained yeasts and 158 yeast strains were obtained. on the basis of the d1/d2 domain of the large subunit rrna gene sequence analysis, 144 strains were identified to 24 known species in 14 genera belonging to the ascomycota viz. candida akabanensis, candida dendronema, candida mesorugosa, candida michaelii, candida nivarien ...	2014	24442819
control of diatraea saccharalis by the endophytic pantoea agglomerans 33.1 expressing cry1ac7.	despite the fact that bacillus thuringiensis (bt) is found in more than 90 % of the products used against insects, it has some difficulty reaching the internal regions where the larvae feed. to solve this problem, many genetically modified microorganisms that colonize the same pests have been developed. thus, the endophytic bacterium pantoea agglomerans (33.1), which has been recently described as a promising sugarcane growth promoter, was genetically modified with the pjtt vector (which carries ...	2014	24531524
fitness costs and stability of cry1ab resistance in sugarcane borer, diatraea saccharalis (f.).	the sugarcane borer, diatraea saccharalis (f.), is a major target species of transgenic corn expressing bacillus thuringiensis (bt) proteins in south america and the u.s. mid-south region. in this study, the fitness of seven insect genotypes of d. saccharalis were assayed on non-toxic diet, which included a cry1ab-susceptible strain (ss-2009), two cry1ab-resistant strains (rr-43a(bc), rr-l5b(bc)), and four f1 hybrids (f1-r43a(m)s(f), f1-r43a(f)s(m), f1-r5b(m)s(f), and f1-r5b(f)s(m)). the f1 hybr ...	2014	24503242
modeling evolution of resistance of sugarcane borer (lepidoptera: crambidae) to transgenic bt corn.	diatraea saccharalis (f.) (lepidoptera: crambidae) is a target pest of transgenic corn expressing bacillus thuringiensis (bt) protein, and the first evidence of resistance by d. saccharalis to cry1ab corn was detected in a field population in northeast louisiana in 2004. we used a model of population dynamics and genetics of d. saccharalis to 1) study the effect of interfield dispersal, the first date that larvae enter diapause for overwintering, toxin mortality, the proportion of non-bt corn in ...	2014	24914780