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3-methylthiopropionic acid ethyl ester, isolated from katsura-uri (japanese pickling melon, cucumis melo var. conomon), enhanced differentiation in human colon cancer cells.the fully ripened fruit of katsura-uri japanese pickling melon ( cucumis melo var. conomon) has rarely been used for food because the midripened fruit is utilized for making pickles, but the fully ripened fruit is no longer valuable for pickles due to the fruit body being too soft. we have considered the utilization of the fully ripened katsura-uri fruit that may be used for nonpickling products, particularly if the fully ripened fruit demonstrated health benefits such as anticarcinogenic proper ...200818426216
antioxidant, sugar, mineral, and phytonutrient concentrations across edible fruit tissues of orange-fleshed honeydew melon (cucumis melo l.).orange-fleshed, non-netted honeydew ( cucumis melo l.) is a relatively new melon in the marketplace and has shown a lot of potential as an alternative to netted muskmelons (cantaloupes), which are often prone to surface contamination by enteric bacteria. orange-fleshed honeydew is a cross between orange-fleshed cantaloupe and non-netted, green-fleshed honeydew. this glasshouse study investigated the nutritional profile (phytonutrient and sugar contents) in different tissues of mature orange-fles ...200818454549
reduction by gaseous ozone of salmonella and microbial flora associated with fresh-cut cantaloupe.this research investigates the efficacy of gaseous ozone, applied under partial vacuum in a controlled reaction chamber, for the elimination of salmonella inoculated on melon rind. the performance of high dose, short duration treatment with gaseous ozone, in this pilot system, on the microbial and sensory quality of fresh-cut cantaloupes was also evaluated. gaseous ozone (10,000 ppm for 30 min under vacuum) reduced viable, recoverable salmonella from inoculated physiologically mature non-ripe an ...200818456110
survival of sa11 rotavirus in fresh fruit juices of pineapple, papaya, and honeydew melon.survival of rotavirus in fresh fruit juices of papaya (caraca papaya l.), honeydew melon (cucumis melo l.), and pineapple (ananas comosus [l.] merr.) was studied. clarified juices were prepared from pulps of ripe fruits and sterilized by ultrafiltration. one milliliter of juice from each fruit was inoculated with 20 microl of 1 x 10(6) pfu of sa11 rotavirus and sampled immediately (0-h exposure) and 1 and 3 h later at 28 degrees c. mean viral titers in juices of papaya (ph 5.1) and honeydew melo ...200818522042
effect of gaseous ozone and hot water on microbial and sensory quality of cantaloupe and potential transference of escherichia coli o157:h7 during cutting.the effect of gaseous ozone and hot water, alone or in combination, on the sensory and microbial quality of cantaloupe melon was investigated. escherichia coli o157:h7 transmission from the rind to edible melon flesh during cutting practices was also investigated. four different treatments consisting of hot water (75 degrees c, 1min), gaseous ozone (10,000ppm, 30min), gaseous ozone supplied by carbon monoxide gas and the combination of hot water and gaseous ozone were evaluated. sensory quality ...200817993390
possible amelioration of atherogenic diet induced dyslipidemia, hypothyroidism and hyperglycemia by the peel extracts of mangifera indica, cucumis melo and citrullus vulgaris fruits in rats.hitherto unknown efficacy of the peel extracts of mangifera indica (mi), cucumis melo (cm) and citrullus vulgaris (cv) fruits in ameliorating the diet-induced alterations in dyslipidemia, thyroid dysfunction and diabetes mellitus have been investigated in rats. in one study, out of 4 different doses (50-300 mg/kg), 200 mg/kg of mi and 100 mg/kg for other two peel extracts could inhibit lipidperoxidation (lpo) maximally in liver. in the second experiment rats were maintained on pre-standardized a ...200819276533
a new strain of melon necrotic spot virus that is unable to systemically infect cucumis melo.we report a new strain of melon necrotic spot virus (mnsv) that is unable to systemically infect cucumis melo. a spherical virus (w-isolate), about 30 nm in diameter like a carmovirus, was isolated from watermelons with necrotic symptoms. the w-isolate had little serological similarity to mnsv, and it did not cause any symptoms in six melon cultivars susceptible to mnsv; however, the host range of the w-isolate was limited exclusively to cucurbitaceous plants, and transmission by o. bornovanus w ...200818943404
recovery from cucurbit leaf crumple virus (family geminiviridae, genus begomovirus) infection is an adaptive antiviral response associated with changes in viral small rnas.a strong recovery response occurs in cantaloupe (cucumis melo) and watermelon (citrullus lanatus) infected with the bipartite begomovirus cucurbit leaf crumple virus (culcrv). this response is characterized by initially severe symptoms, which gradually become attenuated (almost symptomless). an inverse relationship was detected between viral dna levels and recovery, indicating that recovered tissues had reduced viral titers. recovered tissues also were resistant to reinfection with culcrv; i.e., ...200818943741
mapping of genetic loci that regulate quantity of beta-carotene in fruit of us western shipping melon (cucumis melo l.).melon (cucumis melo l.) is highly nutritious vegetable species and an important source of beta-carotene (vitamin a), which is an important nutrient in the human diet. a previously developed set of 81 recombinant inbred lines (ril) derived from group cantalupensis us western shipper market type germplasm was examined in two locations [wisconsin (wi) and california (ca), usa] over 2 years to identify quantitative trait loci (qtl) associated with quantity of beta-carotene (qbetac) in mature fruit. ...200818773190
edible alginate-based coating as carrier of antimicrobials to improve shelf-life and safety of fresh-cut melon.the effect of malic acid and essential oils (eos) of cinnamon, palmarosa and lemongrass and their main active compounds as natural antimicrobial substances incorporated into an alginate-based edible coating on the shelf-life and safety of fresh-cut "piel de sapo" melon (cucumis melo l.) was investigated. melon pieces (50 g) were coated with alginate-based edible coating containing malic acid (ec) and eos or their active compounds before to be packed in air filled polypropylene trays and stored a ...200818164505
complete nucleotide sequence of a new isolate of tomato leaf curl new delhi virus infecting cucumber, bottle gourd and muskmelon in thailand. 200818193155
long-distance trafficking of macromolecules in the phloem.the fact that macromolecules such as proteins and mrnas overcome the symplastic barriers between various tissue domains was first evidenced by the movement of plant viruses. we have recently demonstrated that viral infection disengages the symplastic restriction present between the sieve element-companion cell complex and neighboring cells in tobacco plants. as a result, green fluorescent protein, which was produced in mesophyll and bundle sheath cells, could traffic into the sieve tube and trav ...200819704648
alternative sanitizers to chlorine for use on fresh-cut "galia" (cucumis melo var. catalupensis) melon.chlorine is commonly used to reduce microbial load in fresh-cut vegetables. however, the production of chlorinated organic compounds, such as trihalomethanes, which are potential carcinogens, has created the need to investigate the efficiency of nontraditional sanitizers and alternative techniques. the effects of 4 novel sanitizers were tested in fresh-cut "galia" melon: chlorine dioxide (clo(2)) at 3 mg/l, peracetic acid (paa) at 80 mg/l, hydrogen peroxide (h(2)o(2)) at 50 mg/l, and nisin at 25 ...200819021810
antisense suppression of an acid invertase gene (mai1) in muskmelon alters plant growth and fruit development.to unravel the roles of soluble acid invertase in muskmelon (cucumis melo l.), its activity in transgenic muskmelon plants was reduced by an antisense approach. for this purpose, a 1038 bp cdna fragment of muskmelon soluble acid invertase was expressed in antisense orientation behind the 35s promoter of the cauliflower mosaic virus. the phenotype of the antisense plants clearly differed from that of control plants. the transgenic plant leaves were markedly smaller, and the stems were obviously t ...200818641398
functional characterization of the arabidopsis atsuc2 sucrose/h+ symporter by tissue-specific complementation reveals an essential role in phloem loading but not in long-distance transport.atsuc2 (at1g22710) encodes a phloem-localized sucrose (suc)/h(+) symporter necessary for efficient suc transport from source tissues to sink tissues in arabidopsis (arabidopsis thaliana). atsuc2 is highly expressed in the collection phloem of mature leaves, and its function in phloem loading is well established. atsuc2, however, is also expressed strongly in the transport phloem, where its role is more ambiguous, and it has been implicated in mediating both efflux and retrieval to and from flank ...200818650401
the molecular and biochemical basis for varietal variation in sesquiterpene content in melon (cucumis melo l.) rinds.a combined chemical, biochemical and molecular study was conducted to understand the differential accumulation of volatile sesquiterpenes in melon fruits. sesquiterpenes were present mainly in the rinds of climacteric varieties, and a great diversity in their composition was found among varieties. sesquiterpenes were generally absent in non-climacteric varieties. two climacteric melon varieties, the green-fleshed 'noy yizre'el', and the orange-fleshed 'dulce' were further examined. in 'noy yizre ...200818264780
bin mapping of genomic and est-derived ssrs in melon (cucumis melo l.).we report the development of 158 primer pairs flanking ssr motifs in genomic (gssr) and est (est-ssr) melon sequences, all yielding polymorphic bands in melon germplasm, except one that was polymorphic only in cucurbita species. a similar polymorphism level was found among est-ssrs and gssrs, between dimeric and trimeric est-ssrs, and between est-ssrs placed in the open reading frame or any of the 5'- or 3'-untranslated regions. correlation between ssr length and polymorphism was only found for ...200818806992
identification of qtls for resistance to powdery mildew and ssr markers diagnostic for powdery mildew resistance genes in melon (cucumis melo l.).powdery mildew caused by podosphaera xanthii is an important foliar disease in melon. to find molecular markers for marker-assisted selection, we constructed a genetic linkage map of melon based on a population of 93 recombinant inbred lines derived from crosses between highly resistant ar 5 and susceptible 'earl's favourite (harukei 3)'. the map spans 877 cm and consists of 167 markers, comprising 157 simple sequence repeats (ssrs), 7 sequence characterized amplified region/cleavage amplified p ...200818797839
enzymatic detection of mercuric ions in ground-water from vegetable wastes by immobilizing pumpkin (cucumis melo) urease in calcium alginate beads.present report describes a quick and simple test based on enzyme inhibition for the detection of mercury in aqueous medium by urease immobilized in alginate beads. urease was extracted from the discarded seeds of pumpkin (cucumis melo) and was purified to apparent homogeneity (5.2-fold) by heat treatment at 48+/-0.1 degrees c and gel filtration through sephadex g-200. the homogeneous enzyme preparation (sp activity 353 u/mg protein, a(280)/a(260)=1.12) was immobilized in 3.5% alginate leading to ...200817933526
characterization and role of polyphenol oxidase and peroxidase in browning of fresh-cut melon.polyphenol oxidase (ppo) and peroxidase (pod) were extracted from two different varieties of melon ( cucumis melo l. cantalupensis cv. charentais and c. melo l. inodorus cv. amarillo) and characterized using reliable spectrophotometric methods. in both cases the enzymes followed michaelis-menten kinetics, showing different values of kinetics parameters between the two cultivars: k m = 7.18 +/- 0.70 mm ('charentais') and 6.66 +/- 0.20 mm ('amarillo') mm; v max = 7.93 +/- 0.35 units/min ('charenta ...200818069784
reme1, a copia retrotransposon in melon, is transcriptionally induced by uv light.for the first time, numerous sequences of copia and gypsy retrotransposons from the cucumis melo genome have been obtained and analyzed. phylogenetic analyses of sequences of both types of long terminal repeat (ltr) retrotransposons were carried out. the melon genome contains approximately 20,000 gypsy and 6,800 copia elements, comprising about 26% of its total size. starting from a retrotransposon fragment, we have cloned and characterized an entire melon retrotransposon, named reme1, which is ...200818034313
molecular authentication of three italian melon accessions by arms-pcr and its1 (internal transcribed spacer 1) secondary structure prediction.genetic assessment was carried out on three italian melon accessions by sequence and structural analysis of the internal transcribed spacer 1 (its1) from three populations belonging to two cucumis melo l. varieties (madras and tendral). alignment of the 18s-5.8s-26s sequences from three melon accessions showed that there were three single-nucleotide polymorphisms (snps) and one short insertion-deletion (indel) at the 5'end its1. an amplification refractory mutation system (arms)-pcr-based analys ...200818478086
factorial design for the optimization of enzymatic detection of cadmium in aqueous solution using immobilized urease from vegetable waste.free as well as alginate immobilized urease was utilized for detection and quantitation of cadmium (cd2+) in aqueous samples. urease from the seeds of pumpkin (cucumis melo), being a vegetable waste, was extracted and purified to apparent homogeneity (sp. activity 353 u/mg protein; a280/a260=1.12) by heat treatment at 48+/-0.1 degrees c and gel filtration through sephadex g-200. the homogeneous enzyme preparation was immobilized in 3.5% alginate leading to 86% immobilization and no leaching of t ...200818378444
melon fruits: genetic diversity, physiology, and biotechnology features.among cucurbitaceae, cucumis melo is one of the most important cultivated cucurbits. they are grown primarily for their fruit, which generally have a sweet aromatic flavor, with great diversity and size (50 g to 15 kg), flesh color (orange, green, white, and pink), rind color (green, yellow, white, orange, red, and gray), form (round, flat, and elongated), and dimension (4 to 200 cm). c. melo can be broken down into seven distinct types based on the previously discussed variations in the species ...200818322855
effect of gamma rays on different explants of callus treatment of multiple shoots in cucumis melo cv. bathasa.a mutagenesis programme was carried out using physical mutagens (gamma rays) on cucumis melo cv. bathasa. in irradiated seeds the number of shoots formed in the lower doses was significantly higher than the controls. decrease in the number of shoots and shoot bud formation was observed with higher doses in all the explants studies. there was complete lethality in the 10, 15 and 20 kr doses. in irradiated stem cultures the maximum number of shoots were observed in 2 kr. the number of shoots decre ...200819295084
employment of pumpkin (cucumis melo) urease entrapped in alginate beads for quantitation of cadmium in aqueous media.the potential of employment of free as well as alginate-immobilized urease for the quantitation of cadmium (cd(2+)) was explored. urease from the seeds of pumpkin (cucumis melo) was purified to apparent homogeneity by heat treatment at 48 +/- 0.1 degrees c and gel filtration through sephadex g-200. the purified enzyme exhibited a single band on native page under coomassie brilliant blue and silver staining. the enzyme entrapped in 3.5% alginate beads (with 86% immobilization) exhibited no leachi ...200818830881
determining nitrogen requirements of aceros and buceros hornbills.over 2 months, seven feeding trials were conducted at st. catherines island, ga, to quantify protein intake and utilization in captive mature nonreproducing aceros (n=3 spp.) and buceros (n=2 spp.) hornbills were fed homogeneous isocaloric diets. a mixture of bird of paradise pellets, grapes, and raisins was offered to the birds as grape-sized balls, supplemented with diced cantaloupe melon to maintain hydration. to vary the protein level (range 10.8-22.6%) within the diets, different amounts of ...200819360624
[effects of air temperature, solar radiation and soil water on dry matter accumulation and allocation of greenhouse muskmelon seedlings and related simulation models].with different sowing dates and irrigation upper limits, the effects of air temperature, solar radiation and soil water on the dry matter accumulation and allocation of greenhouse muskmelon seedlings were studied, with related simulation models established. the results showed that the dry matter accumulation and allocation of the seedlings had correlations with the changes of effective accumulative temperature, accumulative solar radiation, and irrigation upper limits at different seasons in a y ...200718333445
[effects of moisture and light intensity on ecophysiological characteristics of muskmelon seedlings].the muskmelon variety 'xiholuotuo' was used as a test material in order to study the combined effects of substrate moisture and light intensity on growth, development and ecophysiological characteristics of muskmelon seedings in greenhouse. the results showed that the different substrate moisture and light intensity remarkably influenced the growth and development of muskmelon seedlings. with reduction of substrate moisture, chl a, chl b and chl a + b increased, while relative water content (rwc ...200718260450
dielectric properties of honeydew melons and correlation with quality.dielectric properties of three honeydew melon cultivars, grown and harvested to provide a range of maturities, were measured with an open-ended coaxial-line probe and impedance analyzer over the frequency range from 10 mhz to 1.8 ghz. probe measurements were made on the external surface of the melons and also on tissue samples from the edible internal tissue. moisture content and soluble solids content (ssc) were measured for internal tissue samples, and ssc (sweetness) was used as the quality f ...200718161422
[effects of potassium level on photosynthetic characteristics and chloroplast submicroscopic structure of muskmelon leaves].with soilless culture in greenhouse, this paper studied the effects of three potassium (k) levels (120, 240, and 360 mg x l(-1)) on the photosynthetic characteristics and chloroplast submicroscopic structure of muskmelon cv. 'tiantian no. 1' leaves. the results showed that at low (120 mg x l(-1)) and high (360 mg x l(-1)) k levels, the net photosynthetic rate (pn) decreased, chloroplast lamellar structure disordered, and lamella number decreased. no significant effects were observed on co2 compe ...200717650859
correlating volatile compounds, sensory attributes, and quality parameters in stored fresh-cut cantaloupe.changes in post-cutting volatiles, quality, and sensory attributes during fresh-cut storage (4 degrees c) of cantaloupe (cucumis melo l. var. reticulatus, naudin, cv. 'sol real') harvested at four distinct maturities (1/4-, 1/2-, 3/4-, and full-slip) were investigated after 0, 2, 5, 7, 9, 12, and 14 days in a 2-year study. increased fruity and sweet taste attributes were negatively correlated with percent acetates, aromatic acetates, and total aromatic compounds, and positively correlated with p ...200717944532
herbal medicines as diuretics: a review of the scientific evidence.there is increasing interest in the health and wellness benefits of herbs and botanicals. this is with good reason as they might offer a natural safeguard against the development of certain conditions and be a putative treatment for some diseases. one such area may be the lowering of blood pressure in those where it is elevated (i.e., hypertension). one class of clinical medicines used to lower blood pressure are known as diuretics and work by increasing the excretion of urine from the body as w ...200717804183
melogen: an est database for melon functional genomics.melon (cucumis melo l.) is one of the most important fleshy fruits for fresh consumption. despite this, few genomic resources exist for this species. to facilitate the discovery of genes involved in essential traits, such as fruit development, fruit maturation and disease resistance, and to speed up the process of breeding new and better adapted melon varieties, we have produced a large collection of expressed sequence tags (ests) from eight normalized cdna libraries from different tissues in di ...200717767721
phylogenetics of cucumis (cucurbitaceae): cucumber (c. sativus) belongs in an asian/australian clade far from melon (c. melo).melon, cucumis melo, and cucumber, c. sativus, are among the most widely cultivated crops worldwide. cucumis, as traditionally conceived, is geographically centered in africa, with c. sativus and c. hystrix thought to be the only cucumis species in asia. this taxonomy forms the basis for all ongoing cucumis breeding and genomics efforts. we tested relationships among cucumis and related genera based on dna sequences from chloroplast gene, intron, and spacer regions (rbcl, matk, rpl20-rps12, trnl ...200717425784
detection of qtl for yield-related traits using recombinant inbred lines derived from exotic and elite us western shipping melon germplasm.the inheritance of yield-related traits in melon (cucumis melo l.; 2n = 2x = 24) is poorly understood, and the mapping of quantitative trait loci (qtl) for such traits has not been reported. therefore, a set of 81 recombinant inbred lines (ril) was developed from a cross between the monoecious, highly branched line usda 846-1 and a standard vining, andromonoecious cultivar, 'top mark'. the ril, parental lines, and three control cultivars ('esteem', 'sol dorado', and 'hales best jumbo') were grow ...200717294163
differential expression of the ascorbate oxidase multigene family during fruit development and in response to stress.ascorbate oxidase (ao, ec 1.10.3.3) is a member of the multicopper oxidases family. it catalyzes the oxidation of ascorbic acid (aa) to dehydroascorbic acid (dha) via monodehydroascorbate (mdha), with the concomitant reduction of molecular oxygen to water. in melon (cucumis melo), ascorbate oxidase is encoded by a multigene family comprising at least four genes. here, we present the detailed characterization of two melon ao genes, cmao1 and cmao4. gene-specific expression studies of the ao gene ...200717021803
gel electrophoresis of polyphenol oxidase with instant identification by in situ blotting.polyphenol oxidase (ppo) or tyrosinase is an important and ubiquitous enzyme responsible for browning in plants and melanization in animals. the molecular size of the plant ppo is varied among the species and its activity can be enhanced by a variety of anionic detergents. in the present study, we developed a simple method for the first-step identification of ppo in fruit and vegetable extracts. first, 3mm chromatographic paper was immersed in 0.5% (w/v) catechol solution as an immobilized ppo s ...200716973425
the cucurbits of mediterranean antiquity: identification of taxa from ancient images and descriptions.a critical analysis was made of cucurbit descriptions in dioscorides' de materia medica, columella's de re rustica and pliny's historia naturalis, works on medicine, agriculture and natural science of the 1st century ce, as well as the mishna and tosefta, compilations of rabbinic law derived from the same time period together with cucurbit images dating from antiquity including paintings, mosaics and sculpture. the goal was to identify taxonomically the mediterranean cucurbits at the time of the ...200717932073
[bee (apis mellifera, hymenoptera: apoidea) visitation to cantaloupe cucumis melo (cucurvitaceae) flowers in panama].flower visits by bees were observed in a melon cultivated field of san lorenzo district, in chiriqui, panama, from january 6 to february 19, 2002, from 6:30 am to 4:30 pm we recorded the duration of each foraging event and the type of resource collected. flower visits were mostly for nectar collection (approximately 75 %). pollen foraging was concentrated in the first hours of the morning and ended by 11:00 am the mean collection time was similar for both food resources, but was different betwee ...200719069779
mutator-like elements identified in melon, arabidopsis and rice contain ulp1 protease domains.the transposon mutator was first identified in maize, and is one of the most active mobile elements in plants. the arabidopsis thaliana genome contains at least 200 mutator-like elements (mules), which contain the mutator-like transposase gene, and often additional genes. we have detected a novel type of mules in melon (cumule), which, besides the transposase, contains two ubiquitin-like specific protease-like sequences (ulp1). this element is not present in the observed location in some melon c ...200717136348
an alternative sampling technique for cucumber beetles (coleoptera: chrysomelidae) and diurnal beetle activity on muskmelon.field studies were conducted to investigate the effectiveness of yellow sticky traps as an alternative sampling technique for striped, acalymma vittatum (f.), and spotted, diabrotica undecimpunctata howardi barber, cucumber beetles (coleoptera: chrysomelidae) and the diurnal beetle activity on muskmelon, cucumis melo l., near vincennes, in, in 2003 and 2004. the experimental design included six replications of seven 20-m-long rows each of muskmelon with 1.5 m between rows. on each sampling date, ...200717598544
characterization of a regional population of fusarium oxysporum f. sp. niveum by race, cross pathogenicity, and vegetative compatibility.abstract eighty-eight isolates of fusarium oxysporum f. sp. niveum, collected from wilted watermelon plants and infested soil in maryland and dela-ware, were characterized by cross pathogenicity to muskmelon, race, and vegetative compatibility. four isolates (4.5%) were moderately pathogenic to >/=2 of 18 muskmelon cultivars in a greenhouse test, and one representative isolate also was slightly pathogenic in field microplots. the four isolates all were designated as race 2, and were in vegetativ ...200718943287
monitoring the incidence and causes of diseases potentially transmitted by food in australia: annual report of the ozfoodnet network, 2006.in 2006, ozfoodnet sites reported 24,598 notifications of seven diseases or conditions that are commonly transmitted by food, representing an increase of 2.5% over the mean of the previous 5 years. the most frequently notified aetiological agents were campylobacter (15,492 notifications) and salmonella (8,331 notifications). salmonella notifications increased in 2006 by 5.2% when compared to historical reports. the most common salmonella serotype notified in australia during 2006 was salmonella ...200718268875
microbial survey of selected ontario-grown fresh fruits and vegetables.recent produce-related outbreaks have been receiving heightened media coverage, which has increased public concern toward the safety of fresh fruits and vegetables. in response, the microbial contamination of ontario-grown fresh fruits and vegetables was evaluated by the ontario ministry of agriculture, food and rural affairs during the summer of 2004. prior to this survey, information specific to the microbial contamination of ontario-produced fruits and vegetables was limited. this nonregulato ...200718095445
effect of hand wash agents on controlling the transmission of pathogenic bacteria from hands to food.the goals of this study were to evaluate the effectiveness of two hand wash regimens in reducing transient bacteria on the skin following a single hand wash and the subsequent transfer of the bacteria to a ready-to-eat food item, freshly cut cantaloupe melon. the number of bacteria recovered from hands and the quantity transferred to the melon were significantly less following the use of an antibacterial soap compared with plain soap. the antimicrobial soap achieved > 3-log reductions versus esc ...200718095447
induction of a small heat shock protein and its functional roles in nicotiana plants in the defense response against ralstonia solanacearum.in tobacco (nicotiana tabacum), ralstonia solanacearum oe1-1 (rsoe1-1) is pathogenic, whereas r. solanacearum 8107 (rs8107) is nonpathogenic and induces the hypersensitive response (hr). to elucidate the molecular mechanisms of plant-r. solanacearum interactions, we used differential display to isolate a cdna fragment, a6, regulated in tobacco by inoculation with rsoe1-1. the deduced amino acid sequence predicted from full-length a6-cdna showed similarity to small heat shock proteins from arabid ...200717965181
effect of time before storage and storage temperature on survival of salmonella inoculated on fresh-cut melons.the effects of a waiting period at room temperature ( approximately 22 degrees c) before refrigerating fresh-cut watermelon, cantaloupe and honeydew pieces contaminated with salmonella on survival of the inoculated pathogen were investigated. whole cantaloupes, honeydew melons and watermelons were washed with water, and fresh-cut pieces from individual melons were prepared and inoculated with a five strain cocktail of salmonella at 10(5)cfu/ml. populations of aerobic mesophilic bacteria, yeast a ...200717188207
effects of a cantaloupe melon extract/wheat gliadin biopolymer during aortic cross-clamping.we previously reported in healthy volunteers that a cantaloupe melon extract chemically combined with wheat gliadin (melon extract/gliadin) and containing sod, catalase and residual glutathione peroxidase (gpx), protected against dna strand-break damage induced by hyperbaric oxygen (hbo), a well-established model of dna damage resulting from oxidative stress. aortic cross-clamping is a typical example of ischemia/reperfusion injury-related oxidative stress, and therefore we investigated whether ...200717237934
ethylene regulation of fruit softening and cell wall disassembly in charentais melon.cell wall disassembly in ripening fruit is highly complex, involving the dismantling of multiple polysaccharide networks by diverse families of wall-modifying proteins. while it has been reported in several species that multiple members of each such family are expressed in the same fruit tissue, it is not clear whether this reflects functional redundancy, with protein isozymes from a single enzyme class performing similar roles and contributing equally to wall degradation, or whether they have d ...200717308329
one-step purification of histidine-tagged profilin with high purity and yield by using metal precipitation.a simple one-step method for the purification of recombinant his-tagged profilin from the bacterial cell lysate is reported. noting the greater ease with which hexahistidine-tagged proteins can be metalprecipitated compared with unwanted protein impurities, we investigated the effect of lysis-buffer additives and optimization of other conditions to recover selectively desired proteins in a one-step metal precipitation without using biopolymers. purification of the his-tagged melon (cucumis melo) ...200717313372
immunolocalization of a class iii chitinase in two muskmelon cultivars reacting differently to fusarium oxysporum f. sp. melonis.fusarium oxysporum f. sp. melonis is a highly specialized fungus that attacks the root system of melon (cucumis melo l.). in this work the presence of a class iii chitinase was examined by immunological techniques in the root and stem base of a susceptible (cv. galia) and a resistant (cv. bredor) melon during the infection process. by immunolocalization it was not possible to detect the constitutive presence of class iii chitinase in any of the cultivars. however, the immunolabelling appeared in ...200616360800
transformation of a muskmelon 'galia' hybrid parental line (cucumis melo l. var. reticulatus ser.) with an antisense acc oxidase gene.'galia' muskmelon (cucumis melo l. var. reticulatus ser.) has been recalcitrant to transformation by agrobacterium tumefaciens. transformation of the 'galia' male parental line, 'krymka', with an acc oxidase (cmaco-1) gene in antisense orientation is described herein. explants were transformed using a. tumefaciens strain abi, which contained a vector pcmaco1-as plasmid, bearing an antisense gene of cmaco-1 and the cp4 syn gene (glyphosate-tolerance). both cmaco-1 and cp4 syn genes were assessed ...200616362302
functional characterization of cmccd1, a carotenoid cleavage dioxygenase from melon.carotenoids are nutritionally important tetraterpenoid pigments that upon oxidative cleavage give rise to apocarotenoid (norisoprene) aroma volatiles. beta-carotene is the predominant pigment in orange-fleshed melon (cucumis melo l.) varieties, reaching levels of up to 50 microg/gfw. pale green and white cultivars have much lower levels (0-10 microg/gfw). in parallel, beta-ionone, the 9,10 cleavage product of beta-carotene, is present (12-33ng/gfw) in orange-fleshed melon varieties that accumula ...200616563447
pcr identification of salmonella: potential contamination sources from production and postharvest handling of cantaloupes.salmonella is one of the most frequently reported etiological agents in outbreaks of foodborne diseases associated with the consumption of cantaloupes. sensitive and reliable methods for detecting and identifying foodborne microorganisms are needed. the pcr can be used to amplify specific dna fragments and thus to detect and identify pathogenic bacteria. in this study, a pcr method was used to evaluate the incidence of salmonella at cantaloupe production, harvest, and packaging steps, and the re ...200616786866
cloning and expression analysis of a udp-galactose/glucose pyrophosphorylase from melon fruit provides evidence for the major metabolic pathway of galactose metabolism in raffinose oligosaccharide metabolizing plants.the cucurbitaceae translocate a significant portion of their photosynthate as raffinose and stachyose, which are galactosyl derivatives of sucrose. these are initially hydrolyzed by alpha-galactosidase to yield free galactose (gal) and, accordingly, gal metabolism is an important pathway in cucurbitaceae sink tissue. we report here on a novel plant-specific enzyme responsible for the nucleotide activation of phosphorylated gal and the subsequent entry of gal into sink metabolism. the enzyme was ...200616829585
two highly divergent alcohol dehydrogenases of melon exhibit fruit ripening-specific expression and distinct biochemical characteristics.alcohol dehydrogenases (adh) participate in the biosynthetic pathway of aroma volatiles in fruit by interconverting aldehydes to alcohols and providing substrates for the formation of esters. two highly divergent adh genes (15% identity at the amino acid level) of cantaloupe charentais melon (cucumis melo var. cantalupensis) have been isolated. cm-adh1 belongs to the medium-chain zinc-binding type of adhs and is highly similar to all adh genes expressed in fruit isolated so far. cm-adh2 belongs ...200616897483
hydroperoxide lyases (cyp74c and cyp74b) catalyze the homolytic isomerization of fatty acid hydroperoxides into hemiacetals.the conversion of linoleic acid 9-hydroperoxide (9-hpod) by recombinant melon (cucumis melo l.) hydroperoxide lyase (hpl, cyp74c subfamily) was studied. short (5 s-1 min) incubations at 0 degrees c followed by rapid extraction and trimethylsilylation made it possible to trap a new unstable (t(1/2) <30 s) product, i.e. the hemiacetal (1'e,3'z)-9-hydroxy-9-(1',3'-nonadienyloxy)-nonanoic acid. identification was performed by gc-ms analysis and substantiated by the formation of trimethylsilyl 9-trim ...200617049304
the wide distribution of endornaviruses, large double-stranded rna replicons with plasmid-like properties.the international committee on taxonomy of viruses (ictv) recently accepted endornavirus as a new genus of plant dsrna virus. we have determined the partial nucleotide sequences of the rna-dependent rna polymerase regions from the large dsrnas (about 14 kbp) isolated from barley (hordeum vulgare), kidney bean (phaseolus vulgaris), melon (cucumis melo), bottle gourd (lagenaria siceraria), malabar spinach (basella alba), seagrass (zostera marina), and the fungus helicobasidium mompa. phylogenetic ...200616341944
molecular phylogeny of cucumis species as revealed by consensus chloroplast ssr marker length and sequence variation.to investigate phylogenetic relationships in the genus cucumis, 9 consensus chloroplast simple sequence repeat (ccssr) primer pairs (ccssr3, 9, 11, 13, 14, 17, 20, 21, and 23) were employed for dna fragment length variation and 5 amplified fragments, ccssr4, 12, 13, 19, and 20, were sequenced using total dna from 13 accessions representing 7 african cucumis species (x = 12), 3 cucumis melo l. (x = 12) accessions, 2 cucumis sativus l. (x = 7) accessions, and 1 cucumis hystrix chakr. (x = 12) acce ...200616604104
the cucurbit images (1515-1518) of the villa farnesina, rome.the gorgeous frescoes organized by the master renaissance painter raphael sanzio (1483-1520) and illustrating the heavenly adventures of cupid and psyche were painted between 1515 and 1518 to decorate the roman villa (now known as the villa farnesina) of the wealthy sienese banker agostino chigi (1466-1520). surrounding these paintings are festoons of fruits, vegetables and flowers painted by giovanni martini da udine (1487-1564), which include over 170 species of plants. a deconstruction and co ...200616314340
comparative analysis of plant finds from early roman graves in ilok (cuccium) and sćitarjevo (andautonia), croatia--a contribution to understanding burial rites in southern pannonia.a comparative archaeobotanical analysis of the plant remains from the early roman incineration graves in ilok and sćitarjevo shows the existence of a complex burial ritual, but at the same time enables a better understanding of the agriculture and trade of the 1st/early 2nd century ad in southern pannonia. most of the cereals found (hordeum vulgare, panicum miliaceum, triticum monococcum, t. dicoccon, t. aestivum i t. cf. spelta), the legumes (lens culinaris, vicia ervilia) and the fruit contrib ...200616848163
subcellular localization and membrane topology of the melon ethylene receptor cmers1.ethylene receptors are multispanning membrane proteins that negatively regulate ethylene responses via the formation of a signaling complex with downstream elements. to better understand their biochemical functions, we investigated the membrane topology and subcellular localization of cmers1, a melon (cucumis melo) ethylene receptor that has three putative transmembrane domains at the n terminus. analyses using membrane fractionation and green fluorescent protein imaging approaches indicate that ...200616617090
purification and characterization of a serine protease from cucumis trigonus roxburghi.kachri fruit, cucumis trigonus roxburghi, contains high protease activity and has been used as meat tenderizer in the indian subcontinent. a 67 kda serine protease from kachri fruit was purified by deae-sepharose and cm-sepharose chromatography, whose optimum activity was at ph 11 and 70 degrees c. its activity was strongly inhibited by pmsf, but not by edta, pepstatin, or cysteine protease inhibitors. the substrate specificity of the purified protease towards synthetic peptides was comparable t ...200616603211
development of loop-mediated isothermal amplification (lamp)-based snp markers for shelf-life in melon (cucumis melo l.).in this study, lamp markers linked to shelf-life in melon (cucumis melo l.) were developed by converting a cleaved amplified polymorphic sequences (caps) marker (c2). the caps-pcr fragments from the long-shelf-life melon (o-3) and short-shelf-life melon (nat-2) were cloned and sequenced to construct lamp primers. a single nucleotide polymorphism (snp) was identified between o-3 and nat-2. lamp primers were designed to detect the snp. in the lamp reaction to detect long-shelf-life melon, the turb ...200617132894
[anthelmintic properties of cucumis melo seeds]. 200613294200
volatile compounds in the skin and pulp of queen anne's pocket melon.the quantitative distribution of volatile compounds in the skin and pulp of queen anne's pocket melon [cucumis melo var. dudaim (l.) naudin] has been investigated. volatile compounds were extracted by liquid-liquid microextraction (llme) using chloroform and analyzed by gc-fid and gc-ms. sixty volatiles, including 20 esters, 15 alcohols, 7 lactones, 7 aldehydes and ketones, 6 sulfur compounds, and 5 c(6) compounds, have been identified. among them, 38 were reported for the first time in pocket m ...200617032026
the melon fruit fly, bactrocera cucurbitae: a review of its biology and management.the melon fruit fly, bactrocera cucurbitae (coquillett) (diptera: tephritidae) is distributed widely in temperate, tropical, and sub-tropical regions of the world. it has been reported to damage 81 host plants and is a major pest of cucurbitaceous vegetables, particularly the bitter gourd (momordica charantia), muskmelon (cucumis melo), snap melon (c. melo var. momordica), and snake gourd (trichosanthes anguina). the extent of losses vary between 30 to 100%, depending on the cucurbit species and ...200517119622
development of a genomic library of near isogenic lines (nils) in melon (cucumis melo l.) from the exotic accession pi161375.a doubled haploid line (dhl) population of melon derived from a cross between the korean cultivar "songwhan charmi" accession pi161375 (sc), included in the horticultural group conomon, and the spanish cultivar "piel de sapo" (ps), included in the horticultural group inodorus, was used to develop a collection of near isogenic lines (nils). these parental lines represent very different melon cultivar groups, with important differences at fruit, plant, disease response and molecular level. this cr ...200516208502
analysis of the melon genome in regions encompassing tir-nbs-lrr resistance genes.plant genomes contain numerous genes (r-genes) that play a role in initiating defence measures against their particular pathogens. defence mechanisms controlled by r-genes have been the focus of extensive research over the past several years. the majority of the r-genes described so far belong to a super-family of genes (150-600 members) that encode proteins with a nucleotide binding site (nbs), some leucine-rich repeats (lrr) and an n-terminal domain that shows similarity to the toll and interl ...200515902490
simple-sequence repeat markers used in merging linkage maps of melon (cucumis melo l.).a set of 118 simple sequence repeat (ssr) markers has been developed in melon from two different sources: genomic libraries (gssr) and expressed sequence-tag (est) databases (est-ssr). forty-nine percent of the markers showed polymorphism between the 'piel de sapo' (ps) and pi161375 melon genotypes used as parents for the mapping populations. similar polymorphism levels were found in gssr (51.2%) and est-ssr (45.5%). two populations, f2 and a set of double haploid lines (dhls), developed from th ...200515700148
impact of melon accessions resistant to aphids on the demographic potential of silverleaf whitefly.bemisia tabaci (gennadius) and aphis gossypii glover are devastating melon, cucumis melo l., pests. the geographic areas where they occur overlap, and the same chemicals are used to control both of them. therefore, to reduce pesticide use, it would be necessary to breed melon lines that simultaneously express a resistance to both insects. female survival; the time when reproduction starts, peaks, and ends; the number of female offspring at the reproductive peak; and total reproduction (s) were d ...200515889749
use of cucumis metuliferus as a rootstock for melon to manage meloidogyne incognita.root-knot nematode-susceptible melons (cantaloupe) were grown in pots with varying levels of meloidogyne incognita and were compared to susceptible melons that were grafted onto cucumis metuliferus or cucurbita moschata rootstocks. in addition, the effect of using melons as transplants in nematode-infested soil was compared to direct seeding of melons in nematode-infested soil. there were no differences in shoot or root weight, or severity of root galling between transplanted and direct-seeded n ...200519262873
effects of application strategies of fumigant and nonfumigant nematicides on cantaloupe grown in deep sand soils in florida.a 2-year study was conducted in which three treatment tactics of oxamyl (at planting application, application every 2 weeks, and rescue applications, as determined by crop symptoms) were compared to fumigant treatments with methyl bromide, 1,3-dichloropropene (1,3-d), and 1,3-d plus chloropicrin for management of meloidogyne spp. in 2002, treatments that included 1,3-d produced higher yields as determined both by number and weight of marketable fruit. all treatment tactics relying solely on oxam ...200519262874
methylene blue staining method for identification of opisthorchis viverrini egg.methylene blue staining method was used to distinguish o. viverrini eggs from haplorchis taichui and prosthodendrium molenkampi eggs. all eggs were obtained from dissected adult worms, fixed in 10% formalin, and stained with methylene blue prior to light microscopy observation. the distinct musk-melon-like texture of the o. viverini eggshell surface and the thread-like texture of h. taichui eggshell surface were recognized, while p. molenkampi eggs showed a smooth eggshell. we also evaluated the ...200516438191
molecular markers linked to papaya ring spot virus resistance and fusarium race 2 resistance in melon.in melon, the fom-1 gene confers monogenic resistance against the soil-borne fungus fusarium oxysporum f. sp. melonis, races 0 and 2, while the closely linked prv gene specifies resistance against the papaya ring spot virus. markers linked to these resistance (r) genes were identified using two recombinant inbred line populations, derived from crosses between cucumis melo vedrantais and c. melo pi 161375, and between c. melo vedrantais and c. melo pi 414723, respectively. using bulked segregant ...200515551034
inheritance of resistance to watermelon mosaic virus in cucumis melo that impairs virus accumulation, symptom expression, and aphid transmission.abstract the cucumis melo accession tgr-1551 was found to be resistant to watermelon mosaic virus (wmv, genus potyvirus, family potyviridae). the resistance resulted in a drastic and significant reduction of virus titer and infected plants were asymptomatic or exhibited mild disease symptoms. the same gene or closely linked genes restricted virus accumulation and ameliorated symptom expression. no effect was observed on virus accumulation in inoculated leaves, which suggested that the initial ph ...200518943018
reducing salmonella on cantaloupes and honeydew melons using wash practices applicable to postharvest handling, foodservice, and consumer preparation.washing conditions that included a soak or brush scrub were evaluated for removal of salmonella from the surface of smooth (honeydew) or complex (cantaloupe) melon rinds. melon rinds were spot-inoculated onto a 2.5 cm2 area of rind (squares) with approximately 6.0 log(10) cfu/square of an avirulent nalidixic acid-resistant strain of salmonella typhimurium. melons were washed by immersion in 1500 ml of water or 200 ppm total chlorine and allowed to soak or were scrubbed over the entire melon surf ...200515718029
strain-specific and recessive qtls involved in the control of partial resistance to fusarium oxysporum f. sp. melonis race 1.2 in a recombinant inbred line population of melon.fusarium oxysporum f. sp. melonis (fom) causes serious economic losses in melon (cucumis melo l.). two dominant resistance genes have been identified, fom-1 and fom-2, which provide resistance to races 0 and 2 and races 0 and 1, respectively, however fom race 1.2 overcomes these resistance genes. a partial resistance to fom race 1.2 that has been found in some far east accessions is under polygenic control. a genetic map of melon was constructed to tag fom race 1.2 resistance with dna markers on ...200515834544
bacteremia caused by janibacter melonis.we report a case of bacteremia caused by janibacter melonis, a recently described aerobic actinomycete originally isolated from a spoiled oriental melon. our patient's blood culture isolate was identified by partial 16s rrna gene sequencing. this is the first report of the recovery of janibacter species from humans.200516000500
use of hydrogen peroxide in combination with nisin, sodium lactate and citric acid for reducing transfer of bacterial pathogens from whole melon surfaces to fresh-cut pieces.hydrogen peroxide (2.5%) alone or hydrogen peroxide (1%) in combination with nisin (25 microg/ml), sodium lactate (1%), and citric acid (0.5%) (hplnc) were investigated as potential sanitizers for reducing escherichia coli o157:h7 or listeria monocytogenes populations on whole cantaloupe and honeydew melons. whole cantaloupes inoculated with e. coli o157:h7 and l. monocytogenes at 5.27 and 4.07 log10 cfu/cm2, respectively, and whole honeydew melons inoculated with e. coli o157:h7 and l. monocyto ...200516043249
a physical map covering the nsv locus that confers resistance to melon necrotic spot virus in melon (cucumis melo l.).melon necrotic spot virus (mnsv) is a member of the genus carmovirus, which produces severe yield losses in melon and cucumber crops. the nsv gene is the only known natural source of resistance against mnsv in melon, and confers protection against all widespread strains of this virus. nsv has been previously mapped in melon linkage group 11, in a region spanning 5.9 cm, saturated with rapd and aflp markers. to identify the nsv gene by positional cloning, we started construction of a high-resolut ...200516052354
reduction of diabetes-induced renal oxidative stress by a cantaloupe melon extract/gliadin biopolymers, oxykine, in mice.oxidative stress is implicated as an important mechanism by which diabetes causes nephropathy. oxykine is the cantaloupe melon extract rich in vegetal superoxide dismutase covered by polymeric films of wheat matrix gliadin. in this study, we examined whether chronic oral administration of oxykine could prevent the progression of diabetic nephropathy induced by oxidative stress using preclinical rodent model of type 2 diabetes. we used female db/db mice and their non-diabetic db/m littermates. th ...200516179750
functional characterization of a melon alcohol acyl-transferase gene family involved in the biosynthesis of ester volatiles. identification of the crucial role of a threonine residue for enzyme activity*.volatile esters, a major class of compounds contributing to the aroma of many fruit, are synthesized by alcohol acyl-transferases (aat). we demonstrate here that, in charentais melon (cucumis melo var. cantalupensis), aat are encoded by a gene family of at least four members with amino acid identity ranging from 84% (cm-aat1/cm-aat2) and 58% (cm-aat1/cm-aat3) to only 22% (cm-aat1/cm-aat4). all encoded proteins, except cm-aat2, were enzymatically active upon expression in yeast and show different ...200516247561
atp bioluminescence assay for estimation of microbial populations of fresh-cut melon.estimation of microbial numbers in foods by conventional microbiological techniques takes days, so there is a need for faster methods that can give results in minutes. research was undertaken to investigate the use of bioluminescent atp determination and a firefly luciferase assay to estimate the initial population of aerobic mesophilic bacteria on fresh-cut melons immediately after preparation and during storage at 5 or 15 degrees c for up to 12 days. populations of aerobic mesophilic bacteria ...200516300083
the perisperm-endosperm envelope in cucumis: structure, proton diffusion and cell wall hydrolysing activity.and aims the envelope surrounding the embryo in cucurbit seed, which consists of a single layer of live endosperm cells covered by lipid- and callose-rich layers, is reported to show semi-permeability and also to act as the primary barrier to radicle emergence. structure, development and permeability of the envelope and activity of cell wall hydrolases during germination of cucumber and muskmelon seeds were investigated.200516043437
effect of hot water and hydrogen peroxide treatments on survival of salmonella and microbial quality of whole and fresh-cut cantaloupe.cantaloupe melon has been associated with outbreaks of salmonellosis. contamination might be introduced into the flesh from the rind by cutting or by contact of cut pieces with contaminated rinds. our objectives were to investigate the efficacy of hot water or hot 5% hydrogen peroxide treatments in reducing the population of native microflora and inoculated salmonella on cantaloupe rind and transfer to fresh-cut tissue during cutting. whole cantaloupes, inoculated with a cocktail of salmonella s ...200415035354
five independent loci each control monogenic resistance to gummy stem blight in melon ( cucumis melo l.).inheritance and segregation analysis demonstrated that five independent genes in melon confer monogenic resistance to foliar infection by the fungal pathogen didymella bryoniae, resulting in the disease known as gummy stem blight (gsb). in this study, two new monogenic sources of gsb resistance were characterized. resistance in cucumis melo pi 482398 was monogenic dominant based on segregation analysis of f(1), f(2) and backcross populations, while resistance in c. melo pi 482399 showed monogeni ...200415067389
phloem specific expression driven by wheat dwarf geminivirus v-sense promoter in transgenic dicotyledonous species.wheat dwarf geminivirus (wdv) is a single-stranded dna mastrevirus. the large intergenic region (lir) of wdv contains cis-acting elements essential for the replication of the genome as well as for the bidirectional transcription of virus genes. the lir was fused to the gus (uida) reporter gene and the wdv viral sense (v-sense) promoter activity derived from the stable integration of that promoter was analysed in transgenic dicot plants. various dicot species were tested, including nicotiana taba ...200415086824
molecular characterization of a melon necrotic spot virus strain that overcomes the resistance in melon and nonhost plants.resistance of melon (cucumis melo l.) to melon necrotic spot virus (mnsv) is inherited as a single recessive gene, denoted nsv. no mnsv isolates described to date (e.g., mnsv-malpha5), except for the mnsv-264 strain described here, are able to overcome the resistance conferred by nsv. analysis of protoplasts of susceptible (nsv/-) and resistant (nsv/nsv) melon cultivars inoculated with mnsv-264 or mnsv-malpha5 indicated that the resistance trait conferred by this gene is expressed at the single- ...200415195949
investigation of volatiles in charentais cantaloupe melons (cucumis melo var. cantalupensis). characterization of aroma constituents in some cultivars.volatile compounds of 15 charentais melon cultivars, known to exhibit differences in their ripening behaviors and in their storage lives (wild, mid, and long shelf life), were investigated. twenty-eight volatiles (11 esters, 8 sulfur compounds, 6 alcohols, and 3 carbonyl compounds) were isolated by direct dichloromethane extraction and analyzed by means of gc-ms and gc-fid. a considerable reduction in the aroma profile was observed for the long shelf life cultivars, in which total volatiles were ...200415237961
effect of hydrogen peroxide treatment on microbial quality and appearance of whole and fresh-cut melons contaminated with salmonella spp.the efficacy of hydrogen peroxide treatment on the inactivation of salmonella spp. inoculated on the external surface of cantaloupe and honeydew melon was investigated. salmonella was inoculated onto whole cantaloupe and honeydew melon to a final concentration of 4.65 log(10) cfu/cm(2) and 3.13 log(10) cfu/g, respectively. inoculated whole melons stored at 5 degrees c for up to 7 days were washed with water, 2.5% and 5% hydrogen peroxide at day 0 and 5. hydrogen peroxide (2.5% and 5%) treatments ...200415282126
optimizing concentration and timing of a phage spray application to reduce listeria monocytogenes on honeydew melon tissue.a phage cocktail was applied to honeydew melon pieces 1, 0.5, and 0 h before contamination with listeria monocytogenes strain lcdc 81-861 and 0.5, 1, 2, and 4 h after contamination. the phage application was most effective when applied 1, 0.5, or 0 h before contamination with l. monocytogenes, reducing pathogen populations by up to 6.8 log units after 7 days of storage. this indicates that under commercial conditions, if contamination occurs at the time of cutting, phage would have to be applied ...200415330534
comparative mapping of zymv resistances in cucumber (cucumis sativus l.) and melon (cucumis melo l.).zucchini yellow mosaic virus (zymv) routinely causes significant losses in cucumber ( cucumis sativus l.) and melon ( cucumis melo l.). zymv resistances from the cucumber population 'tmg1' and the melon plant introduction (pi) 414723 show different modes of inheritance and their genetic relationships are unknown. we used molecular markers tightly linked to zymv resistances from cucumber and melon for comparative mapping. a 5-kb genomic region (ycz-5) cosegregating with the zym locus of cucumber ...200415340688
janibacter melonis sp. nov., isolated from abnormally spoiled oriental melon in korea.two gram-positive bacterial strains, cm2104(t) and cm2110, isolated from the inner part of abnormally spoiled oriental melon (cucumis melo) in korea, were subjected to a polyphasic taxonomic study. the cell-wall peptidoglycan of strains cm2104(t) and cm2110 contained meso-diaminopimelic acid as the diagnostic diamino acid. the predominant menaquinone was mk-8(h(4)). the major fatty acids detected in the two strains were iso-c(16 : 0), c(17 : 1)omega8c and c(18 : 1)omega9c or c(17 : 0). the dna g ...200415545420
cerebroside elicitor confers resistance to fusarium disease in various plant species.abstract in the rice blast fungus pathosystem, cerebroside, a compound categorized as a sphingolipid, was found in our previous study to be a non-racespecific elicitor, which elicits defense responses in rice. here we describe that cerebroside c is produced in diverse strains of fusarium oxysporum, a common soilborne agent of wilt disease affecting a wide range of plant species. in addition, some type of cerebroside elicitor involving cerebroside a, b, or c was detected in other soilborne phytop ...200418943100
induced systemic resistance and promotion of plant growth by bacillus spp.abstract elicitation of induced systemic resistance (isr) by plant-associated bacteria was initially demonstrated using pseudomonas spp. and other gram-negative bacteria. several reviews have summarized various aspects of the large volume of literature on pseudomonas spp. as elicitors of isr. fewer published accounts of isr by bacillus spp. are available, and we review this literature for the first time. published results are summarized showing that specific strains of the species b. amyloliquef ...200418944464
[effects of exogenous gamma-aminobutyric acid on antioxidant enzyme activity and reactive oxygen content in muskmelon seedlings under nutrient solution hypoxia stress].the effects of exogenous gamma-aminobutyric acid (gaba) on antioxidant defense system and reactive oxygen species (ros) in seedlings of two varieties with different resistance to hypoxia stress were studied under hypoxia stress in a nutrient solution culture system. the results showed that gaba treatments enhanced the activities of antioxidant enzyme including sod (fig. 1), pod (fig. 2), cat (fig. 3), while the concentrations of h2o2 (fig. 4), o(-.)2 (fig. 5), mda (fig. 6) decreased under hypoxi ...200415643085
identification of quantitative trait loci involved in fruit quality traits in melon (cucumis melo l.).two populations [an f(2) and a set of 77 double haploid lines (dhls)] developed from a cross between a 'piel de sapo' cultivar (ps) and the exotic korean accession pi 161375 were used to detect qtls involved in melon fruit quality traits: earliness (ea), fruit shape (fs), fruit weight (fw) and sugar content (ssc); and loci involved in the colour traits: external colour (ecol) and flesh colour (fc). high variation was found, showing transgressive segregations for all traits. the highest correlati ...200414576985
antioxidant and anti-inflammatory properties of a cucumis melo lc. extract rich in superoxide dismutase activity.the present study was conducted to evaluate in vitro and in vivo the antioxidant and anti-inflammatory properties of a cantaloupe melon (cucumis melo lc., cucurbitaceae) extract (cme) selected for its high superoxide dismutase activity. peritoneal macrophages were pre-activated in vitro with 300 iu of interferon-gamma (ifn-gamma) and were then challenged in culture with iggl/anti-igg1 immune complexes (igg1ic) in presence of various cme extracts. the subsequent production of free radicals (super ...200415261965
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