Publications

differential transcriptional regulation of l-ascorbic acid content in peel and pulp of citrus fruits during development and maturation.	citrus fruits are an important source of ascorbic acid (asa) for human nutrition, but the main pathways involved in its biosynthesis and their regulation are still not fully characterized. to study the transcriptional regulation of asa accumulation, expression levels of 13 genes involved in asa biosynthesis, 5 in recycling and 5 in degradation were analyzed in peel and pulp of fruit of two varieties with different asa concentration: navel orange (citrus sinensis) and satsuma mandarin (citrus uns ...	2014	24567029
isolation, classification and transcription profiles of the ap2/erf transcription factor superfamily in citrus.	the ap2/erf gene family encodes plant-specific transcription factors. in model plants, ap2/erf genes have been shown to be expressed in response to developmental and environmental stimuli, and many function downstream of the ethylene, biotic, and abiotic stress signaling pathways. in citrus, ethylene is effective in regulation citrus fruit quality, such as degreening and aroma. however, information about the citrus ap2/erf family is limited, and would enhance our understanding of fruit responses ...	2014	24566692
characterization of mango (mangifera indica l.) transcriptome and chloroplast genome.	we characterized mango leaf transcriptome and chloroplast genome using next generation dna sequencing. the rna-seq output of mango transcriptome generated >12 million reads (total nucleotides sequenced >1 gb). de novo transcriptome assembly generated 30,509 unigenes with lengths in the range of 300 to ≥3,000 nt and 67× depth of coverage. blast searching against nonredundant nucleotide databases and several viridiplantae genomic datasets annotated 24,593 mango unigenes (80% of total) and identifi ...	2014	24515595
comparative analysis of surface wax in mature fruits between satsuma mandarin (citrus unshiu) and 'newhall' navel orange (citrus sinensis) from the perspective of crystal morphology, chemical composition and key gene expression.	surface wax of mature satsuma mandarin (citrus unshiu) and 'newhall' navel orange (citrus sinensis) was analysed by crystal morphology, chemical composition, and gene expression levels. the epicuticular and total waxes of both citrus cultivars were mostly composed of aldehydes, alkanes, fatty acids and primary alcohols. the epicuticular wax accounted for 80% of the total wax in the newhall fruits and was higher than that in the satsuma fruits. scanning electron microscopy showed that larger and ...	2014	24491718
characterization of the glutathione s-transferase gene family through ests and expression analyses within common and pigmented cultivars of citrus sinensis (l.) osbeck.	glutathione s-transferases (gsts) represent a ubiquitous gene family encoding detoxification enzymes able to recognize reactive electrophilic xenobiotic molecules as well as compounds of endogenous origin. anthocyanin pigments require gsts for their transport into the vacuole since their cytoplasmic retention is toxic to the cell. anthocyanin accumulation in citrus sinensis (l.) osbeck fruit flesh determines different phenotypes affecting the typical pigmentation of sicilian blood oranges. in th ...	2014	24490620
citrus sinensis annotation project (cap): a comprehensive database for sweet orange genome.	citrus is one of the most important and widely grown fruit crop with global production ranking firstly among all the fruit crops in the world. sweet orange accounts for more than half of the citrus production both in fresh fruit and processed juice. we have sequenced the draft genome of a double-haploid sweet orange (c. sinensis cv. valencia), and constructed the citrus sinensis annotation project (cap) to store and visualize the sequenced genomic and transcriptome data. cap provides gbrowse-bas ...	2014	24489955
determination of allergenic hydroperoxides in essential oils using gas chromatography with electron ionization mass spectrometry.	fragrance monoterpenes are widely used commercially due to their pleasant scent. in previous studies, we have shown that air-exposed monoterpenes form hydroperoxides that are strong skin sensitizers. methods for detection and quantification of the hydroperoxides in essential oils and scented products are thus desirable. due to thermolability and low uv absorbance, this is a complicated task. we have recently developed a sensitive lc-esi-ms method, but with limited structural information and sepa ...	2014	24482420
comparative de novo transcriptome analysis and metabolic pathway studies of citrus paradisi flavedo from naive stage to ripened stage.	grapefruit (citrus pardisi) is a popular citrus fruit that is a cross between a sweet orange and pummelo. this research article focuses on an in silico approach for comparative analysis of c. paradisi green flavedo (gf) and ethylene treated flavedo (etf) transcriptome data. our pathway analysis provides comprehensive information of genes playing significant role in different stages of ripening in fruit. de novo assembly was carried out using six different assemblers namely gs assembler, seqman n ...	2014	24477585
population of endogenous pararetrovirus genomes in carrizo citrange.	the complete genome sequences of three related endogenous pararetroviruses (eprvs) were obtained by 454 sequencing of nucleic acid extracts from carrizo citrange, used as a citrus rootstock. numerous homologous sequences have been found in the sweet orange genome. the new eprvs are most closely related to petunia vein-clearing virus.	2014	24435856
effect of sweet orange fruit waste diets and acidifier on haematology and serum chemistry of weanling rabbits.	a total of thirty-five mixed breed (35) rabbits of average weight of 700 g aged 5-6 weeks were allocated to seven treatments in a completely randomised design to investigate the effect of sweet orange fruit waste (sofw) and acidomix acidifier on haematology and serum chemistry. the diets were 0% sofw, 10% sofw with 0.5% acidomix, 10% sofw with 0.7 acidomix, 15% sofw with 0.5% acidifier, 15% sofw with 0.7% acidifier, 20% sofw with 0.5% acidifier, and 20% sofw with 0.7% acidifier. blood samples we ...	2014	26464931
genome-wide classification and evolutionary and expression analyses of citrus myb transcription factor families in sweet orange.	myb family genes are widely distributed in plants and comprise one of the largest transcription factors involved in various developmental processes and defense responses of plants. to date, few myb genes and little expression profiling have been reported for citrus. here, we describe and classify 177 members of the sweet orange myb gene (csmyb) family in terms of their genomic gene structures and similarity to their putative arabidopsis orthologs. according to these analyses, these csmybs were c ...	2014	25375352
cdna-aflp analysis reveals the adaptive responses of citrus to long-term boron-toxicity.	boron (b)-toxicity is an important disorder in agricultural regions across the world. seedlings of 'sour pummelo' (citrus grandis) and 'xuegan' (citrus sinensis) were fertigated every other day until drip with 10 μm (control) or 400 μm (b-toxic) h3bo3 in a complete nutrient solution for 15 weeks. the aims of this study were to elucidate the adaptive mechanisms of citrus plants to b-toxicity and to identify b-tolerant genes.	2014	25348611
involvements of pcd and changes in gene expression profile during self-pruning of spring shoots in sweet orange (citrus sinensis).	citrus shoot tips abscise at an anatomically distinct abscission zone (az) that separates the top part of the shoots into basal and apical portions (citrus self-pruning). cell separation occurs only at the az, which suggests its cells have distinctive molecular regulation. although several studies have looked into the morphological aspects of self-pruning process, the underlying molecular mechanisms remain unknown.	2014	25308090
genome-wide identification and phylogenetic analysis of the ap2/erf gene superfamily in sweet orange (citrus sinensis).	sweet orange (citrus sinensis) plays an important role in the economy of more than 140 countries, but it is grown in areas with intermittent stressful soil and climatic conditions. the stress tolerance could be addressed by manipulating the ethylene response factor (erf) transcription factors because they orchestrate plant responses to environmental stress. we performed an in silico study on the erfs in the expressed sequence tag database of c. sinensis to identify potential genes that regulate ...	2014	25299098
evaluation of the anti-proliferative and cytostatic effect of citrus sinensis (orange) fruit juice.	this work has been designed to evaluate the anti-proliferative and cytostatic effects of citrus sinensis (orange) fruit juice on rapidly proliferating cells.	2014	25298937
phenology of asian citrus psyllid (hemiptera: liviidae) and associated parasitoids on two species of citrus, kinnow mandarin and sweet orange, in punjab pakistan.	the population phenology of asian citrus psyllid, diaphorina citri kuwayama, was monitored weekly for 110 wk on two species of citrus, kinnow mandarin and sweet orange, at two different research sites in faisalabad, punjab pakistan. citrus flush growth patterns were monitored and natural enemy surveys were conducted weekly. flush patterns were similar for kinnow and sweet orange. however, flush on sweet orange was consistently more heavily infested with asian citrus psyllid than kinnow flush; de ...	2014	25198345
genome wide characterization of short tandem repeat markers in sweet orange (citrus sinensis).	sweet orange (citrus sinensis) is one of the major cultivated and most-consumed citrus species. with the goal of enhancing the genomic resources in citrus, we surveyed, developed and characterized microsatellite markers in the ≈347 mb sequence assembly of the sweet orange genome. a total of 50,846 ssrs were identified with a frequency of 146.4 ssrs/mbp. dinucleotide repeats are the most frequent repeat class and the highest density of ssrs was found in chromosome 4. ssrs are non-randomly distrib ...	2014	25148383
genome-wide comparison of micrornas and their targeted transcripts among leaf, flower and fruit of sweet orange.	in plants, micrornas (mirnas) regulate gene expression mainly at the post-transcriptional level. previous studies have demonstrated that mirna-mediated gene silencing pathways play vital roles in plant development. here, we used a high-throughput sequencing approach to characterize the mirnas and their targeted transcripts in the leaf, flower and fruit of sweet orange.	2014	25142253
comparative transcriptome analyses between a spontaneous late-ripening sweet orange mutant and its wild type suggest the functions of aba, sucrose and ja during citrus fruit ripening.	a spontaneous late-ripening mutant of 'jincheng' (c. sinensis l. osbeck) sweet orange exhibited a delay of fruit pigmentation and harvesting. in this work, we studied the processes of orange fruit ripening through the comparative analysis between the jincheng mutant and its wild type. this study revealed that the fruit quality began to differ on 166th days after anthesis. at this stage, fruits were subjected to transcriptome analysis by rna sequencing. 13,412 differentially expressed unigenes (d ...	2014	25551568
first evidence of c- and o-glycosyl flavone in blood orange (citrus sinensis (l.) osbeck) juice and their influence on antioxidant properties.	rp-lc-dad-esi-ms-ms separation/identification protocol has been employed for the identification and characterisation of nine c- and o-glycosyl flavonoids in moro (citrus sinensis (l.) osbeck) juice grown in southern italy. for the first time we reported the presence of five c-glycosyl flavones (lucenin-2, vicenin-2, stellarin-2, lucenin-2 4'-methyl ether and scoparin), a 3-hydroxy-3-methylglutaryl glycosyl flavonol (3-hydroxy-3-methylglutaryl glycosyl quercetin) and a flavone o-glycosides (chrys ...	2014	24295703
comprehensive insights on how 2,4-dichlorophenoxyacetic acid retards senescence in post-harvest citrus fruits using transcriptomic and proteomic approaches.	auxin-like 2,4-dichlorophenoxyacetic acid (2,4-d), a high-efficiency anti-stalling agent for the post-harvest fresh fruit industry, has had its use restricted due to environmental concerns. however, no other substitutes for 2,4-d are available to the post-harvest industry. insights into the molecular mechanism underlying the effects of 2,4-d on fruit quality preservation will provide a theoretical basis for exploring new safe and effective anti-stalling agents. this study comprehensively analyse ...	2014	24215076
metabolic engineering of β-carotene in orange fruit increases its in vivo antioxidant properties.	orange is a major crop and an important source of health-promoting bioactive compounds. increasing the levels of specific antioxidants in orange fruit through metabolic engineering could strengthen the fruit's health benefits. in this work, we have afforded enhancing the β-carotene content of orange fruit through blocking by rna interference the expression of an endogenous β-carotene hydroxylase gene (csβ-chx) that is involved in the conversion of β-carotene into xanthophylls. additionally, we h ...	2014	24034339
identification of genes associated with low furanocoumarin content in grapefruit.	some furanocoumarins in grapefruit (citrus paradisi) are associated with the so-called grapefruit juice effect. previous phytochemical quantification and genetic analysis suggested that the synthesis of these furanocoumarins may be controlled by a single gene in the pathway. in this study, cdna-amplified fragment length polymorphism (cdna-aflp) analysis of fruit tissues was performed to identify the candidate gene(s) likely associated with low furanocoumarin content in grapefruit. fifteen tentat ...	2014	25756876
phosphoproteomic analysis of chromoplasts from sweet orange during fruit ripening.	like other types of plastids, chromoplasts have essential biosynthetic and metabolic activities which may be regulated via post-translational modifications, such as phosphorylation, of their resident proteins. we here report a proteome-wide mapping of in vivo phosphorylation sites in chromoplast-enriched samples prepared from sweet orange [citrus sinensis (l.) osbeck] at different ripening stages by titanium dioxide-based affinity chromatography for phosphoprotein enrichment with lc-ms/ms. a tot ...	2014	23786612
metabolite signature of candidatus liberibacter asiaticus infection in two citrus varieties.	huanglongbing (hlb), also known as citrus greening disease, is caused by the bacterium 'candidatus liberibacter asiaticus' (clas) and is a serious threat to the citrus industry. to understand the effect of clas infection on the citrus metabolome, juice from healthy (n = 18), hlb-asymptomatic (n = 18), and hlb-symptomatic hamlin (n = 18), as well as from healthy (n = 18) and hlb-symptomatic (n = 18) valencia sweet oranges (from southern and eastern florida) were evaluated using (1)h nmr-based met ...	2014	24959841
metabolomic approaches for orange origin discrimination by ultra-high performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry.	in this work, hybrid quadrupole time-of-flight mass spectrometer (qtof ms) coupled to ultra high performance liquid chromatography (uhplc) has been used for biomarkers identification for correct authentication of valencia (spain) oranges. differentiation from foreign argentinean, brazilian and south african oranges has been carried out using xcms application and multivariate analysis to uhplc-(q)tof ms data acquired in both, positive and negative ionisation modes. several markers have been found ...	2014	24679755
comparison of carotenoid accumulation and biosynthetic gene expression between valencia and rohde red valencia sweet oranges.	carotenoid accumulation and biosynthetic gene expression levels during fruit maturation were compared between ordinary valencia (val) and its more deeply colored mutant rohde red valencia orange (rrv). the two cultivars exhibited different carotenoid profiles and regulatory mechanisms in flavedo and juice sacs, respectively. in flavedo, there was uncoordinated carotenoid accumulation and gene expression in rrv during green stages, which might be related to the expression of certain gene(s) in th ...	2014	25219303
[estimation of the tn and som contents in soil from gan nan navel orange plant area by nir diffuse spectroscopy].	the soil sampled from gan nan navel orange plant area was selected as research object, and the feasibility of analyzing the total nitrogen (tn) and soil organic matter (som) of soil was investigated by near infrared spectroscopy (nir) techniques in the wavelength range of 4 000 - 7 500 cm(-1). different pretreatment methods including multiplicative scatter correction (msc), first derivative(1st d), second derivative (2nd d), savitzkv-golay (sg), standard normalized variate (snv) and baseline wer ...	2013	24409716
modification of valencene by bio- and chemical transformation.	highly efficient production is discussed of nootkatone, the most important fragrant component of grapefruit and which possesses anti-obesity activity, from valencene, obtained from valencia orange, by the green alga, chlorella, and the fungi, mucor, botryospaeria and botryodiplodia. the microorganisms introduce an oxygen atom at an allylic position to give secondary hydroxyl and keto groups. the presented methods are a cheap one step reaction, non-hazardous and very useful for the production of ...	2013	23980411
behavior of parathion residues in the florida "valencia" orange agroecosystem.		2013	447929
distribution of radiocarbon in valencia oranges after treatment with 14 c-cycloheximide.		2013	5132643
the effect of home processing on postharvest fungicide residues in citrus fruit: residues of imazalil, 2-phenylphenol and thiabendazole in 'home-made' marmalade, prepared from late valencia oranges.	the effects of domestic marmalade-making procedures on residues of imazalil, 2-phenylphenol and thiabendazole in 'sweet' oranges were investigated by comparing residue levels in raw fruit and in marmalades, prepared by heating the oranges with water and sugar for about 4 h in a preserving pan on a gas ring or for about 1 h in a microwave oven. determination of residues in raw oranges and in marmalades was carried out by extraction with dichloromethane, followed by clean-up and reversed phase hpl ...	2013	8181633
effect of the use of a neutralizing step after antimicrobial application on microbial counts during challenge studies for orange disinfection.	the effects of using a neutralizer after applying antimicrobial treatments and the effect of time lapse between treatment application and subsequent recovery and enumeration of escherichia coli o157:h7 and salmonella were investigated in valencia oranges. inoculated oranges surfaces were washed with distilled water for 15 s and then sprayed with a solution containing 200 mg/liter sodium hypochlorite (ph 6.5) for 15 s; they were then dipped in l-lactic acid (2.0% at 55°c) for 1 min or in distille ...	2013	23433383
draft genome sequence of fructophilic lactobacillus florum.	herein we report the first genome sequence for lactobacillus florum. l. florum 2f was isolated from valencia orange leaves and is fructophilic, like other strains of this species. the draft genome of l. florum 2f contains 1,261,842 bp with a g+c content of 41.5% in 46 contigs (≥500 bp).	2013	23405360
stylet morphometrics and citrus leaf vein structure in relation to feeding behavior of the asian citrus psyllid diaphorina citri, vector of citrus huanglongbing bacterium.	the asian citrus psyllid (acp), diaphorina citri (hemiptera: psyllidae), is the primary vector of the phloem-limited bacterium candidatus liberibacter asiaticus (las) associated with huanglongbing (hlb, citrus greening), considered the world's most serious disease of citrus. stylet morphometrics of acp nymphs and adults were studied in relation to citrus vein structure and to their putative (histologically verified) feeding sites on valencia orange leaves. acp nymphs preferred to settle and feed ...	2013	23555830
digital gene expression analysis of corky split vein caused by boron deficiency in 'newhall' navel orange (citrus sinensis osbeck) for selecting differentially expressed genes related to vascular hypertrophy.	corky split vein caused by boron (b) deficiency in 'newhall' navel orange was studied in the present research. the boron-deficient citrus exhibited a symptom of corky split vein in mature leaves. morphologic and anatomical surveys at four representative phases of corky split veins showed that the symptom was the result of vascular hypertrophy. digital gene expression (dge) analysis was performed based on the illumina hiseq™ 2000 platform, which was applied to analyze the gene expression profilin ...	2013	23755275
genome-wide characterization and expression analysis of genetic variants in sweet orange.	heterozyosity is an important feature of many plant genomes, and is related to heterosis. sweet orange, a highly heterozygous species, is thought to have originated from an inter-species hybrid between pummelo and mandarin. to investigate the heterozygosity of the sweet orange genome and examine how this heterozygosity affects gene expression, we characterized the genome of valencia orange for single nucleotide variations (snvs), small insertions and deletions (indels) and structural variations ...	2013	23738603
red orange: experimental models and epidemiological evidence of its benefits on human health.	in recent years, there has been increasing public interest in plant antioxidants, thanks to the potential anticarcinogenic and cardioprotective actions mediated by their biochemical properties. the red (or blood) orange (citrus sinensis (l.) osbeck) is a pigmented sweet orange variety typical of eastern sicily (southern italy), california, and spain. in this paper, we discuss the main health-related properties of the red orange that include anticancer, anti-inflammatory, and cardiovascular prote ...	2013	23738032
the in vitro antimicrobial activity of lavandula angustifolia essential oil in combination with other aroma-therapeutic oils.	the antimicrobial activity of lavandula angustifolia essential oil was assessed in combination with 45 other oils to establish possible interactive properties. the composition of the selected essential oils was confirmed using gc-ms with a flame ionization detector. the microdilution minimum inhibitory concentration (mic) assay was undertaken, whereby the fractional inhibitory concentration (σfic) was calculated for the oil combinations. when lavender oil was assayed in 1 : 1 ratios with other o ...	2013	23737850
effects of citrus sinensis juice on blood pressure.	citrus sinensis juice (csj) is a rich source of dietary flavonoids which reduce the risk of adverse cardiovascular events. this study aimed to examine the effects of four-week intake of natural and commercial orange (citrus sinensis) juice on blood pressure in healthy volunteers.	2013	23696766
itraq protein profile analysis of citrus sinensis roots in response to long-term boron-deficiency.	seedlings of citrus sinensis were fertilized with boron (b)-deficient (0μm h3bo3) or -sufficient (10μm h3bo3) nutrient solution for 15weeks. thereafter, itraq analysis was employed to compare the abundances of proteins from b-deficient and -sufficient roots. in b-deficient roots, 164 up-regulated and 225 down-regulated proteins were identified. these proteins were grouped into the following functional categories: protein metabolism, nucleic acid metabolism, stress responses, carbohydrate and ene ...	2013	23628855
tropospheric ozone reduces carbon assimilation in trees: estimates from analysis of continuous flux measurements.	high ground-level ozone concentrations are typical of mediterranean climates. plant exposure to this oxidant is known to reduce carbon assimilation. ozone damage has been traditionally measured through manipulative experiments that do not consider long-term exposure and propagate large uncertainty by up-scaling leaf-level observations to ecosystem-level interpretations. we analyzed long-term continuous measurements (>9 site-years at 30 min resolution) of environmental and eco-physiological param ...	2013	23589473
fumigant toxicity of citrus sinensis essential oil on musca domestica l. adults in the absence and presence of a p450 inhibitor.	essential oils (eos) are potential tools for controlling musca domestica l. in a fumigant assay, m. domestica adults treated with citrus sinensis eo (lc50=3.9mg/dm(3)), with (4r)(+)-limonene (95.1%) being its main component, died within 15min or less. the terpenes absorbed by the flies and their metabolites, analyzed using spme fiber, were (4r)(+)-limonene (lc50=6.2mg/dm(3)), α-pinene (lc50=11.5mg/dm(3)), β-pinene (lc50=6.4mg/dm(3)), and two new components, carveol (lc50=1122mg/dm(3)) and carvon ...	2013	23545130
metabolic responses to iron deficiency in roots of carrizo citrange [citrus sinensis (l.) osbeck. x poncirus trifoliata (l.) raf].	the effects of iron (fe) deficiency on the low-molecular-weight organic acid (lmwoa) metabolism have been investigated in carrizo citrange (cc) [citrus sinensis (l.) osb. × poncirus trifoliata (l.) raf.] roots. major lmwoas found in roots, xylem sap and root exudates were citrate and malate and their concentrations increased with fe deficiency. the activities of several enzymes involved in the lmwoa metabolism were also assessed in roots. in the cytosolic fraction, the activities of malate dehyd ...	2013	23462311
modification of carotenoid levels by abscission agents and expression of carotenoid biosynthetic genes in 'valencia' sweet orange.	the effect of 5-chloro-3-methyl-4-nitro-1h-pyrazole (cmnp) and ethephon on peel color, flavedo carotenoid gene expression, and carotenoid accumulation was investigated in mature 'valencia' orange ( citrus sinensis l. osbeck) fruit flavedo at three maturation stages. abscission agent application altered peel color. cmnp was more effective than ethephon in promoting green-to-red (a) and blue-to-yellow (b) color at the middle and late maturation stages and total carotenoid changes at all maturation ...	2013	23451824
new excised-leaf assay method to test inoculativity of asian citrus psyllid (hemiptera: psyllidae) with candidatus liberibacter asiaticus associated with citrus huanglongbing disease.	the asian citrus psyllid, diaphorina citri kuwayama (hemiptera: psyllidae), is the primary vector of candidatus liberibacter asiaticus (las) associated with huanglongbing, or citrus greening, the most devastating citrus (citrus spp.) disease worldwide. here, we developed a new "excised-leaf assay" that can speed up las-inoculativity tests on asian citrus psyllid from the current 3-12 mo (when using whole citrus seedlings for inoculation) to only 2-3 wk. young adults of asian citrus psyllid that ...	2013	23448011
a sensitive method for determination of allergenic fragrance terpene hydroperoxides using liquid chromatography coupled with tandem mass spectrometry.	different compositions of monoterpenes are utilized for their pleasant scent in cosmetics and perfumes. however, the most commonly used fragrance terpenes easily oxidize upon contact with air, forming strongly skin-sensitizing hydroperoxides. due to their thermolability and low uv absorbance, detection methods for hydroperoxides are scarce. for the first time, a simple and sensitive method using lc/esi-ms/ms was developed to quantitatively determine hydroperoxides from the common fragrance compo ...	2013	23404959
the draft genome of sweet orange (citrus sinensis).	oranges are an important nutritional source for human health and have immense economic value. here we present a comprehensive analysis of the draft genome of sweet orange (citrus sinensis). the assembled sequence covers 87.3% of the estimated orange genome, which is relatively compact, as 20% is composed of repetitive elements. we predicted 29,445 protein-coding genes, half of which are in the heterozygous state. with additional sequencing of two more citrus species and comparative analyses of s ...	2013	23179022
metabolism in orange fruits is driven by photooxidative stress in the leaves.	in plants, stress signals propagate to trigger distant responses and thus stress acclimation in non-exposed organs. we tested here the hypothesis that leaves submitted to photooxidative stress may influence the metabolism of nearby fruits and thus quality criteria. leaves of orange trees (citrus sinensis (l.) osbeck cv. 'navelate') were acclimated to shade for 1 week and then submitted to full (fl) and medium light (ml) conditions. as expected, photoinhibition was detected in leaves of both fl a ...	2013	23330573
phytochemical profile and antioxidant activity of physiological drop of citrus fruits.	the phytochemical content and the antioxidant activity (aa) of physiological drop of the main citrus species grown in china were investigated. among the flavonoids, hesperidin was found mostly in mandarin and sweet orange, naringin was found mostly in sour orange, pummelo, grapefruit and a hybrid (gaocheng), narirutin was found in most varieties, neohesperidin was found in gaocheng and huyou, and nobiletin and tangeretin were found in most varieties. hydroxycinnamic acids were the main phenolic ...	2013	23301602
the influence of citrus rootstocks on the relationship between the mite brevipalpus phoenicis and citrus leprosis disease.	leprosis is one of the most serious citrus plant diseases. leprosis-affected plants, especially sweet orange [citrus sinensis (l.) osbeck], which is the most widely cultivated citrus fruit worldwide, show reduced photosynthetic capacity and severe defoliation. the aim was to evaluate the relationship between the brevipalpus phoenicis (geijskes) vector mite and citrus leprosis disease in pera sweet orange plants grafted on different rootstocks. data were analysed using numerical classification an ...	2013	22807324
[spider diversity in cultures of citrus sinensis (rutaceae) in corrientes province, argentina].	spiders are predators that reduce insect pest populations in agroecosystems. in spite that the presence of spider assemblages has been described in different crop plants, no reports have been done for citrus species in argentina. we studied the spider community associated with cultures of citrus sinensis in the province of corrientes, argentina, in two plots (am1 irrigated and am2 unirrigated) of 0.82 hectares each. spiders were collected monthly by using pitfall traps, hand capture, agitation o ...	2013	24027921
citrus nobiletin suppresses inducible nitric oxide synthase gene expression in interleukin-1β-treated hepatocytes.	nobiletin is a polymethoxylated flavone that is abundant in the peels of citrus fruits, such as citrus unshiu (satsuma mandarin) and citrus sinensis. the dried peels of c. unshiu (chinpi) have been included in several formulae of japanese kampo medicines. nobiletin may suppress the induction of inducible nitric oxide synthase (inos), which synthesizes the inflammatory mediator nitric oxide (no) in hepatocytes.	2013	23958298
species composition and abundance of brevipalpus spp. on different citrus species in mexican orchards.	we studied the abundance of brevipalpus spp. in citrus orchards in the mexican states of yucatan, quintana roo and campeche. mites were collected from 100 trees containing a mixture of citrus species where sweet orange was always the main species. eight collections were made at each location from february 2010 to february 2011. mites from the genus brevipalpus were separated from other mites surveyed and their abundance and relationships with the different citrus species were quantified througho ...	2013	23949863
the off-line combination of high performance liquid chromatography and comprehensive two-dimensional gas chromatography-mass spectrometry: a powerful approach for highly detailed essential oil analysis.	the present contribution is focused on the off-line combination of high performance liquid chromatography (hplc) and comprehensive two-dimensional gas chromatography-quadrupole mass spectrometry (gc×gc-quadms), and its application to the detailed qualitative analysis of essential oils. specifically, a silica column was exploited for the separation of the essential oil constituents in two groups, namely hydrocarbon and oxygenated compounds. after, each hplc-fraction was reduced in volume, and the ...	2013	23890548
the role of 1-deoxy-d-xylulose-5-phosphate synthase and phytoene synthase gene family in citrus carotenoid accumulation.	three 1-deoxy-d-xylulose-5-phosphate synthases (dxs) and three phytoene synthases (psy) were identified in citrus, from affymetrix genechip citrus genome array, genbank and public orange genome databases. tissue-specific expression analysis of these genes was carried out on fruit peel and flesh, flower and leaf of satsuma mandarin (citrus unshiu marc.) in order to determine their roles in carotenoid accumulation in different tissues. expression of citdxs1 and citpsy1 was highest in all test tiss ...	2013	23883976
a direct sensitivity comparison between flow-modulated comprehensive 2d and 1d gc in untargeted and targeted ms-based experiments.	the present contribution is focused on the measurement of the analytical sensitivity attained in untargeted/targeted ms/ms experiments, performed using flow-modulator comprehensive 2d and 1d gc. the comprehensive 2d experiment was performed by diverting part of the high flow (circa 80%) to flush the accumulation loop (about 28 ml/min) to waste, to reduce the gas flow entering the ion source. 1d analyses were performed through: (i) unmodulated and (ii) single column applications. an equivalent te ...	2013	23868497
interplay between aba and phospholipases a(2) and d in the response of citrus fruit to postharvest dehydration.	the interplay between abscisic acid (aba) and phospholipases a2 and d (pla2 and pld) in the response of citrus fruit to water stress was investigated during postharvest by using an aba-deficient mutant from 'navelate' orange named 'pinalate'. fruit from both varieties harvested at two different maturation stages (mature-green and full-mature) were subjected to prolonged water loss inducing stem-end rind breakdown (serb) in full-mature fruit. treatment with pla2 inhibitor aristolochic acid (at) a ...	2013	23800664
mining of haplotype-based expressed sequence tag single nucleotide polymorphisms in citrus.	single nucleotide polymorphisms (snps), the most abundant variations in a genome, have been widely used in various studies. detection and characterization of citrus haplotype-based expressed sequence tag (est) snps will greatly facilitate further utilization of these gene-based resources.	2013	24175923
coumarin and furanocoumarin quantitation in citrus peel via ultraperformance liquid chromatography coupled with mass spectrometry (uplc-ms).	coumarins and furanocoumarins are secondary metabolites commonly found in citrus plants. these molecules are allelochemical compounds in plants that have controversial effects on humans, such as phototoxicity and the commonly described interactions with drugs, referred to as the "grapefruit juice effect". thus, it is important to develop a reliable method to identify and quantitate the coumarins and furanocoumarins in citrus extracts. for this purpose, we herein describe an ultraperformance liqu ...	2013	24117278
leaf cdna-aflp analysis of two citrus species differing in manganese tolerance in response to long-term manganese-toxicity.	very little is known about manganese (mn)-toxicity-responsive genes in citrus plants. seedlings of 'xuegan' (citrus sinensis) and 'sour pummelo' (citrus grandis) were irrigated for 17 weeks with nutrient solution containing 2 μm (control) or 600 μm (mn-toxicity) mnso₄. the objectives of this study were to understand the mechanisms of citrus mn-tolerance and to identify differentially expressed genes, which might be involved in mn-tolerance.	2013	24034812
citrange fruit extracts alleviate obesity-associated metabolic disorder in high-fat diet-induced obese c57bl/6 mouse.	obesity is becoming one of the global epidemics of the 21st century. in this study, the effects of citrange (citrus sinensis × poncirus trifoliata) fruit extracts in high-fat (hf) diet-induced obesity mice were studied. female c57bl/6 mice were fed respectively a chow diet (control), an hf diet, hf diet supplemented with 1% w/w citrange peel extract (cpe) or 1% w/w citrange flesh and seed extract (cfse) for 8 weeks. our results showed that both cpe and cfse regulated the glucose metabolic disord ...	2013	24317433
citrus phenylpropanoids and defence against pathogens. part ii: gene expression and metabolite accumulation in the response of fruits to penicillium digitatum infection.	the effect of infection of citrus sinensis (var. navelina) fruits with penicillium digitatum was studied at gene expression and metabolite levels. in this study, expression of genes involved in the phenylpropanoid pathway was studied in the flavedo (outer coloured part of the peel) and albedo (inner white part) in response to pathogen infection. results of the time-course experiment showed that maximal expression of 10 out of 17 phenylpropanoid genes analysed occurred at 48h post-inoculation, wh ...	2013	23017425
citrus maf1, a repressor of rna polymerase iii, binds the xanthomonas citri canker elicitor ptha4 and suppresses citrus canker development.	transcription activator-like (tal) effectors from xanthomonas species pathogens act as transcription factors in plant cells; however, how tal effectors activate host transcription is unknown. we found previously that tal effectors of the citrus canker pathogen xanthomonas citri, known as pthas, bind the carboxyl-terminal domain of the sweet orange (citrus sinensis) rna polymerase ii (pol ii) and inhibit the activity of cscyp, a cyclophilin associated with the carboxyl-terminal domain of the citr ...	2013	23898043
a redox 2-cys mechanism regulates the catalytic activity of divergent cyclophilins.	the citrus (citrus sinensis) cyclophilin cscyp is a target of the xanthomonas citri transcription activator-like effector ptha, required to elicit cankers on citrus. cscyp binds the citrus thioredoxin cstdx and the carboxyl-terminal domain of rna polymerase ii and is a divergent cyclophilin that carries the additional loop ksgkplh, invariable cysteine (cys) residues cys-40 and cys-168, and the conserved glutamate (glu) glu-83. despite the suggested roles in atp and metal binding, the functions o ...	2013	23709667
draft genome sequence of methylobacterium mesophilicum strain sr1.6/6, isolated from citrus sinensis.	methylobacterium mesophilicum strain sr1.6/6 is an endophytic bacterium isolated from a surface-sterilized citrus sinensis branch. ecological and biotechnological aspects of this bacterium, such as the genes involved in its association with the host plant and the primary oxidation of methanol, were annotated in the draft genome.	2013	23788544
computational predictions provide insights into the biology of tal effector target sites.	transcription activator-like (tal) effectors are injected into host plant cells by xanthomonas bacteria to function as transcriptional activators for the benefit of the pathogen. the dna binding domain of tal effectors is composed of conserved amino acid repeat structures containing repeat-variable diresidues (rvds) that determine dna binding specificity. in this paper, we present talgetter, a new approach for predicting tal effector target sites based on a statistical model. in contrast to prev ...	2013	23526890
terpene down-regulation triggers defense responses in transgenic orange leading to resistance against fungal pathogens.	terpenoid volatiles are isoprene compounds that are emitted by plants to communicate with the environment. in addition to their function in repelling herbivores and attracting carnivorous predators in green tissues, the presumed primary function of terpenoid volatiles released from mature fruits is the attraction of seed-dispersing animals. mature oranges (citrus sinensis) primarily accumulate terpenes in peel oil glands, with d-limonene accounting for approximately 97% of the total volatile ter ...	2013	24192451
an hplc-ms characterization of the changes in sweet orange leaf metabolite profile following infection by the bacterial pathogen candidatus liberibacter asiaticus.	huanglongbing (hlb) presumably caused by candidatus liberibacter asiaticus (clas) threatens the commercial u.s. citrus crop of an annual value of $3 billion. the earliest shift in metabolite profiles of leaves from greenhouse-grown sweet orange trees infected with clas, and of healthy leaves, was characterized by hplc-ms concurrently with pcr testing for the presence of clas bacteria and observation of disease symptoms. twenty, 8-month-old 'valencia' and 'hamlin' trees were grafted with budwood ...	2013	24223954
candidatus liberibacter americanus induces significant reprogramming of the transcriptome of the susceptible citrus genotype.	citrus huanglongbing (hlb) disease is caused by endogenous, phloem-restricted, gram negative, uncultured bacteria named candidatus liberibacter africanus (calaf), ca. l. asiaticus (calas), and ca. l. americanus (calam), depending on the continent where the bacteria were first detected. the asian citrus psyllid vector, diaphorina citri, transmits calas and calam and both liberibacter species are present in brazil. several studies of the transcriptional response of citrus plants manifesting hlb sy ...	2013	23586643
small rna profiling reveals phosphorus deficiency as a contributing factor in symptom expression for citrus huanglongbing disease.	huanglongbing (hlb) is a devastating citrus disease that is associated with bacteria of the genus 'candidatus liberibacter' (ca. l.). powerful diagnostic tools and management strategies are desired to control hlb. host small rnas (srna) play a vital role in regulating host responses to pathogen infection and are used as early diagnostic markers for many human diseases, including cancers. to determine whether citrus srnas regulate host responses to hlb, srnas were profiled from citrus sinensis 10 ...	2013	23292880
the genetic diversity of citrus dwarfing viroid populations is mainly dependent on the infected host species.	as with viruses, viroids infect their hosts as polymorphic populations of variants. identifying possible sources of genetic variability is significant in the case of the species citrus dwarfing viroid (cdvd) which has been proposed as a dwarfing agent for high-density citrus plantings. here, a natural cdvd isolate (cmc) was used as an inoculum source for long-term (25 years) and short-term (1 year) bioassays in different citrus host species. characterization of progenies indicated that the genet ...	2013	23152366
evidence of recombinant citrus tristeza virus isolate occurring in acid lime cv. pant lemon orchard in uttarakhand terai region of northern himalaya in india.	the present study for the first time describes biological and molecular characterization of citrus tristeza virus (ctv) occurring in the terai area of uttarakhand state in northern himalaya region of india. direct antigen coated-elisa and reverse transcriptase-polymerase chain reaction (rt-pcr) detected the ctv infection in acid lime cv. pant lemon (citrus aurantifolia) orchards of pantnagar with an estimated disease incidence of 16.6-20.5 %. to know the biological and genetic properties, an iso ...	2013	24426255
comparison of antifungal activities of vietnamese citrus essential oils.	citrus essential oils (eos) are volatile compounds from citrus peels and widely used in perfumes, cosmetics, soaps and aromatherapy. in this study, inhibition of citrus eos extracted from vietnamese orange (citrus sinensis), mandarin (citrus reticulata blanco), pomelo (citrus grandis osbeck) and lime (citrus aurantifolia swingle) on the growth of plant pathogenic fungi, mucor hiemalis, penicillium expansum and fusarium proliferatum was investigated. the eos of the citrus peels were obtained by c ...	2013	22799453
synergistic combinations of high hydrostatic pressure and essential oils or their constituents and their use in preservation of fruit juices.	this work addresses the inactivation achieved with escherichia coli o157:h7 and listeria monocytogenes egd-e by combined processes of high hydrostatic pressure (hhp) and essential oils (eos) or their chemical constituents (ccs). hhp treatments (175-400 mpa for 20 min) were combined with 200 μl/l of each eo (citrus sinensis l., citrus lemon l., citrus reticulata l., thymus algeriensis l., eucalyptus globulus l., rosmarinus officinalis l., mentha pulegium l., juniperus phoenicea l., and cyperus lo ...	2013	23246609
transgenic sweet orange plants expressing a dermaseptin coding sequence show reduced symptoms of citrus canker disease.	citrus canker provoked by xanthomonas axonopodis pv. citri is a bacterial disease causing severe losses in all citrus-producing areas around the world. xanthomonas infection is considered as an endemic disease in northeast and northwest argentina, affecting as much as 10% of commercial citrus plantations. there is not known natural resistance neither in orange varieties nor in rootstocks used for grafting of commercial cultivars. to introduce resistance to this disease, plants of pineapple sweet ...	2013	23896218
increased resistance against citrus canker mediated by a citrus mitogen-activated protein kinase.	mitogen-activated protein kinases (mapk) play crucial roles in plant immunity. we previously identified a citrus mapk (csmapk1) as a differentially expressed protein in response to infection by xanthomonas aurantifolii, a bacterium that causes citrus canker in mexican lime but a hypersensitive reaction in sweet oranges. here, we confirm that, in sweet orange, csmapk1 is rapidly and preferentially induced by x. aurantifolii relative to xanthomonas citri. to investigate the role of csmapk1 in citr ...	2013	23777433
variations in type iii effector repertoires, pathological phenotypes and host range of xanthomonas citri pv. citri pathotypes.	the mechanisms determining the host range of xanthomonas are still undeciphered, despite much interest in their potential roles in the evolution and emergence of plant pathogenic bacteria. xanthomonas citri pv. citri (xci) is an interesting model of host specialization because of its pathogenic variants: pathotype a strains infect a wide range of rutaceous species, whereas pathotype a*/a(w) strains have a host range restricted to mexican lime (citrus aurantifolia) and alemow (citrus macrophylla) ...	2013	23437976
identification of transcription factors potentially involved in the juvenile to adult phase transition in citrus.	the juvenile to adult transition (jat) in higher plants is required for them to reach reproductive competence. however, it is a poorly understood process in woody plants, where only a few genes have been definitely identified as being involved in this transition. this work aims at increasing our understanding of the mechanisms regulating the jat in citrus.	2013	24052558
first report of maconellicoccus hirsutus (green, 1908) (hemiptera: coccoidea: pseudococcidae) and the associated parasitoid anagyrus kamali moursi, 1948 (hymenoptera: encyrtidae), in brazil.	the pink hibiscus mealybug (phm), maconellicoccus hirsutus (green) (hemiptera: pseudococcidae) and the associated hymenopterous parasitoid, anagyrus kamali moursi, 1948 (hymenoptera: encyrtidae), are reported for the first time in brazil. specimens of the phm were collected on nine hosts plants, annona muricata l. (anonnaceae), glycine max (l.) merr. (fabaceae), centrolobium paraensis tul. (fabaceae), inga edulis mart. (fabaceae), hibiscus rosa-sinensis l. (malvaceae), psidium guajava l. (myrtac ...	2013	23917571
rna-seq analysis of citrus reticulata in the early stages of xylella fastidiosa infection reveals auxin-related genes as a defense response.	citrus variegated chlorosis (cvc), caused by xylella fastidiosa, is one the most important citrus diseases, and affects all varieties of sweet orange (citrus sinensis l. osb). on the other hand, among the citrus genus there are different sources of resistance against x. fastidiosa. for these species identifying these defense genes could be an important step towards obtaining sweet orange resistant varieties through breeding or genetic engineering. to assess these genes we made use of mandarin (c ...	2013	24090429
n-acetylcysteine in agriculture, a novel use for an old molecule: focus on controlling the plant-pathogen xylella fastidiosa.	xylella fastidiosa is a plant pathogen bacterium that causes diseases in many different crops. in citrus, it causes citrus variegated chlorosis (cvc). the mechanism of pathogenicity of this bacterium is associated with its capacity to colonize and form a biofilm in the xylem vessels of host plants, and there is not yet any method to directly reduce populations of this pathogen in the field. in this study, we investigated the inhibitory effect of n-acetylcysteine (nac), a cysteine analogue used m ...	2013	24009716
development and systematic validation of qpcr assays for rapid and reliable differentiation of xylella fastidiosa strains causing citrus variegated chlorosis.	the xylem-limited, gram-negative, fastidious plant bacterium xylella fastidiosa is the causal agent of citrus variegated chlorosis (cvc), a destructive disease affecting approximately half of the citrus plantations in the state of são paulo, brazil. the disease was recently found in central america and is threatening the multi-billion u.s. citrus industry. many strains of x. fastidiosa are pathogens or endophytes in various plants growing in the u.s., and some strains cross infect several host p ...	2013	23123161
structural characterization of the thermally tolerant pectin methylesterase purified from citrus sinensis fruit and its gene sequence.	despite the longstanding importance of the thermally tolerant pectin methylesterase (tt-pme) activity in citrus juice processing and product quality, the unequivocal identification of the protein and its corresponding gene has remained elusive. tt-pme was purified from sweet orange [ citrus sinensis (l.) osbeck] finisher pulp (8.0 mg/1.3 kg tissue) with an improved purification scheme that provided 20-fold increased enzyme yield over previous results. structural characterization of electrophoret ...	2013	24328246
characterization of citrus sinensis transcription factors closely associated with the non-host response to xanthomonas campestris pv. vesicatoria.	plants, when exposed to certain pathogens, may display a form of genotype-independent resistance, known as non-host response. in this study, the response of citrus sinensis (sweet orange) leaves to xanthomonas campestris pv. vesicatoria (xcv), a pepper and tomato pathogenic bacterium, was analyzed through biochemical assays and cdna microarray hybridization and compared with asiatic citrus canker infection caused by xanthomonas citri subsp. citri. citrus leaves exposed to the non-host bacterium ...	2013	23453188
evaluation of codon biology in citrus and poncirus trifoliata based on genomic features and frame corrected expressed sequence tags.	citrus, as one of the globally important fruit trees, has been an object of interest for understanding genetics and evolutionary process in fruit crops. meta-analyses of 19 citrus species, including 4 globally and economically important citrus sinensis, citrus clementina, citrus reticulata, and 1 citrus relative poncirus trifoliata, were performed. we observed that codons ending with a- or t- at the wobble position were preferred in contrast to c- or g- ending codons, indicating a close associat ...	2013	23315666
antioxidant potential of a polyherbal antimalarial as an indicator of its therapeutic value.	nefang is a polyherbal product composed of mangifera indica (bark and leaf), psidium guajava, carica papaya, cymbopogon citratus, citrus sinensis, and ocimum gratissimum (leaves), used for the treatment of malaria. compounds with antioxidant activity are believed to modulate plasmodial infection. antioxidant activity of the constituent aqueous plants extracts, in vitro, was evaluated using the 2,2-diphenyl-1-picrylhydrazyl (dpph), total phenolic content (tpc), and ferric reducing antioxidant pow ...	2013	24454347
[new directions of research related to chronic wound healing].	optimal nutrition, immunological state and psychological condition play an important role in the process of chronic wound healing. infections caused by pathogens resistant to commonly used antibiotics additionally complicate and disturb regeneration of wounds. as part of the treatment, modern wound dressings are used, for example designed on the basis of alginates, dextranomers, hydrogels, hydrofiber, polyurethanes foams, hydrocolloids and liquids for wound debridement such us 0.9% nacl, the pwe ...	2013	24377187
identification of flavonoids with trypsin inhibitory activity extracted from orange peel and green tea leaves.	orange peel (citrus sinensis) and green tea (camellia sinensis) leaves, rich sources of food flavonoids, were analyzed for their trypsin inhibitory potential. hesperetin, rutin and hesperidin from orange peel, and catechin from green tea leaves, were isolated and their chemical structures were analyzed. all four compounds were evaluated for their trypsin inhibitory potential.	2013	23124490
oral dose of citrus peel extracts promotes wound repair in diabetic rats.	diabetic patients wound healing is slower than the healthy individuals. three citrus peel extracts; lemon (citrus limon), grapes fruits (citrus paradise) and orange (citrus sinensis) promote wound healing in experimental animals. this study investigated the effect of oral treatment with citrus peel extracts on wound repair of the skin of diabetic rats. the extracts were estimated for vitamin c and total carotenoid contents prior to animal study. diabetes mellitus was induced in rats by intraperi ...	2013	24506007
postharvest control of western flower thrips (thysanoptera: thripidae) and california red scale (hemiptera: diaspididae) with ethyl formate and its impact on citrus fruit quality.	the postharvest control of arthropod pests is a challenge that the california citrus industry must overcome when exporting fruit overseas. currently, methyl bromide fumigation is used to control postharvest pests on exported citrus, but it may soon be unavailable because of use restrictions and cost of this health-hazard ozone-depleting chemical. ethyl formate is a natural plant volatile and possible alternative to methyl bromide in postharvest insect control. the objectives of this study were 1 ...	2013	24498732
host susceptibility of citrus cultivars to queensland fruit fly (diptera: tephritidae).	citrus crops are considered to be relatively poor hosts for queensland fruit fly, bactrocera tryoni (froggatt), as for other tephritid species. australian citrus growers and crop consultants have reported observable differences in susceptibility of different citrus cultivars under commercial growing conditions. in this study we conducted laboratory tests and field surveys to determine susceptibility to b. tryoni of six citrus cultivars [(eureka lemon (citrus limon (l.) osbeck); navel and valenci ...	2013	23786078
green synthesized silver nanoparticles for selective colorimetric sensing of hg2+ in aqueous solution at wide ph range.	in the present study, the syntheses of crystalline silver and gold nanoparticles (nps) has been demonstrated in a green and environmentally friendly approach using citrus fruit extracts (lemon, citrus limon (cl-1) and sweet orange, citrus limetta (cl-2)). in addition, potentially hazardous metal ion sensing properties of these nps in aqueous solution has been explored. cl-1 and cl-2 that predominantly contained citric and ascorbic acid, exhibited different reducing abilities towards silver and g ...	2013	23741735
green synthesis of gold nanoparticles using citrus fruits (citrus limon, citrus reticulata and citrus sinensis) aqueous extract and its characterization.	this study reports the biological synthesis of gold nanoparticles by the reduction of haucl(4) by using citrus fruits (citrus limon, citrus reticulata and citrus sinensis) juice extract as the reducing and stabilizing agent. a various shape and size of gold nanoparticles were formed when the ratio of the reactants were altered with respect to 1.0mm chloroauric acid solution. the gold nanoparticles obtained were characterized by uv-visible spectra, transmission electron microscopy (tem) and x-ray ...	2013	23211617
chemical composition and anti-inflammatory effects of essential oil from hallabong flower.	a number of essential oils derived from plants are claimed to have several medicinal functions, including anti-cancer and anti-inflammation effects. however, the chemical composition and biological activities of flower-derived components have not been sufficiently characterized. therefore, we investigated the composition of essential oils from hallabong flower [(citrus unshiu marcov × citrus sinensis osbeck) × citrus reticulata blanco] and their anti-inflammatory effects. hydro-distilled essenti ...	2013	27366141
first record of anastrepha serpentina (wiedemann) (diptera: tephritidae) in citrus in brazil.	anastrepha serpentina (wiedemann) is recorded for the first time in citrus (rutaceae) in brazil. specimens were obtained from sweet orange (citrus sinensis) sampled in the municipalities of belém and capitão poço, and from mandarin orange (citrus reticulata) from tomé-açu, state of pará, brazil.	2013	23939279
tetraploid rangpur lime rootstock increases drought tolerance via enhanced constitutive root abscisic acid production.	whole-genome duplication, or polyploidy, is common in many plant species and often leads to better adaptation to adverse environmental condition. however, little is known about the physiological and molecular determinants underlying adaptation. we examined the drought tolerance in diploid (2x) and autotetraploid (4x) clones of rangpur lime (citrus limonia) rootstocks grafted with 2x valencia delta sweet orange (citrus sinensis) scions, named v/2xrl and v/4xrl, respectively. physiological experim ...	2013	23050986
purification of phospholipase d from citrus callus tissue.	phospholipase d in extracts of soluble proteins from callus cultures derived from cotyledons of citrus sinensis (l.) osbeck is activated by ca2+ and anionic detergents and has a ph optimum of 6.5. the enzyme was purified 703-fold over the crude protein extract with a yield of 15% by ammonium sulfate precipitation, ion exchange chromatography, gel filtration, hydrophobic interaction chromatography, and preparative acrylamide gel electrophoresis. preparative electrophoresis was carried out using c ...	2013	3688883
the lov protein of xanthomonas citri subsp. citri plays a significant role in the counteraction of plant immune responses during citrus canker.	pathogens interaction with a host plant starts a set of immune responses that result in complex changes in gene expression and plant physiology. light is an important modulator of plant defense response and recent studies have evidenced the novel influence of this environmental stimulus in the virulence of several bacterial pathogens. xanthomonas citri subsp. citri is the bacterium responsible for citrus canker disease, which affects most citrus cultivars. the ability of this bacterium to coloni ...	2013	24260514
structural and physiological analyses of the alkanesulphonate-binding protein (ssua) of the citrus pathogen xanthomonas citri.	the uptake of sulphur-containing compounds plays a pivotal role in the physiology of bacteria that live in aerobic soils where organosulfur compounds such as sulphonates and sulphate esters represent more than 95% of the available sulphur. until now, no information has been available on the uptake of sulphonates by bacterial plant pathogens, particularly those of the xanthomonas genus, which encompasses several pathogenic species. in the present study, we characterised the alkanesulphonate uptak ...	2013	24282519