Publications

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thio derivatives of 2(5h)-furanone as inhibitors against bacillus subtilis biofilms.gram-positive bacteria cause a wide spectrum of infectious diseases, including nosocomial infections. while in the biofilm, bacteria exhibit increased resistance to antibiotics and the human immune system, causing difficulties in treatment. thus, the development of biofilm formation inhibitors is a great challenge in pharmacology. the gram-positive bacterium bacillus subtilis is widely used as a model organism for studying biofilm formation. here, we report on the effect of new synthesized 2(5h) ...201726085951
oral immunization against candidiasis using lactobacillus casei displaying enolase 1 from candida albicans.candidiasis is a common fungal infection that is prevalent in immunocompromised individuals. in this study, an oral vaccine against candida albicans was developed by using the molecular display approach. enolase 1 protein (eno1p) of c. albicans was expressed on the lactobacillus casei cell surface by using poly-gamma-glutamic acid synthetase complex a from bacillus subtilis as an anchoring protein. the eno1p-displaying l. casei cells were used to immunize mice, which were later challenged with a ...201725853077
screening and characterization of a thermostable lipase from marine streptomyces sp. strain w007.a screening method along with the combination of genome sequence of microorganism, pairwise alignment, and lipase classification was used to search the thermostable lipase. then, a potential thermostable lipase (named mas1) from marine streptomyces sp. strain w007 was expressed in pichia pastoris x-33, and the biochemical properties were characterized. lipase mas1 belongs to the subfamily i.7, and it has 38% identity to the well-characterized bacillus subtilis thermostable lipases in the subfami ...201725639796
studies on the production of alkaline α-amylase from bacillus subtilis cb-18.amylases are among the main enzymes used in food and other industries. they hydrolyse starch molecules into polymers composing glucose units. amylases have potential applications in a number of industrial processes including foods and pharmaceutical industries. alkaline α-amylase has the potential of hydrolysing starch under alkaline ph and is useful in the starch and textile industries and as an ingredient of detergents. amylases are produced from plants, however, microbial production processes ...201728068022
structure-based approach to alter the substrate specificity of bacillus subtilis aminopeptidase.aminopeptidases can selectively catalyze the cleavage of the n-terminal amino acid residues from peptides and proteins. bacillus subtilis aminopeptidase (bsap) is most active toward p-nitroanilides (pnas) derivatives of leu, arg, and lys. the bsap with broad substrate specificity is expected to improve its application. based on an analysis of the predicted structure of bsap, four residues (leu 370, asn 385, ile 387, and val 396) located in the substrate binding region were selected for saturatio ...201723787698
potential application of bacillus subtilis spb1 lipopeptides in toothpaste formulation.toothpaste is a gel dentifrice used with a toothbrush as an accessory to clean, keep and promote oral hygiene. the literature review suggests that there are many different formulations of toothpastes and that each of their individual components present specific functions. the concentration of the toothpaste ingredients must be appropriately chosen taking into account the purposes of the toothpaste. biosurfactants are considered as suitable molecules for application in many formulations such as i ...201728721297
first report of two complete clostridium chauvoei genome sequences and detailed in silico genome analysis.clostridium (c.) chauvoei is a gram-positive, spore forming, anaerobic bacterium. it causes black leg in ruminants, a typically fatal histotoxic myonecrosis. high quality circular genome sequences were generated for the c. chauvoei type strain dsm 7528(t) (atcc 10092(t)) and a field strain 12s0467 isolated in germany. the origin of replication (oric) was comparable to that of bacillus subtilis in structure with two regions containing dnaa boxes. similar prophages were identified in the genomes o ...201728720440
sequence-based identification of inositol monophosphatase-like histidinol-phosphate phosphatases (hisn) in corynebacterium glutamicum, actinobacteria, and beyond.the eighth step of l-histidine biosynthesis is carried out by an enzyme called histidinol-phosphate phosphatase (holpase). three unrelated holpase families are known so far. two of them are well studied: had-type holpases known from gammaproteobacteria like escherichia coli or salmonella enterica and php-type holpases known from yeast and firmicutes like bacillus subtilis. however, the third family of holpases, the inositol monophosphatase (impase)-like holpases, present in actinobacteria like c ...201728720084
sequence comparison of phor, gyrb, groel, and chea genes as phylogenetic markers for distinguishing bacillus amyloliquefaciens and b. subtilis and for identifying bacillus strain b29.given the close genetic relationship between bacillus amyloliquefaciens and b. subtilis, distinguishing the two solely based on their physiological and biochemical characteristics and 16s rrna sequences is difficult. molecular identification was used to discover suitable genes for distinguishing the two bacteria, and to identify the bio-controlling strain b29, due to molecular identification has been paid more and more attention. the similarity of four genes, chea, gyrb, groel and phor, of the t ...201728719340
akanthopyrones a-d, α-pyrones bearing a 4-o-methyl-β-d-glucopyranose moiety from the spider-associated ascomycete akanthomyces novoguineensis.hypocrealean fungi have proved to be prolific bioactive metabolite producers; they have caught the attention of mycologists throughout the world. however, only a few studies on the insect and spider parasitic genus akanthomyces have so far been carried out. in this study, we report the isolation, structural elucidation and biological activities of four unprecedented glycosylated α-pyrone derivatives, akanthopyrones a-d (1-4), from a culture of akanthomyces novoguineensis collected in thailand. t ...201728718819
analysis of chemical signatures of alkaliphiles using fatty acid methyl ester analysis.fatty acids occur in nearly all living organisms as the important predominant constituents of lipids. while all fatty acids have essentially the same chemical nature, they are an extremely diverse group of compounds.201728717333
skin permeability enhancement by bacillus subtilis alkaline protease: application to transdermal drug delivery.enzymes may offer great potentials in topical pharmaceutical applications provided that treatment conditions are controlled for efficacy and safety. in this study, the effect of alkaline protease produced by recombinant bacillus subtilis cells on the ex-vivo permeability of rabbit ear skin was investigated under different conditions of enzyme activity (5-60 units) and exposure time (15-60min). data for transepidermal water loss (tewl) and permeation of a hydrophilic dye, rhodamine b (rb), indica ...201728711639
specificity of subtilin-mediated activation of histidine kinase spak.autoinduction via two-component systems is a widespread regulatory mechanism that senses environmental and metabolic changes. although the lantibiotics nisin and subtilin are closely related and share the same lanthionine ring structure, they autoinduce their biosynthesis in a highly specific manner. subtilin can only activate the two-component system spark of bacillus subtilis, whereas nisin activates solely the two-component system nisrk of lactococcus lactis to identify components that determ ...201728710266
protective effect of bacillopeptidase cfr5 from bacillus subtilis cfr5 on cerulein-induced pancreatitis.bacillopeptidase is a serine peptidase, known for its fibrinolytic activity. however, a very little information is known about its in vivo inflammatory and/or anti-inflammatory properties. thus, to understand whether bacillopeptidase incorporation can regulate pancreatitis or not, the cerulein-induced pancreatitis model was used, and the role of bacillopeptidase on pancreatitis was studied. in this study, 46 kda protein was purified from bacillus subtilis and identified as bacillopeptidase cfr5 ...201728709869
arsenic-resistant and plant growth promoting firmicutes and γ-proteobacteria species from industrially polluted irrigation water and corresponding cropland.the aim of the study was to explore irrigation water polluted with industrial waste and corresponding cropland to screen bacteria for as-detoxification and plant growth promotion.201728708308
investigation on the antimicrobial activities of gingers (etlingera coccinea (blume) s.sakai & nagam and etlingera sessilanthera r.m.sm.) endemic to borneo.to investigate the antimicrobial properties of e. coccinea and e. sessilanthera and to isolate and identify the antimicrobial compounds.201728708293
protein tagging, destruction and infection.cells possess protein quality control mechanisms to maintain proper cellular homeostasis. in eukaryotes, the roles of the ubiquitination and proteasome-mediated degradation of cellular proteins is well established. recent studies have elucidated protein tagging mechanisms in prokaryotes, involving transfer messenger rna (tmrna) and pupylation. in this review, newer insights and bioinformatics analysis of two distinct bacterial protein tagging machineries are discussed. the machinery for tmrna-me ...201728707598
crispr/cas9 editing of the bacillus subtilis genome.a fundamental procedure for most modern biologists is the genetic manipulation of the organism under study. although many different methods for editing bacterial genomes have been used in laboratories for decades, the adaptation of crispr/cas9 technology to bacterial genetics has allowed researchers to manipulate bacterial genomes with unparalleled facility. crispr/cas9 has allowed for genome edits to be more precise, while also increasing the efficiency of transferring mutations into a variety ...201728706963
synthesis of phytochemicals-stabilized gold nanoparticles and their biological activities against bacteria and leishmania.nanoscale materials have shown promising results in the field of medicine as therapeutic agents and drugs delivery vehicles. in the current study, gold nanoparticles (aunps) were prepared by a green and facile method using the aqueous extract of rhazya stricta decne as a source of reducing and stabilizing agents. the bio-fabricated aunps were characterized by uv-visible spectroscopy, x-ray diffraction (xrd), transmission electron microscopy (tem) and ftir spectroscopy. antimicrobial activities o ...201728705747
development and characterization of bioactive edible films from spider crab (maja crispata) chitosan incorporated with spirulina extract.active food packaging films based on crab chitosan and spirulina extract (se) were developed. the effects of the se incorporation at different levels on physical (color, opacity water vapor and oxygen permeability) and mechanical (tensile strength and elongation at break) properties of chitosan films were investigated. ftir was carried out to observe the potential modifications of the chitosan films when incorporated with se. the obtained results suggested that incorporation of se into chitosan ...201728705500
antimicrobial polyketide furanoterpenoids from seaweed-associated heterotrophic bacterium bacillus subtilis mtcc 10403.brown seaweed anthophycus longifolius (turner) kützing (family sargassaceae) associated heterotrophic bacterium bacillus subtilis mtcc 10403 was found to be a potent isolate with broad range of antibacterial activity against important perceptive food pathogens vibrio parahaemolyticus, v. vulnificus, and aeromonas hydrophila. this bacterium was positive for polyketide synthetase gene (kc589397), and therefore, was selected to bioprospect specialized metabolites bearing polyketide backbone. bioact ...201728704687
biological control of heterodera glycines by spore-forming plant growth-promoting rhizobacteria (pgpr) on soybean.heterodera glycines, the soybean cyst nematode, is the most economically important plant-parasitic nematode on soybean production in the u.s. the objectives of this study were to evaluate the potential of plant growth-promoting rhizobacteria (pgpr) strains for mortality of h. glycines j2 in vitro and for reducing nematode population density on soybean in greenhouse, microplot, and field trials. the major group causing mortality to h. glycines in vitro was the genus bacillus that consisted of 92. ...201728704498
identification, bioactivity, and productivity of actinomycins from the marine-derived streptomyces heliomycini.in the process of profiling the secondary metabolites of actinobacteria isolated from the saudi coastal habitats for production of antibiotics and anti-cancer drugs, the cultures of strain wh1 that was identified as streptomyces heliomycini exhibited strong antibacterial activity against staphylococcus aureus. by means of ms and nmr techniques, the active compounds were characterized as actinomycins x0β, x2, and d, respectively. the research on the productivity of this strain for actinomycins re ...201728702007
quality and characteristics of fermented ginseng seed oil based on bacterial strain and extraction method.in this study, the fermentation of ginseng seeds was hypothesized to produce useful physiologically-active substances, similar to that observed for fermented ginseng root. ginseng seed was fermented using bacillus, pediococcus, and lactobacillus strains to extract ginseng seed oil, and the extraction yield, color, and quantity of phenolic compounds, fatty acids, and phytosterol were then analyzed.201728701887
ligand recognition and helical stacking formation are intimately linked in the sam-i riboswitch regulatory mechanism.riboswitches are non-coding mrna elements that control gene expression by altering their structure upon metabolite binding. although riboswitch crystal structures provide detailed information about rna-ligand interactions, little knowledge has been gathered to understand how riboswitches modulate gene expression. here, we study the molecular recognition mechanism of the s-adenosylmethionine sam-i riboswitch by characterizing the formation of a helical stacking interaction involving the ligand bi ...201728701520
characterization of surface-active biofilm protein bsla in self-assembling langmuir monolayer at the air-water interface.biofilm is an extracellular matrix of bacteria and serves as a protective shield of bacterial communities. it is crucial for microbial growth and one of the leading causes of human chronic infections as well. however, the structures and molecular mechanism of biofilm formation remain largely unknown. here, we examined a protein, bsla, expressed in the biofilms of bacillus subtilis. we characterized the langmuir monolayers of bsla at the air/water interface. using techniques in surface chemistry ...201728701036
implementation of a loss-of-function system to determine growth and stress-associated mutagenesis in bacillus subtilis.a forward mutagenesis system based on the acquisition of mutations that inactivate the thymidylate synthase gene (tms) and confer a trimethoprim resistant (tmpr) phenotype was developed and utilized to study transcription-mediated mutagenesis (tmm). in addition to thya, bacillus subtilis possesses thyb, whose expression occurs under conditions of cell stress; therefore, we generated a thyb- thya+ mutant strain. tmpr colonies of this strain were produced with a spontaneous mutation frequency of ~ ...201728700593
antituberculotic activity of actinobacteria isolated from the rare habitats.a distinctive screening procedure resulted in the isolation and identification of antituberculotic actinobacteria. in this course, a total of 125 actinobacteria were isolated from various soil samples from untapped areas in northwestern himalayas, india. the antibacterial screening showed that 26 isolates inhibited the growth of at least one of the tested bacterial pathogens including staphylococcus aureus (atcc 25923), staphylococcus epidermidis (atcc 12228), bacillus subtilis (atcc 11774), mic ...201728699251
isolation and antibiotic screening of fungi from a hydrothermal vent site and characterization of secondary metabolites from a penicillium isolate.five new compounds were isolated from penicillium sp. y-5-2 including an austin derivative 4, four isocoumarins 9, 11, 12, and 13, together with two known isocoumarins 8 and 10, and six known austin derivatives 1, 2, 3, 5, 6, and 7 and one phenol 14. their structures and relative configurations were established by spectroscopic means. the absolute configurations of 4, 11, and 13 were defined mainly by comparison of quantum chemical tddft calculated and experimental ecd spectra. the cyclization o ...201728698959
antibacterial and antioxidant constituents of extracts of endophytic fungi isolated from ocimum basilicum var. thyrsiflora leaves.fourteen fungal endophytes were isolated from the ocimum basilicum var. thyrsiflora leaves collected from northern thailand. eight genera were identified including aspergillus, ascochyta, nigrospora, blastomyces, colletotrichum, exidia, clitopilus, and nomuraea. the antibacterial activity of crude extracts from all endophytic fungi was tested against nine human bacterial pathogens: staphylococcus aureus, staphylococcus epidermidis, bacillus subtilis, bacillus cereus, escherichia coli, klebsiella ...201728698913
the effect of silver nanoparticles size, produced using plant extract from arbutus unedo, on their antibacterial efficacy.silver nanoparticles (agnps) have been demonstrated to restrain bacterial growth, while maintaining minimal risk in development of bacterial resistance and human cell toxicity that conventional silver compounds exhibit. several physical and chemical methods have been reported to synthesize agnps. however, these methods are expensive and involve heavy chemical reduction agents. an alternative approach to produce agnps in a cost-effective and environmentally friendly way employs a biological pathw ...201728698511
bifunctionality of a biofilm matrix protein controlled by redox state.biofilms are communities of microbial cells that are encapsulated within a self-produced polymeric matrix. the matrix is critical to the success of biofilms in diverse habitats; however, many details of the composition, structure, and function remain enigmatic. biofilms formed by the gram-positive bacterium bacillus subtilis depend on the production of the secreted film-forming protein bsla. here, we show that a gradient of electron acceptor availability through the depth of the biofilm gives ri ...201728698374
bacterial cell division: nonmodels poised to take the spotlight.the last three decades have witnessed an explosion of discoveries about the mechanistic details of binary fission in model bacteria such as escherichia coli, bacillus subtilis, and caulobacter crescentus. this was made possible not only by advances in microscopy that helped answer questions about cell biology but also by clever genetic manipulations that directly and easily tested specific hypotheses. more recently, research using understudied organisms, or nonmodel systems, has revealed several ...201728697666
genetic evidence for a novel competence inhibitor in the industrially important bacillus licheniformis.natural genetic competence renders bacteria able to take up and, in case there is sufficient homology to the recipient's chromosome, integrate exogenously supplied dna. well studied in bacillus subtilis, genetic competence is-in several aspects-known to be differently regulated in bacillus licheniformis. we now report on the identification of a novel, chromosomally encoded homolog of a competence inhibitor in b. licheniformis (comi) that has hitherto only been described as a plasmid borne trait ...201728697584
elucidation of bacillus subtilis katmira 1933 potential for spore production in submerged fermentation of plant raw materials.in this study, the effects of several key factors to increase spore production by bacillus subtilis subsp. katmira 1933 were evaluated in shake flask experiments. in a synthetic medium, glucose concentration played a crucial role in the expression of bacilli sporulation capacity. in particular, maximum spore yield (2.3 × 10(9) spores/ml) was achieved at low glucose concentration (2 g/l), and further gradual increase of the carbon source content in the medium caused a decrease in sporulation capa ...201728695539
in vitro imaging of bacteria using (18)f-fluorodeoxyglucose micro positron emission tomography.positron emission tomography (pet) with fluorine-18-fluorodeoxyglucose ((18)f-fdg) can be applied to detect infection and inflammation. however, it was so far not known to what extent bacterial pathogens may contribute to the pet signal. therefore, we investigated whether clinical isolates of frequently encountered bacterial pathogens take up (18)f-fdg in vitro, and whether fdg inhibits bacterial growth as previously shown for 2-deoxy-glucose. 22 isolates of gram-positive and gram-negative bacte ...201728694519
bacillus subtilis iolq (dega) is a transcriptional repressor of iolx encoding nad(+)-dependent scyllo-inositol dehydrogenase.bacillus subtilis is able to utilize at least three inositol stereoisomers as carbon sources, myo-, scyllo-, and d-chiro-inositol (mi, si, and dci, respectively). nad(+)-dependent si dehydrogenase responsible for si catabolism is encoded by iolx. even in the absence of functional iolx, the presence of si or mi in the growth medium was found to induce the transcription of iolx through an unknown mechanism.201728693424
effects of multi-walled carbon nanotubes with various diameters on bacterial cellular membranes: cytotoxicity and adaptive mechanisms.the effect of multi-walled carbon nanotubes (mwnts) with different diameters on the destruction degree toward cellular membranes of bacterial has been explored by investigating the viability of bacteria and the change of composition and surface properties in cellular membranes with the exposure of mwnts. the atrazine degrading bacteria acinetobacter lwoffii dns32 (dns32) is chosen as the model species and escherichia coli (e. coli) and bacillus subtilis (b. subtilis) are selected as the comparis ...201728692883
a highly efficient crispr-cas9-mediated large genomic deletion in bacillus subtilis.in bacillus subtilis, large genomic deletions have been carried out for genome reduction, antibiotic overproduction, and heterologous protein overexpression. in view of the eco-friendliness of b. subtilis, it is critical that engineering preserves its food-grade status and avoids leaving foreign dna in the genome. existing methods of generating large genomic deletions leave antibiotic resistance markers or display low mutation efficiency. in this study, we introduced a clustered regularly inters ...201728690606
biological activity of lipopeptides from bacillus.the lipopeptides of bacillus are small metabolites that contain a cyclic structure formed by 7-10 amino acids (including 2-4 d-amino acids) and a beta-hydroxy fatty acid with 13-19 c atoms. these lipopeptides exhibit a variety of biological activities, including interactions with biofilms, and anti-fungal, anti-inflammatory, anti-tumor, anti-virus, and anti-platelet properties. the multiple activities of lipopeptides have stimulated significant interest in the exploitation of these lipopeptides ...201728685194
perchlorates on mars enhance the bacteriocidal effects of uv light.perchlorates have been identified on the surface of mars. this has prompted speculation of what their influence would be on habitability. we show that when irradiated with a simulated martian uv flux, perchlorates become bacteriocidal. at concentrations associated with martian surface regolith, vegetative cells of bacillus subtilis in martian analogue environments lost viability within minutes. two other components of the martian surface, iron oxides and hydrogen peroxide, act in synergy with ir ...201728684729
noncanonical proteolytic activation of human prothrombin by subtilisin from bacillus subtilis may shift the procoagulant-anticoagulant equilibrium toward thrombosis.blood coagulation is a finely regulated physiological process culminating with the factor xa (fxa)-mediated conversion of the prothrombin (prot) zymogen to active αthrombin (αt). in the prothrombinase complex on the platelet surface, fxa cleaves prot at arg271, generating the inactive precursor prethrombin-2 (pre2), which is further attacked at arg320-ile321 to yield mature αt. whereas the mechanism of physiological prot activation has been elucidated in great detail, little is known about the r ...201728684417
stiffening of bacteria cells as a first manifestation of bactericidal attack.development of new antibacterial agents requires generation of new methods that will allow characterization of mechanisms of novel antibacterial actions as well as observation of pathogen susceptibility to antibiotics. a family of synthetic cationic lipids, such as ceragenins, which mimic the action of membrane-targeting natural cationic antibacterial peptides, shows promise as a new family of bactericidal agents. here we report that treatment of bacillus subtillis cells with human cathelicidin ...201728683405
adaptation of bacillus subtilis to life at extreme potassium limitation.potassium is the most abundant metal ion in every living cell. this ion is essential due to its requirement for the activity of the ribosome and many enzymes but also because of its role in buffering the negative charge of nucleic acids. as the external concentrations of potassium are usually low, efficient uptake and intracellular enrichment of the ion is necessary. the gram-positive bacterium bacillus subtilis possesses three transporters for potassium, ktrab, ktrcd, and the recently discovere ...201728679749
bacillus subtilis protects public goods by extending kin discrimination to closely related species.kin discrimination systems are found in numerous communal contexts like multicellularity and are theorized to prevent exploitation of cooperative behaviors. the kin discrimination system in bacillus subtilis differs from most other such systems because it excludes nonkin cells rather than including kin cells. because nonkin are the target of the system, b. subtilis can potentially distinguish degrees of nonkin relatedness, not just kin versus nonkin. we examined this by testing a large strain co ...201728679746
probiotic therapy reduces inflammation and improves intestinal morphology in rats with induced oral mucositis.the aim of the present study was to evaluate the effect of systemic administration of probiotics (prob) on the progression of experimentally induced oral and intestinal mucositis in rats immunosuppressed by chemotherapy (5-fluorouracil: 5-fu). twenty-four rats were divided into the following groups (n=6): gc (control), gprob, g5fu and g5-fu/prob. groups gprob and g5-fu/prob received 1 g of probiotic incorporated into each 100 g of feed (bacillus subtilis, bifidobacterium bifidum, enterococcus fa ...201728678976
the influence of heavy metals on the bioremediation of polycyclic aromatic hydrocarbons in aquatic system by a bacterial-fungal consortium.co-contamination of polycyclic aromatic hydrocarbons (pahs) with heavy metals (hms) in aquatic environments is a global threat; however, little is understood about pah biodegradation in these sites. in this study, pahs' biodegradation in the presence of hms in water by a metal-tolerant consortium composed of bacillus subtilis and fungus acremonium sp. was investigated. the consortium demonstrated higher tolerance to the tested hms (fe(2+), al(3+), ni(2+), cu(2+), mn(2+) and zn(2+)) than the indi ...201728678633
preparation and performance of biofouling resistant pan/chitosan hollow fiber membranes.the preparation of polyacrylonitrile (pan) hollow fiber (hf) membranes has been carried out by dry-jet wet spinning. pan hf membranes were coated with chitosan biopolymers 2 wt% by dip coating and further crosslinked by chemical reagents (tri sodium polyphosphate). pan hf (virgin) and pan/chitosan coated membrane were characterized by sem and tested for water flux. proteins pepsin, albumin, and clay of 1000 ppm concentration were tested for separation efficiency. in addition, bacterial species e ...201728677086
novel fibrous collagen-based cream accelerates fibroblast growth for wound healing applications: in vitro and in vivo evaluation.the present study reports the development of a novel film-forming bovine collagenous cream (bcc) based on bovine collagen (bc). in this study, collagen was isolated from bovine forestomach tissue, a novel source, and a cream formulation was prepared using some other bioactive ingredients. the electrophoretic pattern of the bc was found to be similar to type i collagen. the purity of bc was examined by amino acid analysis, which confirmed the presence of atelocollagen. the physicochemical propert ...201728676877
the effects of probiotic bacillus subtilis on the cytotoxicity of clostridium perfringens type a in caco-2 cell culture.some bacillus strains have recently been identified for potential use as probiotics and food additives. the present study evaluated the antimicrobial effects of bacillus subtilis atcc 6633 and its metabolite on the enterotoxin and vegetative cells, spore and germinated spore of clostridium perfringens type a in caco-2 cells.201728676033
polyketide derivatives from a marine-sponge-associated fungus pestalotiopsis heterocornis.twelve previously undescribed polyketide derivatives, heterocornols a-l, and seven known analogues were isolated from a culture of the fungus pestalotiopsis heterocornis associated with sponge. their structures were elucidated by a comprehensive spectroscopic data analysis and cd cotton effects. these compounds were evaluated for cytotoxic and antibacterial activities in vitro. among them, heterocornols a-c, f-h, methyl-(2-formyl-3-hydroxyphenyl)propanoate, agropyrenol, and vaccinol g exhibited ...201728675830
morphologies of bacillus subtilis communities responding to environmental variation.bacterial communities exhibit a variety of growth morphologies in constructing robust systems under different environmental conditions. we review the diverse morphologies of bacillus subtilis communities and their mechanisms of self-organization. b. subtilis uses different cell types to suit environmental conditions and cell density. the subpopulation of each cell type exhibits various environment-sensitive properties. furthermore, division of labor among the subpopulations results in flexible d ...201728675458
morphologies and phenotypes in bacillus subtilis biofilms.in this study, we explored bacillus subtilis biofilm growth under various conditions such as the use of substrates with different stiffnesses and nutrient levels using a well-developed optical imaging technique to spatially and temporally track biofilm growth. we also developed a quantitative method to characterize b. subtilis biofilm morphologies under various growth conditions. to determine biofilm rim irregularities, we used the dimensionless p2a ratio, defined as p(2)/4πa, where p is the per ...201728674970
characterization of h-box region mutants of walk inert to the action of waldiomycin in bacillus subtilis.the walk/walr two-component system is essential for cell wall metabolism and thus for cell growth in bacillus subtilis. waldiomycin was previously isolated as an antibiotic that targeted walk, the cognate histidine kinase (hk) of the response regulator, walr, in b. subtilis. to gain further insights into the action of waldiomycin on walk and narrow down its site of action, mutations were introduced in the h-box region, a well-conserved motif of the bacterial hks of walk. the half-maximal inhibit ...201728674376
screening of a bacillus subtilis strain producing multiple types of cyclic lipopeptides and evaluation of their surface-tension-lowering activities.sixty bacillus subtilis strains were investigated for their ability to produce cyclic lipopeptides (clps). among them, b. subtilis nbrc 109107 produced at least three types of clps by high-performance liquid chromatography (hplc) analysis, and these clps were thought to be surfactin, iturin a, and fengycin by polymerase chain reaction amplification of respective clp synthetase-encoding genes. however, after hplc fractionation and purification, structural analysis of the clps revealed that these ...201728674328
septal membrane localization by c-terminal amphipathic α-helices of mind in bacillus subtilis mutant cells lacking minj or diviva.the min system, which inhibits assembly of the cytokinetic protein ftsz, is largely responsible for positioning the division site in rod-shaped bacteria. it has been reported that minj, which bridges diviva and mind, is targeted to the cell poles by an interaction with diviva, and that minj in turn recruits mincd to the cell poles. minc, however, is located primarily at active division sites at the mid-cell when expressed from its native promoter. surprisingly, we found that b. subtilis mind is ...201728674273
bacillus subtilis intramembrane protease rasp activity in escherichia coli and in vitro.rasp is a predicted intramembrane metalloprotease of bacillus subtilis that has been proposed to cleave the stress response anti-sigma factors rsiw and rsiv, the cell division protein ftsl, and remnant signal peptides, within their transmembrane segments. to provide evidence for direct effects of rasp on putative substrates, we developed a heterologous coexpression system. since expression of catalytically-inactive rasp e21a inhibited expression of other membrane proteins in escherichia coli, we ...201728674070
antibacterial activities of pyrenylated coumarins from the roots of prangos hulusii.the dichloromethane extract of the roots of prangos hulusii, a recently described endemic species from turkey, has yielded nine known and one new prenylated coumarins. the structures were elucidated by spectroscopic methods and direct comparison with the reference compounds where available. the root extract and its prenylated coumarins exhibit antimicrobial activity against nine standard and six clinically isolated strains at a concentration between 5 and 125 µg/ml. in particular, the new coumar ...201728671568
optimization of nutritional components of medium by response surface methodology for enhanced production of lactase.lactase has excellent applications in dairy industry and commercially this enzyme is produced from bacterial sources but not in high yields. in this work, the production of lactase was improved by designing of nutrient components in fermentation medium by one factor at a time. lactose and yeast extract were selected as preferable carbon and nitrogen sources for lactase production with tryptophan and mgso4 showing enhanced production. statistical analysis proved to be a useful and powerful tool i ...201728667642
strain and plastic composite support (pcs) selection for vitamin k (menaquinone-7) production in biofilm reactors.menaquinone-7 (mk-7), a subtype of vitamin k, has received a significant attention due to its effect on improving bone and cardiovascular health. current fermentation strategies, which involve static fermentation without aeration or agitation, are associated with low productivity and scale-up issues and hardly justify the commercial production needs of this vitamin. previous studies indicate that static fermentation is associated with pellicle and biofilm formations, which are critical for mk-7 ...201728667350
synthesis and characterization of zro2 nanoparticles-antimicrobial activity and their prospective role in dental care.nanomaterials are exerting a pull on deal with biological and pharmaceutical applications. biomedical grade of zirconia reveals potential mechanical features of oxide ceramics. in this study, antimicrobial activity and anti-tooth decay applications of the synthesized nps of zro2 were determined. the as-prepared zro2 nps were characterized by uv-vis spectroscopy, ftir and xrd, which determined the formation of zro2nps and their crystalline nature. sem analysis further revealed spherical shaped np ...201728666841
poly-γ-glutamic acid productivity of bacillus subtilis bse1 has positive function in motility and biocontrol against fusarium graminearum.in this study, we investigate the relationship between γ-pga productivity and biocontrol capacity of bacillus subtilis bse1; one bacterial isolate displayed 62.14% biocontrol efficacy against fusarium root rot. the γ-pga yield assay, motility assay, wheat root colonization assay, and biological control assay were analysed in different γ-pga yield mutants of bse1. the pgsb (pga-synthase-capb gene) deleted mutant of bse1 reduced γ-pga yield and exhibited apparent decline of in vitro motile ability ...201728664519
modeling and optimization of fermentation variables for enhanced production of lactase by isolated bacillus subtilis strain vuvd001 using artificial neural networking and response surface methodology.modeling and optimization were performed to enhance production of lactase through submerged fermentation by bacillus subtilis vuvd001 using artificial neural networks (ann) and response surface methodology (rsm). the effect of process parameters namely temperature (°c), ph, and incubation time (h) and their combinational interactions on production was studied in shake flask culture by box-behnken design. the model was validated by conducting an experiment at optimized process variables which gav ...201728664372
multifarious allelochemicals exhibiting antifungal activity from bacillus subtilis mbcu5.a potential antagonist, designated strain bacillus subtilis mbcu5 was previously isolated from vermicompost-amended soils of gandhinagar, gujarat, india. crude allelochemicals from strain mbcu5 displayed strong antifungal activity against macrophomina phaseolina as well as rhizoctonia solani. these crude allelochemicals were tentatively identified as iturin, fengycin and surfactin through tlc and hptlc analysis. lipopeptides produced by mbcu5 were identified by maldi-tof-ms and lc-esi-ms/ms anal ...201728664362
overproduction of rummeliibacillus pycnus arginase with multi-copy insertion of the arg (r.pyc) cassette into the bacillus subtilis chromosome.a plasmid-less and marker-less strain with multi-copy integration of the arginase gene from rummeliibacillus pycnus was constructed using bacillus subtilis 168 as a host. a total of nine copies of the arg (r.pyc) cassettes, in which the r. pycnus arginase gene was fused with the strong promoter p43, were inserted into the recipient chromosome. these multiple insertions were completed via step-by-step integrations into designed (2 copies) and random (9 copies) sites, respectively. a strategy for ...201728664323
three new abietane-type diterpenoids from plectranthus africanus and their antibacterial activities.three new abietane-type diterpenoids, plectranthroyleanones a - c (1 - 3), together with five known compounds (4 - 8) were isolated from the methanol extract of the whole plant of plectranthus africanus using column chromatography techniques. the structures of the new compounds were elucidated using a combination of 1d and 2d nmr and hresims analyses. compound 1 exhibited weak activities with minimal inhibitory concentration values of 75 µg/ml against gram-positive bacteria, bacillus subtilis an ...201728662528
correction: saccharomyces boulardii and bacillus subtilis b10 modulate tlrs and cytokines expression patterns in jejunum and ileum of broilers.[this corrects the article doi: 10.1371/journal.pone.0173917.].201728662205
innovative non-thermal plasma disinfection process inside sealed bags: assessment of bactericidal and sporicidal effectiveness in regard to current sterilization norms.in this work, we developed a device capable to generate a non-thermal plasma discharge inside a sealed bag. the aim of this study was to assess the effectiveness of the oxygen, nitrogen and argon plasma sterilization on pseudomonas aeruginosa, staphylococcus aureus and bacillus subtilis spores according to the nf en 556 norm. moreover the bag integrity which is a critical key to maintain the sterile state of items after the end of the process was verified by fourier transform infrared (ftir) and ...201728662202
fluoro-carba-sugars are glycomimetic activators of the glms ribozyme.the glms ribozyme is a bacterial gene-regulating riboswitch that controls cell wall synthesis, depending on glucosamine-6-phosphate as a cofactor. due to the presence of this ribozyme in several human pathogen bacteria (e.g. mrsa, vrsa), the glms ribozyme represents an attractive target for the development of artificial cofactors. the substitution of the ring oxygen in carbohydrates by functionalized methylene groups leads to a new generation of glycomimetics that exploits distinct interaction p ...201728661578
controllable synthesis fe3o4@pohaba core-shell nanostructure as high-performance recyclable bifunctional magnetic antimicrobial agent.we demonstrated a method to form magnetic antimicrobial pohaba (poly-n,n'-[(4,5-dihydroxy-1,2-phenylene)bis(methylene)]bisacrylamide)-based core-shell nanostructure by free-radical polymerization of ohaba on the fe3o4 core surface. the magnetic antimicrobial agent fe3o4@pohaba can be used in domestic water treatment against bacterial pathogens. the thickness of pohaba shell could be controlled from 10.4 ± 1.2 to 56.3 ± 11.7 nm by the dosage of ohaba. the results of antimicrobial-activity test in ...201728660503
establishment of a markerless gene deletion system in chromohalobacter salexigens dsm 3043.chromohalobacter salexigens dsm 3043 can grow over a wide range of salinity, which makes it as an excellent model organism for understanding the mechanism of prokaryotic osmoregulation. functional analysis of c. salexigens genes is an essential way to reveal their roles in cellular osmoregulation. however, the lack of an effective markerless gene deletion system has prevented construction of multiple gene deletion mutants for the members in the genus. here, we report the development of a markerl ...201728660361
a thermophilic cell-free cascade enzymatic reaction for acetoin synthesis from pyruvate.acetoin (3-hydroxy-2-butanone) is an important bio-based platform chemical with wide applications. in vitro enzyme catalysed synthesis exhibits great feasibility in the production of chemicals with high purity. in the present work, a synthetic pathway involving a two-step continuous reaction was constructed in vitro for acetoin production from pyruvate at improved temperature. thermostable candidates, acetolactate synthase (coahasl1 and coahasl2 from caldicellulosiruptor owensensis ol) and α-ace ...201728659601
biopolymer zein-coated gold nanoparticles: synthesis, antibacterial potential, toxicity and histopathological effects against the zika virus vector aedes aegypti.the control of multidrug-resistant bacteria as well as insect pests and vectors is timely and important now a days. the present study was designed to evaluate the in vitro antibacterial, antibiofilm and mosquito larvicidal effects of gold nanoparticles synthesized using the zein biopolymer (ze-aunps) against gram positive (bacillus pumilus and bacillus subtilis), gram negative (shigella sonnei and pseudomonas aeruginosa) bacteria and third instar larvae of the dengue and zika virus vector aedes ...201728654862
identification and growth optimization of a marine bacillus dk1-sa11 having potential of producing broad spectrum antimicrobial compounds.control of harmful bacteria in food, aquaculture, pharmaceuticals, agriculture, hospitals and recreation water pools are of great global concern. marine bacteria are an enormous source of bio-controlling agents. the aim of this study was to identify and optimize the growth conditions including effect of different biotic and abiotic factors on antimicrobial activity of strain dk1-sa11 isolated from qingdao bay of china yellow sea. microscopic characterization, api® 20e and 50 chb kit base carbohy ...201728653930
screening of leaves extracts from calamus aromaticus for their antimicrobial activity by disc diffusion assay.the current research investigates the anti-microbial activities of different solvent extracted samples from the leaves of calamus aromaticus against gram positive, gram negative bacteria and fungi using 500, 1000 and 2000 μg disc(-1) concentrations. escherichia coli, citorbacter freundii and candida albicans showed resistivity to crude methanolic extract and the same microbes were more susceptible to water extracted fractions. maximum activity was measured by hexane extracted fractions against p ...201728653924
kitchen phytochemicals from allium cepa - their role in multidrug resistance.bacterial resistance to antibiotics is increasingly becoming a concern to public health. current antibiotics have failed to bring desirable results to many bacterial infections due to multi-resistant strains. so, concerted efforts are being made to curve this with the help of dietary phytohemicals. a number of dietary phytochemical are being put to trials for antimicrobial activity; however it is worthwhile to search such a part of diet that is very frequently used by major population. keeping t ...201728653923
evaluation of anti-oxidative, antimicrobial and anti-diabetic potential of adiantum venustum and identification of its phytochemicals through gc-ms.the present study aimed to explore antioxidant, antimicrobial and anti-diabetic properties of the fern adiantum venustum. the tpc (total phenolic content) of methanolic extract of the plant was 247.95±0.0007μg of gallic acid equivalents per gram of dried extract (mg gae/g). the highest tpc was in n-butanolic fraction, which was 981.45±0.1562mg gae/g. hexane fraction showed lowest tpc (256.95±0.0420mg gae/g). ethyl acetate fraction exhibited highest total flavonoid content (tfc), i.e., 62.0±0.050 ...201728653913
x-ray crystal structure of a reiterative transcription complex reveals an atypical rna extension pathway.reiterative transcription is a noncanonical form of rna synthesis in which a nucleotide specified by a single base in the dna template is repetitively added to the nascent transcript. here we determined the crystal structure of an rna polymerase, the bacterial enzyme from thermus thermophilus, engaged in reiterative transcription during transcription initiation at a promoter resembling the pyrg promoter of bacillus subtilis the structure reveals that the reiterative transcript detours from the d ...201728652344
a novel expression vector for the secretion of abaecin in bacillus subtilis.this study aimed to describe a bacillus subtilis expression system based on genetically modified b. subtilis. abaecin, an antimicrobial peptide obtained from apis mellifera, can enhance the effect of pore-forming peptides from other species on the inhibition of bacterial growth. for the exogenous expression, the abaecin gene was fused with a tobacco etch virus protease cleavage site, a promoter pglv, and a mature beta-glucanase signal peptide. also, a b. subtilis expression system was constructe ...201728651889
the toxicity of cationic surfactant hdtma-br, desorbed from surfactant modified zeolite, towards faecal indicator and environmental microorganisms.surfactant modified zeolite (smz) represents a versatile, cost-effective permeable reactive material, capable of treating multiple classes of contaminants. the potential for hdtma-br, a cationic surfactant commonly used to modify zeolite, to desorb from the zeolite surface has been identified as a potential issue for the ongoing use of smz in water remediation contexts. this paper investigates the toxicity of hdtma-br towards enteric virus surrogates, f-rna bacteriophage ms2 and e. coli, bacillu ...201728651198
elucidating sequence and solvent specific design targets to protect and stabilize enzymes for biocatalysis in ionic liquids.for many different frameworks, the structure, function, and dynamics of an enzyme is largely determined by the nature of its interactions with the surrounding host environment, thus a molecular level understanding of enzyme/host interactions is essential to the design of new processes and applications. ionic liquid (il) solvents are a popular class of solvents in which to study enzyme behavior, yet it is still not possible to predict how a given enzyme will behave in a given il solvent. furtherm ...201728650512
surface topology affects wetting behavior of bacillus subtilis biofilms.the colonization of surfaces by bacterial biofilms constitutes a huge problem in healthcare and industry. when attempting biofilm inactivation or removal, it is crucial to sufficiently wet the biofilm surface with antibacterial agents; however, certain biofilms efficiently resist wetting, and the origin of this behavior remains to date unclear. here, we demonstrate that, depending on the growth medium used, the model bacterium bacillus subtilis can form biofilm colonies with distinct surface pro ...201728649412
bacillus subtilis utilizes the dna damage response to manage multicellular development.bacteria switch between free-living and a multicellular state, known as biofilms, in response to cellular and environmental cues. it is important to understand how these cues influence biofilm development as biofilms are not only ubiquitous in nature but are also causative agents of infectious diseases. it is often believed that any stress triggers biofilm formation as a means of bacterial protection. in this study, we propose a new mechanism for how cellular and environmental dna damage may inf ...201728649409
bacillus subtilis biofilm development in the presence of soil clay minerals and iron oxides.clay minerals and metal oxides, as important parts of the soil matrix, play crucial roles in the development of microbial communities. however, the mechanism underlying such a process, particularly on the formation of soil biofilm, remains poorly understood. here, we investigated the effects of montmorillonite, kaolinite, and goethite on the biofilm formation of the representative soil bacteria bacillus subtilis. the bacterial biofilm formation in goethite was found to be impaired in the initial ...201728649405
kinetic modelling and meta-analysis of the b. subtilis siga regulatory network during spore germination and outgrowth.this study describes the meta-analysis and kinetic modelling of gene expression control by sigma factor siga of bacillus subtilis during germination and outgrowth based on microarray data from 14 time points. the analysis computationally models the direct interaction among siga, siga-controlled sigma factor genes (sigm, sigh, sigd, sigx), and their target genes. of the >800 known genes in the siga regulon, as extracted from databases, 311 genes were analysed, and 190 were confirmed by the kineti ...201728648455
yvck, a protein required for cell wall integrity and optimal carbon source utilization, binds uridine diphosphate-sugars.in bacillus subtilis, listeria monocytogenes and in two mycobacteria, it was previously shown that yvck is a gene required for normal cell shape, for optimal carbon source utilization and for virulence of pathogenic bacteria. here we report that the b. subtilis protein yvck binds to uridine diphosphate-sugars like uridine diphosphate-glucose (udp-glc) and uridine diphosphate-n-acetylglucosamine (udp-glcnac) in vitro. using the crystal structure of bacillus halodurans yvck, we identified residues ...201728646159
post-translational modification of ribosomally synthesized peptides by a radical sam epimerase in bacillus subtilis.ribosomally synthesized peptides are built out of l-amino acids, whereas d-amino acids are generally the hallmark of non-ribosomal synthetic processes. here we show that the model bacterium bacillus subtilis is able to produce a novel type of ribosomally synthesized and post-translationally modified peptide that contains d-amino acids, and which we propose to call epipeptides. we demonstrate that a two [4fe-4s]-cluster radical s-adenosyl-l-methionine (sam) enzyme converts l-amino acids into thei ...201728644475
abilities of co-cultures of brown-rot fungus fomitopsis pinicola and bacillus subtilis on biodegradation of ddt.ddt (1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane) is one of the pesticides that are hazardous for the environment and human health. effective environmental-friendly treatment using co-cultures of fungi and bacteria is needed. in this study, the bacteria bacillus subtilis at various volumes of 1, 3, 5, 7, and 10 ml (1 ml ≈ 6.7 × 10(8) cfu) were mixed into 10 ml of the brown-rot fungus fomitopsis pinicola culture for degrading ddt during a 7-days incubation period. ddt was degraded by approximat ...201728642970
editorial: spores and spore formers. 201728642754
1,1ꞌ-carbonyldiimidazole (cdi) mediated facile synthesis, structural characterization, antimicrobial activity and in-silico studies of coumarin-3-carboxamide derivatives.despite of the availability of variety of antibacterial agents, re-emergance of pathogenic bacteria is still a serious medical concern. so, identification of new, safer, and selective antibacterial agents is the key interest in the medicinal chemistry research.201728641525
novel antimicrobial activities of a peptide derived from a japanese soybean fermented food, natto, against streptococcus pneumoniae and bacillus subtilis group strains.we recently isolated a tumoricidal peptide from natto, a japanese traditional fermented food. in the present study, antimicrobial activity of the natto peptide was examined. the peptide consisted of 45 amino acid residues, and its structure was predicted to be rich in α-helix. it excreted antimicrobial activity only against streptococcus pneumoniae and bacillus subtilis group (b. subtilis, bacillus pumilus, and bacillus licheniformis). lesser antimicrobial activity was observed for streptococcus ...201728641406
chemical composition and biological activities of leaf and fruit essential oils from eucalyptus camaldulensis.the chemical composition of the essential oils from the leaves and fruit of eucalyptus camaldulensis grown in mersin, turkey was analyzed using gas chromatography (gc) and gas chromatography-mass spectrometry (gc-ms) techniques. the biological activities (antibacterial and antifungal) were examined using the agar well diffusion method. the main leaf oil constituents were p-cymene (42.1%), eucalyptol (1,8-cineole) (14.1%), α-pinene (12.7%) and α-terpinol (10.7%). the main constituents of the frui ...201728640755
purification and characterization of two new cell-bound bioactive compounds produced by wildlactococcus lactisstrain.novel compounds and innovative methods are required considering that antibiotic resistance has reached a crisis point. in the study, two cell-bound antimicrobial compounds produced by l. lactis id1.5 were isolated and partially characterized. following purification by cationic exchange and a solid phase c18 column, antimicrobial activity was recovered after three runs of rpc using 60% (v/v) and 100% (v/v) of 2-propanol for elution, suggesting that more than one antimicrobial compound were produc ...201728637209
green catanionic gemini surfactant-lichenysin mixture: improved surface, antimicrobial, and physiological properties.catanionic surfactant mixtures form a wide variety of organized assemblies and aggregates with improved physicochemical and biological properties. the green catanionic mixture n(α)n(ω)-bis(n(α)caproylarginine) α,ω-propyldiamide (c3(ca)2):lichenysin (molar ratio 8:2) showed antimicrobial synergies against yersinia enterocolitica, bacillus subtilis, escherichia coli o157:h7, and candida albicans. flow cytometry and viability studies indicated that this catanionic mixture increases the probability ...201728636319
biocontrol of the wheat pathogen zymoseptoria tritici using cyclic lipopeptides from bacillus subtilis.innovation toward ecofriendly plant protection products compatible with sustainable agriculture and healthy food is today strongly encouraged. here, we assessed the biocontrol activity of three cyclic lipopeptides from bacillus subtilis (mycosubtilin, m; surfactin, s; fengycin, f) and two mixtures (m + s and m + s + f) on wheat against zymoseptoria tritici, the main pathogen on this crop. foliar application of these biomolecules at a 100-mg l(-1) concentration on the wheat cultivars dinosor and ...201728634804
coumarin-benzimidazole hybrids as a potent antimicrobial agent: synthesis and biological elevation.molecular hybridization approach is an emerging tool in drug discovery for designing new pharmacophores with biological activity. a novel, new series of coumarin-benzimidazole hybrids were designed, synthesized and evaluated for their broad spectrum antimicrobial activity. among all the synthesized molecules, compound (e)-3-(2-1h-benzo[d]imidazol-1-yl)-1-((4-chlorobenzyl)oxy)imino)ethyl)-2h-chromen-2-one showed the most promising broad spectrum antibacterial activity against pseudomonas aerugino ...201728634338
experimental evolution of bacillus subtilis.the endospore-forming bacteria have persisted on earth perhaps 3ga, leveraging the flexibility of their distinctive lifestyle to adapt to a remarkably wide range of environments. this process of adaptation can be investigated through the simple but powerful technique of laboratory evolution. evolved strains can be analyzed by whole genome sequencing and an array of omics technologies. the intensively studied, genetically tractable endospore-former, bacillus subtilis, is an ideal subject for labo ...201728631363
variation analysis of bacterial polyhydroxyalkanoates production using saturated and unsaturated hydrocarbons.polyhydroxyalkanoates (pha) are efficient, renewable and environment friendly polymeric esters. these polymers are synthesized by a variety of microbes under stress conditions. this study was carried out to check the suitability of waste frying oil in comparison to other oils for economical bioplastic production. six bacterial strains were isolated and identified as bacillus cereus (kf270349), klebsiella pneumoniae (kf270350), bacillus subtilis (kf270351), brevibacterium halotolerance (kf270352) ...201728629970
an enhanced vector-free allele exchange (vfae) mutagenesis protocol for genome editing in a wide range of bacterial species.vector-free allele exchange (vfae) is a newly developed protocol for genome editing in pseudomonas species. although several parameters have been determined to optimize the procedures for obtaining a stable and high-frequency mutation, numerous false-positive clones still appear on the plate, which increases the difficulty of finding the desired mutants. it has also not been established whether this protocol can be used for genome editing in other bacterial species. in the current study, the pro ...201728629206
lc-ms/ms proteomic analysis of starved bacillus subtilis cells overexpressing ribonucleotide reductase (nrdef): implications in stress-associated mutagenesis.the non-appropriate conditions faced by nutritionally stressed bacteria propitiate error-prone repair events underlying stationary-phase- or stress-associated mutagenesis (spm). the genetic and molecular mechanisms involved in spm have been deeply studied but the biochemical aspects of this process have so far been less explored. previous evidence showed that under conditions of nutritional stress, non-dividing cells of strain b. subtilis yb955 overexpressing ribonucleotide reductase (rnr) exhib ...201728624879
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