Publications

TitleAbstractYear(sorted descending)
Filter
PMID
Filter
low-cost and eco-friendly green synthesis of silver nanoparticles using prunus japonica (rosaceae) leaf extract and their antibacterial, antioxidant properties.low cost and eco-friendly green synthesis of silver nanoparticles (agnps) from silver nitrate (agno3) using prunus japonica leaves extract as reducing agent by a simple method at room temperature. the biosynthesized nanoparticles (nps) were characterized by uv-vis, tunneling electron microscopy (hr-tem), scanning electron microscopy (sem) coupled with x-ray energy dispersive spectrophotometer (edax), x-ray diffraction (xrd) and fourier transform infrared spectroscopy (ftir). in uv-vis spectrosco ...201627396523
common mechanism of cross-resistance development in pathogenic bacteria bacillus cereus against alamethicin and pediocin involves alteration in lipid composition.to understand the mechanism of development of cross-resistance in food pathogen bacillus cereus against an antimicrobial peptide pediocin and antibiotic alamethicin, the present study was designed. pediococcus pentosaceus was taken as a source of pediocin, and it was purified by ammonium sulphate precipitation followed by cation exchange chromatography with 14.01-fold purity and 14.4 % recovery. b. cereus strains alamethicin-resistant strains (ic50 3.23 µg/ml) were selected from sensitive popula ...201627378130
comparative genomics of iron-transporting systems in bacillus cereus strains and impact of iron sources on growth and biofilm formation.iron is an important element for bacterial viability, however it is not readily available in most environments. we studied the ability of 20 undomesticated food isolates of bacillus cereus and two reference strains for capacity to use different (complex) iron sources for growth and biofilm formation. studies were performed in media containing the iron scavenger 2,2-bipyridine. transcriptome analysis using b. cereus atcc 10987 indeed showed upregulation of predicted iron transporters in the prese ...201627375568
combined effect of anaerobiosis, low ph and cold temperatures on the growth capacities of psychrotrophic bacillus cereus.psychrotrophic strains of the foodborne pathogen bacillus cereus can multiply during the refrigerated storage of food products. the aim of this study was to determine the impact of anaerobiosis on the growth of two psychrotrophic b. cereus strains exposed to acidic ph at a cold temperature in a laboratory medium. at 10 °c, growth occurred at ph values equal to or higher than 5.7 during anaerobiosis, whereas aerobic growth was observed from ph 5.4. growth rates during aerobiosis were similar at p ...201627375252
effect of endophytic bacillus cereus erbp inoculation into non-native host: potentials and challenges for airborne formaldehyde removal.phytoremediation could be a cost-effective, environmentally friendly approach for the treatment of indoor air. however, some drawbacks still dispute the expediency of phytotechnology. our objectives were to investigate the competency of plant growth-promoting (pgp) endophytic bacillus cereus erbp (endophyte root blue pea), isolated from the root of clitoria ternatea, to colonize and stabilize within zamioculcas zamiifolia and euphorbia milii as non-native hosts without causing any disease or str ...201627362296
genetic and functional analyses of krs, a locus encoding kurstakin, a lipopeptide produced by bacillus thuringiensis.bacteria of the bacillus genus are able to synthesize several families of lipopeptides. these small molecules are the product of non-ribosomal peptide synthetases. in 2000, it was found that bacillus thuringiensis, an entomopathogenic bacterium of the bacillus cereus group, produced a previously unknown lipopeptide: kurstakin. genomic analyses reveal that the krs locus, encoding the kurstakin synthetases, is specific to the b. cereus group, but is unevenly distributed within this group. previous ...201627353188
modulation of phytoremediation and plant growth by the treatment with pgpr, ag nanoparticle and untreated municipal wastewater.the present attempt was made to determine the effects of untreated municipal wastewater (mw) on growth and physiology of maize and to evaluate the role of ag nanoparticle and plant-growth-promoting rhizobacteria (pgpr) when interacting with mw used for irrigation. it was used for the isolation of pgpr. the isolates were identified and characterized based on the colony morphology, c/n source utilization pattern using miniaturized identification system (qts 24), catalase (cat) and oxidase tests, a ...201627348506
photobiologic-mediated fabrication of silver nanoparticles with antibacterial activity.we present the simple, eco-friendly synthesis of silver nanoparticles (agnps) using sunlight or green, red, blue, or white led light together with dryopteris crassirhizoma rhizome extract (dcre) as the reducing and capping agent. the preliminary indication of agnp production was a color change from yellowish green to brown after light exposure in the presence of dcre. optimization of parameters such as ph, inoculum dose, and metal ion concentration played an important role in achieving nanoparti ...201627348063
chemical profile, antioxidant and antibacterial activities of achillea moschata wulfen, an endemic species from the alps.aerial parts of achillea moschata wulfen (asteraceae) growing wild in the italian rhaetian alps were investigated to describe, for the first time, their phenolic content, as well as to characterize the essential oil. inspection of the metabolic profile combining hplc-dad and esi-ms/ms data showed that the methanol extract contained glycosylated flavonoids with luteolin and apigenin as the main aglycones. among them, the major compound was 7-o-glucosyl apigenin. caffeoyl derivates were other phen ...201627347915
enhanced efficacy of clindamycin hydrochloride encapsulated in pla/plga based nanoparticle system for oral delivery.clindamycin hydrochloride (clh) is a clinically important oral antibiotic with wide spectrum of antimicrobial activity that includes gram-positive aerobes (staphylococci, streptococci etc.), most anaerobic bacteria, chlamydia and certain protozoa. the current study was focused to develop a stabilised clindamycin encapsulated poly lactic acid (pla)/poly (d,l-lactide-co-glycolide) (plga) nano-formulation with better drug bioavailability at molecular level. various nanoparticle (nps) formulations o ...201627463797
prodigiosin inhibits motility and activates bacterial cell death revealing molecular biomarkers of programmed cell death.the antimicrobial activity of prodigiosin from serratia nematodiphila darsh1, a bacterial pigment was tested against few food borne bacterial pathogens bacillus cereus, staphylococcus aureus, pseudomonas aeruginosa and escherichia coli. the mode of action of prodigiosin was studied. prodigiosin induced bactericidal activity indicating a stereotypical set of biochemical and morphological feature of programmed cell death (pcd). pcd involves dna fragmentation, generation of ros, and expression of a ...201627460563
lactobacillus delbrueckii subsp lactis cidca 133 modulates response of human epithelial and dendritic cells infected with bacillus cereus.it is known that probiotic microorganisms are able to modulate pathogen virulence. this ability is strain dependent and involves multiple interactions between microorganisms and relevant host's cell populations. in the present work we focus on the effect of a potentially probiotic lactobacillus strain (lactobacillus delbrueckii subsp. lactis cidca 133) in an in vitro model of bacillus cereus infection. our results showed that infection of intestinal epithelial ht-29 cells by b. cereus induces nu ...201627459335
chemical composition and biological activity of centaurea baseri: new species from turkey.the genus centaurea l. is one of the largest and important genera of asteraceae family. centaurea species have been widely used as herbal remedies in folk medicine for their antidandruff, antidiarrheic, antirheumatic, anti-inflammatory, choleretic, diuretic, digestive, stomachic, astringent, antipyretic, cytotoxic, and antibacterial properties. centaurea baseri kose & alan is a recently described local endemic species in turkey and this is the first study on the chemical composition and bioactiv ...201627447348
the comer gene plays an important role in biofilm formation and sporulation in both bacillus subtilis and bacillus cereus.bacteria adopt alternative cell fates during development. in bacillus subtilis, the transition from planktonic growth to biofilm formation and sporulation is controlled by a complex regulatory circuit, in which the most important event is activation of spo0a, a transcription factor and a master regulator for genes involved in both biofilm formation and sporulation. in b. cereus, the regulatory pathway controlling biofilm formation and cell differentiation is much less clear. in this study, we sh ...201627446060
expression of the genes encoding the cask/r two-component system and the desa desaturase during bacillus cereus cold adaptation.two-component systems (tcs) allow a cell to elaborate a variety of adaptive responses to environment changes. the recently discovered cask/r tcs plays a role in the optimal unsaturation of fatty acids necessary for cold adaptation of the foodborne-pathogen bacillus cereus here, we showed that the promoter activity of the operon encoding this tcs was repressed during growth at low temperature in the stationary phase in the parental strain when compared to the cask/r mutant, suggesting that casr n ...201627435329
evolutionary processes and environmental factors underlying the genetic diversity and lifestyles of bacillus cereus group bacteria.bacteria of the bacillus cereus group are environmental gram-positive spore-forming bacteria ubiquitously distributed. despite the high degree of genetic similarity among the different strains, they show strong phenotypic variability, from mammal or entomopathogen strains to soil-dwelling saprophytes, and from psychrophylic to thermotolerant strains. most of the phenotypes are linked to the presence of large plasmids that encode for diverse toxins. however, other processes, like mutation or reco ...201627424810
bacillus spore wet heat resistance and evidence for the role of an expanded osmoregulatory spore cortex.previous work reported that decoated bacillus cereus spores incubated in 4 mol l(-1) cacl2 are killed at lower temperatures than spores in water. this wet heat sensitization was suggested to support a role for an osmoregulatory peptidoglycan cortex in spore cores' low water content, and their wet heat resistance. current work has replicated this finding with spores of b. cereus, bacillus megaterium and bacillus subtilis. however, this work found that decoated spores apparently killed at 80°c in ...201627424522
complete genome sequences of nine bacillus cereus group phages.we report the sequences of nine novel bacillus cereus group bacteriophages: dignkc, juglone, nemo, nigalana, notthecreek, phrodo, sagefayge, vinny, and zuko. these bacteriophages are double-stranded dna-containing myoviridae isolated from soil samples using b. thuringiensis subsp. kurstaki as the host bacterium.201627417827
deciphering the interactions between the bacillus cereus linear plasmid, pbclin15, and its host by high-throughput comparative proteomics.the pathogen, bacillus cereus, is able to adapt its metabolism to various environmental conditions. the reference strain, bacillus cereus atcc 14579, harbors a linear plasmid, pbclin15, which displays a cryptic prophage behavior. here, we studied the impact of pbclin15 on the aerobic respiratory metabolism of b. cereus by curing its host strain. we compared, by means of a high-throughput shotgun proteomic approach, both the cellular proteome and the exoproteome of b. cereus atcc 14579 in the pre ...201627321915
activity of flavanones isolated from rhododendron hainanense against plant pathogenic fungi.in a search for naturally occurring antimicrobial compounds in medicinal plants and herbs, seven flavanones were isolated from the aerial parts of rhododendron hainanense and were tested for their antimicrobial activities against six bacteria and six plant pathogenic fungi. within the series of flavanones tested, farrerol (1) displayed moderate antibacterial activities against bacillus cereus, b. subtilis, pseudomonas aeruginosa, staphylococcus aureus, escherichia coli and erwinia carotovora, wi ...201627319130
anti-bacterial and anti-biofilm activity of probiotic bacteria against oral pathogens.in this study, three lactic acid bacteria (lab), isolated from barley, traditional dried meat and fermented olive were characterized and tested for their anti-bacterial and anti-biofilm activities against oral bacteria. our results revealed that the tested lab were γ-hemolytic and were susceptible to four antibiotics. all the strains were resistant to low ph, bile salt, pepsin and pancreatin. furthermore, fb2 displayed a high aut-oaggregative phenotype (99.54%) while ff2 exhibited the best co-ag ...201627317856
novel group of leaderless multipeptide bacteriocins from gram-positive bacteria.from raw milk we found 10 lactococcus garvieae isolates that produce a new broad-spectrum bacteriocin. though the isolates were obtained from different farms, they turned out to possess identical inhibitory spectra, fermentation profiles of sugars, and repetitive sequence-based pcr (rep-pcr) dna patterns, indicating that they produce the same bacteriocin. one of the isolates (l. garvieae ks1546) was chosen for further assessment. purification and peptide sequencing combined with genome sequencin ...201627316965
biological activities of aerial parts extracts of euphorbia characias.the aim of the present study was to evaluate antioxidant, antimicrobial, anti-hiv, and cholinesterase inhibitory activities of aqueous and alcoholic extracts from leaves, stems, and flowers of euphorbia characias. the extracts showed a high antioxidant activity and were a good source of total polyphenols and flavonoids. ethanolic extracts from leaves and flowers displayed the highest inhibitory activity against acetylcholinesterase and butyrylcholinesterase, showing potential properties against ...201627314007
complete genome sequence of bacteriophage deep-blue infecting emetic bacillus cereus.the bacillus cereus emetic pathotype is responsible for important food-borne intoxications. here, we describe the complete genome sequence of bacteriophage deep-blue, which is able to infect emetic strains of b. cereus deep-blue is a 159-kb myophage of the bastille-like group within the spounavirinae.201627313285
interactions between bacillus cereus cgmcc 1.895 and clostridium beijerinckii ncimb 8052 in coculture for butanol production under nonanaerobic conditions.low oxygen tolerance and substrate restriction continues to hamper the process of biobutanol industrialization. in this work, butanol fermentation with cocultures of bacillus cereus china general microbiological culture collection center (cgmcc) 1.895 and clostridium beijerinckii ncimb 8052 under nonanaerobic conditions was investigated, and the interactions between these two strains were examined. the addition of b. cereus cgmcc 1.895 resulted in higher oxygen tolerance and a wider range of sub ...201627306691
a rapid multiplex real-time pcr high-resolution melt curve assay for the simultaneous detection of bacillus cereus, listeria monocytogenes, and staphylococcus aureus in food.three important foodborne pathogens, bacillus cereus, listeria monocytogenes, and staphylococcus aureus, are of great concern for food safety. they may also coexist in food matrices and, in the case of b. cereus and s. aureus, the resulting illnesses can resemble each other owing to similar symptoms. therefore, their simultaneous detection may have advantages in terms of cost savings and rapidity. given this context, a rapid multiplex real-time pcr high-resolution melt curve assay for the simult ...201627296430
assessment of the microbiological quality of meat pies from retail sale in england 2013.outbreaks of foodborne illness caused by bacillus cereus and listeria monocytogenes in england associated with meat pie consumption were detected in 2012. to obtain baseline data for pies unrelated to outbreaks, 862 samples of ready-to-eat meat pies were collected at retail or from catering facilities in england in 2013 and examined to enumerate food-poisoning bacteria and indicator organisms using organization for standardization (iso) methods for listeria spp. including l. monocytogenes (iso 1 ...201627296425
evolution of the spoiisabc toxin-antitoxin-antitoxin system in bacilli.programmed cell death in bacteria is generally associated with two-component toxin-antitoxin systems. the spoiisabc system, originally identified in bacillus subtilis, consists of three components: a spoiisa toxin and the spoiisb and spoiisc antitoxins. spoiisa is a membrane-bound protein, while spoiisb and spoiisc are small cytosolic antitoxins, which are able to bind spoiisa and neutralize its toxicity. in the presented bioinformatics analysis, a taxonomic distribution of the genes of the spoi ...201627294956
innovative bionanocomposite films of edible proteins containing liposome-encapsulated nisin and halloysite nanoclay.films and coatings based on natural polymers have gained increased interest for food packaging applications. in this work, halloysite and phosphatidylcholine liposomes encapsulating nisin were used to develop nanocomposite films of gelatin and casein. liposomes prepared with either soybean lecithin or phospholipon(®) showed particle size ranging from 124 to 178nm and high entrapment efficiency (94-100%). considering their stability, phospholipon(®) liposomes with 1.0mg/ml nisin were selected for ...201627289315
mob/orit, a mobilizable site-specific recombination system for unmarked genetic manipulation in bacillus thuringiensis and bacillus cereus.bacillus thuringiensis and bacillus cereus are two important species in b. cereus group. the intensive study of these strains at the molecular level and construction of genetically modified bacteria requires the development of efficient genetic tools. to insert genes into or delete genes from bacterial chromosomes, marker-less manipulation methods were employed.201627286821
whole-genome sequences of seven strains of bacillus cereus isolated from foodstuff or poisoning incidents.we present here the whole shotgun genome sequences of seven strains of bacillus cereus isolated from foodstuff samples or food poisoning incidents.201627284145
time to stop holding the elevator: a new piece of the transport protein mechanism puzzle.in this issue of structure, mccoy et al. (2016) describe the 2.55-å x-ray structure of the outward-facing occluded conformation of the bacillus cereus maltose transporter malt. this structure represents the penultimate piece needed to complete the picture of the transport cycle of the glucose superfamily of membrane-spanning eiic components.201627276425
evaluation of the toxicity and toxicokinetics of cereulide from an emetic bacillus cereus strain of milk origin.bacillus cereus is an opportunistic foodborne agent causing food poisoning and many infectious diseases. the heat-stable emetic toxin cereulide is one of the most prevalent toxins produced by pathogenic b. cereus, resulting in symptoms such as emesis and liver failure. in the present work, the toxicity and toxicokinetics of cereulide from an emetic b. cereus isolate (cau45) of raw milk were evaluated. the production of cereulide was tested by a cytotoxicity test and enzyme immunoassay, and confi ...201627275834
linking bacillus cereus genotypes and carbohydrate utilization capacity.we characterised carbohydrate utilisation of 20 newly sequenced bacillus cereus strains isolated from food products and food processing environments and two laboratory strains, b. cereus atcc 10987 and b. cereus atcc 14579. subsequently, genome sequences of these strains were analysed together with 11 additional b. cereus reference genomes to provide an overview of the different types of carbohydrate transporters and utilization systems found in b. cereus strains. the combined application of api ...201627272929
the impact of oxygen on bacterial enteric pathogens.bacterial enteric pathogens are responsible for a tremendous amount of foodborne illnesses every year through the consumption of contaminated food products. during their transit from contaminated food sources to the host gastrointestinal tract, these pathogens are exposed and must adapt to fluctuating oxygen levels to successfully colonize the host and cause diseases. however, the majority of enteric infection research has been conducted under aerobic conditions. to raise awareness of the import ...201627261784
elucidation of enterotoxigenic bacillus cereus outbreaks in austria by complementary epidemiological and microbiological investigations, 2013.identifying bacillus cereus as the causative agent of a foodborne outbreak still poses a challenge. we report on the epidemiological and microbiological investigation of three outbreaks of food poisoning (a, b, and c) in austria in 2013. a total of 44% among 32 hotel guests (a), 22% among 63 employees (b) and 29% among 362 residents of a rehab clinic (c) fell sick immediately after meal consumption. b. cereus isolated from left overs or retained samples from related foods were characterized by t ...201627257745
draft genome sequence of bacillus cereus 905, a plant growth-promoting rhizobacterium of wheat.bacillus cereus 905 is a plant growth-promoting rhizobacterium, isolated from wheat rhizosphere. the draft genome sequence of this strain is 5.39 mb and harbors 5,412 coding sequences.201627257205
draft whole-genome sequences of 11 bacillus cereus food isolates.bacillus cereus is a foodborne pathogen causing emetic and diarrheal-type syndromes. here, we report the whole-genome sequences of 11 b. cereus food isolates.201627257202
comparative bioinformatics and experimental analysis of the intergenic regulatory regions of bacillus cereus hbl and nhe enterotoxin operons and the impact of cody on virulence heterogeneity.bacillus cereus is a food contaminant with greatly varying enteropathogenic potential. almost all known strains harbor the genes for at least one of the three enterotoxins nhe, hbl, and cytk. while some strains show no cytotoxicity, others have caused outbreaks, in rare cases even with lethal outcome. the reason for these differences in cytotoxicity is unknown. to gain insight into the origin of enterotoxin expression heterogeneity in different strains, the architecture and role of 5' intergenic ...201627252687
probiotic bacillus cereus strains, a potential risk for public health in china.bacillus cereus is an important cause of foodborne infectious disease and food poisoning. however, b. cereus has also been used as a probiotic in human medicine and livestock production, with low standards of safety assessment. in this study, we evaluated the safety of 15 commercial probiotic b. cereus preparations from china in terms of mislabeling, toxin production, and transferable antimicrobial resistance. most preparations were incorrectly labeled, as they contained additional bacterial spe ...201627242738
contamination of healthcare workers' hands with bacterial spores.clostridium species and bacillus spp. are spore-forming bacteria that cause hospital infections. the spores from these bacteria are transmitted from patient to patient via healthcare workers' hands. although alcohol-based hand rubbing is an important hand hygiene practice, it is ineffective against bacterial spores. therefore, healthcare workers should wash their hands with soap when they are contaminated with spores. however, the extent of health care worker hand contamination remains unclear. ...201627236515
the role of active site flexible loops in catalysis and of zinc in conformational stability of bacillus cereus 569/h/9 β-lactamase.metallo-β-lactamases catalyze the hydrolysis of most β-lactam antibiotics and hence represent a major clinical concern. the development of inhibitors for these enzymes is complicated by the diversity and flexibility of their substrate-binding sites, motivating research into their structure and function. in this study, we examined the conformational properties of the bacillus cereus β-lactamase ii in the presence of chemical denaturants using a variety of biochemical and biophysical techniques. t ...201627235401
suitability of monotypic and mixed diets for anopheles hermsi larval development.the developmental time and survival to eclosion of anopheles hermsi barr & guptavanij fed monotypic and mixed diets of ten food types were examined in laboratory studies. larvae fed monotypic diets containing animal detritus (freeze-dried rotifers, freeze-dried daphnia pulicaria, and tetramin® fish food flakes) and the mixotrophic protistan cryptomonas ovata developed faster and survived better than larvae that were fed other monotypic diets. survival to adulthood of larvae fed several concentra ...201627232128
central venous access device-related bacillus cereus endocarditis: a case report and review of the literature.bacillus cereus typically presents as a gastrointestinal infection, but rarely manifests as systemic disease. this report describes a case of b. cereus-related endocarditis that presented as a sickle cell crisis and bacteremia. initial clinical suspicion was for laboratory contamination of blood cultures. the case herein described is intended to demonstrate an uncommon presentation of b. cereus infection and highlights the value of an aggressive need to further investigate and interpret unexpect ...201627231118
occurrence and behavior of bacillus cereus in naturally contaminated ricotta salata cheese during refrigerated storage.the present study shows the fate of bacillus cereus in refrigerated ricotta salata cheese during shelf-life. 144 ricotta salata cheese belonging to nine naturally contaminated batches were stored refrigerated and analyzed at 24 h, 30, 60 and 90 days of storage. total bacterial count, b. cereus spores and vegetative forms, intrinsic properties and composition were determined. the presence of spores was sporadic while the prevalence and the level of b. cereus vegetative cells decreased respectivel ...201627217369
destruction of bacillus cereus spores in a thick soy bean paste (doenjang) by continuous ohmic heating with five sequential electrodes.this study selected spores from bacillus cereus fsp-2 strain (the isolate from a commercial doenjang processing line) as the test strain which showed significantly higher thermal resistance (p < 0·05) than b. cereus reference strain (atcc 27348). the spores in doenjang were subjected to ohmic heating (oh) at 95, 105, 115 and 125°c for 30, 60 or 90 s using a five sequential electrode system (electrical field: 26·7 v cm(-1) ; alternating current frequency: 25 khz). oh at 105°c for 30-90 s reduced ...201627214292
detection and quantification of bacillus cereus group in milk by droplet digital pcr.droplet digital pcr (ddpcr) is one of the newest and most promising methods for the detection and quantification of molecular targets by pcr. here, we optimized and used a new ddpcr assay for the detection and quantification of the bacillus cereus group in milk. we also compared the ddpcr to a standard qpcr assay. the new ddpcr assay showed a similar coefficient of determination and a better limit of detection compared to the qpcr assay during quantification of the target molecules in the sample ...201627211508
three important amino acids control the regioselectivity of flavonoid glucosidation in glycosyltransferase-1 from bacillus cereus.glycosyltransferase-1 from bacillus cereus (bcgt1) catalyzes a reaction that transfers a glucosyl moiety to flavonoids, such as quercetin, kaempferol, and myricetin. the enzymatic glucosidation shows a broad substrate specificity when the reaction is catalyzed by wild-type bcgt1. preliminary assays demonstrated that the f240a mutant significantly improves the regioselectivity of enzymatic glucosidation toward quercetin. to unveil and further to control the catalytic function of bcgt1, mutation o ...201627198725
successful treatment of bacillus cereus bacteremia in a patient with propionic acidemia.bacillus cereus can cause serious, life-threatening, systemic infections in immunocompromised patients. the ability of microorganism to form biofilm on biomedical devices can be responsible for catheter-related bloodstream infections. other manifestations of severe disease are meningitis, endocarditis, osteomyelitis, and surgical and traumatic wound infections. the most common feature in true bacteremia caused by bacillus is the presence of an intravascular catheter. herein, we report a case of ...201627195164
characterization of bacillus cereus isolates from local dairy farms in china.bacillus cereus is an important opportunistic foodborne pathogen. in the present work, a total of 306 milk and environmental samples were collected from 10 local dairy farms in beijing, china. of the 92 b. cereus-like isolates, 88 and 4 belonged to b. cereus and b. thuringiensis, respectively. the prevalence of b. cereus isolates in bedding, feces, feed, liquid manure and raw milk was 93.3%, 78.9%, 41.2%, 100.0% and 9.8%, respectively. three main toxin genes nhe, hbl and ces were detected with r ...201627190168
characterization of lyspbc4, a novel bacillus cereus-specific endolysin of bacteriophage pbc4.bacillus cereus is a spore-forming, gram-positive bacterium and is a major food-borne pathogen. a b. cereus-specific bacteriophage pbc4 was isolated from the soil of a stock farm, and its genome was analyzed. pbc4 belongs to the siphoviridae family and has a genome consisting of 80 647-bp-long double-stranded dna, including 123 genes and two trnas. lyspbc4, the endolysin of pbc4, has an enzymatically active domain (ead) on its n-terminal region and a putative cell wall-binding domain (cbd) on it ...201627190165
the global regulator cody is required for the fitness of bacillus cereus in various laboratory media and certain beverages.the impact of gene mutations on the growth of the cells can be studied using pure cultures. however, the importance of certain proteins and pathways can be also examined via co-culturing wild type and its mutant derivative. here, the relative fitness of a mutant strain that lacks the global nitrogen regulator, cody, was examined in bacillus cereus, a food poisoning gram-positive bacterium. fitness measurements revealed that the δcody strain was outcompeted when cocultured with the wild-type atcc ...201627190142
the two-component signal transduction system yvcpq regulates the bacterial resistance to bacitracin in bacillus thuringiensis.yvcpq is one of the two-component signal transduction systems that respond to specific stimuli and enable cells to adjust multiple cellular functions. it consists of a histidine kinase yvcq and a response regulator yvcp. in this study, through searching the consensus sequence recognized by yvcp, we found four yvcp-binding motifs in the promoter regions of genes yvcr (bmb171_c4100), bmb171_c4385, kapd (bmb171_c4525) and bmb171_c4835 in bacillus thuringiensis bmb171 which is a representative of ba ...201627188294
label-free bimodal waveguide immunosensor for rapid diagnosis of bacterial infections in cirrhotic patients.spontaneous bacterial peritonitis is an acute bacterial infection of ascitic fluid; it has a high incidence in cirrhotic patients and it is associated with high mortality. in such a situation, early diagnosis and treatment is crucial for the survival of the patient. however, bacterial analysis in ascitic fluid is currently based on culture methods, which are time-consuming and laborious. we report here the application of a photonic interferometer biosensor based on a bimodal waveguide (bimw) for ...201627183281
coumarin derivatives as adjuvants: from in silico physicochemical characterization to in vitro evaluation against gram positive bacteria.a series of acylated coumarin derivatives have been evaluated for their in silico admet properties and in vitro antibacterial activities.201627151485
synthesis, anti-inflammatory and antibacterial activities of novel pyrazolo[4,3-g]pteridines.a novel series of 6-substituted pyrazolo[3,4-g]pteridines 2-9 and pyrazolo[4,3-e][1,2,4]triazolo[1,5-c]pteridin-2(3h)-one (thione) 10 and 11 was synthesized using the starting compound 3,7-dimethyl-1-phenylpyrazolo[4',3':5,6]pyrazino[2,3-d][1,3]oxazin-5(1h)-one 2. the structure of the newly synthesized compounds was elucidated by ir, (1)h-nmr, (13)c-nmr, mass spectroscopy and elemental analyses. the anti-inflammatory activity of all the newly synthesized compounds was evaluated using the carrage ...201627150479
pulmonary illness as a consequence of occupational exposure to shrimp shell powder.an employee with no prior history of allergy or asthma, experienced respiratory and flu-like symptoms during production of shrimp shell powder in a seafood savory factory in norway. we aimed to clarify the diagnosis and to identify the cause of the symptoms by specific inhalation challenge (sic) and by characterizing the powder's biocontaminants, particle size fractions and inflammatory potential.201627148672
in vitro evaluation of the antioxidant, 3,5-dihydroxy-4-ethyl-trans-stilbene (dets) isolated from bacillus cereus as a potent candidate against malignant melanoma.3,5-dihydroxy q1 -4-ethyl-trans-stilbene (dets) is a natural stilbene, which was first identified as bioactive bacterial secondary metabolite isolated from bacillus cereus associated with a rhabditid entomopathogenic nematode. the present study was intended to investigate the antioxidant and anticancer activity of this compound in vitro. antioxidant activity was investigated by assaying dpph free radical scavenging, superoxide radical-(o2..) scavenging, hydroxyl radical scavenging and metal chel ...201627148169
production of hemolysin bl by bacillus cereus group isolates of dairy origin is associated with whole-genome phylogenetic clade.bacillus cereus group isolates that produce diarrheal or emetic toxins are frequently isolated from raw milk and, in spore form, can survive pasteurization. several species within the b. cereus group are closely related and cannot be reliably differentiated by established taxonomical criteria. while b. cereus is traditionally recognized as the principal causative agent of foodborne disease in this group, there is a need to better understand the distribution and expression of different toxin and ...201627507015
degradation of acephate by enterobacter asburiae, bacillus cereus and pantoea agglomerans isolated from diamondback moth plutella xylostella (l), a pest of cruciferous crops.acephate-degrading bacterial isolates were isolated from the larval gut of diamondback moth plutella xylostella, a notorious pest of cruciferous crops worldwide that has developed resistance to insecticides. partial 16s rrna gene sequencing identified the isolates as bacillus cereus (px-b.c.or), enterobacter asburiae (pxe), and pantoae agglomerans (px-pt.ag.jor). all isolates grew on minimal media (mm) in the presence of acephate at 100 and 200 ppm, with maximum growth at 200 ppm. lc-ms analyses ...201627498509
comparative evaluation of cyanide removal by adsorption, biodegradation, and simultaneous adsorption and biodegradation (sab) process using bacillus cereus and almond shell.the present study aimed to investigate the removal efficiency of cyanide from contaminated water by adsorption, biodegradation and simultaneous adsorption and biodegradation (sab) process individually in a batch reactor. adsorption was achieved by using almond shell granules and biodegradation was conducted with suspended cultures of bacillus cereus, whereas sab process was carried out using bacillus cereus and almond shell in a batch reactor. the effect of agitation time, ph, and initial cyanid ...201627498500
simultaneous identification of 13 foodborne pathogens by using capillary electrophoresis-single strand conformation polymorphism coupled with multiplex ligation-dependent probe amplification and its application in foods.capillary electrophoresis-single strand conformation polymorphism (ce-sscp) coupled with stuffer-free multiplex ligation-dependent probe amplification (mlpa) was developed to identify 13 species of foodborne pathogens simultaneously. species-specific mlpa probes were designed for nine of these species. these probes were targeted to the groel, glya, mms, tuf, inv, ipah, nuc, vvh, and 16s rrna genes, which corresponded to bacillus cereus, campylobacter coli, cronobacter sakazakii, enterococcus spp ...201627494415
design, synthesis, anticancer, antimicrobial activities and molecular docking studies of theophylline containing acetylenes and theophylline containing 1,2,3-triazoles with variant nucleoside derivatives.a new series of theophylline containing acetylene derivatives (6a-6b and 7-13) and theophylline containing 1,2,3-triazoles with variant nucleoside derivatives (20-32) have been designed and synthesized. these compounds were screened for anticancer and antimicrobial activity. further the computational docking and 2d qsar were performed using moe software to identify novel scaffolds. the results showed that compound 29 and 30 exhibit significant cytotoxic effect on all four cancer cells such as lu ...201627487568
recovery of heat treated bacillus cereus spores is affected by matrix composition and factors with putative functions in damage repair.the ability of spores to recover and grow out after food processing is affected by cellular factors and by the outgrowth conditions. in the current communication we studied the recovery and outgrowth of individually sorted spores in bhi and rice broth media and on agar plates using flow cytometry. we show that recovery of wet heat treated bacillus cereus atcc 14579 spores is affected by matrix composition with highest recovery in bhi broth or on rice agar plates, compared to bhi agar plates and ...201627486443
hexane extract of echinops spinosissimus turra subsp. spinosus from tunisia: a potential source of acetylated sterols - investigation of its biological activities.the hexane extract of echinops spinosissimus turra subsp. spinosus flower heads was analyzed for its fatty acid and sterol composition. its physicochemical characteristics were also studied. the saponification, iodine and peroxide values were determined as 255 mg koh/g, 42.57 g i2 /100 g and 110 m equiv. o2 /kg of oil, respectively. the oleic (c18:1; 61.14%), palmitic (c16:0; 21.36%) and linoleic (c18:2; 10.45%) acids were the dominant fatty acids. this extract was also found to contain high lev ...201627476999
development of an efficient bacterial consortium for the potential remediation of hydrocarbons from contaminated sites.the intrinsic biodegradability of hydrocarbons and the distribution of proficient degrading microorganisms in the environment are very crucial for the implementation of bioremediation practices. among others, one of the most favorable methods that can enhance the effectiveness of bioremediation of hydrocarbon-contaminated environment is the application of biosurfactant producing microbes. in the present study, the biodegradation capacities of native bacterial consortia toward total petroleum hyd ...201627471499
myrcia ovata cambessedes essential oils: a proposal for a novel natural antimicrobial against foodborne bacteria.this paper reports the innovative antibacterial activity of essential oils (eos) from nine myrcia ovata cambessedes plants against eight foodborne bacteria. staphylococcus aureus, bacillus cereus, bacillus subtilis, enterococcus faecalis and pseudomonas aeruginosa were the most susceptible bacteria to eos. in particular, the p. aeruginosa, which is usually resistant to antimicrobials agents, was extremely sensitive to some eos. the gram-positive and gram-negative bacteria were inhibited and elim ...201627554278
proteome data to explore the impact of pbclin15 on bacillus cereus atcc 14579.this data article reports changes in the cellular and exoproteome of b. cereus cured from pbclin15.time-course changes of proteins were assessed by high-throughput nanolc-ms/ms. we report all the peptides and proteins identified and quantified in b. cereus with and without pbclin15. proteins were classified into functional groups using the information available in the kegg classification and we reported their abundance in term of normalized spectral abundance factor. the repertoire of experiment ...201627547804
new insights into the interaction between the quorum-sensing receptor nprr and its dna target, or the response regulator spo0f.the nprr protein and nprrb signaling peptide comprise a bifunctional quorum-sensing system from the bacillus cereus group that is involved in transcriptional activation through dna-binding and in sporulation initiation by binding to spo0f. we characterized in vitro the direct interactions established by nprr that may be relevant for performing its two functions. apo-nprr interacted with spo0f, but not with the target dna. the nprrb signaling peptide sskpdivg that binds strongly to apo-nprr, fail ...201627543719
persistent bacillus cereus bacteremia in 3 persons who inject drugs, san diego, california, usa.bacillus cereus is typically considered a blood culture contaminant; however, its presence in blood cultures can indicate true bacteremia. we report 4 episodes of b. cereus bacteremia in 3 persons who inject drugs. multilocus sequence typing showed that the temporally associated infections were caused by unrelated clones.201627533890
mechanism of enhanced antibacterial activity of ultra-fine zno in phosphate buffer solution with various organic acids.ultra-fine-zno showed low toxicity in complex water matrix containing multiple components such as pbs buffer and the toxic mechanism of ultra-fine-zno has not been clearly elucidated. in present study, enhanced antibacterial activity of 200 nm diameter ultra-fine-zno in pbs buffer against bacillus cereus and escherichia coli were observed in the presence of several organic acids in comparison with ultra-fine-zno in pbs buffer alone. these findings indicated that the toxic effects of the ultra-fi ...201627524254
bacillus wiedmannii sp. nov., a psychrotolerant and cytotoxic bacillus cereus group species isolated from dairy foods and dairy environments.a facultatively anaerobic, spore-forming bacillus strain, fsl w8-0169t, collected from raw milk stored in a silo at a dairy powder processing plant in the north-eastern usa was initially identified as a bacillus cereus group species based on a partial sequence of the rpob gene and 16s rrna gene sequence. analysis of core genome single nucleotide polymorphisms clustered this strain separately from known b. cereus group species. pairwise average nucleotide identity blast values obtained for fsl w8 ...201627520992
potential risk of acute toxicity induced by agi cloud seeding on soil and freshwater biota.silver iodide is one of the most common nucleating materials used in cloud seeding. previous cloud seeding studies have concluded that agi is not practically bioavailable in the environment but instead remains in soils and sediments such that the free ag amounts are likely too low to induce a toxicological effect. however, none of these studies has considered the continued use of this practice on the same geographical areas and thus the potential cumulative effect of environmental agi. the aim o ...201627517140
possession, use, and transfer of select agents and toxins-- addition of bacillus cereus biovar anthracis to the hhs list of select agents and toxins. interim final rule and request for comments.the centers for disease control and prevention (cdc) in the department of health and human services (hhs) is adding bacillus cereus biovar anthracis to the list of hhs select agents and toxins as a tier 1 select agent. we are taking this action to regulate this agent that is similar to b. anthracis to prevent its misuse, which could cause a biological threat to public health and/or national security.201627632805
differentiation of foodborne bacteria using nir hyperspectral imaging and multivariate data analysis.the potential for near-infrared (nir) hyperspectral imaging and multivariate data analysis to be used as a rapid non-destructive tool for detection and differentiation of bacteria was investigated. nir hyperspectral images were collected of bacillus cereus, escherichia coli, salmonella enteritidis, staphylococcus aureus and staphylococcus epidermidis grown on agar for 20 h at 37 °c. principal component analysis (pca) was applied to mean-centred data. standard normal variate (snv) correction and ...201627624097
termitarium-inhabiting bacillus endophyticus tsh42 and bacillus cereus tsh77 colonizing curcuma longa l.: isolation, characterization, and evaluation of their biocontrol and plant-growth-promoting activities.bacillus strains were isolated from termitarium soil and screened for their antifungal activity through the production of diffusible and volatile metabolites. further, the bacterial strains that showed antifungal activity were evaluated for their biocontrol potential on the basis of their plant-growth-promoting attributes. termitarium-inhabiting bacillus strains tsh42 and tsh77 significantly reduced the growth of pathogenic fungus fusarium solani, controlled the symptoms of rhizome rot in turmer ...201627604298
the comparative analysis of antioxidant and biological activity for the dendropanax morbifera lev. leaves extracted by different ethanol concentrations.the different concentrations of ethanol (20-100%) and distilled water extract for dendropanax morbifera lev. leaves were evaluated to induce antioxidant and biological activity employed by variety of assays. the 20%, 80%, and 100% ethanol extract demonstrated the relatively higher activity, whereas distilled water, 40%, and 60% ethanol extracts exhibited the lower antioxidant and biological activity. especially, 80% ethanol extract showed the remarkably higher radical scavenging activity, reduci ...201627592831
genotoxicity evaluation of tannery effluent treated with newly isolated hexavalent chromium reducing bacillus cereus.in this study, the efficiency of free and immobilized cells of newly isolated hexavalent chromium [cr(vi)] reducing bacillus cereus strain cr1 (accession no. kj162160) was studied in the treatment of tannery effluent. the analysis of effluents revealed high chemical oxygen demand (cod-1260 mg/l), biological oxygen demand (bod5-660 mg/l), total dissolved solids (tds-14000 mg/l), electrical conductivity (ec-21.5 ms/cm) and total chromium (tc-2.4 mg/l). the effluents also showed genotoxic effects t ...201627591849
draft genome sequence of biocontrol agent bacillus cereus uw85.bacillus cereus uw85 was isolated from a root of a field-grown alfalfa plant from arlington, wi, and identified for its ability to suppress damping off, a disease caused by phytophthora megasperma f. sp. medicaginis on alfalfa. here, we report the draft genome sequence of b. cereus uw85, obtained by a combination of sanger and illumina sequencing.201627587823
bacterial succession and metabolite changes during flax (linum usitatissimum l.) retting with bacillus cereus hdym-02.high-throughput sequencing and gc-ms (gas chromatography-mass spectrometry) were jointly used to reveal the bacterial succession and metabolite changes during flax (linum usitatissimum l.) retting. the inoculation of bacillus cereus hdym-02 decreased bacterial richness and diversity. this inoculum led to the replacement of enterobacteriaceae by bacillaceae. the level of aerobic pseudomonadaceae (mainly azotobacter) and anaerobic clostridiaceae_1 gradually increased and decreased, respectively. f ...201627585559
the comparing of antimicrobial activity of csn1s2 protein of fresh milk and yoghurt goat breed ethawah inhibited the pathogenic bacteria.goat milk is reported to have antimicrobial activity of several pathogen bacteria that contained on food materials. the research related with antimicrobial activity of alpha-s2 casein from goat milk is relatively less than other casein components. herein, we reported the antimicrobial activity of caprine alpha-s2 casein (csn1s2) protein from ethawah breed goat milk and yoghurt in gram positive (listeria monocytogenes, staphylococcus aureus and bacillus cereus) and negative pathogen bacteria (esc ...201627698594
draft genome sequence of bacillus cereus lcr12, a plant growth-promoting rhizobacterium isolated from a heavy metal-contaminated environment.bacillus cereus lcr12 is a plant growth-promoting rhizobacterium, isolated from a heavy metal-contaminated environment. the 6.01-mb annotated genome sequence provides the genetic basis for revealing its potential application to remediate contaminated soils in association with plants.201627688340
genome sequence of bacillus cereus group phage salinjah.the double-stranded dna (dsdna) myoviridae bacillus cereus group bacteriophage salinjah was isolated from soil collected in gyeonggi-do, south korea. salinjah, a cluster c phage with a broad host range, suggests the need to create a new subcluster with salinjah and helga as founding members.201627688335
bioactivity of a novel glycolipid produced by a halophilic buttiauxella sp. and improving submerged fermentation using a response surface method.an antimicrobial glycolipid biosurfactant (gbs), extracted and identified from a marine bacterium, was studied to inhibit pathogenic microorganisms. production of the gbs was optimized using a statistical method, a response surface method (rsm) with a central composite design (ccd) for obtaining maximum yields on a cost-effective substrate, molasses. the gbs-producing bacterium was identified as buttiauxella species in terms of biochemical and molecular characteristics. this compound showed a de ...201627669197
commercial disinfectants during disinfection process validation: more failures than success.disinfection process validation is mandatory before introduction of a new disinfectant in hospital services. commercial disinfection brands often question existing hospital policy claiming greater efficacy and lack of toxicity of their products. inadvertent inadequate disinfection leads to morbidity, patient's economic burden, and the risk of mortality.201627656441
chemical composition and in vitro antimicrobial, cytotoxic, and central nervous system activities of the essential oils of citrus medica l. cv. 'liscia' and c. medica cv. 'rugosa' cultivated in southern italy.citrus medica cv. 'liscia' and c. medica cv. 'rugosa' are two taxa of citron, belonging to the biodiversity of south italy, in particular of amalfi coast, in the campania region. the chemical composition of the essential oils (eos) from fruit peels of both c. medica cultivars was studied by gas chromatography (gc) and gas chromatography-mass spectrometry (gc-ms) analyses. in all, 100 compounds were identified, 82 for c. medica cv. 'liscia', accounting for 91.4% of the total oil, and 88 for c. me ...201627649138
chemical composition, antibacterial and phytotoxic activities of peganum harmala seed essential oils from five different localities in northern africa.peganum harmala l., also known as syrian rue or pègano, is a herbaceous plant belonging to the zygohpyllaceae family, and is widely used in traditional medicine. the chemical composition of essential oils of p. harmala seeds from five different regions of northern africa (algeria, egypt, libya, morocco and tunisia) was studied by gc and gc-ms analyses. a total of 105 compounds were identified, the main components being oxygenated monoterpenes and oxygenated sesquiterpenes. eugenol is the main co ...201627649128
pellino-1 derived cationic antimicrobial prawn peptide: bactericidal activity, toxicity and mode of action.the antimicrobial peptides (amps) are multifunctional molecules which represent significant roles in the innate immune system. these molecules have been well known for decades because of their role as natural antibiotics in both invertebrates and vertebrates. the development of multiple drug resistance against conventional antibiotics brought a greater focus on amps in recent years. the cationic peptides, in particular, proven as host defense peptides and are considered as effectors of innate im ...201627648859
genome sequence of bacillus cereus forc_021, a food-borne pathogen isolated from a knife at a sashimi restaurant.bacillus cereus causes food-borne illness through contaminated foods; therefore, its pathogenicity and genome sequences have been analyzed in several studies. we sequenced and analyzed b. cereus strain forc_021 isolated from a sashimi restaurant. the genome sequence consists of 5,373,294 bp with 35.36% gc contents, 5,350 predicted cdss, 42 rrna genes, and 107 trna genes. based on in silico dna-dna hybridization values, b. cereus atcc 14579(t) was closest to forc_021 among the complete genome-seq ...201627728961
feasible management of southern corn leaf blight via induction of systemic resistance by bacillus cereus c1l in combination with reduced use of dithiocarbamate fungicides.dithiocarbamate fungicides such as maneb and mancozeb are widely used nonsystemic protectant fungicides to control various plant fungal diseases. dithiocarbamate fungicides should be frequently applied to achieve optimal efficacy of disease control and avoid either decline in effectiveness or wash-off from leaf surface. dithiocarbamates are of low resistance risk but have the potential to cause human neurological diseases. the objective of this study was to develop a strategy to effectively cont ...201627721698
chemical variability and biological activities of essential oils of micromeria inodora (desf.) benth. from algeria.the chemical composition of the essential oils isolated from the aerial parts of micromeria inodora (desf.) benth. collected in 24 algerian localities was investigated from the first time using gc-fid, gc/ms and (13) c-nmr. altogether, 83 components which accounted for 94.7% of the total oil composition were identified. the main compounds were trans-sesquisabinene hydrate (1; 20.9%), α-terpinyl acetate (2; 19.8%), globulol (3; 4.9%), caryophyllene oxide (4; 4.3%), β-bisabolol (5; 2.9%) and trans ...201627448034
application of volcanic ash particles for protein affinity purification with a minimized silica-binding tag.we recently reported that the spore coat protein, cotb1 (171 amino acids), from bacillus cereus mediates silica biomineralization and that the polycationic c-terminal sequence of cotb1 (14 amino acids), designated cotb1p, serves as a silica-binding tag when fused to other proteins. here, we reduced the length of this silica-binding tag to only seven amino acids (sb7 tag: rqssrgr) while retaining its affinity for silica. alanine scanning mutagenesis indicated that the three arginine residues in t ...201627212265
biosynthesis of palladium nanoparticles by using moringa oleifera flower extract and their catalytic and biological properties.the biosynthesis of nanostructured biopalladium nanoparticles (pdnps) from an aqueous solution of crystalline palladium acetate is reported. for the synthesised pdnps in solution, an agroforest biomass waste petal of moringa oleifera derived bis-phthalate was used as natural reducing and biocapping agents. continuous absorption in the uv region and subsequent brown colour change confirmed the formation of pdnps. a strong surface plasmon peak for pdnps occurred at 460nm. pdnps were characterized ...201627776261
cotranscriptional folding of a riboswitch at nucleotide resolution.rnas can begin to fold immediately as they emerge from rna polymerase. during cotranscriptional folding, interactions between nascent rnas and ligands are able to direct the formation of alternative rna structures, a feature exploited by noncoding rnas called riboswitches to make gene-regulatory decisions. despite their importance, cotranscriptional folding pathways have yet to be uncovered with sufficient resolution to reveal how cotranscriptional folding governs rna structure and function. to ...201627798597
spovt: from fine-tuning regulator in bacillus subtilis to essential sporulation protein in bacillus cereus.sporulation is a highly sophisticated developmental process adopted by most bacilli as a survival strategy to withstand extreme conditions that normally do not support microbial growth. a complicated regulatory cascade, divided into various stages and taking place in two different compartments of the cell, involves a number of primary and secondary regulator proteins that drive gene expression directed toward the formation and maturation of an endospore. such regulator proteins are highly conser ...201627790204
new benzophenones and xanthones from cratoxylum sumatranum ssp. neriifolium and their antibacterial and antioxidant activities.two new benzophenones (1 and 2) and four new xanthones (4-6 and 17) together with 24 known compounds (3, 7-16, and 18-30) were isolated from the roots and twigs of cratoxylum sumatranum ssp. neriifolium. their structures were elucidated by spectroscopic methods. compounds 5 and 26 showed antibacterial activity against micrococcus luteus, bacillus cereus, and staphylococcus epidermis with minimum inhibitory concentrations ranging from 4 to 8 μg/ml, whereas compounds 7, 20, and 26 displayed select ...201627788582
use of molecular typing to investigate bacterial translocation from the intestinal tract of chlorpyrifos-exposed rats.human are confronted on a daily basis with contaminant pesticide residues in food, water and other components of the environment. although the digestive system is the first organ to come into contact with food contaminants, very few data are available on the impact of low-dose pesticide exposure during the in utero and postnatal periods on intestinal bacterial translocation (bt). previous studies have revealed that chlorpyrifos (cpf) exposure is associated with intestinal dysbiosis and the conta ...201627826358
optimization of the production conditions of the lipase produced by bacillus cereus from rice flour through plackett-burman design (pbd) and response surface methodology (rsm).in this study, the screening of lipase positive bacteria from rice flour was carried out by rhodamin b agar plate method. bacillus cereus was identified by 16s rdna method. screening of the appropriate variables and optimization of the lipase production was performed using plackett-burman design (pbd) and response surface methodology (rsm). among the isolated bacteria, an aerobic bacillus cereus strain was recognized as the best lipase-producing bacteria (177.3 ± 20 u/ml). given the results, the ...201627816679
the large-scale process of microbial carbonate precipitation for nickel remediation from an industrial soil.microbial carbonate precipitation is known as an efficient process for the remediation of heavy metals from contaminated soils. in the present study, a urease positive bacterial isolate, identified as bacillus cereus ns4 through 16s rdna sequencing, was utilized on a large scale to remove nickel from industrial soil contaminated by the battery industry. the soil was highly contaminated with an initial total nickel concentration of approximately 900 mg kg(-1). the soluble-exchangeable fraction wa ...201627814530
elucidating the rimosamide-detoxin natural product families and their biosynthesis using metabolite/gene cluster correlations.as microbial genome sequencing becomes more widespread, the capacity of microorganisms to produce an immense number of metabolites has come into better view. utilizing a metabolite/gene cluster correlation platform, the biosynthetic origins of a new family of natural products, the rimosamides, were discovered. the rimosamides were identified in streptomyces rimosus and associated with their nrps/pks-type gene cluster based upon their high frequency of co-occurrence across 179 strains of actinoba ...201627809474
Refresh
firstprev
12345678910...
nextlast
Displaying items 401 - 500 of 8187