Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| draft genome sequence of enterococcus faecalis atcc baa-2128. | while a part of the native gut microflora, the gram-positive bacterium enterococcus faecalis can lead to serious infections elsewhere in the body. the draft genome of e. faecalis strain atcc baa-2128, isolated from piglet feces, was examined. this draft genome consists of 42 contigs, 12 of which exhibit homology to annotated plasmids. | 2017 | 28684576 |
| role of purified β-1, 3 glucan binding protein (β-gbp) from paratelphusa hydrodromus and their anti-inflammatory, antioxidant and antibiofilm properties. | β- 1, 3-glucan binding protein (β-gbp), a pattern recognition protein (prp), plays a critical role in triggering the innate immune response by detecting β-glucan found on the surface of microbes. in the present study, β-gbp was purified from the haemolymph of rice field crab paratelphusa hydrodromus by affinity column chromatography. the monomeric protein ph-β-gbp appeared as a single band with a molecular weight of approximately 95 kda in sds-page analysis and its purity was determined to be 89 ... | 2017 | 28684323 |
| incidence, clinical characteristics, and outcomes of nosocomial enterococcus spp. bloodstream infections in a tertiary-care hospital in beijing, china: a four-year retrospective study. | enterococcus spp. are the common cause of nosocomial bloodstream infections (bsis) with high morbidity and mortality. the purpose of this study was to characterize the incidence, clinical and microbiological features, and mortality of nosocomial enterococcal bsis at a large chinese tertiary-care hospital in beijing, china. | 2017 | 28680588 |
| the enterococcus faecalis virulence factor elra interacts with the human four-and-a-half lim domains protein 2. | the commensal bacterium enterococcus faecalis is a common cause of nosocomial infections worldwide. the increasing prevalence of multi-antibiotic resistant e. faecalis strains reinforces this public health concern. despite numerous studies highlighting several pathology-related genetic traits, the molecular mechanisms of e. faecalis virulence remain poorly understood. in this work, we studied 23 bacterial proteins that could be considered as virulence factors or involved in the enterococcus inte ... | 2017 | 28676674 |
| stochasticity in the enterococcal sex pheromone response revealed by quantitative analysis of transcription in single cells. | in enterococcus faecalis, sex pheromone-mediated transfer of antibiotic resistance plasmids can occur under unfavorable conditions, for example, when inducing pheromone concentrations are low and inhibiting pheromone concentrations are high. to better understand this paradox, we adapted fluorescence in situ hybridization chain reaction (hcr) methodology for simultaneous quantification of multiple e. faecalis transcripts at the single cell level. we present direct evidence for variability in the ... | 2017 | 28671948 |
| carriage of multidrug resistant enterococcus faecium and enterococcus faecalis among apparently healthy humans. | enterococci are indigenous flora of the gastro-intestinal tracts of animals and humans. recently, interest in two major species, e. faecium and e. faecalis, has heightened because of their ability to cause serious infections and their intrinsic resistance to antimicrobials. this study was aimed at determining the prevalence of e. faecium and e. faecalis in human faecal samples and evaluating the susceptibility of the isolates to antibiotics. | 2017 | 28670644 |
| prevalence and genetic diversity of enterococcus faecalis isolates from mineral water and spring water in china. | enterococcus faecalis is an important opportunistic pathogen which is frequently detected in mineral water and spring water for human consumption and causes human urinary tract infections, endocarditis and neonatal sepsis. the aim of this study was to determine the prevalence, virulence genes, antimicrobial resistance and genetic diversity of e. faecalis from mineral water and spring water in china. of 314 water samples collected from january 2013 to january 2014, 48 samples (15.3%) were contami ... | 2017 | 28670302 |
| determining the optimal dose of 1940-nm thulium fiber laser for assisting the endodontic treatment. | insufficient cleaning, the complex anatomy of the root canal system, inaccessible accessory canals, and inadequate penetration of irrigants through dentinal tubules minimizes the success of the conventional endodontic treatment. laser-assisted endodontic treatment enhances the quality of conventional treatment, but each laser wavelength has its own its own limitations. the optimal parameters for the antibacterial efficiency of a new wavelength, 1940-nm thulium fiber laser, were firstly investiga ... | 2017 | 28669067 |
| urinary tract infections after kidney transplantation: a risk factor analysis of 417 patients. | background the aim of this study was to evaluate the number of urinary tract infections (uti) that occur after kidney transplantation (kt) and to identify possible risk factors for development of a uti. material and methods we retrospectively analyzed all kts performed between january 2012 and december 2013 in the erasmus university medical center, rotterdam. uti was scored if: (1) a patient had a urine culture with no more than 2 species of microorganisms, (2) at least 1 of which was a bacteriu ... | 2017 | 28663538 |
| altered antibacterial activity of curcumin in the presence of serum albumin, plasma and whole blood. | antibacterial effect is one of the major therapeutic activities of plant-derived curcumin. this work evaluated the effect of serum albumin, human plasma, and whole blood on the in vitro activity of curcumin against eight clinical bacterial isolates by standard broth microdilution and plate-counting methods. toxicological effects of curcumin towards human red blood cells (rbcs) and peripheral blood mononuclear cells (pbmcs) were also investigated. curcumin exhibited weak activity against gram-neg ... | 2017 | 28649069 |
| [mitral valve endocarditis diagnosed after panophthalmitis with enterococcus faecalis]. | an 86-year-old man underwent cataract surgery on the left eye, but suddenly he lost his vision three days after surgery. topical treatments for panophthalmitis were started according to international guidelines. later, a broad-range polymerase chain reaction showed enterococcus faecalis in corpus vitreum, and iv antiobiotic treatment was initiated. to exclude endocarditis an echocardiography was performed, and this showed mitral regurgitation with a small mobile vegetation. uncertainty concernin ... | 2017 | 28648168 |
| antibacterial effect of genetically-engineered bacteriophage ϕef11/ϕfl1c(δ36)p(nisa) on dentin infected with antibiotic-resistant enterococcus faecalis. | enterococcus faecalis is a gram-positive facultative anaerobic bacterium, which is present in 30-89% of teeth with postendodontic treatment failures. e. faecalis is capable of penetrating dentinal tubules and surviving as a monoculture after conventional endodontic therapy, indicating that it is resistant to commonly used endodontic disinfection protocols. different e. faecalis strains have shown resistance to several antibiotics, and have been associated with both dental pathology and systemic ... | 2017 | 28646693 |
| phtβ-promoted mobilization of non-conjugative resistance plasmids from enterococcus faecium to enterococcus faecalis. | to analyse the recombination events associated with conjugal mobilization of two multiresistance plasmids, prum17i48 and plag (formerly named pdo1-like), from enterococcus faecium 17i48 to enterococcus faecalis jh2-2. | 2017 | 28645197 |
| methacrylate-based root canal sealer containing chlorexidine and α-tricalcium phosphate. | the aim of this study was to develop and to characterize a methacrylate-based root canal sealer containing chlorhexidine (chx) and α-tricalcium phosphate (α-tcp). experimental dual cure methacrylate-based sealer was produced containing 0, 2.5, or 5 wt% of chx and 0, 25, or 50 wt% of α-tcp. experimental sealers were evaluated based on flow, film thickness, radiopacity, degree of conversion (dc), degradation in water, ph and antibacterial activity. flow ranged from 15.09 ± 0.11 to 17.47 ± 0.42 mm. ... | 2017 | 28644564 |
| exploiting crispr-cas to manipulate enterococcus faecalis populations. | crispr-cas provides a barrier to horizontal gene transfer in prokaryotes. it was previously observed that functional crispr-cas systems are absent from multidrug-resistant (mdr) enterococcus faecalis, which only possess an orphan crispr locus, termed crispr2, lacking cas genes. here, we investigate how the interplay between crispr-cas genome defense and antibiotic selection for mobile genetic elements shapes in vitro e. faecalis populations. we demonstrate that crispr2 can be reactivated for gen ... | 2017 | 28644125 |
| comparative evaluation of anti microbial effects of triple antibiotic paste and amox and its derivatives against e. faecalis: an in vitro study. | enterococcus faecalis is a microorganism commonly detected in asymptomatic, persistent endodontic infections. triple antibiotic paste has stood the test of time as a proven antibiotic combination against e. faecalis. however, problems with this include staining of teeth and standardization of the preparation. thus, the search for better alternatives and better preparation techniques is still on. | 2017 | 28638559 |
| antibacterial activity of different root canal sealers against enterococcus faecalis. | the aim of the present study was to compare in vitro the antimicrobial activity of different root canal sealers against enterococcus faecalis, prior and subsequent to setting. | 2017 | 28638549 |
| telavancin: a novel semisynthetic lipoglycopeptide agent to counter the challenge of resistant gram-positive pathogens. | telavancin (td-6424), a semisynthetic lipoglycopeptide vancomycin-derivative, is a novel antimicrobial agent developed by theravance for overcoming resistant gram-positive bacterial infections, specifically methicillin-resistant staphylococcus aureus (mrsa). the us food and drug administration (usfda) had approved telavancin in 2009 for the treatment of complicated skin and skin structure infections (csssis) caused by gram-positive bacteria, including mrsa (s. aureus, streptococcus agalactiae, s ... | 2017 | 28634536 |
| antibacterial activity of crude extracts of some south african medicinal plants against multidrug resistant etiological agents of diarrhoea. | this study evaluated the antibacterial activity of some plants used in folklore medicine to treat diarrhoea in the eastern cape province, south africa. | 2017 | 28629407 |
| molecular identification of multiple antibiotic resistant fish pathogenic enterococcus faecalis and their control by medicinal herbs. | the opportunistic fish pathogen, enterococcus faecalis has been reported to cause mass mortality in several fish species in different countries. the objectives of this study were to (i) identify e. faecalis from the diseased fishes through molecular techniques; (ii) assess the antibiotic susceptibility profile of e. faecalis isolates; and (iii) control disease in tilapia fish by treatment with medicinal plant extracts. a total of 48 isolates were phenotypically identified as enterococcus species ... | 2017 | 28623336 |
| bactericidal effect of er:yag laser-activated sodium hypochlorite irrigation against biofilms of enterococcus faecalis isolate from canal of root-filled teeth with periapical lesions. | this study was to evaluate the bactericidal effect of er:yag laser-activated sodium hypochlorite irrigation (er:yag + naocl) on biofilms of enterococcus faecalis clinical isolate. | 2017 | 28622484 |
| design, synthesis, and evaluation of novel tyrosine-based dna gyrase b inhibitors. | the discovery and synthesis of new tyrosine-based inhibitors of dna gyrase b (gyrb), which target its atpase subunit, is reported. twenty-four compounds were synthesized and evaluated for activity against dna gyrase and dna topoisomerase iv. the antibacterial properties of selected gyrb inhibitors were demonstrated by their activity against staphylococcus aureus and enterococcus faecalis in the low micromolar range. the most promising compounds, 8a and 13e, inhibited escherichia coli and s. aure ... | 2017 | 28621824 |
| spectrum and antibiotic resistance of uropathogens between 2004 and 2015 in a tertiary care hospital in hungary. | surveillance of the bacterial spectrum and antibiotic-resistance patterns of locally occurring uropathogens is essential to serve as a basis for empirical treatment of urinary tract infections (utis), as antibiotic-resistance rates may vary geographically with significant differences between countries and regions, and with time. | 2017 | 28621642 |
| photodvnamic therapy in endodontics. | commonly used irrigants do not always eradicate the entire microbial flora in infected root canals. therefore, several other strategies, such as photodynamic therapy (pdt) have been developed. photoactivated disinfection is based on the interaction of a photosensitive antibacterial agent and a light source. it uses a nontoxic dye named photosensitizer (ps) and low-intensity visible light. in oxygen presentation, these combine to produce some cytotoxic species. the ps molecules attach to bacteria ... | 2017 | 28621288 |
| infectious crystalline keratopathy after descemet’s stripping endothelial keratoplasty. | a 68-year-old woman presented with infectious crystalline keratopathy 4 months after she underwent a combined phacoemulsification and descemet's stripping endothelial keratoplasty for fuch's endothelial dystrophy in her left eye. after 5 months of topical moxifloxacin 1%, the infiltrate responded well but had not completely resolved, with the resulting endothelial failure requiring a penetrating keratoplasty 9 months after the initial operation. microbiology identified enterococcus faecalis with ... | 2017 | 28619977 |
| draft genome sequences of six enterococcus faecalis strains isolated from malaysian clinical and environmental origins. | enterococcus faecalis is known to cause a variety of nosocomial infections, including urinary tract infections. antibiotic resistance and virulence properties in this species are of public concern. the draft genome sequences of six e. faecalis strains isolated from clinical and environmental sources in malaysia are presented here. | 2017 | 28619812 |
| evaluation of antimicrobial activity of association of chlorhexidine to photosensitizer used in photodynamic therapy in root canals infected by enterococcus faecalis. | the aim of the present study was to evaluate, in vitro, the influence of the addition of chlorhexidine to photosensitiser in the antimicrobial activity of photodynamic therapy in root canals infected by enterococcus faecalis. | 2017 | 28619615 |
| triterpenoid saponins and other glycosides from the stems and bark of jaffrea xerocarpa and their biological activity. | six previously undescribed triterpenoid saponins and two previously undescribed norlupane triterpenes were isolated with five known saponins, three known lupane derivatives, 17,20-didehydro-20-deoxyjujubogenin, rutin, (±) 3α-o-β-d-glucopyranosyl-lyoniresinol, (±) 4-o-β-d-glucopyranosyl-maesopsin, three phenol glycosides, and uridine from the stems and bark of jaffrea xerocarpa (baill.) h. c. hopkins & pillon (= basionym alphitonia xerocarpus baill.) (rhamnaceae), an endemic tree of new caledonia ... | 2017 | 28614728 |
| signal biosynthesis inhibition with ambuic acid as a strategy to target antibiotic-resistant infections. | there has been major interest by the scientific community in antivirulence approaches against bacterial infections. however, partly due to a lack of viable lead compounds, antivirulence therapeutics have yet to reach the clinic. here we investigate the development of an antivirulence lead targeting quorum sensing signal biosynthesis, a process that is conserved in gram-positive bacterial pathogens. some preliminary studies suggest that the small molecule ambuic acid is a signal biosynthesis inhi ... | 2017 | 28607020 |
| bacterial sepsis in neonates: single centre study in a neonatal intensive care unit in bosnia and herzegovina. | the aim of the study was to evaluate the incidence, mortality, risk factors, aetiology and the susceptibility to antibiotics of the bacteria responsible for sepsis. | 2017 | 28605923 |
| a pilot-scale microwave technology for sludge sanitization and drying. | large volumes of sludge are produced from onsite sanitation systems in densely populated areas (e.g. slums and emergency settlements) and wastewater treatment facilities that contain high amounts of pathogens. there is a need for technological options which can effectively treat the rapidly accumulating sludge under these conditions. this study explored a pilot-scale microwave (mw) based reactor as a possible alternative for rapid sludge treatment. the reactor performance was examined by conduct ... | 2017 | 28605862 |
| molecular epidemiology and antibiotic resistance patterns of enterococcus faecalis isolates from hospitals in tehran. | enterococcus faecalis is one of the most important causative agents of nosocomial infections. control and prevention of these infections require appropriate epidemiological knowledge. this study investigated the correlation between molecular characteristics and drug resistance of e. faecalis isolates from local hospitals. a total of 125 isolates of enterococcus faecalis from two hospitals in tehran were identified by using culture and biochemical method. an antibiotic resistance assay was carrie ... | 2017 | 28603229 |
| a new epipolythiodioxopiperazine with antibacterial and cytotoxic activities from the endophytic fungus chaetomium sp. m336. | a new compound 6-formamide-chetomin (1) together with chetomin (2) was isolated from solid fermentation products of the endophytic fungus chaetomium sp. m336, which were elucidated by extensive spectroscopic analyses, including 1d and 2d nmr, and hr-esi-ms experiments. the bioassay result showed that compound 1 had strong antibacterial activity against escherichia coli, staphylococcus aureus, salmonella typhimurium atcc 6539 and enterococcus faecalis with minimum inhibitory concentration (mic) v ... | 2017 | 28602097 |
| antibiotic resistance genes and antibiotic susceptibility of oral enterococcus faecalis isolates compared to isolates from hospitalized patients and food. | enterococcus faecalis, a commensal of the intestinal tract of humans and animals is of great significance as leading opportunistic pathogen, and also prevalent in oral diseases, such as endodontic infections, as well as the healthy oral cavity. to investigate the potential of oral e. faecalis to constitute a reservoir of antibiotic resistance, isolates from supragingival plaque/saliva and from endodontic infections were screened regarding their resistance to selected antibiotics in comparison to ... | 2017 | 28601926 |
| a loop-mediated isothermal amplification (lamp) assay for the rapid detection of enterococcus spp. in water. | faecal pollution of water and the resulting potential presence of human enteric pathogens is a predominant threat to public health. microbiological water quality can be assessed by the detection of standard faecal indicator bacteria (sfib) such as e. coli or certain enterococcus species. in recent years, isothermal amplification methods have become a useful alternative to polymerase chain reaction (pcr), allowing molecular diagnostics with simple or no instrumentation. in this study, a novel scr ... | 2017 | 28591662 |
| an entirely solid phase peptide synthesis-based strategy for synthesis of gelatinase biosynthesis-activating pheromone (gbap) analogue libraries: investigating the structure-activity relationships of the enterococcus faecalis quorum sensing signal. | the development of an entirely solid-phase peptide synthesis (spps)-based synthesis of the quorum sensing signal gelatinase biosynthesis-activating pheromone (gbap) from enterococcus faecalis is reported. the method was used to prepare three libraries of analogues to investigate the structure-activity relationships (sars) of the gbap signal. the sar studies revealed new characteristics of the gbap signal and uncovered the most potent quorum sensing activator in e. faecalis known to date. | 2017 | 28590764 |
| 2,6-difluorobenzamide inhibitors of bacterial cell division protein ftsz: design, synthesis, and structure-activity relationships. | a wide variety of drug-resistant microorganisms are continuously emerging, restricting the therapeutic options for common bacterial infections. antimicrobial agents that were originally potent are now no longer helpful, due to their weak or null activity toward these antibiotic-resistant bacteria. in addition, none of the recently approved antibiotics affect innovative targets, resulting in a need for novel drugs with innovative antibacterial mechanisms of action. the essential cell division pro ... | 2017 | 28586174 |
| susceptibility pattern of enterococci at tertiary care hospital. | the study was aimed to characterize enterococci from various clinical specimens, to determine the antimicrobial susceptibility pattern, and to explore the association between virulence factors and antimicrobial resistance. a total of 283 clinical enterococcal isolates were speciated and subjected to antimicrobial susceptibility testing. virulence factors (hemolysin, gelatinase, and biofilm production) were detected phenotypically. of the 283 enterococci isolated, 12 species were identified; pred ... | 2017 | 28584459 |
| can the enterococcus faecalis identified in the root canals of primary teeth be a cause of failure of endodontic treatment? | this study investigated the presence of enterococcus faecalis in primary teeth with primary root canal infections and related to the possible failure of pulpectomy outcome after 36 months. | 2017 | 28580816 |
| the role of reactive oxygen species in the antimicrobial activity of pyochelin. | the increase in prevalence of antimicrobial-resistant bacteria (arb) is currently a serious threat, thus there is a need for new antimicrobial compounds to combat infections caused by these arb. an antimicrobial-producing bacterium, burkholderia paludis was recently isolated and was able to produce a type of siderophore with antimicrobial properties, later identified as pyochelin. the chelating ability of pyochelin has been well-characterized but not for its antimicrobial characteristics. it was ... | 2017 | 28580180 |
| antibacterial properties of chitosan nanoparticles and propolis associated with calcium hydroxide against single- and multispecies biofilms: an in vitro and in situ study. | the aim of this study was to evaluate the efficacy of chitosan nanoparticles (cnps) and ethanolic propolis extract (epe) incorporated into a calcium hydroxide paste (ca[oh]2) to kill bacterial biofilms. | 2017 | 28578886 |
| genotyping, antimicrobial resistance and virulence factor gene profiles of vancomycin resistance enterococcus faecalis isolated from blood culture. | enterococcus spp. is the common of intestinal micro flora in humans but nowadays this gram-positive bacterium causes the variety of nosocomial infections. resistance to antibiotic and also, presence of different virulence genes in the enterococcus spp. can change it to problematic microorganisms in the health care centers. the aim of this study was determined the genotyping, antimicrobial resistance and virulence factor gene profiles of vancomycin resistance enterococcus faecalis isolated from b ... | 2017 | 28578090 |
| analysis of the incidence and risk factors of male urinary tract infection following urodynamic study. | the purpose of this study was to investigate the incidence and risk factors of male urinary tract infection (uti) after urodynamic study (uds). a total of 854 consecutive male patients, who underwent uds at peking union medical college hospital from january 2010 to march 2016, were recruited in this study. two to four weeks before the examination, urinalysis with bacterial culture was performed. patients with negative results were selected for uds. immediately before the examination, urinalysis ... | 2017 | 28577157 |
| bactericidal activity and synergy studies of peptide ap-cect7121 against multi-resistant bacteria isolated from human and animal soft tissue infections. | ap-cect7121 is an antimicrobial peptide, produced by enterococcus faecalis cect7121, with bactericidal activity against gram-positive bacteria. the aim of this study was to evaluate the bactericidal activity of ap-cect7121, alone and with gentamicin, against multi-resistant bacteria isolated from human and animals with soft tissue infections. during the period 2014-2015, bacterial strains producing human and animal soft tissue infections were studied. samples from patients attended at a general ... | 2017 | 28573492 |
| detection of the optra gene in a clinical st16 enterococcus faecalis isolate in denmark. | 2017 | 28572038 | |
| phytochemical characterisation and bioprospection for antibacterial and antioxidant activities of lippia alba brown ex britton & wilson (verbenaceae). | ethanol extract and fractions obtained from fresh and dry aerial parts of lippia alba were examined in order to determine their phytochemical composition, antioxidant capacity and antibacterial activities. the ethanol extracts and fractions exhibited an antioxidant effect by the dpph assay, especially samples of fresh plant. hplc analysis of the ethyl acetate fractions identified the presence of phenolic acids and flavonoids. the ethanol extract and fractions showed activity against reference an ... | 2017 | 28571495 |
| comparative evaluation of different modes of laser assisted endodontics in primary teeth: an in vitro study. | the presence of bacteria in root canals has been considered to be responsible for endodontic treatment failure, even in case of primary teeth. the use of lasers can be a valuable addition in removing bacterial load in areas where traditional methods may fail to succeed. methods like direct laser irradiation of canals, laser activated irrigation (lai) with sodium hypochlorite (naocl) and photodynamic therapy (pdt) seem to be a promising alternative for disinfection. | 2017 | 28571279 |
| effects of diode laser, gaseous ozone, and medical dressings on enterococcus faecalis biofilms in the root canal ex vivo. | the objective was to compare the antibacterial effects of adjunctive disinfection using diode laser and gaseous ozone compared to the medical dressings calcium hydroxide (ca(oh)2) and chlorhexidine gel (chx-gel) on enterococcus faecalis biofilms in human root canals ex vivo. root canals of 180 human extracted teeth were infected by e. faecalis and divided into 3 main groups (g): g1, control; g2, instrumentation and irrigation using 0.9% nacl; g3, instrumentation and irrigation using 1% naocl. in ... | 2017 | 28567421 |
| virulence factors associated with enterococcus faecalis infective endocarditis: a mini review. | the enterococci are accountable for up to 20% of all cases of infective endocarditis, with enterococcus faecalis being the primary causative isolate. infective endocarditis is a life-threatening infection of the endocardium that results in the formation of vegetations. based on a literature review, this paper provides an overview of the virulence factors associated with e. faecalis infective endocarditis. furthermore, it reports the effects of active or passive immunization against some of these ... | 2017 | 28567146 |
| human bile reduces antimicrobial activity of selected antibiotics against enterococcus faecalis and escherichia coli in vitro. | it has been known from previous studies that body fluids, such as cerebrospinal fluid, lung surfactant, and urine, have a strong impact on the bacterial killing of many anti-infective agents. however, the influence of human bile on the antimicrobial activity of antibiotics is widely unknown. human bile was obtained and pooled from 11 patients undergoing cholecystectomy. after sterilization of the bile fluid by gamma irradiation, its effect on bacterial killing was investigated for linezolid (lzd ... | 2017 | 28559254 |
| collaborative research on puerperal infections in bangladesh. | bangladesh is considered as a high-risk country for emerging infectious diseases because of its high population density, poverty, and unhygienic conditions. although control efforts have primarily been focused on major infectious diseases such as diarrheal diseases, tuberculosis, malaria, and hiv infection, the prevalence and impact of many local or minor infectious diseases are still unclarified in this country. in this review, we present our recent experience and outcomes of collaborative rese ... | 2017 | 28552890 |
| structure and dimerization of ireb, a negative regulator of cephalosporin resistance in enterococcus faecalis. | enterococcus faecalis, a leading cause of hospital-acquired infections, exhibits intrinsic resistance to most cephalosporins, which are antibiotics in the beta-lactam family that target cell-wall biosynthesis. a comprehensive understanding of the underlying genetic and biochemical mechanisms of cephalosporin resistance in e. faecalis is lacking. we previously determined that a transmembrane serine/threonine kinase (irek) and its cognate phosphatase (irep) reciprocally regulate cephalosporin resi ... | 2017 | 28551334 |
| a novel polytetrafluoroethylene-channel model, which simulates low levels of culturable bacteria in buildup biofilm after repeated endoscope reprocessing. | clinical studies have shown variable culture results from flexible endoscope channels possibly because of low levels of bacteria that are difficult to extract. the aim of this study was to develop a simulated-use buildup biofilm (bbf) model that mimics low levels of viable bacteria after repeated rounds of aldehyde fixation and accumulation. | 2017 | 28551023 |
| comparative evaluation of micron- and nano-sized intracanal medicaments on penetration and fracture resistance of root dentin - an in vitro study. | intracanal medicaments play a vital role in disinfection of root canal system. the aim of this study was to evaluate intratubular penetration and fracture resistance of roots filled with micron- and nano-sized intracanal medicaments: calcium hydroxide (ch), nanocalcium hydroxide (nch), chitosan (cs) and nanochitosan (ncs). their antibacterial effect on e. faecalis was tested using agar diffusion method. nch and ncs were prepared by precipitation method and ionic crosslinking respectively. nch an ... | 2017 | 28545965 |
| identification of enterococcus faecalis enzymes with azoreductases and/or nitroreductase activity. | nitroreductases, nad(p)h dependent flavoenzymes, are found in most of bacterial species. even if enterococcus faecalis strains seems to present such activity because of their sensitivity to nitrofurans, no enzyme has been described. nitroreductases were separated of others reductases due to their capacity to reduce nitro compounds. they are further classified based on their preference in cofactor: nadh and/or nadph. however, recently, azoreductases have been studied for their strong activity on ... | 2017 | 28545445 |
| influence of different wild-garlic (allium ursinum) extracts on the gastrointestinal system: spasmolytic, antimicrobial and antioxidant properties. | as there are no previous studies of the european wild-garlic (allium ursinum) effects on the gastrointestinal system, despite its traditional applications in gastrointestinal disorders' treatment and regular use in the human diet, we have quantified and compared spasmolytic, antimicrobial and antioxidant activities of its different leaf extracts. | 2017 | 28543032 |
| antimicrobial resistance status of enterococcus from australian cattle populations at slaughter. | antimicrobial agents are used in cattle production systems for the prevention and control of bacterial associated diseases. a consequence of their use is the potential development of antimicrobial resistance (amr). enterococcus faecium and enterococcus faecalis that are resistant to antimicrobials are of increased concern to public health officials throughout the world as they may compromise the ability of various treatment regimens to control disease and infection in human medicine. australia i ... | 2017 | 28542602 |
| emergence of vana enterococcus faecium in denmark, 2005-15. | to describe the changing epidemiology of vancomycin-resistant enterococcus faecium and enterococcus faecalis in clinical samples in denmark 2005-15 according to species and van type, and, furthermore, to investigate the genetic relatedness of the clinical e. faecium isolates from 2015. | 2017 | 28541565 |
| the bacteriophage ef-p29 efficiently protects against lethal vancomycin-resistant enterococcus faecalis and alleviates gut microbiota imbalance in a murine bacteremia model. | enterococcus faecalis is becoming an increasingly important opportunistic pathogen worldwide, especially because it can cause life-threatening nosocomial infections. treating e. faecalis infections has become increasingly difficult because of the prevalence of multidrug-resistant e. faecalis strains. because bacteriophages show specificity for their bacterial hosts, there has been a growth in interest in using phage therapies to combat the rising incidence of multidrug-resistant bacterial infect ... | 2017 | 28536572 |
| effect of gnp functionalisation and multiple n-methylation of β-amyloid residue (32-37) on gram-positive bacterium. | in the previous report, the authors showed the gold nanoparticle (gnp) functionalised multiple n-methylated fragments of the residue (32-37) of beta (β)-amyloid protein (1-42), cggiglmvg and cgggggiglmvg toward disruption of β-amyloid (1-42), the predominant component of senile plaques. herein the in vitro antimicrobial activities of both normal and multiple n-methylated sequences of cggiglmvg and cgggggiglmvg were screened and it was found that all the eight sequences including four (non-functi ... | 2017 | 28530185 |
| occurrence of enterococcus faecalis and enterococcus faecium in various clinical infections: detection of their drug resistance and virulence determinants. | the aim of this study was to characterize virulence determinants and antibiotic resistance profiles in enterococci obtained from various clinical sources in the northwest of iran. a total of 160 enterococcal clinical isolates from various wards of university teaching hospitals were collected and specified by biochemical test, from september 2014 to july 2015. identification of enterococci was confirmed by multiplex pcr in the genus and species level. antibiotic resistance properties and virulenc ... | 2017 | 28525287 |
| mechanisms of peptide sex pheromone regulation of conjugation in enterococcus faecalis. | in many gram positive bacteria, horizontal transfer and virulence are regulated by peptide-mediated cell-cell signaling. the heptapeptide ccf10 (c) activates conjugative transfer of the enterococcus faecalis plasmid pcf10, whereas the icf10 (i) peptide inhibits transfer. both peptides bind to the same domain of the master transcription regulator prgx, a repressor of transcription of the prgq operon encoding conjugation genes. we show that repression of prgq by prgx tetramers requires formation o ... | 2017 | 28523739 |
| water and air ozone treatment as an alternative sanitizing technology. | we investigated the effectiveness of ozone (aqueous and gaseous) treatment as an alternative sanitizing technology to common conventional disinfectants in reducing the microbial contamination of both water and air. | 2017 | 28515631 |
| comparison of simplistic biofilm models for evaluating irrigating solutions. | many innovations have recently been implemented in the field of endodontics, often based on a variety of in-vitro/ex-vivo test setups. it was the goal of this research to compare different biofilm models for evaluating the effectiveness of rinsing solutions. | 2017 | 28512652 |
| comparative antimicrobial efficacy of eucalyptus galbie and myrtus communis l. extracts, chlorhexidine and sodium hypochlorite against enterococcus faecalis. | the aim of this study was to evaluate the antimicrobial effect of eucalyptusgalbie and myrtus communis l. methanolic extracts, chlorhexidine (chx) and sodium hypochlorite (naocl) on enterococcus faecalis (e. faecalis) as the predominant species isolated from infected root canals. | 2017 | 28512487 |
| comparative evaluation of three herbal solutions on the disinfection of guttapercha cones: an in vitro study. | guttapercha (gp) cones are usually supplied in aseptic, sealed packages from the manufacturers, but once exposed to the dental office environment or even by handling; they can be contaminated by a number of microorganisms. supplementary decontamination of gp cones is critical. as they cannot be sterilized by moist or dry heat, cold sterilization, using disinfectants should be used. | 2017 | 28511502 |
| rapid identification of vancomycin resistant enterococcus faecalis clinical isolates using a sugar fermentation method. | vancomycin resistant enterococci (vre) can be found all over the world. thus, rapid detection of the isolates could be of high importance in the treatment or prevention of the associated disease. | 2017 | 28511382 |
| development of a panel of recombinase polymerase amplification assays for detection of common bacterial urinary tract infection pathogens. | to develop and evaluate the performance of a panel of isothermal real-time recombinase polymerase amplification (rpa) assays for detection of common bacterial urinary tract infection (uti) pathogens. | 2017 | 28510991 |
| antimicrobial, anticancer, and antioxidant compounds from premna resinosa growing in saudi arabia. | premna resinosa (hochst.) schauer (lamiaceae) is used in many places to treat bronchitis, respiratory illness and convulsions of the rib cage. | 2017 | 28508699 |
| the effect of sodium hypochlorite concentration and irrigation needle extension on biofilm removal from a simulated root canal model. | to investigate the effect of sodium hypochlorite concentration and needle extension on removal of enterococcus faecalis biofilm, sixty root canal models were 3d printed. biofilms were grown on the apical 3 mm of the canal for 10 days. irrigation for 60s with 9 ml of either 5.25% or 2.5% naocl or water was performed using a needle inserted either 3 or 2 mm from the canal terminus and imaged using fluorescence microscopy and residual biofilm percentages were calculated using imaging software. the ... | 2017 | 28508523 |
| empyema thoracis caused by an optra-positive and linezolid-intermediate enterococcus faecalis strain. | 2017 | 28506787 | |
| chemical composition, antibacterial, schistosomicidal, and cytotoxic activities of the essential oil of dysphania ambrosioides (l.) mosyakin & clemants (chenopodiaceae). | we have investigated the chemical composition and the antibacterial activity of the essential oil of dysphania ambrosioides (l.) mosyakin & clemants (chenopodiaceae) (da-eo) against a representative panel of cariogenic bacteria. we have also assessed the in vitro schistosomicidal effects of da-eo on schistosoma mansoni and its cytotoxicity to gm07492-a cells in vitro. gas chromatography (gc) and gas chromatography-mass spectrometry (gc/ms) revealed that the monoterpenes cis-piperitone oxide (35. ... | 2017 | 28504841 |
| metabolic impact of nutrient starvation in mevalonate-producing escherichia coli. | the aim of this work was to enhance mevalonate yield from glucose in escherichia coli by essential nutrient starvations and to reveal these effects on the central carbon metabolism. stationary phase culture without essential nutrients such as nitrogen, sulfur, and magnesium was evaluated using an engineered e. coli introducing mvae and mvas genes from enterococcus faecalis. sulfur starvation resulted in the highest mevalonate yield of 0.61c-molc-mol(-1) from glucose. the metabolic impacts of nut ... | 2017 | 28501379 |
| occurrence of ampicillin-resistant enterococcus faecium carrying esp gene in pet animals: an upcoming threat for pet lovers. | this study was carried out to investigate oral colonisation by enterococcus faecalis and enterococcus faecium in pet dogs and cats, with special reference to antibiotic resistance. | 2017 | 28499907 |
| an iterative glycosyltransferase ents catalyzes transfer and extension of o- and s-linked monosaccharide in enterocin 96. | glycosyltransferases are essential tools for in vitro-glycoengineering. bacteria harbor an unexplored variety of protein glycosyltransferases. here, we describe a peptide glycosyltransferase (ents) encoded by orf0417 of enterococcus faecalis tx0104. ents di-glycosylates linear peptide of enterocin 96- a known antibacterial, in vitro. it is capable of transferring as well as extending the glycan onto the peptide in an iterative sequential dissociative manner. it can catalyze multiple linkages: gl ... | 2017 | 28498962 |
| etiological and resistance profile of bacteria involved in urinary tract infections in young children. | background. the objective of this study was to identify the bacteria most frequently responsible for urinary tract infection (uti) in the population of under-2-year-olds in our geographic area and to evaluate the activity of antibiotics widely used for uti treatment during a 4-year study period. materials and methods. a retrospective analysis was conducted of data on the identification and susceptibility of microorganisms isolated in urine samples from children under 2 years of age. results. a t ... | 2017 | 28497052 |
| antimicrobial resistance patterns in women with positive urine culture: does menopausal status make a significant difference? | aim. urinary tract infection (uti) is considered one of the most common bacterial infections in women. the aim of this study was to investigate the types of uropathogens present, as well as the degree of antimicrobial drug resistance seen among premenopausal (n = 2748) and postmenopausal (n = 1705) women with uncomplicated uti. methods. urinary samples (n = 4453) collected from women with uti were analyzed in terms of uropathogens present. these were considered as positive if bacterial growth wa ... | 2017 | 28497048 |
| evaluation of the synergistic effect of tomatidine with several antibiotics against standard and clinical isolates of staphylococcus aureus, enterococcus faecalis, pseudomonas aeruginosa and escherichia coli. | antibiotic resistance is an important problem in antibiotic treatment of infections, particularly in hospitals. tomatidine is a plant secondary metabolite with antimicrobial and antifungal effects. this study examined the possible synergistic effect tomatidine with several antibiotics against standard and clinical strains of staphylococcus aureus, enterococcus faecalis, pseudomonas aeruginosa and escherichia coli. after determining the minimum inhibitory concentrations (mics) of antibiotics and ... | 2017 | 28496482 |
| chemical composition, antibacterial and antifungal activities of flowerhead and root essential oils of santolina chamaecyparissus l., growing wild in tunisia. | the antimicrobial properties of essential oil from various santolina species have not been investigated enough in the previous studies dealing with the biological activities of medicinal plants. in tunisia, santolina chamaecyparissus l. (asteraceae) is the only santolina species recorded and is used as vermifuge and emmenagogue. the chemical composition, antibacterial and antifungal properties of essential oils from the flowerheads and roots of spontaneous s. chamaecyparissus growing in tunisia ... | 2017 | 28490960 |
| the most frequent isolates from outpatients with urinary tract infection. | the main objective of the study was to identify the most frequent isolates from female outpatients with urinary tract infection. | 2017 | 28484348 |
| five-year summary of in vitro activity and resistance mechanisms of linezolid against clinically important gram-positive cocci in the united states from the leader surveillance program (2011 to 2015). | this report describes linezolid susceptibility testing results for 6,741 gram-positive pathogens from 60 u.s. sites collected during 2015 for the leader program. in addition, the report summarizes linezolid in vitro activity, resistance mechanisms, and molecular typing obtained for 2011 to 2015. during 2015, linezolid showed potent activity in testing against staphylococcus aureus, inhibiting >99.9% of 3,031 isolates at ≤2 µg/ml. similarly, linezolid showed coverage against 99.2% of coagulase-ne ... | 2017 | 28483950 |
| association of metal homeostasis and (p)ppgpp regulation in the pathophysiology of enterococcus faecalis. | in enterococcus faecalis, the regulatory nucleotides pppgpp and ppgpp, collectively, (p)ppgpp, are required for growth in blood, survival within macrophages, and virulence. however, a clear understanding of how (p)ppgpp promotes virulence in e. faecalis and other bacterial pathogens is still lacking. in the host, the essential transition metals iron (fe) and manganese (mn) are not readily available to invading pathogens because of a host-driven process called nutritional immunity. considering it ... | 2017 | 28483855 |
| cytoprotective effect of the enzyme-mediated polygallic acid on fibroblast cells under exposure of uv-irradiation. | the poly(gallic acid), produced by laccase-mediated oxidation of gallic acid in aqueous media (ph5.5) to attain a novel material with well-defined molecular structure and high water solubility (500mg/ml at 25°c), has been investigated to understand its potential biological activities. in this regard, a biomedical approach based on cytoprotective effect on human fibroblast cells exposed to uv-irradiation in the presence of the polymer has been demonstrated. the results also shows that 200μg/ml of ... | 2017 | 28482545 |
| use of photo-fenton's reaction by 400-nm led light for endodontic disinfection: a preliminary in vitro study on enterococcus faecalis. | one of the biggest challenges in endodontics is the complete disinfection of root canals. in addition to mechanical preparation, the technique traditionally also involves channel disinfection with other agents such as sodium hypochlorite, hydrogen peroxide, chlorhexidine, or a combination of these. some bacterial species are particularly resistant to eradication. using enterococcus faecalis in this preliminary study, we tested the bactericidal effectiveness of the fenton reaction and the photo-f ... | 2017 | 28482224 |
| daptomycin: new insights into an antibiotic of last resort. | 2017 | 28481142 | |
| studies on chemical composition and antimicrobial activities of bioactive molecules from date palm (phoenixdactylifera l.) pollens and seeds. | natural resources have been the crucial origin of chemical elements. they have been used in many traditions as alternative medicines. the chemical profiling of some plant extracts and essential oils related to different plants were followed to unveil their most active components. in this paper, phoenix dactilyfera l was selected as a host plant to investigate the composition of different organs with different cultivars. | 2017 | 28480436 |
| does the removal of cementum facilitate bacterial penetration into dentinal tubules in vitro? | the importance of an intact layer of cementum on the root surface in preventing bacterial penetration into radicular dentin has not been sufficiently investigated. the aim of this in vitro study was to determine the effect of the absence of cementum from the root surface and the length of the infection period (2 or 4 weeks) on the maximum depth of bacterial penetration and the percentage of sectors lined with bacteria. | 2017 | 28476468 |
| urinary tract infections in postmenopausal women with type 2 diabetes: clinical correlates and quinolone susceptibility. | introduction diabetes mellitus and the postmenopausal period are associated with increased risk of urinary tract infections (utis) in women. however, data on utis in postmenopausal diabetic women are scarce. objectives the aim of this study was to determine the prevalence of utis in postmenopausal women with type 2 diabetes mellitus, identify the potential risk factors, describe the causative pathogens, and assess their susceptibility to quinolones. patients and methods patients were interviewe ... | 2017 | 28475170 |
| a short artificial antimicrobial peptide shows potential to prevent or treat bone infections. | infection of bone is a severe complication due to the variety of bacteria causing it, their resistance against classical antibiotics, the formation of a biofilm and the difficulty to eradicate it. antimicrobial peptides (amps) are naturally occurring peptides and promising candidates for treatment of joint infections. this study aimed to analyze the effect of short artificial peptides derived from an optimized library regarding (1) antimicrobial effect on different bacterial species, (2) efficac ... | 2017 | 28473710 |
| chicken embryo lethality assay for determining the lethal dose and virulence of enterococcus faecalis. | enterococcus faecalis is the major pathogen found in field cases of amyloid arthropathy in chickens. given the need for a better understanding of the virulence mechanisms of the causative strains, the embryo lethality assay (ela) is proposed in the present study as a model to evaluate the virulence of e. faecalis strains, specifically the pathogenic avian strain k923/96, which was previously related with amyloid arthropathy. hence, 0.2 ml of five doses of the cited strain (from 2.5 to 2500 colon ... | 2017 | 28470098 |
| evaluation of antibacterial efficacy of photodynamic therapy vs. 2.5% naocl against e. faecalis-infected root canals using real-time pcr technique. | bacteria like e. faecalis can produce intra- and extra-radicular biofilms. theoretically, the adjustable penetration ability of lasers enables better access to root canal system. therefore the aim of the present study was to compare the ability of photoactivated laser and 2.5% naocl irrigation solution to eliminate e. faecalis from the root canals by real-time pcr technique. | 2017 | 28469819 |
| role of surface energy and nano-roughness in the removal efficiency of bacterial contamination by nonwoven wipes from frequently touched surfaces. | healthcare associated infections (hcais) are responsible for substantial patient morbidity, mortality and economic cost. infection control strategies for reducing rates of transmission include the use of nonwoven wipes to remove pathogenic bacteria from frequently touched surfaces. wiping is a dynamic process that involves physicochemical mechanisms to detach and transfer bacteria to fibre surfaces within the wipe. the purpose of this study was to determine the extent to which systematic changes ... | 2017 | 28469734 |
| molecular mechanism of quorum-sensing in enterococcus faecalis: its role in virulence and therapeutic approaches. | quorum-sensing systems control major virulence determinants in enterococcusfaecalis, which causes nosocomial infections. the e. faecalis quorum-sensing systems include several virulence factors that are regulated by the cytolysin operon, which encodes the cytolysin toxin. in addition, the e. faecalis fsr regulator system controls the expression of gelatinase, serine protease, and enterocin o16. the cytolysin and fsr virulence factor systems are linked to enterococcal diseases that affect the hea ... | 2017 | 28467378 |
| total staphylococci as performance surrogate for greywater treatment. | faecal indicator bacteria (fib) are commonly used as water quality indicators; implying faecal contamination and therefore the potential presence of pathogenic enteric bacteria, viruses, and protozoa. hence in wastewater treatment, the most commonly used treatment process measures (surrogates) are total coliforms, faecal coliforms, escherichia coli (e. coli), and enterococci. however, greywater potentially contains skin pathogens unrelated to faecal load, and e. coli and other fib may grow withi ... | 2017 | 28462431 |
| applications of copolymer for rapid identification of bacteria in blood culture broths using matrix-assisted laser desorption ionization time-of-flight mass spectrometry. | matrix-assisted laser desorption ionization time-of-flight mass spectrometry (maldi-tof ms) can be used to identify pathogens in blood culture samples. however, sample pretreatment is needed for direct identification of microbes in blood culture bottles. conventional protocols are complex and time-consuming. therefore, in this study, we developed a method for collecting bacteria using polyallylamine-polystyrene copolymer for application in wastewater treatment technology. using representative ba ... | 2017 | 28461023 |
| bacterial diversity of the gastric content of preterm infants during their first month of life at the hospital. | studies focused on the stomach microbiota are relatively scarce, and most of them are focused on the adult population. the aim of this work is to describe the bacterial communities inhabiting the gastric content (gc) of preterm neonates. for that purpose, gc samples were collected weekly from a total of 13 preterm neonates during their first month of life within their hospital stay. samples were analyzed by using both culture-dependent and -independent techniques. the former allowed the isolatio ... | 2017 | 28459051 |
| transcriptomics analysis reveals putative genes involved in biofilm formation and biofilm-associated drug resistance of enterococcus faecalis. | enterococcus faecalis is a gram-positive bacterium associated with endodontic infections and is capable of forming biofilms that can confer drug resistance to the bacterium, resulting in treatment failure. current knowledge on e. faecalis drug resistance is of a limited and conflicting nature. the present study examined the genetic basis of e. faecalis biofilm formation and drug resistance using a rna sequencing (rna-seq)-based transcriptome approach. | 2017 | 28457636 |
| high-purity nisin alone or in combination with sodium hypochlorite is effective against planktonic and biofilm populations of enterococcus faecalis. | nisin, a broad-spectrum bacteriocin, has recently been highlighted for its biomedical applications. to date, no studies have examined the antimicrobial and antibiofilm properties of high-purity (>95%) nisin (nisin zp) on enterococcus faecalis and biofilms formed by this species. we hypothesize that nisin can inhibit e. faecalis and reduce biofilm biomass, and combinations of nisin and sodium hypochlorite (naocl) will enhance the antibiofilm properties against e. faecalis biofilms. | 2017 | 28457635 |
| enzymes required for maltodextrin catabolism in enterococcus faecalis exhibit novel activities. | maltose and maltodextrins are formed during the degradation of starch or glycogen. maltodextrins are composed of a mixture of maltooligosaccharides formed by α-1,4- but also some α-1,6-linked glucosyl residues. the α-1,6-linked glucosyl residues are derived from branching points in the polysaccharides. in enterococcus faecalis, maltotriose is mainly transported and phosphorylated by a phosphoenolpyruvate:carbohydrate phosphotransferase system. the formed maltotriose-6″-phosphate is intracellular ... | 2017 | 28455338 |
| response surface method optimization of a novel hypericin formulation in p123 micelles for colorectal cancer and antimicrobial photodynamic therapy. | the photodynamic properties of hypericin (hyp) may be used as an alternative treatment for malignancies of the lower gastrointestinal tract and for the prevention of surgical-site infection; however, its use in photodynamic therapy has been limited because of its poor hydrosolubility. therefore, in order to improve its water solubility and its photodynamic effect, hyp was encapsulated in pluronic p123 (p123) and the photodynamic effects against intestinal and epidermal bacteria and against two l ... | 2017 | 28454049 |