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outcome of treated and untreated asymptomatic bacteriuria in renal transplant recipients.background: no guidelines exist concerning treatment of asymptomatic bacteriuria in renal transplant recipients (rtr). because of scarce clinical symptoms and fear of complications, such episodes are frequently treated based on subjective criteria without clear clinical benefit, with the risk of selecting resistant pathogens. methods: we retrospectively analysed the outcome of 334 asymptomatic escherichia coli (e. coli) and enterococcus faecalis (e. faecalis) bacteriuria that occurred in 77 rtr ...201121592976
sensitivity to disinfection of bacterial indicator organisms for monitoring the salmonella enteritidis status of layer farms after cleaning and disinfection.the present study evaluated escherichia coli, enterococcus faecalis, and enterococcus hirae as potential indicator organisms for the possible salmonella enteritidis (se) presence in layer farms after cleaning and disinfection by comparing their susceptibility to disinfection. a quantitative suspension disinfection test according to european standard en1656 was performed using disinfection products cid20 and virocid (both from cid lines, ieper, belgium). in a preliminary test, the sensitivity to ...201121597057
structure of qnrb1, a plasmid-mediated fluoroquinolone resistance factor.qnrb1 is a plasmid-encoded pentapeptide repeat protein (prp) that confers a moderate degree of resistance to fluoroquinolones. its gene was cloned into an expression vector with an n-terminal polyhistidine tag, and the protein was purified by nickel affinity chromatography. the structure of qnrb1 was determined by a combination of trypsinolysis, surface mutagenesis, and single anomalous dispersion phasing. qnrb1 folds as a right-handed quadrilateral β-helix with a highly asymmetric dimeric struc ...201121597116
microbicidal activities of low frequency atmospheric pressure plasma jets on oral pathogens.research using low frequency atmospheric pressure plasma jets (lf jet) is becoming increasingly more common. we carried out experiments to evaluate the sterilizing effects of this technology on oral pathogenic microorganisms (s.mutans, c.albicans and e. faecalis) and to determine its potential for clinical application. we performed the direct exposure test on a solid surface, indirect exposure test on a liquid phase, and ros (reactive oxygen species) inhibitory test. the results showed the lf je ...201121597211
pharmacokinetics of free ertapenem in critically ill septic patients: intermittent versus continuous infusion.background: the aim of this paper was to compare the pharmacokinetic and pharmacodynamic (pk/pd) parameters of continuous (ci) and intermittent infusion (iti) of ertapenem into critically ill patients with severe abdominal infections. methods: twenty septic patients hospitalized in a university hospital intensive care unit were enrolled in the study. half of the patients received ertapenem as an iti 1 g bolus once daily, and the other half of the patients received the same dose via ci over 24 h ...201121597443
secondary mania associated with enterococcal meningitis in an elderly patient.primary mania is associated with bipolar disorder, whereas secondary mania may result from many etiologies, including metabolic, pharmacological and neurological causes. strong evidence has shown that, in older adults, new onset mania is more likely associated with an underlying cause, suggesting that late-onset mania is often secondary [bipolar disord. 2004;6(5):343-67]. cryptococcal meningitis has been reported to induce secondary mania [gen hosp psychiatry. 2005;27(4):301-3]. here, we present ...201121601728
the significance of a glutamate-139 residue of v-type na+-atpase ntpk subunit in its catalytic turnover linked with salt tolerance of enterococcus hirae.an ntpk subunit glu139asp (ke139d) mutant of enterococcus hirae v-atpase lost tolerance to sodium but not to lithium at ph 10. purified ke139d v-atpase retained relatively high specific activity and affinity for lithium ion compared to sodium ion. the ke139 residue of v-atpase is indispensable for its enzymatic activity linked with salt tolerance of enterococci.201121602356
impact of medicated feed on the development of antimicrobial resistant bacteria in integrated pig-fish farms in vietnam.integrated livestock-fish aquaculture utilizes animal excreta, urine, and feed leftovers as pond fertilizers to enhance growth of plankton and other microorganisms eaten by the fish. however, antimicrobial resistant bacteria may be transferred and develop in the pond due to selective pressure from antimicrobials present in animal feed, urine, and feces. in an experimental pig-fish farm located in peri-urban hanoi, vietnam, nine piglets were provided feed containing 5 μg/kg pig weight/day of tetr ...201121602395
vancomycin-resistant enterococcus faecium bacteremia successfully treated with high-dose ampicillin-sulbactam in a pediatric patient after hematopoietic stem cell transplantation. 201121602724
a comprehensive analysis of gene expression changes provoked by bacterial and fungal infection in c. elegans.while caenorhabditis elegans specifically responds to infection by the up-regulation of certain genes, distinct pathogens trigger the expression of a common set of genes. we applied new methods to conduct a comprehensive and comparative study of the transcriptional response of c. elegans to bacterial and fungal infection. using tiling arrays and/or rna-sequencing, we have characterized the genome-wide transcriptional changes that underlie the host's response to infection by three bacterial (serr ...201121602919
assessing environmental impacts of treated wastewater through monitoring of fecal indicator bacteria and salinity in irrigated soils.to assess the potential for treated wastewater irrigation to impact levels of fecal indicator bacteria (fib) and salinity in irrigated soils, levels of escherichia coli, enterococcus, and environmental covariates were measured in a treated wastewater holding pond (irrigation source water), water leaving the irrigation system, and in irrigated soils over 2 years in a municipal parkland in arizona. higher e. coli levels were measured in the pond in winter (56 cfu 100 ml(-1)) than in summer (17 cfu ...201121603923
screening of australian plants for antimicrobial activity against campylobacter jejuni.campylobacter jejuni is the most common cause of acute enteritis in humans, with symptoms such as diarrhoea, fever and abdominal cramps. in this study, 115 extracts from 109 australian plant species were investigated for their antimicrobial activities against two c. jejuni strains using an in vitro broth microdilution assay. among the plants tested, 107 (93%) extracts showed activity at a concentration between 32 and 1024 µg/ml against at least one c. jejuni strain. seventeen plant extracts were ...201121604309
convergent transcription confers a bistable switch in enterococcus faecalis conjugation.convergent gene pairs with head-to-head configurations are widespread in both eukaryotic and prokaryotic genomes and are speculated to be involved in gene regulation. here we present a unique mechanism of gene regulation due to convergent transcription from the antagonistic prgx/prgq operon in enterococcus faecalis controlling conjugative transfer of the antibiotic resistance plasmid pcf10 from donor cells to recipient cells. using mathematical modeling and experimentation, we demonstrate that c ...201121606359
the efficacy of a cervical barrier in preventing microleakage of enterococcus faecalis in endodontically treated teeth.the clinical failure of coronal restorations can compromise the healthy periapical status of endodontically treated teeth. the purpose of the present ex vivo study was to assess the effectiveness of the cervical barrier in preventing bacterial microleakage in the eventual loss of the coronal restoration. following removal of the smear layer and obturation to the cementoenamel junction using gutta-percha, 70 single-rooted mandibular premolars were randomly divided into five different groups: grou ...201121613029
recognition of pathogenic microbes by the drosophila phagocytic pattern recognition receptor eater.non-opsonic phagocytosis is a primordial form of pathogen recognition that is mediated by the direct interaction of phagocytic receptors with microbial surfaces. in the fruit fly drosophila melanogaster, the egf-like repeat containing scavenger receptor eater is expressed by phagocytes and is required to survive infections with gram-positive and gram-negative bacteria. however, the mechanisms by which this receptor recognizes different types of bacteria are poorly understood. to address this pro ...201121613218
essential oil composition and antibacteral activity of vismia baccifera fruits collected from mérida, venezuela.the essential oil from the fruits of vismia baccifera triana & planch. (gutttiferae), collected in june 2009, was analyzed by gc/ms. a yield of 0.6% oil was obtained by hydrodistillation. twenty-seven components were identified by comparison of their mass spectra with those in the wiley gc-ms library data base. the major components were trans-cadin-l1,4-diene (36.6%), cis-cadin-1,4-diene (18.8%) and beta-caryophyllene (11.9%). the essential oil showed a broad spectrum of antibacterial activity a ...201121615035
airway pathogens during the first year after lung transplantation: a single-center experience.after lung transplantation, a high level of immunosuppression is needed to prevent rejection. this demand renders recipients more sensitive to infections. as pulmonary infections are a major clinical problem during the first postoperative year after lung transplantation, preventive treatment and regular surveillance examinations are needed for immediate, adequate therapy. we describe the airway pathogens registered during the first posttransplantation year among our 12 lung transplant recipients ...201121620113
comparison of genotypic and phylogenetic relationships of environmental enterococcus isolates by box-pcr typing and 16s rrna gene sequencing.environmental enterococcus spp. were compared by box-pcr genotyping and 16s rrna sequencing to clarify the predictive relationship of box-pcr fingerprints to species designation. box-pcr and 16s rrna relationships agreed for 77% of strains. box-pcr provided superior intra-species discrimination, but incorrectly speciated some strains and divided some species into multiple groups.201121622792
role of the (mn)superoxide dismutase of enterococcus faecalis in the in vitro interaction with microglia.enterococcus faecalis is a significant human pathogen worldwide and is responsible for severe nosocomial and community-acquired infections. although enterococcal meningitis is rare, mortality is considerable, reaching 21 %. nevertheless, the pathogenetic mechanisms of this infection remain poorly understood, even though the ability of e. faecalis to avoid or survive phagocytic attack in vivo may be very important during the infection process. we previously showed that the manganese-cofactored su ...201121474536
linezolid-resistant enterococci in greece: epidemiological characteristics.background: 14 linezolid-resistant enterococci (6 enterococcus faecium and 8 enterococcus faecalis) collected during 2009 from patients hospitalized in intensive care units of different greek hospitals were investigated. methods: the mechanism of resistance to linezolid was determined by sequencing analysis of the domain v of 23s rdna, while the clonal relatedness was defined by pulsed-field gel electrophoresis and multilocus sequence typing. results: all linezolid-resistant enterococci carried ...201121474928
persistence and diversity of faecal coliform and enterococci populations in faecally polluted waters.aim:  to assess the persistence and diversity of faecal bacterial populations (faecal coliforms and enterococci) that have recently been included in microbial source tracking (mst) predictive models. methods and results:  the analysed bacterial populations included members of the enterococci group (ent) [enterococcus faecium (fm), enterococcus faecalis (fs) and enterococcus hirae (hir)] and the faecal coliform group (fc) [diverse escherichia coli phenotypes (ecp) and cellobiose-negative faecal c ...201121477066
quantifying the performance of pediococcus sp. (nrrl b-2354: enterococcus faecium) as a nonpathogenic surrogate for salmonella enteritidis pt30 during moist-air convection heating of almonds.pediococcus sp. nrrl b-2354 was investigated as a potential nonpathogenic surrogate for salmonella enterica serovar enteritidis phage type 30 (se pt30) on the surface of almonds subjected to moist-air heating. both microorganisms were subjected to various time, temperature, and humidity regimens on almonds processed in a computer-controlled, laboratory-scale, moist-air convection oven. overall, the mean log reductions for pediococcus sp. were 0.6 log and 1.4 log lower than those for se pt30 (p < ...201121477474
interception of teicoplanin oxidation intermediates yields new antimicrobial scaffolds.in the search for new efficacious antibiotics, biosynthetic engineering offers attractive opportunities to introduce minor alterations to antibiotic structures that may overcome resistance. dbv29, a flavin-containing oxidase, catalyzes the four-electron oxidation of a vancomycin-like glycopeptide to yield a40926. structural and biochemical examination of dbv29 now provides insights into residues that govern flavinylation and activity, protein conformation and reaction mechanism. in particular, t ...201121478878
enterococcus faecalis sufcdsub complements escherichia coli  sufabcdse.iron-sulfur [fe-s] clusters are inorganic prosthetic groups that play essential roles in all living organisms. iron and sulfur mobilization, formation of [fe-s] clusters, and delivery to its final protein targets involves a complex set of specific protein machinery. proteobacteria has three systems of [fe-s] biogenesis, designated nif, isc, and suf. in contrast, the firmicutes system is not well characterized and has only one system, formed mostly by suf homologs. the firmicutes phylum correspon ...201121480963
synthesis and in vitro antibacterial activity of 7-(3-alkoxyimino-4-amino-4-methylpiperidin-1-yl) fluoroquinolone derivatives.a series of novel 7-(3-alkoxyimino-4-amino-4-methylpiperidin-1-yl)fluoroquinolone derivatives were designed, synthesized and evaluated for their in vitro antibacterial activity and cytotoxicity. all of the target compounds have potent antibacterial activity against the tested gram-positive and gram-negative strains, and exhibit good potency in inhibiting the growth of staphylococcus aureus including mrsa, staphylococcus epidermidis including mrse and streptococcus pneumoniae (mics: 0.125-4μg/ml) ...201121481984
early life-threatening lactic acidosis following a single infusion of linezolid. 201121482079
identification of a new gene, vanv, in vanb operons of enterococcus faecalis.subinhibitory concentrations of vancomycin are known to induce a cell-wall stimulon in some gram-positive pathogens, but this has never been studied in the genus enterococcus. in this study, enterococcus faecalis v583 strain was submitted to a subinhibitory concentration of vancomycin. dna microarray technology was used to analyse the transcriptomic changes induced by this antibiotic. ef2292, annotated as a hypothetical protein in the e. faecalis v583 genome, was highly induced in response to va ...201121482081
impressive echocardiographic images of a mitral valve aneurysm. 201121482969
antimicrobial activities of indole alkaloids from tabernaemontana catharinensis.tabernaemontana catharinensis root bark ethanol extract, eb2 fraction and the mmv alkaloid (12-methoxy-4-methylvoachalotine) were evaluated for their antimicrobial activities. t. catharinensis ethanol extract was effective against both strains of the dermatophyte trichophyton rubrum at concentrations of 2.5 mg/ml (wild strain) and 1.25 mg/ml (mutant strain), while the eb2 fraction and mmv alkaloid showed a strong antifungal activity against wild and mutant strains with mic values of <0.02 and 0. ...201121425672
antimicrobial activity of the essential oil of greek endemic stachys spruneri and its main component, isoabienol.the essential oils of stachys spruneri boiss. (sample a and sample b) were analyzed by gc and gc-ms. (+)-isoabienol was the dominant component (49.5 and 48.2%, respectively of the total oils) among seventy-two identified constituents. isoabienol was separated, purified by preparative thin-layer chromatography, and further identified by means ofphysicochemical and spectrometric analysis. the microbial growth inhibitory properties of the essential oil and its main metabolite, the labdane diterpene ...201121425694
successful use of intravenous linezolid in capd patient with vancomycin-resistant enterococcal peritonitis. 201121427253
evaluating the importance of faecal sources in human-impacted waters.quantitative microbial risk assessment (qmra) was used to evaluate the relative contribution of faecal indicators and pathogens when a mixture of human sources impacts a recreational waterbody. the waterbody was assumed to be impacted with a mixture of secondary-treated disinfected municipal wastewater and untreated (or poorly treated) sewage, using norovirus as the reference pathogen and enterococci as the reference faecal indicator. the contribution made by each source to the total waterbody v ...201121429551
epidemiological features and resistance pattern in uropathogens isolated from chronic bacterial prostatitis.chronic bacterial prostatitis (cbp) is, usually, caused by uropathogens, especially gram-negative bacilli, although infection is sometimes due to gram-positive and atypical microorganisms. a recent increasing in prevalence of gram-positive strains has been reported. the aim of this study was to explore the epidemiological features and resistance rates in uropathogens isolated from cbp outpatients in last 10 years. all consecutive outpatients with demonstrated cbp attending a single sexually tran ...201121717331
health care-associated infections studies project: an american journal of infection control and national healthcare safety network data quality collaboration case study 6. 201121704430
chemical composition and antibacterial activity of the essential oil of lantana camara var. moritziana.the essential oil obtained from the leaves of lantana camara var. moritziana (otto & dietr.) l+¦pez-palacios collected at rubio, t+íchira state, venezuela, was obtained by hydrodistillation in a clevenger trap (0.1% yield). the oil was analyzed by gas chromatography-mass spectrometry (gc/ms) on hp gc-ms system, model 5973, identifying 33 compounds (97.1%) of which the major components were germacrene d (31.0%), followed by beta-caryophyllene (14.8%), a-phellandrene (6.7%), limonene (5.7%) and 1, ...201121834251
crystal structures of antibiotic-bound complexes of aminoglycoside 2''-phosphotransferase iva highlight the diversity in substrate binding modes among aminoglycoside kinases.aminoglycoside 2''-phosphotransferase iva [aph(2'')-iva] is a member of a family of bacterial enzymes responsible for medically relevant resistance to antibiotics. aph(2'')-iva confers high-level resistance against several clinically used aminoglycoside antibiotics in various pathogenic enterococcus species by phosphorylating the drug, thereby preventing it from binding to its ribosomal target and producing a bactericidal effect. we describe here three crystal structures of aph(2'')-iva, one in ...201121678960
risk factors for recurrence of clostridium difficile infection: effect of vancomycin-resistant enterococci colonization.recurrent clostridium difficile infection (cdi) is one of the most difficult problems in healthcare infection control. we evaluated the risk factors associated with recurrence in patients with cdi. a retrospective cohort study of 84 patients with cdi from december 2008 through october 2010 was performed at pusan national university yangsan hospital. recurrence occurred in 13.1% (11/84) of the cases and in-hospital mortality rate was 7.1% (6/84). stool colonization with vancomycin-resistant enter ...201121738336
evaluation of direct inoculation of the bd phoenix system from positive bactec blood cultures for both gram-positive cocci and gram-negative rods.rapid identification (id) and antibiotic susceptibility testing (ast) of the causative micro-organism of bloodstream infections result in earlier targeting of antibiotic therapy.in order to obtain results of id and ast up to 24 hours earlier, we evaluated the accuracy of direct inoculation of the phoenix system from positive blood cultures (bactec) by using serum separator tubes to harvest bacteria from positive blood cultures. results were compared to those of standard phoenix procedure. discre ...201121718482
[characteristics of microbiocenosis of gingival pocket in patients with chronic generalized periodontitis].as a result of examination of 56 patients with chronic generalized periodontitis (cgp) of a moderate severity and a severe form of the disease, microflora from gingival pocket in patients which severe form of cgp was established to have higher specific weight in index of dissemination of strains such as staphylococcus aureus, streptococcus pyogencs, enterococcus, corynebacterium spp., anaerobic bacteria. strains from patients with a severe form of cgp were characterized by a higher level of expr ...201121716238
molecular characterization of fecal microbiota in patients with viral diarrhea.the study provides molecular analyses of fecal microbiota of diarrhea patients infected with four different types of viruses. fecal specimens from 52 patients with viral diarrhea (13 each of adenovirus, norovirus, rotavirus, and astrovirus) and six healthy individuals were collected and etiological viral agent was confirmed by enzyme immunoassay and specific pcr. to assess the changes in microbial diversity in patients with viral diarrhea, dna from stool were extracted and characterized by pcr-d ...201121739252
acquired antibiotic resistance: are we born with it?the rapid emergence of antibiotic resistance (ar) is a major public health concern. recent findings on the prevalence of foodborne antibiotic resistant (art) commensal bacteria in ready-to-consume food products suggested that daily food consumption likely serves as a major avenue for disseminating art bacteria from the food chain to the human hosts. to properly assess the impact of various factors including the food chain on ar development in hosts, it is important to determine the baseline of a ...201121821748
validation of the z++rich burn-biofilm model.background: despite advances in the use of topical and parenteral antimicrobial therapy and the practice of early tangential burn-wound excision, bacterial infection remains a major problem in the management of burn victims today. the purpose of this study was to design and evaluate a polyspecies biofilm model with bacteria known to cause severe infections in burn patients. the model is simple to prepare, maintain and analyse, and allows for short-term exposure to antimicrobials. results: initia ...201121724333
[study of marine actinomycetes isolated from the central coast of peru and their antibacterial activity against methicillin-resistant staphylococcus aureus and vancomycin-resistant enterococcus faecalis].objectives. to determine the antimicrobial potential of marine actinomycetes against drug-resistant pathogens represented by strains of methicillin-resistant staphylococcus aureus (mrsa) and vancomycin-resistant enterococcus faecalis (vre). materials and methods. strains of actinomycetes (29) isolated from marine sediment were evaluated by their characteristics in two culture media and by testing their inhibitory capacity by in vitro antagonism against multi-drug resistant (mdr) pathogenic bacte ...201121845303
identification of the binding modes of n-phenylphthalimides inhibiting bacterial thymidylate synthase through x-ray crystallography screening.to identify specific bacterial thymidylate synthase (ts) inhibitors, we exploited phenolphthalein (pth), which inhibits both bacterial and human enzymes. the x-ray crystal structure of lactobacillus casei ts (lcts) that binds pth showed multiple binding modes of the inhibitor, which prevented a classical structure-based drug design approach. to overcome this issue, we synthesized two phthalimidic libraries that were tested against ts enzymes and then we performed x-ray crystallographic screening ...201121696158
disruption of the alssd operon of enterococcus faecalis impairs growth on pyruvate at low ph.diacetyl and acetoin are pyruvate-derived metabolites excreted by many micro-organisms, and are important in their physiology. although generation of these four-carbon (c4) compounds in enterococcus faecalis is a well-documented phenotype, little is known about the gene regulation of their biosynthetic pathway and the physiological role of the pathway in this bacterium. in this work, we identified the genes involved in c4 compound biosynthesis in ent. faecalis and report their transcriptional an ...201121719538
bacterial infection in association with snakebite: a-á10-year experience in a northern taiwan medical center.background: microbiological data of secondary wound infections following snakebites is rarely reported in taiwan. the objective of this study was to assess the secondary wound infection after venomous snakebites. methods: we conducted a 10-year retrospective survey on patients admitted for venomous snakebites and microbiological data of wound cultures at a medical center in northern taiwan. results: between april 2001 and april 2010, 231 patients who experienced snakebites were included. male pr ...201121700517
clonal lineages, antibiotic resistance and virulence factors in vancomycin-resistant enterococci isolated from fecal samples of red foxes (vulpes vulpes).fourteen vana-containing enterococcal isolates were detected in seven of 52 fecal samples (13.5%) from free-ranging red foxes in portugal. nine of the vana-containing isolates were enterococcus faecium and five were e. durans. both sequence types, st262 and st273, were identified among e. faecium isolates.201121719850
epidemiologic characteristics of resistant microorganisms present in reserves from an intensive care unit.in this study, significant contamination in the intensive care unit environment was observed (p < .004). sampling of inanimate surfaces and equipment found vancomycin-resistant enterococcus faecalis, ciprofloxacin-resistant staphylococcus aureus and s epidermidis, and multiresistant acinetobacter baumannii with similarities (60%-80%) to blood culture isolates.201121824685
sources and fate of salmonella and fecal indicator bacteria in an urban creek.this research aimed to understand the sources and fate of salmonella and fecal bacteria in urban surface waters. an urban creek (san pedro creek, california, usa) that had unusually high levels of salmonella and fecal bacteria relative to other nearby waterbodies was chosen as a model field site. state of the art microbiological methods were used in concert with modeling to investigate salmonella and fecal bacteria sources, and determine field-relevant dark inactivation and photoinactivation rat ...201121687857
mechanism of copper surface toxicity in vancomycin-resistant enterococci following wet or dry surface contact.contaminated touch surfaces have been implicated in the spread of hospital-acquired infections, and the use of biocidal surfaces could help to reduce this cross-contamination. in a previous study we reported the death of aqueous inocula of pathogenic enterococcus faecalis or enterococcus faecium isolates, simulating fomite surface contamination, in 1 h on copper alloys, compared to survival for months on stainless steel. in our current study we observed an even faster kill of over a 6-log reduct ...201121742916
automated solid phase synthesis of teichoic acids.this communication describes the first automated solid phase synthesis of teichoic acids (tas) and the preparation by this method of a number of well-defined ta structures, which were probed for their antigenicity. an opsonophagocytic killing assay revealed a clear ta-length-activity relationship and indicated a promising candidate for future vaccine development.201121734985
influence of synthetic peptide inducers on antibacterial activity of enterococci.antibacterial activity of probiotic enterococci is often related to the production of bacteriocins. there is a variety of enterococcus faecium strains which synthesise one or several enterocins including a, b, p, 96, l50ab and others. in this work we have analysed the probiotic strain e. faecium l3 whose genome contained the genes encoding enterocins ðé, ðæ and a three-component regulatory system. all these genes were found to be expressed in enterococcal strain l3. antimicrobial activity of the ...201121840807
increase of patients co-colonised or co-infected with methicillin-resistant staphylococcus aureus, vancomycin-resistant enterococcus faecium or extended-spectrum +¦-lactamase-producing enterobacteriaceae.purpose: to determine the incidence of patients co-colonised or co-infected with methicillin-resistant staphylococcus aureus (mrsa), vancomycin-resistant enterococcus faecium or extended-spectrum +¦-lactamase (esbl)-producing enterobacteriaceae in four german tertiary care hospitals. methods: this study was conducted at four tertiary care hospitals (all with >1,000 beds) in different geographic regions in germany (berlin in the east, luebeck in the north, freiburg in the southwest and nuernberg ...201121710119
genetic organization and mode of action of a novel bacteriocin, bacteriocin 51: determinant of vana-type vancomycin-resistant enterococcus faecium.bacteriocin 51 (bac 51) is encoded on the mobile plasmid phy (6,037 bp), which was isolated from vancomycin-resistant enterococcus faecium vre38. bacteriocin 51 is active against e. faecium, e. hirae, and e. durans. sequence analysis of phy showed that it encodes nine open reading frames (orfs) from orf1 to orf9 (in that order). genetic analysis suggested that orf1 and orf2, which were designated baca and bacb, respectively, are the bacteriocin and immunity genes. baca encodes a 144-amino-acid p ...201121709077
antagonistic effect of lactobacillus strains against gas-producing coliforms isolated from colicky infants.infantile colic is a common disturb within the first 3 months of life, nevertheless the pathogenesis is incompletely understood and treatment remains an open issue. intestinal gas production is thought to be one of the causes of abdominal discomfort in infants suffering from colic. however, data about the role of the amount of gas produced by infants' colonic microbiota and the correlation with the onset of colic symptoms are scanty. the benefit of supplementation with lactobacilli been recently ...201121718486
release of bacteriocins from nanofibers prepared with combinations of poly(d,l-lactide) (pdlla) and poly(ethylene oxide) (peo).plantaricin 423, produced by lactobacillus plantarum, and bacteriocin st4sa produced by enterococcus mundtii, were electrospun into nanofibers prepared from different combinations of poly(d,l-lactide) (pdlla) and poly(ethylene oxide) (peo) dissolved in n,n-dimethylformamide (dmf). both peptides were released from the nanofibers with a high initial burst and retained 88% of their original antimicrobial activity at 37 -¦c. nanofibers have the potential to serve as carrier matrix for bacteriocins a ...201121731433
characterization of certain bacterial strains for potential use as starter or probiotic cultures in dairy products.the present work was aimed at characterizing 12 strains of lactic acid bacteria (lab) to obtain improved potential starter or probiotic cultures that could be used for making dairy products from ewe's milk and cow's milk. eight strains with antimicrobial properties, isolated from ewe's milk and from cheese made from ewe's and/or cow's milk, were studied. they were identified as enterococcus faecalis (five strains), lactococcus lactis subsp. cremoris, leuconostoc mesenteroides, and lactobacillus ...201121819671
biofilm growth alters regulation of conjugation by a bacterial pheromone.conjugation is an important mode of horizontal gene transfer in bacteria, enhancing the spread of antibiotic resistance. in clinical settings, biofilms are likely locations for antibiotic resistance transfer events involving nosocomial pathogens such as enterococcus faecalis. here we demonstrate that growth in biofilms alters the induction of conjugation by a sex pheromone in e.ôçâfaecalis. mathematical modelling suggested that a higher plasmid copy number in biofilm cells would enhance a switch ...201121843206
colonization of pressure ulcers by multidrug-resistant microorganisms in patients receiving home care.abstract colonization and/or infection with multidrug-resistant microorganisms (mdro) of pressure ulcers in patients receiving care at home have seldom been investigated. the objective of this study was to assess the prevalence of mdro colonization in pressure ulcers of patients receiving home care in palermo, italy. vancomycin-resistant enterococcus (vre), methicillin-resistant staphylococcus aureus (mrsa), and multidrug-resistant gram-negative bacilli (mdrgn) were isolated, identified, and cha ...201121728745
an evaluation of antibacterial efficacy of 3% sodium hypochlorite, high-frequency alternating current and 2% chlorhexidine on enterococcus faecalis: an in vitro study.the purpose of this study was to evaluate the antibacterial efficacy of 3% sodium hypochlorite (naocl), high-frequency alternating current (hfac) (endox endodontic system), 2% chlorhexidine (chx) in elimination of enterococcus faecalis from experimentally infected root canals, in vitro.201121691496
contrasts in concentrations and loads of conventional and alternative indicators of fecal contamination in coastal stormwater.fecal contamination in stormwater is often complex. because conventional fecal indicator bacteria (fib) cannot be used to ascertain source of fecal contamination, alternative indicators are being explored to partition these sources. as they are assessed for future use, it is critical to compare alternative indicators to conventional fib under a range of stormwater delivery conditions. in this study, conventional fib and fecal bacteroides spp. were monitored throughout the duration of five storm ...201121840561
chemical composition and in vitro antibacterial activity of the essential oil of phthirusa adunca from venezuelan andes.in this paper, preliminary studies on the chemical characterization of phthirusa adunca meyer essential oil, obtained by hydrodistillation, is presented. the separation of the components was performed by gc-ms. twenty-three compounds (94.5% of the sample) were identified of which the three major ones (76% of the sample) were beta-phellandrene (38.1%), germacrene d (26.8%) and beta-pinene (11.5%). the essential oil showed a broad spectrum of activity against salmonella typhi cdc 57 (100 microg/ml ...201121834255
identification of bacteriocin genes in enterococci isolated from game animals and saltwater fish.bacteriocins produced by enterococci, referred to as enterocins, possess great interest for their potential use as biopreservatives in food and feed, as well as alternative antimicrobials in humans and animals. in this context, the aim of the present study was to determine the antimicrobial activity and the presence of bacteriocin structural genes in fecal enterococcal isolates from animal origins. evaluation of the direct antimicrobial activity of 253 isolates from wild boars (sus scrofa, n = 6 ...201121819651
lateral buttock congenital dermal sinus tract.a 6-month-old female presented with purulent discharge from a dimple in the right lateral buttock. a subcutaneous abscess was palpated on the right paravertebral region at the l5-s1 level. she had low-grade fever with laboratory findings of leukocytosis and elevation of c-reactive protein levels. klebsiella and enterococcus species were cultured from the pus. computed tomography (ct) clearly showed a tract traversing the subcutaneous tissue and connecting to the abscess. magnetic resonance (mr) ...201121701114
the challenges of antimicrobial drug resistance in greece.antimicrobial drug resistance rates in greece are among the highest in europe. the prevalence of carbapenem-resistant gram-negative species has increased considerably, including endemic strains in intensive care units. pandrug-resistant klebsiella pneumoniae, acinetobacter baumannii, and pseudomonas aeruginosa are sporadically reported. methicillin-resistant staphylococcus aureus and vancomycin-resistant enterococcus rates are also high in greek hospitals. multidrug resistance increases risk of ...201121690626
positive cytoplasmic antineutrophil cytoplasmic antigen with pr3 specificity glomerulonephritis in a patient with subacute bacterial endocarditis. 201121724732
contamination rates and antimicrobial resistance in enterococcus spp., escherichia coli, and salmonella isolated from "no antibiotics added"-labeled chicken products.abstract in the united states, products from chickens that were not administered antimicrobial medications during growout can contain labels stating "no antibiotics added." here we compared microbial profiles of chicken products labeled as coming from birds raised without antimicrobial medications (n=201; non) with chicken products carrying conventional labels (n=201; conv). there were no differences in percentages of samples positive for enterococcus spp. (conv: 17.4%; non: 21.3%) or escherichi ...201121714636
antibiotic resistance in faecal bacteria isolated from horses receiving virginiamycin for the prevention of pasture-associated laminitis.enterococcus faecium, a major cause of potentially life-threatening hospital-acquired human infections, can be resistant to several antimicrobials, such that streptogramin quinupristin-dalfopristin (q/d) is one of the few antibiotics still effective. consequently use of the streptogramin virginiamycin as an animal growth promoter was banned in the eu in 1999 as some believed this contributed to the emergence of q/d resistant e. faecium. virginiamycin is advocated for preventing equine pasture-as ...201121676560
effect of the probiotic enterococcus faecium sf68 on presence of diarrhea in cats and dogs housed in an animal shelter.beneficial effects of probiotics have never been analyzed in an animal shelter.201121689152
a rare cause of pulmonary infiltrates one should be aware of: a case of daptomycin-induced acute eosinophilic pneumonia.we report a 69-year-old patient who developed fever and dyspnea 3-áweeks after the initiation of daptomycin therapy for spondylodiscitis with lumbar epidural and bilateral psoas abscesses due to ampicillin- and high-level-gentamicin-resistant enterococcus faecium. there was profound hypoxia and the chest x-ray showed extensive patchy infiltrates bilaterally. a bronchoalveolar lavage revealed 30% eosinophils and results of microbiological studies were normal. daptomycin-induced eosinophilic pneum ...201121717147
antibiotic resistance and adhesion properties of oral enterococci associated to dental caries.abstract:201121714920
antimicrobial resistance patterns of uropathogens among children in istanbul, turkey.urinary tract infections are a common cause of end-stage renal disease in turkey. this prospective study investigated the antibiotic resistance patterns of uropathogens in order to recommend appropriate therapeutic protocols for children with urinary tract infections in istanbul, turkey. between october 2007 and october 2008, children presenting with a first episode of urinary tract infection to a pediatric outpatient clinic were enrolled in the study. urine samples were cultured, and antimicrob ...201121710858
unraveling the protein targets of vancomycin in living s. aureus and e. faecalis cells.vancomycin is a potent glycopeptide antibiotic that has evolved to specifically bind to the d-ala-d-ala dipeptide termini of nascent peptidoglycans. although this mode of action is well established, several studies indicate that vancomycin and analogues exploit noncanonical target sites. in order to address all vancomycin targets in clinically relevant staphylococcus aureus and enterococcus faecalis strains we developed a series of small-molecule photoaffinity probes based on vancomycin. proteom ...201121736328
[russian results of the international epidemiological study of clinical aspects and resistance to antimicrobial medicines of cystitis uropathogens in females (aresc): significance of empiric therapy].a total of 656 female patients from russia aged between 18 to 65 years with uncomplicated cystitis entered the international aresc trial, of them 647 were eligible for final analysis. positive cultural urine tests ( > 10(4) cfu/ml) were in 419 (64.7%) patients, 393 (93.8%) patients had monoinfection. the central laboratory of genoa (to which the isolated samples were sent) has tested 416 uropathogens from 399 patients. the following pathogens were isolated: e. coli (72.6%), enterococcus (7.0%), ...201121815455
antibiotic resistance in bacteria isolated from vegetables with regards to the marketing stage (farm vs. supermarket).the aim of this study was to elucidate whether and to what extent fresh produce from germany plays a role as a carrier and reservoir of antibiotic resistant bacteria. for this purpose, 1001 vegetables (fruit, root, bulbous vegetables, salads and cereals) were collected from 13 farms and 11 supermarkets in germany and examined bacteriologically. phenotypic resistance of enterobacter cloacae (n=172); enterobacter gergoviae (n=92); pantoea agglomerans (n=96); pseudomonas aeruginosa (n=295); pseudom ...201121700353
evaluation of the biological efficacy of hydrogen peroxide vapour decontamination in wards of an australian hospital.this study assessed the efficacy of a 'dry' hydrogen peroxide vapour decontamination in an australian hospital via a two-armed study. the in-ávivo arm examined the baseline bacterial counts in high-touch zones within wards and evaluated the efficacy of cleaning with a neutral detergent followed by either hydrogen peroxide vapour decontamination, or a manual terminal clean with bleach or det-sol 500. the in-ávitro arm examined the efficacy of hydrogen peroxide vapour decontamination on a variety ...201121824681
controlling salmonella infection in weanling pigs through water delivery of direct-fed microbials or organic acids. part i: effects on growth performance, microbial populations and immune status.pigs (n = 88) weaned at 19 -¦ 2 d of age were used in a 14-d study to evaluate the effects of water-delivered direct-fed microbials (dfm) or organic acids on growth, immune status, salmonella infection and shedding, and intestinal microbial populations following intranasal inoculation of salmonella typhimurium (s. typhimurium, 10(10 )cfu/pig). pigs were challenged with salmonella 6 d post-commencement of water treatments. treatments were: 1) control diet; 2) control diet + dfm (enterococcus faec ...201121841080
microbial dynamics during aerobic exposure of corn silage stored under oxygen barrier or polyethylene films.the aims were to compare the effects of sealing forage maize with a new oxygen barrier film or with a conventional polyethylene film. this comparison would be during both ensilage and subsequent exposure of silage to air, and would include chemical, microbiological and molecular (dna and rna) assessments. the forage was inoculated with a mixture of lactobacillus buchneri, lb. plantarum, and enterococcus faecium and ensiled in polyethylene (pe) and oxygen barrier (ob) plastic bags. the oxygen per ...201121821764
enterococcus faecalis metalloprotease compromises epithelial barrier and contributes to intestinal inflammation.matrix metalloproteases (mmps) mediate pathogenesis of chronic intestinal inflammation. we characterized the role of the gelatinase (gele), a metalloprotease from enterococcus faecalis, in the development of colitis in mice.201121699778
enterococcus faecalis sufu scaffold protein enhances sufs desulfurase activity by acquiring sulfur from its cysteine-153.iron-sulfur [fe-s] clusters are inorganic prosthetic groups that play essential roles in all living organisms. in vivo [fe-s] cluster biogenesis requires enzymes involved in iron and sulfur mobilization, assembly of clusters, and delivery to their final acceptor. in these systems, a cysteine desulfurase is responsible for the release of sulfide ions, which are incorporated into a scaffold protein for subsequent [fe-s] cluster assembly. although three machineries have been shown to be present in ...201121835272
diversity of lactic acid bacteria associated with traditional fermented dairy products in mongolia.spontaneous milk fermentation has a long history in mongolia, and beneficial microorganisms have been handed down from one generation to the next for use in fermented dairy products. the objective of this study was to investigate the diversity of lactic acid bacteria (lab) communities in fermented yak, mare, goat, and cow milk products by analyzing 189 samples collected from 13 different regions in mongolia. the lab counts in these samples varied from 3.41 to 9.03 log cfu/ml. fermented yak and m ...201121700007
delayed-onset bleb-associated endophthalmitis: presentation and outcome by culture result.to determine how culture results are associated with clinical presentations and outcomes in delayed-onset bleb-associated endophthalmitis (bae).201121691583
in vitro inactivation of endodontic pathogens with nd:yag and er:yag lasers.both nd:yag and er:yag lasers have been suggested as root canal disinfection aids. the aim of this in vitro study is to compare both wavelengths in terms of irradiation dose required for microbial inactivation, to quantify these irradiation doses and to investigate the influence of certain (laser) parameters on the antimicrobial efficacy. agar plates containing a uniform layer of enterococcus faecalis, candida albicans or propionibacterium acnes were mounted perpendicularly underneath the laser ...201121691826
the fsr quorum-sensing system of enterococcus faecalismodulates surface display of the collagen-binding mscramm ace through regulation of gele.ace, a known virulence factor and the first identified microbial surface component recognizing adhesive matrix molecule (mscramm) of enterococcus faecalisis associated with host cell adherence and endocarditis. the fsr quorum-sensing system of e. faecalis, a two-component signal transduction system, has also been repeatedly linked to virulence in e. faecalis, due in part to the transcriptional induction of an extracellular metalloprotease, gelatinase (gele). in this study, we discovered that dis ...201121705589
target region selection is a critical determinant of community fingerprints generated by 16s pyrosequencing.pyrosequencing of 16s rrna genes allows for in-depth characterization of complex microbial communities. although it is known that primer selection can influence the profile of a community generated by sequencing, the extent and severity of this bias on deep-sequencing methodologies is not well elucidated. we tested the hypothesis that the hypervariable region targeted for sequencing and primer degeneracy play important roles in influencing the composition of 16s pyrotag communities. subgingival ...201121738596
transition from parenteral to enteral nutrition induces immediate diet-dependent gut histological and immunological responses in preterm neonates.necrotizing enterocolitis (nec) in preterm infants develops very rapidly from a mild intolerance to enteral feeding into intestinal mucosal hemorrhage, inflammation, and necrosis. we hypothesized that immediate feeding-induced gut responses precede later clinical nec symptoms in preterm pigs. fifty-six preterm pigs were fed total parenteral nutrition (tpn) for 48 h followed by enteral feeding for 0, 8, 17, or 34 h with either colostrum (colos, n = 20) or formula (form, n = 31). macroscopic nec l ...201121700903
pyrosequencing-based molecular monitoring of the intestinal bacterial colonization in preterm infants.objectives:: to investigate the previously unexplored diversity of neonatal intestinal microbiota and monitor early intestinal colonization patterns in korean preterm infants using high-throughput pyrosequencing technology combined with 16s rdna- based molecular methods methods:: a total of 46,369 partial 16s rdna sequences obtained from 30 fecal samples serially taken from 10 very low birth weight preterm infants were analyzed. results:: a significant proportion of the molecular species (21.9%) ...201121734604
detection of the human specific bacteroides genetic marker provides evidence of widespread sewage contamination of stormwater in the urban environment.human sewage contamination of surface waters is a major human health concern. we found urban stormwater systems that collect and convey runoff from impervious surfaces act as a conduit for sewage originating from breeches in sanitary sewer infrastructure. a total of 828 samples at 45 stormwater outfalls were collected over a four-year period and assessed by culture based methods, pcr, and quantitative pcr (qpcr) to test for traditional and alternative indicators of fecal pollution. all outfalls ...201121689838
crystal structure of clustered regularly interspaced short palindromic repeats (crispr)-associated csn2 protein revealed ca2+-dependent double-stranded dna binding activity.clustered regularly interspaced short palindromic repeats (crispr) and their associated protein genes (cas genes) are widespread in bacteria and archaea. they form a line of rna-based immunity to eradicate invading bacteriophages and malicious plasmids. a key molecular event during this process is the acquisition of new spacers into the crispr loci to guide the selective degradation of the matching foreign genetic elements. csn2 is a nmeni subtype-specific cas gene required for new spacer acquis ...201121697083
high-level fluorescence labeling of gram-positive pathogens.fluorescence labeling of bacterial pathogens has a broad range of interesting applications including the observation of living bacteria within host cells. we constructed a novel vector based on the e. coli streptococcal shuttle plasmid pat28 that can propagate in numerous bacterial species from different genera. the plasmid harbors a promoterless copy of the green fluorescent variant gene egfp under the control of the camp-factor gene (cfb) promoter of streptococcus agalactiae and was designated ...201121731607
molecular characterization of vancomycin-resistant enterococci and extended-spectrum +¦-lactamase-containing escherichia coli isolates in wild birds from the azores archipelago.to study the prevalence of vancomycin-resistant enterococci (vre) and extended-spectrum +¦-lactamase (esbl)-containing escherichia coli isolates, and the mechanisms of resistance implicated, 220 faecal samples from wild birds were collected between 2006 and 2010 in the azores archipelago. samples were spread on slanetz-bartley agar plates supplemented with 4 mg/l vancomycin and on levine agar plates supplemented with 2 mg/l cefotaxime for vre and esbl-containing e. coli isolation, respectively. ...201121834624
antimicrobial activity of two essential oils.the aim of the study was to evaluate the antimicrobial activity of essential oils in vitro for possible application to reduce the content of microorganisms in the air of animal houses. the essential oils of cymbopogon citrarus l. and malaleuca alternifolia l. were screened against bacteria staphylococcus aureus, enterococcus faecium, pseudomonas aeruginosa, escherichia coli, proteus mirabilis and yeast candida albicans. the minimal inhibitory concentration of the active essential oils was tested ...201121739934
synthesis, antimicrobial activity and possible mechanism of action of 9-bromo-substituted indolizinoquinoline-5,12-dione derivatives.a series of 9-bromo-substituted indolizinoquinoline-5,12-dione derivatives was synthesized. antimicrobial activity assessment indicates that compounds 1, 26, 27 and 28 exhibit strong activity against gram-positive bacterial strains, including beta-hemolytic streptococcus cmcc32210, staphylococcus aureus atcc25923, staphylococcus epidermidis atcc12228, enterococcus faecalis atcc29212 and methicillin-resistant s.-áaureus atcc43300 (mrsa). compound 27 shows the best anti-mrsa activity with an mic v ...201121839550
bacteriophage-encoded shiga toxin gene in atypical bacterial host.abstract:201121733190
risk factors for positive admission surveillance cultures for methicillin-resistant staphylococcus aureus and vancomycin-resistant enterococci in a neurocritical care unit.objective:: hospitals are under increasing pressure to perform active surveillance cultures for methicillin-resistant staphylococcus aureus and vancomycin-resistant enterococcus. this study aimed to identify patients at low and high risk for positive admission surveillance cultures for methicillin-resistant staphylococcus aureus and vancomycin-resistant enterococcus in a neurocritical care unit using readily ascertainable historical factors. design:: before/after study with nested case/control s ...201121705905
efficacy of tigecycline and rifampin alone and in combination against enterococcus faecalis biofilm infection in a rat model of ureteral stent.background: we investigated the efficacy of tigecycline and rifampin alone or combined in preventing ureteral stent infection due to enterococcus faecalis. materials and methods: the activities of the two antibiotics were previously studied in-ávitro in absence or in presence of biofilm. for in vivo research, the study included a control group without bacterial challenge to-áevaluate the sterility of surgical procedure, a challenged control group that did not receive any antibiotic prophylaxis a ...201121704328
protein expression vector and secretion signal peptide optimization to drive the production, secretion, and functional expression of the bacteriocin enterocin a in lactic acid bacteria.replacement of the leader sequence (ls) of the bacteriocin enterocin a (ls(enta)) by the signal peptides (sp) of the protein usp45 (sp(usp45)), and the bacteriocins enterocin p (sp(entp)), and hiracin jm79 (sp(hirjm79)) permits the production, secretion, and functional expression of enta by different lactic acid bacteria (lab). chimeric genes encoding the sp(usp45), the sp(entp), and the sp(hirjm79) fused to mature enta plus the enta immunity genes (enta+entia) were cloned into the expression ve ...201121839785
effect of functional yogurt ny-yp901 in improving the trait of metabolic syndrome.background/objectives:this study was aimed to assess the beneficial effects on metabolic syndrome of functional yogurt ny-yp901 (namyang dairy product co. ltd and nutra r&bt inc., seoul, korea) supplemented with mixture of streptococcus thermophilus, lactobacillus acidophilus, bifidobacterium infantis and extra-ingredients containing bifidobacterium breve (cbg-c2), enterococcus faecalis fk-23, fibersol-2 and so on.subjects/methods:this study was designed as an 8-week randomized, double-blind, pl ...201121697819
streptococcal pharyngitis. 201121675907
the spectrum of infections in catheter-dependent hemodialysis patients.summarybackground and objectives catheter-dependent hemodialysis patients may develop access-related and nonaccess-related infections that may be managed in the outpatient arena or in the hospital. the goal of this study was to quantify infections in such patients, to characterize their clinical presentations, and to evaluate factors determining need for hospitalization. design, setting, participants, & measurements we collected prospective data on the clinical management of catheter-dependent h ...201121737847
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