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[leukotrienes and lipoxins]. 20092650889
study of reaction time in hemiplegics.visual reaction time and auditory reaction time (vrt and art) were measured in 25 right hemiplegics and 25 left hemiplegics in the age group of 40-60 years. there was a significant prolongation of vrt and art in both the right and left hemiplegics as compared to normals of the same age group. art following mono-aural stimulation i.e. sound input into the right and left ear separately, was also studied in the subjects. mono-aural stimulation revealed a prolongation of the art on contralateral sti ...20092634753
prevention of leprosy. 20094108289
characterization of prs5: a theta-type plasmid found in a strain of pediococcus pentosaceus isolated from wine that can be used to generate cloning vectors for lactic acid bacteria.the nucleotide sequence of prs5 (10153bp) is reported. through sequence analysis, 9 open reading frames (orfs) were identified and the following features observed: a region likely involved in replication whose structural features indicate that prs5 belongs to the pucl287 group of theta-type replicons, and hypothetical proteins putatively involved in plasmid copy number control, restriction-modification system, toxin-antitoxin system and a putative integrase. shuttle vectors for escherichia coli ...200919027788
early clinical results of the ttap acetabular cup.one hundred seventy-five threaded titanium alloy prostheses (ttap) acetabular cups and 125 cemented cups were evaluated clinically and radiographically at 1 to 3 years postoperatively. the series was statistically different in that there were more women in the cemented group than in the non-cemented group (p less than .001). the cemented group was also older than the threaded group (p less than .001). pain, ambulation, function, range of motion, and roentgenograms were evaluated in all patients. ...20092798250
early clinical results of the ttap acetabular cup.one hundred seventy-five threaded titanium alloy prostheses (ttap) acetabular cups and 125 cemented cups were evaluated clinically and radiographically at 1 to 3 years postoperatively. the series was statistically different in that there were more women in the cemented group than in the non-cemented group (p less than .001). the cemented group was also older than the threaded group (p less than .001). pain, ambulation, function, range of motion, and roentgenograms were evaluated in all patients. ...20092798250
[single photon emission computed tomography by using n-isopropyl-p-[123i]-iodoamphetamine and 99mtc-hexamethyl propyleneamine oxime in a patient with herpes simplex encephalitis].single photon emission computed tomographies (spects) by using n-isopropyl-p-[123i-imp]-iodo-amphetamine (123i-imp) and 99mtc-hexamethyl propyleneamine oxime (99mtc-hm-pao) were serially performed in a 70 yrs. old male with herpes simplex encephalitis (hse), and the chronological changes of accumulations were studied. accumulations of both 123i-imp and 99mtc-hm-pao increased initially, and then decreased with the stage of hse being advanced. the sequential appearance of the lesion in hse with 12 ...20092786584
biogenic amine content in dry fermented sausages as influenced by a producer, spice mix, starter culture, sausage diameter and time of ripening.sixteen types of dry fermented sausages were commercially produced as combinations of two producers (designated k and r), two starter cultures (pediococcus pentosaceus, c; lactobacillus curvatus+staphylococcus carnosus, f), two spicing mixtures (h; p) and two casing diameters (4.5cm, t; 7cm, w), and were sampled at days zero, 14, 28 (end of ripening), 49, 70, 91 and 112 (samples were stored at 15 degrees c and relative humidity of 70% between days 28 and 112). tyramine and putrescine content (y, ...200920416663
phenotypic and genetic heterogeneity of lactic acid bacteria isolated from "alheira", a traditional fermented sausage produced in portugal.the aim of this study was to evaluate the phenotypic and genetic heterogeneity of lactic acid bacteria (lab) isolated from "alheira", a fermented sausage produced in portugal. lab were identified to genus and species level by phenotypic characteristics, using genus or species-specific primers and sequencing of the gene encoding 16s rrna. two-hundred and eighty-three isolates were grouped into 14 species. lactobacillus plantarum was isolated from all sausages and enterococcusfaecalis from most of ...200920416703
characterization of anti-listeria bacteriocins isolated from shellfish: potential antimicrobials to control non-fermented seafood.this work had as main objectives to characterize two bacteriocins produced by lactic acid bacteria (lab) previously isolated from non-fermented seafood, in order to evaluate their potential as new food protective agents. the two bacteriocinogenic isolates were identified by polymerase chain reaction (pcr) using genus- and species-specific primers, and confirmed by 16s rdna sequencing, as enterococcus faecium and pediococcus pentosaceus. the antimicrobial spectrum of each strain included several ...200919081155
taxonomic structure and monitoring of the dominant population of lactic acid bacteria during wheat flour sourdough type i propagation using lactobacillus sanfranciscensis starters.the structure and stability of the dominant lactic acid bacterium population were assessed during wheat flour sourdough type i propagation by using singly nine strains of lactobacillus sanfranciscensis. under back-slopping propagation with wheat flour type 0 f114, cell numbers of presumptive lactic acid bacteria varied slightly between and within starters. as determined by randomly amplified polymorphic dna-pcr and restriction endonuclease analysis-pulsed-field gel electrophoresis analyses, only ...200919088320
molecular screening of wine lactic acid bacteria degrading hydroxycinnamic acids.the potential to produce volatile phenols from hydroxycinnamic acids was investigated for lactic acid bacteria (lab) isolated from spanish grape must and wine. a pcr assay was developed for the detection of lab that potentially produce volatile phenols. synthetic degenerate oligonucleotides for the specific detection of the pdc gene encoding a phenolic acid decarboxylase were designed. the pdc pcr assay amplifies a 321 bp dna fragment from phenolic acid decarboxylase. the pdc pcr method was appl ...200919099460
bio-ecological control of chronic liver disease and encephalopathy.minimal encephalopathy was originally associated with chronic liver disease but is increasingly associated with most other chronic diseases and particularly with diabetes and also chronic disorders in other organs: kidneys, lungs, thyroid and with obesity. it is increasingly with dramatically increased and more or less permanent increase in systemic inflammation, most likely a result of western lifestyle. frequent physical exercise and intake of foods rich in vitamins, antioxidants, fibres, lact ...200919104922
effect of applying molasses or inoculants containing homofermentative or heterofermentative bacteria at two rates on the fermentation and aerobic stability of corn silage.this study determined how the fermentation and aerobic stability of corn silage are affected by treatment with molasses or 2 dual-purpose inoculants applied at or above the recommended rate. corn forage (dekalb 69-70) was harvested at 39% dry matter (dm) and ensiled after treatment with no additives (control, con), molasses (mol), buchneri 500 inoculant, or pioneer 11c33 inoculant. molasses was applied at 3% of forage dm. buchneri 500 was applied at the recommended rate of 8 mg/kg fresh forage t ...200919164681
description of the microflora of sourdoughs by culture-dependent and culture-independent methods.four types of sourdoughs (l, c, b, q) from artisanal bakeries in northern italy were studied using culture-dependent and culture-independent methods. in all samples, the yeast numbers ranged from 160 to 10(7)cfu/g, and the numbers of lactic acid bacteria (lab) ranged from 10(3) to 10(9)cfu/g. the isolated lab were sequenced, and a similarity was noted between two samples (c, q), both in terms of the species that were present and in terms of the percentage of isolates. in these two samples, lacto ...200919171253
detection of arc genes related with the ethyl carbamate precursors in wine lactic acid bacteria.trace amounts of the carcinogen ethyl carbamate can appear in wine by the reaction of ethanol with compounds such as citrulline and carbamyl phosphate, which are produced from arginine degradation by some wine lactic acid bacteria (lab). in this work, the presence of arc genes for the arginine-deiminase pathway was studied in several strains of different species of lab. their ability to degrade arginine was also studied. to detect the presence of arc genes, degenerate primers were designed from ...200919219988
short communication: an evaluation of the effectiveness of lactobacillus buchneri 40788 to alter fermentation and improve the aerobic stability of corn silage in farm silos.the objective of this study was to determine if the effects of inoculation with lactobacillus buchneri 40788 were detectable when applied to whole-plant corn stored in farm silos. corn silage was randomly sampled from farms in wisconsin, minnesota, and pennsylvania, and was untreated (n = 15) or treated with an inoculant (n = 16) containing l. buchneri 40788 alone or this organism combined with pediococcus pentosaceus during may and june 2007. corn silage that was removed from the silo face duri ...200919233810
probiotics, critical illness, and methodologic bias.it has been proposed that probiotics can favorably influence the course of critically ill patients. to address this question, a limited systematic review was undertaken (medline search for articles published in english) to identify randomized, controlled trials that compared a group of critically ill patients taking probiotics with a group that did not. ten such trials, mostly with high risks of methodologic bias, were identified. when the data were combined, the probiotics did not appear to inf ...200919244148
pediocin a modulates intestinal microflora metabolism in swine in vitro intestinal fermentations.the aim of this study was to investigate in vitro the effects of pediocin a [a bacteriocin produced by lactic acid bacteria (lab) pediococcus pentosaceus fbb61] on microbial metabolism in the small and large intestine of pigs. pediocin a was partially purified by ion exchange chromatography and added to an in vitro fermentation system. the intestinal inoculum was collected from pigs immediately after slaughter, diluted with a buffer, and dispensed into fermentation syringes and vessels of the 2 ...200919251932
synthesis and antibacterial activity of nisin-containing block copolymers.nisin, an antibacterial peptide proven to be an effective inhibitor of gram-positive bacteria, was incorporated into novel block copolymer constructs and tested for retained antibacterial activity. covalent coupling was achieved by chemical modification of the n-terminal isoleucine to introduce a thiol group. thiolated-nisin derivatives were then linked to poly[ethylene oxide]-poly[propylene oxide]-poly[ethylene oxide] (peo-ppo-peo) triblocks that had been end-activated such that terminal hydrox ...200919358262
pediococcus lolii sp. nov., isolated from ryegrass silage.a gram-positive, coccus-shaped, lactic acid bacterium, strain ngri 0510q(t), was isolated from ryegrass silage produced in okinawa prefecture, japan. the cell is non-spore-forming, non-motile, and occurs in pairs or tetrads. the strain is homofermentative and produces d- and l-lactic acid from glucose. phylogenetic analysis based on 16s rrna gene sequences revealed that strain ngri 0510q(t) belongs to the genus pediococcus and clusters within the pediococcus acidilactici and pediococcus pentosac ...200919406783
bacteriocin production by pediococcus pentosaceus isolated from marula (scerocarya birrea).strain st44am, isolated from marula, was identified as pediococcus pentosaceus based on biochemical tests, sugar fermentation reactions (api 50chl), pcr with species-specific primers and 16s rdna sequencing. strain st44am produces a 6.5 kda class iia bacteriocin, active against lactic acid bacteria, escherichia coli, pseudomonas aeruginosa, klebsiella pneumoniae, listeria innocua, listeria ivanovii subsp. ivanovii and listeria monocytogenes. the peptide is inactivated by proteolytic enzymes, but ...200919446352
screening, selection and characterization of phytic acid degrading lactic acid bacteria from chicken intestine.this study was undertaken to screen and select potent phytate degrading lactic acid bacteria and to evaluate their additional characteristic features. forty lactic acid bacterial strains were isolated from different sources and screened for their ability to degrade myo-inositol hexaphosphate or ip(6) by cobalt chloride staining (plate assay) method, using calcium or sodium salt of phytic acid as substrate. all the forty isolates were able to degrade calcium phytate. however, only two pediococcus ...200919481282
inactivation of oenological lactic acid bacteria (lactobacillus hilgardii and pediococcus pentosaceus) by wine phenolic compounds.to investigate the inactivation properties of different classes of phenolic compounds present in wine against two wine isolates of lactobacillus hilgardii and pediococcus pentosaceus, and to explore their inactivation mechanism.200919486417
lactic acid bacteria as reducing and capping agent for the fast and efficient production of silver nanoparticles.there is a growing demand for silver-based biocides, including both ionic silver forms and metallic nanosilver. the use of metallic nanosilver, typically chemically produced, faces challenges including particle agglomeration, high costs, and upscaling difficulties . additionally, there exists a need for the development of a more eco-friendly production of nanosilver. in this study, gram-positive and gram-negative bacteria were utilized in the non-enzymatic production of silver nanoparticles via ...200919488750
polyphasic taxonomic studies of lactic acid bacteria associated with tunisian fermented meat based on the heterogeneity of the 16s-23s rrna gene intergenic spacer region.the objective of this work was to investigate the structure and diversity of lactic acid bacteria (lab) communities in traditionally fermented meat collected from different areas of tunisia. a polyphasic study, which involves phenotypic tests and ribosomal dna-based techniques, was used to identify gram-positive and catalase-negative isolates. pcr amplification of the 16s-23s rdna isr of 102 isolates and other reference lab strains gave (1) one type of rrn operon (m-isr) for lactococci, (2) two ...200919669730
identification of lactic acid bacteria isolated from tarhana, a traditional turkish fermented food.tarhana is a traditional fermented product produced from a mixture of spontaneously fermented yogurt and wheat flour in turkey. the aims of the present study were to enumerate and identify for the first time by molecular biology-based methods predominant lactic acid bacteria (lab) isolated during processing of tarhana. samples were collected from eight different regions of turkey. in order to explore the relationship between raw material and the microbiology of tarhana, yogurt and wheat flour we ...200919703719
the development of lactic acid bacteria and lactobacillus buchneri and their effects on the fermentation of alfalfa silage.this study was conducted to document the development of populations of lactic acid bacteria (lab) and lactobacillus buchneri in alfalfa silage treated with various inoculants. wilted and chopped alfalfa (45% dry matter) was treated with 1) distilled water (untreated, u), 2) lactobacillus buchneri 40788 (4 x 10(5) cfu/g; lb), or 3) l. buchneri 40788 (4 x 10(5) cfu/g) and pediococcus pentosaceus (1 x 10(5) cfu/g; lbpp). forages were packed into triplicate vacuum-sealed, nylon-polyethylene bags per ...200919762819
pediocin a improves growth performance of broilers challenged with clostridium perfringens.the aim of this study was to investigate the efficacy of the anticlostridial pediocin a from pediococcus pentosaceus fbb61 to contain negative effects associated to clostridium proliferation in broilers, through 2 subsequent investigations. in the first study, 36 ross 508 broilers were divided into 3 groups and fed for 21 d as follows: the control diet (ctr), the control diet supplemented with supernatant filtrate of a culture of p. pentosaceus fbb61-2 (bac-, isogenic mutant nonproducing pedioci ...200919762869
evaluation of lactic acid bacteria for sourdough fermentation of amaranth.spontaneous fermented sourdoughs prepared from five amaranth flours were investigated for the presence of lactic acid bacteria predominating the autochthonous microbiota and thus may be suitable as starter cultures. the doughs were fermented with daily back-slopping on a laboratory scale at 30 degrees c for 10 days. each day, ph-values and total titratable acidity degrees were determined and samples were analyzed for lactic acid bacteria and yeasts by cultural methods. the identity of the strain ...200919783060
development of a pcr assay for the strain-specific identification of probiotic strain lactobacillus paracasei impc2.1.recent investigations clearly indicate that the probiotic bacterium lactobacillus paracasei impc2.1 can be incorporated into vegetables to obtain innovative probiotic foods whose marketing has been authorized by the italian ministry of health. in this study, strain impc2.1 was characterized at a molecular level in order to define its taxonomic position and to develop a pcr test for strain-specific identification. molecular methods, such as 16s rrna gene sequencing and multiplex pcr, have provide ...200919833402
studies on oxidized low density lipoproteins. controlled oxidation and a prostaglandin artifact.low density lipoproteins (ldl), isolated by ultracentrifugal flotation, were oxidized (ldloxid) slowly during dialysis against 0.15 m nacl and subsequent incubation in 96% air-4% co2 at 37 degrees c. butylated hydroxytoluene prevented ldl oxidation. ldl preparations from different sera were oxidized at different rates and the degree of lipid peroxidation was controlled by varying the incubation time. mild oxidation did not alter the electrophoretic mobility of the ldloxid preparations. ldloxid c ...20092715720
[strabismus in children]. 20082620724
[strabismus in children]. 20082620724
diagnosis of infectious mononucleosis. 20082592913
the polymorphic oxidation of beta-adrenoceptor antagonists. 20082566179
pcr amplification of dna microdissected from a single polytene chromosome band: a comparison with conventional microcloning.a novel alternative to microcloning for the production of region specific chromosomal dna is described. in this method, 'microamplification', single bands are dissected from polytene chromosomes and digested with sau3a. oligonucleotide adaptors are ligated to these fragments to provide convenient priming sites for polymerase chain reaction amplification. in this way, as much as 1 microgram of dna can be amplified from a single band. probes made from pcr amplified dna from two such dissections ha ...20082587252
[multiple sclerosis in health care in our country: electrophysiological, immunological and clinical analysis].among 70 patients referred for suspected multiple sclerosis (ms) a total of 40 met criteria for definite (17), likely (12) or possible (11) diagnosis of the disease. visual evoked potentials provided evidence of cns lesion in 13 cases. abnormalities of somatosensory evoked potentials, particularly on stimulation of the posterior tibial nerve, were present in 90% of patients. brainstem auditory evoked potentials were of little use, contributing to diagnosis of one subclinical lesion; they were no ...20082519355
ecological removal of recalcitrant phenolic compounds of treated olive mill wastewater by pediococcus pentosaceus.treatment of olive mill wastewater by different biological process led to an important decrease of organic compounds but the black coloration due to the recalcitrant phenolic compounds persists. experimental design using fractional factorial plan showed that, among the seven studied factors, yeast extract, dilution and glucose exhibited a positive effect on pediococcus pentosaceus growth and treated olive mill wastewater (tomw) decolourisation. optimization of influent factors showed that 2.5 an ...200817686628
screening from pickled vegetables the potential probiotic strains of lactic acid bacteria able to inhibit the salmonella invasion in mice.to screen from pickled vegetables the potential probiotic lactic acid bacteria (lab) strains with antagonistic activity against salmonella invasion in host.200817927755
lactic acid bacteria with potential to eliminate fungal spoilage in foods.to investigate antifungal activity produced by lactic acid bacteria (lab) isolated from malted cereals and to determine if such lab have the capacity to prevent fungal growth in a particular food model system.200817976175
role of hypermutability in the evolution of the genus oenococcus.oenococcus oeni is an alcohol-tolerant, acidophilic lactic acid bacterium primarily responsible for malolactic fermentation in wine. a recent comparative genomic analysis of o. oeni psu-1 with other sequenced lactic acid bacteria indicates that psu-1 lacks the mismatch repair (mmr) genes muts and mutl. consistent with the lack of mmr, mutation rates for o. oeni psu-1 and a second oenococcal species, o. kitaharae, were higher than those observed for neighboring taxa, pediococcus pentosaceus and l ...200817993526
isolation and characterization of bacteriocin-producing bacteria from the gastrointestinal tract of broiler chickens for probiotic use.to isolate and characterize the bacteriocin-producing bacteria (bpb) from the gastrointestinal tract of broiler chickens for probiotic use.200819120666
functionality of lactic acid bacteria peptidase activities in the hydrolysis of gliadin-like fragments.to evaluate the role of the peptidase activities from sourdough lactic acid bacteria (lab) in the degradation of alpha-gliadin fragments.200819146533
phenotypic and genotypic identification of lactic acid bacteria isolated from ethnic fermented bamboo tender shoots of north east india.mesu, soidon, soibum and soijim are ethnic fermented bamboo tender shoot products prepared by the people in north east india. microbiological analysis of mesu, soidon, soibum and soijim showed the population dominated by lactic acid bacteria (lab) ranging up to 10(8) cfu g(-1). the phenotypic characterisation of predominant lab isolated from the fermented bamboo shoot products was based on general morphology, physiological tests, api and biolog systems. the genotypic characterisation of lab was ...200818036695
method for reliable isolation of lactobacillus sakei strains originating from tunisian seafood and meat products.in tunisia, several food products derived from meat or seafood are naturally processed, without any addition of bacterial starters. such fermented, dried-cured, salted, or marinated products, as well as the raw meat or fish may thus provide a source to isolate the natural microflora colonizing such environments. we isolated lactic acid bacteria from a representative range of flesh-foods sold or manufactured in different parts of tunisia, and selectively searched for lactobacillus sakei, a lactic ...200818155310
population diversity of yeasts and lactic acid bacteria in pig feed fermented with whey, wet wheat distillers' grains, or water at different temperatures.the diversity of populations of yeast and lactic acid bacteria (lab) in pig feeds fermented at 10, 15, or 20 degrees c was characterized by rrna gene sequencing of isolates. the feeds consisted of a cereal grain mix blended with wet wheat distillers' grains (wwdg feed), whey (w feed), or tap water (wat feed). fermentation proceeded for 5 days without disturbance, followed by 14 days of daily simulated feed outtakes, in which 80% of the contents were replaced with fresh feed mixtures. in wwdg fee ...200818223110
nisin antimicrobial activity and structural characteristics at hydrophobic surfaces coated with the peo-ppo-peo triblock surfactant pluronic f108.the antimicrobial peptide nisin has been observed to preferentially locate at surfaces coated with the poly[ethylene oxide]-poly[propylene oxide]-poly[ethylene oxide] (peo-ppo-peo) surfactant pluronic f108, to an extent similar to its adsorption at uncoated, hydrophobic surfaces. in order to evaluate nisin function following its adsorption to surfaces presenting pendant peo chains, the antimicrobial activity of nisin-loaded, f108-coated polystyrene microspheres and f108-coated polyurethane cathe ...200818377919
inhibition of escherichia coli o157:h7 in ripening dry fermented sausage by ground yellow mustard.compounds generated by the enzymatic hydrolysis of glucosinolates naturally present in mustard powder are potently bactericidal against escherichia coli o157:h7. because e. coli o157:h7 can survive the dry fermented sausage manufacturing process, 2, 4, and 6% (wt/wt) nondeheated (hot) mustard powder or 6% (wt/wt) deheated (cold) mustard powder were added to dry sausage batter inoculated with e. coli o157:h7 at about 7 log cfu/g to evaluate the antimicrobial effectiveness of the powders. reductio ...200818389690
transport of glycerol by pediococcus pentosaceus isolated from wine.pediococcus pentosaceus n(5)p is a strain isolated from wine that uses glycerol as its sole carbon source, mainly via the glycerol kinase pathway. the transport of glycerol was investigated in resting cells of this strain. glycerol uptake followed a michaelis-menten relationship with an observed apparent k(m) of 33 microm and a v(max) of 2.5 nmol/min/mg of cell protein. the transport system was specific for glycerol, which was present in the cells grown either on glycerol or glucose suggesting i ...200818456108
isolation and partial characterization of a bacteriocin produced by pediococcus pentosaceus k23-2 isolated from kimchi.screening and partial characterization of a bacteriocin produced by pediococcus pentosaceus k23-2 isolated from kimchi, a traditional korean fermented vegetable.200818540969
a factory-scale application of secondary adjunct cultures selected from lactic acid bacteria during puzzone di moena cheese ripening.the lactic acid populations of 2 seasonal puzzone di moena cheeses made from winter and summer raw cow's milk were characterized at different ripening times. lactic acid bacteria (lab) were isolated on selective media and subjected to genetic typing and identification. the species most frequently found during ripening were lactobacillus paracasei ssp. paracasei, lactobacillus plantarum, and pediococcus pentosaceus. the different strains recognized by random amplification of polymorphic dna-pcr w ...200818650274
pediococcus pentosaceus nb-17 for probiotic use.the plant-derived pediococcus pentosaceus nb-17 was isolated from japanese traditional vegetable pickles. the production of cytokines from mouse spleen cells co-cultivated with heat-killed bacteria was investigated in vitro. the bacteria significantly induced secretion levels of interferon (ifn)-gamma and interleukin (il)-12 p70, and suppressed il-4 productions in ovalbumin (ova) sensitized mouse spleen cells. therefore, the bacteria could effectively stimulate immune activities and showed aller ...200818691534
selection and use of autochthonous mixed starter for lactic acid fermentation of carrots, french beans or marrows.strains of leuconostoc mesenteroides, lactobacillus plantarum, weissella soli/weissella koreensis, enterococcus faecalis, pediococcus pentosaceus and lactobacillus fermentum were identified from raw carrots, french beans and marrows by partial 16s rrna gene sequence. l. plantarum m1, leuc. mesenteroides c1 and p. pentosaceus f4 were selected based on the rates of growth and acidification in vegetable juice media, and used as the autochthonous mixed starter for the fermentation of carrots, french ...200818710789
diversity of lactic acid bacteria in two flemish artisan raw milk gouda-type cheeses.pcr-denaturing gradient gel electrophoresis (pcr-dgge) was used to study the diversity of lactic acid bacteria (lab) in two flemish artisan raw milk gouda-type cheeses. in parallel, conventional culturing was performed. isolates were identified using (gtg)(5)-pcr and sequence analysis of 16s rrna and phes genes. discriminant analysis revealed some differences in overall lab diversity between the two batches and between the two cheeses. within each batch, the diversity of 8- and 12-week-old chees ...200818721684
microbial diversity of traditional vietnamese alcohol fermentation starters (banh men) as determined by pcr-mediated dgge.the diversity of fungi and bacteria associated with traditional vietnamese alcohol fermentation starters (banh men) was investigated by pcr-mediated dgge. from 52 starter samples, 13 species of fungi (including yeasts) and 23 species of bacteria were identified. the fungal composition of the starters was consistent with little variation among samples. it consisted of amylase producers (rhizopus oryzae, r. microsporus, absidia corymbifera, amylomyces sp., saccharomycopsis fibuligera), ethanol pro ...200818838186
the aps knee joint prosthesis. a review of 32 patients.first clinical and radiographic results with a new knee joint prosthesis, implanted without cement, are reported and the characteristics of the implant (dowel fixation and special instrumentation) presented. the first series of 35 joints with a follow-up period of 1-3 years shows excellent results. radiographically, primary osseous integration cannot be positively identified in all cases, but it could be proved in most instances. cement-free implantation requires good preoperative bone quality a ...20082271356
pediococcus argentinicus sp. nov. from argentinean fermented wheat flour and identification of pediococcus species by phes, rpoa and atpa sequence analysis.a gram-positive, small coccus-shaped lactic acid bacterium, strain lmg 23999(t), was isolated from argentinean wheat flour. 16s rrna gene sequence analysis revealed that the phylogenetic position of the novel strain was within the genus pediococcus, with pediococcus stilesii, pediococcus pentosaceus and pediococcus acidilactici as its closest relatives (97.7, 97.3 and 96.9 % gene sequence similarity, respectively). fluorescent amplified fragment length polymorphism fingerprinting of whole genome ...200819060081
molecular and technological characterization of lactic acid bacteria from traditional fermented sausages of basilicata region (southern italy).lactic acid bacteria (lab) from traditional fermented sausages of the basilicata region were investigated by ardra-pcr and rapd-pcr for taxonomic identification at species and strain level and characterized on the basis of the growth and acidification at different temperatures, incubation times, levels of nacl and kno(2), hydrolysis of sarcoplasmatic and myofibrillar proteins and antimicrobial, peptide/amino acid release and nitrate reductase activities. lactobacillus sakei was the predominant s ...200822063864
effect of processing methods and starter culture (staphylococcus xylosus and pediococcus pentosaceus) on proteolytic changes in turkish sausages (sucuk) during ripening and storage.proteolytic changes in turkish sausages (sucuk) produced by two methods (heat processing and traditional) were determined during processing and storage for 90 days. the sausages were produced with or without starter culture in both methods. a mixture of staphylococcus xylosus and pediococcus pentosaceus was used as starter culture for their acidic and proteolytic characteristics. the major changes in proteolytic characteristics of sucuk took place during the fermentation stage, with an increase ...200822063339
outpatient geriatric evaluation.this article provides a comprehensive description of an established geriatric ambulatory consultation service at monroe community hospital in rochester, new york. the history of the service is presented and clinic staffing and operations are reviewed. descriptive information for patients evaluated during a 1-year period and the impact of this team-oriented assessment compared to assessment by physicians are provided.20083815242
prolongation of anesthesia with xylazine, ketamine, and guaifenesin in horses: 64 cases (1986-1989).on 74 occasions, 54 horses and 6 foals were anesthetized with xylazine and ketamine or xylazine, guaifenesin, and ketamine, with or without butorphanol. on 64 occasions, anesthesia was prolonged for up to 70 minutes (34 +/- 15 min) by administration of 1 to 9 supplemental iv injections of xylazine and ketamine at approximately a third the initial dosage. all horses except 5 were positioned in lateral recumbency, and oxygen was insufflated. in adult horses, the time from induction of anesthesia t ...20082276967
the next wave: the combination of clinical work with the opportunity to serve people makes dentistry an attractive career option for women. 20082605768
hemodynamic and electrophysiologic effects of amlodipine, a new long-acting calcium antagonist: preliminary observations. 20082530867
induction of transient hyperglycaemia in cancer patients.serum glucose levels up to 700 mg/dl were achieved and maintained for 3 h in patients with advanced metastatic cancer by infusion of concentrated glucose solutions. even with infusions of large amounts of glucose, the maximum sustainable level of serum glucose did not exceed 700 mg/dl. the apparent ceiling of serum glucose level appeared to be due to a large and rapid loss of glucose from the kidneys. with adequate fluid volume replacement there was no rebound hypoglycaemia and there was no sign ...20082394924
adolescent medicine. 20082329647
new prosthetic venous collar anastomotic technique: combining the best of other procedures. 20071913097
[leukocyte and atherosclerosis]. 20071947989
mucinous ascites associated with rupture of benign ovarian teratoma. case report. 20072223689
mucinous ascites associated with rupture of benign ovarian teratoma. case report. 20072223689
[pregnancy and cystic fibrosis. a case history].the authors report a case of pregnancy in a patient suffering from moderately severe cystic fibrosis (the syndrome consisted of diffused obstruction in respiratory function with a vital capacity of 79% and the lowered blood oxygen level with arterial blood gas estimations; oxygen saturation was 94%). the condition was marked by temporary worsening during the pregnancy in which infective bronchitis supervened). she also threatened to go into early labour and did in fact deliver at 36 1/2 weeks of ...20072258595
hospital outbreak of scabies stemming from two aids patients with norwegian scabies. 20071971073
post-concussional symptoms following minor head injury.the effect of admission to hospital after a minor head injury on the incidence and duration of post-concussional symptoms was investigated. a total of 114 adults with a minor head injury were randomly allocated to admission for 24 h or to discharge from the accident department. follow-up was by postal questionnaire. this revealed that 90 per cent of patients suffered symptoms lasting an average of 2 weeks. the patients who were discharged had symptoms that lasted a shorter time. admission to hos ...20072592090
antilisterial activity of lactic acid bacteria isolated from "alheiras" (traditional portuguese fermented sausages): in situ assays.a total of 226 lactic acid bacteria (lab) isolated from "alheira", a traditional portuguese fermented sausage, were screened for antagonistic activity against some pathogenic microorganisms, including listeria monocytogenes. the objective was to isolate lab with antibacterial activity from "alheiras" and to select strains that could be used in "alheira" production. isolates displaying antibacterial activity against listeria innocua and l. monocytogenes were investigated for the nature of the ant ...200722061260
characterization and selection of autochthonous lactic acid bacteria isolated from traditional iberian dry-fermented salchichón and chorizo sausages.a total of 192 lactic acid bacteria were isolated from 2 types of naturally fermented dry sausages at 3 different stages of the ripening process in order to select the most suitable strains as starter cultures in dry-cured sausage manufacture according to their technological characteristics such as glucose fermentation, lactic and acetic acid production, and proteolytic, lipolytic, and antimicrobial activities. identification of the isolates revealed that 31.2% were pediococcus pentosaceus, 26.9 ...200717995686
microbial development in distillers wet grains produced during fuel ethanol production from corn (zea mays).distillers grains are coproduced with ethanol and carbon dioxide during the production of fuel ethanol from the dry milling and fermentation of corn grain, yet there is little basic microbiological information on these materials. we undertook a replicated field study of the microbiology of distillers wet grains (dwg) over a 9 day period following their production at an industrial fuel ethanol plant. freshly produced dwg had a ph of about 4.4, a moisture content of about 53.5% (wet mass basis), a ...200718026225
redox potential to discriminate among species of lactic acid bacteria.to verify to what degree reducing capacity is a characterizing parameter of a species, and of the strains themselves within a given species, of lactic acid bacteria.200717953562
a survey of the microbial and chemical composition of seven semi-ripened provola dei nebrodi sicilian cheeses.the microbial and chemical composition of seven different semi-ripened (45 days) provola dei nebrodi sicilian cheese samples were assessed in order to investigate the diversity of the microbial population in cheese made from different geographical areas throughout sicily.200717897218
dna fingerprinting of lactic acid bacteria in sauerkraut fermentations.previous studies using traditional biochemical identification methods to study the ecology of commercial sauerkraut fermentations revealed that four species of lactic acid bacteria, leuconostoc mesenteroides, lactobacillus plantarum, pediococcus pentosaceus, and lactobacillus brevis, were the primary microorganisms in these fermentations. in this study, 686 isolates were collected from four commercial fermentations and analyzed by dna fingerprinting. the results indicate that the species of lact ...200717921264
survival of escherichia coli o157:h7 in dry fermented sausages containing micro-encapsulated probiotic lactic acid bacteria.escherichia coli o157:h7 is capable of surviving the rigorous processing steps during the manufacture of dry fermented sausages. the effect of adding two probiotic organisms, lactobacillus reuteri and bifidobacterium longum as co-cultures with the meat starter cultures pediococcus pentosaceus and staphylococcus carnosus on the viability of e. coli o157:h7 in dry fermented sausages was studied. a 5 strain cocktail of e. coli o157:h7 was added at 7.4 log cfu/g to the sausage batter and challenged ...200716943098
antimicrobial susceptibility of non-enterococcal intrinsic glycopeptide-resistant gram-positive organisms.non-enterococcal gram-positive bacteria that are intrinsically vancomycin-resistant have been infrequently isolated in association with serious infections. however, well-documented infections have lately been reported with increasing frequency. because these organisms may be pathogens, we tested the mics of 19 antimicrobial agents by the agar dilution method for predicting susceptibility. the activity of these antimicrobial agents was assessed against 28 strains (lactobacillus rhamnosus, 6; lact ...200717049799
susceptibility of pediococcus spp. to antimicrobial agents.to investigate the susceptibility of pediococcus species to antimicrobial agents.200717241343
identification of subdominant sourdough lactic acid bacteria and their evolution during laboratory-scale fermentations.presumptive lactic acid bacterial cocci were found in six sourdoughs (out of 20) from the abruzzo region (central italy) and subjected to phenotypic and genotypic characterization. a total of 21 isolates, recognized as seven strains by randomly amplified polymorphic dna (rapd)-polymerase chain reaction (pcr) typing, were identified by a polyphasic approach, consisting of 16s rrna gene sequencing, multiplex pcr assays and physiological features, as enterococcus faecium and pediococcus pentosaceus ...200717418310
pretreatment with pro- and synbiotics reduces peritonitis-induced acute lung injury in rats.to study whether enteral pretreatment with a synbiotic composition of lactic acid bacteria and bioactive fibers can reduce peritonitis-induced lung neutrophil infiltration and tissue injury in rats.200717426542
lactic acid bacteria and yeasts associated with gowé production from sorghum in bénin.to identify the dominant micro-organisms involved in the production of gowé, a fermented beverage, and to select the most appropriate species for starter culture development.200717650194
detection of antimicrobial activities and bacteriocin structural genes in faecal enterococci of wild animals.the production of antimicrobial activities as well as the presence of bacteriocin structural genes (enta, entb, entp, entq, cyll, entas-48, bac31, and entl50a/b) were studied in 140 non-selected faecal enterococcal isolates recovered from wild animals. eight different indicator strains (including listeria monocytogenes, pediococcus pentosaceus, and different enterococcal species) were used for antimicrobial activity detection. twenty-five of the 140 enterococci (18%) showed antimicrobial activit ...200716872815
no evidence for the enhanced production of insulin autoantibodies after confrontation with common viral antigens in insulin dependent diabetes mellitus.the production of insulin autoantibodies (iaa) was studied after common viral infections in 12 children with type 1 diabetes mellitus and in their 18 healthy siblings. in addition, the production of iaa was measured after influenza vaccination with booster in 39 patients with type 1 diabetes mellitus and in 39 healthy controls. in 7 of the 12 diabetic children 13 viral infections were serologically confirmed. among the siblings 14 periods of infection were noted in 9 individuals. a significant r ...20072074915
[biochemistry of mitochondrial encephalomyopathy]. 20072223265
[biochemistry of mitochondrial encephalomyopathy]. 20072223265
acid and the pain of non-ulcer dyspepsia. 20071932675
[a case illustrating diagnostic difficulties in bronchiolo- alveolar cancer]. 20072168044
[familial juvenile nephronophthisis]. 20072075033
[case report of successful surgical treatment of a duplicate aortic arch in an elderly woman]. 20073450109
isolated significant left main coronary artery stenosis: angiographic, hemodynamic, and clinical findings in 16 patients.among 21,545 adult patients who underwent consecutive coronary angiography, 16 (0.07%) were found during their coronary arteriography to have a significant isolated stenotic lesion (luminal diameter narrowing of 50% or more) located at the left main coronary artery. the remaining major epicardial coronary arteries and their branches were free of disease. a strong predilection for the isolated lesion to occur at the ostium of the left main artery was found (12 patients). the most common presentin ...20071950993
violent trends. 20072034554
[fractures-dislocations of the shoulder in middle-aged and elderly patients].114 patients with fracture dislocations of the proximal end of humerus have been treated and observed. 83 of them were subjected to conservative and 31--to surgical treatment. clinical, roentgenologic and radiologic evaluation of the immediate and long-term results allowed to formulate recommendations as to the selection of the treatment method of humerus fracture dislocations depending upon their type and general state of the patients.20072074957
lactic acid bacteria in hamei and marcha of north east india.hamei and marcha are mixed dough inocula used as starters for preparation of various indigenous alcoholic beverages in manipur and sikkim in india, respectively. these starters are traditionally prepared from rice with wild herbs and spices. samples of hamei and marcha, collected from manipur and sikkim, respectively, were analysed for lactic acid bacterial composition. the population of lactic acid bacteria (lab) was 6.9 and 7.1 log cfu/g in hamei and marcha, respectively. on the basis of pheno ...200723100653
thoracic actinomycosis--review of 9 cases.nine cases of thoracic actinomycosis treated in the period from 1976 to 1990 were reviewed. among them, 4 cases were children. the clinical symptoms and laboratory examinations were non-specific. chest roentgenographic manifestations including nodular (3 cases), infiltrations (4 cases), and infiltrations with pleural changes (2 cases) were also non-specific. chest wall masses were noted in four patients. there were no cases correctly diagnosed on admission. the diagnosis of thoracic actinomycosi ...20061797368
thoracic actinomycosis--review of 9 cases.nine cases of thoracic actinomycosis treated in the period from 1976 to 1990 were reviewed. among them, 4 cases were children. the clinical symptoms and laboratory examinations were non-specific. chest roentgenographic manifestations including nodular (3 cases), infiltrations (4 cases), and infiltrations with pleural changes (2 cases) were also non-specific. chest wall masses were noted in four patients. there were no cases correctly diagnosed on admission. the diagnosis of thoracic actinomycosi ...20061797368
medical waste: science vs politics. 20061539964
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