Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| random amplified polymorphic dna-pcr based cloning of markers to identify the beer-spoilage strains of lactobacillus brevis, pediococcus damnosus, lactobacillus collinoides and lactobacillus coryniformis. | beer-spoilage ability of lactic acid bacteria such as lactobacillus brevis is a strain-dependent phenomenon in which the mechanism has not yet been completely clarified. in order to systematically identify genes that contribute to beer-spoilage, large-scale random amplified polymorphic dna (rapd)-based cloning methods was carried out. | 2005 | 15836491 |
| biodiversity of lactobacillus sanfranciscensis strains isolated from five sourdoughs. | five different sourdoughs were investigated for the composition of lactic acid bacteria (lab) and the biodiversity of lactobacillus sanfranciscensis strains. | 2005 | 15836738 |
| reclassification of pediococcus urinaeequi (ex mees 1934) garvie 1988 as aerococcus urinaeequi comb. nov. | the taxonomic status of pediococcus urinaeequi is described, and the transfer of the species to the genus aerococcus with the name aerococcus urinaeequi comb. nov. is proposed, on the basis of the analysis of 16s rrna gene sequence and dna-dna hybridization data. | 2005 | 15879276 |
| use of hydrolysates from atlantic cod (gadus morhua l.) viscera as a complex nitrogen source for lactic acid bacteria. | hydrolysates of cod viscera were tested as an alternative to commonly used complex nitrogen sources (peptones and/or extracts) for the type strains of the lactic acid bacteria lactococcus lactis, lactobacillus acidophilus, lactobacillus helveticus, lactobacillus casei, lactobacillus sakei and pediococcus pentosaceus. comparative studies with mrs-like media containing different nitrogen sources showed that all the fish hydrolysates performed equally well or better than commercial extracts/peptone ... | 2005 | 15941626 |
| development of solution phase hybridisation pcr-elisa for the detection and quantification of enterococcus faecalis and pediococcus pentosaceus in nurmi-type cultures. | nurmi-type cultures (ntcs), derived from the fermentation of caecal contents of specifically pathogen-free (spf) birds, have been used successfully to control salmonella colonisation in chicks. these cultures are undefined in nature and, consequently, it is difficult to obtain approval from regulatory agencies for their use as direct fed microbials (dfms) for poultry. progress towards the generation of effective defined probiotics requires further knowledge of the composition of these cultures. ... | 2005 | 15949857 |
| cloning and expression of the malolactic gene of pediococcus damnosus ncfb1832 in saccharomyces cerevisiae. | wine production is characterized by a primary alcoholic fermentation, conducted by saccharomyces cerevisiae, followed by a secondary malolactic fermentation (mlf). although most lactic acid bacteria (lab) have the ability to metabolize l-malate, only a few species survive the high ethanol and so2 levels in wine. wines produced in colder viticultural regions have a lower ph than wines produced in warmer regions. the decarboxylation of l-malate in these wines leads to an increase in ph, more organ ... | 2005 | 15950306 |
| assessment of the effects of nurmi-type cultures and a defined probiotic preparation on a salmonella typhimurium 29e challenge in vivo. | the effects of treatment with an undefined commercial nurmi-type culture (ntc), cultured cecal contents, and a dual-strain probiotic, containing enterococcus faecalis and pediococcus pentosaceus, on salmonella typhimurium colonization were evaluated in a specific-pathogen-free bird model. two sets of trials were performed, and each study was arranged as a randomized complete block design with three treatments. treatments consisted of (i) control, (ii) commercial ntc, and (iii) cultured cecal con ... | 2005 | 15954712 |
| use of an alpha-galactosidase gene as a food-grade selection marker for streptococcus thermophilus. | the alpha-galactosidase gene (aga) of lactococcus raffinolactis atcc 43920 was previously shown to be an efficient food-grade selection marker in lactococcus lactis and pediococcus acidilactici but not in streptococcus thermophilus. in this study, we demonstrated that the alpha-galactosidase of l. raffinolactis is thermolabile and inoperative at 42 degrees c, the optimal growth temperature of s. thermophilus. an in vitro assay indicated that the activity of this alpha-galactosidase at 42 degrees ... | 2005 | 15956297 |
| cloning vectors based on cryptic plasmids isolated from lactic acid bacteria: their characteristics and potential applications in biotechnology. | lactic acid bacteria (lab) are gram positive bacteria, widely distributed in nature, and industrially important as they are used in a variety of industrial food fermentations. the use of genetic engineering techniques is an effective means of enhancing the industrial applicability of lab. however, when using genetic engineering technology, safety becomes an essential factor for the application of improved lab to the food industry. cloning and expression systems should be derived preferably from ... | 2004 | 15707158 |
| tannase activity by lactic acid bacteria isolated from grape must and wine. | we examined a range of oenological lactic acid bacteria species and reference strains for their potential to degrade tannins. bacterial tannase activity was checked by a spectrophotometric and a visual reading method. none of the strains belonging to the oenological species of the genus lactobacillus, leuconostoc, oenococcus or pediococcus were tannase producers, with the exception of lactobacillus plantarum. all the l. plantarum strains analyzed were positive for tannase activity and their iden ... | 2004 | 15364474 |
| the passage of lactic acid bacteria from silage into rumen fluid, in vitro studies. | inoculated silages sometimes improve cattle performance, possibly because of probiotic effects of lactic acid bacteria (lab) silage inoculants. the cause of improved animal performance following feeding with inoculated silage is unclear. one issue in studying this phenomenon is to find out whether lab pass from silage into the rumen fluid and survive in it. the purpose of the present study was to determine whether lab from inoculated and uninoculated silages pass into the rumen fluid in vitro. w ... | 2004 | 15377617 |
| the influence of treatment with dual purpose bacterial inoculants or soluble carbohydrates on the fermentation and aerobic stability of bermudagrass. | this study determined the effectiveness of an inoculant (bb), molasses, or a mixture of either bb and molasses (bbm) or bb and fibrolytic enzymes (bbe) for improving the fermentation and aerobic stability of bermudagrass. a 6-wk regrowth of tifton 85 bermudagrass was conserved in quadruplicate mini silos alone or after treatment application. the inoculant contained a mixture of p. pentosaceus 12455, 1 x10(5) cfu/g of fresh forage, l. buchneri 40788, 4 x10(5) cfu/g of fresh forage, and beta-gluca ... | 2004 | 15377619 |
| antimicrobial potential of immobilized lactococcus lactis subsp. lactis atcc 11454 against selected bacteria. | immobilization of living cells of lactic acid bacteria could be an alternative or complementary method of immobilizing organic acids and bacteriocins and inhibit undesirable bacteria in foods. this study evaluated the inhibition potential of immobilized lactococcus lactis subsp. lactis atcc 11454 on selected bacteria by a modified method of the agar spot test. l. lactis was immobilized in calcium alginate (1 to 2%)-whey protein concentrate (0 and 1%) beads. the antimicrobial potential of immobil ... | 2004 | 15222547 |
| characterization of mesentericin st99, a bacteriocin produced by leuconostoc mesenteroides subsp. dextranicum st99 isolated from boza. | lactic acid bacteria isolated from boza, a cereal-fermented beverage from belogratchik, bulgaria, were screened for the production of bacteriocins. with the first screening, 13 of the 52 isolates inhibited the growth of listeria innocua and lactobacillus plantarum. the cell-free supernatant of one of these strains, classified as leuconostoc mesenteroides subsp. dextranicum st99, inhibited the growth of bacillus subtilis, enterococcus faecalis, several lactobacillus spp., lactococcus lactis subsp ... | 2004 | 15252717 |
| lactic acid bacteria as a potential source of enzymes for use in vinification. | 2004 | 15466506 | |
| detection and quantification of brettanomyces bruxellensis and 'ropy' pediococcus damnosus strains in wine by real-time polymerase chain reaction. | brettanomyces bruxellensis is a well-known wine spoilage yeast that causes undesirable off-flavours. likewise, glucan-producing strains of ropy pediococcus damnosus are considered as spoilage micro-organisms because the synthesis of glucan leads to an unacceptable viscosity of wine. | 2004 | 15479405 |
| isolation and characterization of anti-salmonella lactic acid bacteria from the porcine gastrointestinal tract. | to identify lactic acid bacteria (lab) of porcine intestinal origin with anti-salmonella activity. | 2004 | 15482434 |
| molecular characterization of lactic acid populations associated with wine spoilage. | we have investigated the prevalence of spoilage lactic acid bacteria (lab) in table wines produced in the apulia region. the occurrence of lab was evaluated in wines produced with low sulphur dioxide doses and not supplemented with selected malolactic starters such as oenococcus oeni. about 150 strains were isolated from wine must and a molecular characterization was performed using pcr-based techniques. most of the strains analysed belonged to lactobacillus plantarum species. however, some of t ... | 2004 | 14768022 |
| real-time pcr detection of lactic acid bacteria in cecal contents of eimeria tenella-lnfected broilers fed soybean oligosaccharides and soluble soybean polysaccharides. | this experiment was conducted to test whether dietary soybean meal oligosaccharides (smo) and water-soluble polysaccharides (smp) can assist broiler chickens in resisting eimeria tenella, and to determine the survival of lactic acid bacteria in cecal contents postinfection. all birds received a soybean meal-free diet. the 6 experimental treatments were as follows: positive (cor) and negative (cow) control groups, 2 groups fed diets containing either 1% smo or 0.5% smp from 1 to 11 d of age; a va ... | 2004 | 15510555 |
| the ph-unrelated influence of salt, temperature and manganese on aroma formation by staphylococcus xylosus and staphylococcus carnosus in a fermented meat model system. | the influence of manganese (0.01-0.1-1.0 microg/g), temperature (15-24 degrees c) and salt (3-4% w/w) on volatile formation in model minces inoculated with pediococcus pentosaceus and either staphylococcus xylosus or staphylococcus carnosus was studied in a full factorial experiment. in order to study the direct, ph-unrelated effect of the parameters, data were analysed by use of multiple linear regression and partial least-squares regression both before and after transformation of the volatile ... | 2004 | 15527916 |
| generation and utilization of polyclonal antibodies to a synthetic c-terminal amino acid fragment of divercin v41, a class iia bacteriocin. | polyclonal antibodies have been generated by immunization of rabbits with a chemically synthesized c-terminal part of divercin v41 (dvnct) conjugated to the carrier protein keyhole limpet hemocyanin (klh). the sensitivity and reactivity of the dvnct-klh-generated antibodies were evaluated by enzyme-linked immunosorbent assay (elisa) using supernatant from cultures of 13 representative lactic acid bacterium strains, and specificity was confirmed by western blot analysis. anti-dvnct-klh antibodies ... | 2004 | 14711648 |
| microbiological characterization and probiotic potential of koko and koko sour water, african spontaneously fermented millet porridge and drink. | to identify and examine the diversity of predominant lactic acid bacteria (lab) in koko and koko sour water (ksw) from different ghanaian production sites with regard to pattern of fermentation (api 50 chl), genotype, antimicrobial activity, and resistance to low ph and bile salts. | 2004 | 14723700 |
| observations on the succession dynamics of lactic acid bacteria populations in chill-stored vacuum-packaged beef. | drip samples were collected at 4-week intervals from 10 vacuum-packaged beef striploins stored for 16 weeks at -1.5 degrees c and assayed for populations of lactic-acid bacteria (lab), ph and spoilage-causing fermentation products. a total of 15 lab species were identified using pulsed-field gel electrophoresis and biochemical analysis. a pattern of succession was observed during storage between strains of carnobacterium, lactobacillus, leuconostoc and pediococcus. acetic acid production was ass ... | 2004 | 14751682 |
| a rapid turbidometric microplate bioassay for accurate quantification of lactic acid bacteria bacteriocins. | a 1 day turbidometric microplate bioassay (tmb) was developed for the rapid, accurate and precise quantification of lactic acid bacteria (lab) bacteriocins (nisin z and pediocin pa-1). parameters such as the concentration of the indicator strains and the incubation time were optimized for each bacteriocin. a high correlation coefficient (r(2)=0.992+/-0.004) was obtained for the exponential regression in the nisin z concentration range of 20-120 ng/ml with 1 x 10(7) cfu indicator strain (pediococ ... | 2004 | 14751683 |
| production of plantaricin nc8 by lactobacillus plantarum nc8 is induced in the presence of different types of gram-positive bacteria. | lactobacillus plantarum nc8 was shown to produce plantaricin nc8 (plnc8), a recently purified and genetically characterized inducible class iib bacteriocin, only when it was co-cultured with other gram-positive bacteria. among 82 strains belonging to the genera bacillus, enterococcus, lactobacillus, lactococcus, leuconostoc, listeria, pediococcus, staphylococcus, and streptococcus, 41 were shown to induce plnc8 production in l. plantarum nc8. there was apparently no relationship between the sens ... | 2004 | 14647979 |
| pediocin production by pediococcus acidilactici in solid state culture on a waste medium: process simulation and experimental results. | the production of pediocin by pediococcus acidilactici was comparatively studied in submerged and solid-state culture, using polyurethane foam particles soaked in commercial (mrs) and waste media with various supplements, where product concentrations were 15 times higher in mrs medium. for the solid state analysis, cultures were treated by successive compression and refilling of tubular minireactors equipped with a piston, without the need for reinoculation. this method was found to be simple, r ... | 2004 | 14966809 |
| relative ability of orally administered lactobacillus murinus to predominate and persist in the porcine gastrointestinal tract. | five porcine-derived lactobacillus or pediococcus isolates administered to pigs (n = 4), either singly or as a combination at approximately 10(10) cfu per day varied with respect to intestinal survival and persistence. two lactobacillus murinus strains survived best and were excreted at approximately 10(7) to 10(8) cfu/g of feces. in contrast, pediococcus pentosaceus dpc6006 had the lowest fecal count at approximately 10(5) cfu/g and was excreted at a significantly lower level than both l. murin ... | 2004 | 15066778 |
| a pcr assay for detection of acetic acid-tolerant lactic acid bacteria in acidic food products. | a pcr assay for the detection of acetic acid-tolerant lactic acid bacteria in the genera of lactobacillus and pediococcus was developed in this study. primers targeting the bacterial 16s rrna gene were newly designed and used in this pcr assay. to determine the specificity of the assay, 56 different bacterial strains (of 33 genera), 2 fungi, 3 animals, and 4 plants were tested. results were positive for most tested bacterial members of 16s rrna gene-based phylogenetic groups (classified in the l ... | 2004 | 15035383 |
| genotypic analyses of lactobacilli with a range of tannase activities isolated from human feces and fermented foods. | a total of 77 tannase producing lactobacilli strains isolated from human feces or fermented foods were examined for their genotypic profiles and intensities of tannase production. with a pcr-based assay targeting reca gene, all strains except one isolate were assigned to either lactobacillus plantarum, l. paraplantarum, or l. pentosus whereas a 16/23s rdna targeted pcr-based assay identified all except 6 isolates (inclusive of the above one isolate) as one of the closely related species. subsequ ... | 2004 | 15053327 |
| selective antimicrobial activity of chitosan on beer spoilage bacteria and brewing yeasts. | chitosan (0.1 g l(-1)), assayed in a simple medium, reduced the viability of four lactic acid bacteria isolated during the beer production process by 5 logarithmic cycles, whereas activity against seven commercial brewing yeasts required up to 1 g chitosan l(-1). antimicrobial activity was inversely affected by the ph of the assay medium. in brewery wort, chitosan (0.1 g l(-1)) selectively inhibited bacterial growth without altering yeast viability or fermenting performance. | 2004 | 15168856 |
| diversity of lactic acid bacteria isolated from aoc salers cheese. | the objective of this work was to describe the diversity of lactic acid bacteria in traditional raw milk salers cheeses at the species and strain levels. the characterization of 381 strains isolated during ripening and various strain collections was investigated using physiological analysis and molecular techniques: rep-pcr, species and genus specific amplifications and the sequence analysis of 16s rdna for strain typing and taxonomic identification. the strains belonged to lactobacillus plantar ... | 2004 | 15190953 |
| retrieving sequences of enzymes experimentally characterized but erroneously annotated : the case of the putrescine carbamoyltransferase. | annotating genomes remains an hazardous task. mistakes or gaps in such a complex process may occur when relevant knowledge is ignored, whether lost, forgotten or overlooked. this paper exemplifies an approach which could help to resuscitate such meaningful data. | 2004 | 15287962 |
| lactic acid bacteria used in inoculants for silage as probiotics for ruminants. | many studies have shown the beneficial effects on ruminant performance of feeding them with silages inoculated with lactic acid bacteria (lab). these benefits might derive from probiotic effects. the purpose of the current study was to determine whether lab included in inoculants for silage can survive in rumen fluid (rf), as the first step in studying their probiotic effects. experiments were conducted in the united states and israel with clarified (crf) and strained rf (srf) that were inoculat ... | 2004 | 15304734 |
| effects of indigenous starter cultures on the microbial and physicochemical characteristics of urutan, a balinese fermented sausage. | urutan is a balinese traditional dry fermented sausage prepared from lean pork and various kinds of spice. urutan is different from the european sausages, because it is fermented under warm condition with fluctuating temperatures of approximately 25 degrees c at night to 50 degrees c during sun drying. in this study, two of the 71 strains of lactic acid bacteria (lab) isolated from natural urutan fermentation were used as starter cultures: lactobacillus plantarum u201, the dominant lab, and pedi ... | 2004 | 16233672 |
| isolation, identification, and selection of lactic acid bacteria from alfalfa sprouts for competitive inhibition of foodborne pathogens. | several studies have investigated the control of pathogens on alfalfa sprouts, and some treatments have been shown to be effective in reducing pathogen populations. however, control methods investigated thus far only provide pathogen control at a given point in the sprouting process and can affect germination. competitive inhibition of pathogens with lactic acid bacteria might provide pathogen control throughout the sprouting process and up to consumption. the purpose of this study was to isolat ... | 2004 | 15151232 |
| purification and characterization of bacteriocin from pediococcus pentosaceus accel. | the pediococcus pentosaceus accel bacteriocin was purified to electrophoretical homogeneity by cell adsorption-desorption and superose 12 fast performance liquid chromatography (fplc). the purified bacteriocin, with a molecular mass of 17.5 kda and an n-terminal sequence of -kyygngvtxgkhsxxvdxg-, belongs to class iia and is designated pediocin accel. it was inactivated by various proteases and stable at ph 2.0-6.0 and <100 degrees c. more than 80% activity was left even after 15 min of heating a ... | 2004 | 14995112 |
| intra-specific variation of lactobacillus plantarum and lactobacillus pentosus in sensitivity towards various bacteriocins. | fifty-two strains belonging to the lactobacillus plantarum species group were identified and typed. they represented 32 clones of lactobacillus plantarum and 7 clones of lactobacillus pentosus. sensitivity of all strains towards bacteriocins of four different producer strains was investigated using a deferred inhibition test (dit). substantial intra-specific variation in sensitivity of clones was observed towards bacteriocinogenic lactic acid bacteria producing nisin ( lactococcus lactis atcc 11 ... | 2004 | 15028870 |
| cloning, deletion, and characterization of padr, the transcriptional repressor of the phenolic acid decarboxylase-encoding pada gene of lactobacillus plantarum. | lactobacillus plantarum displays a substrate-inducible pada gene encoding a phenolic acid decarboxylase enzyme (pada) that is considered a specific chemical stress response to the inducing substrate. the putative regulator of pada was located in the pada locus based on its 52% identity with padr, the pada gene transcriptional regulator of pediococcus pentosaceus (l. barthelmebs, b. lecomte, c. diviès, and j.-f. cavin, j. bacteriol. 182:6724-6731, 2000). deletion of the l. plantarum padr gene cle ... | 2004 | 15066807 |
| detection of resistance of lactic acid bacteria to a mixture of the hop analogue compounds tetrahydroiso-alpha-acids by noninvasive measurement of intracellular ph. | to examine the resistance of beer isolates of lactic acid bacteria (lab) towards a mixture of tetrahydroiso-alpha-acids (tetra) by growth experiments as well as by measurement of intracellular ph. | 2004 | 15139925 |
| isolation and partial characterization of bacteriocins from pediococcus species. | lactic acid bacteria have received increased attention as a potential food preservative due to their strong antagonistic activity against many food-spoilage and pathogenic organisms. three pediococcus species, p. acidilactici ncim 2292 , p. pentosaceous. ncim 2296 and p. cervisiae ncim 2171, were evaluated for bacteriocin production. inhibitory substance were produced during the late growth phase and maximum production occurred at 37 degrees after 36-48 h of incubation. bacteriocins partially pu ... | 2004 | 15205931 |
| comparative analysis of conserved genetic markers and adjacent dna regions identified in beer-spoilage lactic acid bacteria. | to conduct an inter-species comparative study on the nucleotide sequences of the conserved dna regions surrounding orf5, a genetic marker for differentiating beer-spoilage lactic acid bacteria. | 2004 | 15287868 |
| resistance screening essay of wine lactic acid bacteria on lysozyme: efficacy of lysozyme in unclarified grape musts. | in wine making, the bacteriolytic activity of lysozyme has primarily been used to control the malolactic fermentation in wines. the use of lysozyme in musts before settling and the beginning of the alcoholic fermentation to inhibit the growth of lactic acid bacteria could be very beneficial. in a resistance test carried out in mt/b broth, lysozyme had greater antimicrobial activity toward oenococcus oeni than lactobacillus species. several strains of wine bacteria belonging to oenococcus proved ... | 2004 | 15053521 |
| synthesis of an alpha-linked dimer of the trisaccharide repeating unit of the exopolysaccharide produced by pediococcus damnosus 2.6. | a hexasaccharide, beta-d-glcp-(1-->3)-[beta-d-glcp-(1-->2)]-alpha-d-glcp-(1-->3)-beta-d-glcp-(1-->3)-[beta-d-glcp-(1-->2)]-d-glcp, the alpha-linked dimer of the trisaccharide repeating unit of the exopolysaccharide produced by pediococcus damnosus 2.6, was synthesized as its methyl glycoside. condensation of fully benzoylated alpha-d-glucopyranosyl trichloroacetimidate (1) with isopropyl 4,6-o-benzylidene-1-thio-beta-d-glucopyranoside (2) selectively furnished (1-->3)-linked disaccharide 3, and ... | 2004 | 15476710 |
| population dynamics of the constitutive biota of french dry sausages in a pilot-scale ripening chamber. | the population dynamic of constitutive biota on 84 samples belonging to two different types of french fermented dry sausages during the ripening process in a pilot-scale ripening chamber was investigated. samples were analyzed in three steps of their production: fresh product, first drying stage, and finished product. in addition, 180 strains of lactic acid bacteria were identified using a miniaturized biochemical procedure of characterization. in general, the number of lactic acid bacteria that ... | 2004 | 15508649 |
| the microflora of fermented nixtamalized corn. | nixtamalization is a traditional process that improves the nutritional quality of corn. to provide a means of utilizing the nutritional benefits of nixtamalized corn and improve product acceptability, lactic acid fermentation was applied. the objective of the study was to study the microbial profile and establish the important lactobacilli of fermenting nixtamalized corn dough. two batches of cleaned whole corn were subjected to the process of nixtamalization, using two concentrations of lime (0 ... | 2004 | 15358510 |
| development of a fast dna-dna hybridization method based on melting profiles in microplates. | dna-dna hybridization is still the "gold standard" for the genotypic delineation of bacterial species. however, it is not widely used because traditional dna-dna hybridization techniques are rather time-consuming and not easy to perform in routine laboratories. in the present study, dna of reference strains was digested with sau3a, ligated with linker oligonucleotides s1/2 and in vitro amplified. the amplified dna fragments were immobilized on maxisorb 96-well plates. dna isolated from target st ... | 2004 | 15612626 |
| peptones from autohydrolysed fish viscera for nisin and pediocin production. | various peptones obtained from hydrolysed visceral homogenates of four fishery residues showed their suitability for promoting the growth of lactic acid bacteria, micro-organisms with particularly complex requirements regarding peptidic nutrients. the assay of several treatments with two bacterial species, producers of the two main bacteriocins (nisin and pediocin) demostrated that optimum conditions only imply a brief autohydrolysis at natural ph and room temperature, with subsequent steam-flow ... | 2004 | 15313007 |
| comparative composition of bacteria in the human intestinal microflora during remission and active ulcerative colitis. | ulcerative colitis is a severe, relapsing and remitting disease of the human large intestine characterised by inflammation of the mucosa and submucosa. the main site of disease is the sigmoid/rectal region of the large bowel but the aetiology remains unknown. there is considerable evidence to indicate that the components of the resident colonic microflora can play an important role in initiation of the disease. the present study was aimed at characterising the faecal microflora of ulcerative col ... | 2004 | 15460067 |
| fluorescent in situ hybridization analysis of open lactic acid fermentation of kitchen refuse using rrna-targeted oligonucleotide probes. | reproducible amounts of lactic acid accumulate in minced kitchen refuse under open conditions with intermittent ph neutralization [sakai et al., food sci. technol. res., 6, 140 (2000)]. here, we showed that such ph-controlled open fermentation of kitchen refuse reproducibly resulted a selective proliferation of a major lactic acid bacterial (lab) species. in one experiment, the predominant microorganisms isolated during the early phase (6 h) were gammaproteobacteria. in contrast, those that pred ... | 2004 | 16233665 |
| temporal stability analysis of the microbiota in human feces by denaturing gradient gel electrophoresis using universal and group-specific 16s rrna gene primers. | according to the current insights, the predominant bacterial community in human feces is considered to be stable and unique for each individual over a prolonged period of time. in this study, the temporal stability of both the predominant population and a number of specific subpopulations of the fecal microbiota of four healthy volunteers was monitored for 6-12 weeks. for this purpose, a combination of different universal (v(3) and v(6)-v(8)) and genus- or group-specific (targeting the bacteroid ... | 2004 | 19712312 |
| volatiles in a sausage surface model-influence of penicillium nalgiovense, pediococcus pentosaceus, ascorbate, nitrate and temperature. | thirty-two agar sausage models were arranged in a 2((5-1)) fractional factorial design to analyse the effects of penicillium nalgiovense growth, pediococcus pentosaceus starter, sodium ascorbate, sodium nitrate and temperature on 79 volatiles produced during incubation. the model focused on the outer 10 millimeters of sausages. ascorbate addition showed clear antioxidative effects, and reduced the amount of more than half of all volatiles but increased concentrations of 2-methyl-propanal and 3-m ... | 2004 | 22064148 |
| growth and production of volatiles by staphylococcus carnosus in dry sausages: influence of inoculation level and ripening time. | three sausage batches inoculated with normal inoculation level of pediococcus pentosaceus (5×10(6) cfu/g) and with low, intermediate, and high inoculation levels of staphylococcus carnosus (10(5), 5×10(6), 5×10(7) cfu/g, respectively) were produced. cell counts and formation of volatiles were followed throughout a ripening period of three weeks. the staphylococci exhibited the fastest growth in sausages with a low inoculation level, whereas growth was only moderate in sausages with a high initia ... | 2004 | 22061519 |
| effect of starter culture, spice mix and storage time and temperature on biogenic amine content of dry fermented sausages. | two types of dry fermented sausage differing in spicing mixture and the diameter (low content of red pepper+diameter 80 mm, h-sausage; high content of red pepper+diameter 55 mm, p-sausage, respectively) were produced in parallel with two different starter cultures (pediococcus pentosaceus+staphylococcus carnosus, b-samples and s. carnosus+staphylococcus xylosus+lactobacillus farciminis, f-samples, respectively). the sausages were ripened 21 days and subsequently stored 91 days at the room temper ... | 2004 | 22061810 |
| addition of a-ketoglutarate enhances formation of volatiles by staphylococcus carnosus during sausage fermentation. | the effect of leucine and a-ketoglutarate addition on transamination of branched-chain amino acids was studied in model minces inoculated with pediococcus pentosaceus and staphylococcus carnosus. leucine addition changed the ratio of volatile breakdown products of leucine, isoleucine and valine but not the total amount of the volatiles and it was concluded that the amount of free amino acids does not limit transamination of amino acids. the addition of a-ketoglutarate resulted in increased level ... | 2004 | 22061822 |
| a fermented meat model system for studies of microbial aroma formation. | a fermented meat model system was developed, by which microbial formation of volatiles could be examined. the model was evaluated against dry, fermented sausages with respect to microbial growth, ph and volatile profiles. fast and slowly acidified sausages and models were produced using the starter cultures pediococcus pentosaceus and staphylococcus xylosus. volatiles were collected and analysed by dynamic headspace sampling and gc-ms. the analysis was primarily focused on volatiles arising from ... | 2004 | 22063950 |
| characterization of lactic acid bacteria isolated from a greek dry-fermented sausage in respect of their technological and probiotic properties. | a total of 147 lactic acid bacteria was isolated from two types of naturally fermented dry sausages at four different stages of the ripening process studied in order to select the most suitable strains according to their technological characteristics including probiotic properties and antimicrobial activity against food-borne pathogens. identification of the isolates revealed that 90% were lactobacilli, 4% enterococci, 3% pediococcus sp. and sporadic isolates of weissella viridescens, leuconosto ... | 2003 | 22063449 |
| beer spoilage bacteria and hop resistance. | for brewing industry, beer spoilage bacteria have been problematic for centuries. they include some lactic acid bacteria such as lactobacillus brevis, lactobacillus lindneri and pediococcus damnosus, and some gram-negative bacteria such as pectinatus cerevisiiphilus, pectinatus frisingensis and megasphaera cerevisiae. they can spoil beer by turbidity, acidity and the production of unfavorable smell such as diacetyl or hydrogen sulfide. for the microbiological control, many advanced biotechnologi ... | 2003 | 14623377 |
| 16s-ardra, a tool for identification of lactic acid bacteria isolated from grape must and wine. | lactic acid bacteria (lab) are found in a great variety of habitats, including grape must and wines. there is a close relationship between the species of lab which develop during fermentation and the eventual quality of the wine. for these reasons analytical techniques allowing fast and reliable identification of wine lab are needed. in this work a simple and accurate protocol for identifying species of lab isolated from grape must and wine is presented. this protocol is based on the amplificati ... | 2003 | 14529184 |
| islpl1 is a functional is30-related insertion element in lactobacillus plantarum that is also found in other lactic acid bacteria. | we describe the first functional insertion sequence (is) element in lactobacillus plantarum. islpl1, an is30-related element, was found on the plp3 plasmid in strain fb335. by selection of spontaneous mutants able to grow in the presence of uracil, it was demonstrated that the is had transposed into the uracil phosphoribosyltransferase-encoding gene upp on the fb335 chromosome. the plasmid-carried is element was also sequenced, and a second potential is element was found: islpl2, an is150-relate ... | 2003 | 14532059 |
| development of specific fluorescent oligonucleotide probes for in situ identification of wine lactic acid bacteria. | a rapid method for the identification of lactic acid bacteria (lab) from wine has been developed. this method is based on fluorescence in situ hybridisation (fish), using fluorescent oligonucleotide probes, homologous to 16s rdna of those species of lab commonly found in wines. the protocol for the specific detection of these bacteria was established through the hybridisation of 36 reference strains. the specificity of the probes was evaluated by using pure cultures. probes were used to identify ... | 2003 | 12900029 |
| exopolysaccharide production by pediococcus damnosus 2.6 in a semidefined medium under different growth conditions. | ropy pediococcus damnosus (strain 2.6) was used for production of exopolysaccharide (eps) in a semidefined medium. from a kinetic point of view, an experiment conducted in smd medium containing 30 g l(-1) glucose and 5 g l(-1) bacto casamino acids (difco laboratories, detroit, mi), without ph control, showed that eps production took place mainly during the growth phase. the viscosity of the cultures developed in parallel to the eps synthesis until 94 h of incubation; after 200 h of fermentation, ... | 2003 | 12927713 |
| functional replacement of the escherichia coli d-(-)-lactate dehydrogenase gene (ldha) with the l-(+)-lactate dehydrogenase gene (ldhl) from pediococcus acidilactici. | the microbial production of l-(+)-lactic acid is rapidly expanding to allow increased production of polylactic acid (pla), a renewable, biodegradable plastic. the physical properties of pla can be tailored for specific applications by controlling the ratio of l-(+) and d-(-) isomers. for most uses of pla, the l-(+) isomer is more abundant. as an approach to reduce costs associated with biocatalysis (complex nutrients, antibiotics, aeration, product purification, and waste disposal), a recombinan ... | 2003 | 12676706 |
| bioprotectives and probiotics for dry sausages. | the microbial stability of dry sausages is determined by the combination and timing of different factors referred to as the hurdle-concept. however, the hurdles present in dry sausage are not sufficient to prevent the survival of listeria monocytogenes or enterohemorrhagic escherichia coli o157:h7. recently bioprotective lactic acid bacteria, which in addition to the production of antimicrobial lactic acid, have been found to contribute to the safety of the dry sausage by producing antimicrobial ... | 2003 | 12745229 |
| survival of lactic acid bacteria in seawater: a factorial study. | a feasibility study of lactic bacteria as potential probiotics in larval cultures of marine fish was performed by investigating the survival of five strains of lactic bacteria in seawater by readily standardized procedures at different temperatures and salinities. these conditions were chosen in such a way that their combinations define a complete first-order factorial design. depending on the strain and the ambient conditions, the survival adhered to first-order kinetics in some cases, and to t ... | 2003 | 14756536 |
| bacteriocins from pediococcus pentosaceus l and s from pork meat. | two strains of pediococcus pentosaceus were isolated from refrigerated pork and found to produce antimicrobial substances that may inhibit foodborne pathogens and have potential as natural food preservatives. they were named p. pentosaceus l and s. the antimicrobial substances were purified to electrophoretical homogeneity by chloroform extraction and designated pentocins l and s with molecular masses (m) of 27 and 25 kda, respectively. both pentocins also had broad inhibition spectra and were t ... | 2003 | 12568574 |
| identification, isolation and quantification of representative bacteria from fermented cassava dough using an integrated approach of culture-dependent and culture-independent methods. | the use of denaturing gradient gel electrophoresis (dgge) and traditional culture-depending methods for examining the bacterial community of traditional cassava starch fermentation were investigated. it appeared that dgge profiles of total dna of cassava dough exhibited 10 distinguishable bands. in contrast, dgge fingerprints of bacteria recovered from enrichment cultures of fermented dough gave variable profiles containing fewer bands. bands corresponding to five bacterial species detected by d ... | 2003 | 12568751 |
| strong synergy between a eukaryotic antimicrobial peptide and bacteriocins from lactic acid bacteria. | the antimicrobial effect obtained upon combining the prokaryotic antimicrobial peptides (amps; more commonly referred to as bacteriocins) pediocin pa-1, sakacin p, and curvacin a (all produced by lactic acid bacteria [lab]) with the eukaryotic amp pleurocidin (from fish) has been investigated. the three lab amps alone were active against gram-positive listeria ivanovii bacteria at nanomolar concentrations, whereas they were inactive against gram-negative escherichia coli bacteria. pleurocidin al ... | 2003 | 12620872 |
| evaluation of several modifications of an ecometric technique for assessment of media performance. | recovery of listeria monocytogenes 101m, jonesia denitrificans, salmonellae, and pediococcus sp. nrrl b-2354 across nine media was evaluated with three modified versions of an ecometric method. two approaches involved the use of broth cultures (10(8) to 10(9) cfu/ml) of individual strains and either large (10-microl) or small (1-microl) presterilized plastic loops. the third approach involved precultured slants and the inoculation of media with presterilized plastic inoculating needles (10(4) cf ... | 2003 | 14503736 |
| enumeration of probiotic pediococci in animal feed: interlaboratory study. | an enumeration method to be used as official control under council directive 70/524/eec for probiotic pediococci used as feed additives was validated for consideration for adoption as comitée européen de normalisation (cen) and iso standards. seventeen laboratories in 11 european countries carried out an interlaboratory study. a spread plate method following bs iso 15214:1998 using 4 different agars [mrs, acidified mrs, mrs with triphenyl tetrazolium chloride (ttc), and a newly developed pedioco ... | 2003 | 14509440 |
| characterization and heterologous expression of a class iia bacteriocin, plantaricin 423 from lactobacillus plantarum 423, in saccharomyces cerevisiae. | lactobacillus plantarum 423 produces a small heat-stable antimicrobial protein designated plantaricin 423. this protein is bactericidal for many gram-positive foodborne pathogens and spoilage bacteria, including listeria spp., staphylococcus spp., pediococcus spp., lactobacillus spp., etc. the dna sequence of the plantaricin 423-encoding region on plasmid ppla4 revealed a four open reading frame (orf) operon structure similar to pediocin pa-1/ach from pediococcus acidilactici and coagulin from b ... | 2003 | 12423916 |
| conjugated linoleic acid biosynthesis by human-derived bifidobacterium species. | to assess strains of lactobacillus, lactococcus, pediococcus and bifidobacterium for their ability to produce the health-promoting fatty acid conjugated linoleic acid (cla) from free linoleic acid. | 2003 | 12492934 |
| construction and use of a computerized dna fingerprint database for lactic acid bacteria from silage. | efficient selection of new silage inoculant strains from a collection of over 10,000 isolates of lactic acid bacteria (lab) requires excellent strain discrimination. toward that end, we constructed a gelcompar ii database of dna fingerprint patterns of ethidium bromide-stained ecori fragments of total lab dna separated by conventional agarose gel electrophoresis. we found that the total dna patterns were strain-specific; 56/60 american type culture collection strains of 33 species of lab could b ... | 2003 | 14607400 |
| the effects of using lactic acid bacteria inoculants in maize silage on the formation of biogenic amines. | silages from five ripened varieties of silage maize with dry matter contents ranging between 275 and 410 g/kg were prepared in five laboratory experiments. whole-plant maize was fermented at 22 degrees c and silages were then stored at the same temperature for 4 months. spontaneously fermented silages were prepared as control variants and compared with silages inoculated with commercial strains of lactobacillus plantarum, lactobzcillus buchneri and a mixed preparation microsil containing l. plan ... | 2003 | 14620909 |
| thermal inactivation of pediococcus sp. in simulated apple cider during high-temperature short-time pasteurization. | prompted by concerns regarding outbreaks of food-borne illness which have occurred due to the consumption of commercial, nonpasteurized fruit juices contaminated with escherichia coli o157:h7, the us food and drug administration and canadian food inspection agency are considering several new safety standards to apply to fresh juices, including mandatory pasteurization of all apple cider. in support of these initiatives, a study was conducted to evaluate the pasteurization of simulated cider usin ... | 2003 | 12505457 |
| substrate inhibition of pediococcus acidilactici by glucose on a waste medium. simulations and experimental results. | the possibility of substrate inhibition by glucose on biomass and pediocin production was studied in cultures of pediococcus acidilactici on a residual medium. | 2003 | 14633105 |
| use of starter cultures of lactic acid bacteria and yeasts in the preparation of togwa, a tanzanian fermented food. | starter cultures of lactic acid bacteria (lactobacillus brevis, lactobacillus cellobiosus, lactobacillus fermentum, lactobacillus plantarum and pediococcus pentosaceus) and yeasts (candida pelliculosa, candida tropicalis, issatchenkia orientalis and saccharomyes cerevisiae) isolated from native togwa were tested singly or in combination for their ability to ferment maize-sorghum gruel to produce togwa. all species of bacteria showed an ability to ferment the gruel as judged by lowering the ph fr ... | 2003 | 12745235 |
| proteolytic activities in togwa, a tanzanian fermented food. | proteolytic activities were investigated in sorghum-based togwa prepared by natural fermentation and using starter cultures previously isolated from the native product, i.e., lactobacillus brevis, lactobacillus cellobiosus, lactobacillus fermentum, lactobacillus plantarum, pediococcus pentosaceus, and issatchenkia orientalis in coculture with either l. brevis or l. plantarum. both proteinase and aminopeptidase activities were substantially higher in naturally fermented togwa than in those with s ... | 2003 | 12781948 |
| potential osmoprotectants for the lactic acid bacteria pediococcus pentosaceus and tetragenococcus halophila. | the physiological responses of the lactic acid bacteria pediococcus pentosaceus and tetragenococcus halophila (formely known as p. halophila), subjected to osmotic stress in the presence of molecules known to act as osmoprotectants for other bacteria were studied. in a defined medium, glycine betaine, dimethylsulfonioacetate, choline, proline and l-carnitine were able to relieve inhibition of growth at 0.8 m nacl. the five compounds were shown to efficiently compete with glycine betaine transpor ... | 2003 | 12781949 |
| identification of carnobacterium, lactobacillus, leuconostoc and pediococcus by rdna-based techniques. | ribosomal dna-based techniques including the analysis of profiles generated by isr amplification, isr restriction and ardra have been evaluated as molecular tools for identifying carnobacterium, lactobacillus, leuconostoc and pediococcus. they have been applied for the molecular characterization of 91 strains with the following identities: eight carnobacterium including the eight type species of the genus; 61 lactobacillus including 40 type strains out of 45 species, 13 leuconostoc, out of them ... | 2003 | 14666983 |
| two distinct pathways for the formation of hydroxy fa from linoleic acid by lactic acid bacteria. | twenty-three of 86 strains of lactic acid bacteria transformed linoleic acid into hydroxy fa. two distinct conversion pathways were in operation. two strains of lactobacillus acidophilus and a strain of pediococcus pentosaceus produced 13(s)-hydroxy-9-octadecenoic acid 113(s)-oh 18:11 and 10,13dihydroxyoctadecanoic acid (10,13-oh 18:0) as main and minor products, respectively, whereas 13 strains, including l. casei subsp. casei, l. paracasei subsp. paracasei, l. rhamnosus, l. lactis subsp. cremo ... | 2003 | 14870930 |
| cloning, sequence analysis and expression of the f1f0-atpase beta-subunit from wine lactic acid bacteria. | the nucleotide sequences of the genes encoding the f1f0-atpase beta-subunit from oenococcus oeni, leuconostoc mesenteroides subsp. mesenteroides, pediococcus damnosus, pediococcus parvulus, lactobacillus brevis and lactobacillus hilgardii were determined. their deduced amino acid sequences showed homology values of 79-98%. data from the alignment and atpase tree indicated that o. oeni and l. mesenteroides subsp. mesenteroides formed a group well-separated from p. damnosus and p. parvulus and fro ... | 2003 | 14529177 |
| autolytic activity and pediocin-induced lysis in pediococcus acidilactici and pediococcus pentosaceus strains. | to evaluate the autolytic phenotype of pediococcus acidilactici and p. pentosaceus, the peptidoglycan hydrolases content and the effect of pediocin ach/pa-1 and autolysins on cell lysis. | 2003 | 12631191 |
| isolation, selection, and characterization of lactic acid bacteria for a competitive exclusion product to reduce shedding of escherichia coli o157:h7 in cattle. | lactic acid bacteria (lab) were selected on the basis of characteristics indicating that they would be good candidates for a competitive exclusion product (cep) that would inhibit escherichia coli o157:h7 in the intestinal tract of live cattle. fecal samples from cattle that were culture negative for e. coli o157:h7 were collected. lab were isolated from cattle feces by repeated plating on deman rogosa sharpe agar and lactobacillus selection agar. six hundred eighty-six pure colonies were isolat ... | 2003 | 12636285 |
| lactobacillus thermotolerans sp. nov., a novel thermotolerant species isolated from chicken faeces. | five strains of thermotolerant lactic acid bacteria (g 12, g 22, g 35t, g 43 and g 44) isolated from chicken faeces were characterized taxonomically. the strains were facultatively anaerobic, gram-positive, catalase-negative, non-motile, non-spore-forming rods. they were heterofermentative lactobacilli that produced dl-lactic acid. growth of the strains occurred at 45 degrees c but not at 15 degrees c. the optimum temperature for growth was 42 degrees c, as determined from the specific growth ra ... | 2003 | 12656183 |
| bacteriological evaluation of dog and cat diets that claim to contain probiotics. | nineteen commercial pet foods claiming to contain probiotics were evaluated. selective bacterial culture was performed to identify organisms that were claimed to be present. twelve diets claimed only to contain specific bacterial fermentation products, which does not necessarily indicate that live growth would be expected, but these products also included the term "probiotic" somewhere on the package, suggesting that live, beneficial organisms were present. no products contained all of the liste ... | 2003 | 12677689 |
| inhibition of nonproteolytic clostridium botulinum with lactic acid bacteria and their bacteriocins at refrigeration temperatures. | nonproteolytic clostridium botulinum (strains 17b, beluga, and 202f) was found to be inhibited by lactobacillus, lactococcus, streptococcus, and pediococcus species in tests by the spot-on-the-lawn simultaneous-antagonism method at 10, 15, and 25 degrees c. c. botulinum 17b was the most resistant strain. inhibition zone size increased with decreasing incubation temperature. six strains of lactobacillus acidophilus and seven strains of bifidobacteria failed to produce an inhibition zone on buffer ... | 2003 | 12696695 |
| conformational changes of pediocin in an aqueous medium monitored by fourier transform infrared spectroscopy: a biological implication. | fourier transform infrared (ftir) spectroscopy was used to investigate the secondary structure of pediocin pa-1 in different aqueous media in relation to its antimicrobial activity. the experiments were performed at pd (ph meter corrected for deuterium isotope effect) 6, 7, and 8 and during a heating-cooling cycle of 20-80 degrees c. at pd 6, (i.e. pediocin's most active form), the ftir results show that pediocin adopts an unordered structure with a small contribution of beta-turn. after a heati ... | 2003 | 12719125 |
| concomitant septic and gouty arthritis--an analysis of 30 cases. | to analyse the clinical features and outcomes of gouty patients with concomitant septic arthritis in a medical centre. | 2003 | 12730521 |
| influence of ph drop on both nisin and pediocin production by lactococcus lactis and pediococcus acidilactici. | to develop a kinetic model for describing the specific effect of ph drop on nisin and pediocin production in whey. | 2003 | 12803556 |
| prevalence and diversity of tetracycline resistant lactic acid bacteria and their tet genes along the process line of fermented dry sausages. | in order to study the prevalence and diversity of tetracycline resistant lactic acid bacteria (tc(r) lab) along the process line of two different fermented dry sausage (fds) types, samples from the raw meat, the meat batter and the fermented end product were analysed quantitatively and qualitatively by using a culture-dependent approach. both the diversity of the tet genes and their bacterial hosts in the different stages of fds production were determined. quantitative analysis showed that all r ... | 2003 | 12866855 |
| pediocin production by recombinant lactic acid bacteria. | production of the anti-listerial bacteriocin, pediocin, by lactic acid bacteria (lab) transformed with the cloning vector ppc418 (ped+, 9.1 kb) was influenced by composition of media and incubation temperature. maximum pediocin production, tested against listeria innocua, by electrotransformants of lactococcus lactis ssp. lactis was measured in tryptone/lactose/yeast extract medium after 24 h growth at 30 degrees c, while incubation at 40 degrees c was optimum for ped+ transformants of streptoco ... | 2003 | 12882274 |
| broad and complex antifungal activity among environmental isolates of lactic acid bacteria. | more than 1200 isolates of lactic acid bacteria isolated from different environments were screened for antifungal activity in a dual-culture agar plate assay. approximately 10% of the isolates showed inhibitory activity and 4% showed strong activity against the indicator mould aspergillus fumigatus. the antifungal spectra for 37 isolates with strong activity and five isolates with low or no activity were determined. several of the strains showed strong inhibitory activity against the moulds a. f ... | 2003 | 12594034 |
| macrolide and lincosamide resistance in the gram-positive nasal and tonsillar flora of pigs. | macrolide and lincosamide resistance phenotypes and the presence of the erm(a), erm(b), erm(c), and mef(a) genes were determined in 344 bacterial strains belonging to 34 species and nine genera, isolated from the tonsils and nasal cavities of 2-week- and 6-week-old piglets, derived from four different farms. these piglets had never before been treated with macrolides or lincosamides. macrolide and lincosamide resistance was most frequently present in streptococcus and enterococcus strains, of wh ... | 2003 | 12959408 |
| microbiological and fermentation characteristics of togwa, a tanzanian fermented food. | selected microbiological and metabolic characteristics of sorghum, maize, millet and maize-sorghum togwa were investigated during natural fermentation for 24 h. the process was predominated by lactic acid bacteria (lab) and yeasts. the mesophiles, lactic acid bacteria, and yeasts increased and the enterobacteriaceae decreased to undetectable levels within 24 h. the isolated microorganisms were tentatively identified as lactobacillus plantarum, lactobacillus brevis, lactobacillus fermentum, lacto ... | 2003 | 12423921 |
| purification and genetic characterization of plantaricin nc8, a novel coculture-inducible two-peptide bacteriocin from lactobacillus plantarum nc8. | a new, coculture-inducible two-peptide bacteriocin named plantaricin nc8 (plnc8) was isolated from lactobacillus plantarum nc8 cultures which had been induced with lactococcus lactis mg1363 or pediococcus pentosaceus fbb63. this bacteriocin consists of two distinct peptides, named alpha and beta, which were separated by c(2)-c(18) reverse-phase chromatography and whose complementary action is necessary for full plantaricin nc8 activity. n-terminal sequencing of both purified peptides showed 28 a ... | 2003 | 12514019 |
| phylogenetic diversity of lactic acid bacteria associated with paddy rice silage as determined by 16s ribosomal dna analysis. | a total of 161 low-g+c-content gram-positive bacteria isolated from whole-crop paddy rice silage were classified and subjected to phenotypic and genetic analyses. based on morphological and biochemical characters, these presumptive lactic acid bacterium (lab) isolates were divided into 10 groups that included members of the genera enterococcus, lactobacillus, lactococcus, leuconostoc, pediococcus, and weissella: analysis of the 16s ribosomal dna (rdna) was used to confirm the presence of the pre ... | 2003 | 12514026 |
| the lactic acid bacteria: a literature survey. | the purpose of this review article on the lactic acid bacteria grew from an early curiosity and a desire to convey and impart the broad scope of literary information on their functions as starter cultures, in the manufacture of fermentation products such as dairy products and alcoholic beverages, as well as their contribution to better health. this review article is an attempt to empower the reader and to circumvent the difficult task in acquiring and elucidating a large body of information. the ... | 2002 | 12546196 |
| novel food-grade plasmid vector based on melibiose fermentation for the genetic engineering of lactococcus lactis. | the alpha-galactosidase gene (aga) and a gene coding for a putative transcriptional regulator from the laci/galr family (galr) of lactococcus raffinolactis atcc 43920 were cloned and sequenced. when transferred into lactococcus lactis and pediococcus acidilactici strains, aga modified the sugar fermentation profile of the strains from melibiose negative (mel(-)) to melibiose positive (mel(+)). analysis of gala mutants of l. lactis subsp. cremoris mg1363 indicated that the putative galactose perm ... | 2002 | 12450840 |
| persistence of escherichia coli o157:h7 in barley silage: effect of a bacterial inoculant. | the effect of a lactic acid producing bacterial (lab) inoculant on the elimination of escherichia coli o157:h7 from barley forage was assessed. | 2002 | 12147077 |
| identification of the bacterial microflora in dairy products by temporal temperature gradient gel electrophoresis. | numerous microorganisms, including bacteria, yeasts, and molds, are present in cheeses, forming a complex ecosystem. among these organisms, bacteria are responsible for most of the physicochemical and aromatic transformations that are intrinsic to the cheesemaking process. identification of the bacteria that constitute the cheese ecosystem is essential for understanding their individual contributions to cheese production. we used temporal temperature gradient gel electrophoresis (ttge) to identi ... | 2002 | 12147461 |