Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| transmission of periodontopathic bacteria from natural teeth to implants. | purpose: prevention of peri-implantitis is essential for the success of implant rehabilitation. infection by periodontopathic bacteria is a major cause of peri-implantitis. the aim of the present study was to identify the source of peri-implant colonization by periodontopathic bacteria. materials and methods: twenty-one patients with implants were enrolled in the study. subgingival plaque samples from the adjacent, occluding, and contralateral natural teeth were collected prior to second-stage s ... | 2009 | 20002682 |
| defining the healthy "core microbiome" of oral microbial communities. | most studies examining the commensal human oral microbiome are focused on disease or are limited in methodology. in order to diagnose and treat diseases at an early and reversible stage an in-depth definition of health is indispensible. the aim of this study therefore was to define the healthy oral microbiome using recent advances in sequencing technology (454 pyrosequencing). | 2009 | 20003481 |
| microbiological status of periodontal diseases in lagos, nigeria. | the dynamic nature of oro-facial infections made it imperative to study the epidemiology of gingivitis and periodontitis which are significant clinical conditions in the nigerian environment. | 2009 | 20099785 |
| more than just drainage. | 2009 | 20830965 | |
| vaccine preparation by radiation processing. | a new radiation biotechnology for the acquirement of a commercial vaccine, designed for prophylaxis of ruminant infectious pododermatitis (ip), produced by gram negative bacteria fusobacterium necrophorum (f.n.), is presented. two different processes for preparing f.n. vaccine are used: a) the inactivation of f.n. bacteria exotoxins by microwave (mw) or/and electron beams (eb) irradiation; b) the isolation of exotoxins from f.n. cultures irradiated with mw or/and eb and the inactivation of isola ... | 2009 | 21384715 |
| factors contributing to the spread of odontogenic infections: a prospective pilot study. | spreading odontogenic infections (soi) are the commonest type of serious infections encountered in the orofacial region. a prospective multi-centre study was conducted in the west of scotland to investigate the contributing role of social, systemic and microbial factors in the pathogenesis of soi. | 2009 | 21509313 |
| bacterial diversity in persistent periapical lesions on root-filled teeth. | the purpose of this study was to analyze the bacterial diversity in persistent apical lesions on root-filled teeth by using culture-independent molecular methods. | 2009 | 21523213 |
| two-year outcome with nobel direct implants: a retrospective radiographic and microbiologic study in 10 patients. | the nobel direct implant (nobel biocare ab, göteborg, sweden) was developed to minimize marginal bone resorption and to result in "soft tissue integration" for an optimized aesthetic outcome. however, conflicting results have been presented in the literature. the aim of this present study was to evaluate the clinical and microbiologic outcomes of nobel direct implants. | 2009 | 18783421 |
| in vitro bactericidal activity of recombinant human beta-defensin-3 against pathogenic bacterial strains in human tooth root canal. | human beta-defensin-3 (hbd3), an endogenous antimicrobial peptide, has strong broad-spectrum antimicrobial activity. this study aimed to obtain recombinant hbd3 (rhbd3) and to test the hypothesis that the antimicrobial characteristics of hbd3 may offer an advantage over conventional medicine in reducing intracanal bacteria. genetic engineering was used to obtain active rhbd3 and analysis revealed that it exhibited a broad spectrum of antibacterial activity at low micromolar concentrations agains ... | 2009 | 18775647 |
| identification of a leukotoxin sequence from fusobacterium equinum. | 2009 | 18783899 | |
| an overview of the microbiology of acute ear, nose and throat infections requiring hospitalisation. | this study is the first to provide an extensive overview of the microbiology of acute ear, nose and throat infections requiring hospitalisation. all 2,028 cases of acute infections admitted between 1 january 2001 and 31 december 2006 were reviewed to assess the use of pre-admission antibiotics, microbiological results, antibiotic and surgical management and length of hospitalisation. infections of the oropharynx accounted for the vast majority of admissions, followed by ear infections, and cutan ... | 2009 | 18830726 |
| granulocyte chemotactic protein 2 (gcp-2/cxcl6) complements interleukin-8 in periodontal disease. | mucosal inflammatory responses are orchestrated largely by pro-inflammatory chemokines. the chemokine granulocyte chemotactic protein 2 (cxcl6) is involved in neutrophil recruitment and migration. previous studies have shown that granulocyte chemotactic protein 2 is up-regulated during mucosal inflammation (e.g. in inflammatory bowel disease), similarly to the functionally and structurally related chemokine interleukin-8. nevertheless, unlike interleukin-8, a role of granulocyte chemotactic prot ... | 2009 | 18842116 |
| biomaterial and antibiotic strategies for peri-implantitis: a review. | dental implants have 89% plus survival rates at 10-15 years, but peri-implantitis or dental implant infections may be as high as 14%. peri-implantitis can limit clinical success and impose health and financial burdens to patients and health providers. the pathogenic species associated with periodontitis (e.g., fusobacterium ssp, a. actinomycetemcomitans, p. gingivalis) are also associated with peri-implantitis. incidence of peri-implantitis is highest within the first 12 months after implantatio ... | 2009 | 18698626 |
| pericarditis caused by anaerobic bacteria. | this review describes the microbiology, diagnosis and management of pericarditis due to anaerobic bacteria. the predominant anaerobes isolated from patients with pericarditis are gram-negative bacilli (mostly bacteroides fragilis group) as well as peptostreptococcus, clostridium, fusobacterium, bifidobacterium and actinomyces spp. anaerobic bacteria can be recovered from pericarditis resulting from the following mechanisms: (i) spread from a contiguous site of infection, either de novo or follow ... | 2009 | 18789852 |
| the fusobacterium nucleatum outer membrane protein radd is an arginine-inhibitable adhesin required for inter-species adherence and the structured architecture of multispecies biofilm. | a defining characteristic of the suspected periodontal pathogen fusobacterium nucleatum is its ability to adhere to a plethora of oral bacteria. this distinguishing feature is suggested to play an important role in oral biofilm formation and pathogenesis, with fusobacteria proposed to serve as central 'bridging organisms' in the architecture of the oral biofilm bringing together species which would not interact otherwise. previous studies indicate that these bacterial interactions are mediated b ... | 2009 | 19007407 |
| photodynamic therapy as adjunct to non-surgical periodontal treatment in patients on periodontal maintenance: a randomized controlled clinical trial. | recent preclinical and clinical data have suggested the potential benefit of photodynamic therapy (pdt) in the treatment of periodontitis. however, currently, there are very limited data from controlled clinical trials evaluating the effect of pdt in the treatment of periodontitis. the aim of the present study was to evaluate the clinical and microbiological effects of the adjunctive use of pdt in non-surgical periodontal treatment in patients receiving supportive periodontal therapy. twenty-fou ... | 2009 | 18465191 |
| fusobacterium necrophorum: a ruminal bacterium that invades liver to cause abscesses in cattle. | fusobacterium necrophorum, a gram-negative, rod-shaped, and an aerotolerant anaerobe, is a normal inhabitant of the rumen of cattle. the organism is in ruminal contents and adherent to the ruminal wall. its role in ruminal fermentation is to metabolize lactic acid and degrade feed and epithelial proteins. the ruminal concentration is higher in grain-fed than forage-fed cattle. from the rumen, the organism gains entry into the portal circulation and is trapped in the liver to cause abscesses. the ... | 2009 | 18595747 |
| orbital cellulitis and cavernous sinus thrombosis secondary to necrobacillosis. | 2009 | 18600243 | |
| bacterial colonization of oral implants from nondental sources. | implants showing signs of peri-implantitis harbor a microbiota similar to that of periodontitis-affected teeth. this case report describes the subgingival microbiota of a 45-year-old female with advanced periodontitis before and after complete edentulation and reconstruction with dental implants. a 3-month healing period post extraction passed before implants were placed using a two-stage submerged implant protocol. at 4- to 6-month recall visits after definitive prosthetic reconstruction, some ... | 2009 | 18657153 |
| lemierre's syndrome: methicillin-resistant staphylococcus aureus (mrsa) finds a new home. | lemierre's syndrome is septic thrombophlebitis of the internal jugular vein, arising as a complication of an oropharyngeal infection. this thrombophlebitis frequently results in septic emboli to organs such as the lungs. the causative agent in most previously described cases is fusobacterium necrophorum, an anaerobic gram-negative organism. we present the case of an 8-year-old previously healthy girl who came to the emergency department with a 5-day history of left-sided neck pain and was subseq ... | 2009 | 18280087 |
| clinical and microbiological determinants of ailing dental implants. | the failure of the host tissue to establish or maintain osseointegration around dental implants is due to either occlusal or parafunctional forces, premature loading, ill-directed stress, or microbial infection. the long-term failure rate of dental implants is generally 5-10%. although a variety of etiologies of early peri-implant bone loss (from implant placement to 1-year post-loading) have been proposed, factors associated with late implant failures are less well understood but are probably r ... | 2009 | 18384407 |
| characterization of bacterial flora in persistent apical periodontitis lesions. | microorganisms are able to survive and induce persistent infection in periapical tissues. the aim of this study was to investigate the composition of the microflora of persistent apical periodontitis lesions. | 2009 | 19832803 |
| expand the pharyngitis paradigm for adolescents and young adults. | current guidelines and review articles emphasize that clinicians should consider group a beta-hemolytic streptococcus in the diagnosis and management of patients with acute pharyngitis. recent data suggest that in adolescents and young adults (persons aged 15 to 24 years), fusobacterium necrophorum causes endemic pharyngitis at a rate similar to that of group a beta-hemolytic streptococcus. on the basis of published epidemiologic data, f. necrophorum is estimated to cause the lemierre syndrome-a ... | 2009 | 19949147 |
| lemierre's syndrome and septicaemia caused solely by arcanobacterium haemolyticum in a young immunocompetent patient. | we present a case of septicaemia caused by arcanobacterium haemolyticum in a previously healthy 23-year-old man suffering from acute pharyngotonsillitis, who developed complicated lemierre's syndrome. three blood cultures (both aerobic and anaerobic) revealed the exclusive presence of a. haemolyticum. the presence of fusobacterium necroforum was not essential for the development of this pathology. to our knowledge, this is the first reported case of lemierre's syndrome caused solely by a. haemol ... | 2009 | 19661210 |
| [dyspnea with fever]. | 2009 | 19953051 | |
| kinetic characterization and quaternary structure of glutamate racemase from the periodontal anaerobe fusobacterium nucleatum. | cofactor-independent glutamate racemases (grs) that supply the d-glutamate required for biosynthesis of the peptidoglycan that encapsulates bacterial cells are attractive targets for the development of antibacterial drugs. recombinant gr from fusobacterium nucleatum (fngr), a gram-negative anaerobe involved in periodontal disease, was overproduced, purified, and characterized. unlike most other grs, fngr is a pseudosymmetric enzyme, catalyzing the racemization of glutamate enantiomers with simil ... | 2009 | 19772853 |
| quantitative detection of periodontopathic bacteria in atherosclerotic plaques from coronary arteries. | oral pathogens, including periodontopathic bacteria, are thought to be aetiological factors in the development of cardiovascular disease. in this study, the presence of aggregatibacter actinomycetemcomitans, fusobacterium nucleatum-periodonticum-simiae group, porphyromonas gingivalis, prevotella intermedia, prevotella nigrescens and tannerella forsythia in atheromatous plaques from coronary arteries was determined by real-time pcr. forty-four patients displaying cardiovascular disease were submi ... | 2009 | 19679682 |
| a vanishing disease can still happen. | lemierre's syndrome is a rare disease in the antibiotic era which is characterised by oropharyngitis, thrombophlebitis and septic metastases, but it can still occur and early recognition of its characteristic features and commencement of appropriate treatment can be life saving. the case history is described of a middle-aged man with lemierre's syndrome who made an uneventful recovery following treatment with co-amoxiclav and metronidazole for 6 weeks. | 2009 | 19773506 |
| oral bacteria induce a differential activation of human immunodeficiency virus-1 promoter in t cells, macrophages and dendritic cells. | the human immunodeficiency virus (hiv) can integrate into t cells, macrophages and dendritic cells resulting in a latent infection. reports have also demonstrated that various microbial and host cell factors can trigger hiv reactivation leading to hiv recrudescence, potentially undermining highly active antiretroviral therapies. | 2009 | 19702954 |
| the effect of lactoferrin on oral bacterial attachment. | lactoferrin (lf), an iron-binding salivary glycoprotein, plays an important role in human innate defense against local mucosal infection. we hypothesized that lf interferes with initial oral bacterial attachment to surfaces by iron sequestration, so inhibiting subsequent biofilm formation. the objective was to investigate the effect of lf on the early stages of single-species and multi-species oral biofilm development. | 2009 | 19702956 |
| approaches to the study of the systematics of anaerobic, gram-negative, non-sporeforming rods: current status and perspectives. | the present article gives an overview of recent taxonomic changes among the gram-negative, anaerobic rods, briefly highlighting areas where the biology and ecology have a bearing on recent nomenclatorial changes. the focus is among the genera bacteroides, prevotella, porphyromonas, leptotrichia, dysgonomonas, fusobacterium and the synergistes group and additionally demonstrates the value of conserved indels and group-specific proteins for identifying and circumscribing many of these taxa and the ... | 2009 | 19695337 |
| assessment of intraradicular bacterial composition by terminal restriction fragment length polymorphism analysis. | the aim of the study was to assess the bacterial community structures associated with endodontic infections using terminal restriction fragment length polymorphism (t-rflp), and to investigate the correlation of whole community profiles with the manifestation of particular clinical features. | 2009 | 19702949 |
| purulent pericarditis in patient with esophageal cancer presenting with cardiac tamponade. | 2009 | 19272728 | |
| community-acquired methicillin-resistant staphylococcus aureus and lemierre syndrome. | lemierre syndrome is usually caused by fusobacterium necrophorum, but we recently encountered a case due to methicillin-resistant staphylococcus aureus and complicated by sigmoid sinus thrombosis and cranial neuropathies. therapy may be made more difficult by unusual organisms and also create the need for therapy that penetrates into the central nervous system. | 2009 | 19701080 |
| contemporary root canal irrigants are able to disrupt and eradicate single- and dual-species biofilms. | clinical/microbiological studies have consistently revealed the persistence of some bacteria after conventional root canal debridement. although this was originally attributed to the complexity of the root canal anatomy and the difficulty of delivering antibacterial agents effectively, it has emerged that the biofilm encasement of bacterial cells may confer a further mechanism of resistance. the purpose of this study was to investigate the relative disruption and bactericidal effects of root can ... | 2009 | 19720223 |
| maternal periodontal disease and soluble fms-like tyrosine kinase-1 expression. | this study was conducted to examine the relationship between maternal periodontal disease and plasma angiogenic factor expression of soluble fms-like tyrosine kinase (sflt)-1. | 2009 | 19722802 |
| fusobacterium nucleatum endocarditis mimicking polymyalgia rheumatica. | a case of fusobacterium nucleatum endocarditis in an 80-year-old man is reported. the patient presented with a headache and nonspecific musculoskeletal symptoms and was misdiagnosed as having polymyalgia rheumatica. the diagnosis of bacterial endocarditis was delayed because of an insidious presentation, typical in infections with low virulence micro-organisms. the musculoskeletal symptoms, unresponsive to protracted corticosteroids, completely resolved with intravenous ampicillin treatment. rhe ... | 2009 | 19738533 |
| mutualistic biofilm communities develop with porphyromonas gingivalis and initial, early, and late colonizers of enamel. | porphyromonas gingivalis is present in dental plaque as early as 4 h after tooth cleaning, but it is also associated with periodontal disease, a late-developing event in the microbial successions that characterize daily plaque development. we report here that p. gingivalis atcc 33277 is remarkable in its ability to interact with a variety of initial, early, middle, and late colonizers growing solely on saliva. integration of p. gingivalis into multispecies communities was investigated by using t ... | 2009 | 19749049 |
| [an underestimated throat bacteria]. | 2009 | 19756058 | |
| bone microbial contamination influences autogenous grafting in sinus augmentation. | the oral occurrence of putative microbial pathogens in humans has been documented in health and disease. the presence of periodontopathogens in patients with a history of periodontal disease may have a negative impact on bone regeneration. this investigation was conducted to confirm the presence of periodontal pathogens in bone particles harvested intraorally for maxillary sinus augmentation and to assess the clinical and radiographic outcomes 6 to 12 months after bone augmentation. | 2009 | 19656037 |
| lemierre's syndrome: a serious complication of an odontogenic infection. | necrobacillosis, postanginal septicaemia or lemierre's syndrome is characterised by suppurative thrombophlebitis of the internal jugular vein with embolization to several sites, including the lungs. we report the case of a 38-year-old man who was initially hospitalized because of odontogenic cellulitis. given the deterioration of his clinical state (septic shock and multiple organ failure), neck computed tomography was performed, revealing both cervical and parotid abscesses, and thrombosis of t ... | 2009 | 19300351 |
| does pregnancy have an impact on the subgingival microbiota? | we investigated clinical and subgingival microbiologic changes during pregnancy in 20 consecutive pregnant women > or =18 years not receiving dental care. | 2009 | 19228092 |
| dichelobacter nodosus, fusobacterium necrophorum and the epidemiology of footrot. | footrot is a debilitating disease of sheep resulting in lameness, production losses and suffering. to study the basic bacteriology of the disease, a survey was initiated across commercial farms and non-commercial research flocks to compare the bacteriology of symptomatic footrot infected sheep with healthy asymptomatic sheep. of the 80 farmers initially contacted, 14 collected hoof swabs and returned the swabs by post. following dna extraction, species-specific pcr was used to identify if dichel ... | 2009 | 19239925 |
| lemierre syndrome: a case of a rarely isolated microorganism, staphylococcus auerus. | lemierre syndrome is an extremely rare complication of mild-to-moderate pharyngeal infections that causes septic embolization to the lungs and other distant sites. one-third of cases present a polymicrobial bacteremia, although the most isolated microorganism is fusobacterium necrophorum. a case of postanginal sepsis caused by a rarely isolated microorganism, staphylococcus auerus, in a geriatric patient is reported. | 2009 | 19247251 |
| lysozyme and microbiota in relation to gingivitis and periodontitis. | the aim of this study was to investigate associations between the immune component of the oral cavity, lysozyme, in gingival crevicular fluid and unstimulated saliva, and colonization dynamics of periodontopathogenic bacteria from supra- and subgingival plaque samples in patients with gingivitis or periodontitis. | 2009 | 19179970 |
| lemierre syndrome: must anticoagulation remain controversial? | 2009 | 19265373 | |
| an in vitro evaluation of hydrolytic enzymes as dental plaque control agents. | the plaque-control potential of commercially available amylase, lipase and protease was evaluated by observing their effects on coaggregation and on bacterial viability within various plaque microcosms. a quantitative coaggregation assay indicated that protease significantly inhibited the extent of coaggregation of actinomyces naeslundii and streptococcus oralis (p <0.05) and of porphyromonas gingivalis and s. oralis. amylase significantly (p <0.05) increased the coaggregation of a. naeslundii v ... | 2009 | 19273645 |
| [fusobacterium necrophorum: from tonsillitis to lemierre's syndrome]. | fusobacterium necrophorum plays a role in non-gas-tonsillitis in adolescents and probably also in small children with recurrent otitis media. anaerobic culture on selective anaerobic media is recommended to detect f. necrophorum and treat such patients. denmark sees at least 20 annual cases of lemierre's syndrome with a mortality reaching 9%. early suspicion of lemierre's syndrome in adolescents with non-gas-tonsillitis, who develop septicaemia, pulmonary symptoms and unilateral swelling on the ... | 2009 | 19284917 |
| [can anaerobic culture of throat swabs prevent lemierre's syndrome?]. | six cases of lemierre's syndrome were reported from 2004 to 2007 at viborg hospital, corresponding to 33 cases per year in denmark. all six patients were healthy younger persons presenting with a suspected bacterial tonsillitis which had been found strep a antigen negative. fusobacterium necrophorum was found in throat swabs by anaerobic culture on selective media and/or by real-time pcr. we recommend that all patients 10 to 40 years of age with strep a antigentest negative bacterial tonsillitis ... | 2009 | 19284918 |
| [prior use of anti-inflammatory and antibiotic drugs in patients hospitalized for peritonsillar abscess]. | the purpose of this study was to describe the characteristics of patients hospitalized for a peritonsillar abscess over a 1-year period and to evaluate the proportion of patients exposed to anti-inflammatory and antibiotic drugs before hospitalization. | 2009 | 19345530 |
| characterization of the complex bacterial communities colonizing biliary stents reveals a host-dependent diversity. | this study provides a comprehensive survey of the spatial and temporal bacterial composition of biliary stent biofilms. the bacterial diversity, distribution and dynamics of 59 biliary and 4 pancreatic stent communities from 40 patients being treated at two different hospitals, which implant stents either simultaneously or consecutively, were characterized by single-strand conformation polymorphism (sscp) analysis. fifty-one phylotypes belonging to 5 bacterial phyla and 24 bacterial families wer ... | 2009 | 19360025 |
| [atypical sore throats]. | 2009 | 19167864 | |
| a 40-day siege. | 2009 | 19185090 | |
| orbital abscesses caused by fusobacterium necrophorum. | the authors describe 2 cases of young men who presented with pansinusitis and fusobacterium necrophorum orbital abscesses. the first patient had a complicated clinical course including epidural abscess formation and meningitis. he underwent surgical evacuation of the abscesses and sinus drainage and required long-term broad spectrum antibiotic therapy with full recovery. the second patient had a clinical course complicated by f. necrophorum septicemia. he required surgical evacuation of the absc ... | 2009 | 19273938 |
| [acute mastoiditis in children: can mastoidectomy be avoided?]. | mastoidectomy is the standard management for exteriorized mastoiditis. the objective of this study was to assess the results of conservative management of acute mastoiditis and to study the types of bacteria isolated and their sensitivity to antibiotics. | 2009 | 19524875 |
| bacterial colonization of enamel in situ investigated using fluorescence in situ hybridization. | oral biofilms are one of the greatest challenges in dental research. the present study aimed to investigate initial bacterial colonization of enamel surfaces in situ using fluorescence in situ hybridization (fish) over a 12 h period. for this purpose, bovine enamel slabs were fixed on buccal sites of individual splints worn by six subjects for 2, 6 and 12 h to allow biofilm formation. specimens were processed for fish and evaluated with confocal laser-scanning microscopy, using probes for eubact ... | 2009 | 19528150 |
| epidemiology of pharyngeal carriage of fusobacterium necrophorum. | 2009 | 19528186 | |
| starvation response and growth in serum of fusobacterium nucleatum, peptostreptococcus anaerobius, prevotella intermedia, and pseudoramibacter alactolyticus. | the microbiota inhabiting the untreated root canal differ markedly from those found in post-treatment disease, yet there is limited information on the microbial characteristics distinguishing the different infections. we hypothesized that starvation survival is a key microbial property in species selection. this study analyzed starvation-survival behavior over 60 days of species representative of the untreated root canal infection: fusobacterium nucleatum, peptostreptococcus anaerobius, prevotel ... | 2009 | 19540450 |
| fusobacterium nucleatum enters normal human oral fibroblasts in vitro. | fusobacterium nucleatum, a commensal opportunistic oral bacterium, is capable of invading gingival epithelial cells, but the entrance into human primary oral fibroblast cells has not been documented. this study evaluated the ability of three strains of f. nucleatum (f. nucleatum ssp. nucleatum, f. nucleatum ssp. polymorphum, and f. nucleatum ssp. vincentii) to enter gingival fibroblasts (gfs) and periodontal ligament fibroblasts (plfs). | 2009 | 19563299 |
| comparison between polymerase chain reaction-based and checkerboard dna hybridization techniques for microbial assessment of subgingival plaque samples. | to compare polymerase chain reaction (pcr) with subsequent reverse hybridization (micro-ident test) and checkerboard dna-dna hybridization for the identification of 13 bacterial species in subgingival plaque samples. | 2009 | 19563330 |
| risk assessment of ciprofloxacin, flavomycin, olaquindox and colistin sulfate based on microbiological impact on human gut biota. | trace levels of veterinary antibiotics that reside in livestock products may disturb the balance of human intestinal microbiota and impair the colonized barrier function, which is critical to protect against the invasion or overgrowth of exogenous pathogens. we investigated the colonization barrier disruption effect of ciprofloxacin, flavomycin, olaquindox and colistin sulfate by the minimum inhibitory concentration (mic) assay in pure culture of human gut bacteria and evaluated the no-observed- ... | 2009 | 19545513 |
| aggregatibacter actinomycetemcomitans builds mutualistic biofilm communities with fusobacterium nucleatum and veillonella species in saliva. | human oral bacterial pathogens grow in attached multispecies biofilm communities. unattached cells are quickly removed by swallowing. therefore, surface attachment is essential for growth, and we investigated multispecies community interactions resulting in mutualistic growth on saliva as the sole nutritional source. we used two model systems, saliva-coated transferable solid-phase polystyrene pegs (peg biofilms) and flow cells with saliva-coated glass surfaces. fluorescent antibody staining and ... | 2009 | 19564387 |
| lemierre's syndrome: a systematic review. | lemierre's syndrome is characterized by a history of recent oropharyngeal infection, clinical or radiological evidence of internal jugular vein thrombosis, and isolation of anaerobic pathogens, mainly fusobacterium necrophorum. it was once called the forgotten disease because of its rarity, but it may not be that uncommon after all. this review aims to provide physicians with an update on the etiology, management, and prognosis of lemierre's syndrome. | 2009 | 19554637 |
| a 21-year-old man with fever and abdominal pain after recent peritonsillar abscess drainage. | lemierre syndrome an extremely rare and unreported disease typically results from oropharyngeal infection and/or local tissue trauma with invasion of the parapharyngeal soft tissue with bacteria. once local tissue invasion occurs, internal jugular vein septic thrombophlebitis with or without metastatic complications may occur. the etiology of lemierre syndrome is generally fusobacterium necrophorum, reported to be present in approximately 80% of the cases. the outcome of lemierre syndrome in the ... | 2009 | 19555636 |
| multi-species biofilm formation on dental materials and an adhesive patch. | this laboratory study assessed the influence of surface roughness and contact time on the formation of a multi-species biofilm on dental materials (adhesive patch, composite, amalgam and enamel). | 2009 | 19408815 |
| proteomic analysis of endodontic infections by liquid chromatography-tandem mass spectrometry. | endodontic infections are very prevalent and have a polymicrobial etiology characterized by complex interrelationships between endodontic microorganisms and the host defenses. proteomic analysis of endodontic infections can provide global insights into the invasion, pathogenicity mechanisms, and multifactorial interactions existing between root canal bacteria and the host in the initiation and progression of apical periodontitis. the purpose of this study was to apply proteomic techniques such a ... | 2009 | 19572900 |
| discrimination between bacteroides, dichelobacter, fusobacterium, porphyromonas and prevotella isolated from caprine footrot by pcr-rflp - short communication. | footrot is widely considered the most severe and most common foot pathology in small ruminants. this study tested the ability of a molecular typing system based on polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp) assay of the 16s rrna gene to discriminate between the strict anaerobe genera most commonly isolated from footrot ( bacteroides, dichelobacter, fusobacterium, porphyromonas and prevotella ) in goats in extremadura (spain), with a view to facilitating identif ... | 2009 | 19584033 |
| traditional medicinal plant extracts and natural products with activity against oral bacteria: potential application in the prevention and treatment of oral diseases. | oral diseases are major health problems with dental caries and periodontal diseases among the most important preventable global infectious diseases. oral health influences the general quality of life and poor oral health is linked to chronic conditions and systemic diseases. the association between oral diseases and the oral microbiota is well established. of the more than 750 species of bacteria that inhabit the oral cavity, a number are implicated in oral diseases. the development of dental ca ... | 2009 | 19596745 |
| large scale biomimetic membrane arrays. | to establish planar biomimetic membranes across large scale partition aperture arrays, we created a disposable single-use horizontal chamber design that supports combined optical-electrical measurements. functional lipid bilayers could easily and efficiently be established across co(2) laser micro-structured 8 x 8 aperture partition arrays with average aperture diameters of 301 +/- 5 microm. we addressed the electro-physical properties of the lipid bilayers established across the micro-structure ... | 2009 | 19672582 |
| the detection of dichelobacter nodosus and fusobacterium necrophorum from footrot lesions in new zealand goats. | 2009 | 19248838 | |
| lkta-encoded leukotoxin is not a universal virulence factor in invasive fusobacterium necrophorum infections in animals and man. | 2009 | 19273652 | |
| purification and partial characterization of a bacteriocin produced by an oral fusobacterium nucleatum isolate. | to purify and partially characterize a bacteriocin produced by a fusobacterium nucleatum strain. | 2009 | 19426276 |
| tgf-beta1 inhibits tlr-mediated odontoblast responses to oral bacteria. | tgf-beta1 exerts diverse functions in tooth development and tissue repair, but its role in microbial defenses of the tooth is not well-understood. odontoblasts extending their cellular processes into the dentin are the first cells to recognize signals from tgf-beta1 and bacteria in carious dentin. this study aimed to determine the role of tgf-beta1 in modulating odontoblast responses to oral bacteria. we show that these responses depend upon the expression levels of microbial recognition recepto ... | 2009 | 19407153 |
| bacterial culture and dna checkerboard for the detection of internal contamination in dental implants. | the aim of this in vitro study was to evaluate the bacterial leakage along the implant-abutment interface by the conventional bacterial culture and dna checkerboard hybridization method. | 2009 | 19374709 |
| polymicrobial infections, biofilms, and beyond. | 2009 | 19419439 | |
| the impact of the stone age diet on gingival conditions in the absence of oral hygiene. | the objective of this study was to assess the oral microbiota and clinical data in subjects without access to traditional oral hygiene methods and who ate a diet available in the stone age. | 2009 | 19405829 |
| decreased interleukin-2 responses to fusobacterium nucleatum and porphyromonas gingivalis in generalized aggressive periodontitis. | compromised t-cell responses to periodontal pathogens may contribute to the pathogenesis of generalized aggressive periodontitis (gagp). in this study, we attempted to characterize t-helper cell (th1, th2, and th17) responses in patients with gagp and healthy controls upon stimulation with disease-relevant pathogens. | 2009 | 19405834 |
| mouse model of experimental periodontitis induced by porphyromonas gingivalis/fusobacterium nucleatum infection: bone loss and host response. | to compare the effect of oral infection with porphyromonas gingivalis or fusobacterium nucleatum versus infection with both bacteria on mouse periodontal tissues, and to characterize the inflammatory response. | 2009 | 19419440 |
| a sore throat--potentially life-threatening? | a sore throat, which is most commonly viewed as a minor ailment, can be a manifestation of a life-threatening disorder known as lemierre's syndrome caused by fusobacterium necrophorum. we report a new case of lemierre's syndrome that occurred in an otherwise healthy 18-year-old woman, who initially presented with fever and sore throat. the diagnosis was not made until a week later when blood cultures became available. this syndrome should be suspected until proven otherwise in any patient with s ... | 2009 | 19430936 |
| identification of three immunodominant regions on leukotoxin protein of fusobacterium necrophorum. | to analyze immunodominant regions of leukotoxin protein of fusobacterium necrophorum strain h05, a series of truncated forms of leukotoxin gene were expressed in escherichia coli using the vector pgex-6p-1 or pproex hta. the results of sds-page showed the truncated forms pl1, pl2, pl4, and pl5 were expressed in escherichia coli using the vector pgex-6p-1, and the truncated forms pl3 was expressed in escherichia coli using the vector pproex hta. these recombinant proteins were able to react with ... | 2009 | 19449113 |
| proteomics of porphyromonas gingivalis within a model oral microbial community. | porphyromonas gingivalis is a periodontal pathogen that resides in a complex multispecies microbial biofilm community known as dental plaque. confocal laser scanning microscopy showed that p. gingivalis can assemble into communities in vitro with streptococcus gordonii and fusobacterium nucleatum, common constituents of dental plaque. whole cell quantitative proteomics, along with mutant construction and analysis, were conducted to investigate how p. gingivalis adapts to this three species commu ... | 2009 | 19454014 |
| answer to case of the month #151 lemierre's syndrome. | 2009 | 19591767 | |
| methods for optimizing dna extraction before quantifying oral bacterial numbers by real-time pcr. | methods for the optimal extraction of genomic dna for real-time pcr enumeration of oral bacteria using the muramidase, mutanolysin, were developed using a simple in vitro oral flora model comprised of the facultative anaerobic gram-positive bacteria, lactobacillus acidophilus and streptococcus mutans, the gram-positive anaerobe, parvimonas micra, and the gram-negative anaerobes, porphyromonas gingivalis, prevotella melaninogenica and fusobacterium nucleatum. traditional, as well as more elaborat ... | 2009 | 19459962 |
| the bacteriology of salivary gland infections. | the parotid gland is the salivary gland most commonly affected by inflammation. however, infection of the salivary glands can occur in any of the glands. the most common pathogens associated with acute bacterial infection are staphylococcus aureus and anaerobic bacteria. the predominant anaerobes include: anaerobic gram negative bacilli (eg, pigmented prevotella and porphyromonas); fusobacterium spp; and peptostreptococcus spp. in addition, streptococcus spp (including streptococcus pneumoniae) ... | 2009 | 19608044 |
| "a forgotten disease": a case of lemierre syndrome. | lemierre's syndrome is a rare but a life threatening condition which affects young healthy individuals, was first described by dr.andre lemierre in 1936. incidence rates are between 0.6 and 2.3 per million population. it is found more commonly in males, with a male to female ratio of approximately 2:1. its pathogenesis consists of the development of infectious thrombophlebitis in the internal jugular vein or one of its branches caused by a focal sepsis, mostly localized in the oropharynx, leadin ... | 2009 | 19468652 |
| fusobacterium nucleatum, the first gram-negative bacterium demonstrated to produce polyglutamate. | poly-gamma-glutamate has been described in many gram-positive organisms. when anchored to the surface, it is a capsule and as such a virulence factor. based on sequence similarities, few gram-negative organisms have been suggested to synthesize poly-gamma-glutamate. for the first time, a gram-negative bacterium, fusobacterium nucleatum, is shown to produce and secrete poly-gamma-glutamate. putative poly-gamma-glutamate-synthesizing genes from gram-negative organisms have been compared with their ... | 2009 | 19483793 |
| [preliminary study on the discrimination of putative periodontal pathogens with a metabonomics method]. | to evaluate the feasibility of identifying oral pathogenic bacteria by comparing the metabolic profiling of putative periodontal pathogens and try to find a convenient and rapid way to discriminate oral microorganisms. | 2009 | 19637485 |
| seroprevalence of fusobacterium varium in ulcerative colitis patients in japan. | the etiology of ulcerative colitis (uc) is unknown, while an exacerbating factor of this disease is associated with infectious agents. recently, fusobacterium varium has been found in the mucosa of a significant number of patients with uc. the aim of this study was to estimate the prevalence of f. varium infection based on serology, evaluate the relationship between f. varium seropositivity and uc, and determine the clinical characteristics of infected uc individuals. seropositive patients were ... | 2009 | 19484811 |
| fetal exposure to oral pathogens and subsequent risk for neonatal intensive care admission. | maternal periodontal infection has been associated with adverse maternal and neonatal outcomes. in utero fetal exposure to oral pathogens was also recognized as deleterious to the fetus. the objective of this study was to determine the relationship between fetal exposure to oral pathogens and neonatal intensive care unit (nicu) admission. | 2009 | 19485816 |
| bite-related and septic syndromes caused by cats and dogs. | bite infections can contain a mix of anaerobes and aerobes from the patient's skin and the animal's oral cavity, including species of pasteurella, streptococcus, fusobacterium, and capnocytophaga. domestic cat and dog bite wounds can produce substantial morbidity and often require specialised care techniques and specific antibiotic therapy. bite wounds can be complicated by sepsis. disseminated infections, particularly those caused by capnocytophaga canimorsus and pasteurella multocida, can lead ... | 2009 | 19555903 |
| systemic immune responses in pregnancy and periodontitis: relationship to pregnancy outcomes in the obstetrics and periodontal therapy (opt) study. | our previous studies reported on the obstetric, periodontal, and microbiologic outcomes of women participating in the obstetrics and periodontal therapy (opt) study. this article describes the systemic antibody responses to selected periodontal bacteria in the same patients. | 2009 | 19485826 |
| [detection of luxs gene of quorum sensing signal system in periodontal pathogen]. | to detect the presence and distribution of luxs gene in quorum sensing signal system in the periodontal pathogens. | 2009 | 19489256 |
| unusual presentation of lemierre's syndrome: two cases and a review. | lemierre's syndrome is a potentially fatal disease that usually presents with oropharyngeal infection, followed by sepsis, thrombosis of the internal jugular vein and septic emboli. most cases are caused by the gram-negative, anaerobic fusobacterium necrophorum. we present two patients with an atypical presentation of lemierre's syndrome and a review. these cases illustrate that a positive blood culture for f. necrophorum, even without the presence of clinical symptoms pointing towards thrombosi ... | 2009 | 19491663 |
| real-time polymerase chain reaction to determine the prevalence and copy number of epstein-barr virus and cytomegalovirus dna in subgingival plaque at individual healthy and periodontal disease sites. | detection of cytomegalovirus (cmv) and epstein-barr virus (ebv) in plaque from patients with periodontal disease provides support for the theory that these viruses play a role in the pathogenesis of periodontitis. this study sought to further define this relationship by determining the prevalence of these viruses at individual disease and healthy sites of patients with periodontal disease and to determine whether the presence and amount of viral dna correlate with disease severity. | 2009 | 19563294 |
| variation in fusobacterium necrophorum strains present on the hooves of footrot infected sheep, goats and cattle. | footrot is a disease of sheep, goats and cattle that causes losses in production and raises welfare issues world-wide. the disease is characterised by destruction of the hard keratin of the hoof leading to lameness, and both dichelobacter nodosus (d. nodosus) and fusobacterium necrophorum (f. necrophorum) are thought to be involved in the etiology of this disease. while a lot is known about the genetic diversity of d. nodosus, very little is known about variation in f. necrophorum, especially as ... | 2009 | 19019570 |
| oral bacteria modulate invasion and induction of apoptosis in hep-2 cells by pseudomonas aeruginosa. | pseudomonas aeruginosa is an important opportunistic bacterial pathogen, causing infections of the respiratory and other organ systems in susceptible hosts. p. aeruginosa infection is initiated by adhesion to and invasion of mucosal epithelial cells. the failure of host defenses to eliminate p. aeruginosa from mucosal surfaces results in p. aeruginosa proliferation, sometimes followed by overt infection and tissue destruction. there is growing evidence that associates poor oral health and respir ... | 2009 | 19041936 |
| characterization of coaggregation of fusobacterium nucleatum pk1594 with six porphyromonas gingivalis strains. | coaggregation is a key mechanism in biofilm formation. the aim of this study was to characterize the coaggregation of fusobacterium nucleatum pk1594 with six porphyromonas gingivalis strains in terms of kinetics and sugars inhibition. this coaggregation was quantitatively characterized by using a kinetic coaggregation assay. sugar inhibition profiles were also quantitatively defined. four types of interactions among these coaggregation partners were found: (1) fast coaggregation that was substan ... | 2009 | 19084124 |
| tinidazole inhibitory and cidal activity against anaerobic periodontal pathogens. | the in vitro activity of tinidazole against anaerobic periodontal pathogens (25 prevotella buccae, 18 prevotella denticola, 10 prevotella intermedia, 6 prevotella melaninogenica, 5 prevotella oralis, 10 fusobacterium nucleatum and 8 veillonella spp.) was determined by agar dilution. mic(90) values (minimum inhibitory concentration for 90% of the organisms) were 8 microg/ml for veillonella spp., 4 microg/ml for p. intermedia, 2 microg/ml for p. buccae, 1 microg/ml for fusobacterium spp. and 0.5 m ... | 2009 | 19097755 |
| toll-like receptor 2 and nalp2 mediate induction of human beta-defensins by fusobacterium nucleatum in gingival epithelial cells. | we previously reported that infection by fusobacterium nucleatum strongly induced the expression of both human beta-defensin 2 (hbd-2) and hbd-3 by gingival epithelial cells. the aim of this study was to characterize the pattern recognition receptors (prrs) and regulatory mechanisms involved in the induction of hbd-2 and -3 expression by f. nucleatum in gingival epithelial cells. immortalized human gingival epithelial hok-16b cells were infected with live or heat-killed f. nucleatum, and the exp ... | 2009 | 19103770 |
| two teenagers with sepsis and pneumonia. | 2009 | 19114923 |