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molecular characterization of avian strains of pasteurella multocida serogroup-a:1 based on amplification of repetitive regions by pcr.repetitive extragenic palindromic (rep)-pcr (polymerase chain reaction), enterobacterial repetitive intergenic consensus (eric)-pcr, and single primer pcr assays were employed to characterize 66 strains of pasteurella multocida serogroup a:1 isolated from avian species belonging to different regions of india. rep-pcr resulted in amplification of rep sequences from the genome which were in the range of approximately 200 to approximately 3000 bp and accounted for a total of 54 distinguishing profi ...200817537513
an investigation of the aetiological role of bovine herpesvirus 4 in bovine endometritis.uteri from 31 infertile cattle were examined for the presence of bovine herpesvirus 4 (bohv-4) by nested polymerase chain reaction (pcr). samples were also tested for bacteria, including chlamydiae and mycoplasma bovis. bohv-4 was detected by pcr in 27/31 (87.1%) samples, but the presence and amount of viral dna was not correlated with histological and bacteriological findings. arcanobacterium pyogenes, histophilus somni and pasteurella multocida were isolated from five cows with endometritis. c ...200817572123
ompa: gating and dynamics via molecular dynamics simulations.outer membrane proteins (omps) of gram-negative bacteria have a variety of functions including passive transport, active transport, catalysis, pathogenesis and signal transduction. whilst the structures of approximately 25 omps are currently known, there is relatively little known about their dynamics in different environments. the outer membrane protein, ompa from escherichia coli has been studied extensively in different environments both experimentally and computationally, and thus provides a ...200817601489
experimental study of pathogenicity of pasteurella multocida serogroup f in rabbits.the role of pasteurella multocida serogroup f in inducing disease in rabbits was investigated in this study. three groups of 12 pasteurella-free rabbits each were intranasally (i.n.), subcutaneously (s.c.), and perorally (p.o.) challenged, respectively. six rabbits of each group were immunosuppressed using dexamethasone. eight rabbits (four of them immunosuppressed) inoculated i.n. showed symptoms of respiratory distress resulting in respiratory failure and died or were euthanized in the termina ...200817629636
invasive pasteurella multocida sinusitis in a renal transplant patient.pasteurella are commensal gram-negative bacteria isolated from the oral cavity of many domesticated animals. most human infections occur post animal bite or scratch injury resulting in local cutaneous infection; however, case reports suggest that transmission may occur via animal secretions. pasteurella species can be associated with serious systemic infections particularly in those with underlying disease and in the immunocompromised. we present a case of invasive pasteurella multocida sinusiti ...200817655653
pcr analysis of pasteurella multocida isolates from an outbreak of pasteurellosis in indian pigs.an outbreak of pasteurellosis with high mortality was recorded in indigenous pigs in india. the presence of pasteurella multocida in samples collected from dead pigs was detected by smear examination and isolation, and later by p. multocida specific polymerase chain reaction (pm-pcr). p. multocida was detected in all the samples collected from dead pigs, with nine strains ultimately isolated. all the isolates were positive by pm-pcr. six isolates showed capa and three were of capd capsular types ...200817669491
breast prosthesis infected by pasteurella multocida. 200819186571
pasteurella abscess in tibial regenerate bone: a case report.although superficial pin tract infections during limb lengthening are extremely common, most settle spontaneously or with antibiotics. in contrast, deep infection around pin tracts leading to osteomyelitis and abscess formation is much less frequently observed and may need washout and curettage. we report a case of a 10-year-old boy who presented with latent abscess formation at a tibial lengthening site because of pasteurella, a rare bacterial cause of infection usually associated with animals. ...200819471175
pattern classification of phylogeny signals.in this paper we propose the minimum entropy clustering (mec) method for clustering genes based on their phylogenetic signals. this entropy based method will cluster two genes together when their concatenation can decrease the entropy. an integral feature of mec is that it chooses the number of clusters automatically, which is a major advantage over the other methods. our simulation results show that this method is quite successful in clustering genes with a common phylogeny.200818976226
routine phenotypic identification of bacterial species of the family pasteurellaceae isolated from animals.pasteurellaceae are bacteria with an important role as primary or opportunistic, mainly respiratory, pathogens in domestic and wild animals. some species of pasteurellaceae cause severe diseases with high economic losses in commercial animal husbandry and are of great diagnostic concern. because of new data on the phylogeny of pasteurellaceae, their taxonomy has recently been revised profoundly, thus requiring an improved phenotypic differentiation procedure to identify the individual species of ...200818987220
[expression and purification of an adhesive protein of rabbit pasteurella multocida c51-3 and detection of its antigenicity].the cp36 gene encoding an adhesive protein was amplified by pcr from genomic dna of rabbit p. multocida c51-3 strain, and cloned into the pmd18-t vector and then sequenced. the mature adhesive protein without a signal peptide of cpm36 gene was amplified by pcr from the recombinant plasmid pmd18-cp36, then cloned into the prokaryotic expression vector pqe30 to provide a recombinant plasmid pqe30-cpm36. the recombinant protein of cpm36 was produced in escherichia coli m15 harboring the recombinant ...200818998549
efficacy of metaphylactic florfenicol therapy during natural outbreaks of bovine respiratory disease.the efficacy of an injectable formulation of florfenicol (300 mg/ml) as metaphylactic control of naturally occurring bovine respiratory disease (brd) was evaluated in two double-blind randomly controlled field studies on two dutch veal calf herds (a and b). cattle aged not older than 3 months and in the direct presence of calves with clinical respiratory disease were randomly allocated to treatment with 40 mg/kg florfenicol subcutaneously (s.c.) a positive control treatment (12.5 mg/kg tilmicosi ...200819000268
in vitro activities of tulathromycin and ceftiofur combined with other antimicrobial agents using bovine pasteurella multocida and mannheimia haemolytica isolates.the purpose of this study was to determine the activities of two antibacterial agents used in the treatment of bovine respiratory infections-tulathromycin, a macrolide, and ceftiofur, a third-generation cephalosporin-alone, in combination with each other, and in combination with each of seven additional antibiotics (tilmicosin, florfenicol, enrofloxacin, danofloxacin, ampicillin, tetracycline, and penicillin g) against bovine pasteurella multocida (n = 60) and mannheimia haemolytica (n = 10) iso ...200819003782
[animal bites and infection].animal bites, mostly dog bites, often require an emergency department visit, but are often mistakenly considered innocuous. indeed, bite wounds can lead to an handicap as well as become infected, mostly with the oral flora of the biting animal (pasteurella, capnocytophaga, eikennella, streptococci, staphylococci and anaerobic bacteria). bites can also cause other infections like tetanus, rabies, cat scratch disease, tularemia, leptospirosis, rat-bite fever and plague. the management of bite woun ...200819009844
pasteurella multocida sepsis--should immunocompromised patients give up their pets? 200818847171
recurrent septicemia and osteomyelitis caused by pasteurella multocida in a patient with chronic lymphatic leukemia. 200818847173
[zoonoses transmitted by mouse and rat maintained as laboratory or pet animals].large numbers of mice (mus spp.) and rats (rattus spp.) are maintained for scientific reasons and as pet animals in germany. while laboratory animals are monitored for pathogenic agents, the hygienic status of pet animals is usually completely unknown. despite great efforts, zoonotic infections were reported even in laboratory settings, e.g. with hantavirus (seoul virus), streptobacillus moniliformis, and trichphyton mentagrophytes. however, in current reports, zoonotic infections were transmitt ...200818712260
bacterial cmp-sialic acid synthetases: production, properties, and applications.sialic acids are abundant nine-carbon sugars expressed terminally on glycoconjugates of eukaryotic cells and are crucial for a variety of cell biological functions such as cell-cell adhesion, intracellular signaling, and in regulation of glycoproteins stability. in bacteria, n-acetylneuraminic acid (neu5ac) polymers are important virulence factors. cytidine 5'-monophosphate (cmp)-n-acetylneuraminic acid synthetase (css; ec 2.7.7.43), the key enzyme that synthesizes cmp-n-acetylneuraminic acid, t ...200818716769
characterization of tolc efflux pump proteins from pasteurella multocida.two tolc homologs, pm0527 and pm1980, were identified for pasteurella multocida. a pm0527 mutant displayed increased susceptibility to a range of chemicals, including rifampin (512-fold) and acridine orange (128-fold). a pm1980 mutant showed increased susceptibility to rifampin, ceftazidime, and vancomycin.200818725450
molecular analysis of florfenicol-resistant pasteurella multocida isolates in germany.three florfenicol-resistant pasteurella multocida isolates from germany, two from swine and one from a calf, were investigated for the genetics and transferability of florfenicol resistance.200818786941
fatal pneumonia epizootic in musk ox (ovibos moschatus) in a period of extraordinary weather conditions.the musk ox is adapted to extreme cold and regarded as vulnerable to the impacts of climate change. population decline is proposed to occur due to changes in forage availability, insect harassment, parasite load, and habitat availability, while the possible role of infectious diseases has not been emphasized. the goal of the present article is to describe an outbreak of fatal pasteurellosis that occurred in the introduced musk ox population of dovrefjell, norway in 2006, causing the death of a l ...200818787923
sepsis of total knee arthroplasty after domestic cat bite: should we warn patients? 200818795185
influence of systemic fluoroquinolone administration on the presence of pasteurella multocida in the upper respiratory tract of clinically healthy calves.the influence of enrofloxacin administration (5 mg/kg) for five consecutive days on the occurrence of pasteurella multocida in the upper respiratory tract of two healthy calves was monitored over a 10-day period. from nasal swabs of two additional healthy control calves, which received a placebo saline administration, p. multocida was isolated throughout the study period. in the enrofloxacin treated calves, p. multocida was not demonstrated in the nasopharynx from 48 h after the first injection ...200818808692
why your housecat's trite little bite could cause you quite a fright: a study of domestic felines on the occurrence and antibiotic susceptibility of pasteurella multocida.approximately four to five million animal bite wounds are reported in the usa each year. domestic companion animals inflict the majority of these wounds. although canine bites far outnumber feline bites, unlike the dog, the cat's bite is worse than its bark; 20-80% of all cat bites will become infected, compared with only 3-18% of dog bite wounds. pasteurella multocida is the most commonly cultured bacterium from infected cat bite wounds. anyone seeking medical attention for a cat-inflicted bite ...200818811910
molecular identification of streptococcus equi subsp. equi and streptococcus equi subsp. zooepidemicus in nasal swabs samples from horses suffering respiratory infections in iran.the objective of this study was to evaluate the existence of streptococcus equi subsp. equi and streptococcus equi subsp. zooepidemicus as probable agents associated with naturally occurring infection of the equine upper respiratory disease in mashhad area. nasal swabs samples from thirty horses with upper respiratory tract infections were collected. the bacteria isolated and identified were streptococcus equi subsp. equi (1 isolate), streptococcus equi subsp. zooepidemicus (25 isolates), pasteu ...200818817175
fatal fulminant sepsis due to a cat bite in an immunocompromised patient.pasteurella multocida infections in humans can present as localized infections of soft tissues surrounding the lesions, as respiratory tract infections or as systemic infections with slow or fulminant development. over 90% of human infections are cases of wound infections or abscesses related to a bite, scratch, or licking of skin lesions by a cat or dog. severe systemic diseases such as pneumonia, lung abscess, peritonitis, endocarditis, meningitis and sepsis are also well known, especially in ...200818820856
[pyothorax in 26 cats: clinical signs, laboratory results and therapy (2000-2007)].common historical findings in 26 cats with pyothorax were dyspnea (85%), lethargy (65%), and anorexia (62%), common clinical findings were dyspnea (89%), tachypnea (73%), fever (39%), and hypothermia (27%). frequent laboratory abnormalities were leukocytosis (68%) with a left shift (100%), anemia (65%), hypoalbuminemia (91%), hyperglobulinemia (86%), hyperbilirubinemia (60%) as well as azotemia (52%). the thoracic effusion was purulent in all cats, the thoracic fluid evaluation (n = 19) met the ...200818822608
[fatty acid composition of cells and lipopolysaccliarides of mannheimia haemolytica, mannheimia glucosida and bibersteinia trehalosi strains].the mannheimia haemolytica, mannheimia glucosida and bibersteinia trehalosi strains and the similar fatty acid composition of cells with domination of c(16:1) and c(16:0), which were in almost equal quantities, c(14:0 and c(18:1) + c(18:2). the fatty acid composition of lipopolysaccharides (lps) of the studied bacteria had no essential differences too. it was mainly represented by c(14:0) and 3-oh-c(14:0) which consisted of more than 80% of all lps fatty acids. c(12:0), c(16:1) and c(16:0) were ...200819351048
[primary bacteremias caused by pasteurella multocida]. 200819296005
[pasteurella multocida bacteremic meningitis].human infections by pasteurella multocida are usually associated with bites or scratches from dogs and cats. many of them are accompanied by other oropharyngeal microorganisms of these animals. we herein present a case of bacteremic meningitis by p. multocida in an 86-year-old woman who was living with seven cats. even though no skin or soft tissue infection was recorded, it is possible that a mild infection had gone undetected and a subsequent bacteremia had impacted on the meninges, or that me ...200819213242
cervical spine injury: tiger attack.reports of tiger attacks in the united states are rare. this article presents a case of a young woman who was violently attacked by a siberian tiger and sustained penetrating trauma to the neck, cervical spine, and bilateral lower extremities. this article presents both diagnostic and therapeutic management of patients who may present with similar injuries. animal bites from large animals are prone to infection in 10% to 20% of cases. most infections are polymicrobial, with pasteurella multicida ...200819226051
[occupational exposure to mesophilic microorganisms associated with commercial processing of compost for mushroom production].the aim of the study was to assess the occupational exposure to mesophilic microorganisms associated with commercial processing of compost for mushroom production.200819227882
pasteurellaceae come1 proteins combine the properties of fibronectin adhesins and dna binding competence proteins.a novel fibronectin-binding protein from pasteurella multocida (pm1665) that binds to the fibronectin type iii(9-10) modules via two helix-hairpin-helix motifs has recently been described [1]. this protein shares homology with competence-related dna-binding and uptake proteins (comea and come) from gram-positive and gram-negative bacteria. here, we show that recombinant pm1665 (now designated come1) also binds to dna through the same helix-hairpin-helix motifs required for fibronectin-binding. t ...200819098981
[84-year old woman with necrotic ulcer on the left lower limb]. 200819100137
antibiograms of pathogenic bacteria isolated from laboratory rabbits in ibadan, nigeria.antibiotic sensitivity patterns of 21 bacterial isolates from some clinically ill new zealand rabbits were evaluated against 12 commonly used antibiotics by the kirby-bauer disk-diffusion sensitivity testing method. the 21 isolates consisted of six bordetella bronchiseptica, eight pasteurella multocida, four staphylococcus aureus and three pseudomonas alcaligenes that were associated with snuffles, pneumonia, otitis media, genital infections and conjunctivitis in these groups of caged rabbits. t ...200818840619
safety and efficacy testing of a novel multivalent bovine bacterial respiratory vaccine composed of five bacterins and two immunogens.bovine bacterial respiratory diseases have been one of the most serious problems due to their high mortality and economic loss in calves. the vaccinations of bovine bacterial respiratory vaccines have been complex because of no multivalent vaccine. in this study, novel multivalent bovine bacterial respiratory vaccine (brv) was developed and tested for its safety and efficacy. brv was composed of two immunogens and five bacterins. these were leukotoxoid and bacterin of mannheimia haemolytica type ...200818840971
characterisation and comparison of avian pasteurella multocida strains by conventional and eric-pcr assays.sixty-one avian strains of pasteurella multocida were characterised and compared by biochemical tests, capsular pcr typing and eric-pcr. the strains were recovered from various avian species (goose, duck, muscovy duck, turkey, chicken and pheasant) and represented different geographic locations in hungary. forty-two strains (69%) were identified as p. multocida subsp. multocida and 19 strains (31%) as p. multocida subsp. septica. the strains were grouped into 7 different biovars (1, 2, 3, 4, 5, ...200819149098
recurrent infective endocarditis with uncommon gram-negative pasteurella multocida and pseudomonas aeruginosa: a case report.infective endocarditis (ie) due to gram-negative bacteria is a rare occurrence, with a relative frequency of less than 10% compared to that caused by gram-positive bacteria. herein is presented the fatal case of a 66-year-old man who had undergone mechanical aortic valve replacement 10 years previously, and developed aortic valve ie after sepsis with pasteurella multocida caused by a cat bite at the left medial ankle. in addition, the patient suffered five months later from mitral and aortic val ...200819137805
review article: animal bites: an update for management with a focus on infections.animal bites are a significant public health problem, with an estimated 2% of the population bitten each year. the majority of bites are from dogs and risk factors include young children, men, certain dog breeds and unrestrained dogs. the risk of infection following bites differs among animal species and is dependent on animal dentition and oral flora. recent studies have demonstrated a broad range of pathogens isolated from infected bite wounds, with pasteurella species being the predominant is ...200819125823
action of pasteurella multocida toxin on galpha(q) is persistent and independent of interaction with g-protein-coupled receptors.pasteurella multocida toxin (pmt) activates galpha(q) and facilitates stimulation of inositol phosphate accumulation induced by agonists via g(q)-coupled membrane receptors. here, we studied the effects of pmt on agonist-induced gtpgammas binding to g(q) in cell membranes and a role of g-protein-coupled receptors in the action of pmt. pre-treatment of swiss 3t3 cells with pmt increased bombesin or vasopressin-induced gtpgammas-binding in cell membranes by about 50 to 150%. increase in agonist-st ...200717669624
purification, crystallization and preliminary crystallographic characterization of the alpha 2,6-sialyltransferase from photobacterium sp. jt-ish-224.sialyltransferases transfer sialic acid from cytidine-5-monophospho-n-acetylneuraminic acid (cmp-neuac) to the nonreducing termini of the oligosaccharyl structures of various glycoproteins and glycolipids. the newly cloned alpha2,6-sialyltransferase from photobacterium sp. jt-ish-224 (from the vibrionaceae family) is composed of two domains: an unknown n-terminal domain and a catalytic c-terminal domain which shares significant homology with the pasteurella multocida multifunctional sialyltransf ...200717671362
variation in the molecular weight of photobacterium damselae subsp. piscicida antigens when cultured under different conditions in vitro.the antigenicity of photobacterium damselae (ph. d.) subsp. piscicida, cultured in four different growth media [tryptone soya broth (tsb), glucose-rich medium (grm), iron-depleted tsb (tsb + ir(-)), and iron-depleted grm (grm + ir(-))] was compared by enzyme-linked immunosorbent assay (elisa) and western blot analysis using sera obtained from sea bass (dicentrarchus labrax) raised against live or heat-killed ph. d. subsp. piscicida. the antigenic expression of ph. d. subsp. piscicida was found t ...200717679772
pasteurella multocida septic shock following liver transplantation treated with drotrecogin alpha (activated).severe sepsis and progression to septic shock in solid organ transplant recipients is associated with a high mortality. we describe a fulminant case of septic shock in a liver transplant recipient caused by pasteurella multocida, a gram-negative coccobacillus most commonly associated with domestic cats and dogs. p. multocida is a rare cause of bacteremia and has not been reported as a cause of septic shock following liver transplantation. in addition to standard therapy, the patient was managed ...200717692071
[knee prosthesis infection due to pasteurella multocida]. 200717692220
peritonitis associated with pasteurella multocida in peritoneal dialysis patients--case report and review of the literature.pasteurella multocida is a zoonotic pathogen found in the oral cavities of domestic dogs and cats and other wild and domestic animals. 14 cases ofperitoneal dialysis-associated pasteurella multocida peritonitis linked to animal contact have been reported in the literature to date. in each case, the source of infection was believed to be a domestic cat or cats. one case of spontaneous bacterial peritonitis has been reported in a cirrhotic patient and was not linked to animal contact. poor hygiene ...200717703837
nucleotide sequence and transfer properties of two novel types of actinobacillus pleuropneumoniae plasmids carrying the tetracycline resistance gene tet(h).to analyse the sequence and transfer properties of two tetracycline resistance plasmids found in clinical isolates of actinobacillus pleuropneumoniae in order to assess their role in the spread of tetracycline resistance.200717704116
decoration of pasteurella multocida lipopolysaccharide with phosphocholine is important for virulence.phosphocholine (pcho) is an important substituent of surface structures expressed by a number of bacterial pathogens. its role in virulence has been investigated in several species, in which it has been shown to play a role in bacterial adhesion to mucosal surfaces, in resistance to antimicrobial peptides, or in sensitivity to complement-mediated killing. the lipopolysaccharide (lps) structure of pasteurella multocida strain pm70, whose genome sequence is known, has recently been determined and ...200717704225
recombinant synthesis of hyaluronan by agrobacterium sp.hyaluronan (ha) is a sugar polymer of a repeating disaccharide, beta1-3 d-n-acetylglucosamine (glcnac) beta1-4 d-glucuronic acid (glca). it finds applications in numerous biomedical procedures such as ophthalmic surgery and osteoarthritis treatment. until recently, the only commercial sources were extraction of rooster combs and from fermentation of pathogenic streptococcus. in this work, we demonstrate that metabolic engineering strategies enable the recombinant synthesis of hyaluronan in a saf ...200717705506
contamination of post-endoscopic sinus surgery sinus cavities with pasteurella multocida. 200717711761
pasteurella multocida sepsis and meningitis in 2-month-old twin infants after household exposure to a slaughtered sheep.this article outlines 2 cases of pasteurella multocida sepsis and meningitis in 2-month-old twins after their father was exposed to a sheep during a sacrifice celebration at home. these cases emphasize the necessity of respecting basic hygiene rules and the danger involved in animal sacrifice without suitable professional structure.200717712748
biochemical characterization of a neisseria meningitidis polysialyltransferase reveals novel functional motifs in bacterial sialyltransferases.the extracellular polysaccharide capsule is an essential virulence factor of neisseria meningitidis, a leading cause of severe bacterial meningitis and sepsis. serogroup b strains, the primary disease causing isolates in europe and america, are encapsulated in alpha-2,8 polysialic acid (polysia). the capsular polymer is synthesized from activated sialic acid by action of a membrane-associated polysialyltransferase (nmb-polyst). here we present a comprehensive characterization of nmb-polyst. diff ...200717662040
occurrence of antimicrobial resistance in bacteria from diagnostic samples from dogs.to study the occurrence of antimicrobial resistance among common bacterial pathogens from dogs and relate resistance patterns to data on consumption of antimicrobials.200717644533
evaluation of an indirect enzyme-linked immunosorbent assay (elisa) using recombinant toxin for detection of antibodies against pasteurella multocida toxin.to facilitate the control of progressive atrophic rhinitis (par) of swine caused by toxigenic pasteurella multocida, an enzyme-linked immunosorbent assay (elisa) and a serum neutralization test (nt) have recently been developed to detect antibodies against the p. multocida dermonecrotic toxin (pmdnt). however, the nt is a cumbersome and time-consuming technique. to overcome these drawbacks, we developed an indirect elisa, using recombinant pmdnt expressed in escherichia coli, for the detection o ...200717611352
nucleoside analogues are activated by bacterial deoxyribonucleoside kinases in a species-specific manner.to investigate the bactericidal activity of antiviral and anticancer nucleoside analogues against a variety of pathogenic bacteria and characterize the activating enzymes, deoxyribonucleoside kinases (dnks).200717615154
clinical and in vitro efficacy of amoxicillin against bacteria associated with feline skin wounds and abscesses.a clinical trial involving 122 cats with infected skin wounds or abscesses presented to 10 veterinary clinics was conducted to evaluate the efficacy of 2 oral amoxicillin drug products (a paste and a suspension). a 2nd objective of the study was to identify bacteria involved in such infections and verify their in vitro sensitivity to amoxicillin. samples of wound exudate were harvested at the time of presentation and submitted for aerobic and anaerobic culture. the sensitivity to amoxicillin of ...200717616057
chemoenzymatic synthesis with distinct pasteurella heparosan synthases: monodisperse polymers and unnatural structures.heparosan (-glcua-beta1,4-glcnac-alpha1,4-)(n) is a member of the glycosaminoglycan polysaccharide family found in the capsule of certain pathogenic bacteria as well as the precursor for the vertebrate polymers, heparin and heparan sulfate. the two heparosan synthases from the gram-negative bacteria pasteurella multocida, pmhs1 and pmhs2, were efficiently expressed and purified using maltose-binding protein fusion constructs. these relatively homologous synthases displayed distinct catalytic cha ...200717627940
calcineurin-independent inhibition of 3t3-l1 adipogenesis by pasteurella multocida toxin: suppression of notch1, stabilization of beta-catenin and pre-adipocyte factor 1.pasteurella multocida toxin (pmt) is a potent mitogen and a specific activator of gq-dependent signalling pathways. pmt impairs osteoblast differentiation and causes bone loss and fat reduction in vivo. we examined the effect of pmt on cell signalling pathways involved in 3t3-l1 adipocyte differentiation. we demonstrate that pmt treatment before or together with differentiation induction factors inhibits adipogenesis and prevents upregulation of important adipocyte markers - peroxisome-prolifera ...200717581254
detection of pathogenic leptospires in animals by pcr based on lipl21 and lipl32 genes.efficacy of primers capable of amplifying conserved outer membrane protein (omp) genes i.e., lipl21 and lipl32 of leptospira strains was tested for rapid and early diagnosis of the leptospirosis using a polymerase chain reaction (pcr). these omp genes were found to be conserved in various leptospiral serovars viz., canicola, pomona, icterohaemorrhagiae, pyrogenes, sejroe, grippotyphosa, ballum and tarassovi as pcr products of 561 bp and 756 bp were obtained by pcr employing lipl21 and lipl32 bas ...200717585694
immune response in mice and swine to dna vaccines derived from the pasteurella multocida toxin gene.dna vaccines were constructed with either a 5'-truncated or full-length, genetically detoxified toxin gene from pasteurella multocida and two different dna vaccine vectors, distinguished by the presence or absence of a secretion signal sequence. optimal pmt-specific antibody responses and spleen cell secretion of interferon-gamma following immunization of mice were achieved with pmm4, the construct containing a signal sequence and encoding the entire toxin. antibody responses were also induced i ...200717590484
[monitoring of infectious diseases among wild boars].sixty-seven serum samples and 43 pathological material samples from wild boars, taken in 5 regions of russia and in the kharkov region of the ukraine in 2002 to 2005 were studied. wild boars in some regions of russia were shown to be carriers of aujeszky's disease virus, porcine parvovirus, porcine circovirus type 2, lymphotropic herpesvirus-1, porcine cytomegalovirus, mycoplasma hyopneumoniae and pasteurella multocida. the classical swine fever (csf) virus genome was detected by polymerase chai ...200717601049
the cloning and sequencing of the udp-galactose 4-epimerase gene (gale) from avibacterium paragallinarum.the putative uridine diphosphate (udp)-galactose 4-epimerase encoding gene, gale, was isolated from avibacterium paragallinarum with the use of degenerate primers, colony hybridization and inverse pcr. the data revealed an open reading frame of 1017 bp encoding a protein of 338 amino acids with a molecular weight of 37 kda and an isoelectric point of 5.5. high sequence homology was obtained with an 87, 91 and 89% sequence identity on protein level towards the gale genes from actinobacillus pleur ...200717541831
efficacy of danofloxacin in the treatment of pneumonic pasteurellosis in specific pathogen-free lambs. 200717545648
outer membrane permeability for nonpolar antimicrobial agents underlies extreme susceptibility of pasteurella multocida to the hydrophobic biocide triclosan.pasteurella multocida exhibits nonspecific susceptibility to nonpolar antimicrobial agents such as triclosan, despite possessing an ultrastructurally typical gram-negative cell envelope. capsulated and noncapsulated cell surface variants were examined to investigate the role outer membrane permeability plays in triclosan susceptibility. test strains were unable to initiate growth in the presence of bile salts and were susceptible to triclosan with minimal inhibitory concentrations (mics) ranging ...200717560745
differentiation of avian pasteurella multocida strains by single-primer pcr. 200717294263
isolated endocarditis of the pulmonary valve caused by pasteurella multocida.we describe the rare case of a patient with an isolated endocarditis of the pulmonary valve caused by pasteurella multocida. the bacterium was cultured from blood as well as from the excised valve after pulmonary valve replacement. risk factors were contact with animals and concurrent intravenous drug use.200717297591
[pneumonia with septicemia caused by pasteurella multocida in an immunocompetent patient].pneumonia with septicemia caused by pasteurella multocida was diagnosed in an immunocompetent patient exposed to a dog. this case is remarkable by two aspects: first the absence of visible cutaneous lesion, and second the localization and severity of the infection caused by p. multocida even though the patient was immunocompetent. p. multocida can cause respiratory and systemic infection, and it is a possible diagnosis in case of exposure to animals, even without history of bite or scratch. furt ...200717303362
communicable disease and health protection quarterly review: july to september 2006. from the health protection agency. 200717311831
pasteurella multocida sepsis, due to a scratch from a pet cat, in a post-chemotherapy neutropenic patient with non-hodgkin lymphoma.pasteurella (p) multocida exists in a variety of animals and causes diverse infections in humans due to animal bites and scratches, usually by cats or dogs, and oral and respiratory infection. we report a case of p multocida sepsis due to a scratch from a pet cat, complicated with disseminated intravascular coagulation in a post-chemotherapy neutropenic patient with non-hodgkin lymphoma. the patient was a febrile 79-year-old woman with disturbed consciousness and subcutaneous abscess in her righ ...200717321993
effect of ivermectin on the cellular and humoral immune responses of rabbits.the objective of this paper is to determine the effect of ivermectin administration on cell mediated (cmi) and humoral immunity (hi) of rabbits. cmi against dinitrochlorobenzene (dncb) and sheep red blood cells (srbc) in rabbits was determined by delayed-type hypersensitivity and macrophage engulfment assay (mea), respectively; whereas, hi to pasteurella multocida b2 vaccine and srbc was determined by indirect haemagglutination assay (iha) and jerne hemolytic plaque formation assay (jhpfa), resp ...200717379254
experimental transmission of pasteurella multocida 6:b in goats.haemorrhagic septicaemia (hs) is an acute disease of cattle and buffaloes caused by pasteurella multocida 6:b. outbreaks of the disease have been closely associated with carrier animals that transmit the organism to susceptible animals during stressful condition. this study was conducted to determine whether goats exposed intranasally to p. multocida 6:b can transmit the organism to contact goats. thirty-six healthy local katjang goats were divided into four groups and goats of groups 1 and 3 we ...200717381677
[pasteurella dagmatis: an unusual cause of vertebral osteomyelitis].vertebral osteomyelitis is a quite rare but severe cause of back pain in adults. various causative organisms have been reported. pasteurella species have rarely been isolated. we report here a case of vertebral osteomyelitis and consecutive cauda equina syndrome due to pasteurella dagmatis in a 60-year-old diabetic man.200717391865
reclassification of [pasteurella] trehalosi as bibersteinia trehalosi gen. nov., comb. nov.[pasteurella] trehalosi is an important pathogen of sheep, being primarily associated with serious systemic infections in lambs but also having an association with pneumonia. the aim of the present investigation was to characterize a broad collection of strains tentatively identified as [p.] trehalosi in order to reclassify and rename this taxon to support improvements in our understanding of the pathogenesis and epidemiology of this important organism. the type strain for [p.] trehalosi, strain ...200717392184
[bacteriological and virological status in upper respiratory tract infections of cats (cat common cold complex)].between october 2002 and january 2005,460 bacteriological samples from cats with an acute upper respiratory tract infection were analysed in clinical field studies in two accredited laboratories in germany. oropharyngeal swabs were taken from these cats and sent to the laboratories for routine diagnostics. in the swab samples of 460 cats 382 bacteria strains were isolated. the following bacteria were isolated most frequently: pasteurella spp. (32.5 %), staphylococcus spp. (18.5 %), escherichia c ...200717416134
ultrastructural characteristics of the external surfaces of pasteurella pneumotropica from mice and pasteurella multocida from rabbits.the surface structures of the cells of pasteurella pneumotropica from mice and pasteurella multocida from rabbits were examined by transmission electron microscopy after ruthenium red staining and polycationic ferritin labelling. p. pneumotropica strains atcc 35149 and k 79114 had slight extracellular fibrous materials associated with cell walls with ruthenium red staining. ferritin labelling method revealed thick strands or sparsely ferritin-labelled materials on the cell surface of the strains ...200717430628
the tad locus: postcards from the widespread colonization island.the tad (tight adherence) macromolecular transport system, which is present in many bacterial and archaeal species, represents an ancient and major new subtype of type ii secretion. the tad genes are present on a genomic island named the widespread colonization island (wci), and encode the machinery that is required for the assembly of adhesive flp (fimbrial low-molecular-weight protein) pili. the tad genes are essential for biofilm formation, colonization and pathogenesis in the genera aggregat ...200717435791
pasteurella meningo-encephalitis--a risk of household pets. 200717442396
protective immunity conferred by recombinant pasteurella multocida lipoprotein e (plpe).the genes encoding pasteurella multocida lipoprotein e (plpe) and lipoprotein b (plpb) were cloned from p. multocida strain x-73 (serotype a:1) and expressed in escherichia coli. the protective immunity conferred by recombinant plpe (r-plpe) and plpb (r-plpb) on mice and chickens was evaluated. the results showed that mice immunized with 10microg of purified r-plpe were protected (80-100% survival rate) against challenge infection with 10 or 20 ld(50) of p. multocida strains x-73 (serotype a:1), ...200717449151
successful treatment of cervical spinal epidural empyema secondary to grass awn migration in a cat.spinal epidural empyema (see) represents a severe pyogenic infection of the epidural space. clinical signs of the disease are non-specific--increased body temperature, intense neck pain, neurological signs of a transverse myelopathy--and can lead to severe and permanent neurological deficits. this report describes the diagnosis and successful surgical treatment of cervical see secondary to grass awn migration in a cat. although it is uncommon, this disease should be suspected in cats with progre ...200717449314
comparative sequence analysis of 16s rrna gene of pasteurella multocida serogroup b isolates from different animal species.the phylogenetic relationships of five isolates of pasteurella multocida serotype b:2 belonging to buffalo, cattle, pig, sheep and goat were investigated by comparative sequence analysis of 16s rrna gene. the 1468bp fragment of 16s rrna gene sequence comparison showed that the isolates of cattle (pm75), pig (pm49) and sheep (pm82) shared 99.9% homology with the buffalo isolate (vaccine strain p52) whereas, the goat isolate (pm86) shared 99.8% homology with the vaccine strain. the 16s rrna gene s ...200717459437
outer membrane protein h for protective immunity against pasteurella multocida.pasteurella multocida, a gram-negative facultative anaerobic bacterium, is a causative animal pathogen in porcine atrophic rhinitis and avian fowl cholera. for the development of recombinant subunit vaccine against p. multocida, we cloned and analyzed the gene for outer membrane protein h (omph) from a native strain of pasteurella multocida in korea. the omph had significant similarity in both primary and secondary structure with those of other serotypes. the full-length, and three short fragmen ...200717483806
crystal structures of pasteurella multocida sialyltransferase complexes with acceptor and donor analogues reveal substrate binding sites and catalytic mechanism.sialyltransferases are key enzymes involved in the biosynthesis of biologically and pathologically important sialic acid-containing molecules in nature. binary x-ray crystal structures of a multifunctional pasteurella multocida sialyltransferase (delta24pmst1) with a donor analogue cmp-3f(a)neu5ac or cmp-3f(e)neu5ac were determined at 2.0 and 1.9 a resolutions, respectively. ternary x-ray structures of the protein in complex with cmp or a donor analogue cmp-3f(a)neu5ac and an acceptor lactose ha ...200717487984
protostrongylid parasites and pneumonia in captive and wild thinhorn sheep (ovis dalli).we describe health significance of protostrongylid parasites (parelaphostrongylus odocoilei and protostrongylus stilesi) and other respiratory pathogens in more than 50 naturally infected dall's sheep (ovis dalli dalli) from the mackenzie mountains, northwest territories (1998-2002) as well as in three stone's sheep (o. d. stonei) experimentally infected with p. odocoilei (2000-2002). histological lesions in the brain and distribution of p. odocoilei in the muscles of experimentally and naturall ...200717495303
pasteurella multocida expresses two lipopolysaccharide glycoforms simultaneously, but only a single form is required for virulence: identification of two acceptor-specific heptosyl i transferases.lipopolysaccharide (lps) is a critical virulence determinant in pasteurella multocida and a major antigen responsible for host protective immunity. in other mucosal pathogens, variation in lps or lipooligosaccharide structure typically occurs in the outer core oligosaccharide regions due to phase variation. p. multocida elaborates a conserved oligosaccharide extension attached to two different, simultaneously expressed inner core structures, one containing a single phosphorylated 3-deoxy-d-manno ...200717517879
characterization of a broad range antibacterial substance from a new bacillus species isolated from amazon basin.a bacillus sp. strain producing a bacteriocin-like substance was characterized by biochemical profiling and 16s rdna sequencing. the phylogenetic analysis indicated that this strain has low sequence similarity with most bacillus spp., suggesting a new species was isolated. the antimicrobial activity was detected starting at the exponential growth phase, and maximum activity was observed at stationary phase. the substance was inhibitory to a broad range of indicator strains, including pathogenic ...200717534601
a specific polymerase chain reaction based on the gyrb gene sequence and subsequent restriction fragment length polymorphism analysis of pasteurella pneumotropica isolates from laboratory mice.for a molecular identification and typing tool, we developed a specific polymerase chain reaction (pcr) based on the gyrb gene sequence and a subsequent restriction fragment length polymorphism (rflp) analysis using the products amplified from the specific pcr to facilitate discrimination of biotypes of pasteurella pneumotropica from laboratory mice. appropriate pcr products, a 1039-basepair fragment for biotype jawetz and a 1033-basepair fragment for biotype heyl, were amplified by use of the p ...200717343354
pasteurella multocida detection by 5' taq nuclease assay: a new tool for use in diagnosing fowl cholera.a 5' taq nuclease assay utilising minor groove binder technology and targeting the 16s rrna gene was designed to detect pasteurella multocida (the causative agent of fowl cholera) in swabs collected from poultry. the assay was first evaluated using pure cultures. the assay correctly identified four p. multocida taxonomic type strains, 18 p. multocida serovar reference strains and 40 australian field isolates (17 from poultry, 11 from pigs and 12 from cattle). representatives of nine other pasteu ...200717346833
mannheimia (pasteurella) haemolytica leukotoxin utilizes cd18 as its receptor on bighorn sheep leukocytes.pneumonia caused by mannheimia (pasteurella) haemolytica is a highly fatal disease of bighorn sheep (ovis canadensis). leukotoxin (lkt), secreted by m. haemolytica, is an important virulence factor of this organism, and is cytolytic to bighorn sheep leukocytes. previously, we have shown that cd18, the beta subunit of beta2 integrins, serves as the receptor for lkt on bovine leukocytes. furthermore, anti-cd18 antibodies inhibit lkt-induced cytotoxicity of bighorn sheep leukocytes. therefore, we h ...200717347395
specific identification of gallibacterium by a pcr using primers targeting the 16s rrna and 23s rrna genes.gallibacterium was recently established as a new genus including organisms previously reported as pasteurella anatis, [actinobacillus] salpingitidis and avian pasteurella haemolytica-like organisms. the aim of the present study was to develop a pcr method allowing unambiguous identification of gallibacterium. pcr primers positioned in the 16s rrna (1133fgal) and 23s rrna (114r) genes were defined and their specificity was subsequently tested on 122 strains. twenty-five of the strains represented ...200717350770
crystal structures reveal a thiol protease-like catalytic triad in the c-terminal region of pasteurella multocida toxin.pasteurella multocida toxin (pmt), one of the virulence factors produced by the bacteria, exerts its toxicity by up-regulating various signaling cascades downstream of the heterotrimeric gtpases gq and g12/13 in an unknown fashion. here, we present the crystal structure of the c-terminal region (residues 575-1,285) of pmt, which carries an intracellularly active moiety. the overall structure of c-terminal region of pmt displays a trojan horse-like shape, composed of three domains with a "feet"-, ...200717360394
successful treatment of pasteurella multocida meningitis with aztreonam.this is the first reported case of the successful treatment of pasteurella multocida meningitis with aztreonam in a patient with multiple antibiotic allergies.200717366017
fatal pasteurella multocida septicemia and necrotizing fasciitis related with wound licked by a domestic dog.a 68-y-old male had necrotizing fasciitis and bacteremia due to pasteurella multocida. saliva culture from his dog grew p. multocida and pseudomonas aeruginosa. the human and dog p. multocida strains were of the same antibiogram but not identical tested with ribotyping. the wound licked by his dog was the only risk factor.200717366037
mannheimia haemolytica leukotoxin-induced cytolysis of ovine (ovis aries) leukocytes is mediated by cd18, the beta subunit of beta2-integrins.mannheimia (pasteurella) haemolytica causes severe pneumonia in cattle, sheep and goats. leukotoxin (lkt) is the most important virulence determinant produced by this organism. previously, we identified cd18, the beta subunit of beta(2)-integrins, as the receptor for lkt on bovine leukocytes. since lkt is specific for leukocytes of cattle, sheep and goats, we hypothesized that lkt utilizes cd18 as its receptor on ovine leukocytes as well. therefore, the objective of this study was to transfect a ...200717368825
mutations in 16s rrna and ribosomal protein s5 associated with high-level spectinomycin resistance in pasteurella multocida.pasteurella multocida isolates with high-level spectinomycin resistance in which no adenyltransferase genes could be demonstrated exhibited a c1192g transversion in the 16s rrna of all six or five of the six rrna operons and/or two different types of 3-bp deletions in the rpse gene that codes for the ribosomal protein s5.200717371823
modulation of host cell gene expression through activation of stat transcription factors by pasteurella multocida toxin.the pasteurella multocida toxin (pmt) is highly mitogenic and has potential carcinogenic properties. pmt causes porcine atrophic rhinitis that is characterized by bone resorption and loss of nasal turbinates, but experimental nasal infection also leads to excess proliferation of bladder epithelial cells. pmt acts intracellularly and activates phospholipase c-linked signals and mapk pathways via the heterotrimeric galpha(q) and galpha(12/13) proteins. we found that pmt induces activation of stat ...200717150962
cloning and characterization of type 4 fimbrial gene (ptfa) of pasteurella multocida serogroup b:2 (strain p52). 200717216317
cpg oligodeoxynucleotides augment the immune responses of piglets to swine pasteurella multocida living vaccine in vivo.oligodeoxynucleotides containing unmethylated cpg motifs (cpg odn) prevent development of t-helper type 2 (th2) immune response and reverse established allergic responses in mouse models. however, little work on immune responses in piglets has been conducted in vivo. in this report, the ability of a porcine-specific cpg odn to act as an immunostimulant and enhance immune responses of piglets to swine pasteurella multocida living vaccine (spml vaccine) was determined. the titre of igg and igg1/ig ...200717223146
efficacy of haemorrhagic septicaemia alum-precipitated vaccine. 200717277309
etiology of respiratory disease in non-vaccinated, non-medicated calves in rearing herds.the aim of this study was to examine the occurrence of bacterial, mycoplasmal and viral pathogens in the lower respiratory tract of calves in all-in all-out calf-rearing units. according to clinical status, non-medicated calves with and without respiratory disease signs were selected of the 40 herds investigated to analyse the micro-organisms present in healthy and diseased calves. tracheobronchial lavage (tbl) and paired serum samples were analysed for bacteria, mycoplasmas, respiratory syncyti ...200717084565
synthesis and biological study of medicinally important mannich bases derived from 4-(dimethylamino)-1,4,4a,5,5a,6,11,12a-octahydro-3,6,10,12,12a pentahydroxy naphthacene carboxamide.the paper describes synthesis and antibacterial study of biologically active mannich bases of carboxamide derivative employing mannich reaction of 4-(dimethylamino)-1,4,4a,5,5a,6,11,12a-octahydro-3,6,10,12,12a pentahydroxy naphthacene carboxamide with various sulfonamides/secondary amines . they were analysed by elemental analysis and characterized by uv, ir and (1)h nmr spectroscopic studies. the mannich bases were screened for antibacterial activity against various gram-negative bacteria at va ...200717110110
cloning of the gene and characterization of the enzymatic properties of the monomeric alkaline phosphatase (phox) from pasteurella multocida strain x-73.we have identified a new phox gene encoding the monomeric alkaline phosphatase from pasteurella multocida x-73. this gene was not found in the published genome sequence of pasteurella multocida pm70. characterization of the recombinant phox of pasteurella multocida x-73 showed that it is a monomeric enzyme, activated by ca(2+) and possibly secreted by the tat pathway. these features distinguish phosphatases of the phox family from those of the phoa family. all proteins of the phox family were fo ...200717156125
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