Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| antibody to haemophilus ducreyi among trucking company workers in kenya. | to determine the prevalence, correlates, and incidence of haemophilus ducreyi antibodies, a cohort of east african trucking company employees was evaluated. | 1997 | 9153735 |
| dermatology and the recently returned traveler: infectious diseases with dermatologic manifestations. | 1997 | 9158995 | |
| alterations in levels of dnak and groel result in diminished survival and adherence of stressed haemophilus ducreyi. | haemophilus ducreyi is a hemin-requiring bacterium causing the genital ulcer disease chancroid. previously we demonstrated that the heat shock protein groel was immunogenic and possibly highly expressed in a mammalian host. the present study was initiated to (i) determine the relative amounts of groel expressed by h. ducreyi during in vitro exposure to stresses and (ii) evaluate whether a high level of groel is directly or indirectly required for survival and adherence of stressed h. ducreyi. us ... | 1997 | 9169782 |
| comparison of enzyme immunoassays for antibodies to haemophilus ducreyi in a community outbreak of chancroid in the united states. | the performance of two eias (adsorption eia and lipooligosaccharide [los] eia) that detect antibodies to haemophilus ducreyi was evaluated with serum specimens obtained from 163 patients (96 with genital ulcer disease [gud]). paired serum specimens (initial and follow-up) were obtained from 52 of the gud patients. by use of initial serum specimens from 82 gud patients whose etiologic agents for their ulcers had been identified, the adsorption eia had a sensitivity and specificity for chancroid o ... | 1997 | 9180178 |
| iron-regulated haemolysin gene from edwardsiella tarda. | we have cloned and sequenced the haemolysin gene locus from edwardsiella tarda (eth). this region encoded two open reading frames, designated etha and ethb. etha is the haemolysin gene consisting of 4782bp encoding a product of 165.3 kda and ethb is an activation/secretion protein gene of 1677bp that encodes a product of 61.9 kda. there were two putative ferric uptake regulator (fur) binding sites on the 5' upstream region of the ethb gene overlapping the promoter region and ribosome-binding sit ... | 1997 | 9194711 |
| structurally defined epitopes of haemophilus ducreyi lipooligosaccharides recognized by monoclonal antibodies. | by use of enzyme-linked immunosorbent assay and immunoblotting techniques, the migration patterns and binding epitopes of lipooligosaccharides (los) from 10 haemophilus ducreyi strains were investigated with two monoclonal antibodies (mabs), mahd6 and mahd7, raised against los from h. ducreyi itm 2665. closely related los, with defined structures, from haemophilus influenzae, bordetella pertussis, aeromonas spp., and synthetic glycoproteins were also included in the analyses. the mabs bound to c ... | 1997 | 9234768 |
| purification and identification of haemophilus ducreyi cytotoxin by use of a neutralizing monoclonal antibody. | haemophilus ducreyi produces a cytotoxin responsible for the killing of cultured human epithelial cells. cytotoxin-neutralizing antibodies were detected in the majority of sera from patients with culture-proven chancroid, and a significantly higher level of such antibodies in patients than in blood donors was noted both in areas where the disease is endemic and those where it is not. we produced neutralizing monoclonal antibodies (mabs) in mice with a crude osmotic preparation of the cytotoxin. ... | 1997 | 9234821 |
| characterization of a transposon tn916-generated mutant of haemophilus ducreyi 35000 defective in lipooligosaccharide biosynthesis. | to define the role of the surface lipooligosaccharide (los) of haemophilus ducreyi in the pathogenesis of chancroid, tn916 mutants of h. ducreyi 35000 defective in expression of the murine monoclonal antibody (mab) 3f11 epitope on h. ducreyi los were identified by immunologic screening. one mutant, designated 1381, has an los which lacks the mab 3f11 epitope and migrates with an increased mobility on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. the gene disrupted by the tn916 eleme ... | 1997 | 9260947 |
| immunohistochemical investigations of genital ulcers caused by haemophilus ducreyi. | to gain information on the specific composition of the inflammatory infiltrate of genital ulcers caused by haemophilus ducreyi, biopsies of 6 genital ulcers which were diagnosed as chancroid on clinical and microbiological grounds were subjected to immunohistochemical investigations after conventional haematoxylineosin staining. a variety of antibodies reactive against b- and t-cells, plasma cells and granulocytes were used with each tissue sections. the lymphocytic infiltrate of chancroid ulcer ... | 1997 | 9292349 |
| the association of herpes simplex virus type 2 (hsv-2), haemophilus ducreyi, and syphilis with hiv infection in young men in northern thailand. | to evaluate the association between sexually transmitted diseases that commonly may cause genital ulceration and prevalent and incident hiv infections, we conducted three case control studies in a cohort of 21-year-old male military conscripts in northern thailand. the men were evaluated at baseline in 1991 and semiannually until their discharge 2 years later. serologic evidence of infection with herpes simplex virus type 2 (hsv-2), haemophilus ducreyi, and hiv were more frequent at baseline in ... | 1997 | 9402077 |
| sexually transmitted diseases and risk of hiv infection in men attending a sexually transmitted diseases clinic in dakar, senegal. | this cross-sectional study was carried out among male outpatients with symptoms of stds at the std reference centre at the institute of social hygiene (ihs), dakar, senegal, from march 1989 through may 1991. this study was used to determine the prevalence of stds and hiv among male patients attending an std clinic and to identify their socio-demographic characteristics and risk factors. a total of 975 patients were enrolled in the study. the most common syndromes were urethritis (76%) and genita ... | 1997 | 10214412 |
| the laboratory of reproductive tract infections. | the laboratory of reproductive tract infections (rtis) is a new entity within the laboratory sciences division of the icddr,b. the objectives of the laboratory are to conduct epidemiologic research in rtis, including sexually transmitted diseases (stds), to provide technical support to protocols developed in other scientific divisions of the center, to provide training in diagnostic procedures, and to give diagnostic support to patient care. the rti laboratory has an extremely important role i ... | 1997 | 12293269 |
| sexually transmitted diseases: a survey of case management in malawi. | a national survey of sexually transmitted disease (std) case management was carried out at 39 health care facilities in malawi in 1994. fifty-four health care providers were observed managing 150 patients presenting with selected std syndromes and 103 providers were interviewed. std case management was assessed by calculation of who/gpa prevention indicators (pis) from observation data. the overall rate for pi-6, which measures correct assessment and treatment of std patients was 11% (81% for hi ... | 1996 | 8876358 |
| increased risk of infection with human immunodeficiency virus type 1 among uncircumcised men presenting with genital ulcer disease in kenya. | the factors responsible for the explosive spread of human immunodeficiency virus type 1 (hiv-1) in sub-saharan africa continue to be identified and debated. one of the most controversial factors has been male circumcision. this cross-sectional study was conducted to measure the association between circumcision status and infection with hiv-1 among men with genital ulcer disease. eight hundred and ten men participated in the study, of whom 190 (23%) were hiv-1-positive. a logistic regression mode ... | 1996 | 8879763 |
| ribosomal dna typing of haemophilus ducreyi strains: proposal for a novel typing scheme. | local strains of haemophilus ducreyi were characterized by ribosomal dna fingerprinting in order to develop a typing scheme for this organism. chromosomal dnas from 30 strains were extracted, and restriction fragments were separated by agarose gel electrophoresis after cleavage with bglii, hindiii, hindii, and bsteii. of the 30 local strains studied, nine bglii, eight hindiii, six hindii, and five bsteii ribotype patterns were observed. in order to develop a typing scheme, we correlated the ribo ... | 1996 | 8880534 |
| a morphological study of penile chancroid lesions in human immunodeficiency virus (hiv)-positive and -negative african men with a hypothesis concerning the role of chancroid in hiv transmission. | chancroid, the most common cause of genital ulceration in africa, is known to be associated epidemiologically with heterosexual transmission of human immunodeficiency virus (hiv). the pathophysiological mechanisms by which chancroid might facilitate the spread of hiv are obscure. to investigate the role of chancroid in hiv transmission, the authors studied the histological features of biopsies from 11 men with penile chancroid lesions including five who were serologically positive for hiv. the h ... | 1996 | 8892592 |
| std care in zambia: an evaluation of the guidelines for case management through a syndromic approach. | clinical diagnosis of stds is unreliable and therefore constitutes a poor basis for choice of treatment. a syndromic approach has been suggested to increase effectiveness of treatment in resource poor settings. algorithms for the treatment of std syndromes were evaluated. a total of 436 patients were followed; cure rates were defined and estimated for genital ulcer disease (gud), urethral and vaginal discharge. cure rates for the discharge syndromes were high, 97-98%, for both sexes. the cure ra ... | 1996 | 8894821 |
| cloning and sequencing of a haemophilus ducreyi fur homolog. | iron regulation in a growing number of bacterial species is being attributed to the presence of a fur (ferric uptake regulation) regulatory system. in the presence of iron, fur acts as a classical negative regulator, binding conserved sequences within the promoter of iron-repressible genes and blocking transcription. western blot analysis utilizing escherichia coli fur antisera detected a band of approximately 17 kda in soluble extracts of haemophilus ducreyi. additionally, southern blot hybridi ... | 1996 | 8918243 |
| a history of the detection of haemophilus ducreyi, 1889-1979. | 1996 | 8919733 | |
| monoclonal antibodies against haemophilus influenzae lipopolysaccharides: clone mahi 4 binding to a pentasaccharide containing terminal beta-gal residues and clone mahi 10 recognizing terminal phosphorylated saccharide residues. | mouse monoclonal antibodies mahi 4 and mahi 10 reactive with haemophilus influenzae lipopolysaccharide (lps), were generated by fusing mouse myeloma cells with spleen cells of mice immunized with h. influenzae strain rm.7004-xp-1. the antibody mahi 4 reacted in whole-cell enzyme immunoassay (eia) and colony-dot-immunoblotting with 20 of 123 h. influenzae strains and to a few other human haemophilus spp. and neisseria spp., but not to any bordetella pertussis, b. parapertussis, aeromonas spp. or ... | 1996 | 8938639 |
| characterization of a novel lipoprotein expressed by haemophilus ducreyi. | pooled sera from patients with chancroid contain antibodies to a haemophilus ducreyi antigen with an approximate molecular weight of 28,000 (28k). rabbit polyclonal serum that reacts to a 28k protein can be used to detect h. ducreyi in clinical samples. a monoclonal antibody, designated 5c9, bound to a 28k outer membrane protein and to 35 of 35 h. ducreyi isolates with diverse geographic origins and did not bind to many species of the families pasteurellaceae, neisseriaceae, and enterobacteriace ... | 1996 | 8945545 |
| comparison of the etest with agar dilution for antimicrobial susceptibility testing of haemophilus ducreyi. | two methods for determining the sensitivity of haemophilus ducreyi to antimicrobial agents were compared; an agar dilution method and the etest. the mics of seven antimicrobial agents; penicillin v, ampicillin, trimethoprim, tetracycline, chloramphenicol, erythromycin and ciprofloxacin were determined for 20 strains of h. ducreyi. the mics determined with the etest and with the agar dilution method, showed 86% agreement within one two-fold dilution. the etest is a good alternative method to the ... | 1996 | 8961054 |
| cloning and sequencing of the gene encoding the cu,zn-superoxide dismutase of haemophilus ducreyi. | the sodc gene of haemophilus ducreyi was cloned and sequenced. the deduced amino acid sequence of this protein exhibited 71.6% identity and 81.8% similarity to the h. influenzae and h. parainfluenzae copper (cu), zinc (zn)-superoxide dismutase (sod) enzymes. this gene was localized to a 2.2-kb h. ducreyi chromosomal dna insert in plasmid phdsod. sod activity was expressed in cell-free extracts of escherichia coli containing the recombinant plasmid phdsod and was localized to the periplasmic spac ... | 1996 | 8996084 |
| the lipooligosaccharides of haemophilus ducreyi are highly sialylated. | the major lipooligosaccharides of the sexually transmitted pathogen haemophilus ducreyi 35000 have been previously found to terminate in n-acetyllactosamine and sialyl-n-acetyllactosamine, neu5ac alpha 2-->3gal beta 1-->4glcnac (w. melaugh, n. j. phillips, a. a. campagnari, m. v. tullius, and b. w. gibson, biochemistry 33: 13070-13078, 1994). in this study, mass spectrometry and composition analyses have shown that the lipooligosaccharides from three other h. ducreyi strains also contain n-acety ... | 1996 | 8550483 |
| fine tangled pili expressed by haemophilus ducreyi are a novel class of pili. | haemophilus ducreyi synthesizes fine, tangled pili composed predominantly of a protein whose apparent molecular weight is 24,000 (24k). a hybridoma, 2d8, produced a monoclonal antibody (mab) that bound to a 24k protein in h. ducreyi strains isolated from diverse geographic locations. a lambda gt11 h. ducreyi library was screened with mab 2d8. a 3.5-kb chromosomal insert from one reactive plaque was amplified and ligated into the pcrii vector. the recombinant plasmid, designated phd24, expressed ... | 1996 | 8550517 |
| a hemoglobin-binding outer membrane protein is involved in virulence expression by haemophilus ducreyi in an animal model. | haemophilus ducreyi exhibits a requirement for exogenously supplied heme for aerobic growth in vitro. nine of ten wild-type isolates of h. ducreyi were shown to contain a readily detectable hemoglobin-binding activity. spontaneous hemoglobin-binding-negative mutants of two of these wild-type isolates lost the ability to express an outer membrane protein with an apparent molecular mass of approximately 100 kda. similarly, the single wild-type isolate that lacked the ability to bind hemoglobin als ... | 1996 | 8613384 |
| evaluation of humoral and cell-mediated inducible immunity to haemophilus ducreyi in an animal model of chancroid. | to study the mechanisms of inducible immunity to haemophilus ducreyi infection in the temperature-dependent rabbit model of chancroid, we conducted passive immunization experiments and characterized the inflammatory infiltrate of chancroidal lesions. polyclonal immunoglobulin g was purified from immune sera raised against h. ducreyi 35000 whole-cell lysate or a pilus preparation and from naive control rabbits. rabbits were passively immunized with 24 or 48 mg of purified polyclonal immunoglobuli ... | 1996 | 8613391 |
| treatment of chancroid with azithromycin. | a randomized, comparative study undertaken in nairobi, kenya and a non-comparative evaluation undertaken in carletonville, south africa have both shown that a single oral dose of azithromycin 1 g is effective in the treatment of the genital ulcer disease (gud), chancroid, with cure rates of 89% and 92% recorded respectively. while treatment failure was associated with human immunodeficiency virus seropositivity and lack of circumcision in kenya, no such association could be found in the south af ... | 1996 | 8652728 |
| purification, cloning, and expression of a cytidine 5'-monophosphate n-acetylneuraminic acid synthetase from haemophilus ducreyi. | an n-acetylneuraminic acid cytidylyltransferase (ec 2.7.7.43) (cmp-neuac synthetase) was isolated from a haemophilus ducreyi strain 35000 cell lysate and partially characterized. the enzyme catalyzes the reaction of ctp and neuac to form cmp-neuac, which is the nucleotide sugar donor used by sialyltransferases. previous studies have shown that the outer membrane lipooligosaccharides of h. ducreyi contain terminal sialic acid attached to n-acetyllactosamine and that this modification is likely im ... | 1996 | 8663048 |
| the conserved 18,000-molecular-weight outer membrane protein of haemophilus ducreyi has homology to pal. | haemophilus ducreyi expresses an 18,000-molecular-weight outer membrane protein that contains a conserved surface-exposed epitope recognized by monoclonal antibody 3b9. monoclonal antibody 3b9 cross-reacts with proteins of similar molecular weight found in many haemophilus sp. strains, including p6, a candidate vaccine for haemophilus influenzae. the gene encoding the 18,000-molecular-weight outer membrane protein was identified by screening a lambdagt11 genomic library with 3b9. the coding sequ ... | 1996 | 8675292 |
| haemophilus ducreyi hemolysin acts as a contact cytotoxin and damages human foreskin fibroblasts in cell culture. | haemophilus ducreyi, which causes the sexually transmitted disease chancroid, produces several factors that damage human cells. we used isogenic mutants of h. ducreyi 35000 to demonstrate that the hemolytic activity and the cytotoxic effect of h. ducreyi on human foreskin fibroblasts are due to the same toxin. | 1996 | 8675349 |
| an immunohistochemical analysis of naturally occurring chancroid. | haemophilus ducreyi is a major cause of genital ulcer disease in many developing countries and is associated with augmented transmission of human immunodeficiency virus (hiv). however, the mechanisms through which h. ducreyi produces ulceration are poorly understood. the characteristics of the host response to h. ducreyi and the pathobiology of its potential contribution to increased hiv susceptibility are not known. chancroid ulcer biopsies from 8 patients were analyzed histologically and immun ... | 1996 | 8699082 |
| chancroid. | 1996 | 8743249 | |
| simultaneous pcr detection of haemophilus ducreyi, treponema pallidum, and herpes simplex virus types 1 and 2 from genital ulcers. | a multiplex pcr (m-pcr) assay with colorimetric detection was devised for the simultaneous amplification of dna targets from haemophilus ducreyi, treponema pallidum, and herpes simplex virus (hsv) types 1 and 2. by using target-specific oligonucleotides in a microwell format, 298 genital ulcer swab specimens collected in new orleans during three intervals from 1992 through 1994 were evaluated. the results of the m-pcr assay were compared with the results of dark-field microscopy and h. ducreyi c ... | 1996 | 8748271 |
| identification of an outer membrane protein involved in utilization of hemoglobin-haptoglobin complexes by nontypeable haemophilus influenzae. | a recombinant plasmid containing a 6.5-kb fragment of nontypeable haemophilus influenzae (nthi) chromosomal dna was shown to confer a hemoglobin-haptoglobin-binding phenotype on escherichia coli. use of a mini-tn10kan transposon for random insertion mutagenesis of this recombinant plasmid allowed localization of the nthi dna responsible for this hemoglobin-haptoglobin-binding phenotype to a 3.5-kb psti-xhoi fragment within the 6.5-kb nthi dna insert. when this mutagenized nthi dna fragment was u ... | 1996 | 8751920 |
| cloning of a dna fragment encoding a heme-repressible hemoglobin-binding outer membrane protein from haemophilus influenzae. | haemophilus influenzae is able to use hemoglobin as a sole source of heme, and heme-repressible hemoglobin binding to the cell surface has been demonstrated. using an affinity purification methodology, a hemoglobin-binding protein of approximately 120 kda was isolated from h. influenzae type b strain hi689 grown in heme-restricted but not in heme-replete conditions. the isolated protein was subjected to n-terminal amino acid sequencing, and the derived amino acid sequence was used to design corr ... | 1996 | 8757844 |
| [sexually transmitted diseases in men]. | sexually transmitted diseases (std) constitute a frequent presenting complaint. the epidemiology of human immunodeficiency virus (hiv) infection is identical to that of std and must therefore be systematically investigated in the presence of any std. chlamydia trachomatis (ct) is involved in the majority of cases of urethritis and epididymitis in young subjects and is present in the urethra of 10% of subjects with a genital ulcer. genital ulcers are due to either treponema pallidum, haemophilus ... | 1996 | 8763705 |
| antimicrobial activity of rifabutin. | rifabutin is a spiro-piperidyl-rifamycin derived from rifamycin-s. it is structurally related to rifampin and shares many of its properties. rifabutin has a broad spectrum of antimicrobial activity. it is considerably more active than rifampin in vitro against the mycobacterium avium complex (mac), mycobacterium tuberculosis, and mycobacterium leprae. it also is active against most atypical mycobacteria, including mycobacterium kansasii, but mycobacterium chelonae is relatively resistant. rifabu ... | 1996 | 8785253 |
| presumptive specific clinical diagnosis of genital ulcer disease (gud) in a primary health care setting in nairobi. | of 22,274 patients 12 years of age or older attending a primary health care clinic in nairobi, 1076 (4.8%) complained of symptoms suggesting a sexually transmitted disease (std). of these, 518 females and 462 males underwent complete clinical evaluation, and 78% had objective microbiologic or clinical evidence of std, including 168 (17.1%) with genital ulcer disease (gud). presumptive specific clinical diagnoses on initial physical examination in cases of gud were chancroid (131 patients), syphi ... | 1996 | 8799783 |
| haemophilus ducreyi elicits a cutaneous infiltrate of cd4 cells during experimental human infection. | human subjects were experimentally infected with haemophilus ducreyi for up to 2 weeks. bacterial suspensions were delivered into the epidermis and dermis through puncture wounds made by an allergy-testing device. subjects developed papular lesions that evolved into pustules resembling natural disease. some papular lesions resolved spontaneously, indicating that host responses may clear infection. bacteria were shed intermittently from lesions, suggesting that h. ducreyi may be transmissible bef ... | 1996 | 8568301 |
| organization of the haemophilus ducreyi 35000 chromosome. | a physical and rudimentary genetic map of the haemophilus ducreyi strain 35000 genome was constructed. pulsed-field gel electrophoresis was used to separate restriction fragments of h. ducreyi dna digested with sfil, i-ceul, or sfil plus i-ceul. the sizes of the fragments were determined, and the circular chromosome was estimated to be 1757 kbp. the six i-ceul fragments and four sfil fragments were ordered into macrorestriction maps using southern blot hybridization with random h. ducreyi clones ... | 1996 | 8828227 |
| a prospective clinical and bacteriologic study of inguinal buboes in thai men. | one-hundred thirteen men (mean age, 23 years) who presented with inguinal buboes to a government-operated hospital for sexually transmitted diseases (stds) in bangkok were studied between february 1987 and february 1989. the median duration of preceding symptoms was 7 days (range, 1-62 days). the majority of patients (74; 65%) had received treatment previously; 31 (27%) were febrile, 13 (12%) had extrainguinal lymphadenopathy, and 31 (27%) had concurrent active genital ulcers. there was no histo ... | 1996 | 8838178 |
| an isogenic haemolysin-deficient mutant of haemophilus ducreyi lacks the ability to produce cytopathic effects on human foreskin fibroblasts. | the haemolysin of haemophilus ducreyi is the newest member of the proteus/serratia family of pore-forming toxins. in order to assess the role of the haemolysin in virulence, we constructed an isogenic haemolysin-deficient mutant of h. ducreyi strain 35000 this strain, designated 35000-3, lacks detectable haemolytic activity. we tested h. ducreyi strains 35000 and 35000-3 for their cytopathic activity against human foreskin fibroblasts (hffs). we observed strong cytopathic activity when strain 35 ... | 1996 | 8843430 |
| medical microbiology | genitourinary infections fall into two main categories: (1) primary infections due to sexually transmitted pathogenic microorganisms and (2) infections due to members of the resident flora. genital infections are uncommon in children and increase dramatically in sexually active adults, in whom sexually transmitted diseases are the second most prevalent group of reportable communicable illness in north america. sexual ... | 1996 | 21413302 |
| medical microbiology | the genus haemophilus includes a number of species that cause a wide variety of infections but share a common morphology and a requirement for blood-derived factors during growth that has given the genus its name. haemophilus influenzae, the major pathogen, can be separated into encapsulated or typable strains, of which there are seven types (a through f including e') based on the antigenic structure of the capsular polysaccharide, and unencapsulated or nontypable strains. type b h influenzae is ... | 1996 | 21413340 |
| laboratory diagnosis of chancroid using species-specific primers from haemophilus ducreyi groel and the polymerase chain reaction. | to enhance laboratory identification of haemophilus ducreyi, the causative agent of the genital ulcer disease chancroid, a polymerase chain reaction (pcr) assay was developed using target dna sequences from the essential h. ducreyi gene, groel. positive reactions were obtained in this pcr assay with 139 isolates of h. ducreyi from patients in worldwide locations from the 1940s to the 1990s. in contrast, 24 other bacterial species were negative. when genital ulcer specimens from 162 african patie ... | 1995 | 8849652 |
| chancroid detected by polymerase chain reaction--jackson, mississippi, 1994-1995. | 1995 | 8578414 | |
| enhanced recovery of haemophilus ducreyi from clinical specimens by incubation at 33 versus 35 degrees c. | isolation rates of haemophilus ducreyi from cases of chancroid are low. experts recommend that isolation media be incubated at 33 to 35 degrees c, but the possible effect of this temperature range on the recovery of h. ducreyi has not been evaluated. we inoculated two sets of agar plates with material from genital ulcers and incubated one set at 33 degrees c and one at 35 degrees c; incubation at 33 degrees c identified 21% more cases than did incubation at 35 degrees c (109 versus 85 cases, res ... | 1995 | 8586712 |
| monoclonal antibodies against haemophilus ducreyi lipooligosaccharide and their diagnostic usefulness. | monoclonal antibodies against the lipooligosaccharide of haemophilus ducreyi were produced. two of them, mahd6 and mahd7, were found to be relatively, although not absolutely, specific and reacted with nearly all strains of haemophilus ducreyi tested: 59 of 60 and 60 of 60, respectively. the diagnostic usefulness of mahd7 was assessed. clinical specimens collected in zambia from patients with genital ulcers were tested using indirect immunofluorescence (if), enzyme immunoassay (eia), the polymer ... | 1995 | 8605903 |
| cloning and characterization of the genes encoding the hemolysin of haemophilus ducreyi. | we previously identified a heat- and protease-labile haemolytic activity expressed by haemophilus ducreyi. in order to characterize the haemolysin at the molecular level, genomic dna from h. ducreyi was probed with haemolysin genes from other gram-negative organisms. the haemolysin genes of proteus mirabilis hybridized to h. ducreyi dna suggesting that the haemolysin of h. ducreyi is related to the proteus/serratia pore-forming family of haemolysins. tn916 mutagenesis was employed to isolate hae ... | 1995 | 8825086 |
| specificity of the immune response to haemophilus ducreyi. | the specificity of the antibody response to haemophilus ducreyi in sera from patients attending a sexually transmitted disease clinic in south africa has been studied using immunoblotting. patients with chancroid were shown to have higher levels of igg (mean 0.74, sd 0.34) to h. ducreyi than those with no history of chancroid (mean 0.34, sd 0.19). the pattern of the antibody specificity was highly variable between patients with culture proven chancroid but there was no observed strain specificit ... | 1995 | 8559038 |
| comparison of azithromycin and ceftriaxone for the treatment of chancroid. | we conducted a randomized, unblinded, prospective study designed to determine the efficacy of single-dose azithromycin for the treatment of chancroid. men and women 16 years of age and older who had darkfield-negative genital ulcers that were clinically suspected to be caused by haemophilus ducreyi and who attended urban sexually transmitted disease clinics or presented to hospital emergency departments were enrolled in the study. patients were randomized to receive 250 mg of ceftriaxone im or 1 ... | 1995 | 8562752 |
| low dose erythromycin regimen for the treatment of chancroid. | haemophilus ducreyi is the commonest cause of genital ulcer disease in africa and is associated with heterosexual transmission of human immunodeficiency virus(hiv). the world health organization currently recommends erythromycin 500 mg three times a day for seven days as the treatment of choice for haemophilus ducreyi infection. we studied the effectiveness of a lower dose erythromycin treatment regime, 250 mg three times a day for seven days in the treatment of chancroid. patients with genital ... | 1995 | 8904044 |
| simple algorithms for the management of genital ulcers: evaluation in a primary health care centre in kigali, rwanda. | a cross-sectional study was conducted among 395 patients presenting with genital ulcers at a primary health care centre in kigali, rwanda. using clinical data and the results of a rapid plasma reagin (rpr) test, we simulated the diagnostic outcome of two simple who flowcharts for the management of genital ulcers. these outcomes and a clinical diagnosis were then compared with the laboratory diagnosis based on culture for genital herpes and haemophilus ducreyi and serology for syphilis. the preva ... | 1995 | 8907769 |
| sexually transmitted diseases and human immunodeficiency virus control in malawi: a field study of genital ulcer disease. | men with genital ulcer disease (gud) attending a clinic in malawi were evaluated and treated with one of five drug regimens. haemophilus ducreyi was isolated from 204 (26.2%) of 778 patients. of 677 men, 198 (29.2%) had treponemes detected in ulcer material by direct immunofluorescence or had rapid plasma reagin reactivity of > or = 1:8. human immunodeficiency virus type 1 (hiv-1) seroprevalence was 58.9% overall and 75.8% among patients reporting a history of gud (p < .001). by logistic regress ... | 1995 | 7844388 |
| incision and drainage versus aspiration of fluctuant buboes in the emergency department during an epidemic of chancroid. | to compare outcomes of incision and drainage with packing to needle aspiration of purulent buboes in patients with presumed chancroid. the safety and efficacy of incision and drainage were compared with that of needle aspiration. | 1995 | 7482103 |
| bacterial [cu,zn]-superoxide dismutase: phylogenetically distinct from the eukaryotic enzyme, and not so rare after all! | copper- and zinc-containing superoxide dismutases ([cu,zn]-sods) are generally considered almost exclusively eukaryotic enzymes, protecting the cytosol and extracellular compartments of higher organisms from damage by oxygen free-radicals. the recent description of a few examples of bacterial forms of the enzyme, located in the periplasm of different gram-negative micro-organisms, prompted a re-evaluation of this general perception. a pcr-based approach has been developed and used successfully t ... | 1995 | 7496539 |
| simplified pcr for detection of haemophilus ducreyi and diagnosis of chancroid. | a simplified pcr was developed for detection of haemophilus ducreyi in samples from chancroid patients. the strategy included a straightforward chloroform extraction sample preparation method, a one-tube nested pcr to minimize contamination risks, and a colorimetric method for detection of products. primers were designed from published nucleotide sequences of the 16s rrna gene of h. ducreyi, with longer outer primers for annealing at a higher temperature and shorter inner primers labelled with b ... | 1995 | 7540625 |
| chancroid: new developments in an old disease. | 1995 | 7546365 | |
| chancroid and haemophilus ducreyi: an update. | haemophilus ducreyi is a fastidious gram-negative bacillus that causes the sexually transmitted infection chancroid. chancroid is a major genital ulcerative disease in africa, southeast asia, the caribbean, and latin america and is of increasing concern in the united states. genital ulcerative disease and chancroid in particular have been associated with facilitating the transmission of human immunodeficiency virus. the diagnosis of chancroid based on the clinical appearance of the genital lesio ... | 1995 | 7553570 |
| detection of haemophilus ducreyi lipooligosaccharide by means of an immunolimulus assay. | a murine monoclonal antibody (mab) directed against a surface-exposed epitope of the lipooligosaccharide (los) of haemophilus ducreyi strain 35000 was shown to be reactive with all 37 strains of this pathogen tested in a colony blot-radioimmunoassay. the los epitope bound by this mab appeared to be stably expressed by h. ducreyi growing in vitro. the use of this mab in the immunolimulus system revealed that it could detect purified h. ducreyi los at a level of 25 pg/ml. similarly, this immunolim ... | 1995 | 7561133 |
| in vitro stimulation of peripheral blood mononuclear cells (pbmc) from hiv- and hiv+ chancroid patients by haemophilus ducreyi antigens. | the cellular immune responses to fractionated haemophilus ducreyi antigens, coated on latex beads, were assessed in patients with chancroid and in controls, using an in vitro lymphocyte proliferation assay. several fractions of h. ducreyi antigen revealed stimulating activity. however, only the molecular size ranges 91-78 kd, 59-29 kd, and 25-21 kd induced proliferation that may be specifically related to h. ducreyi infection. lymphocytes from four hiv- patients, successfully treated for chancro ... | 1995 | 7586673 |
| asymptomatic carriage of haemophilus ducreyi confirmed by the polymerase chain reaction. | to investigate asymptomatic carriage of haemophilus ducreyi by using polymerase chain reaction (pcr) on samples from women at high risk of infection. | 1995 | 7590712 |
| characterization of the hemolytic activity of haemophilus ducreyi. | h. ducreyi is the causative agent of chancroid, a genital ulcer disease most prevalent in developing countries. chancroid enhances the heterosexual transmission of human immunodeficiency virus and is identified in focal outbreaks in the united states, but little is known about its pathogenesis. we studied the hemolysin produced by h. ducreyi because this molecule might be an important virulence factor in the pathogenesis of chancroid. ten strains of h. ducreyi were tested on newly devised blood ... | 1995 | 7591078 |
| cytotoxin production in 100 strains of haemophilus ducreyi from different geographic locations. | one-hundred strains of haemophilus ducreyi, representing isolates from different parts of the world, including the reference strains, were obtained from different collections and characterized with special reference to cytotoxin production in vitro. the cytotoxic activity on cultured epithelial cells (hep-2) was examined with two methods. the activity in bacterial sonicates was tested on freshly trypsinated cells and strains manifesting little or no cytotoxic activity in sonicates were investiga ... | 1995 | 7607403 |
| chancroid detected by polymerase chain reaction--jackson, mississippi, 1994-1995. | chancroid is a sexually transmitted disease (std) caused by infection with haemophilus ducreyi and is characterized by genital ulceration. chancroid is underreported in the united states (1), reflecting, in part, difficulties in diagnosis because of clinical similarities between chancroid and other ulcerative stds. in addition, laboratory confirmation by culture is 53%-84% sensitive and often is unavailable in clinical settings (2). in september 1994, clinicians at the district v std clinic of t ... | 1995 | 7616954 |
| a system for generalized mutagenesis of haemophilus ducreyi. | the lack of a generalized mutagenesis system for haemophilus ducreyi has hampered efforts to identify virulence factors expressed by this sexually transmitted pathogen. to address this issue, the transposable element tn1545-delta 3, which encodes resistance to kanamycin, was evaluated for its ability to insert randomly into the h. ducreyi chromosome and produce stable, isogenic mutants. electroporation of h. ducreyi with 1 microgram of plasmid pms1 carrying tn1545-delta 3 resulted in the product ... | 1995 | 7622219 |
| swine model of haemophilus ducreyi infection. | haemophilus ducreyi is a strict human pathogen that causes sexually transmitted genital ulcer disease. we infected domestic swine with h. ducreyi 35000, resulting in the development of cutaneous ulcers histologically resembling human chancroid lesions. intraepidermal lesions progressed from pustules to ulcers containing polymorphonuclear leukocytes and were accompanied by a dermal inflammatory infiltrate containing t cells and macrophages. h. ducreyi was recovered from lesions up to 17 days afte ... | 1995 | 7622236 |
| chancroid epidemiology in new orleans men. | epidemiologic, clinical, and microbiologic data were collected from 299 men with nonsyphilitic genital ulcer disease. one hundred eighteen (39%) were culture-positive for haemophilus ducreyi, 57 (19%) were culture-positive for herpes simplex virus, and 124 (41%) were culture-negative. patients with chancroid were significantly more likely than those with genital herpes to have been frequent users of alcohol (44% vs. 23%, p = .006). they were also more likely recently to have used cocaine (25% vs ... | 1995 | 7622888 |
| localisation and immunological properties of a 24-kda surface protein of haemophilus ducreyi. | the cell wall and outer structures of haemophilus ducreyi bacteria were investigated. the 24-kda outer protein from two strains was purified with an sds-page preparative continuous-elution electrophoresis cell. the protein was further characterised by sds-page and immunoblotting, and the immunological properties were investigated by elisa. localisation on the bacterial surface was investigated by immuno-electron-microscopy with a polyclonal antiserum raised against the purified protein. a triple ... | 1995 | 7650727 |
| structural studies of lipooligosaccharides from haemophilus ducreyi itm 5535, itm 3147, and a fresh clinical isolate, acy1: evidence for intrastrain heterogeneity with the production of mutually exclusive sialylated or elongated glycoforms. | the structures of the lipooligosaccharides (loss) from haemophilus ducreyi itm 5535 and itm 3147 and a fresh clinical isolate, acy1, have been investigated. oligosaccharides were obtained from phenol-water-extracted los by mild acid hydrolysis and were studied by methylation analysis, fast atom bombardment and electrospray ionization mass spectrometry, and nuclear magnetic resonance spectroscopy. the major oligosaccharide obtained from all strains was a nonasaccharide with the structure beta-d-g ... | 1995 | 7665520 |
| use of tissue culture and animal models to identify virulence-associated traits of haemophilus ducreyi. | to identify virulence-associated properties of haemophilus ducreyi, 34 strains of this sexually transmitted pathogen were evaluated for in vitro phenotypic characteristics of potential relevance to chancroid pathogenesis and for their ability to produce lesions in the temperature-dependent animal model for chancroid. of the 34 strains tested, all but three produced a cytopathic effect on human foreskin fibroblasts (hff) and all but six strains formed large microcolonies on hff monolayers. a subs ... | 1995 | 7729882 |
| inducible immunity with a pilus preparation booster vaccination in an animal model of haemophilus ducreyi infection and disease. | using the temperature-dependent rabbit model of haemophilus ducreyi infection as a quantitative virulence assay, we tested the abilities of two bacterial antigen preparations to induce protection against subsequent infection and disease. lipooligosaccharide (los) and a pilus preparation were purified from h. ducreyi 35000 and were used in a booster immunization procedure. the serologic response to each immunogen was monitored by enzyme immunoassay. h. ducreyi virulence was assayed by intraepithe ... | 1995 | 7729916 |
| characterization of the hgba locus encoding a hemoglobin receptor from haemophilus ducreyi. | haemophilus ducreyi can bind hemoglobin and use it as a source of heme, for which it has an obligate requirement. we previously identified and purified hgba, a hemoglobin-binding outer membrane protein from h. ducreyi. in this report, we describe the molecular cloning, expression, dna sequence, and mutagenesis of the structural gene for hgba, hgba. h. ducreyi and recombinant escherichia coli expressing hgba bound [125i]hemoglobin, establishing hgba as a receptor. insertions or deletions in the c ... | 1995 | 7768598 |
| haemophilus ducreyi: pathogenesis and protective immunity. | haemophilus ducreyi is the etiological agent of chancroid, a sexually transmitted disease that is common in developing countries and that has characteristic genital mucocutaneous lesions. the adherence and growth of bacteria on the surface of eukaryotic cells, and the production of cytotoxin(s) result in cell damage that may be responsible for the development of ulcers. the mechanisms for protective immunity in chancroid are unclear, but both humoral and cell-mediated mechanisms may be involved. | 1995 | 7773593 |
| serum bactericidal activity and phagocytosis in host defence against haemophilus ducreyi. | serum bactericidal activity and phagocytic killing are two important mechanisms involved in the host defence against bacteria. using some in vitro methods, serum bactericidal assay, phagocytic killing by polymorphonuclear leukocytes (pmn) and chemiluminescence, we have evaluated the significance of these mechanisms in the killing of haemophilus ducreyi bacteria. furthermore, induction of c3 conversion and deposition of immunoglobulins, c1q and c3, on the surface of bacteria was studied by crosse ... | 1995 | 7783597 |
| monoclonal antibodies against haemophilus lipopolysaccharides: clone dp8 specific for haemophilus ducreyi and clone dh24 binding to lacto-n-neotetraose. | mouse monoclonal antibodies (mabs) dp8 [immunoglobulin g1(kappa)] and dh24 [immunoglobulin m(kappa)], which are specific for haemophilus ducreyi lipopolysaccharide (lps), were generated by fusing mouse myeloma ns0 cells with spleen cells of balb/c mice immunized with a total membrane preparation of h. ducreyi. mab dp8 reacted in whole-cell enzyme immunoassay (eia) and colony dot immunoblotting with all 50 strains of h. ducreyi but not with any other bacteria tested, which suggests an exposed and ... | 1995 | 7790083 |
| alterations in sample preparation increase sensitivity of pcr assay for diagnosis of chancroid. | a pcr assay for the detection of haemophilus ducreyi in clinical specimens taken from genital ulcers was developed. although h. ducreyi, when present in such specimens, could be detected by pcr, the sensitivity of the assay was reduced by the presence of taq polymerase inhibitors in the specimen. the sensitivity of the pcr assay was improved by the use of detergents in preparing nuclei acids from clinical specimens and by the inclusion of a dialysis step prior to amplification. in addition, sodi ... | 1995 | 7790433 |
| failure of treatment for chancroid in rwanda is not related to human immunodeficiency virus infection: in vitro resistance of haemophilus ducreyi to trimethoprim-sulfamethoxazole. | a comparative open study was performed to evaluate the efficacy of single doses of ciprofloxacin (500 mg) and trimethoprim-sulfamethoxazole (tmp-smz; 640 mg/3,200 mg) for the treatment of culture-proven chancroid. clinical cure or improvement was observed 7 days after treatment in 32 (76.2%) of the 42 patients who received ciprofloxacin and 21 (52.5%) of the 40 patients who received tmp-smz (p = .04). cultures for one (4.5%) of 22 patients not cured with ciprofloxacin and 16 (59.3%) of 27 patien ... | 1995 | 7795096 |
| recommendations for treatment of chancroid, 1993. | since the 1989 sexually transmitted diseases treatment guidelines were published by the centers for disease control and prevention, changes in the efficacy of the recommended and alternative regimens for the treatment of haemophilus ducreyi infections have been described. among recommended agents, erythromycin remains effective, and although a single dose of ceftriaxone appears to remain effective in the united states, limited data from kenya have shown that this regimen has been associated with ... | 1995 | 7795108 |
| identification and purification of a conserved heme-regulated hemoglobin-binding outer membrane protein from haemophilus ducreyi. | a hemoglobin-binding protein (hgba) from haemophilus ducreyi was identified and purified. the 100-kda hgba was detected in all strains of h. ducreyi tested, and a somewhat larger hemoglobin-binding protein was found in one strain of haemophilus influenzae. hgba was purified and the amino acid sequence of the n terminus of hgba revealed no significant homologies with known proteins. two different antisera to hgba from h. ducreyi 35000 recognized hgba proteins from all tested h. ducreyi strains; t ... | 1995 | 7890379 |
| structure of the major oligosaccharide from the lipooligosaccharide of haemophilus ducreyi strain 35000 and evidence for additional glycoforms. | haemophilus ducreyi is a sexually transmitted pathogen that colonizes the genital epithelium in humans, causing genital ulcers or chancroid. its surface lipooligosaccharides (loss) have been shown to play a role in ulcer formation and may also be important in cell adhesion and invasion of host tissue. earlier we presented a preliminary structure of the major los from strain 35000 that suggested the presence of terminal lactosamine [melaugh, w., phillips, n.j., campagnari, a.a., karalus, r., & gi ... | 1994 | 7947712 |
| haemophilus ducreyi attaches to and invades human epithelial cells in vitro. | haemophilus ducreyi is a sexually transmitted pathogen that causes genital ulcers and inguinal adenopathy. because chancroidal ulcers are most commonly located on the foreskins of uncircumcised males, we utilized human foreskin epithelial cells (hfecs) to investigate the initial interaction of h. ducreyi with its host. the eight different strains of h. ducreyi that were studied varied in their abilities to attach to these epithelial cells, with six strains consistently attaching to > or = 90% of ... | 1994 | 7960145 |
| single dose azithromycin for the treatment of chancroid: a randomized comparison with erythromycin. | chancroid is endemic in sub-saharan africa and enhances the sexual transmission of the human immunodeficiency virus type 1 (hiv-1). azithromycin is an orally absorbed macrolide antibiotic that is active against haemophilus ducreyi, the causative agent of chancroid, and has pharmacokinetic properties that are suitable for single dosing. | 1994 | 7974076 |
| [chancroid in yaounde. apropos of 42 cases]. | in yaoundé, chancroid occurs frequently among adolescents and young men (95 p. 100 of the cases) who have sexual contacts regularly with prostitutes. apart of classical clinical forms complicated in 2/3 of cases by inguinal adenopathies, we have observed furuncular chancroid which is quite characteristic of the disease. giemsa stain represents for us a simple and reliable diagnostic method for this disease because in 1/3 of smears, typical cultural aspects of haemophilus ducreyi were seen. | 1994 | 7979020 |
| emergence of haemophilus ducreyi resistance to trimethoprim-sulfamethoxazole in rwanda. | the in vitro susceptibilities of 112 clinical isolates of haemophilus ducreyi to six antimicrobial agents were determined. these isolates were obtained in kigali, rwanda, during three studies on genital ulcer disease performed in 1986 (18 isolates), 1988 (23 isolates), and 1991 (71 isolates). all h. ducreyi isolates were susceptible to azithromycin, ceftriaxone, ciprofloxacin, and erythromycin; all isolates obtained in 1986 were also susceptible to trimethoprim and to the combination trimethopri ... | 1994 | 7979300 |
| clear broth and plate media for culture of haemophilus ducreyi. | using catalase as a source of heme, we have developed both clear plate and broth media for the growth of haemophilus ducreyi, the causative agent of chancroid. in the broth medium, the growth phase of the organism can be monitored and the organisms achieve a cell density of > 10(8) cfu/ml. | 1994 | 7989562 |
| [importance of culture media choice in the isolation of haemophilus ducreyi. experience in senegal]. | genital ulcerations typify one of the major reasons clients seek std consultation in developing countries. the usual etiologies are syphilis, chancroid and herpes. the ideal diagnostic approach is to undertake complete laboratory examination that are rarely possible in structure destitute of laboratory analysis possibilities which is the case for most of the std transmission agents. chancroid is caused by haemophilus ducreyi, a short gram negative bacteria. the bacteriological diagnosis is based ... | 1994 | 8003900 |
| identification of a hemolytic activity elaborated by haemophilus ducreyi. | haemophilus ducreyi is the causative agent of the sexually transmitted disease chancroid. we have identified a hemolytic activity expressed by h. ducreyi. this activity is most readily detected when horse erythrocytes are used as a target; however, low levels of activity can be detected with sheep, human, or rabbit erythrocyte targets. the activity is heat labile and protease sensitive. | 1994 | 8005696 |
| recent changes in the epidemiology of genital ulcer disease in the united states. the crack cocaine connection. | the incidence of syphilis and chancroid began to increase in the united states among heterosexuals in the mid-1980s, with most cases reported among minorities living in eastern cities and in the south. a number of studies have established a link between increasing syphilis incidence rates and cocaine use, specifically the smoked form of the drug, which is known as "crack." a similar link was hypothesized for chancroid, but supporting data became available only recently. in new orleans, we showed ... | 1994 | 8042123 |
| [urethro-adnexitis in the man and acute urethral syndrome in the woman. microbiological and immunologic studies of etiologic classification]. | both common pathogens and unconventional, fastidious bacteria, viruses, parasites, and fungi are causative agents in male urethro-adnexitis and in female acute urethral syndrome. uropathogens, neisseria gonorrhoeae, treponema pallidum, mycobacterium tuberculosis, chlamydia trachomatis, mycoplasma spp., haemophilus ducreyi, calymmatobacterium granulomatis, gardnerella vaginalis, anaerobic bacteria, herpes simplex virus type ii (hsv ii), papillomaviruses (hpv), trichomonas vaginalis and candida sp ... | 1994 | 8053087 |
| haemophilus ducreyi adheres to human keratinocytes. | haemophilus ducreyi, moraxella catarrhalis and a non-piliated escherichia coli k-12 strain were studied for their ability to bind to human keratinocytes in vitro. epidermal cells isolated from neonatal foreskins were grown to confluence in serum-free keratinocyte media. probing of the monolayers with anti-cytokeratin antibody showed that 97% of cells were keratinocytes. bacteria were grown to mid-log phase and seeded onto the monolayers. at various time-points monolayers were washed with pbs to ... | 1994 | 8090082 |
| characterization of the cytopathic effect of haemophilus ducreyi. | haemophilus ducreyi is the etiologic agent of chancroid, which is a genital ulcer disease that increases the risk of acquiring and transmitting hiv. the pathogenesis of h. ducreyi is not well understood. | 1994 | 7817258 |
| seroepidemiological studies of haemophilus ducreyi infection in ethiopian women. | to measure prevalence of anti-haemophilus ducreyi antibodies in sera from ethiopian female attendees, and to determine significant socioeconomic associations. | 1994 | 7817262 |
| seroprevalence and incidence of sexually transmitted diseases in a rural ugandan population. | the aim of the study was to determine in a rural population the age- and sex-specific prevalence and incidence rates of serological reactivity of 5 common sexually transmitted diseases (stds) and their association with hiv-1 antibody status. of the adult population of two villages (529 adults aged 15 years or more) 294 provided an adequate blood specimen both on enrollment and at 12 months. the sera were tested at 3 collaborating laboratories for antibodies against hiv-1, treponema pallidum, hae ... | 1994 | 7819350 |
| teenage obstetric and gynaecological problems in an african city. | to measure the prevalence of sexually transmitted diseases (std), pelvic inflammatory disease (pid), cervical cancer, pregnancy and use of contraception in teenagers, and to determine socioeconomic factors associated with these conditions to aid planners of medical services and promotion of sexual health. | 1994 | 7834712 |
| induction of protective immunity to haemophilus ducreyi in the temperature-dependent rabbit model of experimental chancroid. | the temperature-dependent rabbit model for chancroid, a sexually transmitted disease caused by the fastidious gram-negative bacterium haemophilus ducreyi, was used to investigate the abilities of previous infection and immunization with an acellular preparation of h. ducreyi to induce protective immunity. in the first set of experiments, animals were infected intradermally with either the 35000 or cha-1 strains of h. ducreyi and then rechallenged 30 days later with both the homologous and hetero ... | 1994 | 8254191 |
| a quantitative chemiluminescent ribosomal probe method for monitoring adherence of haemophilus ducreyi to eukaryotic cells. | this study evaluated a commercially available chemiluminescent-labelled, ribosomal rna-directed dna probe (crp) as a method to quantitate attachment of h. ducreyi to human foreskin fibroblast (hff) cells. evaluation of four strains of h. ducreyi demonstrated that the crp assay was unaffected by eukaryotic cells and its advantages were: (i) quantitation was done after attachment so it did not interfere with the attachment process, and (ii) it was a rapid, reliable method for quantitating bound ba ... | 1994 | 7700133 |
| an analysis of the complete nucleotide sequence of the haemophilus ducreyi broad-host-range plasmid pls88. | we present an analysis of the complete nucleotide sequence of pls88, a naturally occurring, 4.8-kb broad-host-range plasmid isolated from haemophilus ducreyi and encoding resistance to sulfonamides, streptomycin, and kanamycin. sequence analysis of the genes encoding sulfonamide and streptomycin resistance revealed homology to the rsf1010 sulii and stra genes. the sulii-stra intergenic region of pls88 has a 38-bp deletion identical to that of the rsf1010-like plasmid phd8.1, isolated from actino ... | 1994 | 7846148 |
| human t cell lymphotropic virus type i infection among female sex workers in peru. | four hundred female sex workers attending a sexually transmitted disease clinic in lima, peru, were interviewed for demographic information and medical, contraceptive, and sexual practice histories. cervical cultures were done for neisseria gonorrhoeae and chlamydia trachomatis, and serum was tested for antibodies to human immunodeficiency virus, human t cell lymphotropic virus type i (htlv-i), treponema pallidum, c. trachomatis, herpes simplex virus type 2 (hsv-2), and haemophilus ducreyi. the ... | 1994 | 8133088 |