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beta-lactamase stability of faropenem.faropenem (far) is an orally available member of the penem class unique among carbapenems and other available beta-lactams. this study compared far to cephalosporins and imipenem with respect to beta-lactamase (bla) stability and emergence of resistance to staphylococcus aureus and escherichia coli. bla stability was studied using enzyme preparations from sonicated/centrifuged 24-hour cultures of e. coli, enterobacter cloacae, proteus vulgaris, providencia rettgeri, klebsiella pneumoniae, s. aur ...200314504433
resection of a massive arteriovenous malformation of the head and neck.we report a patient with a massive arteriovenous malformation of the head and neck treated by surgical extirpation of the lesion. arteriography, hypotensive hypothermic anesthesia, and the intraoperative use of the doppler flowmeter were most helpful in this case.2003154679
contact dermatitis from polyfunctional acrylic monomers.five out of 26 men employed formulating radiation drying printing ink developed eczematous dermatitis. positive patch tests were obtained with two polyfunctional acrylic monomers, pentaerythritol triacrylate and trimethylol propane triacrylate and with formulations containing these in four affected employees. cross sensitization was observed to dipentaerythritol monohydroxy pentaacrylate in each case. a fifth employee appeared to have developed irritant dermatitis from contact with these materia ...2003145343
[relation between endocrinology and psychiatry. some recent developments].relations between endocrinology and psychiatry are developing in two main directions: behavioural effects of hormones. after the effects of peripheral hormones, recent works are concerned with a.c.t.h. and antidiuretic hormone (which interfere with memory process) and with hypothalamic peptides (which might have psychotropic properties, independant of their endocrine regulation function). -correlations between psychiatric syndroms and endocrine disorders. in some mentally depressed patients, dis ...2003165466
[gastroschisis and omphalocele. treatment and results]. 2003150087
improving the specific synthetic activity of a penicillin g acylase using dna family shuffling.penicillin g acylas (pga) of providencia rettgeri (atcc 25599) was evolved using a modified dna family shuffling method. the identity of pga genes from escherichia coli, kluyvera citrophila and providencia rettgeri ranges from 62.5% to 96.9%. the pga genes from above three species were recombined and shuffled to create interspecies pga gene fusion libraries. by substituting assembled chimaeras for corresponding region of petppga, different recombinants were constructed and expressed in e. coli j ...200312796820
prevalence of extended-spectrum beta-lactamases produced by nosocomial isolates of enterobacteriaceae in trakya university hospital, turkey.the prevalence of extended-spectrum beta-lactamase (esbl) production by 194 nosocomial isolates of enterobacteriacea recovered from 1995 to 1999 was investigated. the esbl production was determined by the double-disk synergy test and was confirmed by the e-test esbl strip. twenty-three isolates (21 klebsiella pneumoniae, one escherichia coli, one providencia rettgeri) were found as esbl-producers (11.8%). these isolates were also usually resistant to non-betalactam antibiotics. most of them cont ...200312901421
[management of aplastic anemia in pregnancy and delivery]. 2003309014
incidence of dental caries in lucknow school-going children. 2003296202
separation of tetracyclines by high-speed liquid chromatography.the separation of tetracycline and its four commoninpurities has been studied by high-speed liquid chromatography. a preliminary study of the effectiveness of ion-exchange, adsorption, liquid-liquid partition and reversed-phase ion-pair chromatography indicated that only the last method showed promise. by developing special hydrocarbon-bonded stationary phases a rapid and complete resolution of all five tetracyclines has been obtained within 10 min. plate heights using a derivatised 18-mum parti ...2003166085
corneal manifestations of the systemic mucopolysaccharidoses.newer biochemical understanding of the mucopolysaccharidoses now allows a better classification of these diseases. the dermatan and keratan sulfate-storing diseases have corneal clouding. the heparan sulfate-storing diseases have retinal changes and usually central nervous system manifestations.2003156516
the relationship of the pulp and periodontium. 2003149297
the investigation of biopolymers in dilute solutions with x-ray small-angle scattering. 2003201141
pseudogout: clinical observations and chemical analyses of deposits. 2003181012
how does the depressed patient fare in a family practice? 2003194945
[primary disability of patients with bone fractures in saratov]. 2002126406
laparoscopy in hepatic disease. 2002134923
design of living environments for nursing-home residents: increasing participation in recreation activities.nursing-home residents have frequently been characterized as unoccupied and disengaged. at the outset of the present study, most residents were to be found in their own rooms, not exhibiting gross motor behavior or social interaction, and not participating in appropriate activities. to modify residents' levels of participation with the environment, a manipulative area was provided in the lounge. participation in the lounge averaged 20% on days when the activity was not available, but increased t ...2002126983
high-level secretory expression of penicillin amidase from providencia rettgeri in saccharomyces cerevisiae: purification and characterization.heterologous production of the heterodimeric penicillin g amidase (pac) from providencia rettgeri was optimized in saccharomyces cerevisiae. several factors, including the effect of different growth and induction conditions, were identified to be critical for the enzyme overproduction and secretion. the pac yield was significantly increased by more than 500-fold compared to that obtained in the native bacterium, and the recombinant enzyme was almost entirely secreted. electrophoretic characteriz ...200211934304
effect of ph on high-temperature production of bacterial penicillin acylase in escherichia coli.high-temperature-oriented production of bacterial penicillin acylase (pac), which is usually expressed at low temperatures (less than 30 degrees c), was demonstrated in this study via heterologous expression of the providencia rettgeri (p. rettgeri) pac gene in escherichia coli (e. coli). while it is possible to produce pac at a temperature as high as 37 degrees c, the environmental condition (specifically, culture ph) critically affected culture performance. production of pac at 37 degrees c wa ...200212052092
bacterial population of a two-phase anaerobic digestion process treating effluent of cassava starch factory.different groups of microorganisms in a two-phase anaerobic system were enumerated to evaluate the prevalence of specific groups and species. total and fecal coliforms showed similar values both in acidogenic and methanogenic reactors. the fecal streptococci were 4-fold higher in the acidogenic reactor, when compared with those of the methanogenic reactor. as expected, no methane forming or sulfate reducing bacteria were found in the acidogenic reactor. the populations of methanogenic bacteria w ...200212088382
r391: a conjugative integrating mosaic comprised of phage, plasmid, and transposon elements.the conjugative, chromosomally integrating element r391 is the archetype of the incj class of mobile genetic elements. originally found in a south african providencia rettgeri strain, r391 carries antibiotic and mercury resistance traits, as well as genes involved in mutagenic dna repair. while initially described as a plasmid, r391 has subsequently been shown to be integrated into the bacterial chromosome, employing a phage-like integration mechanism closely related to that of the sxt element f ...200212193633
osteomyelitis associated with salmonella enterica ss arizonae in a colony of ridgenose rattlesnakes (crotalus willardi).the identification of three arizona ridgenose rattlesnakes (crotalus willardi) with salmonella arizonae-associated osteomyelitis led to a 5-yr prospective study of radiographic signs and salmonella intestinal carriage rates in a 19-member colony of this rattlesnake species. ventrodorsal radiographs were performed and cloacal swabs were cultured for salmonella spp. annually. ten snakes survived the 5-yr period, with six of them remaining free of bony lesions. three snakes that had no bony lesions ...200212564525
comparison of sxt and r391, two conjugative integrating elements: definition of a genetic backbone for the mobilization of resistance determinants.the sxt element (sxt) is becoming an increasingly prevalent vector for the dissemination of antibiotic resistances in vibrio cholerae. sxt is a member of a larger family of elements, formerly defined as incj plasmids, that are self-transmissible by conjugation and integrate site-specifically into the host chromosome. comparison of the dna sequences of sxt and r391, an incj element from providencia rettgeri, indicate that these elements consist of a conserved backbone that mediates the regulation ...200212568332
production of glycosylated thermostable providencia rettgeri penicillin g amidase in pichia pastoris.penicillin g amidase from providencia rettgeri is a heterodimer of 92 kda. we have previously expressed the pr. rettgeri pac gene coding for this enzyme in saccharomyces cerevisiae, and now we report the expression and characterization in the methylotrophic yeast pichia pastoris. the recombinant catalytically active enzyme (rpac(pr)) was secreted from shake flask-grown p. pastoris cells into the medium at a level of approximately 0.18 u ml(-1). this yield of rpac(pr) was higher, by two orders of ...200212702330
[disabled children in the federal republic of germany in 1974 (author's transl)].within the scope of an additional micro-census inquiry--carried out in 1974--the federal statistical office in wiesbaden assessed the number of physically, mentally and psychologically impaired children. the results are differentiated according to the types of disability and therapy, age, in-patient treatment and special school attendance. furthermore, the estimated results are compared with earlier surveys of the federal statistical office and other estimates.2002135318
chemical hazards in the workplace. 2002135215
complications associated with therapeutic soft contact lenses.the complications of therapeutic hydrophilic contact lens wear are systematically outlined. hypoxia is the major cause of initial patient failure as well as the long-term problems of corneal edema, neovascularization, and susceptibility to infection. the common difficulties with lens breakage, lens deposits, poor lens retention, and other entities are discussed. suggestions for avoiding and treating various complications are given, including updated indications and contraindications for fitting ...200193261
hyperammonemic coma caused by providencia rettgeri infection in a child with prune belly syndrome.objective: to report a case of hyperammonemic encephalopathy in the absence of liver disease caused by a urinary tract infection with a previously unreported organism. design: case report. setting: pediatric intensive care unit. patient: critically ill child who presented with hyperammonemic coma. intervention: bladder drainage and antimicrobial therapy for the urinary tract infection. measurements: rapid resolution of the hyperammonemia. main results: rapid improvement in the patient's neurolog ...200112797879
formation of chromosomal tandem arrays of the sxt element and r391, two conjugative chromosomally integrating elements that share an attachment site.the sxt element, a conjugative, self-transmissible, integrating element (a constin) originally derived from a vibrio cholerae o139 isolate from india, and incj element r391, originally derived from a south african providencia rettgeri isolate, were found to be genetically and functionally related. both of these constins integrate site specifically into the escherichia coli chromosome at an identical attachment site within the 5' end of prfc. they encode nearly identical integrases, which are req ...200111157923
isolation and identification of bacteria associated with adult laboratory mexican fruit flies, anastrepha ludens (diptera: tephritidae).from the guts of new and old colonies (female and male) of mexican fruit flies, anastrepha ludens (diptera: tephritidae), we identified a total of 18 different bacterial species belonging to the family enterobacteriaceae, pseudomonadaceae, vibrionaceae, micrococcaceae, deinococcacea, bacillaceae, and the genus listeria. enterobacter, providencia, serratia, and staphylococcus spp. were the most frequently isolated genera, with citrobacter, streptococcus, aerococcus, and listeria found less freque ...200111178731
a case of purple urine bag syndrome associated with providencia rettgeri. 200111328846
antibiotic selective pressure and development of bacterial resistance.this study evaluates the development of resistance in gram-negative rods to cefotaxime and ceftazidime, ofloxacin and ciprofloxacin, gentamicin and amikacin, meropenem and ampicillin/sulbactam over a five year period of use (1994-1998) at the university hospital in olomouc, czech republic. the development of bacterial resistance was linked with antibiotic use and hence selective pressure which was specific for the type of antibiotic and the bacterial species. statistically significant correlatio ...200111337221
fluorogenic assay for penicillin g acylase activity.a simple, highly sensitive, and rapid assay for high-throughput screening of penicillin g acylase-producing bacteria is presented. the method is based on the specific release of fluorescent 7-amino-4-methyl-coumarin through cleavage of phenylacetyl-4-methyl-coumaryl-7-amide by penicillin g acylase. the present method is suitable for screening pure enzymes as well as various penicillin g acylases like those from escherichia coli, proteus rettgeri, and kluyvera citrophila in cell extracts. in addi ...200111355855
bacterial flora of the conjunctiva and nasal cavity in normal and diseased captive bustards.a survey was carried out to describe the normal aerobic bacterial flora of the conjunctiva and nasal cavity of captive houbara bustards (chlamydotis undulata), kori bustards (ardeotis kori), and white-bellied bustards (eupodotis senegalensis) maintained at the national avian research center, abu dhabi, united arab emirates. a total of 58 samples were examined from the nasal cavity and 55 samples from the conjunctiva of healthy bustards. there was no bacterial growth in 45% of conjunctival sample ...200111417826
activity of levofloxacin and ciprofloxacin against urinary pathogens.this study compares the antibacterial activities of levofloxacin and ciprofloxacin against recently isolated urinary tract pathogens, by evaluating their mics and mbcs in accordance with nccls susceptibility tests, time-kill curves and interference with bacterial adhesion to uroepithelial cells. a total of 200 clinical isolates was tested, including the species escherichia coli, klebsiella pneumoniae, proteus mirabilis, proteus vulgaris, providencia rettgeri, pseudomonas aeruginosa, enterococcus ...200111418511
[bacteremia due to providencia rettgeri]. 200111440672
chemical composition and cytotoxic and antimicrobial activity of calycotome villosa (poiret) link leaves.the chemical composition of the essential oil and methanol extract of calycotome villosa (poiret) link leaves collected in sardinia (italy) has been studied by analytical and spectroscopic methods. falcarinol and some alcohols, terpenes, furan derivatives, and paraffins have been isolated from the essential oil. thirteen alkaloids and falcarinol have been identified in the chloroform fraction of the basic methanol extract. six flavonoids and four anthraquinones have been isolated in the chlorofo ...200111482772
lymphocyte capping and carrier detection in duchenne muscular dystrophy. 200190791
research needs in low vision.research needs are presented for several areas of low vision: (1) reading and learning, (2) new low-vision aids, (3) modifying the environment, (4) utilization of disciplines, (5) new technologies, and (6) integrating low-vision services.200188182
in vitro and in vivo induction of effector t cells mediating dth responses to a protein and a synthetic polypeptide antigen. 200190558
prostatic acid phosphatase and prostatic cancer. 200188065
[complex intraoperative diagnosis of intracerebral tumors of the cerebral hemispheres]. 200190438
[questions to television from psychopathology].progress is expected in psychopathological diagnosis by using audiovisual procedures. nowadays, a valid psychopathology concept should be broadly planned and the important method of description as well as understanding procedures should be considered. for such a model of psychopathology it seems appropriate to investigate the different psychopathological phenomenons as expression of a disturbed communication ability. concerning this outlook, new strategies are to be developed or procedures of ot ...200190381
needle biopsy of the pancreas. 200190278
oestrogen therapy and endometrial carcinoma. 200187699
the collective and the personal. 200190674
tardive dyskinesia. 200190894
new trends in the preparation and storage of platelets. 20011731433
the search for indices. 200091795
[madame g.'s hyperthyroidism]. 200089684
an approach for enhancing heterologous production of providencia rettgeri penicillin acylase in escherichia coli.heterologous production of providencia rettgeri penicillin acylase (pac) was optimized in escherichia coli. several factors, including carbon, temperature, and host effects, were identified to be critical for the enzyme overproduction. the optimum culture conditions for the enzyme production vary for different host/vector systems. with the optimization, both volumetric and specific pac activities could be significantly improved by more than 50-fold compared to the native expression in p. rettger ...200010835229
in vitro activity of rifaximin, metronidazole and vancomycin against clostridium difficile and the rate of selection of spontaneously resistant mutants against representative anaerobic and aerobic bacteria, including ammonia-producing species.rifaximin is a rifamycin derivative characterized by a wide antibacterial activity. this drug is neither absorbed by the gastrointestinal tract nor inactivated by gastric juices, and exerts its action entirely within the intestinal lumen.200010859431
gut bacteria may be involved in interactions between plants, herbivores and their predators: microbial biosynthesis of n-acylglutamine surfactants as elicitors of plant volatiles.n-acylamino acids are dominant and widespread constituents of insect oral secretions (regurgitants), serving the insect as biosurfactants in the digestive process. during feeding the conjugates may be introduced into damaged leaves and contribute there to the elicitation of plant defenses such as the induction of volatile biosynthesis. from gut segments of spodoptera exigua, mamestra brassicae and agrotis segetum 23 bacterial strains were isolated, ten of which were able to synthesise typical le ...200011030433
natural antibiotic resistance of bacteria isolated from larvae of the oil fly, helaeomyia petrolei.helaeomyia petrolei (oil fly) larvae inhabit the asphalt seeps of rancho la brea in los angeles, calif. the culturable microbial gut contents of larvae collected from the viscous oil were recently examined, and the majority (9 of 14) of the strains were identified as providencia spp. subsequently, 12 of the bacterial strains isolated were tested for their resistance or sensitivity to 23 commonly used antibiotics. all nine strains classified as providencia rettgeri exhibited dramatic resistance t ...200011055901
diversity and contribution of the intestinal bacterial community to the development of musca domestica (diptera: muscidae) larvae.the bacterial diversity in the intestinal tract of musca domestica l. was examined in larvae collected from turkey bedding and corn silage. aerobic culturing yielded 25 bacterial species, including 11 from larvae collected from turkey bedding and 14 from larvae collected from corn silage. providencia rettgeri (hadley, elkins & caldwell) was the only species common to both environments. two mammalian pathogens, yersinia pseudotuberculosis (pfeiffer) and ochrobactrum anthropi (holmes), were isolat ...200011126551
progress with several models for the study of the sar of hallucinogenic agents. 2000101879
in vitro analysis of allogeneic lymphocyte interaction. iv. dual recognition of b cell-associated mls locus and i-region determinants by a helper allogeneic effect factor (aef) generated across a minor h locus disparity.a helper allogeneic effect factor (aef) was produced across an incompatibility at the minor histocompatibility loci. this aef is genetically restricted in its activity since it helps b cells only of the stimulator haplotype and of haplotypes that share both an mls and i-region identity with the stimulator haplotype. the i-region genes involved here map to the i-a and/or i-c subregions. an anti-lym immunoadsorbent column but neither an anti-h-2 nor an anti-ia column absorbed aef helper activity. ...200087470
pattern and flash visual evoked responses in multiple sclerosis.in multiple sclerosis (ms), increase of delay may be important, but the earlier change is the behavior of the wave shape; amplitude is always lower even in ms patients without visual subjective symptomatology, provided not only flash but also pattern stimulation is performed. normal amplitude with pattern stimulation does not necessarily mean normal with flash. combining both stimulation methods, we obtained lowering of amplitude and change of wave shape in 100% of cases while latency was increa ...200087495
fluorochrome staining of multilamellar liposomes.multilamellar liposomes can be stained with such fluorochromes as acridine orange, eosin y, neutral red, and thiazine red. the liposomes are brought into a 1% solution of the fluorochrome; 5-10 minutes later they are centrifuged and washed by resuspending in water or phosphate buffered saline three times. the last pellet is resuspended and a drop studied with the fluorescence microscope (1000 x magnification). the fluorochrome is seen to be accumulated in the liposomal membranes. acridine orange ...200089716
case report: treatment of posterior bite collapse with secondary occlusal traumatism. 200089659
[quantitative determination of copper in urine by color reaction with lead diethyldithiocarbamate]. 200090204
[nursing report. programs for clinical training of adult nursing (surgical nursing)]. 200087545
captopril in a hyponatraemic hypertensive: need for caution in initiating therapy. 199985145
the association of hla--b8 with atrophic thyroiditis.one-hundred-and-forty-seven patients with autoimmune thyroiditis were studied with respect to hla antigens as they related to various clinical features. hla--b8 was found to be significantly increased among 59 patients with atrophic thyroiditis (57% vs. 26% for controls) but was identical to controls in 88 patients with goitrous thyroiditis (26%). no relation was found in either group between b8 and thyroid autoantibody titer or, in the case of goitrous thyroiditis, the rate of progression of th ...199991210
epidermal cytoplasmic antigens. 199985677
antacids and cricoid pressure in the prevention of fatal aspiration syndrome. 199989406
non-specific disturbance of balance. 199989341
successive generations of child maltreatment. the children.families were identified, in north-east wiltshire (uk), (population 200,000), in which there was a pattern of two or more generations of child maltreatment and where there were also two or more children maltreated in the current generation (born between 1960 and 1980). these children had high rates of mental handicap, backwardness, and antisocial behaviour, and there were also characteristic patterns of emotional disturbance. the post-neonatal death rate for the 294 index children was very high, ...19993250697
thyrotoxicosis during pregnancy.the diagnosis of thyrotoxicosis during pregnancy is difficult on clinical grounds only. the determination of free thyroid hormones or free thyroid hormone indices is important and is possibly supplemented by the trh stimulation test. thyrotoxicosis during pregnancy should always be treated actively. the authors recommend subtotal thyroidectomy if there are indications for surgical therapy (nodular goitre or large diffuse goitre). otherwise treatment with thyrostatic agents is used. after operati ...199984547
biogenic amines derived from tryptophan in systemic and cutaneous scleroderma.blood levels of serotonin (5-ht) and urinary excretion of 5-hydroxyindole-acetic acid (5-hiaa), tryptamine (t), and indole-acetic acid (iaa) were determined in 39 cases of systemic scleroderma and in 7 cases of very severe cutaneous scleroderma. the t/iaa ratio was normal and serotonin elevated in mild and rather severe acrosclerosis alike, i.e. in systemic scleroderma with pronounced vascular involvement. the t/iaa ratio was increased and serotonin normal in severe acroscleroderma, representing ...199984460
cryoanalgesia and day-case herniorraphy. 199989546
synthesis and secretion of providencia rettgeri and escherichia coli heterodimeric penicillin amidases in saccharomyces cerevisiae.the providencia rettgeri and escherichia coli pac genes encoding heterodimeric penicillin g amidases (pac) were successfully expressed in saccharomyces cerevisiae. furthermore, these recombinant enzymes are secreted from the yeast cell into the medium which is in contrast to bacterial hosts, where the enzymes are retained in the periplasm. contrary to the p. rettgeri pac-encoding gene, the e. coli pac is poorly expressed in yeast. the highest yield of p. rettgeri pac was obtained with a multi-co ...199910072775
microbiology of the oil fly, helaeomyia petrolei.helaeomyia petrolei larvae isolated from the asphalt seeps of rancho la brea in los angeles, calif., were examined for microbial gut contents. standard counts on luria-bertani, macconkey, and blood agar plates indicated ca. 2 x 10(5) heterotrophic bacteria per larva. the culturable bacteria represented 15 to 20% of the total population as determined by acridine orange staining. the gut itself contained large amounts of the oil, had no observable ceca, and maintained a slightly acidic ph of 6.3 t ...199910103240
antibacterial activity of gentamicin and ciprofloxacin against gram-negative bacteria: interactions with pig and calf sera.the antibacterial activity of pig and calf serum and its ability to interact with gentamicin and ciprofloxacin were studied in vitro using escherichia coli k-12, proteus rettgeri (sanelli) and klebsiella pneumoniae atcc 10031. the antimicrobial activity of the above drugs, alone or in combination with serum, was investigated by the checkerboard method and expressed as the minimal inhibitory concentration (microg ml-1). pig serum (25%) with gentamicin had a synergistic antibacterial effect agains ...199910208763
prevalence of proteus species in urinary tract infections in a regional hospital in trinidad.proteus bacteria are a well-known cause of urinary tract infections (utis). the prevalence of utis is high among catheterized patients and those undergoing manipulation of the urinary tract. this study assessed the prevalence of utis due to proteus species, the predisposing factors, complications and extent of antimicrobial resistance at a regional teaching hospital.199910418178
tem-24 produced by four different species of enterobacteriaceae, including providencia rettgeri, in a single patient.four species of members of the family enterobacteriaceae harboring extended-spectrum beta-lactamase (esbl) were recovered in a single patient hospitalized in an intensive care unit. among these isolates, we describe for the first time an esbl-producing providencia rettgeri strain. bacteria from the same species were shown to be genetically related by pulsed-field gel electrophoresis analysis. these strains produced the same tem derivative esbl, characterized as tem-24. this enzyme had the peculi ...199910428940
molecular characterization of the genera proteus, morganella, and providencia by ribotyping.the so-called proteus-providencia group is constituted at present by three genera and 10 species. several of the recognized species are common opportunistic pathogens for humans and animals. different methods based on the study of phenotypic characters have been used in the past with variable levels of efficiency for typing some species for epidemiological purposes. we have determined the rrna gene restriction patterns (ribotypes) for the type strains of the 10 different species of the genera pr ...199910449462
development of bacterial resistance to the third generation cephalosporins and their clinical use.development of gram-negative rods resistance to the third generation cephalosporins (cefotaxime, ceftazidime, cefoperazone) in connection with their application at the university hospital in olomouc was evaluated in this study. the highest increase in resistance to cefotaxime was detected in enterobacter cloacae (from 22.9% in 1995 to 49.0% in 1997) and enterobacter agglomerans strains (28.0% - 40.5%). in addition, increased resistance to ceftazidime in acinetobacter baumannii (12.5% - 35.1%), e ...199910465127
crystal structure of penicillin g acylase from the bro1 mutant strain of providencia rettgeri.penicillin g acylase is an important enzyme in the commercial production of semisynthetic penicillins used to combat bacterial infections. mutant strains of providencia rettgeri were generated from wild-type cultures subjected to nutritional selective pressure. one such mutant, bro1, was able to use 6-bromohexanamide as its sole nitrogen source. penicillin acylase from the bro1 strain exhibited an altered substrate specificity consistent with the ability of the mutant to process 6-bromohexanamid ...199910548042
two cases of fatal transfusion-associated bacterial sepsis provoked by providencia rettgeri. 199910553171
[current status and perspectives of bioethics in argentina].the study of bioethics in argentina owes much to the efforts of josé alberto mainetti, who in 1972 founded the institute of medical humanities, devoted to researching, teaching, and publicizing the discipline. because of growing interest in the field of bioethics, a bioethics center was created in the institute in 1987 and it has stimulated the organization of several groups in various provinces in the country. one of the most important of them, located at the national university of mar del plat ...19992144132
hospitalization of the elderly patient for acute illness.there is a paucity of studies on the characteristics of elderly patients who are admitted for treatment of acute disorders, even though their utilization of hospitals is high. this study involved 828 older persons admitted to a general hospital during a period of six months, for the treatment of acute illness. the typical patient appeared to be a woman in her 70's who entered the medical department because of a cardiovascular or a gastrointestinal disturbance, stayed no longer than 10 days, and ...199989134
[comparative studies on the demonstrability of eosinophilic granuloma using biebrich's scarlet staining and adam's reaction]. 199985391
[control and prevention of foot and mouth disease in the western hemisphere]. 199985453
medical womanpower. 199989418
molecular-epigenetic aspects of phylo- and ontogenetic memory. 1998107470
protein-calorie malnutrition in patients with colorectal cancer.protein-calorie malnutrition, as determined by anthropometric measures and laboratory tests, was assessed in 17 preoperative patients with colorectal cancer and 47 control patients. the cancer patients had significant deficits in visceral protein and skeletal muscle and no deficit in fat stores. greater attention should be given to preoperative nutritional assessment and to the correction of protein deficits.1998104851
asymptomatic bacteriuria in pregnancy: epidemiological, clinical and microbiological approach.asymptomatic urinary tract infection is a risk factor for fetal and maternal morbidity including development of pyelonephritis, premature labor and impaired intra-uterine development. in this study, 326 pregnant and 100 non-pregnant control women were screened for significant asymptomatic bacteriuria from april 8 to july 25, 1997 to gain insight into the prevalence rate, clinical characteristics of the disease and microbiological assessments of the causative agents. all the subjects were clinica ...199810214459
antibacterial activity of peritoneal exudate in patients treated with 2 g cefotiam for surgical anti-microbial prophylaxis.the objective of this study was to investigate the presence of antibacterial activity in peritoneal exudate (pe) of patients treated with cefotiam (cft). cft (2 g) was administered as a 'single-shot' antimicrobial prophylaxis to 6 patients at the beginning of colorectal resection. samples of pe were collected from each patient on days 1, 2 and 3 after surgery. cft was detectable in the samples of day 1 for 5 of the 6 patients. the influence of pe on antibacterial activity of the antimicrobial dr ...19989612603
[bactericidal activity and capacity for selection of mutants resistant to imipenmen and meropenem in strains of proteus, morganella and providencia].to compare the bactericidal activity and frequency of mutation for meropenem and imipenem against proteus mirabilis, morganella morganii and providencia rettgeri.19989763741
studies on the formation of crystalline bacterial biofilms on urethral catheters.a model of the catheterised bladder was used to test the ability of urease-producing urinary tract pathogens to encrust urethral catheters. encrustation was assessed by determining the amounts of calcium and magnesium deposited on the catheters and visualised by scanning electron microscopy. urease-positive morganella morganii, klebsiella pneumoniae, and pseudomonas aeruginosa failed to raise the urinary ph and form crystalline biofilms. in contrast, strains of proteus mirabilis, proteus vulgari ...19989832268
natural antibiotic susceptibility of providencia stuartii, p. rettgeri, p. alcalifaciens and p. rustigianii strains.the natural antibiotic susceptibility of 38 providencia rettgeri, 35 p. stuartii, 23 p. alcalifaciens and 20 p. rustigianii strains was examined. mic values were determined by a microdilution procedure and evaluated by a table calculation programme. p. stuartii was the least susceptible providencia sp. and was naturally resistant to tetracyclines, some penicillins, older cephalosporins, sulphamethoxazole and fosfomycin and to antibiotics to which other species of enterobacteriaceae are also resi ...19989839568
antibacterial activity of human pleural fluid: alone and in combination with antibiotics.this study investigated the antibacterial activity of human pleural fluid (hpf) and its interaction with gentamicin (gm), meropenem (mrpm), ciprofloxacin (cpfx) and clarithromycin (cltm) against escherichia coli k-12, proteus rettgeri (sanelli) and staphylococcus aureus. minimal inhibitory concentrations or volumes, expressed as mic or volume percentage (miv, v/v%), were measured using a micro-dilution technique in microtiter plates. the antimicrobial activity of hpf combinations with antimicrob ...19989916908
adenocarcinoma of the prostate in perspective.adenocarcinoma of the prostate is responsible for one of every nine deaths from cancer in canada. in this review epidemiologic factors are considered and current staging systems are outlined. the american urological system is recommended for staging because of its ability to reflect changes in the understanding of the biologic behaviour of this neoplasm. the adoption of a quantitative grading scheme is suggested to complement the information obtained from the staging assessment. the routes of sp ...1998105800
controlled trial of disodium cromoglycate in chronic persistent ulcerative colitis.oral disodium cromoglycate (200 mg qds) has been tested in 26 patients with ulcerative colitis that was resistant to medical treatment. in a double-blind crossover trial disodium cromoglycate and placebo were added to conventional treatment in random order, each for four weeks. there was no significant difference in therapeutic effect between disodium cromoglycate and placebo.1998105970
[dental technical handling of palladium alloys]. 19983250889
enteral hyperalimentation: an alternative to central venous hyperalimentation.severe protein-energy undernutrition is a frequent finding among chronically ill patients. its causes are anorexia, hypermetabolism, and malabsorption. adverse consequences include impaired cell-mediated immunity increased susceptibility to infection, poor wound healing, weakness, and death. spontaneous oral intake is inadequate in patients with this disorder, and therapeutic maintenance or repletion alimentation is needed. enteral hyperalimentation is the method of choice, if tolerated. a succe ...1998105658
pharmaceutics: total parenteral nutrition in the cancer patient. 1998105802
[isolated aplastic anemia--a polyetiological syndrome]. 1998105498
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