Publications

[hospital and community-acquired β-lactamases-producing escherichia coli and klebsiella pneumoniae at hospitals in hermosillo, sonora].	to determine the prevalence of extended-spectrum β-lactamases (esbl)-producing esherichia coli and klebsiella pneumoniae in hospitals of hermosillo, sonora, mexico.	2011	21986791
[incidence, risk factors and outcome of nosocomial pneumonia patients with central nervous system infections].	pneumonia is the most frequent nosocomial infection in intensive care units. the reported frequency varies with definition, the type of hospital or intensive care units and the population of patients. the incidence ranges from 6.8-27%.	2011	21980657
engineering klebsiella sp. 601 multicopper oxidase enhances the catalytic efficiency towards phenolic substrates.	abstract: background: structural comparison between bacterial cueo and fungal laccases has suggested that a charged residue glu (e106) in cueo replaces the corresponding residue phe in fungal laccases at the gate of the tunnel connecting type ii copper to the protein surface and an extra alpha-helix (l351-g378) near the type i copper site covers the substrate binding pocket and might compromise the electron transfer from substrate to type i copper. to test this hypothesis, several mutants were m ...	2011	21624144
[comparative studies on the composition and antibiotic-resistance of pathogenic bacteria between children with community-acquired and hospital-acquired pneumonia.]	objective: this research was to explore the difference between children with community-acquired pneumonia (cap) and hospital-acquired pneumonia (hap) in the composition and antibiotic-resistance of pathogenic bacteria. methods: 241 cap and 116 hap with positive sputum culture who were hospitalized from january to december in 2008 in children's hospital affiliated to suzhou university were selected in this study. the bacteria were identified by traditionally manual method and antibiotic sensitivi ...	2011	21624231
[analysis of antibiotic resistance and genotypes on extended spectrum β-lactamase and ampc β-lactamase producing strains isolated from uygur and han newborns.]	objective: this study aimed to investigate drug resistance and genotypes of the extended spectrum β-lactamase (esbls) and ampc β-lactamase-producing escherichia coli and klebsiella pneumoniae isolated from uygur and han newborns in urumqi. methods: disk diffusion test (kirby-bauer) was used for detecting drug resistance of 299 strains to twenty two kinds of antibiotics. resistance genes of the esbls and ampc β-lactamase-producing strains were amplified by multiplex pcr and subtypes were confirme ...	2011	21624232
do mobile phones of patients, companions and visitors carry multidrug-resistant hospital pathogens?	a cross-sectional study was conducted to determine bacterial colonization on the mobile phones (mps) used by patients, patients' companions, visitors, and health care workers (hcws). significantly higher rates of pathogens (39.6% vs 20.6%, respectively; p = .02) were found in mps of patients' (n = 48) versus the hcws' (n = 12). there were also more multidrug pathogens in the patents' mps including methicillin-resistant staphylococcus aureus, extended-spectrum β-lactamase-producing escherichia co ...	2011	21624635
new delhi metallo-ss-lactamase-1: local acquisition in ontario, canada, and challenges in detection.	new delhi metallo-ß-lactamase-1 (ndm-1) is a recently identified metallo-ß-lactamase that confers resistance to carbapenems and all other ß-lactam antibiotics, with the exception of aztreonam. ndm-1 is also associated with resistance to many other classes of antibiotics. the enzyme was first identified in organisms isolated from a patient in sweden who had previously received medical treatment in india, but it is now recognized as endemic throughout india and pakistan and has spread worldwide. t ...	2011	21624908
molecular analysis of ndm-1-producing enterobacterial isolates from geneva, switzerland.	objectives to analyse the mechanisms responsible for decreased susceptibility or resistance to carbapenems in several enterobacterial isolates recovered in 2009-10 in geneva university hospitals, switzerland. methods pcr and sequencing were used to identify β-lactamases, 16s rna methylases and plasmid-mediated quinolone resistance genes. the transferable properties of the plasmids were analysed, as well as their plasmid type. the strains were typed by multilocus sequence typing. results three pa ...	2011	21628303
evaluations of bacterial contaminated full thickness burn wound healing in sprague dawley rats treated with tualang honey.	the effect of tualang honey on wound healing in bacterial contaminated full-thickness burn wounds was evaluated in 36 male sprague dawley rats.	2011	21713196
physicochemical and microbiological assessment of recreational and drinking waters.	the present study was aimed to make an assessment of health risk due to pollution and human pathogenic bacteria associated with the recreational and drinking water sources in twin densely populated holy indian cities ayodhya and faizabad. though physicochemical studies revealed that the water available in the area is under recommended limits for human use, it is unsafe on account of poor microbiological quality of surface and ground water in the region. the most probable number (mpn) test result ...	2011	21713494
kpc-2-producing klebsiella pneumoniae isolated from a czech patient previously hospitalized in greece and in vivo selection of colistin resistance.	carbapenemase-producing gram-negative bacteria peak clinical interest due to their ability to hydrolyze most +¦-lactams, including carbapenems; moreover, their genes spread through bacterial populations by horizontal transfer. bacteria with acquired carbapenemase have sporadically been reported in the czech republic, so far only in enterobacteriaceae and pseudomonas aeruginosa. in this study, we described the first finding of a kpc-2-producing strain of klebsiella pneumoniae, which was isolated ...	2011	21818609
antagonistic effect of lactobacillus strains against gas-producing coliforms isolated from colicky infants.	infantile colic is a common disturb within the first 3 months of life, nevertheless the pathogenesis is incompletely understood and treatment remains an open issue. intestinal gas production is thought to be one of the causes of abdominal discomfort in infants suffering from colic. however, data about the role of the amount of gas produced by infants' colonic microbiota and the correlation with the onset of colic symptoms are scanty. the benefit of supplementation with lactobacilli been recently ...	2011	21718486
klebsiella pneumoniae: development of a mixed population of carbapenem and tigecycline resistance during antimicrobial therapy in a kidney transplant patient.	clin microbiol infect abstract: nine isolates of klebsiella pneumoniae were isolated from a renal transplant patient suffering from recurrent urosepsis over a period of 4ôçâmonths. imipenem resistance was detected after imipenem-ertapenem therapy. when treatment was switched to tigecycline the k.-ápneumoniae developed resistance to tigecycline (micôçâ=ôçâ8ôçâmg/l). the nine isolates were tested by determination of agar dilution mics, phenotypic carbapenemase, extended-spectrum beta-lactamases an ...	2011	21722259
chemical composition and in vitro antibacterial activity of the essential oil of phthirusa adunca from venezuelan andes.	in this paper, preliminary studies on the chemical characterization of phthirusa adunca meyer essential oil, obtained by hydrodistillation, is presented. the separation of the components was performed by gc-ms. twenty-three compounds (94.5% of the sample) were identified of which the three major ones (76% of the sample) were beta-phellandrene (38.1%), germacrene d (26.8%) and beta-pinene (11.5%). the essential oil showed a broad spectrum of activity against salmonella typhi cdc 57 (100 microg/ml ...	2011	21834255
molecular characteristics of extended-spectrum +¦-lactamases in clinical isolates of enterobacteriaceae from the philippines.	+¦-lactamases, including extended-spectrum +¦-lactamases (esbls) and ampc +¦-lactamases, are major resistance mechanisms of enterobacteriaceae. emergence of plasmid-mediated quinolone resistance (pmqr) determinants in esbl-producing isolates poses a global threat. the molecular characterisitcs of esbl and pmqr determinants in the philippines are not well characterized. in this study, we investigated esbls and ampc +¦-lactamases in clinical isolates of enterobacteriaceae from the philippines, and ...	2011	21820398
bactericidal activity identified in 2s albumin from sesame seeds and in silico studies of structure-function relations.	pathogenic bacteria constitute an important cause of hospital-acquired infections. however, the misuse of available bactericidal agents has led to the appearance of antibiotic-resistant strains. thus, efforts to seek new antimicrobials with different action mechanisms would have an enormous impact. here, a novel antimicrobial protein (siamp2) belonging to the 2s albumin family was isolated from sesamum indicum kernels and evaluated against several bacteria and fungi. furthermore, in silico analy ...	2011	21691771
lateral buttock congenital dermal sinus tract.	a 6-month-old female presented with purulent discharge from a dimple in the right lateral buttock. a subcutaneous abscess was palpated on the right paravertebral region at the l5-s1 level. she had low-grade fever with laboratory findings of leukocytosis and elevation of c-reactive protein levels. klebsiella and enterococcus species were cultured from the pus. computed tomography (ct) clearly showed a tract traversing the subcutaneous tissue and connecting to the abscess. magnetic resonance (mr) ...	2011	21701114
the challenges of antimicrobial drug resistance in greece.	antimicrobial drug resistance rates in greece are among the highest in europe. the prevalence of carbapenem-resistant gram-negative species has increased considerably, including endemic strains in intensive care units. pandrug-resistant klebsiella pneumoniae, acinetobacter baumannii, and pseudomonas aeruginosa are sporadically reported. methicillin-resistant staphylococcus aureus and vancomycin-resistant enterococcus rates are also high in greek hospitals. multidrug resistance increases risk of ...	2011	21690626
potential role of tigecycline in the treatment of community-acquired bacterial pneumonia.	tigecycline is a member of the glycylcycline class of antimicrobials, which is structurally similar to the tetracycline class. it demonstrates potent in vitro activity against causative pathogens that are most frequently isolated in patients with community-acquired bacterial pneumonia (cabp), including (but not limited to) streptococcus pneumoniae (both penicillin-sensitive and -resistant strains), haemophilus influenzae and moraxella catarrhalis (including +¦-lactamase-producing strains), klebs ...	2011	21694911
rectal carriage of extended-spectrum beta-lactamase-producing gram-negative bacilli in community settings in madagascar.	extended-spectrum +ƒ-lactamase-producing enterobacteria (esbl-pe) emerged at the end of the 1980s, causing nosocomial outbreaks and/or hyperendemic situations in hospitals and long-term care facilities. in recent years, community-acquired infections due to esbl-pe have spread worldwide, especially across developing countries including madagascar.	2011	21829498
fecal carriage of serotype k1 klebsiella pneumoniae st23 strains closely related to liver abscess isolates in koreans living in korea.	we determined the fecal carriage rate of serotype k1 klebsiella pneumoniae in healthy koreans and studied their genetic relationship with liver abscess isolates. we compared the carriage according to the country of residence. the stool specimens were collected through health promotion programs in korea. k. pneumoniae strains were selected and tested for k1 by pcr. serotype k1 isolates were characterized by multilocus sequence typing and pulsed field gel electrophoresis. a total of 248ôçëk. pneum ...	2011	21739348
clinical and economic burden of community-acquired pneumonia amongst adults in the asia-pacific region.	community-acquired pneumonia (cap) is an important cause of mortality and morbidity amongst adults in the asia-pacific region. literature published between 1990 and may 2010 on the clinical and economic burden of cap amongst adults in this region was reviewed. cap is a significant health burden with significant economic impact in this region. chronic obstructive pulmonary disease, cardiovascular disease, diabetes mellitus and advanced age were risk factors for cap. aetiological agents included s ...	2011	21683553
pus in the ventricles of a patient with a lumbar cerebrospinal fluid drain for a thoracoabdominal aneurysm repair.		2011	21709541
antibiotic therapy in neonatal and pediatric septic shock.	severe sepsis accounts for nearly 4,500 deaths (mortality rate 10%), and is responsible for nearly $2 billion annual healthcare expenditure in the united states. early and speedy treatment of critically ill septic patients can halt or reduce the likelihood of physiologic progression to multi-system organ failure. a cornerstone of this therapeutic strategy is antibiotic administration. in this review, we discuss the empirical treatment strategies for the treatment of early and late neonatal sepsi ...	2011	21732046
bacterial contamination of saudi "one" riyal paper notes.	fomites are inanimate objects that are capable of absorbing, harboring and transmitting infectious microorganisms. paper currency, an exchangeable fomite, is constantly subjected to contamination. this study investigated bacterial contamination on the saudi one riyal paper note. two hundred one riyal bills (100 4th version and 100 5th version) were collected in the city of jeddah for examination. the notes were examined for bacterial contamination using blood agar and macconkey agar. eighty-eigh ...	2011	21706951
antimicrobial resistance patterns of uropathogens among children in istanbul, turkey.	urinary tract infections are a common cause of end-stage renal disease in turkey. this prospective study investigated the antibiotic resistance patterns of uropathogens in order to recommend appropriate therapeutic protocols for children with urinary tract infections in istanbul, turkey. between october 2007 and october 2008, children presenting with a first episode of urinary tract infection to a pediatric outpatient clinic were enrolled in the study. urine samples were cultured, and antimicrob ...	2011	21710858
[russian results of the international epidemiological study of clinical aspects and resistance to antimicrobial medicines of cystitis uropathogens in females (aresc): significance of empiric therapy].	a total of 656 female patients from russia aged between 18 to 65 years with uncomplicated cystitis entered the international aresc trial, of them 647 were eligible for final analysis. positive cultural urine tests ( > 10(4) cfu/ml) were in 419 (64.7%) patients, 393 (93.8%) patients had monoinfection. the central laboratory of genoa (to which the isolated samples were sent) has tested 416 uropathogens from 399 patients. the following pathogens were isolated: e. coli (72.6%), enterococcus (7.0%), ...	2011	21815455
emerging severe and fatal infections due to klebsiella pneumoniae in two university hospitals in france.	severe infections caused by hypermucoviscous klebsiella pneumoniae have been reported in southeast asian countries over the past several decades. this report shows their emergence in france, with 12 cases observed during a 2-year period in two university hospitals. two clones (sequence type 86 [st86] and st380) of serotype k2 caused five rapidly fatal bacteremia cases, three of which were associated with pneumonia, whereas seven liver abscess cases were caused by k1 strains of st23.	2011	21677064
lytic myophage abp53 encodes several proteins similar to those encoded by host acinetobacter baumannii and phage phiko2.	acinetobacter baumannii is an important gram-negative opportunistic pathogen causing nosocomial infections. the emergence of multiple drug resistant a. baumannii isolates has increased in recent years. directed towards phage therapy, a lytic phage of a. baumannii, designated abp53, was isolated from a sputum sample in this study. abp53 has an isometric head and a contractile tail with tail fibers, belonging to myoviridae, a latent period of about 10 min, and a burst size of approximately 150 pfu ...	2011	21821767
comparison of ctx-m-14- and ctx-m-15-producing escherichia coli and klebsiella pneumoniae isolates from patients with bacteremia.	recently, ctx-m-15-producing enterobacteriaceae has disseminated worldwide. to better understand the success of ctx-m-15-type extended-spectrum +¦-lactamase, we compared the ctx-m-15-producing escherichia coli and klebsiella pneumoniae isolates with ctx-m-14-producing e. coli and k. pneumoniae isolates that had been more prevalent before the recent increase of ctx-m-15 in korea.	2011	21712135
outcomes and genetic relatedness of carbapenem-resistant enterobacteriaceae at detroit medical center.	background.ôçâcarbapenem-resistant enterobacteriaceae (cre) are rapidly emerging in hospitals in the united states and are posing a significant threat. to better understand the transmission dynamics and the acquisition of resistant strains, a thorough analysis of epidemiologic and molecular characteristics was performed. methods.ôçâcre isolated at detroit medical center were analyzed from september 2008 to september 2009. bla(kpc) genes were investigated by polymerase chain reaction (pcr), and r ...	2011	21828966
establishment of the first international repository for transfusion-relevant bacteria reference strains: isbt working party transfusion-transmitted infectious diseases (wp-ttid), subgroup on bacteria.	backgroundôçé bacterial contamination of platelet concentrates (pcs) still remains a significant problem in transfusion with potential important clinical consequences, including death. the international society of blood transfusion working party on transfusion-transmitted infectious diseases, subgroup on bacteria, organised an international study on transfusion-relevant bacteria references to be used as a tool for development, validation and comparison of both bacterial screening and pathogen re ...	2011	21732948
an optimized method for suicide vector-based allelic exchange in klebsiella pneumoniae.	klebsiella pneumoniae is an important and versatile bacterium that can be found in diverse environments and is also a frequent cause of human infections. limited data exists on the mechanisms of interaction between k. pneumoniae and the human host and of adaptations to other environments. coupled with the high genetic diversity of this species, these factors highlight the necessity for substantial further k. pneumoniae-focused molecular genetics studies. in this report we describe a simple and e ...	2011	21699924
a novel complex class 1 integron found in a klebsiella pneumoniae isolate from portugal.	klebsiella pneumoniae kp1 carrying a novel complex class 1 integron was isolated from an inanimate surface of a female ward sanitary facility in the hospital infante d. pedro, aveiro, central portugal. the integron consists of two variable regions (vrs); vr1 was previously described in escherichia coli and vibrio cholerae, and vr2 contains an in37-like structure and is located downstream of an iscr1 element. the integron was found on a plasmid of 225 kb. the qnrb10 gene, although present, is not ...	2011	21722254
dosing regimens of oral ciprofloxacin for children with severe malnutrition: a population pharmacokinetic study with monte carlo simulation.	background severe malnutrition is frequently complicated by sepsis, leading to high case fatality. oral ciprofloxacin is a potential alternative to the standard parenteral ampicillin/gentamicin combination, but its pharmacokinetics in malnourished children is unknown. methods ciprofloxacin (10 mg/kg, 12 hourly) was administered either 2 h before or up to 2 h after feeds to kenyan children hospitalized with severe malnutrition. four plasma ciprofloxacin concentrations were measured over 24 h. pop ...	2011	21831986
genetic evolution and clinical impact in extended-spectrum +¦-lactamase-producing escherichia coli and klebsiella pneumoniae.	the emergence of extended-spectrum +¦-lactamase (esbl)-producing bacteria, particularly escherichia coli and klebsiella pneumoniae, is now a critical concern for the development of therapies against bacterial infection. esbls consist of three major genetic groups: tem, shv, and ctx-m types. nosocomial infections due to tem and shv-producing k. pneumoniae strains were frequently documented until the late 1990s. the number of reports on community-acquired infections caused by ctx-m-producing e. co ...	2011	21689785
results of urine culture and antimicrobial sensitivity tests according to the voiding method over 10 years in patients with spinal cord injury.	we studied the results of urine cultures and antimicrobial sensitivity tests according to the voiding method used by spinal cord injury (sci) patients over a recent 10-year period.	2011	21687395
molecular analysis of a prolonged spread of klebsiella pneumoniae co-producing dha-1 and shv-12 +¦-lactamases.	the study investigated molecular mechanisms for prolonged nosocomial spread of multidrug-resistant klebsiella pneumoniae co-producing plasmid-mediated ampc +¦-lactamase dha-1 and extended-spectrum +¦-lactamase shv-12. forty-eight clinical isolates of k. pneumonia, resistant to the extended-spectrum cepha-losporins, were collected in a 750-bed university hospital over a year. the isolates were characterized for pcr-based +¦-lactamase genotypes, isoelectric focusing and pulsed-field gel electropho ...	2011	21717319
real-time detection of blakpc in clinical samples and surveillance specimens.	a real-time pcr assay was developed targeting the bla(kpc) responsible for klebsiella pneumoniae carbapenemase (kpc)-mediated carbapenem resistance and was validated for testing colonies or enrichment broth cultures. the assay accurately detects kpc-containing strains with high analytical specificity and sensitivity.	2011	21734030
colonization of the gut with gram negative bacilli, its association with neonatal sepsis and its clinical relevance in a developing country.	this study examines the pattern of colonization of the neonatal gut by aerobic gram negative bacilli (gnb) and evaluates the association between gut colonization and sepsis in the developing world. this deserves attention because of the high incidence of sepsis and the differences in hygienic environment in developing countries compared to the developed world. the study was carried out on neonates in a tertiary care government hospital. the serial gut samples were analyzed (gastric aspirate, ga- ...	2011	21719575
factors associated with the quality of well water and the prevalence of waterborne diseases in the municipality of abomey-calavi in benin.	the objective of this work is to assess the factors associated with the water quality of household wells, suggest solutions to improve it, and study the prevalence of water-borne diseases in this community. the quality of well water and the prevalence of waterborne diseases in the municipality were studied by analysis of the microbiological indicators currently used to assess drinking-water quality and the retrospective study of waterborne diseases treated in the local health centres. the wells ...	2011	21700557
changes in bacterial resistance patterns in children with urinary tract infections on antimicrobial prophylaxis at university hospital in split.	we assessed prevalence and resistance of uropathogens on antimicrobial agents (aa) from urine cultures (uc) in children hospitalized with urinary tract infections (uti) at university hospital in split.	2011	21709628
in vitro potency of various polymyxin b components.		2011	21709096
detection of volatile compounds produced by microbial growth in urine by selected ion flow tube mass spectrometry (sift-ms).	selected ion flow tube-mass spectrometry has been used to measure the volatile compounds occurring in the headspace of urine samples inoculated with common urinary tract infection (uti)-causing microbes escherichia coli, proteus vulgaris, pseudomonas aeruginosa, staphylococcus aureus, staphylococcus epidermidis, klebsiella pneumoniae, enterococcus faecalis, or candida albicans. this technique has the potential to offer rapid and simple diagnosis of the causative agent of utis.	2011	21741416
arthritis, osteomyelitis, septicemia and meningitis caused by klebsiella in a low-birth-weight newborn: a case report.	abstract:	2011	21707974
investigation of the antimicrobial activity of rhaponticum (rhaponticum carthamoides d.c. iljin) and shrubby cinquefoil (potentilla fruticosa l.).	the aim of the study was to determine antimicrobial activity of rhaponticum and shrubby cinquefoil extracts. material and methods. ethanol extract from the leaves of rhaponticum (rhaponticum carthamoides d.c. iljin) and shrubby cinquefoil (potentilla fruticosa l.) was produced at the department of food technology, kaunas university of technology. the antimicrobial activity of the viscous extract or rhaponticum and shrubby cinquefoil was evaluated using standard microorganism cultures (bacteria s ...	2011	21822040
novel variants of the qnrb gene, qnrb31 and qnrb32, in klebsiella pneumoniae.	quinolone resistance in the enterobacteriaceae families is mostly attributed to the accumulation of mutations in the bacterial enzymes targeted by fluoroquinolones: dna gyrase and dna topoisomerase iv. here we isolated the strains kp3606 and kp4707 in klebsiella pneumoniae in different specimens from 2008 to 2010 in taizhou municipal hospital of china, discovered a new subtype qnrb31, which genbank accession number is hq418999, and another new subtype qnrb32, which genbank accession number is hq ...	2011	21816942
does it ever make sense to treat a bacterial infection with a drug against which the responsible pathogen possesses a resistance mechanism?		2011	21689208
Identification and Characterization of OXA-48 Producing, Carbapenem-Resistant Enterobacteriaceae Isolates in Turkey.	Carbapenem resistance in Enterobacteriaceae isolates has been reported from Turkey and is most often mediated by OXA-48 type carbapenemases. We report the identification and characterization of four carbapenem-resistant isolates (three Klebsiella pneumoniae and one Escherichia coli) among 515 clinical Enterobacteriaceae isolates collected during a 7-month study period in Ankara, Turkey. The four isolates were recovered from blood and urine specimens in patients with varied clinical manifestation ...	2011	21844575
outcome of carbapenem resistant klebsiella pneumoniae bloodstream infections.	the aim of this study was to evaluate the impact of carbapenem-resistant k. pneumoniae bloodstream infections on mortality. during the study period 42, 68 and 120 patients were identified with carbapenem-resistant, extended-spectrum β-lactamase producers (esbl) and susceptible k. pneumoniae bloodstream infections, respectively. patients with carbapenem-resistant k. pneumoniae had higher rates of prior antimicrobial exposure, other nosocomial infections, and use of invasive devices. infection-rel ...	2011	21722257
untreated primary thyroid lymphoma in an elderly woman.		2011	21727773
[microbiology of surgical site infections in abdominal tract surgery patients].	knowledge of the microbiology of surgical infections after abdominal surgery can be of use when prescribing effective empirical antibiotic treatments.	2011	21820107
antisense inhibition of gene expression and growth in gram-negative bacteria by cell-penetrating peptide conjugates of peptide nucleic acids targeted to rpod gene.	gram-negative bacteria (gnb) cause common and severe hospital- and community-acquired infections with a high incidence of multidrug resistance (mdr) and mortality. the emergence and spread of mdr-gnb strains limit therapeutic options and highlight the need to develop new therapeutic strategies. in this study, the peptide (rxr)(4)xb- and (kff)(3)k-conjugated peptide nucleic acids (ppnas) were developed to target rpod, which encodes an rna polymerase primary σ(70) that is thought to be essential f ...	2011	22000398
enhanced reducing equivalent generation for 1,3-propanediol production through cofermentation of glycerol and xylose by klebsiella pneumoniae.	1,3-propanediol (1,3-pd) biosynthesis plays a key role in nadh consumption to regulate the intracellular reducing equivalent balance of klebsiella pneumoniae. this study aimed to increase reducing equivalent for enhancing 1,3-pd production through cofermentation of glycerol and xylose. adding xylose as cosubstrate resulted in more reducing equivalent generation and higher cell growth. in batch fermentation under microaerobic condition, the 1,3-pd concentration, conversion from glycerol, and biom ...	2011	21960271
quantification and analysis of airborne bacterial characteristics in a nursing care institution.	indoor air quality has become a critical issue because people spend most of their time in the indoor environment. the factors that influence indoor air quality are very important to environmental sanitation and air quality improvement. this study focuses on monitoring air quality, colony counts, and bacteria species of the indoor air of a nursing care institution. the regular colony counts in two different wards range from 55 to 600 cfu m(-3) regression analysis results indicate that the bacteri ...	2011	21850827
seasonal and temperature-associated increases in gram-negative bacterial bloodstream infections among hospitalized patients.	knowledge of seasonal trends in hospital-associated infection incidence may improve surveillance and help guide the design and evaluation of infection prevention interventions. we estimated seasonal variation in the frequencies of inpatient bloodstream infections (bsis) caused by common bacterial pathogens and examined associations of monthly bsi frequencies with ambient outdoor temperature, precipitation, and humidity levels.	2011	21966489
use of ceramic-based cell immobilization to produce 1,3-propanediol from biodiesel-derived waste glycerol with klebsiella pneumoniae.	the feasibility of the continuous production of a valuable bioplastic raw material, namely 1,3-propanediol (1,3-pdo) from biodiesel by-product glycerol, using immobilized cells was investigated. in addition, the effect of hydraulic retention time (hrt) was also analysed.	2011	21883732
influence of blocking of 2,3-butanediol pathway on glycerol metabolism for 1,3-propanediol production by klebsiella oxytoca.	glycerol metabolism is a typical biological oxidoreductive reaction. 1,3-propanediol (1,3-pd) is the final product of the reductive branch, while acetate, succinate, lactate, 2,3-butanediol (2,3-bd), and ethanol were produced in the oxidative branch. 2,3-bd, which has similar properties of high boiling point and water solubility with 1,3-pd, not only contests the carbon flow and nadh with 1,3-pd but also serves as an obstacle for obtaining high purity 1,3-pd in downstream processes. in this stud ...	2011	21915590
production of (2s,3s)-2,3-butanediol and (3s)-acetoin from glucose using resting cells of klebsiella pneumonia and bacillus subtilis.	production of highly pure (2s,3s)-2,3-butanediol ((2s,3s)-2,3-bd) and (3s)-acetoin ((3s)-ac) in high concentrations is desirable but difficult to achieve. in the present study, glucose was first transformed to a mixture of (2s,3s)-2,3-bd and meso-2,3-bd by resting cells of klebsiella pneumoniae cicc 10011, followed by biocatalytic resolution of the mixture by resting cells of bacillus subtilis 168. meso-2,3-bd was transformed to (3s)-ac, leaving (2s,3s)-2,3-bd in the reaction medium. using this ...	2011	21945208
Prevalence and characterization of extended spectrum ß-lactamases in Klebsiella-Enterobacter-Serratia group bacteria, in Algeria.	OBJECTIVES: The authors had for aim to assess the local epidemiology, antibiotic resistance, and molecular typing of expanded spectrum betalactamase producing Klebsiella, Enterobacter, and Serratia (ESBL KES). MATERIALS AND METHODS: Two hundred and seven strains of the KES group were isolated in the microbiology laboratory of the Annaba Ibn Rochd hospital in 2009. The antibiotic resistance (diffusion method and MIC) was tested and ESBL detection was performed as recommended by the Clinical Labor ...	2011	22056377
partial excision of blakpc from tn4401 in carbapenem-resistant klebsiella pneumoniae.	we describe a novel tn4401 variant (tn4401d) in epidemic klebsiella pneumoniae clone st258, from which a partial bla(kpc) fragment has been excised along with iskpn7 and a partial tnpa fragment. nested pcr experiments confirmed that this region can be removed from distinct tn4401 isoforms in both k. pneumoniae and escherichia coli. this study highlights that the region surrounding bla(kpc) is undergoing recombination, and that tn4401 itself is heterogeneous and highly plastic.	2011	22203593
an effective intervention to limit the spread of an epidemic carbapenem-resistant klebsiella pneumoniae strain in an acute care setting: from theory to practice.	the highly transmissible and virulent carbapenem-resistant klebsiella pneumoniae (crkp) kpc-3 strain has been spreading in our medical center and in other centers in israel since 2006. an intervention that aimed to diminish its prevalence was constructed and applied in our institute.	2011	21864942
reduction of molybdate to molybdenum blue by klebsiella sp. strain hkeem.	a novel molybdate-reducing bacterium, tentatively identified as klebsiella sp. strain hkeem and based on partial 16s rdna gene sequencing and phylogenetic analysis, has been isolated. strain hkeem produced 3 times more molybdenum blue than serratia sp. strain dr.y8; the most potent mo-reducing bacterium isolated to date. molybdate was optimally reduced to molybdenum blue using 4.5 mm phosphate, 80 mm molybdate and using 1% (w/v) fructose as a carbon source. molybdate reduction was optimum at 30 ...	2011	22052341
In vitro analysis of the bactericidal activity of Escherichia coli Nissle 1917 against pediatric uropathogens.	The usefulness of prophylactic antibiotics to prevent recurrent urinary tract infections in children was recently questioned. Some groups have attempted to use probiotics, most commonly lactobacillus, to prevent recurrent infections by altering the intestinal bacterial reservoir with variable results. Mutaflor® is a possible alternative probiotic in which the active agent is Nissle 1917. Nissle 1917 is a commensal Escherichia coli strain that eradicates pathogenic bacteria from the gastrointesti ...	2011	21855931
the antimicrobial efficacy of silver on antibiotic-resistant bacteria isolated from burn wounds.	the antibiotic-resistant bacteria are a major concern to wound care because of their ability to resist many of the antibiotics used today to treat infections. consequently, other antimicrobials, in particular ionic silver, are considered ideal topical agents for effectively helping to manage and prevent local infections. little is known about the antimicrobial efficacy of ionic silver on antibiotic-resistant bacteria at different ph values. consequently, in this study our aim was to evaluate the ...	2011	22182219
production of a novel bioflocculant mnxy1 by klebsiella pneumoniae strain ny1 and application in precipitation of cyanobacteria and municipal wastewater treatment.	aims:ôçé to isolate and characterize the novel bioflocculant-producing bacteria, to optimize the bioflocculant production and to evaluate its potential applications. methods and results:ôçé klebsiella pneumoniae strain ny1, a bacterium that produces a novel bioflocculant (mnxy1), was selected on the chemically defined media. it was classified according to the 16s rrna gene sequence, morphological and microscopic characteristics. mnxy1 was characterized to contain 26% protein and 66% total sugar. ...	2011	21679283
liver cirrhosis as a risk factor for mortality in a national cohort of patients with bacteremia.	objective: the purpose of this study was to evaluate clinical features and outcomes of bacteremia in patients with liver cirrhosis (lc) and determine whether underlying lc is an independent risk factor for mortality in a population of patients with different underlying diseases. methods: from the database of nationwide surveillance studies for bacteremia, data regarding bacteremia in patients with lc were analyzed and compared with those in patients with other diseases. results: a total of 195 p ...	2011	21835195
inflammation restraining effects of prostaglandin e2 on natural killer-dendritic cell (nk-dc) interaction are imprinted during dc maturation.	among prostaglandins (pgs), pge2 is abundantly expressed in various malignancies and is probably one of many factors promoting tumor growth by inhibiting tumor immune surveillance(.) in the current study, we report on a novel mechanism by which pge2 inhibits in vitro natural killer-dendritic cell (nk-dc) crosstalk and thereby innate and adaptive immune responses via its effect on nk-dc crosstalk. the presence of pge2 during ifn-+¦/membrane fraction of klebsiella pneumoniae dc maturation inhibits ...	2011	21715307
macrophage genetic reprogramming during chronic peritonitis is augmented by lps pretreatment.	background: persistent and tertiary chronic peritonitis is a clinically challenging problem especially in those who are critically ill. this could be attributed to a state of immune-paralysis, known as microbial tolerance. microbial tolerance is the diminished pro-inflammatory protein response following repeated stimulation by numerous pathogen-associated molecular patterns (pamps) of varying origins, which we have shown in this novel model of chronic peritonitis. we aimed in this study to inves ...	2011	21696761
antimicrobial resistance in pathogens causing nosocomial bloodstream infections in university hospitals in egypt.	background: nosocomial bloodstream infections (bsis) and antimicrobial resistance (amr) are worldwide health care problems causing substantial patient morbidity and mortality. this study was conducted to identify bacterial pathogens isolated from nosocomial bsis and determine their amr patterns. methods: an active surveillance program for bsis was conducted in intensive care units in 3 large university hospitals in egypt between september 1, 2006, and june 30, 2007. infection prevention and cont ...	2011	21835504
mucosal surveillance cultures in predicting gram-negative late-onset sepsis in neonatal intensive care units.	this study aimed to examine the spectrum and time course of gut and nasopharyngeal colonization with gram-negative micro-organisms, and to define the value of surveillance cultures in predicting late-onset sepsis in neonates admitted to neonatal intensive care units. nasopharyngeal and rectal swabs were collected on admission and twice weekly thereafter in 278 neonates admitted within the first 72-áh of life with risk factors of early-onset sepsis. sterile body fluid cultures were obtained on ad ...	2011	21684633
usefulness of c-reactive protein for evaluating clinical outcomes in cirrhotic patients with bacteremia.	the purpose of this study was to evaluate the value of initial c-reactive protein (crp) as a predictor of clinical outcome and to investigate whether follow-up crp measurement is useful for the prediction of the clinical outcome of bloodstream infections in patients with liver cirrhosis (lc), whose crp production in response to infection may be attenuated.	2011	21716910
impact of a clonal outbreak of extended-spectrum {beta}-lactamase-producing klebsiella pneumoniae in the development and evolution of bloodstream infections by k. pneumoniae and escherichia coli: an 11 year experience in oxfordshire, uk.	objectives the objectives of this study were: (i) to describe an outbreak of multidrug-resistant klebsiella pneumoniae in our population; (ii) to identify the potential source of this outbreak by examining antibiotic resistance trends in urocultures; (iii) to evaluate the contribution of this outbreak to resistance patterns over time in the two commonest gram-negative blood culture isolates, namely k. pneumoniae and escherichia coli; and (iv) to assess risk factors for multidrug resistance and t ...	2011	21693458
Impact of solvent/detergent treatment of plasma on transfusion-relevant bacteria.	Background A solvent/detergent (S/D) treatment in a medical device has been developed for pathogen reduction of plasma for transfusion. Impact of S/D on bacterial growth and on the capacity of complement to kill bacteria has been investigated in this study. Study design and methods A pool of apheresis plasma from four donors was spiked with eight transfusion-relevant bacteria. Plasma was treated with 1% tri(n-butyl) phosphate and 1% Triton X-45 at 31°C for 90 min and then extracted by oil at 3 ...	2011	22092109
characteristics of healthcare-associated and community-acquired klebsiella pneumoniae bacteremia in taiwan.	klebsiella pneumoniae is the major cause of community-onset pyogenic infections in taiwan. we investigated the clinical features and outcomes of community-acquired (ca) and healthcare-associated (hca) infections among community-onset k. pneumoniae bacteremia.	2011	22101080
Bloodstream infections in adult patients with cancer: clinical features and pathogenic significance of Staphylococcus aureus bacteremia.	OBJECTIVES: The aim of this study was to more precisely delineate the characteristics and outcomes of bloodstream infections in adult cancer patients. METHODS: Using a database for nationwide surveillance of bacteremia, we analyzed data related to bacteremia in adult patients with cancer in order to evaluate clinical features and outcomes and to define predictive factors for mortality. ...	2011	22193772
vim and imp metallo-β-lactamases and other extended-spectrum β-lactamases in escherichia coli and klebsiella pneumoniae from environmental samples in a tunisian hospital.	an extremely drug-resistant enterobacteriaceae species emerged in kasserine hospital, tunisia between 2009 and 2010 causing a local outbreak. we aimed to characterize extended-spectrum β-lactamase (esbl) and metallo-β-lactamase (mbl)-producing enterobacteriaceae from the hospital environment. swabs were collected from ten different wards from kasserine hospital, tunisia. a total of 46 isolates were cultured onto macconkey agar supplemented with ceftazidime to select for esbl-producing enterobact ...	2011	21917010
[Aspects of the antimicrobial resistence profile in infections with Escherichia coli and Klebsiella pneumoniae in diabetic patients].	Infections in diabetic patient remains an important cause of morbidity and mortality, triggering and maintaining a prolonged metabolic imbalance. Emergence of extented spectrum beta-lactmase (ESBL) in Escherichia coli and Klebsiella pneumoniae is a major concern, because of the atypical manner infection acts in this group of imunodepressed patients and also for the limited therapeutic solutions. For this reason we have evaluated the profile of antimicrobial resistance of these pathogens in both ...	2011	22046785
The optimization of biohydrogen production by bacteria using residual glycerol from biodiesel synthesis.	In this research the production of hydrogen by Klebsiella pneumoniae BLb01 using residual glycerol discharged from a biodiesel fuel production plant was investigated. Klebsiella pneumoniae BLb01 was isolated from a bacteria-rich sludge of an upflow anaerobic sludge blanket reactor (UASB) of a soybean processing plant. A Plackett-Burman design (P-B) and Response Surface Methodology (RSM) were employed to determine the optimal condition for enhanced hydrogen production. The maximal hydrogen produc ...	2011	21967246
Bacterial nitrate assimilation: gene distribution and regulation.	In the context of the global nitrogen cycle, the importance of inorganic nitrate for the nutrition and growth of marine and freshwater autotrophic phytoplankton has long been recognized. In contrast, the utilization of nitrate by heterotrophic bacteria has historically received less attention because the primary role of these organisms has classically been considered to be the decomposition and mineralization of dissolved and particulate organic nitrogen. In the pre-genome sequence era, it was k ...	2011	22103536
Clonal dissemination of Klebsiella pneumoniae ST258 harbouring KPC-2 in Argentina.	The present work describes the abrupt emergence of Klebsiella pneumoniae carbapenemase (KPC) and characterizes the first 79 KPC-producing enterobacteria from Argentina (isolated from 2006 to 2010). The emergence of bla(KPC-2) was characterized by two patterns of dispersion: the first was the sporadic occurrence in diverse enterobacteria from distant geographical regions, harbouring plasmids of different incompatibility groups and bla(KPC-2) in an unusual genetic environment flanked by ISKpn8-?bl ...	2011	21851480
Characterization of the inhibitor-resistant SHV ß-lactamase (SHV-107) in a clinical Klebsiella pneumoniae strain co-producing GES-7 enzyme.	The clinical K. pneumoniae INSRA6884 strain exhibited non-susceptibility to all penicillins tested (MICs of 64 to >2048 µg/ml). The MICs of penicillins were weakly reduced by clavulanate (from 2048 to 512 µg/ml), and tazobactam restored piperacillin susceptibility. Molecular characterization identified the genes bla(GES-7) and a new ß-lactamase gene bla(SHV-107), which encoded an enzyme that differed from SHV-1 by the amino acid substitutions Leu35Gln and Thr235Ala. The SHV-107-producing E. coli ...	2011	22083476
Risk factors for bloodstream infections caused by extended-spectrum ß-lactamase-producing Escherichia coli and Klebsiella pneumoniae.	The objective of this study was to identify risk factors for bacteremia by extended-spectrum ß-lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae. Retrospective case-control study performed in a 450-bed acute care academic tertiary hospital in Barcelona, Spain. Cases included 53 patients with ESBL-producing E. coli or K. pneumoniae bacteremia, and 159 controls with non-ESBL-producing E. coli or K. pneumoniae bacteremia. Controls were matched in a 3:1 ratio to case patients acc ...	2011	21861009
Development of an antibiogram in a long-term care facility.	To create an antibiogram-a profile of an organism's susceptibility/resistance to a panel of antibiotics- for a long-term care facility to assess the prevalence of resistance of bacteria present at the facility.	2011	22079792
Antimicrobial susceptibility of Gram-negative organisms from intraabdominal infections and evolution of isolates with extended spectrum ß-lactamases in the SMART study in Spain (2002-2010).	Introduction. The SMART (Study for Monitoring Antimicrobial Resistance Trends) surveillance study records the antimicrobial susceptibility of Gram-negative bacilli obtain from intraabdominal infections with special focus in isolates with extended spectrum ß-lactamases (ESBLs). Material and Methods. The antimicrobial susceptibility of 8,869 isolates was analyzed by microdilution during the SMART study performed in Spain from 2002 to 2010. Isolates were recovered in 16 centres. Results. Escherichi ...	2011	22173194
successful treatment of ndm-1 klebsiella pneumoniae bacteraemia in a neutropenic patient.	the rapid emergence of novel multidrug-resistant organisms coupled with the slow development of new antibiotics is of great concern. with the discovery of new delhi metallo-beta-lactamase 1 (ndm-1)-producing klebsiella pneumoniae, clinicians are faced with problems in the treatment of infections caused by this multidrug-resistant organism. therapeutic experiences are limited. we share our experience of the successful treatment of a febrile neutropenic patient with ndm-1-producing k. pneumoniae b ...	2011	22126430
oral levofloxacin brings better results in treating endogenous klebsiella pneumoniae endophthalmitis.		2011	22149484
pcr-based identification of klebsiella pneumoniae subsp. rhinoscleromatis, the agent of rhinoscleroma.	rhinoscleroma is a chronic granulomatous infection of the upper airways caused by the bacterium klebsiella pneumoniae subsp. rhinoscleromatis. the disease is endemic in tropical and subtropical areas, but its diagnosis remains difficult. as a consequence, and despite available antibiotherapy, some patients evolve advanced stages that can lead to disfiguration, severe respiratory impairment and death by anoxia. because identification of the etiologic agent is crucial for the definitive diagnosis ...	2011	21629720
comparative evaluation of boronic acid combined-disk tests for the detection of kpc carbapenemase-producing enterobacteriaceae clinical isolates.	the accurate phenotypic detection of kpc-producing enterobacteriaceae is an increasing necessity worldwide. we evaluated the performance of boronic acid combined-disk tests using as substrate impenem or meropenem and as inhibitor of kpc production 300 μg aminophenylboronic acid (apba), 600 μg apba or 400 μg phenylboronic acid (pba). tests were considered positive when an increase in the growth-inhibitory zone around carbapenem disk with kpc inhibitor was 5 mm or greater of the growth-inhibitory ...	2011	21632901
bacteriology of deep neck abscesses: a retrospective review of 96 consecutive cases.	introduction: this study aimed to review the microbiology of deep neck abscesses and identify the factors that influence their occurrence. methods: a retrospective chart review was done of patients diagnosed with deep neck abscesses at the department of otorhinolaryngology, tan tock seng hospital, singapore between 2004 and 2009. the data of 131 deep neck abscess patients were reviewed, and those with positive pus culture were included in the study. logistic regression was applied to analyse and ...	2011	21633769
[effect of heavy metal ions on triphenyltin enzymatic degradation].	the influence of different metal ions and different forms of addition on triphenyltin enzymatic degradation was investigated under conditions using enzyme obtained from klebsiella pneumoniae. the objective of this study is to illuminate the mechanism of enzymatic degradation of triphenyltin (tpht). the results demonstrated that the strain was able to tolerate k+, mg2+, cu2+, ca2+ and fe3+ at high concentrations. high concentrations of zn2+ and fe2+ had some toxic effects on the strain, thus affe ...	2011	21634178
carbapenemase-producing klebsiella pneumoniae: (when) might we still consider treating with carbapenems?	clin microbiol infect 2011; 00: 000-000 abstract: infections caused by carbapenemase-producing klebsiella pneumoniae (cpkp) are increasing in frequency worldwide. cpkp isolates exhibit extensive drug resistance phenotypes, complicate therapy, and limit treatment options. although cpkp isolates are often highly resistant to carbapenems, a proportion of these have relatively low mics for carbapenems, raising the question of whether this class of agents has any therapeutic potential against cpkp in ...	2011	21635663
studies on interaction of colloidal silver nanoparticles (snps) with five different bacterial species.	silver nanoparticles (snps) are being increasingly used in many consumer products like textile fabrics, cosmetics, washing machines, food and drug products owing to its excellent antimicrobial properties. here we have studied the adsorption and toxicity of snps on bacterial species such as pseudomonas aeruginosa, micrococcus luteus, bacillus subtilis, bacillus barbaricus and klebsiella pneumoniae. the influence of zeta potential on the adsorption of snps on bacterial cell surface was investigate ...	2011	21640562
klebsiella pneumoniae producing kpc carbapenemase in a district general hospital in the uk.	we report two patients with multidrug-resistant kpc-carbapenemase-producing klebsiella pneumoniae urinary tract infections. a bla(kpc-2) gene was detected in both of the isolates by polymerase chain reaction and sequencing. the isolates had identical pulsed-field gel electrophoresis patterns and belonged to sequence type st11. the index patient probably acquired the kpc-producing strain while in hospital in curaçao, with subsequent nosocomial transmission to the second patient occurring in our h ...	2011	21641083
assessing the effect of oxygen and microbial inhibitors to optimize ferricyanide-mediated bod assay.	methods for short-term bod analysis (bod(st)) based on ferricyanide mediator reduction have succeeded in overcoming some problems associated with the standard bod test analysis (bod(5)) such as long-term incubations (5 days), the need to dilute samples and low reproducibility. here we present a bioassay where a klebsiella pneumoniae environmental strain successfully reduces ferricyanide without de-aeration of the samples with linear bod(5) ranges between 30 and 500mgl(-1) or 30 and 200mgl(-1), u ...	2011	21645725
biofilm formation as a function of adhesin, growth medium, substratum and strain type.	biofilm formation is involved in the majority of bacterial infections. comparing six escherichia coli and klebsiella pneumoniae isolates revealed significant differences in biofilm formation depending on the growth medium. fimbriae are known to be involved in biofilm formation, and type 1, f1c and p fimbriae were seen to influence biofilm formation significantly different depending on strain background, growth media and aeration as well as surface material. altogether, this report clearly demons ...	2011	21646046
expression of a clostridium perfringens type iv pilin by neisseria gonorrhoeae mediates adherence to muscle cells.	clostridium perfringens is an anaerobic, gram-positive bacterium that causes a range of diseases in humans, including lethal gas gangrene. we have recently shown that strains of c. perfringens move across the surface of agar plates using a unique type iv pili (tfp)-mediated social motility that had not been previously described. based on sequence homology to pilins in gram-negative bacteria, c. perfringens appears to have two pilin subunits, pila1 and pila2. structural prediction analysis indica ...	2011	21646450
asymptomatic bacteriuria and antibacterial susceptibility patterns in an obstetric population.	introduction. asymptomatic bacteriuria (asb), occurring in 2-11% of pregnancies, is a major predisposition to the development of pyelonephritis, which is associated with obstetrical complications, such as preterm labor and low birth weight infants. the aim of this study was to determine the prevalence of asb, the antibacterial susceptibilities of the isolated microorganisms and the associated risk factors in an outpatient clinical setting in zekai tahir burak women's health education and researc ...	2011	21647231
synergistic antibacterial effects of polyphenolic compounds from olive mill wastewater.	polyphenols or phenolic compounds are groups of secondary metabolites widely distributed in plants and found in olive mill wastewater (omw). phenolic compounds as well as omw extracts were evaluated in vitro for their antimicrobial activity against gram-positive (streptococcus pyogenes and staphylococcus aureus) and gram-negative bacteria (escherichia coli and klebsiella pneumoniae). most of the tested phenols were not effective against the four bacterial strains when tested as single compounds ...	2011	21647315
the impact of a nationwide antibiotic restriction program on antibiotic usage and resistance against nosocomial pathogens in turkey.	purpose: antimicrobial resistance among microorganisms is a global concern. in 2003, a nationwide antibiotic restriction program (narp) was released in turkey. in this study we evaluated the effect of narp on antibiotic consumption, antimicrobial resistance, and cost.materials and methods: the data obtained from all of the four university hospitals, and one referral tertiary-care educational state hospital in ankara. antimicrobial resistance profiles of 14,233 selected microorganisms all grown i ...	2011	21647326