Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| a survey on pulmonary pathogens and their antibiotic susceptibility among cystic fibrosis patients. | this study was performed to investigate frequency and antimicrobial susceptibility of pulmonary pathogens in cystic fibrosis (cf) patients. | 2017 | 22552452 |
| methodological approaches for monitoring opportunistic pathogens in premise plumbing: a review. | opportunistic premise (i.e., building) plumbing pathogens (oppps, e.g., legionella pneumophila, mycobacterium avium complex, pseudomonas aeruginosa, acanthamoeba, and naegleria fowleri) are a significant and growing source of disease. because oppps establish and grow as part of the native drinking water microbiota, they do not correspond to fecal indicators, presenting a major challenge to standard drinking water monitoring practices. further, different oppps present distinct requirements for sa ... | 2017 | 28390237 |
| the inhibition effect of lactobacilli against growth and biofilm formation of pseudomonas aeruginosa. | the emergence of antibiotic-resistant and food-spoilage microorganisms has renewed efforts to identify safe and natural alternative agents of antibiotics such as probiotics. the aim of this study was the isolation of lactobacilli as potential probiotics from local dairy products with broad antibacterial and anti-biofilm activities against antibiotic-resistant strains of pseudomonas aeruginosa and determination of their inhibition mechanism. antibiotic susceptibility and classification of acquire ... | 2017 | 28293865 |
| pily1 promotes legionella pneumophila infection of human lung tissue explants and contributes to bacterial adhesion, host cell invasion, and twitching motility. | legionnaires' disease is an acute fibrinopurulent pneumonia. during infection legionella pneumophila adheres to the alveolar lining and replicates intracellularly within recruited macrophages. here we provide a sequence and domain composition analysis of the l. pneumophila pily1 protein, which has a high homology to pily1 of pseudomonas aeruginosa. pily1 proteins of both pathogens contain a von willebrand factor a (vwfa) and a c-terminal pily domain. using cellular fractionation, we assigned the ... | 2017 | 28326293 |
| annual variations and effects of temperature on legionella spp. and other potential opportunistic pathogens in a bathroom. | opportunistic pathogens (ops) in drinking water, like legionella spp., mycobacteria, pseudomonas aeruginosa, and free-living amobae (fla) are a risk to human health, due to their post-treatment growth in water systems. to assess and manage these risks, it is necessary to understand their variations and environmental conditions for the water routinely used. we sampled premise tap (n cold = 26, n hot = 26) and shower (n shower = 26) waters in a bathroom and compared water temperatures to levels of ... | 2017 | 27815848 |
| mycobacterium abscessus subsp. abscessus is capable of degrading pseudomonas aeruginosa quinolone signals. | pseudomonas aeruginosa employs 2-heptyl-3-hydroxy-4(1h)-quinolone (the pseudomonas quinolone signal, pqs) and 2-heptyl-4(1h)-quinolone (hhq) as quorum sensing signal molecules, which contribute to a sophisticated regulatory network controlling the production of virulence factors and antimicrobials. we demonstrate that mycobacterium abscessus(t) and clinical m. abscessus isolates are capable of degrading these alkylquinolone signals. genome sequences of 50 clinical m. abscessus isolates indicated ... | 2017 | 28303132 |
| the genetic basis for variation in resistance to infection in the drosophila melanogaster genetic reference panel. | individuals vary extensively in the way they respond to disease but the genetic basis of this variation is not fully understood. we found substantial individual variation in resistance and tolerance to the fungal pathogen metarhizium anisopliae ma549 using the drosophila melanogaster genetic reference panel (dgrp). in addition, we found that host defense to ma549 was correlated with defense to the bacterium pseudomonas aeruginosa pa14, and several previously published dgrp phenotypes including o ... | 2017 | 28257468 |
| induction and analysis of bronchus-associated lymphoid tissue. | bronchus-associated lymphoid tissue (balt) forms spontaneously in the lung after pulmonary infection and has been identified as a highly organized lymphoid structure supporting the efficient priming of t cells in the lung. to explore the mechanisms and instructive signals controlling balt neogenesis we used both, a single dose of vaccinia virus mva and repeated inhalations of heat-inactivated pseudomonas aeruginosa (p. aeruginosa). intranasal administration of both pathogens induces highly organ ... | 2017 | 28063045 |
| [cutaneous lesions during hot-tub hypersensitivity pneumonitis: pseudomonas folliculitis ?] | interstitial lung disease, cutaneous rash and elevated serum angiotensin converting enzyme (ace) may suggest diagnoses other than sarcoidosis. | 2017 | 27823913 |
| high-throughput automated microfluidic sample preparation for accurate microbial genomics. | low-cost shotgun dna sequencing is transforming the microbial sciences. sequencing instruments are so effective that sample preparation is now the key limiting factor. here, we introduce a microfluidic sample preparation platform that integrates the key steps in cells to sequence library sample preparation for up to 96 samples and reduces dna input requirements 100-fold while maintaining or improving data quality. the general-purpose microarchitecture we demonstrate supports workflows with arbit ... | 2017 | 28128213 |
| degradation of benzo[a]pyrene by pleurotus ostreatus po-3 in the presence of defined fungal and bacterial co-cultures. | benzo[a]pyrene, a high molecular weight polycyclic aromatic hydrocarbon possesses carcinogenic, teratogenic, and mutagenic properties. the present study focuses on benzo[a]pyrene degradation by pleurotus ostreatus po-3, characterization and identification of metabolites produced and the extent of degradation in the presence of axenic culture of p. ostreatus po-3 and defined co-cultures of the basidiomycete with bacteria and non-basidiomycete fungi. thin-layer chromatography revealed that p. ostr ... | 2017 | 27874973 |
| pneumocystis infection alters the activation state of pulmonary macrophages. | recent studies show a substantial incidence of pneumocystis jirovecii colonization and infection in patients with chronic inflammatory lung conditions. however, little is known about the impact of pneumocystis upon the regulation of pulmonary immunity. we demonstrate here that pneumocystis polarizes macrophages towards an alternatively activated macrophage-like phenotype. genetically engineered mice that lack the ability to signal through il-4 and il-13 were used to show that pneumocystis altern ... | 2017 | 27720434 |
| heme acquisition mechanisms of porphyromonas gingivalis - strategies used in a polymicrobial community in a heme-limited host environment. | porphyromonas gingivalis, a main etiologic agent and key pathogen responsible for initiation and progression of chronic periodontitis requires heme as a source of iron and protoporphyrin ix for its survival and the ability to establish an infection. porphyromonas gingivalis is able to accumulate a defensive cell-surface heme-containing pigment in the form of μ-oxo bisheme. the main sources of heme for p. gingivalis in vivo are hemoproteins present in saliva, gingival crevicular fluid, and erythr ... | 2017 | 26662717 |
| toxicity of herbal extracts used in ethno-veterinary medicine and green-encapsulated zno nanoparticles against aedes aegypti and microbial pathogens. | dengue and chikungunya are arboviral diseases mainly vectored by the mosquito aedes aegypti. presently, there is no treatment for these viral diseases and their prevention is still based on vector control measures. nanopesticides fabricated using herbal extracts as reducing and capping agents currently represent an excellent platform for pest control. in this scenario, the present study assessed the acute toxicity of seven plants employed in ethno-veterinary medicine of southern india, as well a ... | 2017 | 28389893 |
| acidosis increases the susceptibility of respiratory epithelial cells to pseudomonas aeruginosa-induced cytotoxicity. | bacterial infection can lead to acidosis of the local microenvironment. while this is believed to exacerbate disease pathogenesis, the mechanisms by which changes in ph alter disease progression are poorly understood. in this report we test the hypothesis that acidosis enhances respiratory epithelial cell death in response to infection with pseudomonas aeruginosa. our findings support that acidosis in the context of p. aeruginosa infection results in increased epithelial cell cytotoxicity due to ... | 2017 | 28385813 |
| bacteria can proliferate in thawed cryoprecipitate stored at room temperature for longer than 4 h. | although key coagulation factor activities are maintained in thawed cryoprecipitate stored for up to 24 h at ambient temperature, several jurisdictions limit such storage to 4-6 h. here, we separately spiked thawed cryoprecipitate units with four bacterial strains: staphylococcus epidermidis, serratia liquefaciens, pseudomonas putida and pseudomonas aeruginosa. no strains grew in the first 4 h of storage, but by 24 h, three of four exhibited up to 1000-fold proliferation. pathogen inactivation t ... | 2017 | 28382701 |
| search for novel antibacterial compounds and targets. | drug-resistant bacteria including methicillin-resistant staphylococcus aureus (mrsa), multidrug-resistant pseudomonas aeruginosa, and vancomycin-resistant enterococci (vre) have been spreading; however, the development of new antibacterial drugs has not progressed accordingly. novel antibacterial drugs or their candidate seeds need to be developed for effective antibiotic therapy. under these conditions, the search for novel compounds and novel targets is important. in okayama university, as a p ... | 2017 | 28381710 |
| scaffold of selenium nanovectors and honey phytochemicals for inhibition of pseudomonas aeruginosa quorum sensing and biofilm formation. | honey is an excellent source of polyphenolic compounds that are effective in attenuating quorum sensing (qs), a chemical process of cell-to-cell communication system used by the opportunistic pathogen pseudomonas aeruginosa to regulate virulence and biofilm formation. however, lower water solubility and inadequate bioavailability remains major concerns of these therapeutic polyphenols. its therapeutic index can be improved by using nano-carrier systems to target qs signaling potently. in the pre ... | 2017 | 28386534 |
| multidrug resistant pseudomonas mycotic pseudoaneurysm following cardiac transplant bridged by ventricular assistant device. | mycotic pseudoaneurysm of aorta following cardiac surgery is rare but is highly fatal if it is unrecognized and untreated. here, we report a case of a 45-year-old male patient who presented with rapidly progressive multiple pseudoaneurysms of the ascending aorta infected with multidrug resistant (mdr) pseudomonas aeruginosa at 5 weeks after cardiac transplantation, on a background of prior bridging therapy with left ventricular assistant device (lvad). the patient was successfully treated with t ... | 2017 | 28386491 |
| characterization of virulence potential of pseudomonas aeruginosa isolated from bovine meat, fresh fish, and smoked fish. | pseudomonas aeruginosa owns a variability of virulence factors. these factors can increase bacterial pathogenicity and infection severity. despite the importance of knowledge about them, these factors are not more characterized at level of strains derived from local food products. this study aimed to characterize the virulence potential of p. aeruginosa isolated from various animal products. several structural and virulence genes of p. aeruginosa including lasb, exos, algd, plch, pilb, exou, and ... | 2017 | 28386471 |
| low effect of phenanthrene bioaccessibility on its biodegradation in diffusely contaminated soil. | this study focused on the role of bioaccessibility in the phenanthrene (phe) biodegradation in diffusely contaminated soil, by combining chemical and microbiological approaches. first, we determined phe dissipation rates and phe sorption/desorption isotherms for two soils (ppy and pv) presenting similar chronic pah contamination, but different physico-chemical properties. our results revealed that the phe dissipation rate was significantly higher in the pv soil compared to the ppy soil, while ph ... | 2017 | 28390702 |
| zingiber officinale: its antibacterial activity on pseudomonas aeruginosa and mode of action evaluated by flow cytometry. | biofilm formation, low membrane permeability and efflux activity developed by pseudomonas aeruginosa, play an important role in the mechanism of infection and antimicrobial resistance. in the present study we evaluate the antibacterial effect of zingiber officinale against multi-drug resistant strain of p. aeruginosa. the study explores antibacterial efficacy and time-kill study concomitantly the effect of herbal extract on bacterial cell physiology with the use of flow cytometry and inhibition ... | 2017 | 28389345 |
| stigmatellin y - an anti-biofilm compound from bacillus subtilis br4 possibly interferes in pqs-pqsr mediated quorum sensing system in pseudomonas aeruginosa. | hitherto this is the first report pertaining to production of biofilm inhibitory compound(s) (bic) from bacillus subtilis br4 against pseudomonas aeruginosa (atcc 27853) coupled with production optimization. in order to achieve this, combinations of media components were formulated by employing statistical tools such as plackett-burman analysis and central composite rotatable design (ccrd). it was evident that at 35mll(-1) glycerol and 3.8gl(-1) casamino acid, anti-biofilm activity and productio ... | 2017 | 28389154 |
| bacterial isolates and their antimicrobial susceptibility patterns of wound infections among inpatients and outpatients attending the university of gondar referral hospital, northwest ethiopia. | background. the widespread uses of antibiotics, together with the length of time over which they have been available, have led to the emergence of resistant bacterial pathogens contributing to morbidity and mortality. this study was aimed to assess bacterial isolates and their drug susceptibility patterns from inpatients and outpatients with pus and/or wound discharge. methods. a cross-sectional study was conducted at the university of gondar referral hospital from march to may, 2014. wound swab ... | 2017 | 28386280 |
| nitrosomonas europaea biofilm formation is enhanced by pseudomonas aeruginosa. | biofilms are useful in biotechnology applications such as wastewater treatment, where aggregation of cells on surfaces can increase retention of slow-growing organisms such as ammonia-oxidizing bacteria (aob). the formation and morphological development of polymicrobial biofilms including aob are not thoroughly understood. here, we investigated the formation of nitrosomonas europaea aob biofilms in flow cell systems. n. europaea developed substantially greater biovolume in co-culture with hetero ... | 2017 | 28387799 |
| ph-controlled cerium oxide nanoparticle inhibition of both gram-positive and gram-negative bacteria growth. | here, the antibacterial activity of dextran-coated nanoceria was examined against pseudomonas aeruginosa and staphylococcus epidermidis by varying the dose, the time of treatment, and the ph of the solution. findings suggested that dextran-coated nanoceria particles were much more effective at killing p. aeruginosa and s. epidermidis at basic ph values (ph = 9) compared to acidic ph values (ph = 6) due to a smaller size and positive surface charge at ph 9. at ph 9, different particle concentrati ... | 2017 | 28387344 |
| preventing microbial biofilms on catheter tubes using ultrasonic guided waves. | biofilms on indwelling tubes and medical prosthetic devices are among the leading causes of antibiotic-resistant bacterial infections. in this work, a new anti-biofilm catheter prototype was proposed. by combining an endotracheal tube (et) with a group of ultrasonic guided wave (ugw) transducers, the general idea was to prevent bacteria aggregation with ugw vibrations. based on quantitative analysis of ugw propagation, detailed approach was achieved through (a) selection of ultrasonic frequency, ... | 2017 | 28377583 |
| natural isothiocyanates express antimicrobial activity against developing and mature biofilms of pseudomonas aeruginosa. | the antimicrobial properties of natural isothiocyanates (itcs) found in plants such as nasturtium (tropaeolum majus) and horseradish (armoracia rusticana), and the need of new chemotherapeutic options for treatment of infections caused by multidrug-resistant and biofilm-forming gram-negative bacteria such as pseudomonas aeruginosa (pa), led us to evaluate the effects of three major itcs, allylisothiocyanate (aitc), benzylisothiocyanate (bitc), and phenylethyl-isothiocyanate (peitc), and a mixtur ... | 2017 | 28390975 |
| a predictive model for early mortality after surgical treatment of heart valve or prosthesis infective endocarditis. the endoscore. | the aim of this large retrospective study was to provide a logistic risk model along an additive score to predict early mortality after surgical treatment of patients with heart valve or prosthesis infective endocarditis (ie). | 2017 | 28390740 |
| comparison of imipenem and meropenem antibiotics for the maldi-tof ms detection of carbapenemase activity. | a comparison of carbapenem molecules for the detection of carbapenemase-producing bacteria by maldi-tof ms showed that imipenem exhibited higher sensitivity (97%) and specificity (100%) scores for pseudomonas aeruginosa than meropenem. however, meropenem was more efficient (98% sensitivity and 100% specificity) against enterobacteriaceae. | 2017 | 28390706 |
| structural analysis and new drug development against multidrug efflux pumps. | multidrug efflux pumps are important in the multidrug resistance of gram-negative pathogens. however, despite efforts to develop efflux inhibitors, clinically useful inhibitors are not available at present. abi-pp (a pryridopyrimidine derivative) is a mexb-specific inhibitor that does not inhibit mexy; mexb and mexy are principal pumps in pseudomonas aeruginosa. we previously found that drugs were exported through tandem proximal and distal multisite drug-binding pockets. here we describe the fi ... | 2017 | 28381709 |
| polypharmacology approaches against the pseudomonas aeruginosa mvfr regulon and their application in blocking virulence and antibiotic tolerance. | pseudomonas aeruginosa is an important nosocomial pathogen that is frequently recalcitrant to available antibiotics, underlining the urgent need for alternative therapeutic options against this pathogen. targeting virulence functions is a promising alternative strategy as it is expected to generate less selective resistance to treatment compared to antibiotics. capitalizing on our non-ligand based benzamide - benzimidazole (bb) core structure compounds reported to efficiently block the activity ... | 2017 | 28379691 |
| inhibition of pseudomonas aeruginosa biofilm formation by traditional chinese medicinal herb herba patriniae. | new antimicrobial agents are urgently needed to treat infections caused by drug-resistant pathogens and by pathogens capable of persisting in biofilms. the aim of this study was to identify traditional chinese herbs that could inhibit biofilm formation of pseudomonas aeruginosa, an important human pathogen that causes serious and difficult-to-treat infections in humans. a luxcdabe-based reporter system was constructed to monitor the expression of six key biofilm-associated genes in p. aeruginosa ... | 2017 | 28377931 |
| bacteriological profile and antimicrobial susceptibility patterns of bacteria isolated from pus/wound swab samples from children attending a tertiary care hospital in kathmandu, nepal. | in nepal, little is known about the microbiological profile of wound infections in children and their antimicrobial susceptibility patterns. total of 450 pus/wound swab samples collected were cultured using standard microbiological techniques and the colonies grown were identified with the help of biochemical tests. the antimicrobial susceptibility testing was performed by kirby-bauer disc diffusion technique. methicillin-resistant staphylococcus aureus isolates were detected by using cefoxitin ... | 2017 | 28367217 |
| clarithromycin and n-acetylcysteine co-spray-dried powders for pulmonary drug delivery: a focus on drug solubility. | cystic fibrosis (cf) lungs are usually susceptible to pseudomonas aeruginosa colonization and this bacterium is resistant to immune system clearance and drug control. particularly, the biofilm mode of growth protects several microorganisms from host defenses and antibacterial drugs, mainly due to a delayed penetration of the drug through the biofilm matrix. biofilm, together with lung mucus viscosity and tenacity, reduces, therefore, the effectiveness of conventional antibiotic therapy in cf. th ... | 2017 | 28377314 |
| facile green synthesis of baicalein fabricated gold nanoparticles and their antibiofilm activity against pseudomonas aeruginosa pao1. | biofilm formation is one of the major problems associated with chronic diseases and also attributes for the antibiotic resistance in bacteria. in recent times nanoparticles have been utilized to improve the efficacy of the existing antimicrobial and anti-biofilm agents. the ease in functionalization of gold nanoparticles (aunps) makes them a potential carrier for antimicrobial agents. however, the use of physical or chemical methods of the production of nanoparticles is expensive, labour intensi ... | 2017 | 28377235 |
| effects of pseudomonas aeruginosa and streptococcus mitis mixed infection on tlr4-mediated immune response in acute pneumonia mouse model. | our previous research on the diversity of microbiota in the endotracheal tubes (etts) of neonates in the neonatal intensive care unit found that pseudomonas aeruginosa (p. aeruginosa) and streptococcus mitis (s. mitis) were the dominant bacteria on the ett surface and the existence of s. mitis could promote biofilm formation and pathogenicity of p. aeruginosa. toll-like receptor 4 (tlr4), which has been widely detected on the surface of airway epithelial cells, is the important component of the ... | 2017 | 28376744 |
| increased risk of death with recurrent pseudomonas aeruginosa bacteremia. | this study aimed to characterize recurrent pseudomonas aeruginosa blood stream infection (bsi). positive blood cultures for p. aeruginosa were identified over a 3-year period from seven tertiary care hospitals. patients with recurrent bsi were identified. extensive epidemiological, clinical and outcome data were obtained. bsi recurrence was found to be uncommon with 9% of patients having a first relapse of bsi. fourteen percent of these patients went on to have a second relapse of bsi. significa ... | 2017 | 28366610 |
| ceftolozane-tazobactam for the treatment of ventilator-associated infections by colistin-resistant pseudomonas aeruginosa. | the use of colistin for the treatment of multiresistant bacteria has led to the emergence of colistin-resistant strains of gram-negative bacilli. treatment of infections caused by these pan-drug-resistant bacteria is difficult owing to the paucity of effective antibiotics. we report two cases of ventilator-associated respiratory infection caused by pan-drug-resistant, colistin-resistant pseudomonas aeruginosa that were successfully treated with ceftolozane-tazobactam. | 2017 | 28361526 |
| local collection, reaction and analysis with theta pipette emitters. | a mobile nanofluidic device based on theta pipettes was developed for "collect-react-analyze" measurements of small volumes of a sample collected locally from biological samples. specifically, we demonstrate execution of local reactions inside single cells and on pseudomonas aeruginosa biofilms for targeted analysis of metabolites. nanoliter volumes of the sample, post-reaction, were delivered to a mass spectrometer via electrospray ionization (esi) for chemical analysis. a new strategy was deve ... | 2017 | 28361146 |
| bilateral simultaneous infective keratitis. | to analyze the demographics, risk factors, clinical and microbiological characteristics of cases of bilateral simultaneous infective keratitis. | 2017 | 28359825 |
| extended-spectrum beta-lactamase-producing pseudomonas aeruginosa in camel in egypt: potential human hazard. | the rapid increase of extended-spectrum beta-lactamase (esbl) producing bacteria are a potential health hazard. development of antimicrobial resistance in animal pathogens has serious implications for human health, especially when such strains could be transmitted to human. in this study, the antimicrobial resistance due to esbl producing pseudomonas aeruginosa in the camel meat was investigated. | 2017 | 28359312 |
| biofilm formation, antibiotic susceptibility and rapd genotypes in pseudomonas aeruginosa clinical strains isolated from single centre intensive care unit patients. | the aim of this study was to analyse genotypes, antimicrobial susceptibility patterns and serotypes in pseudomonas aeruginosa clinical strains, including the clonal dissemination of particular strains throughout various intensive care units in one medical centre. using random amplified polymorphic dna (rapd-pcr) and p. aeruginosa antisera, 22 different genotypes and 8 serotypes were defined among 103 isolates from 48 patients. no direct association between p. aeruginosa strain genotypes and sero ... | 2017 | 28365878 |
| successful treatment of multidrug-resistant pseudomonas aeruginosa pubic symphysis osteomyelitis with ceftolozane/tazobactam. | new antibiotic options are needed for the treatment of multidrug-resistant (mdr) pseudomonas infections. we present a case of a man aged 64 years with a bladder fistula due to radiation, ultimately causing osteomyelitis of the pubic symphysis. repeated antibiotic courses, without correcting the fistula, resulted in infection with mdr pseudomonas aeruginosa. he was successfully treated for his osteomyelitis through cystectomy, aggressive debridement and a prolonged course of antimicrobials direct ... | 2017 | 28363945 |
| indole-induced activities of β-lactamase and efflux pump confer ampicillin resistance in pseudomonas putida kt2440. | indole, which is widespread in microbial communities, has received attention because of its effects on bacterial physiology. pseudomonas putida and pseudomonas aeruginosa can acquire ampicillin (amp) resistance during growth on indole-amp agar. transcriptome, mutant, and inhibitor studies have suggested that amp resistance induced by indole can be attributed to increased gene expression of ttgab encoding two genes of rnd-type multidrug efflux operons and an ampc encoding β-lactamase. expression, ... | 2017 | 28352264 |
| identification of mupp as a new peptidoglycan recycling factor and antibiotic resistance determinant in pseudomonas aeruginosa. | peptidoglycan (pg) is an essential cross-linked polymer that surrounds most bacterial cells to prevent osmotic rupture of the cytoplasmic membrane. its synthesis relies on penicillin-binding proteins, the targets of beta-lactam antibiotics. many gram-negative bacteria, including the opportunistic pathogen pseudomonas aeruginosa, are resistant to beta-lactams because of a chromosomally encoded beta-lactamase called ampc. in p. aeruginosa, expression of the ampc gene is tightly regulated and its i ... | 2017 | 28351916 |
| an integrated network analysis reveals that nitric oxide reductase prevents metabolic cycling of nitric oxide by pseudomonas aeruginosa. | nitric oxide (no) is a chemical weapon within the arsenal of immune cells, but is also generated endogenously by different bacteria. pseudomonas aeruginosa are pathogens that contain an no-generating nitrite (no2(-)) reductase (nirs), and no has been shown to influence their virulence. interestingly, p. aeruginosa also contain no dioxygenase (fhp) and nitrate (no3(-)) reductases, which together with nirs provide the potential for no to be metabolically cycled (no→no3(-)→no2(-)→no). deeper unders ... | 2017 | 28363762 |
| the use of plasmapheresis in patients with bronchiectasis with pseudomonas aeruginosa infection and inhibitory antibodies. | 2017 | 28362198 | |
| enhancement of antimicrobial activity by liposomal oleic acid-loaded antibiotics for the treatment of multidrug-resistant pseudomonas aeruginosa. | in this study, we examined the efficacy of liposomal oleic acid-based antibiotic formulations on 32 strains of multidrug-resistant pseudomonas aeruginosa (mdrpa). the average size of liposomes were 93.12 ± 2.3 nm holding a negative zeta potential at -57.3 ± 0.89. liposomal antibiotic formulations were tested against 32 mdrpa strains isolated from burn wounds and urine samples, which exhibited an mic of ≤8 μg/ml, whereas mic of free antibiotics ranged from 32 to >1024 μg/ml. the results clearly i ... | 2017 | 28362119 |
| searching for new strategies against biofilm infections: colistin-amp combinations against pseudomonas aeruginosa and staphylococcus aureus single- and double-species biofilms. | antimicrobial research is being pressured to look for more effective therapeutics for the ever-growing antibiotic-resistant infections, and antimicrobial peptides (amp) and antimicrobial combinations are promising solutions. this work evaluates colistin-amp combinations against two major pathogens, pseudomonas aeruginosa and staphylococcus aureus, encompassing non- and resistant strains. colistin (cst) combined with the amp temporin a (temp-a), citropin 1.1 (cit-1.1) and tachyplesin i linear ana ... | 2017 | 28355248 |
| defining the roles of the cation diffusion facilitators in fe(2+)/zn(2+) homeostasis and establishment of their participation in virulence in pseudomonas aeruginosa. | transporters of the cation diffusion facilitator (cdf) family form dimers that export transition metals from the cytosol. the opportunistic pathogen pseudomonas aeruginosa encodes three homologous cdf genes, czcd (pa0397), aitp (pa1297), and yiip (pa3963). the three proteins are required for virulence in a plant host model. disruption of the aitp gene leads to higher fe(2+) and co(2+) sensitivity together with an intracellular accumulation of these ions and to a decreased survival in presence of ... | 2017 | 28373967 |
| lactobacillus paraplantarum 11-1 isolated from rice bran pickles activated innate immunity and improved survival in a silkworm bacterial infection model. | lactic acid bacteria (lab) have high immune system-stimulating activity and are considered beneficial for human health as probiotics in the gut. the innate immune system is highly conserved between mammals and insects. microbe-associated molecular patterns (e.g., peptidoglycan and β-glucan) induce cytokine maturation, which, in silkworm larvae, leads to muscle contraction. the purpose of this study is to find a novel probiotic by using silkworm muscle contraction assay. in the present study, we ... | 2017 | 28373863 |
| effect of aquo-alchoholic extract of glycyrrhiza glabra against pseudomonas aeruginosa in mice lung infection model. | the prevalence of lung infection caused by pseudomonas aeruginosa strains that are classified as multi-drug resistant has increased considerably and is mainly attributed to relative insufficiency of potent chemotherapeutic modalities. the present study was conducted to evaluate the antimicrobial activity of aquo-alcoholic extract of glycyrrhiza glabra against the p. aeruginosa causing lung infection in swiss albino mice. the study involves evaluation of lethal dose of p. aeruginosa in swiss albi ... | 2017 | 28355591 |
| sers detection of the biomarker hydrogen cyanide from pseudomonas aeruginosa cultures isolated from cystic fibrosis patients. | pseudomonas aeruginosa is the primary cause of chronic airway infections in cystic fibrosis (cf) patients. persistent infections are seen from the first p. aeruginosa culture in about 75% of young cf patients, and it is important to discover new ways to detect p. aeruginosa at an earlier stage. the p. aeruginosa biomarker hydrogen cyanide (hcn) contains a triple bond, which is utilized in this study because of the resulting characteristic c≡n peak at 2135 cm(-1) in a raman spectrum. the raman si ... | 2017 | 28349938 |
| the effect of soft tissue infections on device performance in adult cochlear implant recipients. | the aim of this study was to examine the effect of cochlear implant (ci) site infection and its subsequent management on ci mapping and ci performance. risk factors for ci infections and pathogens causing infections were reviewed. treatment options for ci infections were examined. | 2017 | 28353621 |
| the prevalence and resistance patterns of pseudomonas aeruginosa in a tertiary care hospital in kosovo. | pseudomonas aeruginosa is a gram-negative bacterium that continues to a leading cause of opportunistic nosocomial infections. the rapid increase in drug resistance in clinical isolates of this pathogen is a worldwide concern. the aim of this study was to investigate the distribution rate, prevalence and resistance patterns of p. aeruginosa in clinical specimens from the university clinical centre of kosovo (ucck). during a three-year period, 553 p. aeruginosa isolates were collected from patient ... | 2017 | 28353451 |
| in vivo host environment alters pseudomonas aeruginosa susceptibility to aminoglycoside antibiotics. | during host infection, pseudomonas aeruginosa coordinately regulates the expression of numerous genes to adapt to the host environment while counteracting host clearance mechanisms. as infected patients take antibiotics, the invading bacteria encounter antibiotics in the host milieu. p. aeruginosa is highly resistant to antibiotics due to multiple chromosomally encoded resistant determinants. and numerous in vitro studies have demonstrated the regulatory mechanisms of antibiotic resistance relat ... | 2017 | 28352614 |
| once-daily versus multiple-daily dosing with intravenous aminoglycosides for cystic fibrosis. | people with cystic fibrosis, who are chronically colonised with the organism pseudomonas aeruginosa, often require multiple courses of intravenous aminoglycoside antibiotics for the management of pulmonary exacerbations. the properties of aminoglycosides suggest that they could be given in higher doses less often. this is an update of a previously published review. | 2017 | 28349527 |
| pseudomonas aeruginosa alginate overproduction promotes coexistence with staphylococcus aureus in a model of cystic fibrosis respiratory infection. | while complex intra- and interspecies microbial community dynamics are apparent during chronic infections and likely alter patient health outcomes, our understanding of these interactions is currently limited. for example, pseudomonas aeruginosa and staphylococcus aureus are often found to coinfect the lungs of patients with cystic fibrosis (cf), yet these organisms compete under laboratory conditions. recent observations that coinfection correlates with decreased health outcomes necessitate we ... | 2017 | 28325763 |
| evaluation of the pharmacokinetics of imipenem following regional limb perfusion using the saphenous and the cephalic veins in standing horses. | this prospective experimental study goal was to determine the pharmacokinetics of imipenem after intravenous regional limb perfusion (iv-rlp) in standing horses. nine horses participated in the study; that was approved by the university animal care and use committee. one thoracic limb or one pelvic limb of each horse was randomly selected. after the veins were catheterized, an esmarch bandage tourniquet was applied and the catheter was injected with a solution containing 500mg of imipenem. synov ... | 2017 | 28319829 |
| evaluation of antibacterial activity of the bark and leaf extracts of brosimum gaudichaudii trécul against multidrug resistant strains. | brosimum gaudichaudii trécul., a plant that belongs to moraceae family, is found throughout the brazilian cerrado. the antimicrobial activities of ethanolic bark and leaf extracts of b. gaudichaudii were tested against multiresistant bacteria isolated from diabetic foot infections (dfis). antimicrobial activity of the extracts was evaluated by agar disc diffusion (dd) and broth dilution (bd) methods. by bd method, bark (53.85, 45.83%) and leaf (42.31, 50.00%) extracts contained antimicrobial act ... | 2017 | 28326851 |
| molecular β-lactamase characterization of aerobic gram-negative pathogens recovered from patients enrolled in the ceftazidime-avibactam phase 3 trials for complicated intra-abdominal infections: efficacies analyzed against susceptible and resistant subsets. | the correlation of clinical efficacy of ceftazidime-avibactam (plus metronidazole) and meropenem was evaluated in subjects infected with gram-negative isolates having characterized β-lactam resistance mechanisms from the complicated intra-abdominal infection (ciai) phase 3 clinical trials. enterobacteriaceae displaying ceftriaxone and/or ceftazidime mic values of ≥2 μg/ml and pseudomonas aeruginosa with ceftazidime mic values of ≥16 μg/ml were characterized for extended-spectrum β-lactamase (esb ... | 2017 | 28348155 |
| effect of allergic bronchopulmonary aspergillosis on fev1 in children and adolescents with cystic fibrosis: a european cystic fibrosis society patient registry analysis. | to evaluate the effect of allergic bronchopulmonary aspergillosis (abpa) on fev1 percent predicted in children and adolescents with cystic fibrosis. | 2017 | 28325727 |
| left ventricular assist device-related infections: a multicentric study. | implantable left ventricular assist device (lvad) is a major therapeutic progress for end-stage heart failure in selected patients. as their use is expanding, infectious complications are emerging, with limited data available to guide their management. we aimed to better characterize lvad-related infections. | 2017 | 28323195 |
| label-free electrochemiluminescent biosensor for rapid and sensitive detection of pseudomonas aeruginosa using phage as highly specific recognition agent. | a virulent phage named as pap1 was isolated from hospital sewage based on a lambda phage isolation protocol. this phage showed a strong and highly specific binding ability to pseudomonas aeruginosa (p. aeruginosa). using this isolated phage as a recognition agent, a novel electrochemiluminescent (ecl) biosensor was developed for label-free detection of p. aeruginosa. the biosensor was fabricated through depositing phage-conjugated carboxyl graphene onto the surface of a glass carbon electrode. a ... | 2017 | 28334626 |
| noninvasive imaging of stored red blood cell-transfusion aggravating sepsis-induced liver injury associated with increased activation of m1-polarized kupffer cells. | liver injury has a critical effect on the severity and outcome of sepsis. the impact of stored rbcs on the pathogenesis of sepsis-associated hepatic injury is not well understood. therefore, to investigate the effects of stored-rbc transfusion on sepsis-induced liver damage as well as the associated mechanism, we constructed a sepsis mouse model enabling non-invasive imaging of bacterial infection caused by pseudomonas aeruginosa, a common gram-negative respiratory pathogen. we showed that trans ... | 2017 | 28333715 |
| characterization and interstrain transfer of prophage pp3 of pseudomonas aeruginosa. | prophages are major contributors to horizontal gene transfer and drive the evolution and diversification of bacteria. here, we describe the characterization of a prophage element designated pp3 in the clinical pseudomonas aeruginosa isolate pa1. pp3 spontaneously excises from the pa1 genome and circularizes at a very high frequency of 25%. pp3 is likely to be a defective prophage due to its inability to form plaques on p. aeruginosa indicator strains, and no phage particles could be detected in ... | 2017 | 28346467 |
| liposomes loaded with bioactive lipids enhance antibacterial innate immunity irrespective of drug resistance. | phagocytosis is a key mechanism of innate immunity, and promotion of phagosome maturation may represent a therapeutic target to enhance antibacterial host response. phagosome maturation is favored by the timely and coordinated intervention of lipids and may be altered in infections. here we used apoptotic body-like liposomes (abl) to selectively deliver bioactive lipids to innate cells, and then tested their function in models of pathogen-inhibited and host-impaired phagosome maturation. stimula ... | 2017 | 28345623 |
| sphingosine's role in epithelial host defense: a natural antimicrobial and novel therapeutic. | sphingosine is a natural sphingolipid found in membranes of all eukaryotic cells. in addition to its functions in cell signaling, sphingosine has broad-spectrum antimicrobial properties. sphingosine's role as an antimicrobial is important in tissues such as the skin and respiratory epithelium. reduction in the normal sphingosine level is associated with problems related to infection susceptibility. therefore, exogenous sphingosine may be an effective antimicrobial therapeutic. inhaled nebulized ... | 2017 | 28341550 |
| antimicrobial activity of tigecycline and cefoperazone/sulbactam tested against 18,386 gram-negative organisms from europe and the asia-pacific region (2013-2014). | a total of 18,386 organisms, including 13,224 enterobacteriaceae, 3536 pseudomonas aeruginosa, 1254 acinetobacter spp., and 372stenotrophomonas maltophilia were collected from western europe (weu; n=10,021), eastern europe (eeu; n=4957), and the asia-pacific region (apac; n=3408 [1052 from china]) in 2013-2014 as part of the sentry antimicrobial surveillance program and tested by a reference broth microdilution method for susceptibility against tigecycline, cefoperazone/sulbactam, and comparator ... | 2017 | 28341098 |
| ongoing evolution of pseudomonas aeruginosa pao1 sublines complicates studies of dna damage repair and tolerance. | sublines of the major p. aeruginosa reference strain pao1 are derivatives of the original pao1 isolate, which are maintained in laboratories worldwide. these sublines display substantial genomic and phenotypic variation due to ongoing microevolution. here, we examined four sublines, mpao1, pao1-l, pao1-dsm and pao1-ut, originated from different laboratories, and six dna polymerase-deficient mutants from the p. aeruginosa mpao1 transposon library for their employment in elucidation of dna damage ... | 2017 | 28340408 |
| the pseudomonas aeruginosa two-component regulator algr directly activates rsma expression in a phosphorylation independent manner. | pseudomonas aeruginosa is an important pathogen of the immunocompromised, causing both acute and chronic infections. in cystic fibrosis (cf) patients, p. aeruginosa causes chronic disease. the impressive sensory network of p. aeruginosa allows the bacteria to sense and respond to a variety of stimuli found in diverse environments. transcriptional regulators, including alternative sigma factors and response regulators, integrate signals changing gene expression allowing p. aeruginosa to cause inf ... | 2017 | 28320883 |
| structure reveals mechanisms of viral suppressors that intercept a crispr rna-guided surveillance complex. | genetic conflict between viruses and their hosts drives evolution and genetic innovation. prokaryotes evolved crispr-mediated adaptive immune systems for protection from viral infection, and viruses have evolved diverse anti-crispr (acr) proteins that subvert these immune systems. the adaptive immune system in pseudomonas aeruginosa (type i-f) relies on a 350 kda crispr rna (crrna)-guided surveillance complex (csy complex) to bind foreign dna and recruit a trans-acting nuclease for target degrad ... | 2017 | 28340349 |
| alkaline protease contributes to pyocyanin production in pseudomonas aeruginosa. | the role of the alkaline protease (apra) in pyocyanin production in pseudomonas aeruginosa was investigated. apra was overproduced when a plasmid carrying the apra gene was introduced to an apra-deletion mutant strain, eg03; thus, apra-complemented eg03 was used as an overproducing strain. the complemented strain produced higher pyocyanin than the mutant strain in all commercially available media evaluated. particularly, pyocyanin production was higher in the complemented than in the parental st ... | 2017 | 28333255 |
| bronchial microbiome, pa biofilm-forming capacity and exacerbation in severe copd patients colonized by p. aerugirona. | the bronchial microbiome of severe chronic obstructive pulmonary disease patients colonized by pseudomonas aeruginosa was analyzed using 16s rrna gene sequencing to identify differences related to biofilm-forming capacity. | 2017 | 28339291 |
| an early health economic analysis of the potential cost effectiveness of an adherence intervention to improve outcomes for patients with cystic fibrosis. | cystic fibrosis (cf) negatively impacts upon health-related quality of life and survival. adherence to nebulised treatments is low; improving adherence is hypothesised to reduce rates of exacerbation requiring intravenous antibiotics and lung function decline. | 2017 | 28337719 |
| hospital microbial surface colonization revealed during monitoring of klebsiella spp., pseudomonas aeruginosa, and non-tuberculous mycobacteria. | hospital environmental conditions, human occupancy, and the characteristics of the equipment influence the survival of microbial communities and raise a concern with regard to nosocomial infections. the objective of the present work was to use the monitoring of pseudomonas aeruginosa, klebsiella spp. and non-tuberculous mycobacteria as a strategy to improve knowledge on microbial colonization of non-critical equipment and surfaces, in a tertiary hospital from central portugal. a 3-month microbio ... | 2017 | 28337568 |
| antimicrobial susceptibility of 2906 pseudomonasaeruginosa clinical isolates obtained from patients in canadian hospitals over a period of 8 years: results of the canadian ward surveillance study (canward), 2008-2015. | pseudomonas aeruginosa is an important nosocomial pathogen. the purpose of this study was to evaluate the antimicrobial susceptibility profile of p. aeruginosa clinical isolates obtained from inpatients and outpatients at hospitals across canada from january 2008 to december 2015 (canward study). susceptibility testing was performed using broth microdilution, as described by the clinical and laboratory standards institute. in total, 2906 p. aeruginosa isolates were evaluated. the percentage of i ... | 2017 | 28336136 |
| bacterial cell-to-cell signaling promotes the evolution of resistance to parasitic bacteriophages. | the evolution of host-parasite interactions could be affected by intraspecies variation between different host and parasite genotypes. here we studied how bacterial host cell-to-cell signaling affects the interaction with parasites using two bacteria-specific viruses (bacteriophages) and the host bacterium pseudomonas aeruginosa that communicates by secreting and responding to quorum sensing (qs) signal molecules. we found that a qs-signaling proficient strain was able to evolve higher levels of ... | 2017 | 28331600 |
| il-24 promotes pseudomonas aeruginosa keratitis in c57bl/6 mouse corneas. | the aim of this study was to elucidate the expression and functions of il-24 in c57bl/6 mouse corneas in response to pseudomonas aeruginosa infection. among il-20r cytokines, only il-24 was induced at both mrna and protein levels by infection at early time points. the upregulation of il-24 was dampened by flagellin pretreatment, which protects the corneas from microbial infection. time course studies revealed bimodal early and later peaks of il-24 expression, a pattern shared with suppressor of ... | 2017 | 28330899 |
| multicenter evaluation of ceftolozane/tazobactam for serious infections caused by carbapenem-resistant pseudomonas aeruginosa. | a multicenter, retrospective study of patients infected with carbapenem-resistant pseudomonas aeruginosa who were treated with ceftolozane/tazobactam was performed. among 35 patients, pneumonia was the most common indication and treatment was successful in 26 (74%). treatment failure was observed in all cases where isolates demonstrated ceftolozane-tazobactam minimum inhibitory concentrations ≥8 μg/ml. | 2017 | 28329350 |
| activity of amp2041 against human and animal multidrug resistant pseudomonas aeruginosa clinical isolates. | antimicrobial resistance is a growing threat to public health. pseudomonas aeruginosa is a relevant pathogen causing human and animal infections, frequently displaying high levels of resistance to commonly used antimicrobials. the increasing difficulty to develop new effective antibiotics have discouraged investment in this area and only a few new antibiotics are currently under development. an approach to overcome antibiotic resistance could be based on antimicrobial peptides since they offer a ... | 2017 | 28335779 |
| lack of small colony variants of staphylococcus aureus from lower respiratory tract specimens. | small-colony variants (scvs) of staphylococcus aureus are associated with worse lung disease in children with cystic fibrosis (cf), exhibit a higher resistance to antibiotics and co-colonize more commonly with pseudomonas aeruginosa compared to the normal phenotype. the prevalence of scvs in lower airway specimens from children with cf is largely unknown. | 2017 | 28328157 |
| identification of são paulo metallo-beta-lactamase-1-producing pseudomonas aeruginosa in the central-west region of brazil: a case study. | metallo-beta-lactamase production is an important mechanism for carbapenem resistance of pseudomonas aeruginosa , which represents an emerging public health challenge. we report the case of a patient admitted to an intensive care unit, with sepsis caused by multidrug-resistant são paulo metallo-beta-lactamase-1-producing p. aeruginosa . this is the first case of infection by this pathogenic strain in the state of mato grosso do sul, brazil. thus, infection control measures are required for preve ... | 2017 | 28327817 |
| pseudomonas aeruginosa cytochrome c551 denaturation by five systematic urea derivatives that differ in the alkyl chain length. | reversible denaturation of pseudomonas aeruginosa cytochrome c551 (pac551) could be followed using five systematic urea derivatives that differ in the alkyl chain length, i.e. urea, n-methylurea (mu), n-ethylurea (eu), n-propylurea (pu), and n-butylurea (bu). the bu concentration was the lowest required for the pac551 denaturation, those of pu, eu, mu, and urea being gradually higher. furthermore, the accessible surface area difference upon pac551 denaturation caused by bu was found to be the hi ... | 2017 | 28318436 |
| design and synthesis of galactosylated bifurcated ligands with nanomolar affinity for lectin leca from pseudomonas aeruginosa. | lectin leca of pseudomonas aeruginosa is established as a virulent factor. glycoclusters targeting leca able to compete with human glycoconjugates present on epithelial cells are promising candidates to treat p. aeruginosa infection. a family of 32 glycodendrimers of generation 0 and 1 displaying bifurcated bis-galactosides has been designed to interact with leca. the influence of both the central multivalent core and the aglycon of these glycodendrimers on their affinity toward leca has been ev ... | 2017 | 28318079 |
| transcriptomic analyses elucidate adaptive differences of closely-related strains of p. aeruginosa in fuel. | pseudomonas aeruginosa can utilize hydrocarbons, but different strains have varying degrees of adaptation despite their highly conserved genome. p. aeruginosa atcc 33988 is highly adapted to hydrocarbons while strain pao1, a human pathogen, is less-adapted and degrades jet fuel at a slower rate than does atcc 33988. we investigated fuel specific transcriptomic differences between these strains in order to ascertain the underling mechanisms utilized by the adapted strain to proliferate in fuel. d ... | 2017 | 28314727 |
| carbapenem nonsusceptibility with modified oprd in clinical isolates of pseudomonas aeruginosa from india. | this study was undertaken to investigate oprd porin-mediated carbapenem nonsusceptibility in clinical isolates of pseudomonas aeruginosa from a tertiary referral hospital of northeast india. a total of 267 nonduplicate, consecutive clinical isolates of p. aeruginosa were obtained. mutation and expression levels of oprd gene were determined in carbapenem-nonsusceptible carbapenemase-nonproducing isolates. among 19 carbapenem-nonsusceptible carbapenemase-nonproducing isolates, 11 of them demonstra ... | 2017 | 28303836 |
| experimental pseudomonas aeruginosa mediated rhino sinusitis in mink. | the nasal and sinus cavities in children may serve as reservoirs for microorganisms that cause recurrent and chronic lung infections. this study evaluates whether the mink can be used as an animal model for studying pseudomonas aeruginosa mediated rhino-sinusitis since there is no suitable traditional animal model for this disease. | 2017 | 28302328 |
| dissemination of metallo-β-lactamase in pseudomonas aeruginosa isolates in egypt: mutation in blavim-4. | this study was designed to investigate the prevalence of metallo-β-lactamase (mbl) in pseudomonas aeruginosa isolates collected from suez canal university hospital in ismailia, egypt. antibiotic susceptibility testing and phenotypic and genotypic screening for mbls were performed on 147 isolates of p. aeruginosa. mics were determined by agar dilution method for carbapenem that was ≥2 μg/ml for meropenem. mbl genes were detected by multiplex and monoplex pcr for p. aeruginosa-harbored plasmids. m ... | 2017 | 28295668 |
| surface charge modification decreases pseudomonas aeruginosa adherence in vitro and bacterial persistence in an in vivo implant model. | chronic, persistent infections complicate otologic procedures utilizing implantable devices such as cochlear implants or tympanostomy tubes. these infections are thought to be due to the establishment of microbial biofilms on implant surfaces. to address this issue, we hypothesized that surface charge modification may inhibit the formation of pseudomonas aeruginosa biofilms on implant surfaces in vitro and in vivo. | 2017 | 28295372 |
| chlorin e6 mediated photodynamic inactivation for multidrug resistant pseudomonas aeruginosa keratitis in mice in vivo. | following corneal epithelium scratches, mouse corneas were infected with the multidrug resistant (mdr) p. aeruginosa strain pa54. 24 hours later, 0% (for control group), 0.01%, 0.05% or 0.1% chlorin e6 (ce6), a second generation photosensitizer derived from chlorophyll, was combined with red light, for photodynamic inactivation (pdi). 1 hour or 2 days later, entire mouse eyes were enucleated and homogenized for counting colony forming units (cfu) of p. aeruginosa. for comparison, 0.1% ce6 mediat ... | 2017 | 28295043 |
| indicator bacteria community in seawater and coastal sediment: the persian gulf as a case. | the aim of present work was to assess the concentration levels as well as vertical distribution of indicator bacteria including total coliform, fecal coliform, pseudomonas aeruginosa, and heterotrophic plate count (hpc) in the marine environment (seawater and coastal sediments) and evaluate the correlation between indicator bacteria and some physicochemical parameters of surface sediments as well as seawaters. | 2017 | 28293427 |
| pseudomonas aeruginosa infection liberates transmissible, cytotoxic prion amyloids. | patients who recover from pneumonia subsequently have elevated rates of death after hospital discharge as a result of secondary organ damage, the causes of which are unknown. we used the bacterium pseudomonas aeruginosa, a common cause of hospital-acquired pneumonia, as a model for investigating this phenomenon. we show that infection of pulmonary endothelial cells by p. aeruginosa induces production and release of a cytotoxic amyloid molecule with prion characteristics, including resistance to ... | 2017 | 28314768 |
| invitro activity of imipenem-relebactam against gram-negative bacilli isolated from patients with lower respiratory tract infections in the united states in 2015 - results from the smart global surveillance program. | the β-lactamase inhibitor relebactam inactivates class a β-lactamases, including kpc-type carbapenemases, and class c β-lactamases. relebactam combined with imipenem is in clinical development for several indications, including hospital-acquired and ventilator-associated pneumonia. employing clsi-defined broth microdilution methodology, we evaluated the activities of imipenem-relebactam (using imipenem mic breakpoints) and comparators against non-proteeae enterobacteriaceae (n=853) and pseudomon ... | 2017 | 28291628 |
| pseudomonas aeruginosa strain pa01 impairs enzymes of the phosphotransfer network in the gills of rhamdia quelen. | integration of mitochondria with cytosolic atp-consuming/atp-sensing and substrate supply processes is critical for gills bioenergetics, since this tissue plays an important role in the respiratory energy metabolism. the effects of bacterial infection on gills remain poorly understood, limited only to histopathological analyses. thus, the aim of this study was to investigate whether experimental infection by pseudomonas aeruginosa strain pa01 alters the enzymes of the phosphoryltransfer network ... | 2017 | 28284597 |
| antibacterial activity of polihexanide formulations in a co-culture of hacat keratinocytes and staphylococcus aureus and at different ph levels. | complex stalled wounds feature an alkaline milieu that favors tissue destruction and microbial growth. the presence of bacteria in turn perpetuates the inflammatory response. however, only limited knowledge exists of ph dependency on the antibacterial efficacy of phmb or the influence of surfactants or delivery vehicle used in antiseptic formulations. so far, phmb alone has been shown to protect the keratinocytes from bacterial damage in such a co-culture system as well as exhibiting increased a ... | 2017 | 28370821 |
| induction of the pho regulon and polyphosphate synthesis against spermine stress in pseudomonas aeruginosa. | growth of pseudomonas aeruginosa on spermine requires a functional γ-glutamylpolyamine synthetase paua2. not only subjected to growth inhibition by spermine, the paua2 mutant became more sensitive to β-lactam antibiotics in human serum. to explore paua2 as a potential target of drug development, suppressors of the paua2 mutant, which alleviated toxicity, were isolated from selection plates containing spermine. these suppressors share common phenotypic changes including delayed growth rate, retar ... | 2017 | 28370665 |
| efficacy comparison of adjuvants in pcrv vaccine against pseudomonas aeruginosa pneumonia. | vaccination against the type iii secretion system of p. aeruginosa is a potential prophylactic strategy for reducing the incidence and improving the poor prognosis of p. aeruginosa pneumonia. in this study, the efficacies of three different adjuvants, freund's adjuvant (fa), aluminum hydroxide (alum) and cpg oligodeoxynucleotide (odn), were examined from the viewpoint of inducing pcrv-specific immunity against virulent p. aeruginosa. mice that had been immunized intraperitoneally with recombinan ... | 2017 | 28370521 |
| overexpression of ho-1 contributes to sepsis-induced immunosuppression by modulating the th1/th2 balance and regulatory t-cell function. | countervailing anti-inflammatory response and immunosuppression can cause death in late sepsis. depletion and dysfunction of t-cells are critical for developing sepsis-induced immunosuppression. heme oxygenase (ho)-1 has a regulatory effect on differentiation and function of t-cells and anti-inflammatory properties. we therefore investigated the immunosuppressive role of ho-1 in sepsis with a focus on its effects on helper t-cell (th) differentiation and regulatory t-cells (treg). | 2017 | 28368519 |