Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| cytotoxic t cell activity against the 22-kda protein of human respiratory syncytial virus (rsv) is associated with a significant reduction in pulmonary rsv replication. | recombinant vaccinia viruses expressing the rsv f glycoprotein (vac-f), or a previously described chimeric protein consisting of the extracellular domains of the f and g glycoproteins (vac-fg), or the 22-kda membrane protein (vac-22 kda) were evaluated for their ability to protect balb/c mice against infection by rsv subgroup a or subgroup b viruses and for their ability to induce a humoral immune response or a cytolytic t lymphocyte (ctl) response. immunization with vac-f or vac-fg fully protec ... | 1991 | 2024493 |
| group-specific serum antibody responses in children with primary and recurrent respiratory syncytial virus infections. | antigenic group-specific serum antibody responses to first and second respiratory syncytial virus (rsv) infections were studied in children who had been followed longitudinally from early infancy in a research day-care center. plaque-reduction neutralizing (prn) antibody assays and elisas for the fusion (f) and attachment (g) glycoproteins were done using antigens of prototype rsv strains from groups a and b. responses to antigens of viruses homologous and heterologous to the antigenic group of ... | 1991 | 2056202 |
| evaluation of an enzyme membrane immunoassay (directigen rsv) for the rapid diagnosis of respiratory syncytial virus infections. | a rapid enzyme immunoassay (eia) membrane test, the directigen respiratory syncytial virus (becton dickinson), was compared with cell culture, an indirect immunofluorescence (if) test, the monofluokit respiratory syncytial virus (diagnostics pasteur), and a conventional enzyme immunoassay antigen test, the abbott respiratory syncytial virus enzyme immunoassay in nasal aspirates specimens from children with suspected respiratory syncytial virus (rsv) bronchiolitis. the sensibility and specificity ... | 1991 | 2062553 |
| dual-enzyme cascade-magnetic separation immunoassay for respiratory syncytial virus. | a new immunoassay developed for the detection of respiratory syncytial virus (rsv) makes use of magnetic separation and amplification by a dual-enzyme cascade for signal generation. magnetic particles are conjugated to monoclonal anti-rsv antibodies through the heterobifunctional crosslinker sulfosuccinimidyl 4-(maleimidomethyl)cyclohexane-1-carboxylate, yielding particles of high specific activity and low background. the dual-enzyme cascade is initiated by activation of a masked inhibitor for t ... | 1991 | 2010616 |
| early use of indirect immunofluorescence for the detection of respiratory syncytial virus in hep-2 cell culture. | respiratory syncytial virus is detected in cell culture by the presence of cytopathic effect. to detect rsv before cytopathic effect is usually seen, slides were evaluated retrospectively from 482 hep-2 cell cultures on days 2-4 after inoculation. indirect immunofluorescent staining detected rsv in 57 of 94 cultures that eventually were found positive by cytopathic effect. in an additional 19 cases that ultimately showed no cytopathic effect, rsv also was detected. in 15 of the latter cases, the ... | 1991 | 2069130 |
| respiratory syncytial virus infection in c57bl/6 mice: clearance of virus from the lungs with virus-specific cytotoxic t cells. | we describe respiratory syncytial virus (rsv)-specific cytotoxic t-cell (ctl) lines and clones developed from the spleens of c57bl/6 and balb/c mice. line 7 and clones derived from it were h-2kb restricted, whereas line 12 had both kb and db components. both lines, and all the clones except one, could lyse targets infected with either strain a or strain b rsv. line 7 or 7-11e1 cells (8 x 10(6) to 10 x 10(6) given intravenously cleared rsv from the lungs of infected mice. there was no morbidity o ... | 1991 | 2072460 |
| pattern of respiratory syncytial virus epidemics in finland: two-year cycles with alternating prevalence of groups a and b. | time-resolved fluoroimmunoassay with monoclonal antibodies distinguishing between respiratory syncytial virus (rsv) group a and b strains was used to analyze their prevalence in finland during 1981-1990 among 3285 patients with laboratory diagnosis of rsv, most of them hospitalized. the group typing of antigens in 608 rsv-positive nasopharyngeal aspirates showed a regular alternation of group prevalence, following the cyclic occurrence of the virus. group a predominated in 73%-90% of specimens f ... | 1991 | 1995719 |
| detection of respiratory syncytial virus in nasopharyngeal secretions by shell vial technique. | a shell vial technique was used to recover respiratory syncytial virus (rsv) from frozen nasopharyngeal specimens previously tested by rapid diagnostic methods. with specimens determined to be positive by direct fluorescence assay (dfa), the shell vial technique was at least as sensitive as conventional tissue culture (92 versus 90%). the majority of rsv isolates were detected within 16 h postinoculation, versus an average of 4.5 days by conventional techniques. also, the shell vial method recov ... | 1991 | 2037662 |
| invasion by wc5 rat cerebellar cells is independent of rsv-induced changes in growth and adhesion. | the wc5 rat cerebellar cell line, which is infected with a rous sarcoma virus that is temperature-sensitive for pp60src transformation, shows temperature-dependent expression of the neural-cell-adhesion molecule (n-cam) and glial fibrillary acidic protein (gfap). we found that wc5 cells maintained at the non-permissive temperature in both monolayer cultures and spheroids are subject to density-dependent inhibition of growth, whereas cells maintained at the permissive temperature continued to gro ... | 1991 | 1652569 |
| complex expression pattern of the cef-4 cytokine in transformed and mitogenically stimulated cells. | the transformation of chicken embryo fibroblasts (cef) by the rous sarcoma virus leads to the constitutive expression of the cellular gene designated cef-4. with specific antisera, we confirmed that transformed cells actively synthesize and secrete a 6 kda polypeptide corresponding to the cef-4 gene product. the expression of cef-4 was investigated by northern and immunoprecipitation analyses. upon activation of pp60v-src in cells infected by a ts mutant of rsv, the expression of cef-4 was bipha ... | 1991 | 1656364 |
| characterization of rous sarcoma virus intronic sequences that negatively regulate splicing. | retroviruses splice only a fraction of their primary rna transcripts to subgenomic mrna. the unspliced rna is transported to the cytoplasm, where it serves as genomic rna as well as mrna for the gag and pol genes. deletion of sequences from the rous sarcoma virus gag gene, which is part of the intron of the subgenomic mrnas, was previously observed to result in an increase in the ratio of spliced to unspliced rna. these sequences, which we termed a negative regulator of splicing (nrs), can be mo ... | 1991 | 1656608 |
| detection of rotavirus antigen in tracheal aspirates of infants and children with pneumonia. | clinical manifestations of respiratory tract infection often precede or coincide with rotavirus gastroenteritis in infants and children. to investigate the possible association between respiratory tract manifestations and rotavirus infection, the authors determined human rotavirus (hrv) antigen and respiratory syncytial virus (rsv) antigen in tracheal aspirates of 58 children with clinically diagnosed pneumonia by enzyme-linked immunosorbent assay (elisa) and immunofluorescent antibody technique ... | 1991 | 1661224 |
| recombinant reverse transcriptase of rous sarcoma virus: characterization of dna polymerase and rnaase h activities. | enzyme preparations of rous sarcoma virus (rsv) reverse transcriptase have been isolated from a culture of e. coli hb101(pmf14). the enzyme has been purified to homogeneity and been shown to consist of two subunits, of molecular mass 97.4 and 61.3 kda, respectively. the optimum conditions for the dna polymerase and rnaase h activities, fidelity of dna synthesis on a homogeneous rna template, and the inhibitory effect of azidothymidine triphosphate have been determined. data on the use of rsv rec ... | 1991 | 1717011 |
| production of a transforming growth factor-beta-like growth factor by rsv-transformed rat cerebral microvascular endothelial cells. | this study describes the preliminary characterization of a rous sarcoma virus (rsv)-transformed rat cerebral microvascular endothelial cell (rce-t1)-derived growth factor with biological properties similar to transforming growth factor-beta (tgf-beta). a significant portion of this rce-t1-derived tgf-beta-like growth factor was secreted in an active form and its secretion was elevated 2- to 3-fold in late passage cultures comprised of rapidly growing, less differentiated cells exhibiting reduced ... | 1991 | 1648783 |
| assembly and processing of avian retroviral gag polyproteins containing linked protease dimers. | assembly and maturation of retroviral particles requires the aggregation and controlled proteolytic cleavage of polyprotein core precursors by a precursor-encoded protease (pr). active, mature retroviral pr is a dimer, and the accumulation of precursors at sites of assembly may facilitate subunit interaction and subsequent activation of this enzyme. in addition, it has been suggested that cellular cytoplasmic components act as inhibitors of pr activity, so that processing is delayed until the na ... | 1991 | 1656090 |
| [virological surveillance of acute respiratory tract illnesses of children in morioka, japan. i. epidemiological patterns of infection with respiratory viruses over a 10-year period]. | a virological surveillance of acute respiratory tract illnesses (rti) of children in morioka, japan, was maintained from september 1973 to december 1983. nasal and throat swabs were collected from 4,334 children with rti. these patients consisted of 3,500 children (80.8%) with upper rti (urti) and 834 children (16.2%) with lower rti. when these patients with urti were classified by maximum temperature recorded into 4 groups (less than or equal to 37.0 degrees c, 37.1-37.9 degrees c, 38.0-38.4 de ... | 1991 | 1649233 |
| a new variant of rous sarcoma virus-33 nondefective, pathogenic for rats. | rous sarcoma virus-33 (rsv-33) belongs to rsvs that have the least number of passages beyond its isolation from chicken tumor no. 1 among all current strains of rsv. biological characterization indicated that it was pathogenic for rats. the results of the proviral restriction enzyme analysis showed that the established rat tumorigenic cell lines were the most likely infected by the same virus having a full-length genome. | 1991 | 1662779 |
| transcription of muscle-specific genes is repressed by reactivation of pp60v-src in postmitotic quail myotubes. | quail myogenic cells infected with temperature sensitive (ts) mutants of rous sarcoma virus (rsv) exhibit a temperature-dependent transformation and block of differentiation. when the cells are allowed to differentiate at the restrictive temperature (41 degrees c) and then shifted back to the permissive temperature (35 degrees c), a sharp reduction in the accumulation of muscle-specific mrnas is observed, following reactivation of the transforming protein pp60v-src. a kinetic analysis of this do ... | 1991 | 1645448 |
| rapid diagnosis of viral respiratory infections. comparison between immunofluorescence on clinical samples and immunofluorescence on centrifuged cell cultures. | we compared the technique of immunofluorescence on clinical samples (ifcs) and immunofluorescence on centrifuged cell cultures (ifcc) for the detection of respiratory viruses, using monoclonal antibodies. four hundred fourty five nasopharyngeal aspirates were tested prospectively for rsv, influenza- (a and b), parainfluenza viruses (1, 2, 3) and adenovirus. ifcs and ifcc detected 94% and 51% of rsv respectively, 78.5% and 82% of influenza viruses, 8.3% and 100% of parainfluenzaviruses and 0 and ... | 1991 | 1645920 |
| pexpress: a family of expression vectors containing a single transcription unit active in prokaryotes, eukaryotes and in vitro. | we have constructed a family of expression vectors containing a single transcription unit that is active in escherichia coli, eukaryotic cells, and in coupled in vitro transcription-translation systems. these vectors use the rous sarcoma virus-long terminal repeat (rsv-ltr) as the promoter/enhancer for eukaryotic cells. in vitro transcription is made possible by inclusion of a bacteriophage t7 promoter. this same promoter is actively transcribed in e. coli that produce t7 rna polymerase. other f ... | 1991 | 1657716 |
| the isopycnic, compartmentalized integration of rous sarcoma virus sequences. | rous sarcoma virus (rsv) can cause tumors in hamsters, which harbor complete or partially deleted rsv sequences, in their genomes. here we have studied the localization of rsv sequences integrated into the genome of cell lines derived from six independent hamster tumors. we have found that integration occurred in the isochores richest in guanine + cytosine, of the host genome, as it had been previously observed for bovine leukemia and hepatitis b viral sequences. the integration of rsv proviral ... | 1991 | 1657723 |
| importance of sv40 early 5' distal sequences in directing heterologous gene expression. | the chloramphenicol acetyltransferase-encoding reporter gene (cat) is used extensively in assessing the ability of transcriptional regulatory elements (tre) to direct gene expression in eukaryotic cells. two commonly utilized plasmids contain the cat coding sequences under the transcriptional control of the rous sarcoma virus ltr (prsvcat) or simian virus 40 early (sv40e) promoter (psv2cat). in the present study, we have recloned the rsv-ltr and sv40e tre into a puc18 vector. direct comparison o ... | 1991 | 1657724 |
| v-src genes in two cell lines of so-called rsv-transformed human cells are defective and inactive. | trial of rous sarcoma virus (rsv) induction by cell fusion with chick embryo cells (cec) and wing web test from so-called rsv-transformed human cells, kc and rsb cells, was unsuccessful. the loss of rsv inducibility was also confirmed by dna transfection method. southern blot and northern blot hybridization of dna and rna from those cells with the v-src probe revealed that the v-src genes in those cells were defective and not expressed. on the other hand, the v-src gene in rsv-transformed mouse ... | 1991 | 1651045 |
| pathogenesis of severe acute respiratory infections in the developing world: respiratory syncytial virus and parainfluenza viruses. | respiratory syncytial virus (rsv) and parainfluenza viruses (pivs) are the most frequently isolated pathogens in infants and children with acute lower respiratory infection (lri) in the developed world. less information is available about their importance in lri in the developing world, but they are probably important there also. the pathogenesis of viral bronchiolitis and pneumonia involves inoculation and early replication in the upper respiratory tract, followed by aspiration into the lower r ... | 1991 | 1650494 |
| transformation-dependent activation of urokinase-type plasminogen activator by a plasmin-independent mechanism: involvement of cell surface membranes. | transformed cells produce elevated levels of the urokinase-type plasminogen activator (u-pa), which has been linked with the invasive or migratory phenotype of these cells. the u-pa is secreted and normally maintained in the inactive, single-chain form (scu-pa) and it has been assumed that natural activation occurs via a plasmin-mediated cleavage converting scu-pa to the active, two-chain form (tcu-pa). we now demonstrate that secreted scu-pa in rous sarcoma virus-transformed chicken embryo fibr ... | 1991 | 1652763 |
| isolation of adenovirus from lambs with upper respiratory syndrome. | the role of viruses in the etiology of recurrent upper respiratory disease in newly weaned lambs was studied during 1984-1985 at the north dakota sheep experiment station. serum samples collected from lambs at weaning, from lambs with signs of respiratory disease, and 3 weeks following the onset of clinical signs were tested for antibodies to ovine adenovirus (oav), respiratory syncytial virus (rsv), and parainfluenza type-3 virus (pi-3). virus isolation studies were performed on nasal secretion ... | 1991 | 1655060 |
| [induction of unstable mutations in drosophila melanogaster by microinjections of oncogenic virus dna into the embryo polar plasma. prolonged genetic instability of mutations at the lobe locus]. | mutations lobe induced by the microinjections of rsv cdna into drosophila melanogaster early embryos are characterized by permanent genetic instability; the level of this instability is being changed in time. based on the results of genetic analyses of lobe mutations and molecular analysis of white and adh mutations induced at high frequency in this system of gene instability, we supposed that unstable mutations which arose under the influence of retroviral cdna are of the insertional nature. | 1991 | 1652537 |
| modulation of intercellular adherens-type junctions and tyrosine phosphorylation of their components in rsv-transformed cultured chick lens cells. | transformation of cultured chick lens epithelial cells with a temperature-sensitive mutant of rous sarcoma virus (tsrsv) leads to radical changes in cell shape and interactions. when cultured at the restrictive temperature (42 degrees c), the transformed cells largely retained epithelial morphology and intercellular adherens junctions (aj), whereas on switch to the permissive temperature (37 degrees c) they rapidly became fibroblastoid, their aj deteriorated, and cell adhesion molecules (a-cam) ... | 1991 | 1650581 |
| dimerization of human immunodeficiency virus (type 1) rna: stimulation by cations and possible mechanism. | the retroviral genome consists of two identical rna molecules joined close to their 5' ends by the dimer linkage structure. recent findings indicated that retroviral rna dimerization and encapsidation are probably related events during virion assembly. we studied the cation-induced dimerization of hiv-1 rna and results indicate that all in vitro generated hiv-1 rnas containing a 100 nucleotide domain downstream from the 5' splice site are able to dimerize. rna dimerization depends on the concent ... | 1991 | 1645868 |
| reshaping a human monoclonal antibody to inhibit human respiratory syncytial virus infection in vivo. | we transferred the complementarity determining regions from a murine monoclonal antibody that neutralizes infection by respiratory syncytial virus (rsv) to a human igg1 monoclonal antibody. the resulting reshaped human antibody lost affinity for rsv, but an additional alteration to one of the framework regions restored binding affinity and specificity. this second generation reshaped human monoclonal antibody cross-reacted with all clinical isolates of rsv tested and both prevented disease and c ... | 1991 | 1367535 |
| what every laboratorian should know about rsv (respiratory syncytial virus). | 1991 | 10108905 | |
| a group level analysis of the associations between antibodies to seven putative pathogens and respiratory disease and weight gain in ontario feedlot calves. | the associations, at the group level, between serological titer to pasteurella haemolytica surface antigens (ph), pasteurella haemolytica cytotoxin (ph-cytox), infectious bovine rhinotracheitis virus (ibrv), bovine virus diarrhea virus (bvdv), parainfluenza-3 virus (piv3), respiratory syncytial virus (rsv), mycoplasma dispar (md), m. bovis (mb), and respiratory disease treatment rates, relapse rates, and 28 day weight gains were investigated in 14 groups of calves entering two feedlots during ye ... | 1990 | 2165846 |
| targeted immunoglobulin therapy for the prevention of neonatal infections. | premature infants demonstrate hypoglobulinemia and are at increased risk for serious infections. although a cause-and-effect relation between low serum igg levels and neonatal infections has not been established, prophylaxis of such severe infections may be possible by replacement of antibody with intravenous immunoglobulin (ivig). for success, ivig must provide specific antibodies to neonatal pathogens and reach therapeutic serum igg target levels. pilot investigations have demonstrated that iv ... | 1990 | 2114035 |
| a rapid test for detection of respiratory syncytial virus in nasopharyngeal secretion. | a new rapid membrane enzyme immunoassay (meia; directigen rsv) for detection of respiratory syncytial virus (rsv) was evaluated using samples of nasopharyngeal secretion from infants and children with acute respiratory disease. the meia was compared with an immunofluorescent antibody (if) technique using a sensitive biotin-avidin (ba) eia as reference. of 242 samples tested, 108 were positive by the meia and 123 by the ba-eia. of 144 samples which were also tested by the if technique, 57 were po ... | 1990 | 2197094 |
| occurrence of groups a and b of respiratory syncytial virus over 15 years: associated epidemiologic and clinical characteristics in hospitalized and ambulatory children. | over 15 years respiratory syncytial virus (rsv) isolates from 1209 hospitalized and ambulatory children were examined for strain group and in a subset for subgroup to determine the associated epidemiologic and clinical characteristics. three patterns of yearly outbreaks existed: (1) strong predominance of group a strains (9 years with 83%-100% a strains), (2) relatively equal proportions of group a and b strains (4 years), and (3) strong predominance of group b strains (78%-85%) in 2 years, sepa ... | 1990 | 2230258 |
| refractory congestive heart failure after ribavirin in infants with heart disease and respiratory syncytial virus. | although treatment with ribavirin has been known to be associated with a decreased mortality in infants with congenital heart disease (chd) who have respiratory syncytial virus (rsv), few data are available regarding morbidity. we reviewed records of 10 consecutively hospitalized infants with chd during a recent rsv epidemic. despite the presence of left-to-right shunt in each patient, symptoms of rsv were respiratory at presentation. after ribavirin, decreased respiratory symptoms were found in ... | 1990 | 2308658 |
| severe respiratory syncytial virus pneumonia in an adult renal transplant recipient: successful treatment with ribavirin. | respiratory syncytial virus (rsv) pneumonia may be a severe disease especially in immunocompromised patients. it is quite rare in adults. we report a case of severe rsv pneumonia in a 38-year-old male renal transplant recipient with successful outcome after ribavirin aerosol therapy. | 1990 | 2320966 |
| igg-subclass-specific antibody reactivity to respiratory syncytial virus polypeptides investigated by western blot. | igg1 and igg3 subclass-specific antibody reactivity (abr) in serum samples obtained from infants and children in relation to acute lower respiratory disease caused by respiratory syncytial virus (rsv) infection was investigated by western blot. igg1 abr was directed against the nucleocapsid polypeptides vpn and vpp as well as against the glycoproteins gp48 (f1) and gp90. igg3 abr was directed only against vpn and vpp. in infants, a low igg1 reactivity against glycoproteins was observed. when ser ... | 1990 | 2392617 |
| isolation of respiratory syncytial virus from liver tissue and extrahepatic biliary atresia material. | respiratory syncytial virus (rsv) was isolated from liver tissue in a 7-month-old infant with extrahepatic biliary atresia. to our knowledge this is the first report of rsv isolation from human liver tissue. | 1990 | 2320967 |
| chest x-ray appearances in pneumonia and bronchiolitis. correlation to virological diagnosis and secretory bacterial findings. | chest x-ray findings were related to virus diagnosis, age and secretory bacterial findings in 128 infants and children under 7 years of age with clinical pneumonia and bronchiolitis. they belonged to a cohort examined in connection with the introduction of rapid methods for virological diagnosis. seventy-six children had a virus infection diagnosed by examination of nasopharyngeal secretion and/or by serological methods. thirty-seven of these children were classified as having pathogenic bacteri ... | 1990 | 2321485 |
| a study of nonbacterial agents of acute lower respiratory tract infection in thai children. | from january 1986 to december 1987, 596 children less than 5 years of age with lower respiratory tract infection (lri)--manifested as laryngitis, croup, bronchitis, bronchiolitis, and pneumonia--were studied for evidence of infection with respiratory tract viruses mycoplasma pneumoniae, and chlamydia trachomatis. of the 596 children in the study, 315 were ambulatory and 281 were hospitalized. virologic studies included isolation and rapid diagnosis of virus from specimens of nasopharyngeal aspir ... | 1990 | 2125359 |
| respiratory syncytial virus modulation of adult and neonatal lymphocyte mitogenic responses and the role of interferon-gamma. | infection of human adult and neonatal mononuclear leukocytes (mnls) with respiratory syncytial virus (rsv) caused significant inhibition of lymphoproliferation in response to various mitogens (heat-killed staphylococcus aureus, phytohaemagglutinin, concanavalin-a and pokeweed mitogen). antibody to human interferon-gamma enhanced the rsv-induced suppression of lymphoproliferation in adult, but not neonatal mnls. these results are consistent with the hypothesis that rsv can suppress the developmen ... | 1990 | 2129048 |
| lymphocyte subpopulations in peripheral blood of lambs experimentally infected with bovine respiratory syncytial virus. | the lymphocyte subpopulations of peripheral blood of normal lambs and lambs experimentally infected with bovine respiratory syncytial virus (rsv) were analysed by flow cytometry, using a panel of monoclonal antibodies against specific lymphocyte epitopes. experimental infection with bovine rsv was characterized by a significant rise in sbu-t8+ (cd8+ or cytotoxic) t cells and a significant reduction in sbu-t4+ (cd4+ or helper) t cells and b (lca p220+) lymphocytes (p less than 0.05). the helper/s ... | 1990 | 2140217 |
| detection of respiratory syncytial virus antigen after seventy-two hours of culture. | between september, 1987, and april, 1989, three techniques for the detection of respiratory syncytial virus (rsv) were compared: indirect immunofluorescence (if) on the sample, indirect immunofluorescence after 72 h of mrc-5 cell culture (if 72h), and detection of the cytopathic effect (cpe) by mrc-5 and hep-2 cell culture. a study of 383 nasal aspirates from young children admitted to the centre hospitalier de nantes (chr) showing miscellaneous respiratory symptoms produced the following result ... | 1990 | 2202784 |
| virus-induced alterations in macrophage production of tumor necrosis factor and prostaglandin e2. | the cellular immune response to respiratory syncytial virus (rsv) is felt to contribute to viral clearance and/or the inflammation accompanying pulmonary infections with this virus. both tumor necrosis factor (tnf) and prostaglandin e2 (pge2) are important regulatory mediators of the cellular immune response. we examined the production of these mediators from purified human alveolar and blood mononuclear phagocytes (mp) after rsv infection in vitro and compared production induced by virus with t ... | 1990 | 2260674 |
| comparison of immunofluorescence & culture for the diagnosis of respiratory syncytial virus infection. | in 809 infants and children with acute respiratory infection, hep-2 cells were used for the isolation of respiratory syncytial virus (rsv) and an indirect immunofluorescence technique (iif) was used for the detection of rsv antigen in the epithelial cells of nasopharyngeal secretions. while rsv was detected in culture in only 87 subjects, iif was positive for viral antigen in 158 subjects. in children with bronchiolitis and in those with pneumonia 57 and 19 per cent respectively, had evidence of ... | 1990 | 2228050 |
| rapid recovery in mice after combined nasal/oral immunization with killed respiratory syncytial virus. | based on the concept of a common mucosal immune system, the murine gastrointestinal tract was inoculated (oral) with three doses (5, 20, and 40 micrograms) of uv-inactivated respiratory syncytial virus (rsv) in order to elicit a virus-specific immune response in the respiratory tract. only the 40 micrograms dose induced significant (p less than 0.01) anti-rsv-igg rises in serum and lung wash compared to controls. to improve the immune response, mice were immunized intranasally under light anesth ... | 1990 | 2243208 |
| the two open reading frames of the 22k mrna of human respiratory syncytial virus: sequence comparison of antigenic subgroups a and b and expression in vitro. | the sequence of the 22k mrna of strain 18537 of antigenic subgroup b of human respiratory syncytial virus (rsv) was determined by sequencing cloned cdnas of intracellular mrna. comparison with the corresponding sequence of the a2 strain of subgroup a showed that there is 78% nucleotide sequence identity overall, that the amino acid sequence of the 22k protein is 92% identical between subgroups and that the 22k mrna of both subgroups contains a second, internal, overlapping open reading frame (or ... | 1990 | 2273393 |
| severe respiratory syncytial virus infection in older children. | serious respiratory syncytial virus (rsv) disease requiring hospitalization occurs primarily in infants younger than 12 months. the incidence, risk factors, and clinical features in older children have not been studied extensively. of 282 children hospitalized at our institution with severe rsv disease during a 3-year period, 62 (22%) were older than 12 months. these 62 older children were matched for sex, onset of illness, and hospital location with 62 hospitalized children younger than 12 mont ... | 1990 | 2305743 |
| evaluation of direct immunofluorescence, enzyme immunoassay, centrifugation culture, and conventional culture for the detection of respiratory syncytial virus. | four methods of detecting respiratory syncytial virus (rsv) from clinical specimens were evaluated. a total of 410 specimens consisting of nasopharyngeal washes, aspirates, and swabs were simultaneously tested for the presence of rsv by direct immunofluorescence assay (dfa), enzyme immunoassay (eia) (kallestad pathfinder), shell vial centrifugation culture (svc), and conventional culture. dfa identified 146 (83%) of the 175 positive cases, eia detected 153 (87%), svc detected 127 (73%), and conv ... | 1990 | 2254415 |
| evaluation in chimpanzees of vaccinia virus recombinants that express the surface glycoproteins of human respiratory syncytial virus. | the immunogenicity and protective efficacy of recombinant vaccinia viruses that express the two major protective antigens of human respiratory syncytial virus (rsv), the f and g glycoproteins, were evaluated in chimpanzees. in previous studies in rodents and monkeys the f and g proteins expressed by the same recombinants were highly immunogenic and induced high levels of resistance to rsv replication following subsequent challenge. in contrast, in chimpanzees, a single intradermal immunization i ... | 1990 | 2336876 |
| screening for respiratory syncytial virus and assignment to a cohort at admission to reduce nosocomial transmission. | to limit nosocomial spread of respiratory syncytial virus (rsv) infection, a longitudinal intervention trial was instituted. nasal secretions or washes were screened for rsv antigen by enzyme-linked immunosorbent assay, and patients were assigned to an rsv-infected or an rsv-uninfected cohort. the baseline (preintervention) rate of 7.17 nosocomial cases of rsv per 1000 patient-days of care was used for comparison. despite continued infections in the community after screening was initiated, there ... | 1990 | 2348292 |
| [a bronchiolitis epidemic caused by respiratory syncytial viruses]. | in the typical period of the year (autumn-winter 1986-1987) an outbreak of bronchiolitis in infants caused by respiratory syncytial virus (rsv) occurred. the acute course of the illness, a severe clinical picture, and a very fast spread of infection called for the very rapid techniques for detecting the etiological agent. applying the direct immunofluorescent antibody test (dfta) in samples of nasopharyngeal secretions, rsv was found to be responsible for the epidemic. later the same samples of ... | 1990 | 2396494 |
| an outbreak of an influenza-like illness in a nursing home. | as part of an ongoing infection surveillance program, data were collected on an outbreak of respiratory illness affecting 29% of residents in a 231-bed long-term care facility. viral titers could be collected on 60% of the ill residents and respiratory syncytial virus (rsv) was established in 14 of the symptomatic patients and was thought to be the probable cause of the outbreak. compared with previously reported long-term institutional outbreaks of rsv, there were significantly fewer cases of p ... | 1990 | 2358628 |
| identification of viruses in a study of acute respiratory tract infection in children from uruguay. | for the purpose of identifying viral agents associated with acute respiratory tract infections (ari) in children less than 5 years old, a longitudinal community study was undertaken in montevideo, uruguay, from may 1985 to december 1987. this report includes results obtained by cell culture and immunofluorescence techniques for detection of respiratory syncytial virus (rsv), influenza a and b viruses, parainfluenza 1 and 3 viruses, and adenovirus. two populations were studied: children visited a ... | 1990 | 2270420 |
| association between respiratory syncytial virus outbreaks and lower respiratory tract deaths of infants and young children. | the temporal patterns of respiratory virus isolations from 10 laboratories in the usa were compared with that of deaths of children less than 5 years old from july 1975 through june 1984. isolations of respiratory syncytial virus (rsv) occurred as yearly winter outbreaks; parainfluenza virus 1 and 2 isolations occurred as well-defined outbreaks every other year in the autumn; parainfluenza virus 3 isolations occurred throughout the year with periodic, increased isolations suggestive of outbreaks ... | 1990 | 2319164 |
| productive infection of isolated human alveolar macrophages by respiratory syncytial virus. | respiratory syncytial virus (rsv) is a significant cause of lower respiratory tract disease in children and individuals with cell-mediated immunodeficiencies. airway epithelial cells may be infected with rsv, but it is unknown whether other cells within the lung permit viral replication. we studied whether human alveolar macrophages supported rsv replication in vitro. alveolar macrophages exposed to rsv demonstrated expression of rsv fusion gene, which increased in a time-dependent manner and co ... | 1990 | 2365811 |
| ambisense segment 4 of rice stripe virus: possible evolutionary relationship with phleboviruses and uukuviruses (bunyaviridae). | the complete nucleotide sequence of segment 4 of the rice stripe virus (rsv) genome was determined from overlapping cdna clones and by direct rna sequencing. the segment has two long open reading frames (orfs). one of the orfs (534 bases) is in the 5' region of the viral sequence, and the other (858 bases) is in the viral complementary sequence of the viral 3' region. such ambisense genome organizations have been seen previously in rnas of phleboviruses, uukuviruses and arenaviruses. the orf in ... | 1990 | 2374003 |
| gene transfer, expression and inheritance of prsv-rainbow trout-gh cdna in the common carp, cyprinus carpio (linnaeus). | a recombinant plasmid containing the rous sarcoma virus-long terminal repeat (rsv-ltr) promoter linked to rainbow trout (salmo gairdneri) growth hormone (gh) cdna was microinjected into fertilized carp eggs. genomic dna extracted from pectoral fin of individual presumptive transgenic fish was analyzed by dot blot and southern blot hybridization, using the rsv-ltr and/or the gh cdna sequences as probes. out of 365 presumptive transgenic fish analyzed, 20 individuals were found to contain prsv-rtg ... | 1990 | 2393581 |
| complementarity between the 5'- and 3'-terminal sequences of rice stripe virus rnas. | the 5' and 3' termini of four ssrna species of rice stripe virus (rsv) isolate t were sequenced. the 3' termini of the three smallest ssrnas, i.e. rnas 2, 3 and 4, had the sequence 5' gacuuugugu 3'; that of ssrna 1 had the sequence 5' gacuaugugu 3'. the 5'-terminal sequences of all four ssrnas were 5' acacaaagucc 3'. the 5'- and 3'-terminal sequences of about 20 bases of each ssrna were almost complementary to each other. it is possible that rsv rnas form panhandle structures characteristic of t ... | 1990 | 2273384 |
| primary pulmonary murine cytotoxic t lymphocyte specificity in respiratory syncytial virus pneumonia. | effector cells capable of lysing respiratory syncytial virus (rsv)-infected cells were isolated from the lungs of intranasally infected mice. an examination of their specificity showed that cytolysis was major histocompatibility complex restricted. using recombinant vaccinia viruses containing cloned rsv genes to infect target cells, balb/c (h-2d) pulmonary effector cells were shown to recognize the fusion protein (f) and to a lesser extent the nucleoprotein (n). cells specific for the major gly ... | 1990 | 2077342 |
| overexpression of avian or mouse c-jun in primary chick embryo fibroblasts confers a partially transformed phenotype. | the coding sequences of avian (quail) or murine c-jun proto-oncogenes were introduced into a non-defective retroviral vector derived from rous sarcoma virus (rsv) in which c-jun replaces v-src. primary avian fibroblasts chronically infected with either one of these viruses exhibit some phenotypic traits characteristic of rsv-transformed cells, including sustained growth in low serum medium and ability to develop colonies from single cells in agar, even though they are still of normal morphology ... | 1990 | 2123532 |
| coding strategy of rice stripe virus: major nonstructural protein is encoded in viral rna segment 4 and coat protein in rna complementary to segment 3. | the two major proteins found in plants infected with rice stripe virus (rsv), coat protein and a major nonstructural protein (major ns), were purified and their partial amino acid sequences were determined. oligonucleotides were synthesized according to the amino acid sequence information, and used as probes for northern blot analyses of four single-stranded rna species (segments 1-4) and four double-stranded rna species of rsv. the results indicated that the coding strategy of rsv was similar t ... | 1990 | 2141205 |
| fusion of rous sarcoma virus with host cells does not require exposure to low ph. | we investigated whether rous sarcoma virus (rsv) infects cells through a ph-independent or a low-ph-dependent pathway. to do this, the effects of lysosomotropic agents and acid pretreatment on rsv infectivity of, and fusion with, chicken embryo fibroblasts (cefs) were studied. high concentrations of lysosomotropic agents (ammonium chloride and monensin) did not inhibit virus infectivity: equal titers of rsv were produced in the presence and absence of these agents. similarly, low-ph pretreatment ... | 1990 | 2168989 |
| plasminogen activator gene expression is induced by the src oncogene product and tumor promoters. | secretion of urokinase-type plasminogen activator (upa) by chicken embryo fibroblasts (cef) is increased approximately 50-fold following transformation by rous sarcoma virus (rsv). using a cloned and fully sequenced chicken upa cdna probe, we have established that this increase in plasminogen activator production can be largely accounted for by an increase in cellular upa mrna. cef contained on average less than 1 molecule of upa mrna/cell, whereas rsv-cef contained 25-60 molecules/cell. the inc ... | 1990 | 2153128 |
| [ultrastructural changes in middle ear and cochlea caused by respiratory syncytial virus in guinea pigs]. | respiratory syncytial virus (rsv) was inoculated into the middle ear of guinea pigs. preyer's reflex began to decrease since 21st day. the positive rate of rsv culture from middle ear mucosa within the first week was 50%. the titer of igg antibody against rsv in sera rose gradually. electron microscopy showed enlarged rough endoplasmic reticulum, swelling mitochondria and pyknosis of nuclei in the epithelial and mononuclear cells of middle ear. the surface of corti's organ was covered with exuda ... | 1990 | 2076325 |
| protection from herpes simplex virus type 1 lethal and latent infections by secreted recombinant glycoprotein b constitutively expressed in human cells with a bk virus episomal vector. | the herpes simplex virus type 1 (hsv-1) glycoprotein b (gb-1) gene, deleted of 639 nucleotides that encode the transmembrane anchor sequence and reconstructed with the extramembrane and intracytoplasmic domains, was cloned under control of the rous sarcoma virus long terminal repeat in the episomal replicating vector prp-rsv, which contains the origin of replication and early region of the human papovavirus bk as well as a cdna for a mutant mouse dihydrofolate reductase that is resistant to meth ... | 1990 | 2152829 |
| localization of 9e3/cef-4 in avian tissues: expression is absent in rous sarcoma virus-induced tumors but is stimulated by injury. | the avian gene 9e3/cef-4, a member of the superfamily of genes that includes kc and gro, is expressed abundantly in exponentially growing cultures of chick embryo fibroblasts (cefs) and at high levels in cefs transformed with rous sarcoma virus (rsv). the product of this gene is a secreted protein that has homologies and structural similarities to inflammatory mediators. the function of 9e3 is obscure and its expression in vivo has not yet been investigated. we studied by in situ hybridization a ... | 1990 | 2155240 |
| glycyl-l-glutamine stimulates the accumulation of a12 acetylcholinesterase but not of nicotinic acetylcholine receptors in quail embryonic myotubes by a cyclic amp-independent mechanism. | myotubes prepared from the japanese quail embryo at 9 days gestation were cultivated in the presence of glycyl-l-glutamine (gly-gln, beta-endorphin c-terminal dipeptide) or glycyl-glutamic acid (gly-glu), and changes in the activity of acetylcholinesterase (ache) molecular forms and binding of 125i-alpha-bungarotoxin (alpha bgt) to cell surface nicotinic acetylcholine receptors were measured. the a12 oligomer was the major form of ache in the cultures. the activity of all molecular forms of the ... | 1990 | 2156012 |
| a study of the dimer formation of rous sarcoma virus rna and of its effect on viral protein synthesis in vitro. | the genetic material of all retroviruses examined so far is an rna dimer where two identical rna subunits are joined at their 5' ends by a structure named dimer linkage structure (dls). since the precise location and structure of the dls as well as the mechanism and role(s) of rna dimerization remain unclear, we analysed the dimerization process of rous sarcoma virus (rsv) rna. for this purpose we set up an in vitro model for rsv rna dimerization. using this model rsv rna was shown to form dimer ... | 1990 | 2155394 |
| evaluation of homology modeling of hiv protease. | the model of human immunodeficiency virus (hiv-1) protease which was based on the crystal structure of rous sarcoma virus (rsv) protease has been compared to the recently determined crystal structure of chemically synthesized hiv-1 protease. the overall difference between the model and crystal structure was 1.4 a root mean square (rms) deviation for 86 superimposed c alpha atoms. the position of the flexible flap differs in the model and six residues at the amino terminus were incorrectly placed ... | 1990 | 2158092 |
| economic and long-term benefits of ribavirin therapy on respiratory syncytial virus infection. | ribavirin is a broad-spectrum antiviral agent. administered as an aerosol, it has been shown to be clinically effective in improving the signs and symptoms of viral bronchiolitis in infancy, particularly cases due to respiratory syncytial virus (rsv). this paper reviews the evidence for economic and/or long-term clinical benefits from using ribavirin in the acute illness. there are data to suggest that use of ribavirin may lead to a reduction in therapeutic interventions and duration of hospital ... | 1990 | 2117144 |
| an epidemic of respiratory syncytial virus in elderly people: clinical and serological findings. | in 1984-1985, an outbreak of respiratory syncytial virus (rsv) infection occurred in two geriatric wards. among 68 patients (mean age +/- sd = 82.5 +/- 12.5 with respiratory signs, 52 had signs caused by rsv infection. among all patients, the clinical and serological attack rates were 61.2% and 75.0%, respectively. the most frequent clinical presentation was intensive coughing (96.1%) and fever (96.1%) associated with expectorate (63.5%). the duration of the respiratory symptoms was 5 to 7 days. ... | 1990 | 2179469 |
| acute and long-term effects of viral bronchiolitis in infancy. | about 1% of infants are admitted to hospital with acute bronchiolitis; 85% of cases are caused by infection with respiratory syncytial virus (rsv). the pathophysiological changes during the acute illness are inflammatory obstruction in the small airways with submucosal cellular infiltration, epithelial necrosis and mucous plugging; frc increases and dynamic compliance falls. failure to respond to bronchodilator drugs suggests that muscle spasm contributes relatively little to the airway narrowin ... | 1990 | 2117143 |
| serum and v-src increase the level of a ccaat-binding factor required for transcription from a retroviral long terminal repeat. | transcription from the long terminal repeat (ltr) of rous sarcoma virus (rsv) in rat 3y1 fibroblasts was dependent on the presence of serum. within 1 hr after addition of serum to a serum-deprived culture, there was a fivefold increase in the level of transcripts initiated at the ltr. this stimulation did not require synthesis of new proteins. the induction of transcription by serum was mostly dependent on two ccaat boxes in the ltr. within 1 hr after addition of serum, there was also an increas ... | 1990 | 2159932 |
| transcriptional activity of the rous sarcoma virus long terminal repeat correlates with binding of a factor to an upstream ccaat box in vitro. | the avian nuclear protein, enhancer factor 1 (ef1), binds specifically to the long terminal repeat (ltr) of rous sarcoma virus (rsv) in a region that has been implicated in enhancer/promoter function. we have characterized the in vitro binding properties of this factor from chick embryo nuclear extracts by methylation interference/protection foot-printing of the wild-type ltr and also by gel electrophoretic mobility shift assays performed on a series of ltr mutants. we find that the inverted cca ... | 1990 | 2162108 |
| [molecular basis of retrovirus adaptation: nucleotide sequence of rous sarcoma virus adapted to duck cells]. | the host range of retroviruses is rather complex and specific. it is controlled by the products of viral structural genes that interact with the determinants both on the surface and within the cell. the possibility to infect and transform duck embryo fibroblasts is shown for the prague strain of chicken rous sarcoma virus (subgroup c), though virus production in these cells is restricted. however, after the 6th passage the "adapted" virus gave the titre practically the same as it was for chicken ... | 1990 | 2162309 |
| localization by mutational analysis of transcription factor binding sequences in the u3 region of rous sarcoma virus ltr. | the transcription factor binding sequences in the u3 region of rous sarcoma virus ltr have been determined by gel retardation assays using mutant synthetic oligonucleotides. the results indicate that the factor, e2bp, specifically binds to sequences tgcaatac and tgcaacat, which are localized between nucleotides -222 to -215 and -203 to -196, respectively. this factor is present at elevated levels in avian qt6 cells compared to mouse 3t3 and rat 2 tk- cells. e2bp binds to a sequence that is simil ... | 1990 | 2161147 |
| what is the clinical role of aerosolized ribavirin? | aerosolized ribavirin is the first approved agent for the treatment of respiratory syncytial virus (rsv) infection. however, the clinical use of ribavirin is controversial. although ribavirin-treated patients appear to be subjectively improved, the benefit in terms of decreased morbidity and mortality is not well established. furthermore, the drug has been found to be teratogenic, carcinogenic, and mutagenic, depending upon the scientific model. because of its unique mode of administration, envi ... | 1990 | 2197815 |
| transformation-defective mutants with 5' deletions of the src gene are frequently generated during replication of rous sarcoma virus in established quail fibroblasts. | replication of rous sarcoma virus (rsv) in avian fibroblasts leads to the generation of replication-competent variants that are defective for cell transformation (td virus). these td variants contain deletions affecting various portions of the v-src gene. we compared the rate of td virus production in q3b cells, a quail cell line established by mutagen treatment, and in normal quail fibroblasts. twenty-five days after infection with an rsv stock containing only transforming virions, q3b cells ha ... | 1990 | 2164723 |
| detection of respiratory syncytial virus antigen in nasal washings by abbott testpack enzyme immunoassay. | we compared the new abbott testpack (tp) respiratory syncytial virus (rsv) enzyme immunoassay (eia) with cell culture and two commercial rsv eias (from abbott diagnostics and kallestad laboratories) by using split samples of fresh nasal washings from children with suspected rsv disease. two tubes of hep-2 cells were inoculated and observed for cytopathic effect for 14 days, and isolates were confirmed by immunofluorescence. the tp eia was performed by following the manufacturer's instructions. s ... | 1990 | 2199500 |
| identification of a third protein factor which binds to the rous sarcoma virus ltr enhancer: possible homology with the serum response factor. | we have identified a new protein factor (efiii) in nuclear extracts of quail fibroblasts and chick embryos which binds specifically in vitro to a 26-bp region of the rous sarcoma virus (rsv) long terminal repeat (ltr) enhancer. the efiii binding site in the rsv ltr exhibits a strong sequence homology to the serum response element (sre). the sre is a 22-bp cis-acting dna sequence element, first identified upstream of the human c-fos gene, which can confer serum inducibility to heterologous promot ... | 1990 | 2152992 |
| mediation of wound-related rous sarcoma virus tumorigenesis by tgf-beta. | in rous sarcoma virus (rsv)-infected chickens, wounding leads to tumor formation with nearly 100% frequency in tissues that would otherwise remain tumor-free. identifying molecular mediators of this phenomenon should yield important clues to the mechanisms involved in rsv tumorigenesis. immunohistochemical staining showed that tgf-beta is present locally shortly after wounding, but not unwounded controls. in addition, subcutaneous administration of recombinant transforming growth factor-beta 1 ( ... | 1990 | 2163544 |
| distinct and different effects of the oncogenes v-myc and v-src on avian sympathetic neurons: retroviral transfer of v-myc stimulates neuronal proliferation whereas v-src transfer enhances neuronal differentiation. | immature avian sympathetic neurons are able to proliferate in culture for a limited number of divisions albeit expressing several neuron-specific properties. the effect of avian retroviral transfer of oncogenes on proliferation and differentiation of sympathetic neurons was investigated. primary cultures of 6-d-old quail sympathetic ganglia, consisting of 90% neuronal cells, were infected by myelocytomatosis virus (mc29), which contains the oncogene v-myc, and by the v-src-containing rous sarcom ... | 1990 | 2161856 |
| malignant transformation of human endometrial stromal cells by transfection of c-myc: effects of prsvneo cotransfection and treatment with mnng. | stromal cells isolated from normal human endometrium were cotransfected in primary culture with psvc-myc, a plasmid containing a truncated c-myc gene regulated by simian virus 40 promoter, and prsv neo, a plasmid containing a neomycin resistance gene regulated by rous sarcoma virus (rsv) promoter. these cells demonstrated properties of transformed cells in vitro, including altered morphology, focus formation, anchorage-independent growth, chromosomal alterations, and tumor formation in athymic m ... | 1990 | 2171549 |
| down regulation by p60v-src of genes specifically expressed and developmentally regulated in postmitotic quail neuroretina cells. | the avian neuroretina (nr) is composed of photoreceptors and different neurons that are derived from proliferating precursor cells. neuronal differentiation takes place after terminal mitosis. we have previously shown that differentiating nr cells can be induced to proliferate by infection with rous sarcoma virus (rsv) and that cell multiplication requires expression of a functional v-src gene. we speculated that the quiescence of nr cells could be determined by specific genes. cell proliferatio ... | 1990 | 2162475 |
| pp60v-src association with the cytoskeleton induces actin reorganization without affecting polymerization status. | the mechanism by which rous sarcoma virus (rsv) induces a reorganization of actin and its associated proteins and a reduction in microfilament bundles is at present poorly understood. to examine the relationship between the organization of the microfilament system and the polymerization state of actin after transformation, we have investigated these changes in a rat-1 cell line transformed by la29, a temperature-sensitive (ts) mutant of rsv. parallel immunofluorescence and biochemical analysis d ... | 1990 | 2167224 |
| morphological aspects of inhibition of transformation of rat kidney cells infected with a temperature-sensitive mutant of the rous sarcoma virus by rat fibroblast interferon. | normal rat kidney cells (nrk) infected with a temperature-sensitive (ts) mutant of rous sarcoma virus (rsv) at the permissive temperature (33 degrees c) exhibit the morphological and growth characteristics of the transformed state. by the use of this system, it has been shown that the addition of rat fibroblast interferon to infected cells grown at a nonpermissive temperature (38 degrees c) prevents re-transformation when they are introduced to the permissive temperature. merely treating cells a ... | 1990 | 2168311 |
| the effects of 3-deazaguanine on rous sarcoma virus transformation. | an expression of cellular transformation in chicken embryo fibroblast infected with rous sarcoma virus (rsv) was inhibited by 3-deazaguanine (3-dg). re-transformation of normal rat kidney cells infected with a temperature-sensitive mutant of rsv was suppressed by 3-dg when the cells were shifted from the non-permissive temperature (38 degrees c) to the permissive temperature (33 degrees c). removal of the inhibitor enabled the cells to revert to the transformed state. finally, 3-dg had little or ... | 1990 | 2168314 |
| sequence specificity of mrna n6-adenosine methyltransferase. | the sequence specificity of chicken mrna n6-adenosine methyltransferase has been investigated in vivo. localization of six new n6-methyladenosine sites on rous sarcoma virus (rsv) virion rna has confirmed our extended consensus sequence for methylation: rgacu, where r is usually a g (7/12). we have also observed a (2/12) and u (3/12) at the -2 position (relative to m6a at +1) but never a c. at the +3 position, the u was observed 10/12 times; an a and a c were observed once each in weakly methyla ... | 1990 | 2173695 |
| acute lower respiratory tract infection due to virus among hospitalized children in dhaka, bangladesh. | the nature of acute lower respiratory tract infection (alri) in hospitalized children and of the associated viral agents was assessed in a study of 601 children less than 5 years old over a 24-month period. of these children, 80% were less than 24 months of age and the ratio of boys to girls was 1.7:1. pneumonia (86.5% of cases) was the most frequently observed clinical manifestation. shedding of virus was detected in 21.1% of the children; the highest rate occurred in infants 0-5 months old (27 ... | 1990 | 2176737 |
| fusion of rous-sarcoma-virus-transformed rat cells to morphologically normal human or rat cells results in transcriptional suppression of the provirus that depends on its chromosomal integration site. | the fusion of a rous sarcoma virus (rsv)-transformed rat fibroblast clone to at least 2 different human cell types reproducibly produces phenotypically normal hybrids. analysis of such hybrids reveals that proviral silence is the result of transcriptional down-regulation, presumably by a trans-acting human molecule. furthermore, this phenomenon seems to be strongly influenced by the proviral chromosomal integration site and its imposition may entail a mechanism that is required only transiently. | 1990 | 2166711 |
| [serologic response measured by complement fixation in children with acute respiratory infection]. | the aim of this work was to evaluate the sensitivity of complement fixation for serological diagnosis of lower acute respiratory infections (ari) in small children in comparison with direct methods such as indirect immunofluorescence (iif) on nasopharyngeal aspirate and tissue culture isolation. sera from children under 5 year of age with ari were studied by complement fixation for 6 respiratory viruses (respiratory syncytial virus (rsv), adenovirus, influenza a and b and parainfluenza 1 and 3). ... | 1990 | 2287713 |
| serodiagnosis of respiratory syncytial virus (rsv) infection in children as measured by detection of rsv-specific immunoglobulins g, m, and a with enzyme-linked immunosorbent assay. | the diagnostic value of an enzyme-linked immunosorbent assay for detection of respiratory syncytial virus (rsv)-specific immunoglobulin g (igg), igm, and iga in sera from infants and children with proven rsv infection, from a control group, and from patients with symptoms of viral respiratory disease was analyzed. compared to virus isolation and rsv antigen detection methods, the sensitivity of this assay was 87% and the specificity was 79%. for igg alone, these were 45 and 92%, for igm alone th ... | 1990 | 2298874 |
| transactivation of the rous sarcoma virus long terminal repeat promoter by marek's disease virus. | transient expression of chloramphenicol acetyltransferase (cat) was used to study marek's diseases virus (mdv)-mediated transactivation of the rous sarcoma virus long terminal repeat (rsv-ltr) promoter. cotransfection experiments in primary avian cells were conducted using mdv high-molecular-weight dna and plasmid prsvcat. increased cat activity, relative to controls, was consistently observed in the presence of mdv. enhanced cat activity, expressed via the rsv-ltr promoter, was strictly depende ... | 1990 | 2173259 |
| incorporation of chimeric gag protein into retroviral particles. | the product of the rous sarcoma virus (rsv) gag gene, pr76gag, is a polyprotein precursor which is cleaved by the viral protease to yield the major structural proteins of the virion during particle assembly in avian host cells. we have recently shown that myristylated forms of the rsv gag protein can induce particle formation with very high efficiency when expressed in mammalian cells (j. w. wills, r. c. craven, and j. a. achacoso, j. virol. 63:4331-4343, 1989). we made use of this mammalian sys ... | 1990 | 2166812 |
| a seroepidemiologic study of bovine respiratory syncytial virus (brsv) in turkey. | in this study, a survey of cattle for neutralizing antibodies to respiratory syncytial virus (rsv) in turkey is presented. 490 serum samples were collected from several state farms and some private flocks in different regions of turkey. the serologic examination was done by the microtiter serum-neutralization technique. in this report, a positive serum antibody titer at dilutions of 1:2 was demonstrated in 226 (46.12%) of the 490 sera tested. | 1990 | 2311535 |
| viral aetiology of acute respiratory infections in children in north india. | two hundred and thirty children clinically diagnosed as suffering from acute respiratory infection were tested for four major groups of viral aetiological agents, i.e. influenza para-influenza, respiratory syncytial virus (rsv) and adenoviruses using indirect immunofluorescence technique. at least one of the respiratory viruses was identified in 51 (22 per cent) specimens, which included influenza a in 6 (3 per cent), influenza b in 3 (1 per cent), para-influenza type 1 in 3 (1 per cent), para-i ... | 1990 | 2313777 |
| a retroviral promoter is sufficient to convert proto-src to a transforming gene that is distinct from the src gene of rous sarcoma virus. | the src genes of four natural isolates of avian sarcoma viruses differ from cellular proto-src in two genetic substitutions: the promoter of the cellular gene is replaced by a retroviral counterpart, and at least six codons from the 3' terminus are replaced by retroviral or heterologous cell-derived elements. since virus constructs with a complete proto-src coding region failed to transform avian cells but acquired transforming function by point mutations of various codons, it has been proposed ... | 1990 | 2174554 |
| regulation of integrin mobility and cytoskeletal association in normal and rsv-transformed chick embryo fibroblasts. | the mobility of the integrin receptor in trypsinised chick embryo fibroblasts (cef) was investigated using the csat monoclonal antibody. the binding of csat to trypsinised cef followed by incubation at 37 degrees c resulted in patching and then capping of the receptor. this capping was dependent on cellular metabolism, since agents such as sodium azide or 2-deoxyglucose inhibited the process. whereas about 95% of unclustered integrin was soluble in nonidet p40-containing buffers, after capping m ... | 1990 | 2177479 |