Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| natural hybridization between diploid crucian carp species and genetic independence of triploid crucian carp elucidated by dna markers. | the population structure of genus carassius in lake koyama, southeast japan, was analyzed by genetic markers as microsatellite dna, mtdna rflp and isozymes. based on the ploidy level and morphological analysis, four carassius groups were detected. the triploid group was identified as ginbuna (c. langsdorfii). in the diploid group, nagabuna (c. burugeri sp) and gengoroubuna, (c. cuvieri) were identified. remaining diploid individuals had morphological traits that were intermediate between nagabun ... | 2000 | 18494591 |
| physical constraints on body size in teleost embryos. | all members of the subphylum "vertebrata" display the characteristics of the vertebrate body plan. these characteristics become apparent during the phylotypic period, in which all vertebrate embryos have a similar body shape and internal organization. phylogenetic constraints probably limit the morphological variation during the phylotypic period. physical laws, however, also limit growth and morphogenesis in embryos. we investigated to what extent oxygen availability-as a physical constraint-mi ... | 2000 | 10772852 |
| brain blood flow during hypercapnia in fish: no role of nitric oxide. | very little is known about the regulation of cerebral blood flow (cbf) in lower vertebrates, especially fish. in mammals, hypercapnia causes cerebral vasodilation and increased cbf through mechanisms that involve the production of nitric oxide (no). we have used epi-illumination microscopy in vivo to observe effects of hypercapnia on venular erythrocyte velocity, used as an index of cbf velocity, in rainbow trout (oncorhynchus mykiss) and crucian carp (carassius carassius). rainbow trout exposed ... | 2000 | 10700569 |
| alarm reaction in the crucian carp is mediated by the medial bundle of the medial olfactory tract. | experiments were performed to determine which bundles of the olfactory tracts were essential for mediating alarm reaction in crucian carp (carassius carassius l.). the fish were maintained in physiological saline after surgery to preserve the remaining tracts and postoperative inspections revealed the functionality of the intact tracts. operations on the tracts were performed symmetrically on both sides. sham-operated and non-operated fish showed the typical alarm behaviour of fast swimming to t ... | 2000 | 10668000 |
| genetic evidence for gonochoristic reproduction in gynogenetic silver crucian carp (carassius auratus gibelio bloch) as revealed by rapd assays. | sex evolution has been a debating focus in evolutionary genetics. in lower vertebrates of reptiles, amphibians, and fish, a species or a bioform reproduces either sexually or asexually but never both. a few species were found to consist of all females in fish. these all-female species can propagate by asexual reproduction modes, such as gynogenesis and hybridogenesis. however, the coexistence of sexuality and asexuality in a single species was recently noted only in a cyprinid fish silver crucia ... | 2000 | 11080373 |
| endocrine disruption by hexachlorobenzene in crucian carp (carassius auratus gibelio). | 2000 | 11014838 | |
| the homologue of mannose-binding lectin in the carp family cyprinidae is expressed at high level in spleen, and the deduced primary structure predicts affinity for galactose. | mannose-binding lectin (mbl) participates in the innate immune system as an activator of the complement system and as an opsonin after binding to certain carbohydrate structures on microorganisms. we isolated and characterized cdna transcripts encoding an mbl homologue from three members of the carp family cyprinidae, the zebrafish danio rerio, the goldfish carassius auratus, and the carp cyprinus carpio. the carp and zebrafish transcripts contain two polyadenylation sites and rt-pcr on mrna fro ... | 2000 | 11003389 |
| extinction risk of a density-dependent population estimated from a time series of population size. | environmental threats, such as habitat size reduction or environmental pollution, may not cause immediate extinction of a population but shorten the expected time to extinction. we develop a method to estimate the mean time to extinction for a density-dependent population with environmental fluctuation. we first derive a formula for a stochastic differential equation model (canonical model) of a population with logistic growth with environmental and demographic stochasticities. we then study an ... | 2000 | 10816359 |
| comparing risk factors for population extinction. | extinction risk of natural populations of animals and plants is enhanced by many different processes, including habitat size reduction and toxic chemical exposure. we develop a method to evaluate different risk factors in terms of the decrease in the mean extinction time. we choose a population model with logistic growth, environmental and demographic stochasticities with three parameters (intrinsic growth rate r, carrying capacity k, and environmental noise sigma(2)(e)). the reduction in the ha ... | 2000 | 10816358 |
| analysis of genetic heterogeneity among five gynogenetic clones of silver crucian carp, carassius auratus gibelio bloch, based on detection of rapd molecular markers. | the gynogenetic silver crucian carp, carassius auratus gibelio, is a unique model system for studying evolutionary genetics and selective breeding, owing to its specific genetic background and reproductive modes. five gynogenetic clones were analyzed by the random amplified polymorphic dna (rapd) technique, using 30 10-nucleotide-long primers. twenty-six primers produced well-amplified dna fragments with reproducible banding patterns, and 24 primers were polymorphic. nearly identical banding pat ... | 2000 | 10773687 |
| immunohistochemical localization of c-rfamide, a fmrf-related peptide, in the brain of the goldfish, carassius auratus. | carassius rfamide (c-rfa) is a novel peptide found in the brain of the japanese crucian carp. it has been demonstrated that mrna of c-rfa is present in the telencephalon, optic tectum, medulla oblongata, and proximal half of the eyeball in abundance. immunohistochemical methods were employed to elucidate the distribution of the peptide in the brain of the goldfish (carassius auratus) in detail. c-rfaimmunoreactive perikarya were observed in the olfactory bulb, the area ventralis telencephali par ... | 2000 | 18522460 |
| horseradish peroxidase binding to intestinal brush-border membranes of cyprinus carpio. identification of a putative receptor. | morphologic studies have shown that the classic endocytosis tracer horseradish peroxidase (hrp) is actively internalized by vesicular transport in the carp intestine, suggesting the existence of specific binding sites in the apical membrane of enterocytes. the aim of the present study was to develop an in vitro binding assay using isolated carp intestinal brush-border membranes (bbm) to demonstrate and characterize these specific hrp binding sites. the results obtained show that hrp binding to b ... | 2000 | 11074599 |
| effect of the pesticide deltamethrin on the mauthner cells of lake balaton fish. | deltamethrin, a synthetic pesticide [(s)alpha-cyano-3-phenoxybenzyl-(1r)-cis-3-(2.2-dibromovinyl)-2,2-dim ethylcyclopropane-carboxylate] used for extermination of mosquitoes on the shores of lake balaton, has been found to induce severe impairments of the nervous system of several lake balaton fish, such as carp (cyprinus carpio), goldfish (carassius auratus gibelis bloch), eel (anguilla anguilla) and wels (silurus glanis). it has been shown that deltamethrin, in a concentration of 1 microgram/l ... | 2000 | 10894124 |
| organochlorine residues in european eel (anguilla anguilla), crucian carp (carassius carassius) and catfish (ictalurus nebulosus) from vaccarès lagoon (french national nature reserve of camargue) - effects on some physiological parameters. | european eels (anguilla anguilla), crucian carps (carassius carassius) and catfish (ictalurus nebulosus) were collected in three coastal locations of the vaccarès lagoon (french national nature reserve of camargue). the purpose of this paper is to report results of the first biomonitoring investigation in fish living in this protected coastal wetland. residues of organochlorine (oc) contaminants (i.e. sigmapcbs, gammahch, hcb, dieldrin, pp'-dde) were determined in hepatic and muscular tissues, i ... | 2000 | 10794830 |
| comparative study of acetylcholinesterase and butyrylcholinesterase in brain and serum of several freshwater fish: specific activities and in vitro inhibition by ddvp, an organophosphorus pesticide. | a comparative study of specific activities and in vitro inhibition of brain and serum acetylcholinesterase (ache; ec 3.1.1.7) and serum butyrylcholinesterase (bche; ec 3.1.1.8) by ddvp, an organophosphorus pesticide, was conducted in 11 freshwater teleost species belonging to four families (cyprinidae; common carp cyprinus carpio, bream abramis brama, blue bream a. ballerus, white bream blicca bjoerkna, roach rutilus rutilus, bleak alburnus alburnus, ide leuciscus idus; percidae: perch perca flu ... | 2000 | 11246494 |
| isolation and characterization of glycosylphosphatidylinositol-anchored, mucin-like surface glycoproteins from bloodstream forms of the freshwater-fish parasite trypanosoma carassii. | wild and farmed freshwater fishes are widely and heavily parasitized by the haemoflagellate trypanosoma carassii. in contrast, common carp, a natural host, can effectively control experimental infections by the production of specific anti-parasite antibodies. in this study we have identified and partially characterized mucin-like glycoproteins which are expressed in high abundance [(6. 0+/-1.7)x10(6) molecules.cell(-1)] at the surface of the bloodstream trypomastigote stage of the parasite. the ... | 2000 | 10642530 |
| a thyrotropin-like molecule from the pituitary of an indian freshwater murrel: comparison of its biological activity with other thyrotropins. | murrel pituitary thyrotropin-like molecule (mtsh) was purified to homogeneity with the help of a convenient and sensitive in vitro assay system where addition of this material to the thyroid follicle incubation stimulated thyroxine (t(4)) secretion into the medium. pituitary extract of a freshwater murrel, channa punctatus, was solvent extracted to obtain glycoprotein enriched fraction. this was subjected to sephadex g-100 gel filtration and eluate of void volume (peak i) showed strong tsh activ ... | 2000 | 11790339 |
| concentrations and frequencies of polychlorinated biphenyl congeners in a native american population that consumes great lakes fish. | polychlorinated biphenyl congener profiles were examined in serum samples from 61 native american (ojibwa) volunteers who regularly consumed fish harvested from the great lakes region. a total of 93 peaks are reported which represent 126 individual chlorobiphenyls. | 2000 | 11192460 |
| muscle regulatory factor gene: zebrafish (danio rerio) myogenin cdna. | myogenin is one of the basic helix-loop-helix proteins that regulate muscle-specific gene expression. using reverse transciption-polymerase chain reaction (rt-pcr), 5'- and 3'-rapid amplification of cdna ends (race), zebrafish myogenin cdna was cloned from mrna of embryos at 10-96 h post-fertilization. the cdna, at 1384 base pairs (bp), contained a 771-bp open reading frame with 113- and 500-bp flanking regions at the 5'- and 3'-ends, respectively. the deduced amino acid sequences of zebrafish m ... | 2000 | 11126756 |
| records of the bird capillariid nematode ornithocapillaria appendiculata (freitas, 1933) n. comb. from freshwater fishes in mexico, with remarks on capillaria patzcuarensis osorio-sarabia et al., 1986. | re-examination of capillariid specimens collected from the freshwater fish chirostoma estor jordan from lake pátzcuaro in 1985-1986 and deposited as paratypes of capillaria patzcuarensis osorio-sarabia, pérez-ponce de león & salgado-maldonado, 1986 showed that their morphology was in contradiction with the description of this species and, in fact, they could be identified as the species originally described as c. appendiculata freitas, 1933 from cormorants phalacrocorax brasilianus (gm.) in braz ... | 2000 | 10682923 |
| structure-fluorescence correlations in a single tryptophan mutant of carp parvalbumin: solution structure, backbone and side-chain dynamics. | heterogeneous fluorescence intensity decays of tryptophan in proteins are often rationalized using a model which proposes that different rotameric states of the indole alanyl side-chain are responsible for the observed fluorescence lifetime heterogeneity. we present here the study of a mutant of carp parvalbumin bearing a single tryptophan residue at position 102 (f102w) whose fluorescence intensity decay is heterogeneous and assess the applicability of a rotamer model to describe the fluorescen ... | 2000 | 10704313 |
| enhancing effect of 5 alpha-cyprinol sulfate on mucosal membrane permeability to sodium ampicillin in rats. | effect of 5 alpha-cyprinol sulfate, a bile alcohol sulfate specific to carp bile, on rectal membrane permeability to sodium ampicillin (amp na) was examined in rats. amp na is not easily absorbed through rat rectal membrane without aid. 5 alpha-cyprinol sulfate significantly enhanced the rectal membrane permeability to amp na even at a low concentration (6.25 mm), though sodium taurocholate needed a higher concentration (25 mm). co-administration of phosphatidylcholine significantly suppressed t ... | 2000 | 10704893 |
| two metallothioneins in the fresh-water fish, crucian carp (carassius cuvieri): cdna cloning and assignment of their expression isoforms. | two metallothionein cdnas (mt-a and mt-b) in the fresh-water fish crucian carp (carassius cuvieri temminck et schlegel) were cloned. sequence analysis of both cdnas gave the structure of a coding region corresponding to 60 amino acid residues. the homology of their deduced amino acid sequences was completely conserved at the positions of the cysteine residue, but a significant difference existed in the size of their 3'-untranslated regions (130 base pairs for mt-a and 280 base pairs for mt-b). d ... | 2000 | 10706375 |
| characterization of parameters for in vitro culture of isolated ovarian follicles of greenback flounder rhombosolea tapirina. | isolated ovarian follicles of greenback flounder rhombosolea tapirina were incubated with a variety of gonadotropins (gths) and steroid precursors for periods of up to 42 h, and levels of free and glucuronated testosterone (t) and 17beta-estradiol (e(2)) in the medium, and free t and e(2) from inside follicles were measured by ria. short incubations (6 h) generated increases in t and e(2) in response to steroid precursors, but not human chorionic gth (hcg), or salmon or carp gth. at incubation t ... | 2000 | 11064285 |
| evaluation of the genotoxic and cytochrome p450 monooxygenase-inhibitory potential of dicuran on procaryotic and eucaryotic test systems. | the effect of the herbicide dicuran 500 fl (formulated product) on the phenotypical and genotypical changes in procaryotic and eucaryotic organisms was investigated using short-term tests for detecting genotoxins. since pesticides discharged in the water environment can modulate the mixed-function monooxygenases (mfo) detoxification system of water organisms, the in vivo and in vitro effects of dicuran on hepatic cytochrome p450 (cyt p450) monooxygenase activities were also examined in juvenile ... | 2000 | 11069017 |
| genomic organization and the promoter region of the round-spotted pufferfish (tetraodon fluviatilis) cdc37 gene. | the cdc37 gene was isolated from a round-spotted pufferfish genomic library and characterized. this gene is composed of nine exons spanning 3.5 kb. exon 1 contains the 5'-untranslated region and exon 2 contains the putative translation initiation site. by 5'-race (rapid amplication of cdna ends) and sequence analysis, we deduced the promoter region for the cdc37 gene and found that it does not contain typical tata or ccaat box. the 1.8 kb dna fragment upstream of the putative transcription initi ... | 2000 | 11072077 |
| determination of 4-nonylphenol, nonylphenol monoethoxylate, nonylphenol diethoxylate and other alkylphenols in fish and shellfish by high-performance liquid chromatography with fluorescence detection. | a simple and highly sensitive method is described for the hplc determination of 4-nonylphenol (np), 4-nonylphenol mono- (np1eo) and diethoxylates (np2eo) in fish and shellfish together with bisphenol a (bpa), 4-tert.-butylphenol (bp) and 4-tert.-octylphenol (op). the np, np1eo, np2eo and other alkylphenols in the samples are extracted with acetonitrile and the lipid in the sample extract is eliminated by partitioning between hexane and acetonitrile. after florisil pr clean-up the sample extract ... | 2000 | 11076083 |
| light-adaptive role of nitric oxide in the outer retina of lower vertebrates: a brief review. | the role of nitric oxide (no) as a novel neurochemical mechanism controlling light adaptation of the outer retina is discussed by considering mainly published results. the emphasis is on the retinae of fishes and amphibia, but some data from the mammalian (rabbit) retinae have also been included for completeness. in the fish retina, application of no donors in the dark caused light-adaptive photomechanical movements of cones. the normal effect of light adaptation in inducing cone contractions wa ... | 2000 | 11079398 |
| molecular cloning of carp (cyprinus carpio) leucocyte cell-derived chemotaxin 2, glia maturation factor beta, cd45 and lysozyme c by use of suppression subtractive hybridisation. | 2000 | 11081442 | |
| physiological induction of a beta-adrenergic receptor kinase inhibitor transgene preserves ss-adrenergic responsiveness in pressure-overload cardiac hypertrophy. | transgenic mice with constitutive myocardium-targeted expression of a peptide inhibitor of the ss-adrenergic receptor kinase (ssarkct) have increased in vivo cardiac function and enhanced ss-adrenergic receptor (ssar) responsiveness. | 2000 | 11094043 |
| addition of signal leader sequences to the n-termini of olfactory receptor proteins enhances their expression in xenopus oocytet. | several recent papers have reported the difficulties in expressing olfactory receptor proteins (ors) in heterologous systems, and proposed that some sequences in ors have negative effects on their efficient expression. to obtain an efficient expression system of ors, we modified n-terminal sequences of ors through the addition of exogenous sequences. three kinds of sequences, designated as 5ht, v, and vl, were used. 5ht and v corresponded to the signal leader (sl) sequences of 5ht 3r and vipr, r ... | 2000 | 10993157 |
| cyclooxygenase-derived products, rather than nitric oxide, are endothelium-derived relaxing factor(s) in the ventral aorta of carp (cyprinus carpio). | in some fish blood vessels, the existence of a no (nitric oxide) system has been reported. we examined the possibility that this no system acts as an endothelium-derived relaxing factor (edrf) in carp aorta using the carp aorta alone and in a combined carp-rat aorta donor-detector system. use of the typical no stimulating agent in mammal acetylcholine (ach) only induced constriction of the carp aorta. this response was not modified by denudation or by no synthesis inhibition with n-nitro-l-argin ... | 2000 | 10996821 |
| enhancement of catalytic efficiency by the combination of site-specific mutations in a carbonic anhydrase-related protein. | a single mutation, involving the replacement of an arginine residue with histidine to reconstruct a zinc-binding site, suffices to change a catalytically inactive murine carbonic anhydrase-related protein (carp) to an active carbonic anhydrase with a co2-hydration turnover number of 1.2 x 104 s-1. further mutations, leading to a more 'carbonic anhydrase-like' active-site cavity, results in increased activity. a quintuple mutant having his94, gln92, val121, val143, and thr200 (human carbonic anhy ... | 2000 | 10998050 |
| color-opponent responses of small and giant bipolar cells in the carp retina. | the physiological and morphological properties of color-opponent bipolar cells in the carp retina were studied. fifty nine off-center bipolar cells and 63 on-center bipolar cells out of about 500 total bipolar cells recorded showed color-opponent responses. the off-center color-opponent bipolar cells were classified into three subgroups according to their spectral and spatial responses. fifty off-center color-opponent cells responded with depolarization to a blue light spot and with hyperpolariz ... | 2000 | 11016579 |
| a case with 47,xxy,del(11)(q23) karotype-coexistence of jacobsen and klinefelter syndromes. | a case with 47,xxy, del(11)(q23) karyotype-coexistence of jacobsen and klinefelter syndromes: a two-year-old dysmorphic male child was found to have 47,xxy,del(11)(q23) karyotype. domination of the clinical features of jacobsen syndrome was observed: mild mental retardation, trigonocephaly, ptosis, downward slanting palpebral fissures, low set ears, carp-shape mouth and micrognathia. transient thrombocytopenia and leukopenia were also present. over the following five years gynecomastia and eunuc ... | 2000 | 11043435 |
| concentrations of some heavy metals in water, sediment and fish species from the atatürk dam lake (euphrates), turkey. | concentrations of heavy metals (cd, co, cu, fe, hg, mn, mo, ni, pb and zn) were measured in the water, sediment and fish species (acanthobrama marmid, chalcalburnus mossulensis, chondrostoma regium, carasobarbus luteus, capoetta trutta and cyprinus carpio) from the atatürk dam lake, turkey. among the heavy metals studied cd, co, hg, mo and pb were not detected in water, sediments and fish samples, while ni was undetectable levels in fish samples. levels of cu, fe, mn and zn varied depending on d ... | 2000 | 11057573 |
| cloning, sequencing, and analysis of expression of a second il-1beta gene in rainbow trout (oncorhynchus mykiss). | the full-length sequence of a second il-1beta gene (il-1beta2) in rainbow trout (oncorhynchus mykiss) has been obtained. as with the first il-1beta gene, il-1beta2 is organized into six exons/five introns. there are only small differences in their intron/exon sizes, with the exception of intron 3, which is 334 bp smaller in il-1beta2. the transcript encoded by the il-1beta2 gene contains a 5' untranslated region (utr) of 121 bp, followed by a 762-bp open reading frame and a 518-bp 3'utr. the 3'u ... | 2000 | 11061285 |
| chronic toxic effects of quinalphos on some biochemical parameters in labeo rohita (ham.). | the effect of exposure to sublethal concentrations of the organophosphate pesticide, quinalphos (1.12, 0.22 mg/l) on biochemical parameters of muscle and enzyme activities in brain, liver and kidney of the indian major carp, labeo rohita was studied after 15, 30 and 45 days. the muscle protein and rna levels decreased whereas dna levels and acid phosphatase were elevated. similarly, alkaline phosphatase was depleted. the brain acetyl cholinesterase activity was decreased most (-75.43%) in 1.12 m ... | 2000 | 10713464 |
| design and solution structure of a well-folded stack of two beta-hairpins based on the amino-terminal fragment of human granulin a. | four amino acid substitutions were introduced into a peptide corresponding to the amino-terminal subdomain (30-31 residues) of human granulin a (hga) in order to assess the contributions of a hydrophobic framework and other interactions to structure stabilization of the stack of two beta-hairpins. the resulting hybrid peptide, hga 1-31 (d1v, k3h, s9i, q20p) with four free cysteines, spontaneously formed a uniquely disulfide-bonded isomer with an expected [1-3, 2-4] disulfide pairing pattern. thi ... | 2000 | 10715107 |
| pathological and biochemical characterization of microcystin-induced hepatopancreas and kidney damage in carp (cyprinus carpio). | mass occurrences of cyanobacteria, due to their inherent capacity for toxin production, specifically of microcystins (mc), have been associated with fish kills worldwide. the uptake of mc-lr and the sequence of pathological and associated biochemical changes was investigated in carp (cyprinus carpio) in vivo over 72 h. carp were gavaged with a single sublethal bolus dose of toxic microcystis aeruginosa (pcc 7806) amounting to an equivalent of 400 microg mc-lr/kg body wt. damage of renal proximal ... | 2000 | 10739746 |
| surface ultrastructure of metagonimus takahashii metacercariae and adults. | a scanning electron microscopic study was performed on the surface ultrastructure of metacercariae and adults of metagonimus takahashii. metacercariae were collected from the scale of crucian carp (carassius auratus), and adult flukes were harvested 1-4 weeks after infection to rats. in excysted metacercariae, the oral sucker had type i (numerous) and type ii (seven in total) sensory papillae. tegumental spines were dense and digitated into 5-7 points on the surface anterior to the ventral sucke ... | 2000 | 10743353 |
| a novel truncated isoform of the mannose-binding lectin-associated serine protease (masp) from the common carp (cyprinus carpio). | the mannose-binding lectin (mbl) and mbl-associated serine proteases (masps) play crucial roles in activation of the lectin pathway of the complement system. mammals and xenopus possess two distinct masps, masp1 and masp2, with different substrate specificity. recently, a truncated form named map19 or smap, composed of n-terminal c1r/c1s/uegf/bone morphogenetic protein (cub)-1 and epidermal growth factor domains of masp2, has been shown to be generated by alternative polyadenylation and splicing ... | 2000 | 10752628 |
| gonadotropin-releasing hormone stimulates thyroid activity in a freshwater murrel, channa gachua (ham.), and carps, catla catla (ham.) and cirrhinus mrigala (ham.). | injections of mammalian gnrh (mgnrh), salmon gnrh (sgnrh), and a homologous murrel, channa punctatus, gnrh (cgnrh) to a murrel, channa gachua, and a carp, catla catla, at a dose of 1 microg/250 g body wt significantly increased plasma thyroxine (t4) levels above control. piscine gnrhs (sgnrh and cgnrh) had significantly greater stimulatory effects compared with mgnrh. to observe whether this stimulatory effect by gnrhs is direct or indirect, thyroid follicles were isolated from hypobranchial mus ... | 2000 | 10764556 |
| viremia-associated ana-aki-byo, a new viral disease in color carp cyprinus carpio in japan. | a new virus disease that displays dermal ulceration and high mortality has been occurring since 1996 in color carp cyprinus carpio reared in warm water in japan. in histological examinations, initial erosive lesions displayed necrosis, hemorrhage and fibrin deposition in the dermal loose connective tissue and were accompanied by the partial destruction of the epidermis. developed ulcerative lesions involved the lateral musculature with bacterial invasions. in visceral organs, necrotic cells were ... | 2000 | 10768286 |
| a study of sequential histopathology of trypanoplasma borreli (protozoa: kinetoplastida) in susceptible common carp cyprinus carpio. | the tissue response of common carp cyprinus carpio to the kinetoplastid blood parasite trypanoplasma borreli laveran & mesnil, 1901 was investigated during a laboratory infection of a highly susceptible carp line. with the development of the parasitaemia an increased proliferation of the lymphoid renal interstitial tissue was induced, which resulted in a progressive depression and deterioration of renal tubules. in heavily infected carp at days 20 to 28 post inoculation (pi), a tubulonephrosis, ... | 2000 | 10768290 |
| up-regulation of natriuretic peptides in the ventricle of csx/nkx2-5 transgenic mice. | a cardiac homeobox-containing gene csx/nkx2-5, which is essential for cardiac development, is abundantly expressed in the adult heart as well as in the heart primordia. targeted disruption of this gene results in embryonic lethality due to abnormal heart morphogenesis. to elucidate the role of csx/nkx2-5 in the adult heart, we generated transgenic mice which overexpress human csx/nkx2-5. the transgene was expressed abundantly in the heart and the skeletal muscle. mrna levels of several cardiac g ... | 2000 | 10772952 |
| uptake and processing of biofilm and free-cell vaccines of aeromonas hydrophila in indian major carps and common carp following oral vaccination--antigen localization by a monoclonal antibody. | uptake and processing of biofilm (bf) and free-cell (fc) vaccines of aeromonas hydrophila were studied in the indian major carps catla catla catla, and rohu labeo rohita and in the common carp cyprinus carpio following a single dose oral vaccination of 10(11) cfu g(-1) fish. fish were sampled at 0.5, 1, 3, 6, 12, 24 h and at 2, 3, 5 and 10 d following vaccination and antigen localization was studied in the gut, kidney and spleen employing monoclonal antibody based immunofluorescence and immunope ... | 2000 | 11145450 |
| an immunocytochemical study of the pituitary gland of the white seabream (diplodus sargus). | the adenohypophysis of the white seabream (diplodus sargus) was studied using histochemical and immunocytochemical techniques. the adenohypophysis was composed of rostral pars distalis, proximal pars distalis and pars intermedia. prolactin (anti-chum salmon prolactin positive) and adrenocorticotropic (anti-human acth positive) cells were found in the rostral pars distalis. prolactin cells were organized into follicles, while acth cells were arranged in cords around neurohypophyseal tissue branch ... | 2000 | 11254089 |
| preparation of neoglycoprotein from carp myofibrillar protein and alginate oligosaccharide: improved solubility in low ionic strength medium. | alginate oligosaccharide (ao) was conjugated with carp myofibrillar protein (mf) by using the controlled maillard reaction, and the change in the solubility of mf in low ionic strength media as affected by the glycosylation was investigated. ao was prepared by degrading sodium alginate using alginate lyase, which was purified from the culture supernatant of pseudoalteromonas elyakovii. when a lyophilized mf and ao mixture was incubated at 40 degrees c and 65% relative humidity, the conjugation o ... | 2000 | 10637043 |
| urocortin is the principal ligand for the corticotrophin-releasing factor binding protein in the ovine brain with no evidence for a sauvagine-like peptide. | to purify novel ligands for the corticotrophin-releasing factor binding protein (crf-bp) from ovine brain, whole brain was homogenised in methanol and the supernatant extracted on sep-pak c18 cartridges followed by a preliminary hplc step. three peaks of ovine crf-bp ligand activity were detected in the hplc fractions, the first two of which were also detected by a specific corticotrophin-releasing factor two-site immunoradiometric assay, the third peak being detected by a human crf-bp ligand as ... | 2000 | 10656997 |
| use of green fluorescent protein (gfp) to study the invasion pathways of edwardsiella tarda in in vivo and in vitro fish models. | edwardsiella tarda is a fish pathogen that causes systemic infections in many food and ornamental fish. e. tarda ppd130/91 and ppd125/87 were selected as representatives of the virulent and avirulent groups, respectively, from eight fish isolates, and transformed with plasmids encoding either green fluorescent protein (pgfpuv) or blue fluorescent protein (pbfp2). two host models were used to study the invasion pathway of e. tarda in vitro and in vivo. epithelioma papillosum of carp (epc) was use ... | 2000 | 10658647 |
| biochemical characterization of different types of adherence of vibrio species to fish epithelial cells. | vibrio species are gram-negative bacteria that cause a systemic infection in fish called vibriosis. the authors previously demonstrated that internalization and cytotoxicity are important virulence mechanisms in vibrio-fish epithelial cell interactions. adherence is a prerequisite for successful internalization. in this study, the adherence capability of two invasive strains [v. anguillarum 811218-5w and g/virus/5(3)] was compared with that of two non-invasive strains [v. damselae atcc 33539 and ... | 2000 | 10784057 |
| recombinant extracellular domain of rabbit growth hormone receptor and biological activity of somatogenic hormones. | the cdna of the extracellular domain of rabbit growth hormone receptor (rbghr-ecd) was cloned in the prokaryotic expression vector pmon, to enable its expression in escherichia coli after induction with nalidixic acid. the bacterially expressed rbprlr-ecd protein, contained within the refractile-body pellet, was solubilized in 4.5 m urea, refolded, and purified on a q-sepharose column, ph 8, by stepwise elution with nacl. the bioactive monomeric 28-kda fraction was eluted in 0.15 m nacl, yieldin ... | 2000 | 10794181 |
| molecular cloning of a carp jak3 from activated leukocytes. | janus kinases (jaks) are involved in a signalling pathway leading to tyrosine phosphorylation, which is the key step in transducing cytokine signals from the external environment to the nucleus. we report here the molecular cloning of carp jak3. a partial cdna of the jak homologue was initially identified from a cdna pool obtained by subtracting the cdnas from con a-induced carp head kidney leukocytes with those from uninduced carp head kidney leukocytes. the entire coding sequence was assembled ... | 2000 | 10802290 |
| molecular and functional characterization of a fish inducible-type nitric oxide synthase. | using an oligonucleotide primer based on a partial goldfish inducible nitric oxide synthase (inos) sequence, a complete carp inos cdna was isolated from an activated carp phagocyte cdna library. nucleotide and predicted amino acid sequence analysis indicate that carp inos encodes a 1,127-amino acid protein with 57% sequence identity to human inos. like mammalian noss, carp inos protein contains putative binding sites for heme, tetrahydrobiopterin, calmodulin, flavine mononucleotide, flavine aden ... | 2000 | 10803847 |
| specific cell-mediated cytotoxicity against a virus-infected syngeneic cell line in isogeneic ginbuna crucian carp. | in the present study, we attempted to determine whether virus-specific cell-mediated cytotoxicity occurs in fish. experiments were conducted with clonal ginbuna crucian carp (carassius auratus langsdorfii) (s3n strain), and the syngeneic cell line (cfs). two infectious viruses were used: infectious pancreatic necrosis virus (ipnv) and eel virus from america (eva). peripheral blood leukocytes, which consisted almost entirely (95%) of lymphocytes and thrombocytes, from s3n ginbuna immunized with v ... | 2000 | 10831797 |
| purification of a novel serine proteinase inhibitor from the skeletal muscle of white croaker (argyrosomus argentatus). | a novel serine proteinase inhibitor has been purified to homogeneity from the skeletal muscle of white croaker (argyrosomus argentatus). the purification was carried out by ammonium sulfate fractionation, deae-sephacel, heating treatment followed by column chromatographies on sp-sepharose, sephadex g-150 and gel-filtration high performance liquid chromatography. the molecular mass of the inhibitor was 55 kda as estimated by sds-page and gel filtration. it specifically inhibited a myofibril-bound ... | 2000 | 10833440 |
| properties of cytotoxic peptide-formed ion channels. | cytotoxic peptides are relatively small cationic molecules such as those found 1) in venoms, e.g., melittin in bee, scorpion toxins in scorpion, pilosulin 1 in jumper ant, and lycotoxin i and ii in wolf spider; 2) in skin secretions (e.g., magainin i and ii from xenopus laevis, dermaseptin from frog, antimicrobials from carp) and cells of the immune system (e.g., insect, scorpion, and mammalian defensins and cryptdins); 3) as autocytotoxicity peptides, e.g., amylin cytotoxic to pancreatic beta-c ... | 2000 | 10837335 |
| cytopathological observations on renal tubule epithelium cells in common carp cyprinus carpio under trypanoplasma borreli (protozoa: kinetoplastida) infection. | cytological alterations in renal tubule epithelium cells of carp cyprinus carpio infected with the blood flagellate trypanoplasma borreli laveran & mesnil, 1901 were investigated during the course of a laboratory infection of a highly susceptible carp line. with the development of the parasitaemia, a hyperplasia of the interstitial renal tissue was induced, which resulted in a tubulus necrosis. cytological changes were already seen in tubulus epithelium cells on day 7 post injection (pi) of the ... | 2000 | 10843558 |
| near-ultraviolet magnetic circular dichroism spectroscopy of protein conformational states: correlation of tryptophan band position and intensity with hemoglobin allostery. | the near-uv magnetic circular dichroism spectroscopy of the aromatic amino acid bands of hemoglobin was investigated as a potential probe of structural changes at the alpha(1)beta(2) interface during the allosteric transition. allosteric effectors were used to direct carp and chemically modified human hemoglobins into the r (relaxed) or t (tense) state in order to determine the heme-ligation-independent spectral characteristics of the quaternary states. the tryptophan magnetic circular dichroism ... | 2000 | 10852712 |
| 1h spin-spin relaxation in normal and cataractous human, normal fish and bird eye lenses. | a systematic study on nuclear spin-spin relaxation of water protons in human, fish and bird eye lenses/lens nuclei is reported. the purpose of this study is to clarify the real nature of the relaxation processes not describable as a single exponential decay. the characterization of the spin-spin relaxation by a single exponential is commonly used both in literature and in mri diagnostics. however, in our opinion, this single exponential decay hypothesis is an oversimplification that can lead to ... | 2000 | 10866001 |
| differential scanning calorimetry of light meromyosin fragments having various lengths of carp fast skeletal muscle isoforms. | various recombinant light meromyosin (lmm) fragments were prepared from cdnas encoding the 10 degrees c and 30 degrees c types of myosin heavy chain isoforms predominantly expressed in fast skeletal muscles of the 10 degrees c- and 30 degrees c-acclimated carp, respectively. these included three kinds of quarter fragments, 1/4-, 2/4-, and 4/4-quarter, composed of residues 1-130, 131-270, and 401-563 from the n-terminus, respectively, as well as three halves, n-, m-, and c-half fragments, contain ... | 2000 | 10876153 |
| identification of a nuclear export signal in mkk6, an activator of the carp p38 mitogen-activated protein kinases. | carp homologues of p38 mitogen-activated protein kinase (mapk) and its activator mapk kinase 6 (mapkk6, referred to as mkk6) were identified. there exist at least two distinct carp p38s, cp38a and cp38b, both of which consist of 361 amino acids. the transcript of c38a was exclusively expressed in the ovary, whereas that of cp38b was ubiquitously expressed. western blot analysis with anti-(phosphorylated mapk) ig specific to the active p38 or jnk has shown that p38 was activated in response to hy ... | 2000 | 10880959 |
| the distribution of the introduced tapeworm bothriocephalus acheilognathi in australian freshwater fishes. | native and exotic fishes were collected from 29 sites across coastal and inland new south wales, queensland and victoria, using a range of techniques, to infer the distribution of bothriocephalus acheilognathi (cestoda: pseudophyllidea) and the host species in which it occurs. the distribution of b. acheilognathi was determined by that of its principal host, carp, cyprinus carpio; it did not occur at sites where carp were not present. the parasite was recorded from all native fish species where ... | 2000 | 10881282 |
| dna vaccines encoding viral glycoproteins induce nonspecific immunity and mx protein synthesis in fish. | protective immunity by vaccination with plasmid dna encoding a viral glycoprotein (g) has long been assumed to result from the induction of a specific immune response. we report here that the initial protection may be due to the induction of alpha/beta interferon, with long-term protection due to a specific response to the encoded viral g. dna vaccines encoding the gs of three serologically unrelated fish rhabdoviruses were used to vaccinate rainbow trout against a lethal challenge with infectio ... | 2000 | 10888644 |
| common egg envelope antigens are limited to the animal class. | the antigens of the egg envelope (zona pellucida) in mammals are of special interest because of their possible involvement in immunoinfertility and as candidate targets for immunocontraception. conserved zona epitopes from divergent species may present a suitable source and an animal model for investigation of the above factors. we compared egg envelope antigens from 6 species of vertebrates belonging to 3 different classes in order to demonstrate the existence of shared antigens. egg envelopes ... | 2000 | 10898216 |
| doxorubicin represses carp gene transcription through the generation of oxidative stress in neonatal rat cardiac myocytes: possible role of serine/threonine kinase-dependent pathways. | doxorubicin (dox), an anthracyclin antineoplastic agent, causes dilated cardiomyopathy. carp has been identified as a nuclear protein whose mrna levels are exquisitely sensitive to dox. in this study we investigated the molecular mechanisms underlying the repression of carp expression by dox in cultured neonatal rat cardiac myocytes. dox (1 micromol/l)-mediated decrease in carp mrna levels was strongly correlated with bnp but not with anp mrna levels. hydrogen peroxide scavenger catalase (1 mg/m ... | 2000 | 10900167 |
| cardiac ankyrin repeat protein is a novel marker of cardiac hypertrophy: role of m-cat element within the promoter. | carp, a cardiac doxorubicin (adriamycin)-responsive protein, has been identified as a nuclear protein whose expression is downregulated in response to doxorubicin. in the present study, we tested the hypothesis that carp serves as a reliable genetic marker of cardiac hypertrophy in vivo and in vitro. carp expression was markedly increased in 3 distinct models of cardiac hypertrophy in rats: constriction of abdominal aorta, spontaneously hypertensive rats, and dahl salt-sensitive rats. in additio ... | 2000 | 10904011 |
| carassius rfamide, a novel fmrfa-related peptide, is produced within the retina and involved in retinal information processing in cyprinid fish. | carassius rfamide (c-rfa) is a novel peptide, isolated originally from the brain of the japanese crucian carp and sharing homologies with mammalian prolactin-releasing peptide (prrp). it has been demonstrated previously that c-rfa mrna is abundant in the proximal half (fundus) of the japanese crucian carp eye. in the present work, we localized c-rfa by immunohistochemistry mainly to perikarya, in the proximal half of the inner nuclear layer (amacrine cell layer). this distribution is different f ... | 2000 | 10904133 |
| monoclonal antibodies to plasma high density lipoprotein (hdl) of eel (anguilla japonica). | six week-old female mice (balb/c) injected intraperitonealy with 50 micrograms of eel high density lipoprotein (hdl) emulsified with equal volume of adjuvant three times every two weeks. three weeks after the third injection, hyperimmunized mice were boosted by injection of 100 micrograms of hdl. after 5 days, the best responding mouse to injected hdl was sacrificed, and spleen cells were fused with mouse myeloma cells (sp2/o-ag14), and hybridomas were cultured in a selection medium. monoclonal ... | 2000 | 10904860 |
| the inferior oilvary complex. | although many studies have established the connections of the inferior olivary complex, there have been relatively few studies on the morphology of this nuclear complex. on the base of the similar topographic relations and connections with the cerebellum, the inferior olivary complex is regarded as homologous in all vertebrates. we present comprehensive light microscopical qualitative and quantitative analysis of the inferior olivary complex in different representatives of submammilian and mammi ... | 2000 | 10925479 |
| carp interleukin-1 beta in the role of an immuno-adjuvant. | 2000 | 10938746 | |
| molecular cloning and expression analysis of the putative carp (cyprinus carpio) pre-b cell enhancing factor. | 2000 | 10938748 | |
| complete genomic organization and promoter analysis of the round-spotted pufferfish jak1, jak2, jak3, and tyk2 genes. | we have previously reported the isolation of the jak1 gene from the round-spotted pufferfish. in the present study, we cloned and characterized genomic sequences encoding pufferfish jak2, jak3, and tyk2, which are other members of jak family. to our knowledge, this is the first report to demonstrate the existence of four jak genes in fish. all pufferfish jak genes except jak1 are composed of 24 exons; jak1 has an additional exon. a comparison of the exon-intron organization of these genes reveal ... | 2000 | 10945233 |
| in vitro cultivation of trypanoplasma borreli (protozoa: kinetoplastida), a parasite from the blood of common carp cyprinus carpio. | an in vitro culture system was developed for trypanoplasma borreli, a pathogenic flagellate from the blood of european cyprinids. trypanoplasms multiplied rapidly in a mixture of hanks' balanced salt solution (hbss, 45%), l15 (22.5%), earle's minimum essential medium (mem, 22.5%) and 10% distilled water, which was supplemented with 5 to 10% heat-inactivated pooled carp serum. in medium supplemented with fetal bovine serum, multiplication of t. borreli seemed to be inhibited. cultures initiated w ... | 2000 | 10950181 |
| flow cytometric analysis of proliferative responses of carp cyprinus carpio peripheral blood leukocytes to mitogens and to the hemoflagellate trypanoplasma borreli. | the activation of carp peripheral blood leukocytes (pbl) was analysed radiometrically and by means of flow cytometry (fcm) in order to compare the results obtained with both methods. the qualitative and quantitative fcm analyses of cellular morphology and viability resulted in a further characterisation of proliferative responses of carp pbl to trypanoplasma borreli in vivo and in vitro. the lymphocyte population of pbl from t. borreli-infected carp exhibited a marked shift in forward scattered ... | 2000 | 10950182 |
| effects of bothriocephalus acheilognathi on the polarization response of pronephric leucocytes of carp, cyprinus carpio. | an in vitro assay was used to examine the effect of bothriocephalus acheilognathi yamaguti, 1934 (cestoda: pseudophyllidea) on the polarization response of pronephric leucocytes of carp, cyprinus carpio. leucocytes, isolated from naive, naturally-infected fish and carp injected intraperitoneally with cestode extracts, were exposed to parasite extracts (protein concentrations 0-10.0 microg ml-1), for up to 24 h in the presence or absence of carp serum. in general, polarization responses of the pr ... | 2000 | 10953226 |
| position-independent expression of transgenes in zebrafish. | the variability in expression patterns of transgenes, caused by the influence of neighboring chromatin, is called 'position effect'. border elements are dna sequences, which have the ability to alleviate position effects. the abilities of two types of border elements, scs/scs' from the d. melanogaster 87a7 heat shock locus and the a-element from the chicken lysozyme gene, to protect transgenes from position effects were quantified in developing zebrafish embryos. the transgenic construct used wa ... | 1999 | 10669943 |
| expression of human blood group antigens a and b in stomach cells of c. carpio, c. auratus, r. ridibunda and h. sapiens. | human blood group abh antigens are found not only on red blood cell membranes, but in many other cell types as well. their biological functions still remain unclear. the aim of the present study was to examine the cellular expression of these antigens in the stomach of representatives of different vertebrates--pisces and amphibia and to compare it with their expression found in man. the immunohistochemical technique applied was based on the biotinstreptavidin-peroxidase complex. monoclonal antib ... | 1999 | 10658361 |
| expression of salmon corticotropin-releasing hormone precursor gene in the preoptic nucleus in stressed rainbow trout. | the behavior of genes encoding the corticotropin-releasing hormone (crh) precursor in response to stress has not been extensively studied in teleosts. to clarify this problem, crh cdnas were isolated from a hypothalamic cdna library of sockeye salmon, oncorhynchus nerka, by screening with pcr products amplified from the hypothalamic mrna with primers deduced from the sequence of the sucker crh precursor. two types of pcr products with a high degree of sequence homology were identified (crh-i and ... | 1999 | 9882547 |
| xenobiotic-metabolizing enzymes in carp (cyprinus carpio) liver, spleen, and head kidney following experimental listeria monocytogenes infection. | infection of carp with listeria monocytogenes 4b resulted in decreased liver, spleen, and head kidney enzyme activities, involved in the metabolism of xenobiotics. after infection, cytochrome p-450 levels and ethoxyresorufin o-deethylase (erod) activity were decreased while conjugation enzymes remained unaffected. the maximum decrease for phase i enzymes occurred on d 3. this loss of monooxygenase levels and activity could not be directly correlated with an increase in the number of organisms, a ... | 1999 | 9973004 |
| sequence differences between alpha1c and alpha1s ca2+ channel subunits reveal structural determinants of a guarded and modulated benzothiazepine receptor. | the molecular basis of the ca2+ channel block by (+)-cis-diltiazem was studied in class a/l-type chimeras and mutant alpha1c-a ca2+ channels. chimeras consisted of either rabbit heart (alpha1c-a) or carp skeletal muscle (alpha1s) sequence in transmembrane segments iiis6, ivs6, and adjacent s5-s6 linkers. only chimeras containing sequences from alpha1c-a were efficiently blocked by (+)-cis-diltiazem, whereas the phenylalkylamine (-)-gallopamil efficiently blocked both constructs. carp skeletal mu ... | 1999 | 10037699 |
| comparison of zp3 protein sequences among vertebrate species: to obtain a consensus sequence for immunocontraception. | the deduced zp3 amino acid (aa) sequences of 13 vertebrate species namely mouse, hamster, rabbit, pig, porcine, cow, dog, cat, human, bonnet, marmoset, carp, and frog were compared using the pileup and pretty alignment programs (gcg, wisconsin, usa). the published aa sequences obtained from 13 vertebrate species indicated the overall evolutionarily conservation in the n-terminus, central region, and c-terminus of the zp3 polypeptide. more variations of zp3 polypeptide sequences were seen in the ... | 1999 | 10051457 |
| whirling disease: host specificity and interaction between the actinosporean stage of myxobolus cerebralis and rainbow trout oncorhynchus mykiss. | scanning electron microscopic studies were conducted on rainbow trout oncorhynchus mykiss in the first 60 min after their exposure to the triactinomyxon spores of myxobolus cerebralis. the results demonstrated that as early as 1 min post exposure the whole process, from the attachment of the triactinomyxon spores to the complete penetration of their sporoplasm germs, had occurred. the triactinomyxon spores sought out the secretory openings of mucous cells of the epidermis, the respiratory epithe ... | 1999 | 10073310 |
| [detection of viral hemorrhagic septicemia virus (vhsv) in rainbow trout (oncorhynchus mykiss) by reverse transcription followed by polymerase chain reaction. diagnostic validation]. | viral haemorrhagic septicaemia virus (vhsv) is a fish pathogen that attacks rainbow trout (oncorhynchus mykiss) farming. the only diagnostic method recognised by the european community for vhs is based on the detection of the viral agent in cell culture followed by an immunological identification of the pathogen. reverse transcription followed by a double amplification of the polymerase chain reaction (rt-pcr) for the gene encoding the viral glycoprotein g is proposed here as an alternative meth ... | 1999 | 10081112 |
| genotoxicity of drinking water from chao lake. | genotoxic activity appears to originate primarily from reactions of chlorine with humic substances in the source waters. comparisons of extracts of settled versus chlorinated water have confirmed that chlorinating during water treatment produces mutagenic activity in the mutagenicity tests. present work on xad-2 extracts of raw, chlorinated (treated), and settled water from the chao lake region of china has involved a battery of mutagenicity assays for various genetic endpoints: the salmonella t ... | 1999 | 10092404 |
| characterization of a cdna encoding a precursor of carassius rfamide, structurally related to a mammalian prolactin-releasing peptide. | we have characterized the cdna encoding carassius rfamide (c-rfa), which is structurally related to mammalian prolactin-releasing peptides (prrps), from the brain of the crucian carp. the deduced c-rfa precursor has been shown to comprise 117 amino acids, encoding a single c-rfa sequence. a comparative study of amino acid sequences has revealed that several sequences conserved in preproprrps are also found in the c-rfa precursor. furthermore, the abundant presence of the c-rfa mrna in the telenc ... | 1999 | 10100851 |
| induction of two major isoforms of metallothionein in crucian carp (carassius cuvieri) by air-pumping stress, dexamethasone, and metals. | the induction of metallothionein (mt) by physical and chemical stress was assessed using the fresh-water fish, crucian carp (carassius cuvieri temminck et schlegel). the fish exposed to violent air-pumping stress for 6 days revealed time-dependent induction of mt-like metal-binding proteins in both their livers and kidneys. their hepatic contents after exposure to stress were elevated to twice the basal level with 24 h, resulting in more than a 3-fold increase at 144 h, whereas their renal conte ... | 1999 | 10190030 |
| kainate-elicited seizures induce mrna encoding a camk-related peptide: a putative modulator of kinase activity in rat hippocampus. | by means of differential display techniques, we have previously identified an mrna transcript whose expression is highly induced in the rat hippocampus by kainate-elicited seizures. here, we report the cloning of a corresponding cdna encoding a 55-amino-acid, serine-rich peptide which contains four predicted phosphorylation sites. the peptide was designated camk-related peptide (carp) as it shares significant amino acid sequence identity with part of a novel putative calcium/calmodulin-dependent ... | 1999 | 10213452 |
| identification of serum gh-binding proteins in the goldfish (carassius auratus) and comparison with mammalian gh-binding proteins. | the present study constitutes the characterization of a specific, high-affinity gh-binding protein (ghbp) in the serum of a teleost, the goldfish (carassius auratus). gh-binding assay and ligand blotting techniques were employed to identify ghbps in goldfish serum and hepatocyte culture medium. the binding characteristics and apparent molecular weights (mr) of goldfish ghbps were also compared with those of rabbit and rat. ligand analysis identified a single class of high-affinity and low-capaci ... | 1999 | 10320823 |
| [trisomy 9p: a clinical picture and the importance of examining the family]. | in three patients, two males aged four months and 39 years, and a female aged 19 years, trisomy 9p (the threefold presence of the short arm of chromosome 9) was diagnosed. the main symptoms are mental retardation, brachycephaly, hypertelorism with deep-set eyes, broad base of the nose, short philtrum, carp-shaped mouth, cup-shaped ears, short fingers and clinodactyly. trisomy 9p is often caused by a balanced translocation in one of the parents. therefore, cytogenetic studies of the parents and i ... | 1999 | 10321302 |
| purification and characterization of insulin and peptides derived from proglucagon and prosomatostatin from the fruit-eating fish, the pacu piaractus mesopotamicus. | the fruit-eating teleost fish, the pacu piaractus mesopotamicus (characiformes, characidae) is classified along with the carp and the catfish in the superorder ostariophysi. the pacu is able to survive and grow in captive conditions feeding exclusively on carbohydrates. hormonal polypeptides in an extract of pacu brockmann bodies were purified to homogeneity by reversed phase hplc and their primary structures determined by automated edman degradation. pacu insulin contains only two substitutions ... | 1999 | 10327603 |
| glutathione depletion causes an uncoupling effect on retinal horizontal cells through oxidative stress. | to investigate a physiological role of glutathione in the horizontal cells of carp retina, the gap junctional intercellular communication between horizontal cells was studied using the techniques of intracellular recording of light-induced responses and coupling of the fluorescence dye lucifer yellow. intravitreal injection of 2.5 micromol l-buthionine sulfoximine, an inhibitor of glutathione synthesis, induced a dramatic reduction (20% of control) of retinal glutathione level two days after tre ... | 1999 | 10338315 |
| yersinia ruckeri septicaemia in experimentally infected carp (cyprinus carpio l.) fingerlings. | the presence of yersinia ruckeri, the causal agent of enteric redmouth disease (erm) in salmonids and a few other freshwater fish, has so far been reported from a variety of sources including the intestine of healthy carp. since there are no data on the pathogenicity of this bacterium for carp, 15 fingerlings were experimentally infected by intraperitoneal injection of about 5 x 10(5) cells. thirteen injected fish were moribund or died within 4 days with septicaemic lesions. two survivors were s ... | 1999 | 10344077 |
| herpesvirus-associated papillomas in koi carp (cyprinus carpio). | from january through november 1994, 32% (7/22) of koi carp (cyprinus carpio) maintained in indoor aquariums developed proliferative cutaneous lesions that consisted of single to multiple 2-10-mm whitish to pink fleshy masses usually associated with fin rays. although scaleless koi were more commonly affected (3/6) than were normally scaled koi (4/16), the difference in incidence rates was not significant (chi2 text, p > 0.05). lesions typically resolved spontaneously in 1-3 wk, occasionally pers ... | 1999 | 10367660 |
| purification and characterization of alpha1-proteinase inhibitor from carp (cyprinus carpio) serum. | : alpha1-proteinase inhibitor was purified to homogeneity from carp serum with an increase in specific inhibitory activity of 17-fold and a 3% recovery rate. the inhibitor was estimated to have molecular weight of 55,000 under reducing and nonreducing conditions, indicating its composition of a single polypeptide. the inhibitor immunologically crossreacted faintly with carp muscular serine proteinase inhibitor but had no crossreactivity with serine proteinase inhibitors from other species. carp ... | 1999 | 10373608 |
| purification and characterization of serum serpin from carp (cyprinus carpio). | : a serine proteinase inhibitor, termed serpin62, was purified to homogeneity from carp serum with an increase in specific inhibitory activity of 6.2-fold and a 3% recovery rate after separation from alpha1-antitrypsin. specific inhibitory activity of serpin62 against bovine pancreatic trypsin was less than half of the specific antitryptic activity of alpha1-antitrypsin. under both reducing and nonreducing conditions, serpin62 was estimated to have a molecular weight (62,000) apparently larger t ... | 1999 | 10373614 |
| ichthyophthiriasis in carp cyprinus carpio: infectivity of trophonts prematurely exiting both the immune and non-immune host. | ichthyophthirius multifiliis exposed to naturally immunised carp established short-term infections, the majority of parasites actively emerging within 2 h of entering the epidermis. a small, but significant, number of these expelled parasites were shown to retain theront-like properties with the capacity to directly re-invade a further fish host. infectivity fell rapidly with time in the host and was comparable to that of trophonts of a similar age artificially induced to emerge from non-immune ... | 1999 | 10401585 |
| effects of thermal acclimation on the relaxation system of crucian carp white myotomal muscle. | many cyprinid fish are able to compensate for a decrease in ambient temperature by process of physiological adaptation in the function of muscles. in the winter habitat of crucian carp (carassius carassius l.), low temperature is associated with simultaneous oxygen shortage. because of the oxygen deprivation, there is probably little space for compensatory adaptation because positive thermal compensation would increase energy demand and accelerate depletion of glycogen reserves. thus, we assumed ... | 1999 | 10404115 |
| antibody response of carp, cyprinus carpio to the cestode, bothriocephalus acheilognathi. | the humoral antibody response and the number of pronephric antibody-secreting cells were examined in naturally bothriocephalus acheilognathi-infected carp. cyprinus carpio, and in those injected intraperitoneally with an extract of the cestode. in the extract-injected fish, specific antibody was detected 3 weeks after a second injection given 2 weeks after the primary injection, and antibody levels persisted for more than 200 days. a third injection also enhanced the antibody level in the extrac ... | 1999 | 10406042 |