Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| bacillus cereus meningitis complicating cerebrospinal fluid fistula repair and spinal drainage. | non-anthrax bacillus species are rare, but serious causes of bacterial meningitis in those either immunocompromised or treated with csf diversion. although resistant to first-line antibiotics, they usually respond to chloramphenicol. we report a case of fulminant bacillus cereus meningitis that complicated lumbar spinal drainage which proved resistant to all first-line antibiotics including chloramphenicol. | 2000 | 11272043 |
| bioactivity studies of extracts from tridax procumbens. | an updated review on the biological activity of tridax procumbens is presented. a detailed biological screening comprised of gram-positive and gram-negative bacteria, yeasts and fungi using crude extracts of this plant was undertaken. the n-hexane extract of the flowers showed activity against escherichia coli. the same extract of the whole aerial parts was active against mycobacterium smegmatis, escherichia coli, salmonella group c and salmonella paratyphi. the ethyl-acetate extract of the flow ... | 2000 | 11185735 |
| [study of the virulence of a recombinant strain of bacillus anthracis, obtained as a result of transductive transfer of chromosomal genes from a strain of bacillus cereus]. | auxotrophic markers of b. anthracis strains differing them from other bacillus representatives have been determined. chromosome genes from prototrophic b. cereus strain were transduced into auxotrophic b. anthracis strain. the properties of transductants were studied in order to establish common transfer of chromosomal determinants responsible for realization of various signs. transduction mating between species resulted in construction of prototroph b. anthracis strains (px01- px02+), whose der ... | 2000 | 11186451 |
| [effect of increased pressure and changed gaseous media on biological properties of microorganisms]. | bacterial contamination of diving outfit and inner surfaces of pressure complex increases at the expense of expansion of gram-negative microflora during long exploitation of undersea complexes. gram-negative bacteria were hypothesized to effectively adapt to hyperbaric conditions. we investigated the effect of hyperbaric conditions and changed gaseous environment on the cultural and morphological characteristics of colonies, growth rate and time of generation of test cultures (p. aeruginosa atcc ... | 2000 | 11186454 |
| synthesis and antimicrobial activity of new adamantane derivatives ii. | a series of new derivatives of adamantane was synthesised. the new compound 4-(adamant-1-ylethylenoxycarbonyl)phthalanhydride obtained from 1-adamantaneethanol and trimellitic anhydride chloride, as well as 4-(adamant-1-ylmethylenoxycarbonyl)phthalanhydride, appeared very useful for preparation of a number of n-substituted phthalimides. antimicrobial activity of newly obtained derivatives such as, for example, 4-(adamant-1-ylethylenoxycarbonyl)-n-(l-phenylalanyl)phthalimide or 4-(adamant-1-ylmet ... | 2000 | 11152243 |
| synthesis and biological studies of some benzopyran. | 3-chlorobenzopyrano[2,3-c]pyrazole (2) was prepared and reacted with sodium azide to give compound 3, whereas its reaction with benzoyl hydrazide, ethyl glycinate, anthranilic acid or o-phenylenediamine afforded the products 4-7, respectively. compounds 8 and 9 were synthesized by the reaction of compound 2 with 2-mercaptobenzothiazole or piperidine. 3-hydrazinobenzopyrano[2,3-c]pyrazole (10) was obtained and subjected to cyclization with different reagents such as cs2, benzoic acid, acetyl acet ... | 2000 | 11152247 |
| an active-site mutation causes enhanced reactivity and altered regiospecificity of transglucosylation catalyzed by the bacillus sp. sam1606 alpha-glucosidase. | bacillus sp. sam1606 alpha-glucosidase catalyzes the transglucosylation of sucrose to produce three regioisomers of the glucosylsucroses, with theanderose (6-o(g)-glucosylsucrose) as the most abundant transfer product. to find the active-site amino acid residues which can affect the reactivity and regiospecificity of the glucosyl transfer, 16 mutants with amino acid substitutions near the active site were allowed to react with 1.75 m sucrose at 60 degrees c, ph 6.0, and the course of transglucos ... | 2000 | 16232773 |
| a case of bovine mastitis caused by bacillus cereus. | 1999 | 16032094 | |
| a comparison of bacillus cereus and aeromonas hydrophilia zn-beta-lactamases. | 1999 | 15263322 | |
| arthromitus (bacillus cereus) symbionts in the cockroach blaberus giganteus: dietary influences on bacterial development and population density. | the filamentous spore-forming bacterium arthromitus, discovered in termites, millipedes, sow bugs and other soil-dwelling arthropods by leidy (1850), is the intestinal stage of bacillus cereus. we extend the range of arthromitus habitats to include the hindgut of blaberus giganteus, the large tropical american cockroach. the occurrence and morphology of the intestinal form of the bacillus were compared in individual cockroaches (n=24) placed on four different diet regimes: diurnally maintained i ... | 1999 | 11762374 |
| climatic influence on mesophilic bacillus cereus and psychrotolerant bacillus weihenstephanensis populations in tropical, temperate and alpine soil. | bacillus weihenstephanensis strains are psychrotolerant and grow from below 7 degrees c to 38 degrees c. closely related mesophilic bacillus cereus strains can grow from above 7 degrees c to 46 degrees c. we classified 1060 b. cereus group isolates from different soil samples with respect to their psychrotolerant and mesophilic genotypes by polymerase chain reaction (pcr) targeting of specific 16s rdna and cold shock protein a gene signatures. in parallel, growth tests at 7 degrees c were carrie ... | 1999 | 11207772 |
| [studies on the parasites of paris polyphylla var. yunnanensis]. | two bacteria and three fungi were isolated from the hypogeal stems of paris polyphylla var. yunnanensis. the bacteria were identified as bacillus cereus and pseudomonas alcaligenes, and the fungi were identified as periconia sp., pachnocybe albida and hormomyces paridiphilus. the results on liquid culture of b. cereus, p. alcaligenes and h. paridiphilus indicated that the colloidization and polysaccharide content increasing in hypogeal stems of p. polyphylla var. yunnanensis were due to the extr ... | 1999 | 12555422 |
| [purification and characterization of a novel antifungal peptide aps-1 produced by bacillus cereus]. | in previous study, we isolated an antagonist bacillus cereus strain: s-1 from cotton plant. in field experiments, this bacterium was shown strong inhibition to several plant diseases. in this paper, we reported the purification of the antifungal substance produced by the bacterium and its properties. after the steps of acid precipitation, methanol and ethy lether extraction, sephadex g100 and deae52 column chromatography, the antifungal material was purified. the purified material had absorption ... | 1999 | 12555574 |
| fluorimetric determination of ampicillin by use of non-toxic catalysts. estimation of beta-lactamase activity and parameters. | a fluorescence assay based on the use of biological reducing agents as catalysts rather than heavy metal ions has been developed for estimation of ampicillin concentrations. the assay is based on the conversion of ampicillin to its penicilloate, by treatment with sodium hydroxide, then neutralization, dilution with 0.5 m acetate buffer at ph4 and heating for 30 min at 100 degrees c in the presence of ascorbic acid (0.5 mg) and edta (50 microm). reduced glutathione, cysteine and n-acetylcysteine ... | 1999 | 10678504 |
| the structure of two fengycins from bacillus subtilis s499. | the structures of the two fengycins, lipopeptides from bacillus subtilis, were elucidated by spectroscopic methods and chemical degradation. they show a close structural relationship to the plipastatins from bacillus cereus differing only in the stereochemistry of the tyr residues. | 1999 | 10660307 |
| clinical manifestations of bacillus cereus meningitis in newborn infants. | bacillus cereus (b. cereus) meningitis sometimes occurs in patients with risk factors, which are associated with central nervous system (cns) anomalies, surgical or anaesthetic access to cns. we observed two cases of b. cereus meningitis in neonates without such risk factors. the clinical courses of both neonates were fulminant, and routine antibiotic therapy failed. intracranial haemorrhage was evident at autopsy. according to the previous neonatal case reports and our experience, we found that ... | 1999 | 10620178 |
| antibacterial activity of orobanche cernua extract. | the effect of the parasitic plant orobanche cernua extract was evaluated on 5 different gram-positive and gram-negative bacteria. dilution experiments show that the minimum inhibitory concentration (mic; microgram/ml) of the extract was as follows: staphylococcus aureus-1526.5; streptococcus sp.-3125; streptococcus pyogenes-25,000 and both for bacillus cereus and escherichia coli-50,000 micrograms/ml. data indicated that the mic of 3125 micrograms/ml is equal to cefotaxime (30 micrograms) and to ... | 1999 | 10629975 |
| antibacterial hydroxycinnamic esters from piper caninum from paluma, north queensland, australia. the crystal and molecular structure of (+)-bornyl coumarate. | the crude chloroform bark extract of piper caninum (piperaceae) exhibits antibacterial activity against the gram-positive bacteria, bacillus cereus, staphylococcus aureus, and streptococcus pneumoniae. the antibacterial agents in this extract have been isolated using bioactivity-directed chromatographic techniques and identified by nmr spectroscopy as (+)-bornyl p-coumarate and bornyl caffeate. a single-crystal x-ray structure has been carried out on (+)-bornyl p-coumarate. the compound crystall ... | 1999 | 10630119 |
| occurrence of nisin z production in lactococcus lactis bfe 1500 isolated from wara, a traditional nigerian cheese product. | screening for bacteriocin production of 500 strains of lactic acid bacteria (lab) from various african fermented foods resulted in the detection of a bacteriocin producing lactococcus lactis (bfe 1500) isolated from a dairy product called wara. the bacteriocin inhibited not only the closely related lab, but also strains of listeria monocytogenes, listeria innocua, clostridium butyricum, clostridium perfringens, bacillis cereus and staphylococcus aureus. it was heat stable even at autoclaving tem ... | 1999 | 10634705 |
| heterogeneity observed in the components of hemolysin bl, an enterotoxin produced by bacillus cereus. | hemolysin bl (hbl), a diarrheal enterotoxin originally isolated from bacillus cereus strain f837/76, is composed of three antigenically distinct proteins designated b, l1, and l2. all three components are required for biological activity. here, we report antigenic and physical variations in hbl components produced by other b. cereus isolates. reactions of partial identity were observed in double gel immunodiffusion assays using antibodies to highly purified b, l1, and l2 components of f837/76 an ... | 1999 | 10634707 |
| [the monosaccharide composition of the glycocalyx in mollicutes and phylogenetically related bacteria in the genus bacillus]. | a number of monosaccharides composing the glycocalix carbohydrates of some mollicutes (mycoplasma pneumoniae fh, m. hominis pg21, m. fermentans pg18, acholeplasma laidlawii pg8, a. laidlawii var. granulum st. 118) and phylogenetically related bacteria from the genus bacillus (bacillus cereus 89, b. cereus dm423, b. subtilis 1/2, b. licheniformis 31, b. licheniformis 49) were detected using plant lectins of definite carbohydrate specificity labelled by colloid gold. the structure of extracellular ... | 1999 | 10643272 |
| an outbreak of bacillus cereus respiratory tract infections on a neonatal unit due to contaminated ventilator circuits. | an outbreak of bacillus cereus respiratory tract infections affecting six ventilated preterm neonates over a two-week period is described. reusable ventilator circuits were identified as the cause of the outbreak. ordinarily these were reprocessed on the neonatal unit (nnu), first through a washing machine and then through a low-temperature steam (lts) disinfector. the onset of the outbreak coincided with a breakdown of the lts facility, which necessitated sending the washed circuits off site fo ... | 1999 | 9949960 |
| fulminant septicaemic syndrome of bacillus cereus: three case reports. | three patients with acute leukaemia, who were severely neutropenic and iatrogenically immunosuppressed post-chemotherapy, developed rapidly fatal septicaemic shock and coma caused by bacillus cereus (b. cereus). the illness was marked by two phases: a mild febrile illness lasting 6-14 h and accompanied by subtle symptoms of autonomic sympathetic nervous system overactivity, and a second short fulminant one, marked by high fever of 40-41 degrees c accompanied by major central nervous system distu ... | 1999 | 10609535 |
| the in vitro antibacterial activity of turkish medicinal plants. | a total of 76 extracts from 35 plants available in the turkish flora were assayed for their in vitro antibacterial activities against five pathogenic bacteria and a yeast. sixteen crude extracts from eight plant species were found to possess an activity against at least one or more test microorganisms. bioassay-guided fractionation of the most active crude extracts was also carried out with the most active extracts. activity against staphylococcus aureus, bacillus cereus, branhamella catarrhalis ... | 1999 | 10616963 |
| bacillus cereus prosthetic valve endocarditis. | bacillus cereus is a ubiquitous organism that often contaminates microbiological cultures but rarely causes serious infections. reports of b. cereus endocarditis are infrequent. infection in patients with valvular heart disease is associated with significant mortality and morbidity. we describe a case of b. cereus endocarditis involving a mechanical mitral prosthesis that resolved after replacement of the prosthetic valve. we also review the previous cases reported in the literature. | 1999 | 10617040 |
| microbiological quality and safety of ready-to-eat street-vended foods in johannesburg, south africa. | fifty-one ready-to-eat street foods, 18 dishwater, and 18 surface swab samples were collected from six vendors in johannesburg, south africa. food temperatures were recorded at the time of sampling. standard methods were used to determine aerobic plate counts (apcs), spore counts (scs), and enterobacteriaceae counts (ecs) for food samples as well as coliform counts (ccs) for water and swab samples. in addition, petrifilm escherichia coli count (pc) plates were used for the enumeration of colifor ... | 1999 | 10571317 |
| rt-pcr: characterization of long multi-gene operons and multiple transcript gene clusters in bacteria. | reverse transcription (rt)-pcr is a valuable tool widely used for analysis of gene expression. in bacteria, rt-pcr is helpful beyond standard protocols of northern blot rna/dna hybridization (to identify transcripts) and primer extension (to locate their start points), as these methods have been difficult with transcripts that are low in abundance or unstable, similar to long multi-gene operons. in this report, rt-pcr is adapted to analyze transcripts that form long multi-gene operons--where the ... | 1999 | 10572645 |
| distribution and subcellular localization of acylpeptide hydrolase and acylase i along the hog gastro-intestinal tract. | the distribution of acylase i and acylpeptide hydrolase along the hog small intestine was investigated. no significant changes in their respective specific activity was found when the intestine was cut off and divided into eight segments (taken every 200 cm) so as to specifically study the duodenum, jejunum and ileum. upon performing subcellular fractionation of hog enterocytes, it was observed that acylpeptide hydrolase is a soluble enzyme, while acylase i is essentially a soluble protein accou ... | 1999 | 10575361 |
| enterotoxigenic profiles and polymerase chain reaction detection of bacillus cereus group cells and b. cereus strains from foods and food-borne outbreaks. | bacillus cereus is one of the important food pathogens. since b. cereus group cells, such as b. cereus, b. thuringiensis, b. anthracis and b. mycoides, share many phenotypical properties and a high level of chromosomal sequence similarity, it is interesting to investigate the virulence profiles for b. cereus group cells, including b. cereus strains isolated from foods and samples associated with food-poisoning outbreaks. for this investigation, the presence of enterotoxin genes, such as those of ... | 1999 | 10583675 |
| the hemolytic enterotoxin hbl is broadly distributed among species of the bacillus cereus group. | the prevalence of the hemolytic enterotoxin complex hbl was determined in all species of the bacillus cereus group with the exception of bacillus anthracis. hbla, encoding the binding subunit b, was detected by pcr and southern analysis and was confirmed by partial sequencing of 18 strains. the sequences formed two clusters, one including b. cereus and bacillus thuringiensis strains and the other one consisting of bacillus mycoides, bacillus pseudomycoides, and bacillus weihenstephanensis strain ... | 1999 | 10584001 |
| bacillus cereus infections among oncology patients at a children's hospital. | bacillus cereus can cause severe infections in immunocompromised persons. | 1999 | 10586160 |
| sequence analysis of three bacillus cereus loci carrying picr-regulated genes encoding degradative enzymes and enterotoxin. | picr is a pleiotropic regulator of extracellular virulence factors in the opportunistic human pathogen bacillus cereus and the entomopathogenic bacillus thuringiensis, and is induced in cells entering stationary phase. among the genes regulated by picr are: pica, encoding phosphatidylinositol-specific phospholipase c (pi-plc); plc, encoding phosphatidylcholine-preferring phospholipase c (pc-plc); nhe, encoding the non-haemolytic enterotoxin; hbl, encoding haemolytic enterotoxin bl (hbl); and gen ... | 1999 | 10589720 |
| insertional inactivation of hblc encoding the l2 component of bacillus cereus atcc 14579 haemolysin bl strongly reduces enterotoxigenic activity, but not the haemolytic activity against human erythrocytes. | haemolysin bl (hbl) is a bacillus cereus toxin composed of a binding component, b, and two lytic components, l1 and l2. hbl is also the enterotoxin responsible for the diarrhoeal food poisoning syndrome caused by several strains of b. cereus. the three genes encoding the hbl components constitute an operon and are transcribed from a promoter 608 bp upstream of the hblc translational start site. the first gene of the hbl operon, hblc, in the b. cereus type strain, atcc 14579, was inactivated in t ... | 1999 | 10589721 |
| thermal inactivation of bacillus cereus spores affected by the solutes used to control water activity of the heating medium. | the heat resistance of b. cereus spores (atcc 7004, 4342 and 9818) over a wide temperature range (92-125 degrees c) in aqueous solutions of nacl, licl, sucrose and glycerol at different water activities (1.00-0.71) was investigated. sodium chloride in the heating medium tended to protect the spores of b. cereus against heat. the z-values increased significantly (p < 0.05) at and above a concentration of 4.0 m. the effects of licl were lower than those caused by the nacl at the same a(w) values. ... | 1999 | 10598115 |
| aqueous and vitreous penetration of levofloxacin after oral administration. | to investigate the penetration of levofloxacin, an optical s-(-)isomer of ofloxacin, into the aqueous and vitreous humor after oral administration. | 1999 | 10599658 |
| preference of cd(ii) and zn(ii) for the two metal sites in bacillus cereus beta-lactamase ii: a perturbed angular correlation of gamma-rays spectroscopic study. | cd-substituted forms of the bacillus cereus metallo-beta-lactamases (bcii) were studied by perturbed angular correlation of gamma-rays (pac) spectroscopy. at very low [cd]:[apo-beta-lactamase] ratios, two nuclear quadrupole interactions (nqi) were detected. for [cd]:[apo-beta-lactamase] ratios between 0.8 and 3.0, two new nqis appear, and the spectra show that up to 2 cadmium ions can be bound per molecule of apoenzyme. these results show the existence of two interacting cd-binding sites in bcii ... | 1999 | 10600111 |
| growth conditions of and emetic toxin production by bacillus cereus in a defined medium with amino acids. | the growth and emetic toxin (cereulide) production of bacillus cereus strains in defined culture media were studied. we found that a fully synthetic medium (cadm) allowed the production of emetic toxin and the addition of glucose enhanced it. by subtracting each amino acid from cadm, we found that only three amino acids, valine, leucine and threonine, were essential for growth and toxin production by b. cereus. the addition of high levels (50 mm) of leucine, isoleucine and glutamic acid decrease ... | 1999 | 10100742 |
| cloning and nucleotide sequence analysis of gyrb of bacillus cereus, b. thuringiensis, b. mycoides, and b. anthracis and their application to the detection of b. cereus in rice. | as 16s rrna sequence analysis has proven inadequate for the differentiation of bacillus cereus from closely related species, we employed the gyrase b gene (gyrb) as a molecular diagnostic marker. the gyrb genes of b. cereus jcm 2152(t), bacillus thuringiensis iam 12077(t), bacillus mycoides atcc 6462(t), and bacillus anthracis pasteur #2h were cloned and sequenced. oligonucleotide pcr primer sets were designed from within gyrb sequences of the respective bacteria for the specific amplification a ... | 1999 | 10103241 |
| semiautomated metabolic staining assay for bacillus cereus emetic toxin. | this paper describes a specific, sensitive, semiautomated, and quantitative hep-2 cell culture-based 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay for bacillus cereus emetic toxin. of nine bacillus, brevibacillus, and paenibacillus species assessed for emetic toxin production, only b. cereus was cytotoxic. | 1999 | 10103289 |
| evaluation of methods for recognising strains of the bacillus cereus group with food poisoning potential among industrial and environmental contaminants. | toxin production, biochemical properties and ribotypes of bacillus cereus group (b. cereus, b. thuringiensis, b. mycoides) strains originating from industrial and environmental sources (n = 64), from food poisoning incidents (n = 22) and from reference sources (n = 7) were analysed. forty ribotypes were found among the 93 strains. eleven strains from food poisoning incidents produced emetic (mitochondrio) toxin, as determined by the boar spermatozoa toxicity test. these strains possessed closely ... | 1999 | 10188285 |
| uptake kinetics of nucleic acid targeting dyes in s. aureus, e. faecalis and b. cereus: a flow cytometric study. | for flow cytometry-based detection as well as susceptibility testing and counting, staining of the bacterial cells is essential. in an attempt to develop rapid preparatory procedures for nucleic acid staining of wild type gram positive bacteria, the uptake of fluorescent dyes in viable s. aureus, e. faecalis, and b. cereus cells was studied by flow cytometry under conditions intended to block probe efflux and increase cell wall permeability. the aim of the study was to develop procedures which a ... | 1999 | 10192050 |
| catalytic cycle of the phosphatidylcholine-preferring phospholipase c from bacillus cereus. solvent viscosity, deuterium isotope effects, and proton inventory studies. | the phosphatidylcholine-preferring phospholipase c from bacillus cereus (plcbc) is a 28.5 kda enzyme with three zinc ions in its active site. although much is known about the roles that various plcbc active site amino acids play in binding and catalysis, there is little information about the rate-determining step of the plcbc-catalyzed hydrolysis of phospholipids and the catalytic cycle of the enzyme. to gain insight into these aspects of the hydrolysis, solvent viscosity variation experiments w ... | 1999 | 10194360 |
| detection of toxigenic strains of bacillus cereus and other bacillus spp. with an improved cytotoxicity assay. | an improved qualitative cell cytotoxicity assay for the detection of bacillus cereus emetic and enterotoxin is described. the presence of toxin in culture supernatant fluids was detected by measurement with the tetrazolium salt mtt, as it adversely affects the metabolic status of cultured cho cells. psychrotrophic b. cereus isolates (65) were assessed for toxin production using the cytotoxicity assay, and 91% of culture supernatant fluids were cytotoxic. toxin assessment using bcet-rpla and elis ... | 1999 | 10196773 |
| expression of a germination-specific amidase, sleb, of bacilli in the forespore compartment of sporulating cells and its localization on the exterior side of the cortex in dormant spores. | a germination-specific amidase of bacilli is a major spore-lytic enzyme that is synthesized with a putative signal sequence and hydrolyses spore cortex in situ. the sleb gene encoding this amidase in bacillus subtilis and bacillus cereus was expressed in the forespore compartment of sporulating cells under the control of sigmag, as shown by northern blot and primer extension analyses. the forespore-specific expression of b. subtilis sleb was further indicated by the forespore-specific accumulati ... | 1999 | 10197998 |
| correlation of 16s ribosomal dna signature sequences with temperature-dependent growth rates of mesophilic and psychrotolerant strains of the bacillus cereus group. | sequences of the 16s ribosomal dna (rdna) from psychrotolerant and mesophilic strains of the bacillus cereus group revealed signatures which were specific for these two thermal groups of bacteria. further analysis of the genomic dna from a wide range of food and soil isolates showed that b. cereus group strains have between 6 and 10 copies of 16s rdna. moreover, a number of these environmental strains have both rdna operons with psychrotolerant signatures and rdna operons with mesophilic signatu ... | 1999 | 10198030 |
| the role of the non-conserved residue at position 104 of class a beta-lactamases in susceptibility to mechanism-based inhibitors. | the role of the non-conserved amino acid residue at position 104 of the class a beta-lactamases, which comprises a highly conserved sequence of amino acids at the active sites of these enzymes, in both the hydrolysis of beta-lactam substrates and inactivation by mechanism-based inhibitors was investigated. site-directed mutagenesis was performed on the penpc gene encoding the bacillus cereus 569/h beta-lactamase i to replace asp104 with the corresponding staphylococcus aureus pc1 residue ala104. ... | 1999 | 10209286 |
| dust-borne bacteria in animal sheds, schools and children's day care centres. | a total of 316 bacterial strains, including psychrophiles, mesophiles and thermophiles, were isolated and identified from indoor dusts in schools, children's day care centres and animal sheds. several species which had not previously been reported from indoor environments were found: sphingomonas, brevibacterium, nocardiopsis, deinococcus and rhodococcus/gordona. a new psychrophilic actinomycete genus was also found in animal sheds, representing a new undescribed peptidoglycan type and an unusua ... | 1999 | 10212408 |
| preparation, 195pt nmr spectra and biological activity of platinum (iv) complexes with dipeptides. | three dipeptide complexes of the form k[pt(iv) (dipep) cl(oh)2] and four dipeptide complexes of the form k[pt(iv)-(hdipep)cl2(oh)2] were newly prepared. the 195 pt nmr peak of the k[pt(iv) (dipep)cl(oh)2] complexes appeared at about 1200 ppm and these chemical shifts were about 3150 ppm downfield compared with those of the k[pt(ii) (dipep) cl] complexes. the chemical shifts of the k[pt(iv) (hdipep) cl2 (oh)2] complexes were at about 900 ppm, i.e., about 3050 ppm downfield compared with those of ... | 1999 | 10212991 |
| innate immunity in insects: the role of multiple, endogenous serum lectins in the recognition of foreign invaders in the cockroach, blaberus discoidalis. | unlike vertebrates, insects do not have an ab-based nonself recognition system, and must rely totally on innate immunity to defend themselves from microbial invaders. the most likely candidates for recognizing foreign material in insects are the lectins, which have already been shown to be important in mammalian innate immunity. the hemolymph of the cockroach, blaberus discoidalis, contains multiple lectins, designated bdl1, bdl2, bdl3, and gsl (beta-1,3-glucan-specific lectin), two of which, na ... | 1999 | 9973417 |
| biocontrol of plant disease: a (gram-) positive perspective. | biological control offers an environmentally friendly alternative to the use of pesticides for controlling plant diseases. unfortunately, growers continue to use chemical control over biological agents, and lack of knowledge often contributes to the downfall of a biocontrol agent. knowledge of the biological environment in which the agent will be used and of how to produce a stable formulation are both critical to successful biocontrol. certain gram-positive bacteria have a natural formulation a ... | 1999 | 9987836 |
| suitability of the charm hvs and a microbiological multiplate system for detection of residues in raw milk at eu maximum residue levels. | in this paper we assessed the suitability of the charm hvs and a newly developed microbiological multiplate system as post-screening tests to confirm the presence of residues in raw milk at or near the maximum permissible residue level (mrl). the multiplate system is composed of bacillus stearothermophilus var. calidolactis plate at ph 8.0 for detection of beta-lactam antibiotics and tylosin, bacillus cereus plate at ph 6.0 for detection of tetracyclines, micrococcus luteus plate at ph 8.0 for d ... | 1999 | 9990703 |
| identification and semiquantitative estimation of antibiotics added to complete feeds, premixes, and concentrates. | classical microbiological methods for determining antimicrobial compounds in feeds are nonspecific. thus, there is a need to identify biological activity, and bioautography is used for this purpose. a routine method for detecting the following antimicrobial substances in feeds is described: avilamycin, avoparcin, zn-bacitracin, erythromycin, flavomycin, furazolidone, lasalocid, monensin, narasin, penicillin, salinomycin, spiramycin, tetracyclines, tylosin, and virginiamycin. carbadox can be dete ... | 1999 | 10028666 |
| isolation of endospore-forming bacilli toxic to culiseta longiareolata (diptera: culicidae) in jordan. | ten of 80 endospore-forming bacilli, isolated from various habitats of jordan, were found to be highly toxic to the 4th instar larvae of culiseta longiareolata (macquart). the bacilli were identified into the following species and strains: bacillus sphaericus (h6), b. sphaericus (h9a, 9b), b. cereus frankland and frankland, b. brevis migula and b. megaterium bary. bacillus cereus comprised 50% of the isolates. the toxic concentrations of these isolates against c. longiareolata ranged between 1.2 ... | 1999 | 10030033 |
| a randomly amplified polymorphic dna marker specific for the bacillus cereus group is diagnostic for bacillus anthracis. | aiming to develop a dna marker specific for bacillus anthracis and able to discriminate this species from bacillus cereus, bacillus thuringiensis, and bacillus mycoides, we applied the randomly amplified polymorphic dna (rapd) fingerprinting technique to a collection of 101 strains of the genus bacillus, including 61 strains of the b. cereus group. an 838-bp rapd marker (sg-850) specific for b. cereus, b. thuringiensis, b. anthracis, and b. mycoides was identified. this fragment included a putat ... | 1999 | 10049896 |
| pulsed-light inactivation of food-related microorganisms. | the effects of high-intensity pulsed-light emissions of high or low uv content on the survival of predetermined populations of listeria monocytogenes, escherichia coli, salmonella enteritidis, pseudomonas aeruginosa, bacillus cereus, and staphylococcus aureus were investigated. bacterial cultures were seeded separately on the surface of tryptone soya-yeast extract agar and were reduced by up to 2 or 6 log10 orders with 200 light pulses (pulse duration, approximately 100 ns) of low or high uv con ... | 1999 | 10049899 |
| separation and properties of two acetylacetoin reductases from bacillus cereus yuf-4. | the separation and purification of two kinds of acetylacetoin reductases (aacrs) from bacillus cereus yuf-4 were examined. nadph-linked aacr (aacr i) and nadh-linked aacr (aacr ii) were separated from each other by ammonium sulfate fractionation, deae-cellulose chromatography, and hydroxyapatite chromatography. the former was purified 3.4-fold with a yield of 10.0%, and the latter was purified 29-fold with a yield of 15.6%. the two enzymes differ from each other in some enzymic properties such a ... | 1999 | 10052142 |
| syntheses and biological activities of rebeccamycin analogues. introduction of a halogenoacetyl substituent. | in the course of structure-activity relationships on rebeccamycin analogues, a series of compounds bearing a halogenoacetyl substituent were synthesized with the expectation of increasing the interaction with dna, possibly via covalent reaction with the double helix. two rebeccamycin analogues bearing an acetyl instead of a bromoacetyl substituent were prepared to gain an insight into the role of the halogen atom. the new compounds show very little effect on protein kinase c and no covalent reac ... | 1999 | 10052965 |
| the certification of a reference material for the evaluation of methods for the enumeration of bacillus cereus. | a reference material containing bacillus cereus was certified by the community bureau of reference (bcr) for its number of colony-forming particles (cfp) in 0.1 ml reconstituted capsule solution. to this end, a batch of approximately 15,000 capsules was produced and tested for its homogeneity and stability. the variation in the number of cfp between capsules (homogeneity) was not found to be significantly different from a poisson distribution. stability was tested for extended periods at storage ... | 1999 | 10063626 |
| bacillus cereus spores in raw milk: factors affecting the contamination of milk during the grazing period. | psychrotrophic bacillus cereus is a limiting factor for the shelf-life of pasteurized milk, particularly during the grazing season. potential sources of contamination and factors that might affect the spore content of milk were studied in detail for a group of eight cows during three 2-wk study periods from june to september over 2 yr. the spore content of milk was strongly associated with the degree of contamination of the teats with soil. high water content of soil, low evaporation of water an ... | 1999 | 10068952 |
| behavior of pythium torulosum zoospores during their interaction with tobacco roots and bacillus cereus. | bacillus cereus uw85 suppresses seedling damping-off diseases caused by oomycetes and produces antibiotics that inhibit development of oomycetes in culture. the goal of this study was to determine how uw85 and its antibiotics affected the behavior of an oomycete, pythium torulosum, in its interaction with plant roots. we studied tobacco seedlings inoculated with zoospores of p. torulosum and uw85 culture, culture filtrate, washed cells, antibiotics (zwittermicin a or kanosamine), purified from c ... | 1999 | 10069854 |
| characterization of the mbl determinant and cloning of the spoiiid gene from bacillus cereus atcc 10987. | to examine the transcriptional start site of the putative bacillus cereus mbl gene, we have cloned and sequenced the upstream region of the previously reported b. cereus mbl determinant, which showed sequence similarity to the mreb morphogene from escherichia coli. primer extension analysis revealed the transcriptional start site of mbl to 259 bp upstream of the putative translational start site and showed that the mbl gene was expressed at 3, 6, and 10 h of growth after inoculation. antibodies ... | 1999 | 10069858 |
| evaluation of accuracy and repeatability of identification of food-borne pathogens by automated bacterial identification systems. | the performances of five automated microbial identification systems, relative to that of a reference identification system, for their ability to accurately and repeatedly identify six common food-borne pathogens were assessed. the systems assessed were the microlog system (biolog inc., hayward, calif.), the microbial identification system (mis; midi inc., newark, del.), the vitek system (biomérieux vitek, hazelwood, mo.), the microscan walkaway 40 system (dade-microscan international, west sacra ... | 1999 | 10074506 |
| lactoferrin inhibits bacillus cereus growth and heme analogs recover its growth. | the growth of bacillus cereus was markedly inhibited by the addition of lactoferrin and was recovered by the addition of fecl3. the growth inhibition was also reversed by the addition of erythrocytes and hemoglobin. b. cereus can use heme or heme-protein complex (hemoglobin-haptoglobin and hematin-albumin complexes) as iron sources in iron deficient conditions. therefore, b. cereus uses these heme or heme-protein complexes to prevent the growth inhibition of lactoferrin in vivo. | 1999 | 10077441 |
| detection of clostridium perfringens alpha toxin using a capture antibody elisa. | clostridium perfringens phospholipase c (plc), commonly known as alpha toxin, is the lethal, dermonecrotic toxin produced by all strains and is considered a major virulence factor in clostridial myonecrosis. we developed a capture antibody elisa that accurately and specifically quantitates alpha toxin produced by c. perfringens. another plc, derived from bacillus cereus, and culture filtrates from various bacterial species including clostridium bifermentans and clostridium novyi were not cross-r ... | 1999 | 10080352 |
| [bacillus cereus pneumonia]. | 1999 | 10088415 | |
| [bacillus cereus food poisoning]. | 1999 | 10088445 | |
| isolation and detection of listeria monocytogenes using fluorogenic and chromogenic substrates for phosphatidylinositol-specific phospholipase c. | the bcm listeria monocytogenes detection system (lmds) consists of a selective preenrichment broth (lmpeb), selective enrichment broth (lmseb), selective/differential plating medium (lmpm), and identification on a confirmatory plating medium (lmcm). the efficacy of the bcm lmds was determined using pure cultures and naturally and artificially contaminated environmental sponges. the bcm lmpeb allowed the growth of listeria and resuscitation of heat-injured l. monocytogenes. the bcm lmseb, which c ... | 1999 | 10090243 |
| efficacy of oxonia active against selected spore formers. | alternatives to hydrogen peroxide are being sought for use in aseptic packaging systems because this sterilant is efficacious at temperatures higher than some of the newer packaging materials can tolerate. earlier in this century, peracetic acid was known to be bactericidal, sporicidal, and virucidal but was not widely used because of handling, toxicity, and stability problems. sanitizer suppliers have capitalized on the efficacy of hydrogen peroxide, acetic acid, and peracetic acid stabilized w ... | 1999 | 10090246 |
| a novel extracellular diterpenoid with antibacterial activity from the cyanobacterium nostoc commune. | a novel extracellular metabolite with an unprecedented diterpenoid skeleton, 8-[(5-carboxy-2-hydroxy)benzyl]-2-hydroxy-1,1,4a,7, 8-pentamethyl-1,2,3,4,4a,6,7,8,8a,9,10,10a-dodecahydrophenanthrene , has been isolated from the culture medium of the terrestrial cyanobacterium nostoc commune vaucher (eawag 122b) by means of bioguided isolation. the molecule was designated as noscomin. the structure was determined by spectroscopic methods, mainly nmr and mass spectrometry. noscomin exhibited antibact ... | 1999 | 10096870 |
| cytologic findings in vitreous fluids. analysis of 74 specimens. | to review the cytologic findings of vitreous specimens and propose a simplified approach to them. | 1999 | 10097710 |
| macromolecular antimicrobial glycoprotein, achacin, expressed in a methylotrophic yeast pichia pastoris. | a cdna encoding achacin, an antimicrobial glycoprotein from the body surface mucus of giant african snail achacina fulica férussac, was expressed in a methylotrophic yeast, pichia pastoris, and recombinant achacin (rach) was secreted in yeast minimal medium in a polyglycosylated form with 80 kda. carbohydrate analysis revealed that the glycosylated moiety of rach was composed of 50 mol mannose and 2 mol n-acetylglucosamine residues. antimicrobial activity using escherichia coli and staphylococcu ... | 1999 | 10217406 |
| genome organization is not conserved between bacillus cereus and bacillus subtilis. | the opportunistic pathogen bacillus cereus is the genetically stable member of a group of closely related bacteria including the insect pathogen bacillus thuringiensis and the mammalian pathogen bacillus anthracis. physical maps of b. cereus and b. thuringiensis strains show considerable variations in discrete parts of the chromosome, suggesting that certain genome regions are more prone to rearrangements. b. cereus belongs to the same subgroup of bacillus species as bacillus subtilis, by both p ... | 1999 | 10217496 |
| genetic basis in plants for interactions with disease-suppressive bacteria. | plant health depends, in part, on associations with disease-suppressive microflora, but little is known about the role of plant genes in establishing such associations. identifying such genes will contribute to understanding the basis for plant health in natural communities and to new strategies to reduce dependence on pesticides in agriculture. to assess the role of the plant host in disease suppression, we used a genetic mapping population of tomato to evaluate the efficacy of the biocontrol a ... | 1999 | 10220371 |
| mono- and binuclear zn2+-beta-lactamase. role of the conserved cysteine in the catalytic mechanism. | when expressed by pathogenic bacteria, zn2+-beta-lactamases induce resistance to most beta-lactam antibiotics. a possible strategy to fight these bacteria would be a combined therapy with non-toxic inhibitors of zn2+-beta-lactamases together with standard antibiotics. for this purpose, it is important to verify that the inhibitor is effective under all clinical conditions. we have investigated the correlation between the number of zinc ions bound to the zn2+-beta-lactamase from bacillus cereus a ... | 1999 | 10224083 |
| an organosulfur compound isolated from oil-macerated garlic extract, and its antimicrobial effect. | an organosulfur compound was isolated from oil-macerated garlic extract by silica gel column chromatography and preparative tlc. from the results of nmr, ir, and ms analyses, its structure was determined as e-4,5,9-trithiadeca-1,7-diene-9-oxide (iso-e-10-devinylajoene, iso-e-10-da). this compound was different from e-4,5,9-trithiadeca-1,6-diene-9-oxide (e-10-devinylajoene, e-10-da) only in the position of a double bond. iso-e-10-da had antimicrobial activity against gram-positive bacteria, such ... | 1999 | 10227149 |
| corona plasma discharge for rapid analysis of microorganisms by mass spectrometry. | corona plasma discharge provides a rapid and reliable tool for release of biomarkers from gram negative and positive bacteria, spores and viruses for characterization by mass spectrometry. | 1999 | 10230068 |
| mic231, a naturally occurring mobile insertion cassette from bacillus cereus. | recent dissection of numerous plasmids and transposable elements has given more credence to the modular organization of these genetic and genomic entities. although many variations on each theme exist, the number of basic functional cassettes is thought to be relatively limited. in this paper, a novel type of mobile cassette is described. a naturally occurring assemblage consisting of two left is231 ends flanking a d-stereospecific endopeptidase (adp) gene was found in several natural isolates o ... | 1999 | 10320586 |
| synthesis of some new biologically-active coumarin derivatives. | coumarin and 6-nitrocoumarin hydrazones (2a,b), respectively, were prepared via the reaction of 2-thiocoumarin (1a,b) derivatives with hydrazine hydrate. the hydrazones were used as key intermediates for the preparation of some benzopyrano[2,3-c]pyrazoles (21-24) through the reaction of different acyl halides and subsequent cyclization in n,n-dimethylaniline. benzopyrano[2,3-c]pyrazole-3-thione (25a,b) was prepared by the reaction of 1a with cs2 on which some alkylation, acylation and mannich re ... | 1999 | 10321030 |
| [bacillary endophthalmitis. four case reports]. | bacillary endophthalmitis occurring after penetrating ocular trauma with an intraocular foreign body is always associated with poor visual outcome. bacilli cause fulminant infection associated with tissue damage in the intraocular structures. | 1999 | 10337596 |
| toward functional genomics in bacteria: analysis of gene expression in escherichia coli from a bacterial artificial chromosome library of bacillus cereus. | as the study of microbes moves into the era of functional genomics, there is an increasing need for molecular tools for analysis of a wide diversity of microorganisms. currently, biological study of many prokaryotes of agricultural, medical, and fundamental scientific interest is limited by the lack of adequate genetic tools. we report the application of the bacterial artificial chromosome (bac) vector to prokaryotic biology as a powerful approach to address this need. we constructed a bac libra ... | 1999 | 10339608 |
| nisin in milk sensitizes bacillus spores to heat and prevents recovery of survivors. | decimal reduction times (d values) were determined for bacillus cereus t spores and b. stearothermophilus atcc 12980 spores in skim milk supplemented with various concentrations (0, 2,000, and 4,000 iu/ml) of the bacteriocin nisin by using an immersed, sealed capillary tube procedure. for both organisms, the addition of nisin lowered the apparent d values. for b. cereus, the addition of 2,000 iu of nisin per ml to skim milk before heating significantly (p< or =0.05) lowered the apparent d value ... | 1999 | 10340670 |
| pathological effect of synthetic cereulide, an emetic toxin of bacillus cereus, is reversible in mice. | cereulide is the causative toxin of the emetic type of food-borne illness caused by bacillus cereus. this toxin was previously shown to be associated with fulminant liver failure in a human case. mice were injected i.p. with synthetic cereulide and the development of histopathological changes was examined. hepatocytes showed mitochondrial swelling with loss of cristae, and dose-dependent increase of small fatty droplets. these microsteatotic hepatocytes were distributed mainly in the pericentral ... | 1999 | 10340720 |
| synthesis, mode of action, and biological activities of rebeccamycin bromo derivatives. | bromo analogues of the natural metabolite rebeccamycin with and without a methyl substituent on the imide nitrogen were synthesized. the effects of the drugs on protein kinase c, the binding to dna, and the effect on topoisomerase i were determined. the drugs' uptake and their antiproliferative activities against p388 leukemia cells sensitive and resistant to camptothecin, their antimicrobial activity against a gram-positive bacterium (b. cereus), and their anti-hiv-1 activity were measured and ... | 1999 | 10346933 |
| isolation, partial purification and characterization of a bacteriocin produced by a newly isolated bacillus subtilis strain. | a wild type micro-organism producing antibacterial substances has been isolated from a chinese fermented soybean seasoning and identified as bacillus subtilis. a crude antibacterial preparation (cabp) was obtained by ammonium sulphate precipitation. isoelectric focusing assay revealed at least four antimicrobial components in the cabp. however, in sds-page analysis, only one peptide band displayed antimicrobial activity against pathogenic bacillus cereus and listeria monocytogenes. this inhibito ... | 1999 | 10347890 |
| improved cytotoxicity assay for bacillus cereus diarrhoeal enterotoxin. | an improved mccoy cell cytotoxicity assay for bacillus cereus diarrhoeal toxin, which includes a staining procedure in addition to visual examination, was developed and the method was compared with two commercially available kits (oxoid bcet-rpla and tecra bde-via). a total of 71 strains of 15 different bacillus, brevibacillus and paenibacillus species, including 16 strains of b. cereus from outbreaks of food-borne illness, were evaluated for toxin production. eleven of the outbreak strains exhi ... | 1999 | 10347895 |
| crystal structure of beta-amylase from bacillus cereus var. mycoides at 2.2 a resolution. | the crystal structure of beta-amylase from bacillus cereus var. mycoides was determined by the multiple isomorphous replacement method. the structure was refined to a final r-factor of 0.186 for 102,807 independent reflections with f/sigma(f) > or = 2.0 at 2.2 a resolution with root-mean-square deviations from ideality in bond lengths, and bond angles of 0.014 a and 3.00 degrees, respectively. the asymmetric unit comprises four molecules exhibiting a dimer-of-dimers structure. the enzyme, howeve ... | 1999 | 10348915 |
| structure of raw starch-digesting bacillus cereus beta-amylase complexed with maltose. | the crystals of beta-amylase from bacillus cereus belong to space group p21 with the following cell dimensions: a = 57.70 a, b = 92.87 a, c = 65.93 a, and beta =101.95 degrees. the structures of free and maltose-bound beta-amylases were determined by x-ray crystallography at 2.1 and 2.5 a with r-factors of 0.170 and 0.164, respectively. the final model of the maltose-bound form comprises 516 amino acid residues, four maltose molecules, 275 water molecules, one ca2+, one acetate, and one sulfate ... | 1999 | 10353816 |
| comparison between automatic ribotyping and random amplified polymorphic dna analysis of bacillus cereus isolates from the dairy industry. | discrimination by automatic ribotyping and random amplified polymorphic dna pcr, rapd, was compared for 40 different b. cereus dairy isolates, 4 different b. mycoides isolates and 6 culture collection strains. rapd-pcr has previously shown to be useful for tracing contamination routes of b. cereus to milk. automatic ribotyping using ecori and pvuii separated the b. cereus and b. mycoides isolates/strains into 36 different ribotypes. rapd-typing with primers generated 40 different rapd-profiles. ... | 1999 | 10357283 |
| influence of temperature shifts on survival, growth, and toxin production by psychrotrophic and mesophilic strains of bacillus cereus in potatoes and chicken gravy. | a study was done to determine the influence of temperature on growth and toxin production characteristics of psychrotrophic and mesophilic strains of bacillus cereus when inoculated into mashed potatoes and chicken gravy containing various concentrations of sodium chloride and held at temperatures different from those at which cells had been cultured. logarithmic growth phase cells (10 h, 30 degrees c) of psychrotrophic (f3802a/84) and mesophilic (b4ac-1) strains of bacillus cereus were inoculat ... | 1999 | 10359488 |
| modeling the inhibitory effects of organic acids on bacteria. | the inhibitory effect of acids on microbial growth has long been used to preserve foods from spoilage. while much of the effect can be accounted for by ph, it is well known that different organic acids vary considerably in their inhibitory effects. because organic acids are not members of a homologous series, but vary in the numbers of carboxy groups, hydroxy groups and carbon-carbon double bonds in the molecule, it has typically not been possible to predict the magnitude, or in some cases even ... | 1999 | 10359489 |
| plcr is a pleiotropic regulator of extracellular virulence factor gene expression in bacillus thuringiensis. | members of the bacillus cereus group (b. anthracis, b. cereus, b. mycoides and b. thuringiensis) are well-known pathogens of mammals (b. anthracis and b. cereus) and insects (b. thuringiensis). the specific diseases they cause depend on their capacity to produce specific virulence factors, such as the lethal toxin of b. anthracis and the cry toxins of b. thuringiensis. however, these bacillus spp. also produce a variety of proteins, such as phospholipases c, which are known to act as virulence f ... | 1999 | 10361306 |
| nosocomial pseudoepidemic caused by bacillus cereus traced to contaminated ethyl alcohol from a liquor factory. | from september 1990 to october 1990, 15 patients who were admitted to four different departments of the national taiwan university hospital, including nine patients in the emergency department, three in the hematology/oncology ward, two in the surgical intensive care unit, and one in a pediatric ward, were found to have positive blood (14 patients) or pleural effusion (1 patient) cultures for bacillus cereus. after extensive surveillance cultures, 19 additional isolates of b. cereus were recover ... | 1999 | 10364598 |
| time for a fresh look at the bacterial chromosome. | 1999 | 10366858 | |
| pathogenesis of gram-positive bacterial endophthalmitis. | the severity of endophthalmitis has been associated generally with the virulence of the offending pathogen. however, precisely what constitutes the virulence in intraocular infections remains ill defined. we therefore sought to identify the basis for virulence for three common ocular pathogens (bacillus cereus, enterococcus faecalis, and staphylococcus aureus) in terms of intraocular growth rates, bacterial localization patterns, and the contribution of cell walls and secreted products to the pa ... | 1999 | 10377112 |
| role of hemolysin bl in the pathogenesis of extraintestinal bacillus cereus infection assessed in an endophthalmitis model. | bacillus cereus is a rare cause of serious human infection but, paradoxically, causes one of the most severe posttraumatic or endogenous infections of the eye, endophthalmitis, which frequently results in blindness. the virulence of b. cereus endophthalmitis historically has been attributed to toxin production. we therefore sought to examine the contribution of the dermonecrotic toxin, hemolysin bl, to the pathogenesis of b. cereus infection in an endophthalmitis system that is highly amenable t ... | 1999 | 10377113 |
| early versus late removal of retained intraocular foreign bodies. | to compare early versus late removal of retained intraocular foreign bodies (iofbs). | 1999 | 10380023 |
| evaluation of a modified enzymatic test for the detection of tetracyclines in milk. | the tetracycline galactosidase (tg) test, a new method for the detection of tetracycline residues in raw milk based on the inhibition of beta-galactosidase biosynthesis in escherichia coli, was previously validated with spiked milk samples. it has now been applied to milk from cows treated with oxytetracycline. in view of the occurrence of false positives, related to highly elevated somatic cell counts (>10(6)/ml), the improved tg test was developed, in which a heating step (80 degrees c, 15 min ... | 1999 | 10382652 |
| bacterial contamination of ready-to-eat foods and fresh products in retail shops and food factories. | raw vegetables cut for salad, cooked salad, cooked rice, boiled noodles, bean curd, and cooked japanese foods were purchased in 27 retail shops in tokyo. intact vegetables before being processed and ready-to-eat fresh salad products were obtained from two food factories located in the suburbs of tokyo. two hundred thirty-eight retail samples, 137 samples of intact vegetables, and 159 samples of fresh products were examined for aerobic plate count (apc), coliforms, escherichia coli, listeria spp. ... | 1999 | 10382654 |
| characterization, production, and purification of leucocin h, a two-peptide bacteriocin from leuconostoc mf215b. | leuconostoc mf215b was found to produce a two-peptide bacteriocin referred to as leucocin h. the two peptides were termed leucocin halpha and leucocin hbeta. when acting together, they inhibit, among others, listeria monocytogenes, bacillus cereus, and clostridium perfringens. production of leucocin h in growth medium takes place at temperatures down to 6 degrees c and at ph below 7. the highest activity of leucocin h in growth medium was demonstrated in the late exponential growth phase. the ba ... | 1999 | 10387116 |
| specific detection of the gene for the extracellular neutral protease of bacillus cereus by pcr and blot hybridization. | a pair of primers and a gene probe for the amplification and detection of the bacillus cereus neutral protease gene (nprc) were developed. specificity for the npr genes of the b. cereus group members b. cereus, b. mycoides, and b. thuringiensis was shown. restriction polymorphism patterns of the pcr products confirmed the presence of the nprc gene in all three species. | 1999 | 10388725 |