Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| cultivation of mycobacterium x from mycobacterium leprae-infected tissues in propane-tetradecane-humic acid medium. | 1984 | 6231260 | |
| hla-d identity in a family with multiple cases of multibacillary leprosy. | 1984 | 6231441 | |
| [control of leprosy]. | 1984 | 6238608 | |
| [symposium on mechanisms of host defense from fundamental and clinical medicine. 5. genetic control of immune response in humans and its clinical significance]. | 1984 | 6241938 | |
| leprosy vaccine. | 1984 | 6242878 | |
| generation and characterization of monoclonal antibodies to the phenolic glycolipid of mycobacterium leprae. | nine cloned cell lines producing antibodies to the unique phenolic glycolipid of mycobacterium leprae have been established as a result of fusions with spleens from mice immunized with the glycolipid complexed with methylated bovine serum albumin. one of the antibodies was relatively nonspecific, binding to a related glycolipid from mycobacterium kansasii, but the remaining antibodies were specific for the m. leprae lipid. some of the antibodies required the intact (trisaccharide) carbohydrate p ... | 1984 | 6360894 |
| chemical synthesis and serology of disaccharides and trisaccharides of phenolic glycolipid antigens from the leprosy bacillus and preparation of a disaccharide protein conjugate for serodiagnosis of leprosy. | we examined the structural requirements within the species-specific 3,6-di-o-methyl-beta-d-glucopyranosyl-(1 leads to 4)-2,3-di-o-methyl- alpha-l-rhamnopyranosyl-(1 leads to 2)-3-o-methyl-alpha-l-rhamnopyranose unit of the phenolic glycolipid i antigen of mycobacterium leprae for binding to anti-glycolipid immunoglobulin m from human leprosy sera. we used chemically defined, partially deglycosylated fragments of phenolic glycolipid i, two other minor m. leprae-specific phenolic glycolipids (thos ... | 1984 | 6360898 |
| evaluation of mycobacterium leprae immunogenicity via adoptive transfer studies. | the immune response of mice to live, heat-killed, or autoclaved mycobacterium leprae was investigated. after sensitization with 10(7) organisms in each group, recipient mice were transfused with the sensitized splenocytes 28 days later. a selected number of these mice were infected with 5 x 10(3) m. leprae, and the remaining animals were sacrificed at scheduled intervals for evidence of cell-mediated immunity to the m. leprae cell extract. data from these and the bacteriological assays showed th ... | 1984 | 6360910 |
| biological activities of a murine t-cell clone with reactivity to mycobacterium leprae. | mice were immunized subcutaneously with killed mycobacterium leprae in incomplete freund's adjuvant and draining lymph nodes removed. lymph node cells were propagated in vitro and cloned at limiting dilution in the presence of syngeneic accessory cells, antigen, and t-cell growth factor. cloned t cells were restricted by the h-2i-a sublocus. in vitro interaction(s) of cloned t cells with accessory cells presenting m. leprae-derived determinants resulted in t-cell proliferation, interleukin secre ... | 1984 | 6362895 |
| low t lymphocyte responsiveness to mycobacterium leprae antigens in association with hla-dr3. | the type of leprosy which develops after infection with mycobacterium leprae is influenced by the presence or absence of hla-dr3, as has been demonstrated in an ethnic group originating from surinam. in the present study we investigated in this same ethnic group the role of hla-dr, and of hla-dr3 in particular, in monocyte-t cell interactions during leprosy specific proliferative responses in vitro. hla-dr3 heterozygous t cells from tuberculoid leprosy patients were cultured with antigen and eit ... | 1984 | 6362932 |
| an attempt to inoculate mycobacterium leprae into 'rhino' mice. | 1984 | 6365647 | |
| defective production of monocyte-activating cytokines in lepromatous leprosy. | we have examined the capacity of monocytes from patients with leprosy to undergo activation and the capacity of mononuclear cells from these patients to incorporate [3h]thymidine and produce monocyte-activating cytokines. monocytes from patients with either lepromatous or tuberculoid leprosy were activated by concanavalin a (con a)-induced mononuclear cell supernatants generated from the leukocytes of a normal person. monocytes activated by these supernatants strongly inhibited l. pneumophila mu ... | 1984 | 6366107 |
| lymphocyte suppression in leprosy induced by unique m. leprae glycolipid. | leprosy remains a significant medical and social problem in many developing countries. the varied forms of the disease form a spectrum. at one pole, tuberculoid leprosy, patients develop high levels of cell-mediated immunity which results in the killing and clearing of bacilli in the tissues. at the lepromatous pole, patients exhibit a selective immunological unresponsiveness to antigens of mycobacterium leprae so that the organisms inexorably multiply in the skin. we have suggested that in lepr ... | 1984 | 6366573 |
| parasitism of antigen presenting cells in hyperbacillary leprosy. | full thickness skin biopsies from four patients with borderline lepromatous leprosy (bl leprosy) have been examined. immunohistological techniques have been employed to analyse the non-lymphoid mononuclear cells present in the dermal infiltrates associated with the bl lesions. this analysis was performed using three monoclonal antibodies, rfd2 (recognizing macrophages), rfd1 (recognizing interdigitating cells) and na1/34 (recognizing langerhans cells). it was found that the vast majority of non- ... | 1984 | 6368061 |
| acid-fast bacilli on buffy coat smears in the acquired immunodeficiency syndrome: a lesson from hansen's bacillus. | a patient with the acquired immunodeficiency syndrome was found to have a continuous bacillemia of mycobacterium avium-intracellulare by examination of kinyoun-stained buffy coat smears. there were 29 cells/cu mm that contained acid-fast bacilli (afb) and 1.5 x 10(5) afb/ml of whole blood. the cells of the reticuloendothelial system were engorged with afb, suggesting reticuloendothelial saturation. the peripheral blood involvement and magnitude of the mycobacterial burden are analogous to lepros ... | 1984 | 6199851 |
| collection method for mycobacterium leprae from infected armadillo liver. | leprosy bacilli were separated from infected armadillo liver almost free from tissue contaminants by a percoll gradient centrifugation. the yield of bacilli was 46.7%. this is a very simple and effective method without enzyme treatment. | 1984 | 6368427 |
| variation of superoxide dismutase levels in extracts of mycobacterium leprae from armadillo liver. | recent improvements in the sensitivity of assay methods for superoxide dismutase (sod) have enabled the detection of this enzyme in 18 cell-free extracts of purified mycobacterium leprae. by converting back to units of sod obtained in the cytochrome c-based method previously used in work on this enzyme in mycobacteria, it was shown that extracts of m. leprae had 0.15-3.84 u sod/mg protein (this study). a mean value of 1.31 u/mg protein was calculated. it was not possible to find any factors whic ... | 1984 | 6368428 |
| 2-acetylpyridine thiosemicarbazones and mycobacterium leprae. | four 2-acetylpyridine thiosemicarbazones were tested in mice against mycobacterium leprae by the kinetic method and found to be nearly inactive in a dosage of 0.05% in the diet. at the same dosage, thiacetazone, as a positive control, exhibited its expected activity. | 1984 | 6368429 |
| immunity to leprosy and the mitsuda reaction. | 1984 | 6368430 | |
| application of monoclonal antibodies towards immunological studies in leprosy. | 1984 | 6369053 | |
| effects of mycobacterium leprae antigens on the in vitro responsiveness of mononuclear cells from armadillos to concanavalin-a. | 1984 | 6369054 | |
| skin test activity of an antigen fraction prepared from mycobacterium leprae compared with standard lepromin and tuberculin ppd in leprosy patients. | 1984 | 6369055 | |
| an alternative route for infecting armadillos with mycobacterium leprae. | a nine-banded armadillo was inoculated with mycobacterium leprae in both hind footpads. the animals were usually inoculated intravenously, or intradermally in the abdominal skin. profuse multiplication of the bacilli occurred at the injection sites after more than two years. eventually bacteraemia developed, and large numbers of the organisms were found in skin biopsies and in lymph nodes. there was limited dissemination of the bacteria into the spleen and the liver, and peripheral nerve invasio ... | 1984 | 6369076 |
| immunization against leprosy: progress and prospects. | the limitations of the current approach to leprosy control through mass treatment of patients are well recognized. the long incubation period of the disease, the insidious onset, the chronic course, and the need for prolonged treatment have made control a formidable task. the recent years have seen tremendous progress in the field of immunology of leprosy, and the availability of large quantities of mycobacterium leprae, grown in the nine-banded armadillo, has given impetus to the search for a v ... | 1984 | 6370487 |
| antibodies to mycobacterial arabinomannan in leprosy: correlation with reactional states and variation during treatment. | an enzyme-linked immunosorbent assay was used to measure antibody to mycobacterial arabinomannan in serial serum specimens obtained over the initial 12-31 months of therapy from nine patients with leprosy. the antibody level in pretreatment sera was directly proportional to the quantity of mycobacterium leprae present in each patient as assessed by six-site scrapings (r = 0.75). the three patients with the lowest antibody levels (od 0.1-0.3) had uncomplicated courses and their levels declined sl ... | 1984 | 6373624 |
| adenosine triphosphate content of mycobacterium leprae from leprosy patients. | mycobacterium leprae obtained from randomly selected lepromatous leprosy patients were used to evaluate the atp assay technique for detecting viability of these cells. the findings were further confirmed by the standard mouse foot pad technique. while the latter takes about 8-12 months to obtain any valid information on the status of m. leprae, the atp data can be generated within hours and at much lower cost. it is hoped that the atp data could also instantaneously identify viable bacilli from ... | 1984 | 6373626 |
| attempts at cultivation of mycobacterium leprae in macrophages from susceptible animal hosts. | macrophages from nude mice, nude rats, and armadillos were cultured in vitro and examined for their ability to support mycobacterium leprae. no significant growth of this organism were observed after over 200 days of culture. no significant benefit was derived from modifying culture conditions or from variations in the source of macrophages or the source of m. leprae. | 1984 | 6373627 |
| ultrastructural features of macrophages of armadillos infected with actively multiplying mycobacterium leprae. | experimental leprosy lesions in the armadillo (dasypus novemcinctus) were studied by freeze etching and ultrathin sectioning. infected macrophages have distinct intracytoplasmic foamy structures in the form of spherical droplets accumulated around multiplying bacilli. this finding is the same as those observed in human lepra cells and nude mice macrophages infected with m. leprae. | 1984 | 6373628 |
| electron microscopic study of leprosy in a mangabey monkey (natural infection). | ultrastructural features of the leproma of a) a naturally infected mangabey monkey, and lepromas and liver of b) a passage mangabey monkey, c) a rhesus monkey, d) an african green monkey, and e) a nine-banded armadillo inoculated with leprosy bacilli isolated from the leproma of a naturally infected mangabey monkey were studied by the freeze-etching technique. the size, shape, and ultrastructural features of leprosy bacilli in the phagolysosomes of macrophages in all of these samples were essent ... | 1984 | 6373629 |
| multiple axonal myelination in the experimental mouse leprosy model. | 1984 | 6373630 | |
| oxidation of carbon sources through the tricarboxylic acid cycle in mycobacterium leprae grown in armadillo liver. | all the enzymes of the tricarboxylic acid cycle have now been demonstrated in extracts of mycobacterium leprae grown in armadillo liver. many were also present in homogenates of host-tissue, but biochemical evidence is presented which indicates that all enzymes detected in extracts from m. leprae were authentic bacterial enzymes. further evidence for a complete tricarboxylic acid cycle in m. leprae was obtained by first establishing that citrate could be taken up and catabolized by whole m. lepr ... | 1984 | 6374022 |
| in vitro studies on the effect of levamisole in lepromatous leprosy. | our previous studies have demonstrated a defective macrophage response to m. leprae in lepromatous leprosy patients. in the present study we report the restoration of fc receptor and hla-dr antigen expression as well as antigen specific macrophage-lymphocyte interaction on treatment with levamisole in vitro. these results indicate that levamisole activates the macrophages which in turn results in an improved cell mediated immune response in lepromatous leprosy. this may also be applicable in oth ... | 1984 | 6374154 |
| [effect of ultrasonic waves on the growth intensity of mycobacterium leprae in liquid nutrient media]. | ultrasound-treated m. leprae grow in liquid culture media at a greater rate than untreated ones. ultrasound-treated m. leprae retain their capacity for intensive growth after their passage in mice. | 1984 | 6375218 |
| humans respond predominantly with igm immunoglobulin to the species-specific glycolipid of mycobacterium leprae. | the immunoglobulin classes of the antibody response to the species-specific phenolic glycolipid antigen of mycobacterium leprae have been characterized for serum specimens from 78 patients with leprosy. these patients included the entire clinical spectrum from paucibacillary to multibacillary disease, including polar tuberculoid (tt; 11 patients), borderline tuberculoid (bt; 15), borderline (bb; 17), borderline lepromatous (bl; 13), and lepromatous (ll; 22)--clinical classifications according to ... | 1984 | 6376654 |
| antigen specific macrophage-lymphocyte interaction in lepromatous leprosy. | peripheral blood derived macrophages from lepromatous leprosy patients were unable to interact with lymphocytes in the presence of m. leprae. this lack of interaction is probably not associated with membrane hla-dr antigens since trypsin and colchicine restored m. leprae induced depression in the latter but were unable to bring about a positive interaction. two possible defects exist therefore in the lepromatous macrophage. these are an innate inability to process and present m. leprae antigens ... | 1984 | 6376810 |
| [advances in the chemotherapy of leprosy]. | the chemotherapy of multibacillary leprosy with dapsone alone has resulted after 15-20 years in the selection and the diffusion of dapsone resistant mycobacterium leprae. to overcome dapsone resistance, to prevent the selection of organisms resistant to other drugs and to kill the largest proportion of sensitive m. leprae, the chemotherapy of leprosy must rely, as chemotherapy of tuberculosis, upon the combination of several drugs. rifampicin is included in all drug combinations recommended by w ... | 1984 | 6377002 |
| anti-mycobacterium leprae antibodies in urine from lepromatous patients examined by crossed immunoelectrophoresis and radioimmunoassay. | precipitating anti-mycobacterium leprae antibodies were found in concentrated urine samples from 21 out of 42 lepromatous patients. these antibodies were directed against m. leprae antigens 5, 6, and 7. in a radioimmunoassay for anti-m. leprae antibodies, 90% of these patients had higher antibody levels in their urine than control persons. there was a positive correlation between anti-m. leprae antibody levels in serum and urine. the advantages of using atraumatically collected samples like urin ... | 1984 | 6377478 |
| immunochemical characterization of mycobacterium leprae antigens by the sds-polyacrylamide gel electrophoresis immunoperoxidase technique (sgip) using patients' sera. | in this study the sds-polyacrylamide gel electrophoresis immunoperoxidase (sgip) assay was used for characterizing the antigenic components of mycobacterium leprae using patients' sera. this technique involved the separation of mycobacterial sonicates on sds-polyacrylamide gels, longitudinal sectioning of the gels, incubation with patients' sera and visualization of the antigen-antibody complexes by the indirect immunoperoxidase technique. a number of antigens present in m. leprae sonicates were ... | 1984 | 6378452 |
| c-reactive protein and apob containing lipoproteins are associated with mycobacterium leprae in lesions of human leprosy. | skin biopsies from patients with leprosy across the spectrum from tuberculoid (tt) to lepromatous (ll), including histoid lepromas and erythema nodosum leprosum (enl) reactions, were stained immunohistochemically for the presence of c-reactive protein (crp) and the apolipoprotein, apob. mycobacterium leprae bacillary material comprising cell walls, cytoplasmic and soluble components was present with increasing abundance towards the lepromatous end of the spectrum and always stained positively wi ... | 1984 | 6378453 |
| the effect of cortisone on mycobacterium leprae-infected mice. | 1984 | 6379352 | |
| dapsone-resistant leprosy. | 1984 | 6379353 | |
| electrophysiological studies of the sciatic nerves in mycobacterium leprae foot pad-injected rats. | this study tested the possibility of developing an experimental model of neuropathy in female wistar rats inoculated with mycobacterium leprae in the foot pad and assessed by repeated electrophysiological methods. m. leprae multiplied in the rats but considerably less than in simultaneously inoculated mice. no acid-fast bacilli were found in nerves. motor and sensory conduction velocities remained normal at the thigh level of the sciatic nerve. at the leg, they decreased significantly bilaterall ... | 1984 | 6384077 |
| lactate dehydrogenase in mycobacterium leprae grown in armadillo liver. | lactate dehydrogenase (ldh) was detected in extracts of untreated and naoh-treated mycobacterium leprae. since armadillo liver ldh isoenzymes with a similar electrophoretic mobility were shown to be considerably more sensitive to inhibition by oxamate than ldh in m. leprae extracts, it was confirmed that m. leprae grown in armadillo liver has its own ldh. neither the activity of ldh in m. leprae nor its electrophoretic mobility supported the tentative suggestion that an "anomolous" ldh isoenzyme ... | 1984 | 6384078 |
| unresponsiveness to mycobacterium leprae in lepromatous leprosy in vitro: reversible or not? | 1984 | 6384079 | |
| modification of the fluorescent staining method for mycobacterial cells. | 1984 | 6384149 | |
| the mouse footpad test--sensitive to small proportions of drug-resistant bacilli in a sample of m. leprae. | in experiments at the radda barnen research laboratories of the slr & tc karigiri, the mouse footpad test was demonstrated to detect dds-resistant m.leprae even if as few as 0.1% (1 in 1000) of the m. leprae tested were dds-resistant. the mouse footpad test appears to be sensitive to minute proportions of drug-resistant bacilli in samples of m. leprae tested. | 1984 | 6384379 |
| human vaccination studies in normal and contacts of leprosy patients. | 1984 | 6384380 | |
| mechanism of immunosuppression in leprosy--macrophage membrane alterations. | l-lysate induced macrophage membrane alteration was studied using 3 membrane markers: (i) fc receptor, (ii) concanavalin a (con a) receptor, and (iii) m. leprae adherence to macrophage membrane. the data indicate that l-lysate induces membrane perturbation of normal macrophages. the alteration can be reversed with trypsin and colchicine. membrane alteration observed may lead to defective macrophage participation in a cell-mediated immune reaction. | 1984 | 6384522 |
| cell-mediated immunity in mice treated with mycobacterium leprae or with macrophages harbouring m. leprae. | following treatment of balb/c or c3h/hen mice in the hind footpads with irradiated mycobacterium leprae, a marked enhancement of natural killer (nk) activity was observed in cells from the draining popliteal lymph node or from the spleen. nk activity was further enhanced when the treatment consisted of killed m. leprae which had been incorporated into mouse peritoneal macrophages. this effect was noted as early as 2 weeks after treatment and persisted for at least 9 weeks. lymphoblastic transfor ... | 1984 | 6385816 |
| reversal by interleukin-2 of the t cell unresponsiveness of lepromatous leprosy to mycobacterium leprae. | in some subjects mycobacterium leprae causes disseminated (lepromatous) disease. such subjects show both in vivo and in vitro deficient t cell responses to m. leprae, but not to other antigens. we have recently shown that lepromatous peripheral blood mononuclear cells (pbmc) failed to produce interleukin 2 (il-2) in response to m. leprae and that t cell-conditioned media (tcm) can reverse the t cell unresponsiveness in a majority of lepromatous leprosy patients (haregewoin et al. 1983). here we ... | 1984 | 6386665 |
| comparison of biochemical characterisation of icrc bacilli with m. leprae: effect of substrate alteration in the medium. | icrc-bacilli strain c-44 when grown in dubos medium of its equivalent, express m. avium taxonomic biochemical characters. assuming that difference in characters of m. leprae and icrc bacilli, could be due to 'in vivo' and 'in vitro' milieu, we altered the substrates in the medium. the bacilli grow well in the new medium containing selenium, ferric nitrate, magnesium chloride and deleting tween 80. the icrc strain c-44 grown in new medium expressed characters: 9/10 similarity with m. leprae. the ... | 1984 | 6387003 |
| antigeneic cross-reactivity between icrc-bacilli and m. leprae--"in vitro" evaluation. | leucocyte migration inhibition, in presence of specific particulate antigen, is a good correlate of cmi. it can detect small differences in related antigens. in the present study, lmi was used to study the cross-reactivity between icrc bacilli and m. leprae and also to examine the antigenic relationship amongst different icrc isolates. the study showed a close antigenic cross-reactivity in lepromin and icrc-in. lmi has brought out strain differences in two icrc culture isolates, c-44 and c-75. t ... | 1984 | 6387004 |
| hypothesis: possible idiotypic suppression of cell-mediated immunity in lepromatous leprosy. | 1984 | 6387354 | |
| the discovery of mycobacterium leprae. a medical achievement in the light of evolving scientific methods. | the discovery of m. leprae by g. h. armauer hansen (1841-1912) in 1873 represents a link in a chain of development in international medicine that was influenced by two main concepts, namely, that germs may be causes of disease and that social conditions may be related to disease, either as causes or consequences or both. hansen's work is also a link in a chain of research on leprosy in norway. hansen met with serious challenges in addition to those that were purely scientific. to prove the causa ... | 1984 | 6388392 |
| three histologic fixatives for the demonstration of mycobacterium leprae. | 1984 | 6388395 | |
| mycobacterium leprae within a squamous cell carcinoma. | 1984 | 6388398 | |
| lytic potency against various mycobacterial strains of the phage isolated from mycobacterium leprae murium "douglas". | the lytic potency of a newly isolated phage al-1 obtained from the laboratory strain m. leprae murium "douglas" was examined. the phage was multiplied on the laboratory strain m. smegmatis atcc 607 and for the lytic test 0.1 ml of suspension containing pfu 10(5) was used. in the whole 18 mycobacterial strains both slowly and fast growing multiplied in liquid sula's medium were tested. for phage lytic tests two simple agar media and standard redmond's medium rva-24 were used. the examined slowly ... | 1984 | 6389043 |
| isolation of mycobacterial phage from the laboratory strain mycobacterium leprae murium "douglas". | the colony microstructure of the laboratory strain mycobacterium leprae murium "douglas" cultivated on ogawa's egg medium was examined. a bioptical sample from the liver of a white mouse subcutaneously infected and observed for ten months was used as inoculum. the inoculum contained 5.2 x 10(9) acidfast rods. the ogawa's media were incubated in 5% atmosphere of co2 at 33 degrees c to 37 degrees c for 6 to 10 months. the outgrown colonies were killed with a formol solution, then embedded into the ... | 1984 | 6389044 |
| phenolic glycolipid 1 of mycobacterium leprae causes nonspecific inflammation but has no effect on cell-mediated responses in mice. | the involvement of the phenolic glycolipid from mycobacterium leprae in cell-mediated immunity has been investigated in this study. the phenolic glycolipid itself does not appear to stimulate cell-mediated immunity directly, as shown by its failure to elicit a classical delayed-type hypersensitivity response in mice immunized with m. leprae or to stimulate m. leprae-immune lymph node cells in a lymphoproliferative assay. intradermal vaccination with the phenolic glycolipid failed to influence th ... | 1984 | 6389362 |
| the placenta in leprosy. | eighty-one placentae from women with leprosy and 17 placentae from healthy controls were subjected to a detailed macroscopic, light microscopic, ultrastructural, immunopathological, microbiological and biochemical study. the placental morphology and immunohistology were normal, and there was no morphological evidence of infection of the placenta due to m. leprae. no acid-fast bacilli or acid-fast bacillary granules were seen on light microscopy of any of the placentae from leprous women, althoug ... | 1984 | 6390420 |
| [possible mechanisms of cellular degeneration in macrophages infected with bcg in vitro and in leprous granulomas]. | 1984 | 6390584 | |
| evidence for the presence of m. leprae reactive t lymphocytes in patients with lepromatous leprosy. | evidence for the presence of mycobacterium leprae reactive t cells in many lepromatous leprosy (ll) patients was obtained using in vitro antigen-induced lymphoproliferative responses. (1) co-cultures of t enriched cells from ll patients when combined with 2 h adherent cells (ac) from hla-d compatible tuberculoid leprosy individuals showed significant levels of 3h-thymidine incorporation in the presence of soluble and integral m. leprae antigens. (2) more interestingly, autologous t cell + ac co- ... | 1984 | 6391762 |
| a dual effect of tilorone on multiplication of mycobacterium leprae in mice. | tilorone, a synthetic inducer of interferon found earlier to inhibit multiplication of mycobacterium leprae in the foot pad of the mouse while it enhanced infections of mice by m. lepraemurium and m. marinum, has been shown to exert a dual effect on m. leprae infection of the mouse. when administered continuously, incorporated into the mouse diet in a concentration of 0.015 g per 100 g diet, the drug was usually immunosuppressive, permitting enhanced multiplication of the organisms. when adminis ... | 1984 | 6395678 |
| phosphatidylinositomannosides in lepromatous leprosy nodules. | 1984 | 6396226 | |
| dapsone resistance in patients attending central leprosy teaching and research institute, chengalpattu (south india). | the central leprosy teaching and research institute (c.l.t. & r.i.) chengalpattu, took up studies on dapsone resistance in m. leprae from 1974. from 1978, the study was further strengthened by a project under thelep (tdr) for eliciting information on the efficacy of certain drug regimens. the thelep studies were to be conducted only on the dapsone sensitive untreated cases and, therefore, directed towards the detection of primary resistance, while the non-thelep institutional studies were concen ... | 1984 | 6396340 |
| the sensitivity and specificity of fluorescent leprosy antibody absorption (fla-abs) test for detecting subclinical infection with mycobacterium leprae. | 1984 | 6396473 | |
| anti-mycobacterium leprae antibodies induced by lepromin injection as demonstrated by indirect immunofluorescence. | 1984 | 6396474 | |
| the bactericidal activity of various aminoglycoside antibiotics against mycobacterium leprae in mice. | 1984 | 6396475 | |
| prevalence of secondary dapsone-resistant leprosy in upper volta. | 1984 | 6396476 | |
| leprosy immunology--some aspects of the role of the immune system in the pathogenesis of disease. | 1984 | 6396478 | |
| untreated hansen's disease of the eye: a clinicopathological report. | a maltese immigrant presented with intermittent bilateral anterior uveitis for which no cause could be found. the inflammation did not respond to topical treatment and ultimately the left eye developed a hypopyon and was enucleated. histological examination revealed granulomatous inflammation and large numbers of mycobacterium leprae throughout the anterior segment. occasional foci of inflammation containing mycobacterium leprae were found in the vitreous extending to the retina at the posterior ... | 1984 | 6397175 |
| the early serodiagnosis of leprosy. i. the use of counterimmunoelectrophoresis and enzyme-linked immunosorbent assay. | 1984 | 6397799 | |
| leprosy--in pursuit of a vaccine. | clinical leprosy is characterized by varying manifestations between tuberculoid and lepromatous leprosy. in the former state the patient is able to elicit a cell-mediated immune response whereas in the latter, there is usually a humoral response. an understanding of this immunological balance is crucial in the search for a vaccine which will control the disease. the development of a possible anti-mycobacterium leprae vaccine has been advanced by the isolation of organisms from the infected tissu ... | 1984 | 6397930 |
| monoclonal antibodies against mycobacterium leprae and their applications in leprosy research. | 1984 | 6398337 | |
| trophic changes and extensive dissemination in normal mice infected with human mycobacterium leprae. | closely bred swiss albino normal mice (lecca strain) were inoculated in the footpad with m. leprae, at room temperature. the animals were harvested at 3, 6 and 9 months post inoculation, and bacillary counts were made. trophic changes were observed in the tail-tips, ears, footpads and forepaws 12-14 months post inoculation in a group which was allowed to survive. the histopathological changes and bacillary infiltration was found in many tissues/organs. the possibility of studying this normal str ... | 1984 | 6398338 |
| induction of delayed-type hypersensitivity by icrc anti-leprosy vaccine and the adoptive transfer of cell-mediated immunity in mice. | the immunogenic potency of anti-leprosy icrc vaccine preparations was determined by foot pad enlargement in mice. female balb/c mice were sensitized by i.d. route with irradiated, heat-killed or live antigens of 2 strains (c-44 and c-75) of icrc bacilli and tested against lepromin and icrc-antigens. icrc antigens sensitized mice against lepromin; similarly, bcg and m. leprae sensitized mice, which showed good cross reactivity with icrc antigens. a mixture of irradiated icrc bacilli and live bcg ... | 1984 | 6398339 |
| hydrolytic enzymes in macrophages from leprosy patients in presence of mycobacterium leprae. | presence of mycobacterium leprae in association with in vitro cultured macrophages, from bacillary negative long term treated lepromatous leprosy patients, induces reduced level of protein and lowering of hydrolytic enzymes like p-glucuronidase, lysozyme and lactic dehydrogenase. alkaline phosphatase, on the other hand is increased. in the macrophages from normal healthy individuals or tuberculoid leprosy patients, presence of m.leprae increases both protein and levels of all the above enzymes. ... | 1984 | 6398340 |
| extended studies on subclinical infection in leprosy. | 1984 | 6398341 | |
| central nervous system involvement in leprosy. | 1984 | 6398342 | |
| primary dapsone resistant leprosy in nepal. | mouse foot pad testing for primary dapsone resistant leprosy was performed in 15 patients. 13 were resistant (87%). two patients below 10 years were living with lepromatous leprosy mothers, one male aged 21, was born in kokana leprosarium and ten others gave no history of known contact of leprosy. | 1984 | 6398343 |
| prevalence of secondary dapsone resistance in nepal. | between 1980-1982, 56 patients suspected of developing dapsone resistant leprosy were seen at the skin clinic of anandaban leprosy hospital and kokana leprosarium. out of 56, 16 control mice had no growth. 29 patients (72.5%) were proved resistant by mouse foot pad tests. | 1984 | 6398344 |
| role of dapsone in chemotherapy of leprosy--a comparison of responses to therapy in two cohorts in 1960s and 1970s. | two cohorts of bacterio-positive cases registered and started on treatment during 1960-62 and 1968-70 were studied for differences in their response to dds monotherapy. proportion of male and female cases, mean bacteriological index (b.i.) and the distribution of cases according to the initial bi did not differ between the two cohorts. 49.7% of the patients in the ii cohort were taking regular treatment against 27.4% in the i cohort. at the end of 7 years of treatment, proportion of the cases th ... | 1984 | 6398345 |
| effects of certain ayurvedic preparations on the multiplication of m. leprae in mouse foot-pads. | 1984 | 6398346 | |
| characterization of mycobacterium leprae by lipid analysis. | the lipid composition of the leprosy bacillus, harvested from experimentally infected nine-banded armadillos, strongly supports it status as a distinct species of the genus mycobacterium. phthiocerol dimycocerosate waxes and glycosylated phenophthiocerol dimycocerosates are distinct from those characterised from a number of other mycobacteria. the polar lipids of a single isolate lack diacylated forms of phosphatidylinositol di- and pentamannosides, lipids usually found in most mycobacteria. a s ... | 1984 | 6398576 |
| lipids as taxonomic markers for bacteria derived from leprosy infections. | lipid analysis allows the specific detection of m. leprae among various other bacteria isolated from leprosy lesions. in this report mycolates and glycolipid compositions were used for such a discrimination. comparative studies of the lipid composition of tissue fragments from different organs of experimentally infected armadillos, and of cultivable strains isolated from these tissues showed that the last ones did not multiply extensively in the tissues of the animals. | 1984 | 6398577 |
| dna from mycobacterium leprae. | the cell walls of mycobacteria resisting all enzymatic and chemical methods for solubilization, good quality dna require that they are converted into spheroplasts before extraction. mycobacterium leprae cannot grow in laboratory media, spheroplasts cannot be induced, and therefore the bacteria must be ruptured using physical methods. in these investigations m. leprae was disrupted by sonication. the dna isolated from sonicated m. smegmatis and sonicated purified dna isolated from m. smegmatis sp ... | 1984 | 6398578 |
| classification and identification of mycobacterium leprae. | 1984 | 6398579 | |
| propionibacterium, corynebacterium, mycobacterium and lepra bacilli. | evidence is presented which suggests that certain key markers of lepra bacilli reside collectively in proprionibacterium acnes, corynebacterium tuberculostearicum and mycobacterium leprae. the unrestricted replication of mycobacterium leprae depends most probably upon the presence of an immune-deficiency-inducing viral agent or possibly on the combined effects of the organisms considered. | 1984 | 6398580 |
| liberated intracellular pathogen--leprosy model. | thirty-three mycobacterial strains, 30 by culture and 3 directly from tissues, isolated from lepromatous leprosy and leprosy infected armadillos, were compared by numerial taxonomy and by antibodies from lepromatous patients. an additional 17 strains of the m-a-i-s complex were similarly compared and all strains were compared by rabbit antibodies induced by tissue bacilli from armadillos from culture hz-15 and by members of the m-a-i-s complex. the results are discussed in terms of the identific ... | 1984 | 6398581 |
| macrophage interaction with mycobacteria including m. leprae. | resistance properties of pathogenic mycobacteria to macrophage bactericidal activity seems to be due mostly to the composition and constitution of their cell walls. in the case of mycobacterium tuberculosis, sulfatides and polyglutamic acid could be implicated in the phenomenon of fusion inhibition between phagosomes and lysosomes. m. leprae and m. lepraemurium, which do not seem to inhibit fusions are protected by a thick electron transparent zone (etz) that seems to be composed of mycosides. t ... | 1984 | 6398582 |
| cytochemical characterization of mycobacterial outer surfaces. | a cytochemical study of mycobacterial outer surfaces was carried out on both pathogenic (m. leprae, m. avium) and non pathogenic (m. aurum) strains. different cytochemical markers were used: ruthenium red, concanavalin a, wheat germ agglutinin, colloidal iron and cationized ferritin. the cytochemical staining pattern varied according to the species studied. the relationship between outer surface properties of mycobacteria and their capacity of adhesion to or ingestion by bone marrow macrophages ... | 1984 | 6398583 |
| freeze-etching and freeze-fracture structural features of cell envelopes in mycobacteria and leprosy derived corynebacteria. | the structural properties of the cell wall and cell membrane of several mycobacteria and of leprosy derived corynebacteria are investigated by freeze-etching and freeze-fracture. in all cases the freeze-fracture split the cell wall in two asymmetric halves. the cell wall fracture faces of the mycobacteria are characterized by a filamentous network which vary with respect to the amount and complexity among microorganism of the same species and even more of different species. in ldc the structure ... | 1984 | 6398584 |
| macrophage activity in mycobacterium leprae infection. | the outcome of an m. leprae infection is likely to depend upon the balance between the invading organism and the host's immune response. macrophages are known to play a major role in this response and because m. leprae is an intracellular parasite, being found commonly in the macrophages of infected hosts, we have attempted to examine the macrophage/m. leprae relationship. our model has been the athymic nude mouse which has been shown to be susceptible to lepromatous infection but whose macropha ... | 1984 | 6398585 |
| phagocytosis of mycobacterium leprae and m. avium by armadillo lung fibroblasts and kidney epithelial cells. | in vitro cell cultures of lung fibroblasts and kidney epithelial cells were established from a freshly killed armadillo and were inoculated with mycobacterium leprae. lung fibroblasts were also inoculated with m. avium. phagocytosis was allowed for 6 h at an input of about 50 bacilli/cell, and the ultrastructure was then studied at 24 and 48 hours, and 4, 7 and 10 days. following observations were made: 1. armadillo cells could be maintained for nearly 3 months. 2. both lung fibroblasts and kidn ... | 1984 | 6398586 |
| growth characteristics of mycobacterium leprae. | 1984 | 6398587 | |
| correlation viability/morphology in mycobacterium leprae. | the present study regards the correlation between the percent of viable m. leprae (as determined by the mouse foot pad technique) and the quantitative ultrastructural analysis of m. leprae cells in 6 armadillo's samples and 1 nude mouse foot pad. the quantitative ultrastructural study of 3 ll patients and 1 m. leprae-infected nude mouse was correlated to the morphological index. the results show that most m. leprae cells with continuous undeformed cell walls, continuous symmetric membranes, ribo ... | 1984 | 6398588 |
| cultivation of m. leprae. | a peculiar yeast-like microorganism, isolated from leprous lesions, was discovered to produce the growth promoting factor for m. leprae. from the mass culture of this organism, the oil substance stimulating m. leprae growth was extracted with hot acid ethanol and purified using organic solvents. the final product was considered to be a kind of siderophore. when inoculating m. leprae on the special solid medium, contained chemically defined materials and supplemented with the growth factor, and i ... | 1984 | 6398589 |
| a comparative study of four rodent systems to monitor initial therapy of lepromatous leprosy: in search of a more sensitive system to assess bacterial viability. | 1984 | 6398591 | |
| experimental leprosy in the armadillo and nude mice: comparative histobacteriology and ultrastructure. | in last 14 years, armadillo has proved an ideal animal model for studying experimental leprosy and mass production of mycobacterium leprae. however recently a number of groups working with nude mice have claimed its ability as a better experimental model as far as leprosy research and production of leprosy bacilli is concerned. we therefore decided to compare experimental m. leprae infection of both armadillo and nude mice. we compared the degree of infection as well as the physiological and mor ... | 1984 | 6398592 |
| occurrence of antigen bcg 60 in leprosy derived corynebacteria and other coryneforms. | previous studies have shown that anti bcg 60 monoclonal antibody could recognize major antigen a 7 of m. leprae. in the present investigation we attempted to search the presence of the same antigen from the strains of leprosy derived corynebacteria (ldc+), which were isolated from leprosy lesions and were supposed to be the cultivable form of the leprosy bacillus. a comparative study was equally performed on 7 strains of corynebacteria which were isolated in france and had no relation with lepro ... | 1984 | 6398594 |