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immunological and structural relatedness of catabolic ornithine carbamoyltransferases and the anabolic enzymes of enterobacteria.purified catabolic ornithine carbamoyltransferase of pseudomonas putida and anabolic ornithine carbamoyltransferase (argf product) of escherichia coli k-12 were used to prepare antisera. the two specific antisera gave heterologous cross-reactions of various intensities with bacterial catabolic ornithine carbamoyltransferases formed by pseudomonas and representative organisms of other bacterial genera. the immunological cross-reactivity observed only between the catabolic ornithine carbamoyltrans ...19853968036
invasion of hep-2 cells by fecal isolates of aeromonas hydrophila.human diarrheal isolates of aeromonas hydrophila were found to be invasive in hep-2 cell monolayers. microscopic examination of two isolates from patients with symptoms of dysentery revealed that infected hep-2 cells contained up to 50 bacteria localized within areas of the cytoplasm. a quantitative invasion assay was developed, using viable counts of total intracellular bacteria. five fecal isolates of a. hydrophila were classified as invasive, with an average of 0.1 up to 2 bacteria per hep-2 ...19853972447
protein export by a gram-negative bacterium: production of aerolysin by aeromonas hydrophila.the synthesis and export of aerolysin, an extracellular protein toxin released by the gram-negative bacterium aeromonas hydrophila, was studied by pulse-labeling with [35s]methionine. the toxin was synthesized as a higher-molecular-weight precursor. this was processed cotranslationally, resulting in the appearance within the cell of the mature protein, which was then exported to the supernatant. precursor aerolysin accumulated in cells incubated in the presence of carbonyl cyanide m-chlorophenyl ...19853972770
pathogenicity of aeromonas.the ability of 15 aeromonas sobria and 9 aeromonas hydrophila isolates to cause subcutaneous lesions was tested. an inoculum of 10(11) colony forming units/l was injected subcutaneously into mice. surviving animals developed a subcutaneous abscess and/or localised skin sloughing and loss of hair (alopecia). an abscess was induced by all nine a. hydrophila isolates and by three of the 15 a. sobria isolates. the induction of local epidermal sloughing and loss of hair followed challenge with either ...19853981022
methods for the detection of haemagglutinins in aeromonas.when grown in specified conditions and tested by a rocked-tile method, 40 of 41 isolates of two species of aeromonas formed simultaneously at least two haemagglutinins among which were: (i) a mannose-sensitive haemagglutinins with strongest activity for guinea-pig or fowl red cells, formed by all of 31 isolates of a. hydrophila and 9 of 10 isolates of a. punctata ss. caviae; (ii) a haemagglutinin, sensitive to l-fucose or d-mannose, that reacted with human red cells and which was formed by all 4 ...19853981614
aeromonas hydrophila--an accepted enterotoxigenic "neoenteropathogen"? 19853989329
biochemical and immunological characterization of the cell surface of the fish pathogenic bacterium aeromonas salmonicida.the identification of lipopolysaccharide as periodic acid-schiff positive material, present in the membrane fraction of the fish pathogenic gram-negative bacterium aeromonas salmonicida, analyzed by sds-polyacrylamide gel electrophoresis, is shown. such analysis has revealed several periodic acid-schiff positive bands and many membrane proteins among which a pathogenicity-related mr 54000 protein as a constituent of an additional surface layer outside the outer membrane (evenberg et al., (1982) ...19853995026
humoral response and memory formation in carp after injection of aeromonas hydrophila bacterin.the humoral immune response of carp (cyprinus carpio) upon a bacterial fish pathogen aeromonas hydrophila was studied in relation to memory formation. after a single intramuscular (i.m.) injection of formalin killed a. hydrophila cells (f-ah), maximum serum ab (ab) titers were observed at day 20. distinct titers were still seen at day 360 in the groups injected with a medium or high antigen (ag) dose (10(7) respectively 10(9) f-ah). the effect of a second immunization with a high ag dose was stu ...19853996709
[spontaneous aeromonas hydrophila peritonitis in the cirrhotic. apropos of 2 cases]. 19854001643
comparative in vitro activities of selected antimicrobial agents against aeromonas species and plesiomonas shigelloides.the in vitro antimicrobial susceptibilities of fecal isolates of aeromonas caviae, aeromonas hydrophila, aeromonas sobria, and plesiomonas shigelloides were tested by agar dilution. this in vitro study suggested that ciprofloxacin, enoxacin, norfloxacin, tetracycline, and trimethoprim-sulfamethoxazole might be effective oral agents for treatment of diarrhea involving aeromonas spp. or p. shigelloides.19854004196
clinical and microbiological features of aeromonas hydrophila-associated diarrhea.the prevalence of aeromonas hydrophila in stool specimens from patients with diarrhea was studied during 18 months. a. hydrophila was found in 1.1% of patients with diarrhea and in none of 533 control patients (p less than 0.02). cases were detected 1.5 times more often during the summer months than the winter months, and most occurred in children less than 2 years of age. clinical features included fever greater than 38 degrees c (55%), abdominal cramps (35%), vomiting (25%), and duration of il ...19854008621
rapid microbiochemical method for presumptive identification of gastroenteritis-associated members of the family enterobacteriaceae.a method for rapid screening of isolates of pathogenic members of the family enterobacteriaceae is described. flow charts are used in conjunction with triple sugar iron agar, o-nitrophenyl-beta-d-galactopyranoside-phenylalanine-motility sulfate screening media, oxidase test, and six rapid biochemical tests, namely, lysine decarboxylase, urease, indole, esculin hydrolysis, malonate, and xylose. this scheme is used to provide an inexpensive but rapid presumptive identification of salmonella, shige ...19854008622
comparison of the cathra repliscan ii, the automicrobic system gram-negative general susceptibility-plus card, and the micro-media system fox panel for dilution susceptibility testing of gram-negative bacilli.a comparative evaluation was done to test the accuracy of the cathra repliscan ii agar dilution system (diagnostic equipment, inc., st. paul, minn.), the automicrobic system with gram-negative general susceptibility-plus card (vitek systems, inc., hazelwood, mo.), and the micro-media fox panel micro broth dilution system (micro-media systems, inc., san jose, calif.) in determining mics of 12 antibiotics for 200 gram-negative bacilli. of the 200 strains tested, 12 isolates did not grow in one of ...19854008625
[gram-negative nonfermenting bacteria in respiratory tract infections]. 19854010380
isolation of aeromonas species from animals. 19854018073
invasiveness of aeromonas spp. in relation to biotype, virulence factors, and clinical features.of 69 fecal isolates of aeromonas spp., 18 had the ability to invade hep-2 cells. invasiveness correlated with biotype; of the 18 invasive strains, 16 were a. sobria and 2 were a. hydrophila. no invasive strains were found among the a. caviae. of the 18 invasive strains, 13 were enterotoxigenic. of the enterotoxigenic and invasive strains, 12 were a. sobria, but enterotoxicity was also more common among noninvasive strains of a. sobria. fucose-resistant hemagglutination was also more common in a ...19854019741
the antagonism of tetracycline and ferric iron in vivo.to test the hypothesis that the in-vivo antibiotic action of tetracycline might be affected by ferric iron and the enhancement of infection by ferric iron by tetracycline, the actions of intraperitoneal antibiotic and local ferric ammonium citrate, given separately and together, were measured in the dorsal skin of guinea-pigs bearing lesions due to staphylococci, streptococci, a proteus sp., an erysipelothrix sp., clostridium perfringens, pseudomonas aeruginosa, aeromonas hydrophila and klebsiel ...19854020849
bacteremia caused by aeromonas species in hospitalized cancer patients.bacteremia caused by aeromonas species occurred in 24 hospitalized patients in a cancer institute during a 13-year period. all but one of these patients had a malignancy (88% had leukemia), and most were receiving chemotherapy for cancer. there was a striking numerical predominance of male patients (82%). unlike some previously described patients with infections due to this organism, none of these 24 patients had recently been exposed to fresh or salt water or to fish. the source of the infectin ...19854023526
a transition-state-analog inhibitor influences zinc-binding by aeromonas aminopeptidase.the transition-state-analog inhibitor, 1-butaneboronic acid, markedly enhances the uptake of one g-atom of zn2+ ions from a metal ion buffer system by zn-depleted aeromonas aminopeptidase. in contrast, a substrate-analog inhibitor, n-valeramide, does not perturb the equilibrium between zn2+ ions and the enzyme in a metal ion buffer system. these results establish a role for metal ions in the binding of 1-butaneboronic acid to aeromonas amino-peptidase and strongly imply that a bound zn2+ ion int ...19854026862
salmonellae and pollution indicator bacteria in municipal and food processing effluents and the cornwallis river.a study was conducted to determine the incidence of fecal coliforms, fecal streptococci, aeromonas hydrophilia, and salmonella spp. in the waste discharges of seven sewage treatment plants, four fruit and vegetable canneries, a meat packing plant, a poultry processing plant, and a potato processing plant located along the cornwallis river in nova scotia, canada. surface water samples were also collected from 13 locations in the river to assess the impact of these waste discharges on the receivin ...19854027779
bacteria in bivalve shellfish with special reference to the oyster.the bacterial flora of the pacific oyster crassostrea gigas, the sea mussel perna viridis and the arkshell clam scapharca cornea differed considerably from that of seawater in both numbers and generic composition. the numbers of heterotrophic bacteria in the bivalve shellfish, including the anaerobes and spore-forming bacteria, were greater than that in the surrounding water. pseudomonas spp. were the dominant organisms, comprising over one third of the 321 strains characterized after isolation ...19854030530
[amino acid composition of white poultry meat contaminated with psychrophilic microorganisms].studied was the effect of psychrophilic organisms of the acinetobacter, aeromonas, alcaligenes, and flavobacter genera on the amino acid composition of white poultry meat stored at 0 degrees to 4 degrees c in the course of 48 hours, and at 18 degrees c in the course of 6 to 12 months. the quantitative determination of individual amino acids was carried out by means of an automatic amino acid analyzer, hitachi type, after the method of speckman, stein, and moore. no changes were established in th ...19854035999
in vitro susceptibilities of aeromonas hydrophila, aeromonas sobria, and aeromonas caviae to 22 antimicrobial agents.mics of 22 antimicrobial agents for 60 strains of three aeromonas species were determined by a microdilution method. the newer cephalosporins such as moxalactam, cefotaxime, and cefoperazone, the aminoglycosides, and chloramphenicol, tetracycline, nitrofurantoin, and trimethoprim-sulfamethoxazole inhibited most of the strains studied. within the genus, a. hydrophila was more resistant than either a. caviae or a. sobria to the antibiotics tested.19854037775
immunity to aeromonas salmonicida in coho salmon (oncorhynchus kisutch) induced by modified freund's complete adjuvant: its non-specific nature and the probable role of macrophages in the phenomenon.juvenile coho salmon (oncorhynchus kisutch), vaccinated with one intraperitoneal injection of formalin-killed virulent aeromonas salmonicida cells suspended in saline, showed increased protection against approximately one ld60 of homologous challenge administered at 30 days post-vaccination. under similar conditions, coho vaccinated with a modified complete freund's adjuvant (mfca) alone were also equally protected. when measured against a more severe a. salmonicida challenge of approximately on ...19854043481
demonstration of cholera toxin-related factor in cultures of aeromonas species by enzyme-linked immunosorbent assay.the production of toxins by aeromonas species was examined by the suckling mouse test, the hemolysin test, and the enzyme-linked immunosorbent assay with anticholera enterotoxin. a factor that was immunologically related to cholera enterotoxin was produced by 5 of 14 strains of aeromonas hydrophila and 4 of 15 strains of aeromonas sobria. analysis by these assays and by a test for heat stability suggested that the factor differed from hemolysin and from toxin that was active in the suckling mous ...19854044041
lack of correlation between known virulence properties of aeromonas hydrophila and enteropathogenicity for humans.five strains of aeromonas hydrophila were selected for use in volunteer challenge trials. all five strains produced cytotoxin, hemolysin enterotoxin, lysine decarboxylase, acetylmethylcarbinol, and dnase. two strains hydrolyzed esculin. all strains produced purulent hemorrhagic fluid accumulation in rabbit ileal loops, but failed to induce keratoconjunctivitis in guinea pigs. none of the strains produced mannose-resistant hemagglutinins. in challenge studies, diarrhea was demonstrated in only 2 ...19854044042
the purification and some properties of h-lysin from aeromonas salmonicida.h-lysin from aeromonas salmonicida has been purified 1770-fold by freeze fractionation, ammonium sulphate precipitation, ion exchange chromatography and gel filtration chromatography. the purified material was predominantly h-lysin, devoid of detectable t-lysin, caseinase or gelatinase activity, although glycerophospholipid: cholesterol acyltransferase (gcat) activity was present. the results suggested that h-lysin and gcat activities were due to different extracellular products. studies of the ...19854045422
clinical predictors of acute bacterial diarrhea in young children.this prospective study assessed the value of presenting history, physical examination, and screening laboratory tests in predicting whether diarrhea in a young child is associated with a stool culture positive for a bacterial pathogen. acutely ill children less than 4 years old were studied in a hospital outpatient setting. two hundred patients were seen in a 9 1/2-month period, which encompassed the seasons of summer, fall, and winter. one hundred ninety-five patients had cultures completed and ...19854047797
[metabolic traits of plesiomonas shigelloides strains isolated from children with acute enteritis]. 19854048753
unfamiliar bacteria isolated from cats. 19854049692
comparison of api 20e, api rapid e, and api rapid nft for identification of members of the family vibrionaceae.sixty isolates, from nine species of the family vibrionaceae, were tested by the api 20e, api rapid e, and api rapid nft systems. results were compared with those obtained with standard biochemicals. included were 7 aeromonas caviae isolates, 27 aeromonas hydrophilia isolates, 10 aeromonas sobria isolates, 3 plesiomonas shigelloides isolates, 3 vibrio alginolyticus isolates, 3 vibrio cholerae isolates, 1 vibrio fluvialis isolate, 5 vibrio parahaemolyticus isolates, and 1 vibrio vulnificus isolat ...19854056003
cephalothin susceptibility as a potential marker for the aeromonas sobria group.fifty-four motile aeromonas strains, composing the three currently recognizable species, were tested for susceptibility to cephalothin by broth dilution and disk agar diffusion assays. cephalothin susceptibility was significantly associated with aeromonas sobria (p less than 0.001) and may be an additional phenotypic marker useful in the identification of this species.19854056009
media for the isolation of aeromonas hydrophila.isolation rates of aeromonas hydrophila from stool samples of symptomatic and asymptomatic individuals were examined for several common enteric media. sheep blood agar with 10 micrograms of ampicillin per ml, preceded by overnight enrichment in alkaline peptone water, yielded 2.6 times the number of isolates as the other media examined and is recommended for the isolation of a. hydrophila from humans.19854056015
recurrent aeromonas hydrophila septicemia, a case report. 19854056647
[aeromonas hydrophila bacteremia. clinical and bacteriological characteristics. apropos of 16 cases]. 19854059610
antibacterial activity of amifloxacin (win 49, 375), a new quinolone agent.the in vitro activity of amifloxacin, a quinolone antimicrobial agent was compared with those of ciprofloxacin, enoxacin, ofloxacin and norfloxacin against gram-positive and gram-negative bacteria. ninety percent of escherichia coli, klebsiella species, aeromonas, salmonella, shigella, citrobacter, enterobacter species, proteus mirabilis, serratia marcescens, and morganella morganii were inhibited by less than or equal to 0.5 microgram/ml. amifloxacin inhibited branhamella, haemophilus, and neis ...19854064609
aeromonas caviae: an enteric pathogen?of the aeromonas spp. isolated routinely in our laboratory from human feces, about two thirds represent aeromonas caviae. in contrast to aeromonas hydrophila and aeromonas sobria, this species has been considered to be of little enteropathogenic significance due to the absence of known virulence factors. the clinical data presented here suggest a clinical significance of a. caviae, at least in some cases. as yet nothing is known about the pathogenic mechanisms involved.19854066046
peritrichous flagella in mesophilic strains of aeromonas.a total of 186 mesophilic strains of aeromonas, comprising 151 a. hydrophila and 35 a. caviae, were tested for peritrichous flagella by leifson's staining method. when incubated on solid medium for 18 hr at 22 c, peritrichous flagella were demonstrated in 28 (18.5%) of the 151 strains of a. hydrophila and in 6 (17.1%) of the 35 strains of a. caviae. the peritrichous flagella in these 34 mesophilic strains of aeromonas were also observed by electron microscopy.19854068347
ticarcillin plus clavulanic acid versus moxalactam therapy of osteomyelitis, septic arthritis, and skin and soft tissue infections.a controlled, randomized study to compare the efficacy and safety of ticarcillin plus clavulanic acid with moxalactam was carried out in 25 evaluable patients with bone, joint, and skin or skin structure infections. of the 13 patients in the ticarcillin plus clavulanic acid-treated group, nine had osteomyelitis, two had septic arthritis, one had cellulitis, and one had a wound infection. four of the 12 moxalactam-treated patients had osteomyelitis, one had septic arthritis, and the other seven h ...19854073082
aeromonas-related diarrhea in adults.we have reviewed the incidence of aeromonas in patients with enteric disease at our hospital and found it to be the highest of any potential enteric pathogen. eighty adult patients with diarrhea had aeromonas isolated from feces, and in 73 aeromonas was the only potential bacterial or parasitic pathogen detected. the spectrum of illness in patients with aeromonas-related diarrhea ranged from acute, self-limited diarrhea to a chronic, indolent diarrheal illness. sixteen percent (13/80) of the pat ...19854074035
prevalence and pathogenicity of aeromonas hydrophila.a field survey to determine the prevalence of aeromonas hydrophila revealed a recovery rate of 8% in 141 specimens derived from a range of live, companion, and exotic avian species. the prevalence rate was similar in 240 sequential postmortem submissions during 1984. studies on the pathogenicity of a. hydrophila showed that 2-to-4-day-old chicks and turkey poults were highly susceptible to exposure via the subcutaneous, yolk-sac, and intracerebral routes, and mortality of 80-100% occurred within ...19854074237
spectral and kinetic studies of metal-substituted aeromonas aminopeptidase: nonidentical, interacting metal-binding sites.apoenzyme prepared by removal of the 2 mol of zn2+/mol from aeromonas aminopeptidase is inactive. addition of zn2+ reactivates it completely, and reconstitution with co2+, ni2+, or cu2+ results in a 5.0-, 9.8-, and 10-fold more active enzyme than native aminopeptidase, respectively. equilibrium dialysis and spectral titration experiments with co2+ confirm the stoichiometry of 2 mol of metal/mol. the addition of only 1 mol of metal/mol completely restores activity characteristic of the particular ...19854074699
antigen localization in the lymphoid organs of carp (cyprinus carpio).a brief morphological description is given of the spleen, head kidney and trunk kidney, the three peripheral lymphoid organs of carp. an antigen-localization study was carried out using aeromonas hydrophila (a cellular bacterial antigen) and an indirect immunofluorescence test. examination of the lymphoid organs at various times after injection (up to 12 months) showed that antigen was at first present in splenic ellipsoids and in solitary phagocytic cells in the spleen, head and trunk kidney. l ...19854075373
an unusual epizootic of anuran aeromoniasis. 19854077645
growth of aeromonas spp. on cefsulodin-irgasan-novobiocin agar selective for yersinia enterocolitica.twenty-eight strains of aeromonas spp. were analyzed for their ability to grow on two different kinds of cefsulodin-irgasan (triclosan; ciba-geigy ag, basel, switzerland)-novobiocin (cin) agar containing 15 or 4 mg of cefsulodin per ml and on inositol-bile salts-brilliant green (ibb) agar. relative to blood agar, 68% of the strains were inhibited by more than 2 logs (i.e., less than 1% growth) at 37 degrees c (39% at 25 degrees c) on cin i (high cefsulodin concentration), 7% were inhibited at ei ...19854077959
holding effects on coliform enumeration in drinking water samples.standard procedures for analyzing drinking water stress the need to adhere to the time and temperature conditions recommended for holding samples collected for microbiological testing. the national drinking water laboratory certification program requires compliance with these holding limits, but some investigators have reported difficulties in meeting them. research was conducted by standard analytical methods to determine if changes occurred when samples were held at 5 and 22 degrees c and anal ...19854083877
[isolation of aeromonas hydrophila in diarrhea. characterization of enterotoxinogenic strains and clinical relations].aeromonas hydrophila is isolated from diarrhoea specimens with increasing frequency. the interest in this organism at the present time is related to the fact that it can produce a number of toxins, in particular alpha and beta cytotoxic haemolysins, an enterotoxin and various enzymes. the authors determined the frequency of isolation of this organism and tested the haemolytic, cytotoxic and enterotoxic effects of culture filtrates in all of the stool specimens received in their laboratory over a ...19854091318
use of non-carbonated soft drinks to provide safe drinking water.non-carbonated, low-calorie soft drink concentrates (cordials), when diluted according to manufacturers' instructions, had significant antibacterial effects in vitro. bacteria affected include vibrio cholerae, aeromonas hydrophila, shigella sonnei, salmonella typhimurium and escherichia coli. with vibrios, bacterial counts were reduced from 10(6)/ml to undetectable numbers in less than 10 min. escherichia coli in an initial concentration of 10(6)/ml became undetectable after incubation for 1 h w ...19852409896
eosinophilic granular cells (egc) and histamine responses to aeromonas salmonicida toxins in rainbow trout.the function of eosinophilic granular cells (egcs) in salmonids is unknown. in a previous study of the pathogenesis of a. salmonicida, injection of crude exotoxins into rainbow trout were shown to reproduce the lesions associated with furunculosis and an accompanying lesion, the dispersion and degranulation of egcs in the intestinal wall was reported. the present study investigated this phenomenon in relationship to the histamine content of the gut. in fish injected i/p with a. salmonicida exoto ...19852410306
electrophoretic and immunochemical analyses of the lipopolysaccharides from various strains of aeromonas hydrophila.sodium dodecyl sulfate-polyacrylamide gel electrophoresis was used to analyze the lipopolysaccharides isolated from strains of aeromonas hydrophila which exhibit virulence for fish and which autoaggregate during growth in static broth culture. the lipopolysaccharides contained o-polysaccharide chains of homogeneous chain length. two of the strains produced a surface protein array, and immunofluorescence and phage-binding studies revealed that a number of these o-polysaccharide chains of homogene ...19852413008
the rate of evolutionary divergence of initiation factors if2 and if3 in various bacterial species determined quantitatively by immunoblotting.antibodies to escherichia coli translational initiation factors if2 and if3 were used for an immunological comparison of unpurified proteins from the following genera: salmonella, serratia, proteus, aeromonas, pseudomonas, streptococcus, sarcina and bacillus. immunological relatedness was compared by ouchterlony double diffusion experiments and immunoblotting analysis. immunoblotting is a quantitative technique for measuring levels of specific proteins in crude cell lysates. we have used this te ...19846084987
aeromonas hydrophila: an outbreak of hospital infection.aeromonas hydrophila is a gram-negative water-borne organism widely distributed in the environment. this organism is a recognized cause of diarrhoea and an opportunist pathogen in immunosuppressed patients. an outbreak of hospital-acquired infection which included three cases of pneumonia is reported. the organism was isolated from 19 patients.19846085098
pyogenic meningitis manifesting during therapy for aeromonas hydrophila sepsis.pyogenic meningitis became apparent on the third day of ampicillin and gentamicin therapy for aeromonas hydrophila sepsis in a patient with severe alcoholic hepatitis. the patient responded clinically to therapy with intravenous cefotaxime sodium and gentamicin sulfate. antibiotic therapy that provides adequate csf concentrations should be considered in the treatment of patients with aeromonas sepsis.19846091581
new medium for detection of esterase and gelatinase activity.a new medium was developed for detecting esterase and gelatinase activities in aerobic and facultatively anaerobic bacteria. the new medium was tested with various strains of bacteria and the results showed agreement between the reactions in the new medium and those obtained by conventional techniques. the new medium is more economical and may be used for a rapid differentiation of serratia, aeromonas and vibrio species from biochemically similar bacteria.19846098101
immunodominant protein epitopes. i. induction of suppression to hen egg white lysozyme is obliterated by removal of the first three n-terminal amino acids.the lack of response to hen egg white lysozyme (hel) by c57bl (h-2b) mice has been demonstrated previously to be related to the induction of suppressor t (ts) cells which recognize the amino terminal region of hel. in this report, the nature of the protein determinant required for ts cell induction is more precisely detailed using des-1,2,3-hel (ap-hel) prepared with an aminopeptidase purified from aeromonas proteolytica . remarkably, the removal of just these three amino acids obliterates the a ...19846202528
the use of a biotyping system to investigate an unusual clustering bacteraemias caused by aeromonas species.aeromonas spp. were isolated from blood cultures taken from four clinically bacteraemic patients over an 18 day period on four separate wards. a common source was suspected and extensive environmental sampling revealed two more ward isolates of aeromonas spp. a biotyping system was employed which distinguished the strains from each other and indicated that a common source was unlikely. this coincidental clustering occurred in the autumn, a period when isolates from water and faeces are normally ...19846205061
esterase electrophoresis: a new epidemiological screening test for aeromonas hydrophila hospital infection. 19846208255
[aeromonas hydrophila septicemia. epidemiologic aspects. 15 cases].fifteen cases of aeromonas hydrophila septicaemia, characterized by their frequent pulmonary lesions and the severity of their course, are reported. the delay observed between admission to hospital and first symptoms, together with the presence of anatomical lesions or physiological disturbances suggesting bacterial invasion by the intestinal route, have led the authors to postulate a nosocomial digestive contamination, probably from water, as suggested by the particular ecology of this micro-or ...19846232567
studies with enterotoxigenic microorganisms: effects of candidate antidiarrhoeals in experimental animals in vivo.chlorpromazine or aspirin alone, when given to rats parenterally, reduced intestinal fluid secretion induced by cell-free preparations of enterotoxigenic organisms including escherichia coli, aeromonas hydrophila, staphylococcus pyogenes, and salmonella typhimurium. a combination of chlorpromazine-aspirin given parenterally caused much more marked reduction of fluid secretion. indomethacin also had significant antisecretory effects against a range of bacterial enterotoxins, while loperamide was ...19846237185
induction of nonspecific immunosuppression in a primitive teleost, rainbow trout (salmo gairdneri).induction of immunological non-reactivity to goldfish red blood cells (gfrbc), sheep red blood cells (srbc) and to formalin-killed aeromonas salmonicida cells was obtained in yearling rainbow trout raised under laboratory aquarium conditions (at 10 degrees c). haemolysin and agglutinin titres were measured 1 to 4 weeks after immunization. while trout immunized with low and high doses of antigen by intramuscular (im) and intraperitoneal (ip) injections produced high antibody titres, administratio ...19846238855
in vitro activity of pefloxacin compared to that of quinolones and other antimicrobial agents.pefloxacin is a new methyl-4-piperazinyl quinolone. it had mic90 values of less than 0.01 to 0.8 micrograms/ml for the majority of escherichia coli, klebsiella, oxytoca, citrobacter, providencia, enterobacter cloacae, enterobacter aerogenes, morganella and proteus mirabilis. it inhibited ampicillin, cephalexin and nalidixic acid resistant isolates of these species. against pseudomonas the pefloxacin mic90 was 3.1 micrograms/ml. staphylococcus aureus had a mic50 of 0.4 micrograms/ml and a mic90 o ...19846241847
[severe aeromonas hydrophila infections. apropos of 2 cases].the authors describe two personal cases of severe aeromonas hydrophila infection. they review the symptomatology and treatment of such cases and insist on the unusual features in their patients.19846322344
[aeromonas hydrophila in ivory coast epidemiologic and ecologic aspects and its enteropathogenic role].a research based on the analysis of 479 coprocultures , allow the isolation of 22 strains of aeromonas hydrophila. the epidemiological aspects, the contamination processes through water, food... and the enteropathogenic role of these aeromonas are discussed. these bacteria seen responsible of digestive disturbances from simple gastric discomfort (nausea, light abdominal pain...) to the actual choleriform syndrome. the isolation of 7 strains from the environment (water from lagune , wells; lagune ...19846327100
[an aeromonas hydrophila choleriform syndrome. apropos of a case from the ivory coast].an observation of a case of choleriform syndrome of the aeromonas hydrophila subspecies anaerogenes biotype ii is reported. in a country dealing with a severe comma bacillus under endemo -sporadic from, the case report here could be wrongly diagnosed as cholera.19846327101
comparative study of the nature and biological activities of bacterial enterotoxins.it is apparent that there are considerable similarities between many of the enterotoxins produced by enteric pathogens. although the effect of most of these toxins is restricted to the intestine in vivo, many cells are also sensitive to intoxication in vitro. the resultant in-vitro biochemical changes may have no pathological significance but serve to underline the central role of cyclic nucleotides in cellular fluid regulation. the biological activity of these enterotoxins is the result of inte ...19846327986
biosynthesis of bacterial glycogen: characterization of adenosine diphosphate glucose synthetases from enterobacter hafniae and aeromonas hydrophila.enterobacter hafniae and aeromonas hydrophila adpglucose synthetases were purified approximately 39- and 61-fold, respectively, over the crude extract. both enzymes were heat stable at 60 degrees c in the presence of inorganic phosphate. the molecular weights of both enzymes were approximately 200,000 which are similar to other enteric adpglucose synthetases studied. based on kinetic results obtained from the partially purified enzymes, the e. hafniae enzyme is activated twofold by phospho-enolp ...19846331331
resistance of aeromonas hydrophila to beta-lactam antibiotics.the activity of some beta-lactam antibiotics upon 20 strains of aeromonas hydrophila and some properties of their beta-lactamases have been studied. high degree of resistance to benzylpenicillin and lower resistance to ampicillin and cephaloridine was observed. clavulanic acid showed the highest activity. when clavulanic acid was assayed at subinhibitory concentrations in association with ampicillin. mics of the latter decreased to two- to eight-fold. all strains produced inducible beta-lactamas ...19846335147
methods for the isolation of aeromonas hydrophila and plesiomonas shigelloides from faeces.two solid selective media, xylose deoxycholate citrate agar (xdca) and bile salts brilliant green agar (bbg) and an enrichment broth-alkaline peptone water, were evaluated for the isolation of aeromonas hydrophila and plesiomonas shigelloides. alkaline peptone water and xdca are useful for recovery of aeromonas but not plesiomonas, whereas bbg is satisfactory for both organisms.19846368683
structural and immunochemical homogeneity of aeromonas salmonicida lipopolysaccharide.sodium dodecyl sulfate-polyacrylamide gel electrophoresis was used to analyze the lipopolysaccharides of typical and atypical strains of the fish pathogen aeromonas salmonicida. 32p intrinsically radiolabeled lipopolysaccharide in sarcosinate-extracted outer membrane preparations, lipopolysaccharide stained by silver in proteinase k-digested outer membrane preparations and whole cell lysates, as well as purified lipopolysaccharide, displayed o-polysaccharide chains which were unusually homogeneo ...19846370955
[partial purification of the extracellular proteolytic system of aeromonas hydrophila lp50: comparative chromatographic and electrophoretic study].a complex extracellular proteolytic system was produced by aeromonas hydrophila lp50 on glucose- polypeptone medium. partial purification of this system was accomplished by ammonium sulphate precipitation, acetone precipitation, gel filtration on sephacryl- s200 and chromatography on deae-sephacel. every stage was controlled by electrophoresis. this proteolytic system was constituted of three aminopeptidase and two endopeptidase components.19846372579
neuraminidase production by vibrio cholerae o1 and other diarrheagenic bacteria.vibrio cholerae o1 strains belonging to both biotypes (classical and el tor) and both serotypes (ogawa and inaba) produced neuraminidase which was released rather than cell bound. classical strains made more neuraminidase than did el tor strains. about one-third of v. cholerae non-o1 strains and one-fourth of aeromonas hydrophila strains were neuraminidase positive. strains of enterotoxigenic escherichia coli, vibrio parahaemolyticus, and shigella spp. did not produce detectable neuraminidase.19846373618
aeromonas: biology of the organism and diseases in children. 19846374630
[pathogen distribution in waste water sprinkler irrigation].the spray irrigation with pretreated wastewater was investigated on the sewage farm of the braunschweig sewage utilization association. the emission of airborne bacteria was measured by means of andersen sampler (as), reuter centrifugal sampler (rcs), and sedimentation plates (sp). there was a good correlation of results obtained by parallel measurings of as and rcs. the rcs was more effective by the factor 11.5 than the as sampling airborne microorganisms. however, the as gaves the distribution ...19846377753
bacteria isolated from lymph nodes of california slaughter swine.lymph nodes from 280 slaughter swine from 9 california ranches were examined for the presence of aerobic microflora. genera of interest which were isolated (and percentage of animals from which they were isolated) included salmonella (4.3%), beta-hemolytic streptococci (8.2%), mycobacterium avium-complex (6.4%), corynebacterium pyogenes (0.4%), and aeromonas hydrophila (5.7%). patterns of bacterial isolations from swine herds may be of assistance in predicting herd health problems.19846383150
isolation of aeromonas hydrophila from a metropolitan water supply: seasonal correlation with clinical isolates.the occurrence of aeromonas spp. in the metropolitan water supply of perth, western australia, australia, was monitored at several sampling points during a period of 1 year. water within the distribution system conformed to international standards for drinking water but contained aeromonas spp. in numbers comparable to those in raw surface water, although this water was free of escherichia coli. coliforms and e. coli were found in raw surface waters, and aeromonas spp. were found in raw water fr ...19846385848
effects of short-term cold storage on recovery of proteases from extracellular products of aeromonas hydrophila.three protease-containing fractions were recovered by gel filtration from concentrated crude extracellular products produced by aeromonas hydrophila grown in a defined medium. the recovery of a heat-stable protease was differentially prevented when the crude preparation was stored for 48 h at -20 degrees c but was unaffected by storage of the crude preparation at either 4 or -70 degrees c. once fractionated, the heat-stable protease appeared to be unaffected by subsequent storage at 4, -20, or - ...19846385854
[sensitivity of diarrhea organisms to nifuroxazide]. 19846386203
evaluation of a two-hour method for screening pathogens from stool specimens.the 2-h stool-screening test (sst) (api system s.a., montalieu-vercieu, france) was used to screen 231 organisms yielding suspicious colonies on stool differential agars for potential pathogens. all 54 salmonellae yielded correct screens. of 14 shigellas, 9 keyed as possible shigella spp.-yersinia enterocolitica-rare salmonella spp. (sys), and 5 (all shigella sonnei) keyed as possible s. sonnei-y. enterocolitica-arizona spp. (sya). three arizona strains were identified as probable salmonella spp ...19846386849
aeromonas. 19846389407
aspirin in acute gastroenteritis: a clinical and microbiological study.soluble aspirin given by mouth in divided dosage decreased intestinal fluid loss in infants and young children with acute gastroenteritis. the treated group had significantly less diarrhea, which ceased earlier and needed less intravenous therapy, than a randomly selected control group given an indistinguishable placebo. this effect of aspirin occurred with diarrhea caused by salmonella, aeromonas, escherichia coli producing heat-labile toxin, and rotavirus, but not with diarrhea associated with ...19846389815
aeromonas hydrophila and plesiomonas shigelloides as causes of intestinal infections.infections due to aeromonas hydrophila and plesiomonas shigelloides in immuno-compromised hosts have been well documented, but the role of these organisms in causing gastrointestinal disease in healthy persons is controversial. despite difficulties in characterizing the exotoxins produced by aeromonas species, there is accumulating evidence that these bacteria are capable of causing usually mild, self-limited diarrheal disease in previously healthy adults. some pediatric patients may have protra ...19846390638
prophylactic doxycycline for travelers' diarrhea in thailand. further supportive evidence of aeromonas hydrophila as an enteric pathogen.a randomized double-blind study to determine the efficacy of a three-week course of doxycycline (100 mg daily) in preventing travelers' diarrhea was performed in 1980 among 63 united states peace corps volunteers during their first five weeks in thailand, an area where doxycycline-resistant enterotoxigenic escherichia coli are known to be common. eight (24%) of 33 volunteers taking placebo and 3 (10%) of 30 taking doxycycline developed travelers' diarrhea for a calculated protection of 59%, but ...19846391157
[demonstration of a proteolytic activity on intracellular origin in aeromonas hydrophila lp 50].aeromonas hydrophila lp 50, isolated from packaged pasteurized milk, was grown in glucose-polypeptone medium at 30 degrees c. the proteolytic activity of a. hydrophila lp 50, optimum at the stationary phase of growth, is attributed to extracellular or membrane protease; no intracellular proteolytic activity was shown.19846391644
collaborative evaluation of the abbott avantage system for identification of frequently isolated nonfermentative or oxidase-positive gram-negative bacilli.the capability of the abbott avantage system to identify 10 species of commonly isolated glucose nonfermentative or oxidase-positive gram-negative bacilli in a 5-h test period was evaluated in a collaborative study. the avantage nonenteric data base uses 20 biochemical test reactions performed in an expanded abbott bacterial identification cartridge plus the results of a manual oxidase test. the species included in the avantage data base are acinetobacter anitratus, acinetobacter iwoffi, aeromon ...19846392323
[evaluation of results in the identification of various aerobic gram-negative bacteria in selected bacteriological laboratories in poland]. 19846392790
the impact of terrestrial and estuarial factors on the density of environmental bacterial (vibrionaceae) and faecal coliforms in coastal water.a study of the densities of faecal coliforms, vibrio anguillarum, vibrio alginolyticus and aeromonas hydrophila in danish bathing areas was carried out over the period may-october, 1981 with special reference to specific terrestrial and estuarial determinants. significant correlations were found between v. anguillarum and v. alginolyticus (r = 0.57) and a. hydrophila and faecal coliforms (r = 0.43). the densities of the vibrios were highly influenced by temperature and distance from pollution so ...19846395571
[spoilage microorganisms encountered in ultra-high temperature processed milk].40 strains of aerobic or facultative anaerobic microorganisms were isolated from a total of 37 spoiled ultra high temperature processed milk. 13 of them were identified as the genus bacillus. they were 6 b. cereus, 5 b. licheniformis, 1 b. brevis and 1 b. pumilus. the other 27 strains were nonsporeforming microorganisms, which included 5 yeasts, 2 pseudomonas sp., 3 streptococcus sp., 12 lactobacillus sp., 1 shigella sp., 1 aeromonas sp. and 3 micrococcus sp. results indicate that the spoilage o ...19846468029
[distribution of enterobacteria in soil following irrigation with waste water].the filtration of wastewater after irrigation by sandy soil in the area of the braunschweig sewage utilization association was investigated. for that purpose, the concentrations of gram-negative aerobe rods were determined in soil samples of 15 cm, 30 cm, 45 cm, 60 cm, 75 cm, 90 cm, 105 cm, 120 cm, 135 cm, and 150 cm depth immediately after an irrigation of about 50 mm wastewater during the vegetation (3 series), the vegetationless period (6 series), after one week (3 series) and after two weeks ...19846475379
[aeromonas hydrophila septicemia: 3 cases, 1 with mediastinitis].three cases of aeromonas hydrophila septicaemia are reported. the first case concerned a 51 years old patient suffering from drowning who had previously been in good health and who died after a few days of refractory hypoxaemia. the second case was an 8 years old child with trisomy 21 operated for a congenital cardiac defect. the portal of entry was a catheter and the septicaemia was complicated by a. hydrophila mediastinitis, a complication which has not been described previously. the third cas ...19846476665
difference in the extracellular products of two strains of aeromonas hydrophila virulent and weakly virulent for fish.in this study we first compared the toxigenic profile of aeromonas hydrophila strains virulent and weakly virulent for rainbow trout. the separation of the toxic factor for fish is also described. both strains produced hemolytic, enterotoxic, and dermonecrotic activities; the weakly virulent strain produced 20 times more hemolysin. only the cell-free supernatant of the virulent strain produced a toxic factor for fish. after passage on sephacryl s-200, the toxic factor for fish was separated from ...19846478313
nucleotide base sequence of vibrionaceae 5 s rrna.nucleotide base sequences of 5 s rrnas isolated from vibrio vulnificus, vibrio anguillarum, and aeromonas hydrophila were determined. comparisons among these and sequences of 5 s rrnas from other species of vibrionaceae provide information useful in the evaluation of the evolution of bacterial species.19846479333
purification and some properties of aeromonas hydrophila hemolysin.a hemolysin produced by a strain of aeromonas hydrophila isolated from a patient with diarrhea was purified by acid precipitation and quarternary aminoethyl-sephadex chromatography. the molecular weight of the hemolysin was estimated at 50,000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and at 48,000 by sephadex g-100 gel filtration. in polyacrylamide gel electrophoresis at ph 4.0 and sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the hemolysin migrated as a single b ...19846480102
thromboxane interaction with cardiopulmonary dysfunction in graded bacterial sepsis.the relationship between plasma levels of thromboxane a2, radioimmunoassayed as thromboxane b2 (txb), and cardiopulmonary dysfunction in graded bacterial sepsis was investigated. five adult female pigs under anesthesia were intubated and allowed to breathe room air spontaneously. femoral arterial, venous, and pulmonary artery catheters were inserted. after a 60-minute control period aeromonas hydrophila (1.0 x 10(9)/ml) was infused intravenously at 0.2 ml/kg/hr, gradually increasing to 4.0 ml/kg ...19846481830
isolation of aeromonas spp. from an unchlorinated domestic water supply.the recovery of aeromonas spp. from the unchlorinated water supply for a western australian city of 21,000 people was monitored at several sampling points during a period of 1 year. membrane filtration techniques were used to count colonies of aeromonas spp., coliforms, and escherichia coli in water sampled before entry to service reservoirs, during storage in service reservoirs, and in distribution systems. aeromonas spp. were identified by subculture on blood agar with ampicillin, oxidase test ...19846486783
the occurrence of aeromonas hydrophila in avian diagnostic submissions.twenty isolations of aeromonas hydrophila were made from 15 species of free-living, commercial, and companion birds submitted for routine diagnostic postmortem examination. seventy percent of the isolations were made from november through march during the 25-month survey period.19846487200
structure of the lipopolysaccharide core isolated from a human strain of aeromonas hydrophila.lipopolysaccharide was isolated from the cell-walls of a human strain of aeromonas hydrophila by the aqueous phenol method in 0.58% yield (based on dry weight of bacteria). the lipopolysaccharide consisted of sr-polysaccharide, core-oligosaccharide and lipid a; there was no o-specific polysaccharide. the core had the composition d-galactose, d-glucose, d-glycero-d-manno-heptose, l-glycero-d-manno-heptose and d-glucosamine in a molar ratio of 1:1:2:4:1. glucosamine was linked to an l-glycero-d-ma ...19846489347
action of a microbial glycerophospholipid:cholesterol acyltransferase on plasma from normal and lcat-deficient subjects.the action of a bacterial acyltransferase similar in overall reaction mechanism to the plasma enzyme lecithin:cholesterol acyltransferase (lcat) has been studied using normal plasma and lipoproteins and plasma from lcat-deficient patients. the microbial enzyme (gcat) catalyzed acyl transfer using phosphatidylcholine and cholesterol in all of the lipoprotein fractions, presumably because it has no apolipoprotein cofactor. in addition, the enzyme was capable of hydrolyzing cholesteryl ester in lip ...19846491538
furunculosis in rainbow trout (salmo gairdneri) raised in sea water.ulcerative skin lesions were encountered in rainbow trout raised in sea water by a commercial concern in the western cape, south africa. grossly, the lesions resembled furunculosis but, histopathologically, they differed from typical furunculosis in that bacterial colonies were rarely found in the organs, and also the kidneys and spleens were minimally involved. the causative organism was identified as an achromogenic aeromonas salmonicida that shared characteristics with all 3 subspecies, salmo ...19846493730
aeromonas toxins. 19846494019
aeromonas infection in an infant with aplastic anemia. 19846494020
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