Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| squamous cell carcinoma of the endometrium with human papillomavirus type 31. | 1997 | 9154764 | |
| recurrent respiratory papillomatosis with pulmonary cystic disease in a child, following maternal genital warts. | human papillomavirus (hpv) infection of the genital tract is associated with the development of genital warts. a causal link between maternal hpv infection and development of laryngeal papillomatosis in the offspring has been proposed. we report a case of pulmonary cystic disease, a rare but serious complication of laryngeal papillomatosis in a child, following maternal genital warts. | 1997 | 9155559 |
| human papillomavirus infection and invasive cervical neoplasia: a study of prevalence and morphology. | the prevalence of human papillomavirus (hpv) dna sequences in 45 cervical cancer biopsies was examined with the hot-start polymerase chain reaction (pcr), employing hpv consensus primers from the l1 region. the cases comprised 38 squamous cell carcinomas, three adenosquamous carcinomas, and four adenocarcinomas. pcr products were typed with single-strand conformation polymorphism (sscp) and the hpv types detected were correlated with tumour type. forty-three biopsies were hpv-positive, hpv16 bei ... | 1997 | 9155711 |
| the incidence and detection of hpv in the upper aerodigestive tract using brush and biopsy techniques. | the association between human papillomavirus (hpv) and squamous carcinoma is well established. in the polymerase chain reaction (pcr) we have an effective technique for detecting small quantities of viral dna, but the assay requires tissue taken at endoscopy to reveal the presence or absence of hpv. brushings have been used effectively elsewhere in the body to obtain cytological material. this study set out to ascertain if sufficient viral dna could be obtained, using a laryngeal brushing and th ... | 1997 | 9156055 |
| cutaneous squamous cell carcinoma in human immunodeficiency virus-infected patients. a study of epidemiologic risk factors, human papillomavirus, and p53 expression. | to examine risk factors for the development of cutaneous squamous cell carcinoma (scc) in a group of human immunodeficiency virus (hiv)-infected patients, including evaluation and detection of epidemiologic risk factors of human papillomavirus (hpv) and p53 expression. | 1997 | 9158410 |
| [cervical intraepithelial neoplasia (cin) and human papillomavirus (hpv) infection]. | many studies have shown a strong correlation between cin and hpv infection. molecular biology has allowed identification of types of hpv which seem to be connected, more frequently than others, to dysplastic lesions. physical state of hpv-genome seems to play an important role in the development of cervical cancer. in this study the hpv-genome has been searched in tissue specimens obtained from 34 women affected by cin ii and iii. all patients underwent laser conization. immediately before treat ... | 1997 | 9162879 |
| increased prevalence of vulvovaginal condyloma and vulvar intraepithelial neoplasia in women infected with the human immunodeficiency virus. | to compare the prevalence of human papillomavirus (hpv)-associated vulvovaginal lesions in human immunodeficiency virus (hiv)-positive and hiv-negative women. | 1997 | 9166302 |
| [primary carcinoma of bartholin's glands with hpv 18 detection]. | a primary carcinoma of the bartholin's gland is a rarely type of vulva-carcinoma. the patients median age are 55 years and the etiology of the carcinoma are still unknown. viral influences are discussed. most frequent types of carcinomas of bartholin's gland are those of squamous cells and adenocarcinomas. this article reports on a 55 year old patient with a primary carcinoma of bartholin's gland. the human papillomavirus 18 was detected by a polymerase chain reaction. the correct diagnosis was ... | 1997 | 9173772 |
| frequency of hpv infection and gsh levels in plasma of women with cervical dysplasia. | human papillomavirus (hpv) can be a cause of cervical intraepithelial neoplasia (cin) and cervical cancer. the level of antioxidant compounds in plasma (retinol, alfa-tocoferol, vitamins c and e, glutathione) can modulate the progression of latent hpv infection to subclinical lesions and cin. our studies show that in the cases of hpv infection glutathione (gsh) content decreased, but glutathione disulfide (gssg) levels increased. the quantitative gsh/gssg ratio reflects the above changes of both ... | 1997 | 9174835 |
| the effect of human papillomavirus infection on sperm cell motility. | to investigate the presence of human papillomavirus (hpv) in human sperm cells and to evaluate potential effects of hpv on the sperm functions. | 1997 | 9176459 |
| hpv-16 oncogenes e6 and e7 are mutagenic in normal human oral keratinocytes. | the mutation frequency of ps189 shuttle vector plasmids is higher in human oral keratinocytes (nhok) immortalized with cloned human papillomavirus-16 (hpv-16) genome than in primary normal nhok (nhok). to determine whether oncoproteins e6 and e7 of hpv-16 are responsible for the higher mutation frequency of the plasmids, we measured the mutation frequency in nhok and in nhok expressing the hpv-16 oncogenes (e6, e7, or e6 plus e7). we also measured the mutation frequency in nhok expressing the e6 ... | 1997 | 9178911 |
| physical and functional interaction between the human t-cell lymphotropic virus type 1 tax1 protein and the ccaat binding protein nf-y. | tax1, a potent activator of human t-cell lymphotropic virus type 1 (htlv-1) transcription, has been shown to modulate expression of many cellular genes. tax1 does not bind dna directly but regulates transcription through protein-protein interactions with sequence-specific transcription factors. using the yeast two-hybrid system to screen for proteins which interact with tax1, we isolated the b subunit of the ccaat binding protein nf-y from a hela cdna library. the interaction of tax1 with nf-yb ... | 1997 | 9032250 |
| ccaat displacement protein, a regulator of differentiation-specific gene expression, binds a negative regulatory element within the 5' end of the human papillomavirus type 6 long control region. | we have reported previously that a 636-bp fragment spanning the 5' two-thirds of the human papillomavirus type 6 (hpv6)-w50 long control region (lcr) functions as a transcriptional silencer (a. farr, s. pattison, b.-s. youn, and a. roman, j. gen. virol. 76:827-835, 1995). we have utilized nested deletion analyses to implicate a 66-bp sequence which appears to be critical for this activity. a comparison of the transcriptional regulatory activities of the lcrs of hpv6-w50 and hpv6b (which has a 94 ... | 1997 | 9032333 |
| human papillomavirus types 52 and 58 are prevalent in cervical cancers from chinese women. | a substantial body of evidence has confirmed human papillomavirus (hpv) infection as an etiologic agent in human cervical cancer. to evaluate the association between hpv and cervical cancer in chinese women, we examined tumor specimens from women who lived in shanghai, people's republic of china. biopsies from 40 women, diagnosed with either squamous-cell carcinoma (n = 35) or adenocarcinoma (n = 5) were tested for hpv dna by pcr. the hpv types present in tumors were determined either by hybridi ... | 1997 | 9033647 |
| absence of human papillomavirus infection in cutaneous lichen planus. | 1997 | 9039186 | |
| a general primer gp5+/gp6(+)-mediated pcr-enzyme immunoassay method for rapid detection of 14 high-risk and 6 low-risk human papillomavirus genotypes in cervical scrapings. | two cocktails of digoxigenin-labeled human papillomavirus (hpv) type-specific oligonucleotide probes and an enzyme immunoassay (eia) were used as a basis to developed a group-specific detection method for 14 high-risk (types 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, and 68) and 6 low-risk (types 6, 11, 40, 42, 43, and 44) hpvs, following a general primer gp5+/biogp6(+)-mediated pcr. the sensitivity of this high-risk/low-risk (hr/lr) hpv pcr-eia ranged from 10 to 200 hpv copies, dependi ... | 1997 | 9041439 |
| juvenile laryngeal papillomatosis in a pediatric population: a clinicopathologic study. | a series of 22 children with juvenile laryngeal papillomatosis treated over a 31-year period is presented. the majority of patients were diagnosed when less than 5 years of age. two patients died from the disease and four patients still had active disease at the completion of the study period. the duration of disease and number of recurrences were extremely variable. the number of recurrences was inversely related to the age of onset. the histologic findings were very similar in all patients, an ... | 1997 | 9050060 |
| synchronous and metachronous multicentric squamous cell carcinomas in the upper aerodigestive tract. | a rare case is presented of a 57-year-old japanese male with synchronous and metachronous multicentric squamous cell carcinomas (scc) in the upper aerodigestive tract. during a 9-year-period from the appearance of first primary scc to autopsy, 14 foci of primary scc and one severe dysplasia developed in succession in the thoracic esophagus, oral floor, soft palate, uvula, lingual radix, piriform recess, hypopharynx, cervical esophagus, trachea and lingual body. the patient died of severe broncho ... | 1997 | 9051695 |
| human papillomavirus dna and antibodies to human papillomaviruses 16 e2, l2, and e7 peptides as predictors of survival in patients with squamous cell cervical cancer. | to assess whether human papillomavirus (hpv) dna detection in cervical cancer specimens, or antibodies to selected hpv 16 peptides are predictors of tumor recurrence and long-term survival in patients with squamous cell invasive cervical cancer. | 1997 | 9053484 |
| 20q gain associates with immortalization: 20q13.2 amplification correlates with genome instability in human papillomavirus 16 e7 transformed human uroepithelial cells. | breast, bladder, colon, and ovarian carcinomas show frequent low level 20q gain and less frequently high level 20q13.2 amplification, but the significance of these 20q amplifications in transformation has not been defined. using karyotypic and comparative genomic hybridization (cgh) analyses, chromosome losses and gains were analysed in six newly immortalized human uroepithelial cell (huc) lines transformed by human papillomavirus 16 (hpv16) e7. results showed clonal chromosomes with 20q11->qter ... | 1997 | 9053853 |
| human papillomavirus e6 and e7 oncoproteins alter cell cycle progression but not radiosensitivity of carcinoma cells treated with low-dose-rate radiation. | low-dose-rate radiation therapy has been widely used in the treatment of urogenital malignancies. when continuously exposed to low-dose-rate ionizing radiation, target cancer cells typically exhibit abnormalities in replicative cell-cycle progression. cancer cells that arrest in the g2 phase of the cell cycle when irradiated may become exquisitely sensitive to killing by further low-dose-rate radiation treatment. oncogenic human papillomaviruses (hpvs), which play a major role in the pathogenesi ... | 1997 | 9054890 |
| detection of human papillomavirus dna in keratoacanthomas by polymerase chain reaction. | the etiology of keratoacanthomas is unknown, but human papillomavirus (hpv) has been suspected to be involved in the pathogenesis of this lesion because koilocytic changes may be observed and because hpv has been found in cutaneous squamous-cell carcinomas and premalignant keratoses in immunosuppressed patients. we analyzed dna extracted from 39 keratoacanthomas from 22 "at-risk" patients (nine patients undergoing uv light and/or anthralin therapy for psoriasis, 10 solid organ transplant recipie ... | 1997 | 9056648 |
| renal allograft recipients with high susceptibility to cutaneous malignancy have an increased prevalence of human papillomavirus dna in skin tumours and a greater risk of anogenital malignancy. | renal allograft recipients (rars) have a well-documented increased incidence of viral warts and cutaneous neoplasia, particularly those with long graft life and high sun exposure. a clinicopathological survey of 69 rars in south-east scotland, with follow-up periods of up to 28 years after transplantation, revealed marked variation in patient susceptibility to cutaneous malignancy with concomitant variation in hpv prevalence. skin cancers were found in 34 patients. eight patients showed high sus ... | 1997 | 9043031 |
| transfection of the inner cell mass and lack of a unique dna sequence affecting the uptake of exogenous dna by sperm as shown by dideoxy sequencing analogues. | the purpose of this study was to determine whether exogenous dna internalized into blastocysts after transference from dna-carrier sperm are localized at the inner cell mass or trophoblast cells and to identify differences in uptake of exogenous dna fragments by sperm due to unique dna sequences. | 1997 | 9048243 |
| prevalence of human papillomavirus dna in cervical tissue. retrospective analysis of 855 cervical biopsies. | the histopathologic features of 855 cervical biopsies were correlated with the presence of human papillomavirus dna using in situ hybridization (ish) with biotin labeled type specific probes for human papilloma virus (hpv) types 6, 11, 16, 18, 31, 33 and 51. hpv-dna was found in 18% (13/72) of cervical intraepithelial neoplasia i (cin i), 30% (35/115) of cin ii, 28% (57(206) of cin iii, in 84% (21/25) of flat condyloma and in 13% (15/112) of normal cervical tissue. hpv dna was detectable in 11% ... | 1997 | 9059747 |
| analysis of the p53-mediated g1 growth arrest pathway in cells expressing the human papillomavirus type 16 e7 oncoprotein. | cells expressing human papillomavirus type 16 (hpv-16) e7, similar to those which express hpv-16 e6, are resistant to a p53-mediated g1 growth arrest. we examined the p53-mediated dna damage response pathway in e7-expressing cells to determine the mechanism by which e7-containing cells continue to cycle. in response to dna damage, no dramatic difference was detected in g1- or s-phase cyclin or cyclin-dependent kinase (cdk) levels when e7-expressing cells were compared to the parental cell line, ... | 1997 | 9060648 |
| genital human papillomavirus infections: knowledge, perception of risk, and actual risk in a nonclinic population of young women. | the purpose of this study was to assess knowledge, beliefs, and attitudes about genital human papillomavirus (hpv) infections in a group of young women in a nonclinic setting and to examine the association between perception of risk and actual risk. one hundred ten women attending a state university participated in the study and were asked to complete a self-administered questionnaire about knowledge and perceptions of risk. they were then offered testing for the virus using a self-administered ... | 1997 | 9065380 |
| investigation of oesophageal adenocarcinoma for viral genomic sequences. | overexpression of the tumour suppressor gene product p53 is common in oesophageal adenocarcinoma. this may be due to gene mutation, but overexpression can also result from complexing between viral proteins and p53; a number of viruses are causally linked with malignancy. this study therefore investigated the prevalence in oesophageal adenocarcinoma of viruses whose gene products are capable of interacting with p53. seventeen tumours and 17 normal oesophagi were screened for specific dna sequence ... | 1997 | 9066743 |
| verrucous carcinoma or epithelioma cuniculatum plantare. | verrucous carcinoma is a rare form of squamous cell carcinoma. it behaves as a slow-growing, locally aggressive and rarely metastasizing tumour. three clinical cases are presented, with emphasis on clinical presentation, pathological diagnosis and therapeutic approach. the pathogenesis of verrucous carcinoma, including chronic inflammation and human papillomavirus, is also discussed. | 1997 | 9066754 |
| demographic data on prostitutes from bulgaria--a recruitment country for international (migratory) prostitutes. | the study was aimed at investigating the conditions and circumstances for the recruitment of prostitutes, as well as their reproductive history, working conditions, knowledge of and attitudes to sexually transmitted diseases (stds), and use of prophylactic antibiotic therapy of these diseases. two hundred prostitutes were investigated by in-depth interviews at std clinics, private practices and hotels. of the 200 prostitutes, 8 (4%) were less than 15 years old and 32 (16%) more than 25 years old ... | 1997 | 9089030 |
| cervical pathology. | this review concentrates on recent developments in several distinct areas of cervical pathology. the potential clinical use of human papillomavirus typing and determination of proliferation using immunohistochemical techniques is discussed together with the current status of prognostic markers in cervical disease. recent literature on glandular neoplasia and changes that mimic it is reviewed, and descriptions of new and rare entities, including lesions showing mesonephric differentiation, are di ... | 1997 | 9090484 |
| glandular lesions of the uterine cervix: prognostic implications of human papillomavirus status. | endocervical adenocarcinoma in situ (ais) is believed to be a precursor of invasive disease; however, the biologic behavior of endocervical glandular "atypias" (gas) is unclear. the purpose of this study was to evaluate the potential role of ga as a precursor of adenocarcinoma by assessment of the human papillomavirus (hpv) status of glandular lesions. we analyzed by polymerase chain reaction (pcr) 69 cases within a spectrum of endocervical glandular lesions encompassing invasive adenocarcinoma ... | 1997 | 9100062 |
| cytologic atypia. clinical significance and follow-up recommendations. | to assess the evolution of cytologic atypia on cervical cytologic smears as an attempt to better understand the natural history of these lesions and to establish a follow-up protocol. | 1997 | 9100788 |
| design and methods of a population-based natural history study of cervical neoplasia in a rural province of costa rica: the guanacaste project. | this paper reports on the enrollment phase of a population-based natural history study of cervical neoplasia in guanacaste, a rural province of costa rica with consistently high rates of invasive cervical cancer. the main goals of the study are to investigate the role of human papillomavirus (hpv) infection and its co-factors in the etiology of high-grade cervical neoplasia, and to evaluate new cervical cancer screening technologies. to begin, a random sample of censal segments was selected and ... | 1997 | 9180057 |
| p53 mutations in relation to human papillomavirus type 16 infection in squamous cell carcinomas of the head and neck. | the relationship between human papillomavirus (hpv) type 16 infection and p53 gene mutations was investigated in squamous cell carcinomas of the head and neck (scchn). hpv was detected by general primer-mediated and type-specific pcr. alterations in the p53 gene were investigated using single-strand conformation polymorphism and sequence analysis in 27 scchn, of which 12 were hpv 16-positive and 15 were hpv-negative. mutations were detected in 2/12 (16.7%) hpv 16-positive and 7/15 (46.7%) hpv-ne ... | 1997 | 9180148 |
| human papillomavirus variants: implications for natural history studies and vaccine development efforts. | 1997 | 9182967 | |
| probing for cerebrospinal fluid antibody specificities by a panel of random peptide libraries. | in a state of health the central nervous system is nearly devoid of macrophages, t and b cells. however, such cells are required to initiate the inflammation associated with multiple sclerosis (ms). an important issue in understanding the pathogenesis of such conditions is to fully define the t and b cell specificities. using random peptide libraries, we have analysed the cerebrospinal fluid antibody specificities within one oligoclonal "band" from a patient with ms. the selected peptides from a ... | 1997 | 9189009 |
| presence of human papillomavirus in esophageal squamous cell carcinomas of hong kong chinese and its relationship with p53 gene mutation. | there is no scientific study that has investigated the association between human papilloma virus (hpv) and p53 mutation in hong kong chinese patients with esophageal cancers. the aim of this survey is to evaluate in details the prevalence and relationship of hpv and p53 mutation in these patients with esophageal squamous cell carcinomas. fresh tissues from the resected specimens of 70 chinese patients (59 men, 11 women) with primary esophageal squamous cell carcinomas (20 well-differentiated, 36 ... | 1997 | 9190999 |
| interlaboratory agreement among results of human papillomavirus type 16 enzyme-linked immunosorbent assays. | serological assays for measuring antibodies to human papillomavirus type 16 (hpv-16) virus-like particles (vlps) have become important epidemiologic tools in recent years. however, the interlaboratory replicability of these assays has not been assessed. in this investigation, three laboratories tested a panel of specimens obtained from two different groups: 265 subjects in a vulvar cancer case-control study and 107 healthy volunteer blood donors. each laboratory used an enzyme-linked immunosorbe ... | 1997 | 9196186 |
| human papilloma virus 16-18 infection and cervical cancer in mexico: a case-control study. | cervical cancer (cc) is one of the principal public health problems in mexico. the national mortality rate due to cc was estimated at 21.8 per 100,000 among women over 15 years old during 1994. despite this high incidence little is known in mexico about the risk factors for cc. the objectives of the study were to evaluate the association between cc and hpv types 16 and 18 in women living in mexico city. from august, 1990 to december, 1992, a case-control study was carried out in the metropolitan ... | 1997 | 9204620 |
| two cytodifferentiation agent-induced pathways, differentiation and apoptosis, are distinguished by the expression of human papillomavirus 16 e7 in human bladder carcinoma cells. | many transformed cells have been found to lose the capacity to proliferate and undergo differentiation following exposure to hybrid polar agents. this study investigates the mechanism by which hexamethylene bisacetamide (hmba) suppresses the proliferation of the human bladder carcinoma line, t24. we found that following a 24-h exposure to hmba, t24 proliferation was inhibited, and cells arrested in g1 phase and underwent morphological maturation. hmba-induced cessation of proliferation was media ... | 1997 | 9205091 |
| lymphoepithelioma-like carcinoma of the uterine cervix: association with epstein-barr virus and human papillomavirus. | the presence of epstein-barr virus (ebv) has not been documented in previous reports of lymphoepithelioma-like carcinoma (lelc) of the uterine cervix by either polymerase chain reaction or in situ hybridization, and the histogenesis of the tumor remains unknown. additionally, a relationship between human papillomavirus (hpv) and cervical lelc also has not been reported. | 1997 | 9210713 |
| ha-ras oncogene-induced transcription of human papillomavirus type 18 e6 and e7 oncogenes. | human papillomavirus (hpv) dna sequences are found in most carcinomas originating from the uterine cervix. hpv e6 and e7 oncogenes have been shown to cooperate with ras oncogenes to fully transform human epithelial cells. we investigated the effect of the ha-ras oncogene on the transcriptional activity of hpv-18 and found that it induced the transcriptional activity of the viral promoter, whereas the normal gene had only a minimal effect. however, transfection of the normal ha-ras gene and simul ... | 1997 | 9210955 |
| complex genomic rearrangement within the 12q15 multiple aberration region induced by integrated human papillomavirus 18 in a cervical carcinoma cell line. | human papillomavirus (hpv) dna is integrated into the host genome in cervical cancer. the cervical carcinoma cell line sw756 has integrated hpv-18 dna in chromosome region 12q15, in the papillomavirus-associated locus-2 (pal2). by polymerase chain reaction and hybridization of an arrayed cosmid library with oligonucleotides from the rearranged allele, we determined the pre-integration germline structure of the region. pal2 was located approximately 10 kb from sequence-tagged site marker u27131, ... | 1997 | 9210958 |
| androgen responsive adult human prostatic epithelial cell lines immortalized by human papillomavirus 18. | prostate cancer and benign tumors of the prostate are the two most common neoplastic diseases in men in the united states, however, research on their causes and treatment has been slow because of the difficulty in obtaining fresh samples of human tissue and a lack of well characterized cell lines which exhibit growth and differentiation characteristics of normal prostatic epithelium. non-neoplastic adult human prostatic epithelial cells from a white male donor were immortalized with human papill ... | 1997 | 9214605 |
| acinar differentiation by non-malignant immortalized human prostatic epithelial cells and its loss by malignant cells. | invasive prostatic carcinomas and prostatic intraepithelial neoplasia (pin) are characterized by a loss of normal cell organization, cell polarity, and cell:cell and cell:basement membrane adhesion. the objective of this study was to establish in vitro three-dimensional (3-d) cell models which can be used to investigate mechanisms involved in acinar morphogenesis and differentiation in normal prostatic epithelium and their abnormalities in cancer cells. the process of acinar morphogenesis, inclu ... | 1997 | 9214606 |
| detection of human papillomavirus infection in squamous tumours of the conjunctiva and lacrimal sac by immunohistochemistry, in situ hybridisation, and polymerase chain reaction. | squamous tumours of the ocular surface, including the lacrimal pathway, range from benign lesions to invasive carcinomas. some of these tumours are associated with human papillomavirus (hpv) infection, with the types of hpv differing among papillomas and dysplastic or malignant lesions. | 1997 | 9215061 |
| seropositivity against hpv 16 capsids: a better marker of past sexual behaviour than presence of hpv dna. | to assess if seropositivity to human papillomavirus type 16 capsids is a better marker of sexual history than the presence of hpv dna. | 1997 | 9215097 |
| human papillomaviruses are not associated with renal carcinoma. | in an attempt to better understand the troubling issue of renal human papillomavirus (hpv) infection, fresh tumour specimens obtained by surgical extirpation from tumours were compared for the presence of hpv. all of the renal carcinoma samples were examined by pcr using two sets of consensus primers and the specific primer pairs for hpv 16, 18, and 33. none of the 28 carcinomas and 17 corresponding normal tissues were found positive for hpv dna in any of the applied analyses. our results sugges ... | 1997 | 9216686 |
| p53 protein overexpression in laryngeal squamous cell papillomas. | we retrospectively investigated p53 protein immunoreactivity in 103 laryngeal squamous cell papillomas (lp) previously revealed to be human papillomavirus type 6 or 11 positive by in situ hybridization and/or the polymerase chain reaction. 21 lp failed to show any detectable level of p53 protein reactivity. in 45 cases only occasional strongly positive cells were observed in almost the whole thickness of the epithelium. in 26 lp, p53 protein immunoreactivity was found to be almost exclusively re ... | 1997 | 9216688 |
| coinfection of hpv-11 and hpv-16 in a case of laryngeal squamous papillomas with severe dysplasia. | human papillomavirus (hpv) types 6 and 11 have been associated with benign laryngeal papilloma, while hpv-16 is occasionally associated with laryngeal carcinoma. in this study, a case of laryngeal squamous papillomas with severe dysplasia was evaluated for the presence of hpv infection. the biopsy specimens were taken from a 58-year-old female patient at two different time points 3 months apart. architecturally, the tumor showed papillary configuration reminiscent of squamous papilloma. cytologi ... | 1997 | 9217136 |
| perspectives on human papillomavirus infection. | 1997 | 9217655 | |
| human papillomavirus and human disease. | human papillomaviruses (hpvs) are associated with a spectrum of different diseases in humans, including common warts and genital warts. of more serious concern is the connection between certain hpv types and some malignancies, particularly cervical and anal cancer. dna from hpv-16 and hpv-18, two types frequently found in cervical cancer tissue, can immortalize cells in laboratory cultures, unlike dna from hpv types associated with benign genital lesions. although it is unclear how high-risk hpv ... | 1997 | 9217657 |
| clinical presentation and natural course of anogenital warts. | the natural history of human papillomavirus (hpv) genital tract lesions is complex, partly because infection can appear in several forms and often follows a fluctuating course. the primary mode of transmission of genital strains of hpv is through sexual contact. most clinically apparent genital warts are caused by hpv type 6 or 11. the main manifestations of anogenital warts are cauliflower-like condylomata acuminata that usually involve moist surfaces; keratotic and smooth papular warts, usuall ... | 1997 | 9217658 |
| counseling patients with genital warts. | counseling patients about any sexually transmitted disease (std) is difficult, for both the physician and the patient, but a diagnosis of genital warts presents particular challenges. for many patients, being told that they have any std comes as a shock. although fear is a common reaction, the relationship between human papillomavirus (hpv) and cancer has made the presence of genital warts especially frightening. this fear is heightened by the fact that treatment will not eradicate the underlyin ... | 1997 | 9217661 |
| mutational analyses of differentiation-dependent human papillomavirus type 18 enhancer elements in epithelial raft cultures of neonatal foreskin keratinocytes. | human papillomaviruses (hpvs) reproduce only in differentiated squamous epithelia. viral transcription is rather restricted in basal strata but increases dramatically in the spinous cells. inopportune viral oncoprotein expression in the basal reserve cells can lead to dysplasias and carcinomas. until now all studies to identify transcription factor binding sites within the upstream regulatory region (urr) that controls the expression of the oncogene have been conducted in proliferating cell cult ... | 1997 | 9218869 |
| two classes of human papillomavirus type 16 e1 mutants suggest pleiotropic conformational constraints affecting e1 multimerization, e2 interaction, and interaction with cellular proteins. | random mutagenesis of human papillomavirus type 16 (hpv16) e1 was used to generate e1 missense mutants defective for interaction with either hubc9 or 16e1-bp, two cdnas encoding proteins that have been identified by their ability to interact with hpv16 e1 in two-hybrid assays. hubc9, the human counterpart of saccharomyces cerevisiae ubc9, is a ubiquitin-conjugating enzyme known to be involved in cell cycle progression. 16e1-bp encodes a protein of no known function but does contain an atpase sig ... | 1997 | 9223484 |
| characterization of a human papillomavirus type 16 variant-dependent neutralizing epitope. | we have determined that three type-specific and conformationally dependent monoclonal antibodies, h16.e70, h16.u4, and h16.v5, neutralize pseudotype human papillomavirus type 16 (hpv16) virions in vitro. h16.u4 and h16.v5 neutralized pseudotype virions derived from the german hpv16 variant 114k and the zairian variant z-1194 with equal efficiency. in contrast, neutralization of z-1194 pseudotype virions by h16.e70 was two orders of magnitude weaker than neutralization of 114k pseudotype virions. ... | 1997 | 9223527 |
| prevalence of human papilloma virus 16 or 18 in cervical cancer in hualien, eastern taiwan. | in order to determine the association of human papilloma virus (hpv) with cervical cancer of patients in the hualien area, we analyzed 40 cervical cancer specimens using polymerase chain reaction (pcr) to detect the presence of hpv type 16 (hpv-16) and type 18 (hpv-18) genomes. the results showed that at least 70% (28/40) of the specimens had hpv dna. of the 37 squamous cell carcinomas of the cervix, hpv-16 was present in 25 cases (68%) and hpv-18 in 2 (5.4%). hpv-16 dna was detected in one of 2 ... | 1997 | 9226973 |
| clinical and molecular aspects of squamous cell carcinoma of the head and neck in the nonsmoker and nondrinker. | most molecular analyses of head and neck squamous cell carcinoma involve patients who have used tobacco and alcohol. the pathways involved in tumorigenesis in patients lacking these lifestyle risks may be quite different. research involving rigorous epidemiologic and molecular methods is needed to identify the unique spectra of genetic alterations in subsets of the population. this review considers recent information on the accumulation of genetic changes in the typical head and neck squamous ce ... | 1997 | 9229148 |
| infiltration of cervical cancer tissue with human papillomavirus-specific cytotoxic t-lymphocytes. | ctls specific for high-risk human papillomaviruses (hpvs) have recently been found in the peripheral blood of cervical cancer patients. although cell-mediated immunity is thought to be important in the control of hpv infection, the functional relevance and site of activation of hpv-specific ctls are unclear. we identified hla-a*0201-restricted hpv-16 e7 peptide-specific ctls in the peripheral blood (four of five patients), draining lymph nodes (three of four patients) and tumors (one of three pa ... | 1997 | 9230206 |
| alternatively spliced hpv-18 e6* protein inhibits e6 mediated degradation of p53 and suppresses transformed cell growth. | the e6 proteins originating from the tumour-associated human papillomavirus (hpv) types 16 and 18 have been shown to bind to and target the tumour suppressor protein, p53, for ubiquitin-mediated degradation. however, in cell lines derived from cervical neoplasias, the predominant early region transcripts are spliced and encode truncated forms of e6, termed e6*. we report here that hpv-18 e6* protein will interact both with the full-length e6 proteins from hpv-16 and hpv-18 and also with e6-ap, a ... | 1997 | 9233760 |
| p53-mediated accumulation of hypophosphorylated prb after the g1 restriction point fails to halt cell cycle progression. | this study analyses whether the inability of p53 to induce g1 arrest after the restriction point relates to an inability to modulate prb phosphorylation. transient p53 overexpression in normal human diploid fibroblasts and p53-deficient cancer cells led to increased levels of the cyclin-dependent kinase inhibitor p21 cip1/waf1/sdi1 and an accumulation of hypophosphorylated prb in cells growing asynchronously and in cells synchronized in late g1 or m. similarly, gamma-irradiation of asynchronous, ... | 1997 | 9233768 |
| antibodies to human papillomavirus 16 and subsequent in situ or invasive cancer of the cervix. | our objective was to examine whether past infection with human papillomavirus (hpv)-16, as determined by an antibody assay, is a risk factor for subsequent cervical cancer. incident cases of in situ or invasive cervical cancer occurring between 1975 and 1990 in a cohort of over 11,000 healthy women in washington county, md, were identified. the baseline sera of cases and of matched controls, collected in 1974, were examined for igg antibodies reactive with virus-like particles of hpv-16, a cance ... | 1997 | 9107427 |
| specific chromosomal imbalances in human papillomavirus-transfected cells during progression toward immortality. | high risk human papillomaviruses (hpvs) known to be closely associated with cervical cancer, such as hpv16 and hpv18, have the potential to immortalize human epithelial cells in culture. four lines of hpv-transfected keratinocytes were analyzed by comparative genomic hybridization at different time points after transfection. a number of chromosomal imbalances was found to be highly characteristic for the cultures progressing toward immortality. whereas several of these were new and previously no ... | 1997 | 9108068 |
| epidemiologic analysis of breast and gynecologic cancers. | this review focuses on etiologic factors and hormonal correlates of the three major gynecologic cancers-uterine cervix, uterine corpus and ovary- and breast cancer. the incidence rate of the three gynecologic cancers combined is only 40 percent of the breast cancer rate (43.6 vs 109.5 per 100,000), whereas the combined mortality rate is half that for breast cancer (14.3 vs 27.3 per 100,000). cervical cancer is distinctive in that it's hormonal correlates are few; it exhibits the epidemiologic ch ... | 1997 | 9108587 |
| [sexuality and std infections among teenage girls. "unprotected sex" is common in spite of sufficient knowledge about the risks]. | a 2-year prospective study of sexual behaviour transmitted diseases (stds) in 98 healthy 16-year-old schoolgirls showed human papillomavirus (hpv) infections to have spread rapidly among sexually active girls, whereas none of the virgins manifested hpv dna in the cervix or anti-hpv 16 or anti-hpv33 antibodies in serum. a high level of knowledge of stds and their prevention failed to induce appropriate behaviour among the sexually active. | 1997 | 9072650 |
| dna analysis indicates patient-specific human papillomavirus type 16 strains in bowen's disease on fingers and in archival samples from genital dysplasia. | human papillomavirus (hpv) type 16 is casually involved in the pathogenesis of anogenital cancer and has also been demonstrated in some patients with bowen's disease (bd) on the fingers. from two women with hpv 16 in bd on the fingers, and in archival samples from genital dysplasia, collected as long as 26 years ago, the non-coding region of the virus was amplified by the polymerase chain reaction and sequenced. the hpv 16 dna sequences found in the finger lesions and in the genital archival sam ... | 1997 | 9205498 |
| deletions of chromosome 3p are frequent and early events in the pathogenesis of uterine cervical carcinoma. | to study the molecular abnormalities involved in the multistage development of cervical carcinoma (cc), we investigated the presence of oncogenic human papillomavirus (hpv) sequences, loss of heterozygosity (loh), and microsatellite alterations at several genes/loci at 3p (3p14.2 at the fhit gene, 3p14.3-21.1, 3p21, and 3p22-24.2), 9p21, rb and p53, and p53 gene point mutations in precisely microdissected archival tissues from 20 ccs and their accompanying precursor lesions (cervical intraepithe ... | 1997 | 9242443 |
| [follow-up of high risk, human papillomavirus (hpv)-positive patients with cancer of the uterine cervix]. | the study population consisted of 30 cervical cancer patients stage i.a-ii.b. (figo) stages operated on according to the wertheim technique. a parallel histological evaluation and hpv status determination were carried out on biopsies from the primary tumors and on the regional lymph nodes. a general primer mediated polymerase chain reaction (pcr) was performed at first and the samples not amplified were examined by type-specific primers. all except one primary tumors contained dna-sequences char ... | 1997 | 9244858 |
| cytomorphologic evidence of human papillomavirus infection in smears from the irradiated uterine cervix. | to report the cytomorphologic evidence of human papillomavirus (hpv) infection in cervical smears from women treated with radiotherapy for carcinoma of the uterine cervix. | 1997 | 9250302 |
| human papillomavirus antibody responses among patients with incident cervical carcinoma. | the human papillomavirus (hpv) is recognized as a major cause of cervical cancer precursor lesions. hpv serology is a key method in the continuing elucidation of the importance of hpv exposure for cancer development and in predicting hpv-associated diseases. to extend previous hpv serological studies on cervical cancer, serum samples from a consecutive series of 216 women with incident untreated cervical carcinoma and 243 age- and sex-matched healthy blood donors were evaluated for the presence ... | 1997 | 9260694 |
| association between human papillomavirus type 18 variants and histopathology of cervical cancer. | 1997 | 9274919 | |
| two different types of human papillomavirus in a patient with carcinomas of the vulva and cervix. | 1997 | 15511786 | |
| detection of human papillomavirus in vulval carcinoma using semi-nested pcr and restriction enzyme typing: a rapid and sensitive technique. | aims-to develop a highly sensitive technique for the reliable detection and typing of human papillomavirus (hpv) dna in clinical tissue.methods-a two step, semi-nested pcr was used with primers spanning the l1 region of the hpv genome and capable of detecting hpv dna of all known hpv types. the clinical samples were typed by digestion of the 412 base pair pcr product with rsa i, generating unique fragments for each hpv type. thirteen samples were screened by this method, including nine vulval ca ... | 1996 | 16696082 |
| human papilloma virus detection by in situ hybridisation signal amplification based on biotinylated tyramine deposition. | aim-to describe a method for amplifying human papilloma virus (hpv) in situ hybridisation (ish) signals.methods-three human cervical cell lines, namely caski, hela and siha, containing different copy numbers of integrated hpv dna were studied. following ish, catalysed reporter deposition (card), based on the deposition of biotinylated tyramine at the location of the dna probe, was used to amplify the ish signal.results-using card-ish, one to three hpv type 16 copies were detected in situ both in ... | 1996 | 16696100 |
| different susceptibility of cervical keratinocytes containing human papillomavirus to cell-mediated cytotoxicity. | to detect the factors responsible for the susceptibility of cervical keratinocytes infected with human papillomavirus (hpv) to non-specific lysis mediated by natural killer (nk) and lymphokine activated killer (lak) cells. | 1996 | 9275369 |
| [clinicopathological features of human papilloma virus associated squamous cell carcinomas of the vulva]. | to investigate the relationship between human papilloma virus (hpv) status and clinicopathological features of vulva carcinoma and its clinical significance. | 1996 | 9275429 |
| [role of pcr and dot bolt hybridization in the detection of human papillomavirus of the penile cancer]. | human papillomavirus (hpv), a oncogenicpotential, may be carcinogenic effectors in variety of genital tract malignancies. we examined the association of human papillomavirus type 16 and 18 in 28 penile cancers by the highly sensitive polymerase chain reaction and dot bolt hybridization. human papillomavirus type 16 was found in 14 of 28 penile cancers (50%), whereas type 18 was identified in 3 of 28 penile cancers (10.6%). our results suggest humanpapillomavirus type 16 may play a role in the de ... | 1996 | 9275681 |
| [study on the interrelationship between human papilloma virus infection and langerhans cell in carcinogenesis of esophagus]. | in order to find out the interrelationship between human papilloma virus (hpv) infection and langerhans cells (lcs) during the development of esophageal carcinoma, and the mechanism of carcinogenesis during hpv infection, digoxigenine labelled hpv dna probes of hpv 6b/11, hpv 16/18 with in situ hybridization, and anti-s-100 protein antibody with immunohistochemical lsab-assays were used respectively in order to investigate hpv infection and the distribution of lcs in 40 cases of esophageal squam ... | 1996 | 9206210 |
| hla types in women with cervical human papillomavirus (hpv) lesions prospectively followed up for 10 years. | certain genotypes of hpv have been recently implicated in the etiology of carcinoma of the uterine cervix. in order to determine whether hla antigen-controlled immunoregulatory functions have a role in the pathogenesis of hpv infections, class i and ii hla antigen typing was carried out on a series of 96 randomly selected women who were part of a cohort of 530 women prospectively followed up for cervical hpv infections in our clinic since 1981. the frequency of the dq3 antigen, which has previou ... | 1996 | 9074659 |
| genital viral infections. studies on human papillomavirus and epstein-barr virus. | the concept 'sexually transmitted diseases' (std) was introduced in the nineteen sixties and comprises many diseases of varying importance from scabies to hiv infection. the std family has grown wider and new members will probably join. the starting point of these studies was that patients with hpv infection, attending the std clinic, appeared to increase in number in the late eighties. hpv was analysed with the southern blot technique from portio cervix in a female population attending the std ... | 1996 | 9111847 |
| pregnancy and cervical infection with human papillomaviruses. | this investigation was undertaken to assess whether pregnancy represents a risk factor for human papillomavirus (hpv) infection which is independent of age and other known hpv risk factors. | 1996 | 9236309 |
| colposcopy as a tool for detection of human papillomavirus infection of uterine cervix in the setting of high prevalence of gynaecologic infections. | a cross sectional study involving 257 women from the maternal and child health centre (mch) in delhi was initiated for screening clinically, cytologically, colposcopically, and microbiologically for several gynaecologic infections. eighty percent of the women had one or more gynaecologic infections and 31.1% had three or more infections. cytology revealed changes suggestive of condyloma in 3 (1.2%) women only, while colposcopic examination suggested hpv changes in 117 (45.5%) women. a very high ... | 1996 | 9104055 |
| detection and typing of human papillomavirus in cervical cancer in the thai. | one hundred formalin-fixed paraffin embedded tissues with histopathologic diagnosed invasive cervical cancer (squamous cell carcinoma) were examined for the presence of hpv-dna by polymerase chain reaction (pcr) using l1-consensus primers. the results indicated that 82 out of 100 (82%) samples were positive for hpv-dna. among the positive samples, 50 samples (61%) were typed by dot hybridization technique (dh). hpv-16 was the dominant type (42.68%), followed by hpv-18 (20.73%) and hpv-33 (3.66%) ... | 1996 | 9071068 |
| cancer-associated human papillomavirus types are selectively increased in the cervix of women in the first trimester of pregnancy. | because of incomplete data in the current literature, we sought to determine whether pregnancy is an independent risk for the detection of human papillomavirus infection. | 1996 | 9065116 |
| molecular diagnosis of persistent human papilloma virus infections. | persistent infections with human papilloma viruses (hpv) can lead to a wide range of benign and malignant epithelial tumors as cutaneous warts or anogenital cancers. methods principally available for the laboratory diagnosis of hpv infections are cytology and histology, immunocytochemistry, electron microscopy, serology and methods for the detection of hpv-specific dna. among the latter are polymerase chain reaction (pcr), southern blot, dot blot, filter in situ hybridization, in situ hybridizat ... | 1996 | 9058167 |
| comparison of the cervical cytology test using the papnet method and conventional microscopy. | from august 1994 to june 1995, laboratories in 28 mexican states and the federal district submitted a total of 10098 diagnosed pap test slides to mexico's national institute of epidemiologic diagnosis and reference (indre) for reexamination by conventional methods and also by the automated papnet system in suffern, new york, u.s.a. the aim was to determine the degree of agreement obtained by these various methods. most of the slides examined (at least 78%) yielded negative results or merely indi ... | 1996 | 9041745 |
| the causal link between hpv and cervical cancer and its implications for prevention of cervical cancer. | this article reviews epidemiologic evidence linking human papillomavirus (hpv) to cervical cancer. the authors conclude that over 90% of all cervical cancers can be attributed to certain hpv types-hpv 16 accounting for the largest proportion (roughly 50%) followed by hpv 18 (12%), hpv 45 (8%), and hpv 31 (5%). recognition of this circumstance has far-reaching implications for primary and secondary prevention of this malignancy. at present, prophylactic and therapeutic hpv vaccines are under deve ... | 1996 | 9041748 |
| workshop on screening for cancer of the uterine cervix in central america. | this article describes the objectives and content of a workshop held in managua, nicaragua, during november 1995, on screening for cervical cancer. the aims were to discuss cost-effective models of screening in countries with a high incidence of cervical cancer and to reach a consensus on principles for screening that is balanced with a country's resources. the workshop aimed to develop a planning framework and to identify program strengths and weaknesses by country. in 1990 there were 25,000 ... | 1996 | 9041753 |
| hybrid capture method for detection of human papillomavirus dna in clinical specimens: a tool for clinical management of equivocal pap smears and for population screening. | high- and intermediate-risk types of human papillomavirus (hpv) in concert with cofactors are responsible for over 90% of cervical cancers world-wide. while the pap smear is a valuable cancer prevention tool, its subjective nature leaves it error prone. as a result, hpv dna testing appears to be a needed adjunct to the pap smear. hybrid capture is a simple dna test with high specificity and sensitivity which appears useful for clinical management of equivocal pap tests and shows promise for mass ... | 1996 | 9037955 |
| intra-epithelial and invasive cervical neoplasia during hiv infection. | patients affected by human immunodeficiency virus (hiv) infection present an elevated risk of developing cancer. in the last 10 years, the relationship between human papilloma virus (hpv) infection and female cervical intra-epithelial neoplasia (cin) has been established. several studies have described an increased prevalence of both cervical hpv infection and cin among hiv-positive women compared to hiv-negative ones. a high recurrence rate of cin after standard treatment has been noted in hiv- ... | 1996 | 9038602 |
| national institutes of health consensus development conference statement: cervical cancer, april 1-3, 1996. national institutes of health consensus development panel. | the objective was to provide physicians and the general public with a responsible assessment of current screening, prevention, and treatment approaches to cervical cancer. | 1996 | 9023821 |
| cervical human papillomavirus infection and intraepithelial neoplasia: a review. | cervical human papillomavirus (hpv) infections and intraepithelial neoplasias are precursors to cervical cancer, the second most common cancer in women worldwide. hpv satisfies the epidemiologic criteria for causality; the role of other cofactors is under study. natural history studies show that most low-grade lesions (productive hpv infections) regress or persist, whereas high-grade lesions (those with integrated hpv dna) progress. immunobiologic studies demonstrate that infection peaks in the ... | 1996 | 9023824 |
| human papillomavirus biology. | 1996 | 9023844 | |
| detection of human viruses in periodontal pockets using polymerase chain reaction. | even though viruses have been implicated in the etiology of several medical and dental disorders, little or no data are available on the possible involvement of human viruses in the pathogenesis of human periodontal disease. this study investigated the presence of human cytomegalovirus, epstein-barr virus, herpes simplex virus, human papillomavirus and human immunodeficiency virus (hiv) in crevicular fluid samples from 30 patients with advanced periodontitis and 26 subjects with gingivitis. vira ... | 1996 | 9028252 |
| the current status of therapeutic hpv vaccine. | cervical carcinoma is the second most common cause of cancer-related deaths in women worldwide. recurrences occur in 15% of the patients after optimal treatment of low-risk early-stage disease. treatment results of recurrent disease are relatively poor and for this reason new therapeutic strategies are warranted. viral infection with human papillomavirus seems to have an essential part in the aetiology of cervical carcinoma. evidence for the assumption that cervical carcinoma, among other malign ... | 1996 | 9017105 |
| failure to demonstrate human papillomavirus (hpv) involvement in bowen's disease of the skin. | to assess the role of human papillomavirus (hpv) in the aetiology of extragenital bowen's disease (ebd), a series of 91 cases were analysed using in situ hybridization (ish) with whole genomic dna probes of hpv types 1, 2, 4, 6, 7, 11, 12, 15, 16, 18, 26, 36, 37, 39, 42, 55 and 59. no hpv dna was found in any of the samples tested. a polymerase chain reaction (pcr) was also used to analyse 37 of the 91 samples, using both the consensus primers my09/my11 which amplify a large number of hpv types ... | 1996 | 9017134 |
| expression of the major capsid protein of human papillomavirus type 11 in saccharomyces cerevisae. | the major capsid protein l1 of papillomaviruses expressed recombinantly or in infected cells has the intrinsic ability to form virus-like particles (vlps) which display conformational epitopes necessary to elicit high-titered, virus-neutralizing antibodies. we have shown previously that the l1 gene of human papillomavirus type 6a (hpv6) can be expressed efficiently in saccharomyces cerevisae (sc) as vlps. however, when we attempted to express the l1 gene cloned from the closely related hpv11 in ... | 1996 | 8973339 |
| the cervico-vaginal epithelium during 20 cycles' use of a combined contraceptive vaginal ring. | the aim of the study was to evaluate the influence of a combined contraceptive vaginal ring (ccvr) made of silastic on the cervico-vaginal epithelium during 20 cycles of use. a total of 76 volunteers used the ccvr releasing 0.120 mg etonogestrel and 0.015 mg ethinyloestradiol daily. cytological samples were taken of the vaginal epithelium, the ectocervix and the endocervix before the start, at 4 and 12 months, and at the end of the study. cytology, hormonal profiles, human papilloma virus (hpv) ... | 1996 | 8981130 |