Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| suppression of mycobacterium leprae-induced leucocyte migration inhibition following lepromin injection in healthy contacts of leprosy. preliminary observations. | 1985 | 3908863 | |
| vaccines against mycobacteria and other intracellular multiplying bacteria. | as the prototype of a vaccine against mycobacterial infection, the bcg (bacille bilié calmette et guérin) has been used against tuberculosis for more than 60 years. it is the only live attenuated vaccine used on humans in more than 182 countries or territories in the world, and very few changes have been made in its fabrication and distribution, except for the production of lyophilized seed-lots. however, its history is marked with controversies concerning its innocuity and efficacy. while bcg s ... | 1985 | 3909929 |
| production of monoclonal antibodies against mycobacterium leprae and armadillo-derived mycobacteria. | six monoclonal antibodies to mycobacterium leprae and armadillo-derived mycobacteria were produced. the monoclonal antibodies were characterized by an immunofluorescence assay using 22 mycobacterial strains. one monoclonal antibody, f47-21-3, reacted only with m. leprae; two, f45-9 and f45-15, reacted only with two armadillo-derived mycobacterial strains. these six monoclonal antibodies can be used for the identification of m. leprae and armadillo-derived mycobacteria. | 1985 | 3909933 |
| the allyl group for protection in carbohydrate chemistry. 17. synthesis of propyl o-(3,6-di-o-methyl-beta-d-glucopyranosyl)-(1----4)-o-(2,3- di-o-methyl-alpha-l-rhamnopyranosyl)-(1----2)-3-o-methyl-alpha- l-rhamnopyranoside: the oligosaccharide portion of the major serologically active glycolipid from mycobacterium leprae. | allyl 4-o-benzyl-alpha-l-rhamnopyranoside was converted into allyl 4-o-benzyl-3-o-methyl-alpha-l-rhamnopyranoside and this was condensed with 2,3,4-tri-o-acetyl-alpha-l-rhamnopyranosyl chloride to give a disaccharide derivative which was converted into allyl 4-o-benzyl-2-o-(2,3-o-isopropylidene-alpha-l-rhamnopyranosyl)-3-o-methyl -alpha- l-rhamnopyranoside. this disaccharide derivative was condensed with 2,4-di-o-acetyl-3,6-di-o-methyl-alpha-d-glucopyranosyl chloride to give a trisaccharide deri ... | 1985 | 3910287 |
| determination of antibodies in dried blood from earlobes of leprosy patients by enzyme-linked immunosorbent assay--a preliminary report. | an enzyme-linked immunosorbent assay (elisa) was used with soluble antigens of mycobacterium leprae. all blood samples collected from the earlobes of 109 leprosy patients and 100 healthy controls (from a non-endemic area of leprosy) were absorbed with m. vaccae, bcg, cardiolipin, and lecithin according to the technology of the fla-abs test before being tested in the elisa. the results (at a 1:200 blood dilution) showed that antibody activity gradually increased from tt to ll (mean od values: tt ... | 1985 | 3910746 |
| electron microscopic observations of acid-fast bacilli in nasal mucosal biopsies of lepromatous leprosy patients. | the fine structure of acid-fast bacilli in nasal mucosal lepromas of lepromatous leprosy patients was studied in ultrathin sections by the electron microscope. the cell envelope of the bacilli was the main point of these observations. the electron microscopic examination of some of the bacilli revealed the envelope to be multilaminate. some of the cellular matter was filled with an electron-less-dense, amorphous substance, surrounded with a membrane-like structure, and was adherent to the outerm ... | 1985 | 3910747 |
| an experimental study of the antileprosy activity of a series of thioamides in the mouse. | a series of substituted thioamides have been studied to establish whether their structure-activity pattern against mycobacterium leprae is similar to that displayed against m. tuberculosis. antileprosy activity was evaluated in the mouse foot pad using both the kinetic and continuous methods. ethionamide and prothionamide were found to be the most active compounds and to be of approximately equal potency. thioisonicotinamide was about five times less active. 2-t-butyl-thioisonicotinamide, 2-dime ... | 1985 | 3910748 |
| experimental reproduction of leprosy in seven-banded armadillos (dasypus hybridus). | four autochthonous seven-banded armadillos (dasypus hybridus) from argentina which developed disseminated leprosy are reported. the average time from inoculation to death was 14.6 months. acid-fast bacilli (afb) were found in the skin, nerves, spleen, lymph nodes, lungs, meninges, and striated muscle. several tests were used to prove that the afb found in the infected animals were mycobacterium leprae: a) failure to grow in standard culture media for mycobacteria, b) extraction of acid-fastness ... | 1985 | 3910749 |
| monoclonal antibodies and recombinant dna technology: present and future uses in leprosy and tuberculosis. | 1985 | 3910750 | |
| distribution of mycobacterium leprae in the circles of counting slides. | 1985 | 3910751 | |
| distribution of mycobacterium leprae in circular counting films. | 1985 | 3910752 | |
| recent trends in chemotherapy of leprosy. | 1985 | 3912375 | |
| the lack of therapeutic effects in mice of the combined gamma-irradiated mycobacterium leprae and viable bcg against mycobacterium leprae infection. | 1985 | 3913661 | |
| does the hansen bacillus harm or benefit the leprous patient? | 1985 | 3913662 | |
| primary dapsone resistance in leprosy. | 20 carefully selected untreated patients of bacilliferous leprosy were investigated for primary dapsone resistance by foot pad inoculation. mice were fed on 0.001 g% and 0.01 g% of dapsone during the period of study. mice in the control group were given normal rodent feed only. animals were sacrificed from 6 month onwards at 6 week intervals upto 9 months. in two animals the growth of m. leprae was not inhibited by 0.001 g% concentration of dapsone in diet, but was completely inhibited by 0.01 g ... | 1985 | 3913705 |
| dapsone resistance in north india. | an attempt has been made to screen the resistant strains of m. leprae in lepromatous patients from the eight northern states of india. by using the mouse foot pad technique it was found that in a total of 69 clinically suspected patients 33 (47.8%) harboured m. leprae with some degree of dapsone resistance. a detailed epidemiological study in these parts of the country may reveal the prevalence rate. | 1985 | 3913706 |
| levels of the third component of complement in mycobacterium leprae infected intact and immune compromised mice. | normal and immunosuppressed mice were infected with mycobacterium leprae and the bacillary counts were made from the footpads at 3, 6 and 9 months post inoculation. a decrease in the serum c3 level was observed in the infected groups of animals compared to controls. | 1985 | 3913707 |
| measurement of atp generation and decay in mycobacterium leprae in vitro. | the intracellular atp content of mycobacterium leprae isolated from armadillo tissue was approximately 1.5 x 10(-16) g per bacillus. during in vitro incubation of bacilli at 4 degrees c, 33 degrees c or 37 degrees c there was an exponential decrease in atp content, the rate depending on the medium and the temperature. m. leprae incorporated phosphate into atp and into other nucleotide materials during in vitro incubation. | 1985 | 3913745 |
| transmission of leprosy in nude mice. | nude mice in groups of 10 were exposed to m. leprae by subcutaneous injection and topically through the nose, lungs, mouth, stomach and skin, broken and unbroken. animals injected subcutaneously and those topically exposed to m. leprae through the nose developed localized disease which in the course of time became generalized. the nose seems to be the site of entry of m. leprae in this model. to the extent that these results can be generalized to humans exposed to m. leprae, it would seem that l ... | 1985 | 3914846 |
| effect of repeated lepromin testing on experimental nine-banded armadillo leprosy. | twenty-eight armadillos were lepromin tested and infected with m. leprae; 18 intravenously and 10 intradermally. the lepromin test was repeated after 3 months and at intervals of 6 months thereafter until their death or sacrifice up to 30 months. the one animal with tuberculoid lepromin was resistant and 14 of the 16 with lepromatous lepromin developed generalized disease. of the 11 with borderline lepromin, 6 developed disseminated disease and 5 were resistant. there is a definite relationship ... | 1985 | 3915005 |
| growth of mycobacterium leprae in a redox system: ii. further improvements in the system and growth efficiency. | improvement of the redox system for growth of m. leprae as brought about by modification in the concentration and mode of preparation of individual media constituents, and by addition of newer substances, is being reported. a structural modification in the construction of the thunberg's tubes and flasks that are used as culture vessels, has been introduced for ease of handling. vitamin e (alpha-tocopherol) has been found to be useful. concentrations of liposomes and gelatin in the medium could b ... | 1985 | 3915006 |
| computerized mathematical model of m. leprae population dynamics during multiple drug therapy. | a computerized mathematical model of m. leprae populations during multiple drug therapy (mdt) was constructed. relevant published information available to date was fed into it, and reasoned assumptions were made. from the model, it seems likely that mdt steadily selects bacteria resistant to the most powerful of the three drugs used: unless the individual bactericidal potencies of the drugs balance one another. if the drugs used have differing potencies, cure probably hinges on treatment being c ... | 1985 | 3915007 |
| studies on healthy contacts of leprosy patients--a preliminary report. | 91 healthy contacts of leprosy patients were studied for subclinical infection and possibly the pre-clinical stage of the disease using a battery of tests. it was observed that the test based on competitive inhibition of monoclonal antibody binding to the my2 a determinant of m. leprae identifies a preclinical stage of the disease. | 1985 | 3915008 |
| involvement of tongue in leprosy. | 1985 | 3915009 | |
| [immunological study of the easter island population to detect hansen's infection]. | 1985 | 3915139 | |
| [electron microscopic findings in peripheral nerve lesions of nude mouse inoculated with m. leprae--perineural lesion]. | 1985 | 3915747 | |
| [inoculation of mycobacterium leprae into beige mice]. | 1985 | 3916295 | |
| experimental leprosy in three species of monkeys. | eleven mangabey monkeys inoculated with mycobacterium leprae developed lepromatous-type leprosy. nine of the mangabeys were inoculated with m. leprae isolated from a mangabey with naturally acquired lepromatous leprosy. immune function was depressed in some of these animals after dissemination of the disease. two mangabeys developed lepromatous leprosy after inoculation with human m. leprae passaged in an armadillo. three rhesus and three african green monkeys inoculated with mangabey-derived m. ... | 1985 | 3917577 |
| hla class-ii immune response genes and products in leprosy. | 1985 | 3918314 | |
| fixation of mycobacterium leprae in unstained smears prior to shipment or storage: evaluation of formaldehyde using mycobacterium tuberculosis as indicator of efficacy. | 1985 | 3920468 | |
| antibody response to phenolic glycolipid i in inbred mice immunized with mycobacterium leprae. | the level of circulating antibody to phenolic glycolipid i of mycobacterium leprae was determined in 18 inbred strains of mice after immunization with m. leprae organisms. by using a solid-phase radioimmunoassay with phenolic glycolipid i as test antigen, a continuous distribution of antibody levels ranging from high to low was observed. the level was found to be controlled by multiple genes, including both h-2 complex- and igh allotype complex-linked genes. low antibody response to phenolic gly ... | 1985 | 3921463 |
| ultrastructural aspects of oral and facial lepromatous lesions. | the ultrastructure of 10 lepromatous lesions in the face and the palatal mucosa after different duration and length of treatment was studied. biopsies taken from patients who showed erythema nodosum leprosum (enl) revealed different ultrastructural characteristics from those taken from 'burnt out' and non-medicated cases. multiple secondary lysosomes were rarely seen in non-enl cases. the presence of mycobacterium leprae and an increased lysosomal activity in enl reactive cases is interpreted as ... | 1985 | 3921481 |
| detection of an antigen (my4) common to m. tuberculosis and m. leprae by 'tandem' immunoassay. | a novel 'tandem' immunoassay for the detection of mycobacterial antigen was devised using a monoclonal antibody (ml 34) both as solid phase 'capture' and as the 125i- or enzyme-labelled 'tracer' antibody. this antibody binds to the repeating epitopes (my4b) of a water-soluble protease-resistant antigen from m. tuberculosis, m. leprae and some other species of mycobacteria. optimal binding results could be obtained within 4 h by the consecutive incubation of ml34-coated microtitre plates with ant ... | 1985 | 3923119 |
| epidemiology of leprosy in relation to control. report of a who study group. | 1985 | 3925646 | |
| periodic acid-schiff reaction stains mycobacterium tuberculosis, mycobacterium leprae, mycobacterium ulcerans, mycobacterium chelonei (abscessus), and mycobacterium kansasii. | 1985 | 3925927 | |
| characterization of the specificity of monoclonal antibodies against mycobacterium tuberculosis by crossed immunoelectrophoresis. | the specificity of monoclonal antibodies against mycobacterium tuberculosis was determined by crossed immunoelectrophoresis. radiolabelled, non-precipitating monoclonal antibody was incorporated in the top gel together with unlabelled polyvalent, precipitating anti-bacillus calmette-guérin (bcg) antibody. inclusion of labelled antibody in individual precipitate lines was demonstrated by autoradiography. each monoclonal antibody was localized in a single precipitate line: antibody tb73 reacted wi ... | 1985 | 3927476 |
| a series of new antileprosy compounds derived from serotonin. | 1985 | 3927649 | |
| immunity to leprosy. ii. genetic control of murine t cell proliferative responses to mycobacterium leprae. | t cell proliferative responses to mycobacterium leprae were measured after immunization of mice at the base of the tail with antigen and challenging lymphocytes from draining lymph nodes in culture with m. leprae. this t cell response to m. leprae has been compared in 18 inbred strains of mice. c57bl/10j mice were identified as low responder mice. the congenic strains b10.m and b10.q were found to be high responders, whereas b10.br and b10.p were low responders. f1 (b10.m x c57bl/10j) and f1 (b1 ... | 1985 | 3928757 |
| an analysis of in vitro t cell responsiveness in lepromatous leprosy. | in lepromatous leprosy, there is extensive replication of mycobacterium leprae (m. leprae) within dermal macrophages. this lack of microbial resistance has been attributed to a defective cell-mediated immune response to m. leprae antigens. we have examined the in vitro response of t cells to m. leprae to determine if hyporesponsiveness could be reversed. the study included 40 unselected patients from new york and from colombia, most with the severe lepromatous form of the disease. we first noted ... | 1985 | 3928804 |
| temperature-induced changes in viability, diphenoloxidase and permeability of mycobacterium leprae. | among mycobacteria secretion of the enzyme diphenoloxidase has been established as a property of mycobacterium leprae. the antileprosy drug dapsone (dds), which completely inhibits the enzyme from plant and mammalian sources, does not readily penetrate intact m. leprae. when the drug is complexed with polylysine, it easily permeates the bacteria and produces 100% inhibition of its diphenoloxidase, suggesting a permeability barrier of the cytoplasmic membrane of m. leprae to dapsone. in this stud ... | 1985 | 3929029 |
| [towards a vaccine against leprosy]. | 1985 | 3933117 | |
| a possible metabolic role for o-diphenoloxidase in mycobacterium leprae. | among mycobacteria, mycobacterium leprae is unique in its ability to oxidize a variety of diphenols to quinones in vitro. what physiologic role o-diphenoloxidase has in the organism remained unknown. reducing substrates like nadph, nadh and ascorbic acid reacted with the quinone formed from dopa (3,4-dihydroxyphenylalanine); the substrates were oxidized and the quinone was reduced back to diphenol in the process. since the quinone undergoes reversible oxidation-reduction, diphenoloxidase might s ... | 1985 | 3935479 |
| leucocyte migration inhibition response of mycobacterium habana with sensitized animals and cells from leprosy patients. | response of mycobacterium habana sensitized mouse cells against habanin (sonicated extract) lepromin (dharmendra) and tuberculin has been studied in the leucocyte migration inhibition test. the homologous antigenic response has evoked maximum inhibition as compared to heterologous antigens but close antigenic association has also been observed with lepromin and tuberculin. with human cells from leprosy patients these antigens have evoked analogous response indicating cross immunogenicity of m. h ... | 1985 | 3935726 |
| the role of interleukin-2 (il-2) in the specific unresponsiveness of lepromatous leprosy to mycobacterium leprae: studies in vitro and in vivo. | the role of il-2 in the immunological deficiency of lepromatous leprosy patients towards mycobacterium leprae have been studied further. after initial stimulation with m. leprae + il-2, lepromatous lymphocytes could be restimulated with m. leprae alone. the specificity of the responses obtained varied. some patients gave a stronger response to bcg as compared to m. leprae, while in others a stronger response to m. leprae as compared to bcg was obtained. studies of the composition of lymphocytes ... | 1985 | 3936783 |
| epidemiology of leprosy in relation to control (who technical report series no. 716 of 1985). part i. | 1985 | 3938985 | |
| a culture medium for cultivation of mycobacteria, probably mycobacterium leprae from mycobacterium leprae infected tissues. | mycobacterium leprae suspensions were prepared from infected armadillos. the m. leprae cells were inoculated into culture media containing kh2po4 4.7. g. na2hpo4 2 g, sodium thioglycolate 1 g, (nh4)2so4 2 g, mgso4 0.1 g, ferric ammonium citrate 0.05 g, and lipoic acid (thioctic acid) 0.1 g in one liter distilled water. the solution was enriched with heat killed, sonicated leprosy derived mycobacterium x or crude mycobactin extract from m. phlei to contain + 0.2 micrograms mycobactin per 1 ml in ... | 1985 | 3938986 |
| [lysosome and leprosy bacilli]. | 1985 | 3939304 | |
| cross-reactivity of antigens from the cytoplasm and cell walls of some corynebacteria and mycobacteria. | leprosy-derived corynebacteria (ldc) are non-acid-fast organisms isolated from leprosy lesions in humans. in this study 20 antigens of native ldc cytoplasm were identified by immunoelectrophoresis, and autoclaving yielded the m1 component, which strongly cross-reacted with antigen 60 of mycobacterium bovis bcg (bacille calmette-guérin) and antigen 7 of mycobacterium leprae. the polysaccharide moiety of m1 was immunologically related to the ldc cell wall polysaccharide previously characterized as ... | 1985 | 3965589 |
| the mononuclear phagocyte system in granulomas. | a comparison is made between immunologically induced and non-immunologically induced granulomas in guinea-pigs injected with metals (zirconium and aluminum) or with mycobacteria (bcg vaccine and mycobacterium leprae). immunological granulomas were characterized by epithelioid cells and fibrosis, whereas non-immunological granulomas contained phagocytosing macrophages with little evidence of fibroblast activation. epithelioid cells carry the same specific macrophage antigen as phagocytosing macro ... | 1985 | 4015981 |
| biochemical alterations in the serum of armadillos (dasypus novemcinctus) infected with mycobacterium leprae. a preliminary report. | armadillos (dasypus novemcinctus) were inoculated with mycobacterium leprae isolated from lepromas taken from untreated lepromatous patients or from the spleen of an armadillo previously infected with human m. leprae. the effect of the infection on the serum levels of lactic dehydrogenase (ldh), alkaline phosphatase (alkp), glutamate-oxalacetate (got) and glutamate-pyruvate (gpt) transaminases was investigated. in general, there was a good correlation between positive evidences of infection and ... | 1985 | 4020215 |
| inappropriate responses to mycobacterium leprae infections--c reactive protein in man and serum amyloid p in mice. | in a study of c-reactive protein (crp) levels in the sera of 77 patients with leprosy, it was found that in the majority of newly diagnosed patients, the level was within the normal range for a healthy malaysian population. elevated levels did occur, but were usually found in patients with complications, and were more likely to occur in patients who had been receiving drug treatment for some time. this suggested that mycobacterium leprae infection by itself does not stimulate crp synthesis and c ... | 1985 | 4042426 |
| purification of phenolic glycolipid i from armadillo and human sources. | 1985 | 4045269 | |
| isolation of phenolic glycolipid i from human lepromatous nodules. | 1985 | 4045270 | |
| leprosy in children one year of age and under. | information obtained from a review of the literature, the united states armed forces institute of pathology files, and from a correspondence survey revealed a total of 91 infants one year of age and under in whom leprosy was diagnosed. biopsy confirmation was available on 19 infants, and in an additional 32 patients the diagnosis of leprosy was considered certain even though biopsy confirmation was not obtained. although the mother was probably the most common source of the infection (29 infants ... | 1985 | 4086915 |
| histology of the mitsuda reaction of healthy adults with no known contacts with leprosy patients. | mitsuda tests were performed on 100 healthy adult brazilian males unrelated consanguineously to leprosy patients and with no known contacts with this disease. three lepromatous patients served as negative mitsuda controls. the lepromin employed was from mycobacterium leprae-infected armadillo tissue prepared according to who standards. clinical response to the lepromin inoculation was graded according to the recommendations of the vi international leprosy congress held in madrid (1953). the hist ... | 1985 | 4086917 |
| a single skin lesion--an unusual presentation of lepromatous leprosy. | lepromatous leprosy presenting as a solitary lesion with a high bacterial count is a rare occurrence. such a case has been followed at the national hansen's disease center, carville, louisiana, u.s.a., since 1977. the lesion was located on the left elbow and had been present for about nine months. the bacterial index in the lesion was 5+ and the morphological index was 0%, but slit-skin smears elsewhere were negative. the histopathology of the lesion was that of subpolar lepromatous leprosy, and ... | 1985 | 4086919 |
| demonstration of mycobacterium leprae specific antigens in leprosy lesions using monoclonal antibodies. | cryostat sections of dermal lesions from 13 untreated patients of leprosy were studied by indirect immunoperoxidase using monoclonal antibodies (mlo4 & mlo6), defining m. leprae specific antigens. the lymphocytes and macrophages in both the tuberculoid and lepromatous granulomas showed membranous staining with the above antibodies. m. leprae organisms in the lepromatous granulomas and the cells in the section of lymph nodes of patients with tuberculosis, or sections of normal skin or psoriatic l ... | 1985 | 2416852 |
| results of a world health organization-sponsored workshop on monoclonal antibodies to mycobacterium leprae. | 1985 | 2580798 | |
| immunoassays of field isolates of mycobacterium bovis and other mycobacteria by use of monoclonal antibodies. | antigen extracts obtained by sonication of 22 strains of mycobacterium bovis from cattle and badgers together with extracts of strains of m. tuberculosis, m. paratuberculosis, m. avium, m. africanum, m. kansasi, m. leprae and bcg were examined with a panel of 10 monoclonal antibodies to m. tuberculosis or m. leprae. antigen extracts were coated in aqueous solution (wet coating) and the extracts were also dried on to the polyvinyl plates (dry coating). when dry coating was compared to wet coating ... | 1985 | 2409287 |
| monoclonal antibodies to a 28,000 mol. wt protein antigen of mycobacterium leprae. | monoclonal antibodies (moab) have been used to analyse a protein antigen from mycobacterium leprae with a subunit mol. wt of 28,000 daltons. three different patterns of species specificity were observed with two antibodies being specific for m. leprae, two partially specific, and one broadly cross-reactive amongst all mycobacteria. competitive binding and sandwich assays demonstrated that the specific and partially specific antibodies recognized closely related regions of the molecule while the ... | 1985 | 2410167 |
| quality control of skin smear services in leprosy programmes: preliminary experience with inter-observer comparison in routine services. | 1985 | 2414625 | |
| a spot test for detection of antibodies to phenolic glycolipid i. | 1985 | 2414626 | |
| mycobacterium leprae antigens involved in human immune responses. i. identification of four antigens by monoclonal antibodies. | four distinct antigens were identified in soluble sonicates of mycobacterium leprae by using a panel of 11 monoclonal antibodies. cross-reactivity studies with other mycobacterial species were conducted by using elisa and immunoblot assays, and demonstrated that determinants on two of the antigens were present in many mycobacteria, whereas the other two were limited in distribution. competitive inhibition experiments with radiolabeled monoclonal antibodies showed cross-inhibition between antibod ... | 1985 | 2415603 |
| multi-drug therapy of leprosy. | 1985 | 2420903 | |
| an elisa-inhibition test using monoclonal antibody for the serology of leprosy. | in this study a mouse monoclonal antibody (47-9) is described, which recognized an epitope on the 36 kd protein antigen of m. leprae. the monoclonal antibody showed specificity for m. leprae. an elisa-inhibition test based on the competitive inhibition by antibodies from human test sera of the binding of the enzyme labelled monoclonal antibody to m. leprae was developed. seropositivity was found in 100% of the multibacillary leprosy patients group and in 91% of the paucibacillary patients. only ... | 1985 | 2417764 |
| characterization of the immune response to an epitope on mycobacterium leprae antigen 7 defined by a monoclonal antibody. | a mouse monoclonal antibody (038d-c6) was shown by crossed immunoelectrophoresis and radioimmunoassay to react with an epitope on the mycobacterium leprae antigen 7. this epitope was highly crossreactive with bcg/m. tuberculosis and of a non-arabinogalactan-arabinomannan nature. a solid-phase radioimmunoassay (spria) was applied, based on competitive inhibition by human sera of antigen binding by this anti-m. leprae monoclonal antibody. inhibitory activity determined by this assay decreased mark ... | 1985 | 2418496 |
| biological, chemical, immunological and staining properties of bacteria isolated from tissues of leprosy patients. | two kinds of microorganisms are found in tissue of leprosy patients: mycobacterium leprae (ml) and leprosy derived corynebacteria (ldc). ml from untreated patients has an alcohol-acid-fastness, which is lost upon treatment with antibiotics and immune response (tuberculoid leprosy). vulnerable ml thus produced can be reversibly de-stained by organic solvent: in tissue sections from tuberculoid and treated patients, more bacteria are, thus, revealed by the wade-fite than by the ziehl-neelsen proce ... | 1985 | 2429862 |
| [leprous symmetric keratoderma palmaris et plantaris. apropos of 2 clinical cases]. | two cases of "keratoderma palmaris et plantaris symmetric leprous" are described, which rarely occur in the field of acrokeratosis, contracted by infection. | 1985 | 2977127 |
| ultrastructure of leprous phlebitis. | an ultrastructural study on vein biopsies from six lepromatous leprosy patients was carried out. the results showed that a) the lumen-lining bacillated cells were endothelial in origin due to the presence of specific weibel-palade endothelial cell granules; b) endothelial cells released mycobacterium leprae into the lumen by exophagocytosis; c) m. leprae were able to grow and multiply in the endothelial and smooth muscle cells; and d) smooth muscle cells did not show any evidence of reaction due ... | 1985 | 3003213 |
| respiration in mycobacterium leprae. | fairly pure leprosy bacilli were easily collected from nude mouse foot pad lepromas by the ficoll density gradient centrifugation and alkali treatment methods. the yield of bacilli available for biochemical study was 42.6%. the density of mycobacterium leprae was very heterogeneous. the percent of solid bacilli in the light bacilli fraction was 23%; that in the heavy bacilli fraction was 40%. the endogenous respiration activity in the heavy bacilli was greater than that in light bacilli. the ave ... | 1985 | 3003214 |
| genes for the protein antigens of the tuberculosis and leprosy bacilli. | the lambda gt 11 expression vector permitted us to survey protein antigens of mycobacterium leprae and mycobacterium tuberculosis expressed in escherichia coli. using monoclonal antibodies, recombinant clones were detected producing three major antigens of m. tuberculosis and five major protein antigens of m. leprae. these recombinant antigens produced in e. coli should prove useful for diagnosis, epidemiology and possibly the development of recombinant mycobacterial vaccines. | 1985 | 3006821 |
| the influence of immunosuppression and immunodeficiency on infections with leprosy and tuberculosis. | 1985 | 2987376 | |
| hydrogen peroxide and superoxide production by peripheral blood monocytes in leprosy. | susceptibility to infection with mycobacterium leprae, the causative organism of leprosy, is the result of a defect in cell-mediated immunity (cmi). the co-operation of macrophages and t lymphocytes is known to be essential for competent cmi response. in this study we have examined peripheral blood monocytes from a range of leprosy patients in an attempt to identify a possible defect in macrophage function. the ability of these cells to produce hydrogen peroxide and superoxide, two bactericidal ... | 1985 | 2988833 |
| human monoclonal antibodies against mycobacterium leprae. | human hybridomas were constructed which produce antibodies against three different extracts of mycobacterium leprae. a thioguanine-resistant (thgr), ouabain-resistant (ouar), human lymphoblastoid cell line, kr-4, was hybridized with epstein-barr virus-transformed cell lines from lepromatous leprosy patients with fusion frequencies of greater than 10(-5). non-epstein-barr virus-transformed donor cells fused at much lower rates (less than 2 x 10(-7]. hybrids were selected in medium containing hypo ... | 1985 | 2989177 |
| defective cell-mediated immunity in leprosy: failure of t cells from lepromatous leprosy patients to respond to mycobacterium leprae is associated with defective expression of interleukin 2 receptors and is not reconstituted by interleukin 2. | patients with lepromatous leprosy (ll) but not borderline tuberculoid leprosy (bt) have defective cell-mediated immune responses to mycobacterium leprae, despite normal responses to other stimuli, as judged by in vivo skin testing and in vitro lymphocyte transformation. to investigate the basis of the immune defect in ll patients, we studied the ability of patient mononuclear leukocytes to produce interleukin 1 (il 1) and interleukin 2 (il 2) upon stimulation with m. leprae, and determined the a ... | 1985 | 2989366 |
| activation of human monocytes in leprosy. | in leprosy, the common etiologic agent is the same mycobacterium leprae, but the clinical manifestations are various, including the tuberculoid and lepromatous types. in tuberculoid type leprosy, macrophages in the granuloma differentiate into epithelioid cells; in the lepromatous type, in contrast, they differentiate into lepra cells containing multiple m. leprae. thus host factors, which regulate macrophage activities, determine the type of leprosy. to understand such regulation of macrophage ... | 1985 | 2989662 |
| evidence for the activity of rifampin on the neuropathy of foot pad-inoculated mice with mycobacterium leprae. | 1985 | 2995517 | |
| epstein-barr virus-transformed b cell lines present m. leprae antigens to t cells. | we have investigated whether or not epstein-barr virus-transformed lymphoblastoid b cell lines (ebv-blcl) are able to present mycobacterium leprae (m. leprae) to antigen-reactive t cell lines and clones. such ebv-blcl would provide us with a homogeneous and unlimited source of antigen-presenting cells. antigen-triggered proliferation of t cells has been studied with co-cultures either with autologous or allogeneic irradiated ebv-blcl. our results show that ebv-blcl are able to present m. leprae ... | 1985 | 3001928 |
| [preliminary observation on experimental infection with mycobacterium leprae in hedgehogs (erinaceus europaeus linne')]. | 1985 | 2938761 | |
| [preliminary observation on the effect of thymic peptide in the experimental infection of mice with mycobacterium leprae]. | 1985 | 2939979 | |
| [detection of antibodies in blood from the ear lobes of leprosy patients by enzyme-linked immunosorbent assay--a preliminary report]. | 1985 | 2939997 | |
| natural emergence of antigen-reactive t cells in lepromatous leprosy patients during erythema nodosum leprosum. | fifteen lepromatous leprosy (ll) patients undergoing erythema nodosum leprosum (enl) reactions were compared with 13 stable, uncomplicated, anergic individuals of the same leprosy background. enl patients showed significant antigen-induced leukocyte migration inhibition (migration index = 0.058 +/- 0.01), paralleling the values obtained with a responder tuberculoid leprosy population (migration index = 0.04 +/- 0.004). both phytohemagglutinin-induced general t-cell proliferation and, more signif ... | 1985 | 2933339 |
| host-parasite interaction in relation to leprosy. | 1985 | 2934487 | |
| cellular immunity in lepromatous and tuberculoid leprosy. | the depression of cellular immunity in lepromatous patients is not understood. while the blood monocytes of leprosy patients appear to be activated normally by lymphokines, t cell proliferation and production of lymphokines in response to mycobacterium leprae are impaired in lepromatous patients. attempts to restore responsiveness in cells from these patients have been unsuccessful in our hands. the addition of exogenous il-2 to leukocyte cultures does not appear to restore responsiveness to m. ... | 1985 | 2935494 |
| interleukin-2-induced t-cell response to m. leprae in lepromatous leprosy: reversion of a suppressor mechanism or expansion of a small m. leprae-reactive t-cell pool? | 1985 | 2935587 | |
| monoclonal antibodies against mycobacterial antigens. | the world health organization (who) scientific working groups on the immunology of leprosy (immlep) and tuberculosis (immtub) recently organized two international workshops designed to characterize the specificity and reaction patterns of approximately 55 murine monoclonal antibodies (mabs) which had been raised against mycobacterium leprae or mycobacterium tuberculosis and submitted to the immlep and immtub monoclonal antibody banks. | 1985 | 25291216 |
| [histopathological investigation of the central nervous system in leprosy. i. distribution of para-rosanilin positive materials in leprosy with special relation to tissue injury]. | 1984 | 6085732 | |
| ultrastructural and histophysiological studies on the blood-nerve barrier and perineurial barrier in leprosy neuropathy. | onset and nature of ultrastructural changes in endoneurial vasa nervorum during the pathogenesis of leprosy neuropathy and possibly associated alterations in the "blood-nerve barrier" were investigated, together with perineurial barrier functioning, in mice infected 20-28 months previously with mycobacterium leprae and in (ageing) non-infected mice. barriers were tested by i.v. administration of markers (trypan blue and ferritin) 1-4 days before killing the mice. twenty-eight months after infect ... | 1984 | 6095579 |
| study of the involvement of the sciatic nerve following inoculation with m. leprae and other mycobacteria in the mouse foot pad. | in order to determine whether mycobacterium leprae alone produce the typical damage in the sciatic nerves of foot pad inoculated mice as demonstrated earlier, a comparative study was undertaken using various other mycobacteria inoculated into the hind foot pads of normal swiss white mice. the findings indicate that fmr isolates no. 51 and no. 75 and m. avium showed multiplication in the foot pads of the mice throughout the 4th, 6th or 8th postinoculation months and these infections were associat ... | 1984 | 6100185 |
| leprosy 1984. | 1984 | 6143080 | |
| metabolism in mycobacterium leprae: its relation to other research on m. leprae and to aspects of metabolism in other mycobacteria and intracellular parasites. | recently, some knowledge of metabolic pathways, rather than individual enzyme activities of m. leprae, is becoming available. ultimately this may be useful in devising culture media for m. leprae. knowledge restricted to individual reactions may be misleading. for instance, the detection of glcnacase and beta-glucuronidase and the subcellular localization of hyaluronic acid led to attempts to cultivate m. leprae on hyaluronic-acid based medium. subsequent investigations suggested that there was ... | 1984 | 6144638 |
| ansamycin activity against rifampicin-resistant mycobacterium leprae. | 1984 | 6144865 | |
| enumeration of purified suspensions of mycobacterium leprae. | previously described methods of counting noncultivable bacteria have a number of drawbacks including unpredictable variation due to differential staining, low reproducibility between replicate sample smears, and inexact estimations of the bacillary population due to the non-normal distribution of bacilli across the counting field. a simple method is described which minimizes the disadvantages of previous methods and allows the application of a stratified sample technique which improves the accur ... | 1984 | 6200451 |
| analysis of the major antigenic determinants of the characteristic phenolic glycolipid from mycobacterium leprae. | antibodies to the major phenolic glycolipid purified from mycobacterium leprae have been demonstrated previously in sera of leprosy but not tuberculosis patients using an elisa. the major antigenic determinants on this molecule were investigated using antisera raised in rabbits to the purified glycolipid and with a pool of sera from human lepromatous leprosy patients. a small, but significant cross-reaction was observed with the glycolipids from m. bovis and m. kansasii, which contain the phenol ... | 1984 | 6201310 |
| staining tissue-derived mycobacterium leprae with fluorescein diacetate and ethidium bromide. | a fluorescent staining procedure incorporating the use of fluorescein diacetate (fda) and ethidium bromide (eb) has previously been shown to accurately measure the viability of saprophytic mycobacterial cells. green-stained cells were shown to be viable and red-stained cells, dead. staining mycobacterium leprae cells with fda/eb, however, was complicated by interfering tissue components which masked the presence of stained bacteria. a petroleum ether separation technique enables m. leprae to be ... | 1984 | 6202650 |
| skin testing with mycobacterial reagents in leprosy. | 1984 | 6203199 | |
| use of an artificial antigen containing the 3,6-di-o-methyl-beta-d-glucopyranosyl epitope for the serodiagnosis of leprosy. | the coupling of synthetic 3,6-di-o-methyl-beta-d-glucopyranosyl-(1----4)-2,3-di-o-methyl-alpha-l -rhamnopyranose, the hapten determinant of phenolic glycolipid i from mycobacterium leprae, to bovine serum albumin (bsa) by reductive amination produced the antigen epsilon-n-1-[1-deoxy-2,3-di-o-methyl-4-o-(3',6'-di-o-methyl-beta -d-glucopyranosyl)-rhamnitol]-lysyl-bsa, which proved highly sensitive in elisa and showed good concordance with the native glycolipid in analysis of serum samples from 223 ... | 1984 | 6207246 |
| immunological unresponsiveness in leprosy. | the various forms of leprosy form a clinical and immunological spectrum which offers extraordinary possibilities for insight into immunoregulatory mechanisms in man. at one pole, tuberculoid leprosy, patients develop high levels of cell-mediated immunity which ultimately results in killing of bacilli in the tissues, albeit often with damage to nerves. at the lepromatous pole, patients exhibit selective immunological unresponsiveness to antigens of m. leprae. even though all currently known prote ... | 1984 | 6208143 |
| [staining methods used in the identification of mycobacterium leprae. historical review]. | 1984 | 6209782 | |
| toxic effect of the peroxidase-hydrogen peroxide-halide antimicrobial system on mycobacterium leprae. | mycobacterium leprae are killed by myeloperoxidase (or eosinophil peroxidase), h2o2, and a halide, thus suggesting a mechanism for their destruction by peroxidase-containing phagocytes. | 1984 | 6325350 |