Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| gern, an antiporter homologue important in germination of bacillus cereus endospores. | a homologue of the grma spore germination gene of bacillus megaterium and of a nah-antiporter gene (napa) of enterococcus hirae has been identified in bacillus cereus 569 (atcc 10876). the putative protein product has 58 and 43% amino acid identity with grma and napa, respectively. insertional inactivation of this b. cereus gene, named gern, did not affect vegetative growth or sporulation. the null mutant spores were 30-fold slower to germinate in inosine (5 mm) but germinated almost normally in ... | 2001 | 11133940 |
| sensitivities of germinating spores and carvacrol-adapted vegetative cells and spores of bacillus cereus to nisin and pulsed-electric-field treatment. | treatment of bacillus cereus spores with nisin and/or pulsed-electric-field (pef) treatment did not lead to direct inactivation of the spores or increased heat sensitivity as a result of sublethal damage. in contrast, germinating spores were found to be sensitive to pef treatment. nisin treatment was more efficient than pef treatment for inactivating germinating spores. pef resistance was lost after 50 min of germination, and not all germinated spores could be inactivated. nisin, however, was ab ... | 2001 | 11282623 |
| cytk toxin of bacillus cereus forms pores in planar lipid bilayers and is cytotoxic to intestinal epithelia. | cytk is a cytotoxin isolated from a strain of bacillus cereus cultured from cases of necrotic enteritis and the amino acid sequence of the protein suggests that it may belong to the family of beta-barrel pore-forming toxins. we show here in planar lipid bilayers the toxin is able to form pores which are weakly anion selective and exhibit an open channel probability close to one. the predicted minimum pore diameter is approximately 7 a. we also show that cytk is a potent cytotoxin against human i ... | 2001 | 11287145 |
| influence of carvacrol on growth and toxin production by bacillus cereus. | the natural antimicrobial compound carvacrol was investigated for its effect on diarrheal toxin production by bacillus cereus. carvacrol (0-0.06 mg/ml) reduced the viable count and the maximal specific growth rate (mumax) of b. cereus in bhi broth. the total amount of protein was not affected by carvacrol. however, a sharp decrease (80%) in diarrheal toxin production was observed in the presence of 0.06 mg/ml carvacrol. carvacrol also inhibited toxin production in soup, but approximately 50-fold ... | 2001 | 11294360 |
| diacylglycerol effects on phosphatidylinositol-specific phospholipase c activity and vesicle fusion. | diacylglycerol increased the hydrolytic activity of phosphatidylinositol-specific phospholipase c on large unilamellar vesicles containing 5-40% phosphatidylinositol. moreover, diacylglycerol increased the rate and extent of vesicle fusion (contents mixing) induced by the enzyme. kinetic studies of intervesicular lipid mixing revealed that fusion was limited by the frequency of contacts involving two diacylglycerol-rich domains. | 2001 | 11297746 |
| biosynthesis of phb tercopolymer by bacillus cereus uw85. | the study was attempted to determine the ability of a gram-positive bacillus cereus uw85 strain to biosynthesize poly (3-hydroxybutyrate) copolymers when epsilon-caprolactone, or epsilon-caprolactone and glucose, were used as carbon sources. | 2001 | 11298229 |
| thuricin 7: a novel bacteriocin produced by bacillus thuringiensis bmg1.7, a new strain isolated from soil. | detection and identification of new antagonistic activities towards bacillus cereus and relatives. | 2001 | 11298934 |
| the effect of growth stage and growth temperature on high hydrostatic pressure inactivation of some psychrotrophic bacteria in milk. | the effect of high hydrostatic pressure on the survival of the psychrotrophic organisms listeria monocytogenes, bacillus cereus, and pseudomonas fluorescens was investigated in ultrahigh-temperature milk. variation in pressure resistance between two strains of each organism were studied. the effect of growth stage (exponential and stationary phase), growth temperature (8 and 30 degrees c) on pressure resistance, and sublethal pressure injury were investigated. exponential-phase cells were signif ... | 2001 | 11307889 |
| psychrotolerant species from the bacillus cereus group are not necessarily bacillus weihenstephanensis. | twenty-six strains of bacillus cereus from different sources were determined to be either mesophilic or psychrotrophic by growth at 6 and 42 degrees c. the strains were also screened by two polymerase chain reaction (pcr) methods designed to discriminate between mesophilic and psychrotrophic types. seventeen of the 26 strains were able to grow at 6 degrees c, but only four conformed to the new psychrotolerant species bacillus weihenstephanensis. among the 26 strains were two which caused outbrea ... | 2001 | 11313138 |
| evaluation of an automated culture system for detecting bacterial contamination of platelets: an analysis with 15 contaminating organisms. | approximately 1 in 2000 platelet components are bacterially contaminated. the time to detection of 15 seeded organisms in platelets recovered from an automated culture system was studied. | 2001 | 11316897 |
| bacillus cereus bacteremia and meningitis in immunocompromised children. | two cases of bacillus cereus meningitis in immunocompromised children at our hospital within a 2-month period prompted us to review b. cereus--related invasive disease. we identified 12 patients with b. cereus isolated in blood cultures from september 1988 through august 2000 at our institution. three of these patients also had b. cereus isolated from csf specimens; 1 additional patient had possible cns involvement (33%, group a), whereas 8 patients had no evidence of cns involvement (67%, group ... | 2001 | 11317247 |
| a general strategy for identification of s-layer genes in the bacillus cereus group: molecular characterization of such a gene in bacillus thuringiensis subsp. galleriae nrrl 4045. | despite its possible role in virulence, there has been little molecular characterization of members of the s-layer protein family of the bacillus cereus group. it is hypothesized that the components of the s-layers are likely to display similar anchoring structures in bacillus thuringiensis and bacillus anthracis. based on inferred sequence similarities, a dna fragment encoding the cell-wall-anchoring domain of an s-layer component of bac: thuringiensis subsp. galleriae nrrl 4045 was isolated. t ... | 2001 | 11320137 |
| foodborne diseases in kenya. | to determine the occurrence of foodborne disease outbreaks in kenya and the efforts employed to combat them. | 2001 | 11320765 |
| surface characteristics of bacillus cereus and its adhesion to stainless steel. | the ability of a bacillus cereus strain, isolated from spoiled milk, to adhere to the surface of stainless steel chips was evaluated during its growth in diluted tryptic soy broth (dtsb). the number of cells that adhered to the surface increased markedly as the culture reached the end of the log phase and entered stationary phase, and continued to increase with further incubation. the surface properties of cells from the log, stationary, and late stationary phases were measured by hydrophobic in ... | 2001 | 11322692 |
| familial mutations and zinc stoichiometry determine the rate-limiting step of nitrocefin hydrolysis by metallo-beta-lactamase from bacteroides fragilis. | the diverse members of the metallo-beta-lactamase family are a growing clinical threat evolving under considerable selective pressure. the enzyme from bacillus cereus differs from the bacteroides fragilis enzyme in sequence, zinc stoichiometry, and mechanism. to chart the evolution of the more reactive b. fragilis enzyme, we have made changes in an active site cysteine residue as well as in zinc content to mimic that which occurs in the b. cereus enzyme. specifically, by introducing a c104r muta ... | 2001 | 11327823 |
| involvement of the arg-asp-his catalytic triad in enzymatic cleavage of the phosphodiester bond. | phosphatidylinositol-specific phospholipase c (pi-plc) catalyzes the cleavage of the p-o bond in phosphatidylinositol via intramolecular nucleophilic attack of the 2-hydroxyl group of inositol on the phosphorus atom. our earlier stereochemical and site-directed mutagenesis studies indicated that this reaction proceeds by a mechanism similar to that of rnase a, and that the catalytic site of pi-plc consists of three major components analogous to those observed in rnase a, the his32 general base, ... | 2001 | 11331006 |
| microbiological status of commercially available medicinal herbal drugs--a screening study. | one hundred and thirty-eight medicinal herbal drugs obtained from different suppliers were examined for microbial contaminants and for the detectability of pathogenic microorganisms. for this purpose, several microbiological standard parameters (total aerobic mesophilic count, enterobacteria, coliforms, aerobic sporeformers, yeasts and moulds, enterococci, lactobacilli, pseudomonades and aeromades) and selective methods for the detection of indicator microorganisms and pathogens (e. coli, entero ... | 2001 | 11345700 |
| exogenous phospholipase c stimulates epithelial cell migration and integrin expression in vitro. | phospholipase c secreted by bacterial pathogens has been identified as a virulence factor in several human diseases and has been implicated in impeding wound healing. the role of phospholipase c in the intracellular signal control of epithelial growth was studied in normal human skin keratinocytes cultured in conditions simulating aspects of wound healing. bacillus cereus phospholipase c decreased cell-cell contact and increased cell migration resulting in disruption of the advancing epithelial ... | 2001 | 11350646 |
| fluoranthene-2,3- and -1,5-diones are novel products from the bacterial transformation of fluoranthene. | fluoranthene is one of the predominant compounds found in soils and sediments contaminated with polycyclic aromatic hydrocarbons (pah). four bacterial strains isolated from pah-contaminated soils transformed fluoranthene to a number of products during growth on phenanthrene, including the novel metabolites fluoranthene-2,3-dione (f23q) and fluoranthene-1,5-dione (f15q). given the known toxicity and mutagenicity of f23q, we focused on characterizing this metabolite with respect to its effects on ... | 2001 | 11351535 |
| inhibition tests for detection and presumptive identification of tetracyclines, beta-lactam antibiotics and quinolones in poultry meat. | a combination of three plates, seeded with strains of micrococcus luteus, bacillus cereus or escherichia coli, can be used for detection of residues of beta-lactam antibiotics, tetracyclines and fluoroquinolones. the sensitivity of each plate is optimal for only one of these groups, resulting in detection limits (lod) lower than the corresponding maximum residue limits (mrl) and in distinct inhibition patterns typical for each antibiotic family. beta-lactam antibiotics such as penicillin g, ampi ... | 2001 | 11358180 |
| the expanding spectrum of disease caused by bacillus cereus. | 2001 | 11368114 | |
| changes in the disintegration properties of some brands of paracetamol tablets inoculated with four bacterial species. | four most common brands of paracetamol (4-aceta-midophenol) tablets were examined for the changes in their disintegration properties after inoculation with staphylococcus aureus, bacillus cereus, klebsiella aerogenes and pseudomonas aeruginosa and incubating for 5 weeks. the disintegration times varied from one brand to the other, reaching maximum values of 72 min., 82 min., 110 min. and 120 min. for s. aureus, b. cereus, p. aeruginosa and klebsiella aerogenes, respectively. all brands of parace ... | 2001 | 11370281 |
| a novel surfactant nanoemulsion with a unique non-irritant topical antimicrobial activity against bacteria, enveloped viruses and fungi. | a novel non-ionic surfactant nanoemulsion designated 8n8 has been tested for its biocidal activity. one percent 8n8 produced effective bactericidal activity against bacillus cereus, bacillus subtilis, haemophilus influenzae, neisseria gonorrhoeae, streptococcus pneumoniae, and vibrio cholerae in 15 minutes. in contrast, most enteric gram-negative bacteria were resistant to 8n8. one percent 8n8 was also virucidal within 15 minutes for all tested enveloped viruses, including herpes simplex type 1, ... | 2001 | 11372645 |
| field evaluation of a bioregulator containing live bacillus cereus spores on health status and performance of sows and their litters. | the efficacy of paciflor, a bioregulator containing live bacillus cereus cip 5832 spores, was assessed in sows during late pregnancy and lactation, as well as in their piglets up to the growing phase. two groups each of 30 pregnant gilts and sows received normal feed (t1 group), or feed with 85 g paciflor per ton feed (t2 group), from 15 days prior to farrowing up to the end of the lactation period. furthermore, 15 litters of the t1 group and 15 litters of the t2 group, were offered normal feed ... | 2001 | 11379387 |
| phospholipases stimulate secretion in rbl mast cells. | roles for glycerophospholipids in exocytosis have been proposed, but remain controversial. phospholipases are stimulated following the activation of the high-affinity receptor for immunoglobulin e (ige) in mast cells. to study the biochemical sequelae that lead to degranulation, broken cell systems were employed. we demonstrate that the addition of three distinct types of exogenous phospholipases (i.e., bcplc, scpld, and tfpla(2)), all of which hydrolyze phosphatidylcholine (pc), trigger degranu ... | 2001 | 11380253 |
| study on indoor air pollutants: toxicity screening of suspended particulate matter. | 2001 | 11381325 | |
| nontypical bacillus cereus outbreak in a child care center. | on october 29, 1998, the garland health department in texas investigated seven illnesses at a church day school. the six children and one adult had handled hydrated, orange-colored rice before consuming a meal. b. cereus organisms were found in the rice at an estimated concentration of 5.6 x 10(5) per gram. as far as the authors can determine, this outbreak is the first one documented to have occurred from indirect ingestion of b. cereus by way of contaminated hands, as opposed to ingestion of t ... | 2001 | 11381470 |
| discrimination between bacterial spore types using time-of-flight mass spectrometry and matrix-free infrared laser desorption and ionization. | we demonstrate that molecular ions with mass-to-charge ratios (m/z) ranging from a few hundred to 19 050 can be desorbed from whole bacterial spores using infrared laser desorption and no chemical matrix. we have measured the mass of these ions using time-of-flight mass spectrometry and we observe that different ions are desorbed from spores of bacillus cereus, bacillus thuringiensis, bacillus subtilis, and bacillus niger. our results raise the possibility of identifying microorganisms using mas ... | 2001 | 11393860 |
| an enzymatic assay for quantifying sphingomyelin in tissues and plasma from humans and mice with niemann-pick disease. | sphingomyelin is an important lipid component of cell membranes and lipoproteins which can be hydrolyzed by sphingomyelinases into ceramide and phosphorylcholine. the type a and b forms of niemann-pick disease (npd) are lipid storage disorders due to the deficient activity of the enzyme acid sphingomyelinase, and the resultant accumulation of sphingomyelin in cells and tissues. in this paper we report a new, enzyme-based method to quantify the levels of sphingomyelin in tissues and plasma of nor ... | 2001 | 11399033 |
| oligopeptide permease is required for expression of the bacillus thuringiensis plcr regulon and for virulence. | plcr is a pleiotropic regulator of virulence factors in the insect pathogen bacillus thuringiensis and in the opportunistic human pathogen bacillus cereus. it activates the transcription of at least 15 genes encoding extracellular proteins, including phospholipases c, proteases and enterotoxins. expression of the plcr gene is autoregulated and activated at the onset of stationary phase. here, we used mini-tn10 transposition to generate a library of b. thuringiensis mutants, with the goal of char ... | 2001 | 11401703 |
| meningoencephalitis caused by bacillus cereus in a neonate. | we report on a newborn boy, who was delivered at 26 weeks' gestation by emergency caesarean section because of a prolapsed cord and breech presentation. grade iv hyaline membrane disease subsequently developed, for which a surfactant was given. on day 8, there were frequent apnoeic attacks, and on day 30, marked irritability developed, as did intermittent stiffening of all four limbs. the anterior fontanelle was bulging and tense, and the cerebrospinal fluid was found to be turbid. gram staining ... | 2001 | 11406681 |
| reaction of b. cereus bacteria and peroxidase enzymes under pressures >400 mpa. | it is known that b. cereus (gram-positive bacteria) and peroxidase enzymes are resistant to pressures of approximately 400 mpa in fruit and vegetable products among others. the aim of the present work is to have knowledge about their behavior when using pressures >400 mpa without other combined treatments. the results showed that b. cereus was not inactivated at pressures of 1000 mpa for 15 min at 20 degrees c. in peroxidase enzymes the results remained similar and the pressure of 1000 mpa for 3 ... | 2001 | 11409973 |
| sensitive fluorescent quantitation of myo-inositol 1,2-cyclic phosphate and myo-inositol 1-phosphate by high-performance thin-layer chromatography. | a non-radioactive micro-assay for the cyclic phosphodiesterase reaction catalyzed by bacillus cereus phosphatidylinositol-specific phospholipase c is described. the assay involves high-performance thin-layer chromatography on silica gel to resolve the substrate (myo-inositol 1,2-cyclic phosphate) and the product (myo-inositol 1-phosphate), followed by detection with a lead tetraacetate-fluorescein stain. the quantitation of these inositol phosphates in sample spots relative to a series of standa ... | 2001 | 11417877 |
| class ii broad-spectrum mercury resistance transposons in gram-positive bacteria from natural environments. | we have studied the mechanisms of the horizontal dissemination of a broad-spectrum mercury resistance determinant among bacillus and related species. this mer determinant was first described in bacillus cereus rc607 from boston harbor, usa, and was then found in various bacillus and related species in japan, russia and england. we have shown that the mer determinant can either be located at the chromosome, or on a plasmid in the bacillus species, and is carried by class ii mercury resistance tra ... | 2001 | 11446519 |
| kinetic study of two novel enantiomeric tricyclic beta-lactams which efficiently inactivate class c beta-lactamases. | a detailed kinetic study of the interaction between two ethylidene derivatives of tricyclic carbapenems, lek 156 and lek 157, and representative beta-lactamases and d-alanyl-d-alanine peptidases (dd-peptidases) is presented. both compounds are very efficient inactivators of the enterobacter cloacae 908r beta-lactamase, which is usually resistant to inhibition. preliminary experiments indicate that various extended-spectrum class c beta-lactamases (act-1, cmy-1, and mir-1) are also inactivated. w ... | 2001 | 11451677 |
| minor cytotoxic and antibacterial compounds from the rhizomes of amomum aculeatum. | a new cytotoxic 1,7-dioxa-dispiro[5.1.5.2]pentadeca-9,12-dien-11-one derivative, aculeatin d, and a new alkenone, 5-hydroxy-hexacos-1-en-3-one, have been isolated as minor compounds from the rhizomes of amomum aculeatum. their structures have been determined mainly by nmr spectroscopy and mass spectrometry. aculeatin d showed high cytotoxicity against the kb and the l-6 cell line with ic(50) of 0.38 microg/ml and 1 microg/ml, respectively. additionally, it revealed remarkable activity against tw ... | 2001 | 11454360 |
| influence of food matrix on inactivation of bacillus cereus by combinations of nisin, pulsed electric field treatment, and carvacrol. | carvacrol was used as a third preservative factor to enhance further the synergy between nisin and pulsed electric field (pef) treatment against vegetative cells of bacillus cereus. when applied simultaneously with nisin (0.04 microg/ml), carvacrol (0.5 mm) enhanced the synergy found between nisin and pef treatment (16.7 kv/cm, 30 pulses) in potassium-n-2-hydroxyethylpiperazine-n-ethanesulfonic acid (hepes) buffer. the influence of food ingredients on bactericidal activity was tested using skimm ... | 2001 | 11456185 |
| molecular dynamics simulations of the mononuclear zinc-beta-lactamase from bacillus cereus. | herein, we report molecular dynamics simulations of the mononuclear form of the bacillus cereuszinc-beta-lactamase. we studied two different configurations which differ in the presence of a zinc-bound hydroxide or a zinc-bound water and in the protonation state of the essential his210 residue. contacts of the catalytically important residues (asp90, his210, cys168, etc.) with the zinc center are characterized by the md analyses. the nature of the zn-oh(2) --> his210 proton transfer pathway conne ... | 2001 | 11457108 |
| new antioxidant and antimicrobial ellagic acid derivatives from pteleopsis hylodendron. | bioassay-guided isolation of two new compounds, 3,4-methylenedioxy-3'-o-methyl-4'-o-glucoside ellagic acid (1) and the pteleoellagic acid derivative (2), from the stem bark of pteleopsis hylodendron is reported along with 3,4-methylenedioxy-3'-o-methyl ellagic acid (3), 3,3'-di-o-methyl ellagic acid (4) and 3,3',4'-tri-o-methyl ellagic acid (5), which were obtained for the first time from this plant. the structures of these compounds were elucidated with the help of spectroscopic studies. compou ... | 2001 | 11458450 |
| contamination of pasteurized milk by bacillus cereus in the filling machine. | the contamination of pasteurized milk by bacillus cereus during the filling process was studied in two dairy plants. samples of pasteurized milk were taken at four different sites along the production line. the samples were stored at 7 degrees c for 7 d, or at 10 degrees c for 5 d, before plate counting and random selection of b. cereus isolates. isolates of b. cereus were typed by the polymerase chain reaction (pcr)-based method randomly amplified polymorphic dna (rapd). samples taken at three ... | 2001 | 11504383 |
| bacillus cereus brain abscesses occurring in a severely neutropenic patient: successful treatment with antimicrobial agents, granulocyte colony-stimulating factor and surgical drainage. | multiple brain and liver abscesses developed immediately after bacillus cereus bacteremia in a neutropenic patient with acute lymphoblastic leukemia. after even 8 weeks of antimicrobial chemotherapy together with administration of granulocyte colony-stimulating factor, every infectious process disappeared but the patient's headache has still persisted. because the wall of one brain abscess became thin and was in danger of rupturing into the ventricle, surgical drainage was performed, resulting i ... | 2001 | 11506311 |
| isolation, screening and identification of bacterial strains for degradation of predigested distillery wastewater. | three bacterial isolates from the activated sludge of a distillery wastewater treatment plant identified as xanthomonas fragariae, bacillus megaterium and bacillus cereus were found to remove cod and colour from anaerobically digested distillery wastewater in the range of 55 to 68% and 38 to 58% respectively. | 2001 | 11510138 |
| calorimetric analysis of cephalosporins using an immobilized tem-1 beta-lactamase on ni2+ chelating sepharose fast flow. | two beta-lactamases, penicillinase type i from bacillus cereus and tem-1 beta-lactamase from haemophilus ducreyi, were immobilized on a chelating sepharose fast flow column loaded with ni2+ in an active form. flow-injection analysis of beta-lactams was performed by using an enzyme column reactor fitted into the enzyme thermistor. with both enzymes it was possible to monitor both penicillins and cephalosporins. moreover, michaelis constants of the tem-1 beta-lactamase were markedly increased upon ... | 2001 | 11520032 |
| bces1, a new addition to the type iii restriction and modification family. | the nucleotide sequence of an 11-kb chromosomal bglii fragment from bacillus cereus american type culture collection (atcc) 10987 strain revealed two closely adjacent open reading frames organized in an operon, of which the deduced amino acids showed identity to the type iii restriction and modification (r/m) subunits described in gram-negative bacteria. an enhanced transcription level was revealed when the culture was grown in the presence of foreign dna. a cell-free extract from this culture r ... | 2001 | 11520613 |
| polymerase chain reaction assay for the detection of bacillus cereus group cells. | recent investigations have shown that members of the bacillus cereus group carry genes which have the potential to cause gastrointestinal and somatic diseases. although most cases of diseases caused by the b. cereus group bacteria are relatively mild, it is desirable to be able to detect members of the b. cereus group in food and in the environment. using 16s rdna as target, a pcr assay for the detection of b. cereus group cells has been developed. primers specific for the 16s rdna of the b. cer ... | 2001 | 11520616 |
| putative virulence factor expression by clinical and food isolates of bacillus spp. after growth in reconstituted infant milk formulae. | forty-seven strains representing 14 different bacillus species isolated from clinical and food samples were grown in reconstituted infant milk formulae (imf) and subsequently assessed for adherence to, invasion of, and cytotoxicity toward hep-2 and caco-2 cells. cell-free supernatant fluids from 38 strains (81%) were shown to be cytotoxic, 43 strains (91%) adhered to the test cell lines, and 23 strains (49%) demonstrated various levels of invasion. of the 21 bacillus cereus strains examined, 5 ( ... | 2001 | 11525980 |
| antibacterial activity of traditional australian medicinal plants. | fifty-six ethanolic extracts of various parts of 39 plants used in traditional australian aboriginal medicine were investigated for their antibacterial activities against four gram-positive (bacillus cereus, enterococcus faecalis, staphylococcus aureus and streptococcus pyogenes) and four gram-negative (escherichia coli, klebsiella pneumoniae, pseudomonas aeruginosa and salmonella typhimurium) bacterial species. in a plate-hole diffusion assay, 12 extracts inhibited the growth of one or more of ... | 2001 | 11535358 |
| anthrax. | bacillus anthracis was shown to be the etiological agent of anthrax by r. koch and l. pasteur at the end of the nineteenth century. the concepts on which medical microbiology are based arose from their work on this bacterium. the link between plasmids and major virulence factors of b. anthracis was not discovered until the 1980s. the three toxin components are organized in two a-b type toxins, and the bacilli are covered by an antiphagocytic polyglutamic capsule. structure-function analysis of t ... | 2001 | 11544370 |
| nontypical bacillus cereus outbreak. | 2001 | 11544846 | |
| combined effect of nisin and carvacrol at different ph and temperature levels on the viability of different strains of bacillus cereus. | the influence of ph and temperature on the bactericidal action of nisin and carvacrol on vegetative cells of different bacillus cereus strains was studied. the five strains tested showed significant differences in sensitivity towards nisin, at ph 7.0 and 30 degrees c. carvacrol concentrations of 0.3 mmol l(-1) had no effect on viability of b. cereus cells. when the same carvacrol concentration was combined with nisin, however, it resulted in a greater loss of viability of cells than when nisin w ... | 2001 | 11545214 |
| evaluation of microbiological quality of medicinal plants used in natural infusions. | consumption of preparations of medicinal plants has been increasing during the last decades in occidental societies. however, there are no effective sanitary controls of these products. to evaluate the nature and content of microbiological contamination, 62 samples of seven medicinal plants (chamomile, leaves of orange tree, flowers of linden, corn silk, marine alga, pennyroyal mint and garden sage) were studied, using conventional microbiological methods. practically all samples (96.8%) were co ... | 2001 | 11545215 |
| metal ion binding and coordination geometry for wild type and mutants of metallo-beta -lactamase from bacillus cereus 569/h/9 (bcii): a combined thermodynamic, kinetic, and spectroscopic approach. | one high affinity (nm) and one low affinity (microm) macroscopic dissociation constant for the binding of metal ions were found for the wild-type metallo-beta-lactamase from bacillus cereus as well as six single-site mutants in which all ligands in the two metal binding sites were altered. surprisingly, the mutations did not cause a specific alteration of the affinity of metal ions for the sole modified binding site as determined by extended x-ray absorption fine structure (exafs) and perturbed ... | 2001 | 11551939 |
| detection of anthrax spores from the air by real-time pcr. | to detect and isolate bacillus anthracis from the air by a simple and rapid procedure. | 2001 | 11555211 |
| a simple and sensitive detection system for bacillus anthracis in meat and tissue. | to detect and isolate bacillus anthracis from meat and tissue by rapid and simple procedures. | 2001 | 11556906 |
| a simple modified membrane filtration medium for the enumeration of aerobic spore-bearing bacilli in water. | aerobic spore-bearing bacilli have been proposed as a surrogate indicator for the removal of cryptosporidium by drinking water treatment processes. pasteurisation of samples followed by culture on non-selective media is the method of choice. using white membranes for filtration of water samples makes colony counting difficult. vital dyes such as neutral red or trypan blue can help when added to the growth medium but these dyes tend to colour the membrane and reduce the contrast between the colon ... | 2001 | 11561641 |
| thiomandelic acid, a broad spectrum inhibitor of zinc beta-lactamases: kinetic and spectroscopic studies. | resistance to beta-lactam antibiotics mediated by metallo-beta-lactamases is an increasingly worrying clinical problem. candidate inhibitors include mercaptocarboxylic acids, and we report studies of a simple such compound, thiomandelic acid. a series of 35 analogues were synthesized and examined as metallo-beta-lactamase inhibitors. the k(i) values (bacillus cereus enzyme) are 0.09 microm for r-thiomandelic acid and 1.28 microm for the s-isomer. structure-activity relationships show that the th ... | 2001 | 11564740 |
| the cell envelope-bound metalloprotease (camelysin) from bacillus cereus is a possible pathogenic factor. | a novel membrane proteinase of the nosocomial important bacteria species bacillus cereus (synonyms: camelysin, ccmp) was purified up to homogeneity as was shown by mass spectrometry in its amphiphilic form. camelysin is a neutral metalloprotease with a molecular mass of 19 kda. its unique n-terminus phe-phe-ser-asp-lys-glu-val-ser-asn-asn-thr-phe-ala-ala-gly-thr-leu-asp-leu-thr-leu-asn-pro-lys-thr-leu-val-asp-(ile-lys-asp)- was not detected in the protein data bases during blast searches, but in ... | 2001 | 11566257 |
| gern, an endospore germination protein of bacillus cereus, is an na(+)/h(+)-k(+) antiporter. | gern, a bacillus cereus spore germination protein, exhibits homology to a widely distributed group of putative cation transporters or channel proteins. gern complemented the na(+)-sensitive phenotype of an escherichia coli mutant that is deficient in na(+)/h(+) antiport activity (strain knabc). gern also reduced the concentration of k(+) required to support growth of an e. coli mutant deficient in k(+) uptake (strain tk2420). in a fluorescence-based assay of everted e. coli knabc membrane vesicl ... | 2001 | 11566988 |
| fluorescent amplified fragment length polymorphism analysis of norwegian bacillus cereus and bacillus thuringiensis soil isolates. | we examined 154 norwegian b. cereus and b. thuringiensis soil isolates (collected from five different locations), 8 b. cereus and 2 b. thuringiensis reference strains, and 2 bacillus anthracis strains by using fluorescent amplified fragment length polymorphism (aflp). we employed a novel fragment identification approach based on a hierarchical agglomerative clustering routine that identifies fragments in an automated fashion. no method is free of error, and we identified the major sources so tha ... | 2001 | 11571195 |
| isolation of bacillus thuringiensis from intertidal brackish sediments in mangroves. | intertidal brackish sediments in mangroves were examined for isolation of bacillus thuringiensis strains with novel toxicity spectra. a total of 18 b. thuringiensis isolates were recovered from eight sediment samples (36.4%) out of 22 samples tested. the frequency of b. thuringiensis was 1.3% among the colonies of bacillus cereus/b. thuringiensis group. while five isolates were allocated to the four h serogroups, the majority of the isolates were serologically untypable or untestable. two isolat ... | 2001 | 11572461 |
| novel immuno-fret assay method for bacillus spores and escherichia coli o157:h7. | novel immunofluorescence resonance energy transfer (immuno-fret) assays for both bacillus cereus spores and escherichia coli o157:h7 are reported. both assays involve the use of dual (qsy-7 and oregon green 514-antibody)-labeled spores or vegetative bacteria, such that oregon green 514-labeled antibodies are quenched by proximal qsy-7 molecules that are covalently bound to the dual (oregon green 514 and qsy-7)-labeled cells. upon introduction of unlabeled bacteria or spores, in the respective as ... | 2001 | 11573946 |
| challenge testing of the lactoperoxidase system in pasteurized milk. | to determine the role of lactoperoxidase (lp) in inhibiting the growth of micro-organisms in pasteurised milk. | 2001 | 11576311 |
| effects of amino acids on the amidation of polyaromatic carboxylic acids by bacillus cereus. | the soil bacterium bacillus cereus tim-r01 efficiently transformed polyaromatic carboxylic acids (paca) such as 4-biphenylcarboxylic acid (4-bpca), 4-biphenylacetic acid, and 4-phenoxybenzoic acid into their corresponding amides. the amidation activity was expressed at 37 degrees c (ph 7-8) in the presence of grown cells in nutrients under an aerobic atmosphere. other strains of b. cereus, ifo 3001 and iam 1229, also gave the amide from 4-bpca. in phosphate-buffered saline (pbs), the addition of ... | 2001 | 11577715 |
| inexplicable suppression of hepatic uptake of gallium-67, a case report. | we describe here a case report of a patient with acute lymphocytic leukemia in whom hepatic gallium-67 (ga-67) uptake was suppressed. the patient was hospitalized with increasing dyspnea. in ga-67 scintigraphy, there was no hepatic uptake, although other physiological uptake was clearly observed. in addition, the scintigraphy showed increased accumulation in the right lung consistent with infection. we considered possible reasons for these findings. the patient had no history of chemotherapy or ... | 2001 | 11577765 |
| distribution of s-layers on the surface of bacillus cereus strains: phylogenetic origin and ecological pressure. | bacillus anthracis, bacillus cereus and bacillus thuringiensis have been described as members of the bacillus cereus group but are, in fact, one species. b. anthracis is a mammal pathogen, b. thuringiensis an entomopathogen and b. cereus a ubiquitous soil bacterium and an occasional human pathogen. in two clinical isolates of b. cereus, in some b. thuringiensis strains and in b. anthracis, an s-layer has been described. we investigated how the s-layer is distributed in b. cereus, and whether phy ... | 2001 | 11578310 |
| bacillus cereus keratitis associated with contact lens wear. | we report the first case of contact lens-related bacillus cereus keratitis and ulcer associated with b. cereus contamination of the contact lens case. this is also the first study to investigate and establish the genetic identity of an organism isolated from the cornea and contact lens case in a patient with contact lens-associated keratitis. | 2001 | 11581057 |
| a catalytic diad involved in substrate-assisted catalysis: nmr study of hydrogen bonding and dynamics at the active site of phosphatidylinositol-specific phospholipase c. | phosphatidylinositol-specific phospholipase cs (pi-plcs, ec 3.1.4.10) are ubiquitous enzymes that cleave phosphatidylinositol or phosphorylated derivatives, generating second messengers in eukaryotic cells. a catalytic diad at the active site of bacillus cereus pi-plc composed of aspartate-274 and histidine-32 was postulated from the crystal structure to form a catalytic triad with the 2-oh group of the substrate [heinz, d. w., et al. (1995) embo j. 14, 3855-3863]. this catalytic diad has been o ... | 2001 | 11583175 |
| molecular dynamics simulations of the mononuclear zinc-beta-lactamase from bacillus cereus complexed with benzylpenicillin and a quantum chemical study of the reaction mechanism. | herein, we present results from md simulations of the michaelis complex formed between the b. cereus zinc-beta-lactamase enzyme and benzylpenicillin. the structural and dynamical effects induced by substrate-binding, the specific role of the conserved residues, and the near attack conformers of the michaelis complex are discussed. quantum chemical methods (hf/6-31g* and b3lyp/6-31g*) are also applied to study the hydrolysis reaction of n-methylazetidinone catalyzed by a monozinc system consistin ... | 2001 | 11583551 |
| molecular characterization of a phosphatidylcholine-hydrolyzing phospholipase c. | while searching for a phospholipase c (plc) specific for phosphatidylcholine in mammalian tissues, we came across such an activity originating from a contamination of pseudomonas fluorescens. this psychrophilic bacterium was found to contaminate placental extracts upon processing in the cold. the secreted phosphatidylcholine-hydrolyzing plc was purified by a combination of chromatographic procedures. as substrates, the enzyme preferred dipalmitoyl-phosphatidylcholine and 1-palmitoyl-2-arachidono ... | 2001 | 11589699 |
| mode of action of beta-barrel pore-forming toxins of the staphylococcal alpha-hemolysin family. | staphylococcal alpha-hemolysin is the prototype of a family of bacterial exotoxins with membrane-damaging function, which share sequence and structure homology. these toxins are secreted in a soluble form which finally converts into a transmembrane pore by assembling an oligomeric beta-barrel, with hydrophobic residues facing the lipids and hydrophilic residues facing the lumen of the channel. besides alpha-hemolysin the family includes other single chain toxins forming homo-oligomers, e.g. beta ... | 2001 | 11595629 |
| effect of bacillus cereus exocellular factors on human intestinal epithelial cells. | to gain insight on the biological effects of the exocellular factors produced by bacillus cereus, culture filtrate supernatants of different strains were coincubated with differentiated caco-2 cells. exocellular factors were able to detach enterocyte-like cells from the substratum after 1 h of incubation. in addition, microvilli effacing and dramatic changes on the cellular surface of enterocytes were found after incubation periods as short as 20 min. since cell detachment was not inhibited by f ... | 2001 | 11601702 |
| inhibitory activity of honey against foodborne pathogens as influenced by the presence of hydrogen peroxide and level of antioxidant power. | antimicrobial activity of honey has been attributed to hydrogen peroxide, which is produced by naturally occurring glucose oxidase, and phenolic compounds, although lethality of and inhibition by these and other components against microorganisms vary greatly, depending on the floral source of nectar. this study was undertaken to compare honeys from six floral sources for their inhibitory activity against escherichia coli o157:h7, salmonella typhimurium, shigella sonnei, listeria monocytogenes, s ... | 2001 | 11603859 |
| growth and toxin profiles of bacillus cereus isolated from different food sources. | eleven strains of bacillus cereus isolated from milk and meat products have been used to study growth and sporulation profiles in detail. polymerase chain reaction (pcr) using primers detecting cold shock protein a gene signatures (cspa), showed that none of the strains were the newly suggested species in the b. cereus group, b. weihenstephanensis, comprising psychrotolerant cereus strains, although one of the strains grew at 4 degrees c, two at 6 degrees c and seven grew at 7 degrees c. one of ... | 2001 | 11603861 |
| dynamics of mononuclear cadmium beta-lactamase revealed by the combination of nmr and pac spectroscopy. | the two metal sites in cadmium substituted beta-lactamase from bacillus cereus 569/h/9 have been studied by nmr spectroscopy ((1)h, (15)n, and (113)cd) and pac spectroscopy ((111m)cd). distinct nmr signals from the backbone amides are identified for the apoenzyme and the mononuclear and binuclear cadmium enzymes. for the binuclear cadmium enzyme, two (113)cd nmr signals (142 and 262 ppm) and two (111m)cd pac nuclear quadrupole interactions are observed. two nuclear quadrupole interactions are al ... | 2001 | 11603983 |
| presensitization of microorganisms by acid treatments to low dose gamma irradiation with special reference to bacillus cereus. | effect of acid presensitization to low dose gamma irradiation on bacillus cereus in sheep/goat meat stored at refrigeration temperature was assessed. food grade organic acids (viz. propionic, lactic and acetic acids) were used as presensitizers followed by treatment with 1, 2 and 3 kgy irradiation doses (co(60)). two percent acetic acid plus 3 kgy irradiation elicited most effective to lower the total viable count and b. cereus count. combination of treatments availed to reduce the dose required ... | 2001 | 22062253 |
| production of bacteriocin-like metabolites by lactic acid cultures isolated from sucuk samples. | a total of 51 sucuk samples, obtained from different regions of turkey, were examined for lactic acid bacteria (lab) strains producing putative bacteriocin-like metabolites. for detection of antagonistic activity, agar spot and well diffusion assay tests were used. lactobacillus sake lb790, listeria monocytogenes li6, staphylococcus aureus st44 and escherichia coli nrrl b-3704 were used as indicator organisms. strains having antimicrobial activity were also tested against clostridium perfringens ... | 2000 | 22060908 |
| regression analyses for evaluating the influence of bacillus cereus on alfalfa yield under variable disease intensity. | abstract we developed and tested regression methods to exploit the variability in disease inherent in field experiments, and applied the methods to evaluate strains of bacillus cereus for biocontrol efficacy. four b. cereus strains were tested for their effect on alfalfa (medicago sativa) performance in 16 field trials planted during 1993 to 1996 at multiple sites in wisconsin. to evaluate performance of the strains, we used the ratio of (metalaxyl response)/(untreated control response) as a mea ... | 2000 | 18944546 |
| antimicrobial effects of propolis extracts on escherichia coli and staphylococcus aureus strains resistant to various antibiotics and some microorganisms. | abstract propolis is collected by worker honey bees from the trees. the bees pack propolis on their hind legs, carry it to their colony, and use it as a sterilant in the hive. propolis has also been used by humans since early times for various purposes and, especially, as a medicine because of its antimicrobial properties. in this study, antimicrobial effects of propolis samples on three escherichia coli and two staphylococcus aureus strains that are multiresistant to antibiotics and on salmonel ... | 2000 | 19236174 |
| antimicrobial potency of the leaf - stalk extract of curcuma longa (linn). | the methanolic extract of the leaf-stalk of curcuma longa linn, was tested for its minimum inhibitor concentration (mic) against gram positive-staphylococcus aureus, bacillus pumilus, bacillus subtilis, klebsiella pnemoniae, bacillus cereus, streptococcus pneumoniae, lactobacillus arabinosus and gram negative e.coli, shigella dysenteriae, shigella sonnei, shigella boydii, salmonella typhimurium, proteus mirabilis, and vibrio cholerae strains, further, the ones of inhibition produced by the crude ... | 2000 | 22557005 |
| management of suspected nosocomial infection: an audit of 19 hospitalized patients with septicemia caused by bacillus species. | from april to august of 2000, bacillus spp. were detected in the blood culture of 29 patients in a hospital in japan. of these patients, 19 had clinical signs of septicemia; positive culture in the remaining 10 patients was attributed to contamination with skin flora at the site of puncture. of the 18 strains evaluated, 15 were bacillus cereus, 2 were bacillus subtilis, and one was bacillus licheniformis. the only hospital death observed was that of a patient who had no clinical signs of septice ... | 2000 | 11135704 |
| unrelated bone marrow as a source of bacillus cereus causing septic shock. | 2000 | 11149745 | |
| the effect of in ovo or day-of-hatch subcutaneous antibiotic administration on competitive exclusion culture (preempt) establishment in neonatal chickens. | the effect of in ovo or day-of-hatch subcutaneous antibiotic administration on the detection of antibiotic residues in yolk sac or blood serum samples and the potential for observed residues to interfere with competitive exclusion (ce) culture establishment was compared in three experiments. the in ovo or subcutaneous administration of gentamicin sulfate or ceftiofur sodium was associated with detectable levels of antibiotic residues in yolk sac or blood serum samples in experiment 1. further, t ... | 2000 | 11092319 |
| homoduplex and heteroduplex polymorphisms of the amplified ribosomal 16s-23s internal transcribed spacers describe genetic relationships in the "bacillus cereus group". | bacillus anthracis, bacillus cereus, bacillus mycoides, bacillus pseudomycoides, bacillus thuringiensis, and bacillus weihenstephanensis are closely related in phenotype and genotype, and their genetic relationship is still open to debate. the present work uses amplified 16s-23s internal transcribed spacers (its) to discriminate between the strains and species and to describe the genetic relationships within the "b. cereus group," advantage being taken of homoduplex-heteroduplex polymorphisms (h ... | 2000 | 11097928 |
| growth of bacteria in inoculated platelets: implications for bacteria detection and the extension of platelet storage. | recent reports from europe have advocated the use of bacterial culturing of platelets on day 2 or 3 of storage to extend the shelf life of platelets to 7 days, thereby reducing the outdating of platelets and preserving a limited medical resource. to assess the optimal timing, the necessary sensitivity, and the possible efficacy of bacterial detection, the bacterial growth characteristics were reviewed in 165 platelet units, each inoculated on the day of collection with one of the following organ ... | 2000 | 11099657 |
| cooperative, synergistic and antagonistic haemolytic interactions between haemolysin bl, phosphatidylcholine phospholipase c and sphingomyelinase from bacillus cereus. | haemolysis of erythrocytes from different species (sheep, bovine, swine and human), caused by various combinations of phosphatidylcholine (pc)-preferring phospholipase c (pc-plc), sphingomyelinase (smase) and the three-component, pore-forming toxin haemolysin bl (hbl) from bacillus cereus was analysed. the lytic potency of hbl did not correlate with phospholipid (pl) content, but lysis by the individual or combined enzymes did. smase alone lysed ruminant erythrocytes, which contain 46-53% sphing ... | 2000 | 11101661 |
| occurrence of bacillus thuringiensis in fresh waters of japan. | bacillus thuringiensis was recovered at a relatively high frequency from both running and still fresh waters in natural environments of kyushu, japan. of 107 water samples examined, 53 (49.5%) contained this organism. the frequency of b. thuringiensis colonies was 4.4% among 4414 colonies of the bacillus cereus/b. thuringiensis group. the density of this bacterium in fresh waters averaged 0.45 cfu/ml. serologically, b. thuringiensis isolates were assigned to 26 h serotypes. of these, h14/36 (h s ... | 2000 | 10688688 |
| a novel spore peptidoglycan hydrolase of bacillus cereus: biochemical characterization and nucleotide sequence of the corresponding gene, slel. | the exudate of germinated spores of b. cereus ifo 13597 in 0.15 m kcl-50 mm potassium phosphate (ph 7.0) contained a spore-lytic enzyme which has substrate specificity for fragmented spore cortex from wild-type organisms (cortical-fragment-lytic enzyme [cfle]), in addition to a previously characterized germination-specific hydrolase which acts on intact spore cortex (spore cortex-lytic enzyme [scle]) (r. moriyama, s. kudoh, s. miyata, s. nonobe, a. hattori, and s. makino, j. bacteriol. 178:5330- ... | 2000 | 10692353 |
| mapping the catalytic pocket of phospholipases a2 and c using a novel set of phosphatidylcholines. | a set of radioiodinatable phosphatidylcholines (pcs) derivatized with the bolton-hunter reagent (bhpcs) was synthesized to probe the substrate recognition and activity of phospholipases. a common feature of this series is the presence of a bulky 4-hydroxyphenyl group at the end of the fatty acyl chain attached to position sn-2. the distance between the end group and the glycerol backbone was varied by changing the length of the intervening fatty acyl chain (3-25 atoms). except for the shortest, ... | 2000 | 10698694 |
| in vitro antibacterial activity and mechanism of action of j-111,225, a novel 1beta-methylcarbapenem, against transferable imp-1 metallo-beta-lactamase producers. | imp-1 beta-lactamase, a class b zinc metallo-enzyme encoded by the transferable bla(imp) gene, is known to confer high-level resistance to carbapenems as well as to penicillins and cephalosporins. j-111, 225 is a novel 1beta-methylcarbapenem with a structurally unique side chain comprising a trans-3,5-disubstituted pyrrolidinylthio moiety at the c2 position. it inhibited 17 serratia marcescens and two pseudomonas aeruginosa imp-1-producing clinical isolates at a concentration of 32 mg/l (range 4 ... | 2000 | 10702544 |
| solution conformations of short-chain phosphatidylcholine. substrates of the phosphatidylcholine-preferring plc of bacillus cereus. | the phosphatidylcholine (pc)-preferring phospholipase c (plc) from bacillus cereus (plc(bc)) hydrolyzes various 1,2-diacyl derivatives of pc at different rates. substrates with side chains having eight or more carbons are present in micellular form in aqueous media and are processed most rapidly. the catalytic efficiency (k(cat)/k(m)) for the hydrolyses of short-chain pcs at concentrations below their respective critical micelle concentrations also decreases as the side chains become shorter, an ... | 2000 | 10704924 |
| mutations in the gerp locus of bacillus subtilis and bacillus cereus affect access of germinants to their targets in spores. | the gerp1 transposon insertion mutation of bacillus cereus is responsible for a defect in the germination response of spores to both l-alanine and inosine. the mutant is blocked at an early stage, before loss of heat resistance or release of dipicolinate, and the efficiency of colony formation on nutrient agar from spores is reduced fivefold. the protein profiles of alkaline-extracted spore coats and the spore cortex composition are unchanged in the mutant. permeabilization of gerp mutant spores ... | 2000 | 10715007 |
| bacillus cereus fatal bacteremia and apparent association with nosocomial transmission in an intensive care unit. | bacillus cereus has sometimes been implicated in food poisoning and in opportunistic infections of seriously ill patients. this report describes an unusual case of persistent bacteremia and multiple organ failure associated with b. cereus in a patient admitted to our institution for lung cancer. the patient was undergoing treatment with an antimicrobial agent (imipenem) that was shown to be effective against the micro-organism in vitro. no portal of entry for the strain was detected. after treat ... | 2000 | 10716089 |
| characterization of vim-2, a carbapenem-hydrolyzing metallo-beta-lactamase and its plasmid- and integron-borne gene from a pseudomonas aeruginosa clinical isolate in france. | pseudomonas aeruginosa col-1 was identified in a blood culture of a 39-year-old-woman treated with imipenem in marseilles, france, in 1996. this strain was resistant to beta-lactams, including ureidopenicillins, ticarcillin-clavulanic acid, cefepime, ceftazidime, imipenem, and meropenem, but remained susceptible to the monobactam aztreonam. the carbapenem-hydrolyzing beta-lactamase gene of p. aeruginosa col-1 was cloned, sequenced, and expressed in escherichia coli dh10b. the deduced 266-amino-a ... | 2000 | 10722487 |
| the choline binding site of phospholipase c (bacillus cereus): insights into substrate specificity. | the phosphatidylcholine-preferring phospholipase c from bacillus cereus (plc(bc)) is a 28.5 kda enzyme with three zinc ions in its active site. the roles that a number of amino acid residues play as zinc ligands and in binding and catalysis have been elucidated. recent mechanistic studies indicate that the rate of the reaction is limited by a proton-transfer step during chemical hydrolysis and not substrate binding or product release. an x-ray structure of plc(bc) complexed with a phosphonate in ... | 2000 | 10727235 |
| gene cloning, nucleotide sequencing, and purification and characterization of the d-stereospecific amino-acid amidase from ochrobactrum anthropi sv3. | the gene encoding the d-stereospecific amino-acid amidase from ochrobactrum anthropi sv3 was cloned and sequenced. analysis of 7.3 kb of genomic dna revealed the presence of six orfs, one of which (daaa) encodes the d-amino-acid amidase. this enzyme, daaa, is composed of 363 amino-acid residues (molecular mass 40 082 da), and the deduced amino-acid sequence exhibits homology to alkaline d-peptidase from bacillus cereus df4-b (32% identity), dd-peptidase from streptomyces r61 (29% identity), and ... | 2000 | 10727942 |
| phosphatidylcholine-specific phospholipase c-mediated induction of phospholipase d activity in fas-expressing murine cells. | we have previously reported that fas cross-linking resulted in the activation of phosphatidylcholine-specific phospholipase c (pc-plc) and the subsequent activation of protein kinase c (pkc) and phospholipase d (pld) in a20 cells. in an attempt to correlate the existence of pc-plc activity and activation of pld by fas activation among various fas-expressing murine cell lines, we have investigated the effect of anti-fas monoclonal antibody on pc-plc and pld activities in a20, p388d1 and yac-1 cel ... | 2000 | 11007187 |
| research on factors allowing a risk assessment of spore-forming pathogenic bacteria in cooked chilled foods containing vegetables: a fair collaborative project. | vegetables are frequent ingredients of cooked chilled foods and are frequently contaminated with spore-forming bacteria (sfb). therefore, risk assessment studies have been carried out, including the following: hazard identification and characterisation--from an extensive literature review and expertise of the participants, b. cereus and c. botulinum were identified as the main hazards; exposure assessment--consisting of determination of the prevalence of hazardous sfb in cooked chilled foods con ... | 2000 | 11016602 |
| new developments in chromogenic and fluorogenic culture media. | this review describes some recent developments in chromogenic and fluorogenic culture media in microbiological diagnostic. the detection of beta-d-glucuronidase (gud) activity for enumeration of escherichia coli is well known. e. coli o157:h7 strains are usually gud-negative and do not ferment sorbitol. these characteristics are used in selective media for these organisms and new chromogenic media are available. some of the new chromogenic media make the salmonella diagnostic easier and faster. ... | 2000 | 11016610 |
| properties of bacillus cereus and other bacilli contaminating biomaterial-based industrial processes. | this paper is an overview on bacilli in industrial processes, with focus on food grade paper and paperboard production. paperboards mainly contain sporeforming bacteria belonging to the genera bacillus, paenibacillus and brevibacillus, usually found in quantities from < 50 to 250 cfu g(-1) homogenized paperboard. of those frequently found, bacillus cereus group, b. licheniformis, b. subtilis and brevibacillus brevis are important for food hygiene because of their hydrolytic activities on food co ... | 2000 | 11016612 |
| structural model requirements to describe microbial inactivation during a mild heat treatment. | the classical concept of d and z values, established for sterilisation processes, is unable to deal with the typical non-loglinear behaviour of survivor curves occurring during the mild heat treatment of sous vide or cook-chill food products. structural model requirements are formulated, eliminating immediately some candidate model types. promising modelling approaches are thoroughly analysed and, if applicable, adapted to the specific needs: two models developed by casolari (1988), the inactiva ... | 2000 | 11020040 |