Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| malignant pleural effusions and their treatment by intercostal talc pleurodesis. | an analysis is presented of 44 patients with malignant pleural effusion treated by intercostal talc pleurodesis, all except eight of them between 1966 and 1971. | 2004 | 534605 |
| the surgical treatment of atypical rectovaginal fistulas. | 2004 | 525686 | |
| [effect of certain factors on a decrease in the ribonucleic acid content in saccharomyces carlsbergensis brewer's yeasts via autofermentation]. | 2004 | 525457 | |
| measurement of cause-specific death rate from civil registration in korea: 1970--1974. | 2004 | 524920 | |
| [univentricular heart and its clinical picture]. | 2004 | 523212 | |
| vaginal epithelial changes associated with in utero des exposure. | to determine the true prevalence of vaginal epithelial changes in des-exposed offspring, the national cancer institute funded the desad project, a study designed to follow up these women for 4 years and to determine the prevalence of cancer and various other abnormalities in des-exposed women. 3339 women have constituted the project study cohort as of 12/31/76. each woman in the desad project undergoes breast, pelvic and colposcopic examinations, as well as vaginal and cervical cytology. details ... | 2004 | 523034 |
| [measurements of sputum viscoelasticity using conventional dynamic measurements and the newly developed raised cosine pulse method (author's transl,]. | 2004 | 522300 | |
| a bird's eye view of occupational medicine. | this article defines occupational medicine, outlines the training involved, and describes various phases of occupational medicine. it is based on the author's experience and training. | 2004 | 522191 |
| genetics of acheiropodia ("the handless and footless families of brazil"): xi. pathologic aspects. | we report another case of acheiropodia, a rare autosomal recessive trait of distal limb deficiency, to date seen only in brazil in persons of portuguese ancestry. the patient's parent were first cousins; she had two younger affected sisters. she presented with signs of vascular insufficiency of the lower stumps, which after amputation were found to lack large vessels below the knee. | 2004 | 517582 |
| [hepato-cortical syndrome]. | 2004 | 516620 | |
| influence of increased anatomical dead space no functional residual capacity of lungs and the lung clearance index. | a study of the influence of added anatomical dead space on the functional residual capacity of the lung was carried out on a total number of 40 healthy subjects of ages ranging between 21 and 50 years. the functional residual capacity was found to decrease as additional dead space was introduced in the breathing circuit. this decrease was associated with an increase in tidal volume, lung functions, as given by the lung clearance index, were found to deteriorate with each addition of dead space. ... | 2004 | 521123 |
| interpositional microvascular vein grafting. | the application of interpositional microvascular vein grafting in replantation surgery, a detailed account of the technique developed and utilised in the microvascular research and training laboratory, and clinical experience with this technique are described. vein grafting is of particular value to eliminate tension in anastomoses, bridge vascular defects, and has made feasible the salvage of severe crushing and avulsion amputation in the hand. | 2004 | 520879 |
| individual and familial patterns in four dimensions of lateral preference. | 2004 | 514490 | |
| [relation of acute cardiovascular diseases to meteorological and synoptic parameters in the climatic conditions in kiev]. | 2004 | 516631 | |
| [evaluation of the effectiveness of intra arterial infusion of drugs in thrombo-obliterative diseases of the arteries of the limbs]. | 2004 | 1133910 | |
| differential impact of right and left hemisphere lesions on facial emotion and object imagery. | thirty-six patients with unilateral hemispheric lesions of the right hemisphere (rhd), left hemisphere (lhd), or no neurologic disease were evaluated on two tasks of visual imagery: one involved imagery for facial emotions and the other involved imagery for common objects. as a group, the rhd patients were more impaired on the emotional than the object imagery task, whereas the lhd patients showed the opposite pattern. individual case analyses suggested that the rhd group consisted of different ... | 2004 | 1782533 |
| [overnutrition]. | 2004 | 492388 | |
| [ultrastructure of the liver in patients with cholesterol stones receiving chenodesoxycholic acid (author's transl)]. | the ultrastructure of the liver was investigated in 5 normal persons and 10 patients with cholesterol stones. in the patients numerous intramitochondrial crystals could be found; precipitated cholesterol-digitonin complexes were found as well in the canaliculi, the sinus and the pericanalicular areas. 5 patients received chenodesoxycholic acid (10 mg/kg/day); liver biopsies taken 3 and 6 months after start of treatment did not show any more the changes described above. | 2004 | 491815 |
| double graduation in new south wales. | 2004 | 470686 | |
| draft recommendations on basic medical education: general medical council, united kingdom. | 2004 | 470642 | |
| [colonic diverticulosis-diverticulitis. what is their origin? how can they be prevented?]. | 2004 | 470586 | |
| latent cirrhosis in singapore. a morphological and aetiological study. | 2004 | 451637 | |
| newborn bleeding disorders: a practical approach. | 2004 | 471551 | |
| [an analysis of the side effects and complications in 1300 angiographies performed using seldinger's technique (author's transl)]. | 2004 | 481064 | |
| abortion and the nhs: the first decade. | 2004 | 427432 | |
| plasmapheresis and severe glomerulonephritis. | 2004 | 427395 | |
| the aetiology and outcome of abruptio placentae. | a series of 193 cases of abruptio placentae in a hospital population of 35,217 is described. this is an incidence of 0.55%. in the series both age and parity, but not pre-eclampsia or anaemia, are significant associated factors. the recurrence rate of abruptio placentae was 5.6%. there were no maternal deaths and the perinatal mortality was 35%. epidural analgesia does not abolish the pain of the abruptio placentae in spite of abolishing the pain of labour. | 2004 | 419953 |
| the fine structure of subcortical neurofibrillary tangles in progressive supranuclear palsy. | the fine structure of subcortical neurofibrillary tangles was investigated in pallidum, substantia nigra, periaqueductal gray, pontine reticular gray, and dentate nucleus of 5 autopsy cases of progressive supranuclear palsy. only tangles due to straight 150 a wide filaments have been detected. these findings, obtained from a large series of cases and areas examined, confirm previous observations on the fine structure of neurofibrillary tangles in progressive supranuclear palsy and suggest that t ... | 2004 | 419938 |
| quantitative study of the structure-stability relationship of tcvo(iii) complexes. | this paper describes the quantitative structure-stability relationship of tcvon2s2 compounds using a stability indicator based on the solid angle factor sum (sas) for predicting the in vitro stability. the sas values of six tcvon2s2 and one tcvon4 (d,l-hmpao) complexes were calculated from their x-ray crystallography data. the rank order of in vitro stability of these compounds, as measured by ligand exchange reaction, is directly related to that of the sas values. the sas values are potentially ... | 2004 | 2172189 |
| [symptom changes and rational therapy of vitamin b12 absorption disturbances (author's transl)]. | longterm parenteral therapy with the physiological depot form of vitamin b12 (aquocobalamin) is now the treatment of choice for pernicious anemia and funicular myelopathy. an optimal dosage scheme is given. high-dose oral and intrathecal applications of viatamin b12 are also possible in the individual case. if symptoms of neurological deficiency are already present in funicular myelopathy, additional physiotherapeutic measures, supervision of bladder function, prophylaxis of decubitus ulcers and ... | 2004 | 409935 |
| ada honors dr. lynch, bestows 7 honorary memberships. | 2004 | 387851 | |
| effects of neomycin alone and in combination with cholestyramine on serum cholesterol and fecal steroids in hypercholesterolemic subjects. | effects of neomycin were studied on serum cholesterol and fecal steroids in hypercholesterolemic patients during a short treatment period (4 wk) and a long treatment period (16 mo), using small (1.5 g/d) and large (up to 6 g/d) doses alone and in combination with cholestyramine. in the short-term low-dose study the decrease in serum cholesterol by 21% was associated with a proportionate increase in fecal cholesterol elimination as neutral sterols through impaired cholesterol absorption. serum ch ... | 2004 | 387820 |
| the new editor-in-chief of artery, henry f. hoff, ph.d. | 2004 | 395931 | |
| [epileptic seizures in cerebral tumors (author's transl)]. | 2004 | 412179 | |
| echocardiographic diagnosis and evaluation of cardiomyopathies: idiopathic hypertrophic subaortic stenosis, chagas' heart disease and endomyocardial fibrosis. | echocardiographic investigations on patients with hypertrophic cardiomyopathy with obstruction have been detailed and compared with the changes found in sixty patients with chronic chagas' 'cardiomyopathy'. these changes are similar to those encountered in congestive cardiomyopathy. endomyocardial fibrosis is rare in venezuela, but six patients have been found in that country and the echocardiographic changes in one of these patients has been included in this study. | 2004 | 412175 |
| the myelodysplasia and hydrocephalus program in nebraska: a 15 year review of costs and benefits. | 2004 | 412114 | |
| [dr. petr truhlár, on his 70th birthday]. | 2004 | 387562 | |
| profile: janet mcdowell, lvn. | 2004 | 356008 | |
| naproxen in the treatment of rheumatoid arthritis. | naproxen, a new nonsteroidal anti-inflammatory analgesic drug was found to be better than placebo and as effective as indomethacin in the treatment of rheumatoid arthritis. the drug was well tolerated, and appears to be a useful alternative to more established medication such as aspirin. | 2004 | 355954 |
| [development in conservatively treated scoliosis in patients with myelomeningocele (patients of the years 1964-1977)]. | the advances in urological and neurosurgical management in myelomeningocoele patients have led to an increased survival rate. the extremely complex spinal deformity presents a major treatment challenge. clinical and radiological evolution of scoliosis treated conservatively for at least 10 years was studied. eighty-nine myelomeningocoele patients born between 1964 and 1977 were reviewed. thirty-one (35%) were noted to have scoliosis (10 congenital type, 21 developmental type). all congenital for ... | 2004 | 1772015 |
| [mass-psychology and analysis of the ego--one life-time later]. | 2004 | 331406 | |
| histopathologic changes and the immune response within the jejunal mucosa in infants and children. | jejunal biopsies obtained from 45 children referred for a variety of clinical conditions were examined histologically and by standard immunofluorescence methods. the number of plasma cells was recorded per "mucosal tissue unit". the results showed that most of the immunoglobulin a (iga) counts in infants under 3 yr with normal mucosa were low and that there was a rise in the number of iga plasma cells starting after this age. despite this, under pathologic conditions (partial villous atrophy wit ... | 2004 | 384350 |
| computer diagnosis of abnormal cardiac rhythms employing a new p-wave detector for interval measurement. | 2004 | 343984 | |
| [100 years of professional expositions of heating- and air-technology installations. on the 9th ish 1977 from 23--27 march 1977]. | 2004 | 321293 | |
| pocket calculator program low cost implementation of an epidemic model. | 2004 | 535361 | |
| electroconvulsive therapy: an overview. | modifications in technique, including the use of anesthesia and muscle-relaxing drugs, make electroconvulsive therapy safe and easy to administer. there are few contraindications, complications are rare, and efficacy is well-documented. | 2004 | 441398 |
| miliary tuberculosis involving the brain: mr findings. | 2004 | 1414778 | |
| [the effect of blood-filled meshwork of arachnoid villi in connection with posttraumatic brain edema (author's transl)]. | based on the kellie-munro doctrine according to which the intracranial compartments--blood, brain, and csf--are in a state of pressure and volume equilibrium, we examined if traumatic lesions of the liquor absorption passages could change the intracranial pressure (icp). therefore, we reviewed the arachnoid villi and granulations of patients which died as a result of head injuries. in cases of traumatic subarachnoidal hemorrhages the meshwork of arachnoid villi are filled throughout with blood. ... | 2004 | 419940 |
| normothermic coronary perfusion during aortic valve replacement. | 2004 | 305765 | |
| [outpatient care in uric acid disorders]. | in 35 patients with uricopathy (arthritis urica, essential hyperuricaemia) the serum uric acid values, consumption of alcohol, overweight, hypertension and liver enzyma under dietary and medicamentous therapy (allopurinol) were investigated. here during an observation lasting 12--48 months allopurinol proved as a well tolerable uricostatic drug which was sufficiently effective also with a considerable exogenic purine application. we only insufficiently succeeded in influencing overweight and alc ... | 2004 | 539007 |
| [therapeutic endoscopy in the gastrointestinal tract]. | in selected chapters of the therapeutic endoscopy we tried to represent secure knowledge, actual tendencies and partly future prognoses. hereby completeness cannot be expected. however, it became clear that numerous physical principles became useful for the therapy with the endoscope. chemical techniques, such as the litholysis of gall-stones are only at present used in our field. with all scepticism against the own field of interest one may say that the operative therapeutic endoscopy, as it is ... | 2004 | 539002 |
| inducibility of metallothionein biosynthesis in cultured normal and menkes kinky hair disease fibroblasts: effects of copper and cadmium. | metallothionein biosynthesis is not induced by extracellular copper in menkes kinky hair disease (mkhd) or in normal cultured fibroblasts under the conditions of these experiments. in the presence of copper, mkhd fibroblasts also incorporated less cysteine than did normal fibroblasts. extracellular cadmium greatly enhanced the uptake of cysteine in both normal and mkhd cultures. by the technique of polyacrylamide gel electrophoresis, it was demonstrated that metallothionein is induced by cadmium ... | 2004 | 471576 |
| [low-molecular weight nuclear rna's]. | 2004 | 395786 | |
| non-union of carpal scaphoid fractures in children. | fracture of the carpal scaphoid is uncommon in children, but does occur and may fail to unite. eight patients with established non-union have been reiewed, with an average follow-up of almost four years. all non-unions were grafted with autogenous bone. excellent clinical and radiological results have been obtained. it is concluded that non-union in children is best managed by bone grafting through the anterior approach. possible aetiological factors concerned in non-union of scaphoid fractures ... | 2004 | 321463 |
| [methods of dosed pressure in the complex treatment of cicatricial deformities of the face following burns]. | 2004 | 524169 | |
| acute viral respiratory infections in children. | 2004 | 506792 | |
| [long-term results following breast reconstruction]. | a ten-year experience of breast reconstruction (br) at the gustave-roussy institute (paris) provides material for a long-term analysis of carcinologic and cosmetic results. carcinologic results have been studied in a group of 120 breast reconstructions which was matched to a group of 120 mastectomies without br. no statistical difference was observed both for the overall survival and the disease-free survival in both groups. the percentage of local recurrences in the reconstructed breast was inf ... | 2004 | 2666356 |
| history of the use and toxicity of thorotrast. | 2004 | 387405 | |
| prevalence and antibiotic resistant shigellae among primary school children in urban calabar, nigeria. | in a recent study, involving the examination of 593 stool samples obtained from randomly selected children from three primary schools in calabar, shigella spp. were isolated from 123 (20.7%) of the samples. the infection rate was highest among the younger children with (64.2%) 79/123 of the isolates coming from children under 10 years of age. infection rate was comparable among boys and girls (p = 0.1). only two species of shigella were isolated viz: shigella flexneri 12.0% (71/593) and shigella ... | 2004 | 18839866 |
| isatin-derived antibacterial and antifungal compounds and their transition metal complexes. | a series of isatins incorporating thiazole, thiadiazole, benzothiazole and p-toluene sulfonyl hydrazide moieties, along with their cobalt(ii), copper(ii), nickel(ii) and zinc(ii) metal complexes have been synthesized and characterized by elemental analyses, molar conductances, magnetic moments, ir, nmr and electronic spectral data. these compounds have been screened for antibacterial activity against escherichia coli, bacillus subtillis, shigella flexneri, staphylococcus aureus, pseudomonas aeru ... | 2004 | 15648656 |
| shigella flexneri strains produce bacteriocins active against members of the human microbial intestinal flora. | the principal aim of this work was to detect the bacteriocinogenic capacity of s. flexneri strains on members of the human intestinal flora. a total of 49 bacteriocinogenic s. flexneri strains were isolated from individuals of both sexes and different ages. the bacteriocins were detected by means of the drop method using e. coli and b. fragilis as target strains. the serotypes of the s. flexneri were determined. the producer capacity of bacteriocins was analysed in 10 different colonies of the s ... | 2004 | 17061528 |
| antimicrobial resistance of shigella flexneri serotypes in israel during a period of three years: 2000-2002. | this is a surveillance study of the antimicrobial resistance of the s. flexneri group in the context of its serotype diversity. it includes 1422 isolates, which were sent to the national shigella reference centre (nsrc) by hospitals and outpatient clinics in israel during a 3-year period (2000-2002). the strains were identified and classified according to the prevalence and antigenic structure of their serotypes. all samples were checked for resistance to ampicillin (amp), trimethoprim-sulphamet ... | 2004 | 15635961 |
| [choice of informative characteristics for the characterization of the adaptive process in the population of rod-shaped bacteria]. | different data characterizing the growth and development of rod-shaped bacterial cells (shigella flexneri strains s and rd) at the initial stage of the formation of their populations on a solid culture medium were analyzed with a view to the evaluation of the adaptive process. the study revealed that lo and lk (initial and end of bacterial lenght) and tau (generation time) of cells were the optimal informative characteristics. the determination of these characteristics on the cell population lev ... | 2004 | 15636132 |
| [construction of sw480 cell model identifying shigella virulent genes]. | signature-tagged mutagenesis (stm) is a novel technology with high throughput screening ability to identify virulent genes of pathogen in vivo. an appropriate animal or cell line model is one of prerequisites by exploiting this technique. in order to apply stm to shigella flexneri, rc426 was constructed as an attenuated mutant with chloramphenicol resistance and aroa and virg genes inactivated by homologous recombination; another attenuated strain t32 was used as an oral s. flexneri 2a vaccine d ... | 2004 | 15640048 |
| screening and identification of shigella flexneri 2a virulence-related genes induced after invasion of epithelial cells. | an in vivo expression technology (ivet) was applied to screen s. flexneri 2a genes induced after invasion of epithelial cells, and virulence-related genes were further identified by mutational analysis. thirteen intracellular induced genes were identified with a hela cell infection model. of these, two were identified as alkylation-related genes; one was related to metabolism; one encoded a transcriptional regulator; three were identified as insertion elements; three appeared to be antisense to ... | 2004 | 15620105 |
| multiple roles of clan (caspase-associated recruitment domain, leucine-rich repeat, and naip ciia het-e, and tp1-containing protein) in the mammalian innate immune response. | naip ciia het-e and tp1 (nacht) family proteins are involved in sensing intracellular pathogens or pathogen-derived molecules, triggering host defense responses resulting in caspase-mediated processing of proinflammatory cytokines and nf-kappab activation. caspase-associated recruitment domain, leucine-rich repeat, and nacht-containing protein (clan), also known as ice protease-activating factor, belongs to a branch of the nacht family that contains proteins carrying caspase-associated recruitme ... | 2004 | 15528373 |
| identification of escherichia coli o157 : h7 genes influencing colonization of the bovine gastrointestinal tract using signature-tagged mutagenesis. | enterohaemorrhagic escherichia coli (ehec) cause acute gastroenteritis in humans that may be complicated by life-threatening systemic sequelae. the predominant ehec serotype affecting humans in the uk and north america is o157 : h7 and infections are frequently associated with contact with ruminant faeces. strategies to reduce the carriage of ehec in ruminants are expected to lower the incidence of human ehec infections; however, the molecular mechanisms underlying persistence of ehec in ruminan ... | 2004 | 15528651 |
| bacteria as dna vaccine carriers for genetic immunization. | genetic immunization with plasmid dna vaccines has proven to be a promising tool in conferring protective immunity in various experimental animal models of infectious diseases or tumors. recent research focuses on the use of bacteria, in particular enteroinvasive species, as effective carriers for dna vaccines. attenuated strains of shigella flexneri, salmonella spp., yersinia enterocolitica or listeria monocytogenes have shown to be attractive candidates to target dna vaccines to immunological ... | 2004 | 15532991 |
| cloning, sequencing and partial characterisation of sorbitol transporter (srlt) gene encoding phosphotransferase system, glucitol/sorbitol-specific iibc components of erwinia herbicola atcc 21998. | a dna fragment of approximately 1500 bp, harbouring the sorbitol transport gene (srlt), was amplified from the chromosomal dna of erwinia herbicola atcc 21998 by pcr and cloned in escherichia coli jm109. degenerate oligonucleotide primers used were designed based on the conserved regions in the gene sequences within the gut operon of e. coli (gene bank accession no. j02708) and the srl operon of erwinia amylovora (gene bank accession no. y14603). the cloned dna fragment was sequenced and found t ... | 2004 | 15560368 |
| escape of intracellular shigella from autophagy. | the degradation of undesirable cellular components or organelles, including invading microbes, by autophagy is crucial for cell survival. here, shigella, an invasive bacteria, was found to be able to escape autophagy by secreting icsb by means of the type iii secretion system. mutant bacteria lacking icsb were trapped by autophagy during multiplication within the host cells. icsb did not directly inhibit autophagy. rather, shigella virg, a protein required for intracellular actin-based motility, ... | 2004 | 15576571 |
| experimental study on the antibacterial effect of origanum volatile oil on dysentery bacilli in vivo and in vitro. | to observe the germistatic and germicidal effects of origanum volatile oil (ovi) on the dysentery bacteria, the abdominal cavity of mice was infected with shigella sonne (sh. sonnei) and shigella flexneri (sh. flexneri) f2a. after ovi was given to the mice via gastric lavage, the effects of ovi on the infected mice were observed. the minimal inhibitory concentration (mic) and minimal bactericidal concentration (mbc) for dysentery bacteria were determined in vitro. the results showed that origanu ... | 2004 | 15587409 |
| trends in population-based active surveillance for shigellosis and demographic variability in foodnet sites, 1996-1999. | shigella is a common cause of diarrhea in the united states, and accurate surveillance is needed to determine the burden of illness that they cause. active surveillance for culture-confirmed shigella infection was done as part of the foodborne diseases active surveillance network (foodnet). a total of 4317 cases of shigellosis were reported during 1996-1999 in the original foodnet surveillance areas. the average annual incidence was 7.4 cases/100,000 population. the incidence was similar during ... | 2004 | 15095187 |
| intranasal immunization with multivalent group a streptococcal vaccines protects mice against intranasal challenge infections. | we have previously shown that a hexavalent group a streptococcal m protein-based vaccine evoked bactericidal antibodies after intramuscular injection. in the present study, we show that the hexavalent vaccine formulated with several different mucosal adjuvants and delivered intranasally induced serum and salivary antibodies that protected mice from intranasal challenge infections with virulent group a streptococci. the hexavalent vaccine was formulated with liposomes with or without monophosphor ... | 2004 | 15102757 |
| stereotypic and specific elements of the human colonic response to entamoeba histolytica and shigella flexneri. | the clinical presentations of bacillary dysentery caused by shigella, and amoebic dysentery caused by the protozoan parasite entamoeba histolytica, can be indistinguishable, with both organisms causing colonic mucosal damage and ulceration. however, the two organisms are quite distinct, and have very different pathogenic mechanisms. this raises the fundamental question of whether the similar clinical manifestations reflect a stereotypic response of the human gut to mucosal injury, or whether the ... | 2004 | 15104595 |
| comparative analysis of transcriptional regulatory elements of glutamate-dependent acid-resistance systems of shigella flexneri and escherichia coli o157:h7. | the ability to withstand an acid-challenge of ph 2.5 or less by shigella flexneri is a necessary trait for virulence and is generally believed to be restricted to the stationary-phase of growth. earlier reports indicated the glutamate-dependent acid-resistance (gdar) system of s. flexneri is under the regulation of rpos, the gene encoding alternative sigma factor that is induced in the stationary-growth phase. the present study reports that unlike escherichia coli o157:h7, s. flexneri cells when ... | 2004 | 15109732 |
| in vitro adhesion and invasion inhibition of shigella dysenteriae, shigella flexneri and shigella sonnei clinical strains by human milk proteins. | shigella is the etiological agent of shigellosis, a disease responsible for more than 500,000 deaths of children per year, in developing countries. these pathogens colonize the intestinal colon, invade, spreading to the other enterocytes. breastfeeding plays a very important role in protecting infants from intestinal infections. amongst milk compounds, glycosylated proteins prevent the adhesion of many enteropathogens in vitro. the aim of this work was to determine the effect of human milk prote ... | 2004 | 15115555 |
| microbial-gut interactions in health and disease. epithelial cell responses. | pathogenic bacteria use many strategies to secure their survival within the host. enteropathogens exploit intestinal epithelial cells in many ways, including the manipulation of normal cellular functioning, or of cellular structural components, or by the induction of signalling pathways, such as the production of pro-inflammatory cytokines. however, the enterocyte warns the host of impending danger and, in turn, elicits a protective response. pathogens are detected by epithelial cells owing to t ... | 2004 | 15123076 |
| crystal structure of the oxidized form of the periplasmic mercury-binding protein merp from ralstonia metallidurans ch34. | in ralstonia metallidurans ch34, the gene merp encodes for a periplasmic mercury-binding protein which is capable of binding one mercury atom. the metal-binding site of merp consists of the highly conserved sequence gmtcxxc found in the family that includes metallochaperones and metal-transporting atpases. we purified merp from r.metallidurans ch34 and solved its crystal structure under the oxidized form at 2.0a resolution. superposition with structures of other metal-binding proteins shows that ... | 2004 | 15123428 |
| branching sites and morphological abnormalities behave as ectopic poles in shape-defective escherichia coli. | certain mutants in escherichia coli lacking multiple penicillin-binding proteins (pbps) produce misshapen cells containing kinks, bends and branches. these deformed regions exhibit two structural characteristics of normal cell poles: the peptidoglycan is inert to dilution by new synthesis or turnover, and a similarly stable patch of outer membrane caps the sites. to test the premise that these aberrant sites represent biochemically functional but misplaced cell poles, we assessed the intracellul ... | 2004 | 15130123 |
| detection of shigella by a pcr assay targeting the ipah gene suggests increased prevalence of shigellosis in nha trang, vietnam. | shigella spp. are exquisitely fastidious gram-negative organisms which frequently escape detection by traditional culture methods. to get a more complete understanding of the disease burden caused by shigella in nha trang, vietnam, real-time pcr was used to detect shigella dna. randomly selected rectal swab specimens from 60 shigella culture-positive patients and 500 shigella culture-negative patients detected by population-based surveillance of patients seeking care for diarrhea were processed ... | 2004 | 15131166 |
| the chromosome of shigella flexneri bacteriophage sf6: complete nucleotide sequence, genetic mosaicism, and dna packaging. | shigella flexneri temperate bacteriophage sf6 is of interest in part because its prophage expresses the oac gene that alters the antigenic properties of the surface o-antigen polysaccharide of its host bacterium. we have determined the complete sequence of its 39,044 bp genome. the sequence shows that sf6 is a member of the canonical lambdoid phage group, and like other phages of this type has a highly mosaic genome. it has chromosomal regions that encode proteins >80% identical with at least 15 ... | 2004 | 15136040 |
| preparation of synthetic glycoconjugates as potential vaccines against shigella flexneri serotype 2a disease. | the synthesis of three neoglycopeptides incorporating carbohydrate haptens, differing in length, covalently linked to a non natural universal t helper peptide is disclosed. they were synthesized according to a blockwise strategy based on the condensation of appropriate di-, tri-, and tetrasaccharide trichloroacetimidate donors onto an azidoethyl 2-acetamido-2-deoxybeta-d-glucopyranoside acceptor. use of thiol-maleimide coupling chemistry allowed site-selective efficient conjugation. | 2004 | 15136809 |
| sensitization of gram-negative and gram-positive bacteria to jenseniin g by sublethal injury. | jenseniin g, a bacteriocin produced by propionibacterium thoenii p126, is active against related propionibacteria and some lactic acid bacteria and is sporostatic to botulinal spores. the objective of this study was to evaluate the effects of sublethal stress on jenseniin g activity. bacillus cereus, enterococcus faecalis, escherichia coli, listeria monocytogenes, salmonella typhimurium, shigella flexneri, staphylococcus aureus, and yersinia enterocolitica were subjected to temperature, acid, an ... | 2004 | 15151241 |
| chromosomal and plasmid-encoded enzymes are required for assembly of the r3-type core oligosaccharide in the lipopolysaccharide of escherichia coli o157:h7. | the type r3 core oligosaccharide predominates in the lipopolysaccharides from enterohemorrhagic escherichia coli isolates including o157:h7. the r3 core biosynthesis (waa) genetic locus contains two genes, waad and waaj, that are predicted to encode glycosyltransferases involved in completion of the outer core. through determination of the structures of the lipopolysaccharide core in precise mutants and biochemical analyses of enzyme activities, waaj was shown to be a udp-glucose:(galactosyl) li ... | 2004 | 15155763 |
| laboratory-confirmed shigellosis in the united states, 1989-2002: epidemiologic trends and patterns. | during 1989-2002, a total of 208,368 laboratory-confirmed shigella infections were reported to the centers for disease control and prevention. shigella sonnei accounted for 71.7%, shigella flexneri accounted for 18.4%, shigella boydii accounted for 1.6%, and shigella dysenteriae accounted for 0.7% of infections; for 7.6%, no serogroup was reported. national incidence rates ranged from 7.6 cases per 100,000 persons in 1993 to 3.7 cases per 100,000 persons in 1999. incidence rates for s. boydii, s ... | 2004 | 15156473 |
| antibacterial and antifugal mono- and di-substituted symmetrical and unsymmetrical triazine-derived schiff-bases and their transition metal complexes. | a new series of antibacterial and antifungal triazine-derived mono- and di-substituted (symmetrical and unsymmetrical) schiff-bases and their cobalt(ii), copper(ii), nickel(ii) and zinc(ii) metal complexes have been synthesized and characterized by their elemental analyses, molar conductances, magnetic moments and ir and electronic spectral measurements. ir spectra indicated the ligands to act as tridentate towards divalent metal ions via a trazine-n, the azomethine-n and, indole-nh and deproton ... | 2004 | 15449731 |
| invasive characteristics of apathogenic shigella flexneri 5a2c mutant obtained under the effect of furazolidone. | apathogenic shigella flexneri 5a2c mutant treated with furazolidone can infect eucaryotic cells. these bacteria contain no virulence genes responsible for sh. flexneri invasion, which seems to be the cause of their apathogenicity. the capacity of bacteria to penetrate into eucaryotic cells correlates with the appearance of ecp 32 protease specifically cleaving actin. | 2004 | 15455123 |
| psi-phi: exploring the outer limits of bacterial pseudogenes. | because bacterial chromosomes are tightly packed with genes and were traditionally viewed as being optimized for size and replication speed, it was not surprising that the early annotations of sequenced bacterial genomes reported few, if any, pseudogenes. but because pseudogenes are generally recognized by comparisons with their functional counterparts, as more genome sequences accumulated, many bacterial pathogens were found to harbor large numbers of truncated, inactivated, and degraded genes. ... | 2004 | 15479949 |
| mutants of agrobacterium tumefaciens virg gene that activate transcription of vir promoter in escherichia coli. | the vira and virg two-component regulatory system is essential for transcriptional activation of virulence (vir) genes in agrobacterium tumefaciens in the presence of inducer molecules. the vira/virg mediated vir gene transcription depends on a specific interaction between the c-terminal domain of the alpha subunit (rpoa) of a. tumefaciens rna polymerase (rnap) and n-terminal domain of the virg. however, such interaction does not occur between rnap of e. coli and the virg, thus vir gene activati ... | 2004 | 15486707 |
| [identification of a protein interacting with shigella flexneri ipac invasin by yeast two-hybrid system]. | two-hybrid system was applied to screen proteins interacting with ipac in the host cell. by using two-hybrid system, the bait plasmid containing ipac gene was constructed and designated pgbkt-ipac. a human hela cdna library was screened to isolate protein factors that might interact with ipac. among the 2 x 10(6) clones screened, 22 positive clones were picked out. sequence analysis revealed that two of them contained cdna fragments from collagenase. subsequently the domain of ipac interacting w ... | 2004 | 15487505 |
| osa protein constitutes a strong oncogenic suppression system that can block vir-dependent transfer of incq plasmids between agrobacterium cells and the establishment of incq plasmids in plant cells. | the osa (oncogenic suppressive activity) gene of the incw group plasmid psa is sufficient to suppress tumorigenesis by agrobacterium tumefaciens. osa confers oncogenic suppression by inhibiting vire2 protein export. this result is similar, but not identical, to that of oncogenic suppression by the incq plasmid rsf1010. we conducted a series of experiments to compare oncogenic suppression by these two systems. agrobacterium strains harboring plasmids containing osa are more able to effect oncogen ... | 2004 | 15489437 |
| shiga toxin-encoding bacteriophages--genomes in motion. | shiga toxins (stx) represent a group of bacterial toxins that are involved in human and animal disease. stx are mainly produced by escherichia coli isolated from human and non-human sources, shigella dysenteriae type 1, and sporadically, by citrobacter freundii, enterobacter cloacae and shigella flexneri. the genes encoding stx are encoded in the genome of heterogeneous lambdoid prophages (stx-converting bacteriophages; stx-phages). they are located in a similar position in the late region of th ... | 2004 | 15493821 |
| deletion in the shigella enterotoxin genes further attenuates shigella flexneri 2a bearing guanine auxotrophy in a phase 1 trial of cvd 1204 and cvd 1208. | we created a live, attenuated, oral shigella vaccine by constructing a lineage of guanine auxotrophs and conducted a double-blind, placebo-controlled trial to ascertain (1) the attenuation profile of delta guaba shigella flexneri 2a, which harbors deletions in the guanine nucleotide synthesis pathway (cvd 1204); (2) additional attenuation conferred by deletions in set and sen genes encoding shigella enterotoxins (shets) 1 and 2, respectively (cvd 1208); and (3) the relative immunogenicity of the ... | 2004 | 15499528 |
| complex multiple antibiotic and mercury resistance region derived from the r-det of nr1 (r100). | the sequence of the 45.2-kb multidrug and mercury resistance region of prmh760, a large plasmid from a clinical isolate of klebsiella pneumoniae collected in 1997 in australia, was completed. most of the modules found in the resistance determinant (r-det), or tn2670, region of nr1 (also known as r100), isolated from a shigella flexneri strain in japan in the late 1950s, were present in prmh760 but in a different configuration. the location was also different, with the tn2670-derived region flank ... | 2004 | 15504849 |
| traditional medicine in sakarya province (turkey) and antimicrobial activities of selected species. | traditional medicine in north-west of turkey (sakarya province) were studied during a 2 months field study by interviewing local informants from several villages. plant species used to treat infections were tested for antimicrobial activity. information was collected for 46 plant species from 30 families and for 5 animal species. twenty four of the plant species were cultivated. most used families were asteraceae, cucurbitaceae, lamiaceae and rosaceae and the most used plants were artemisia absi ... | 2004 | 15507351 |
| single-molecule approach to bacterial genomic comparisons via optical mapping. | modern comparative genomics has been established, in part, by the sequencing and annotation of a broad range of microbial species. to gain further insights, new sequencing efforts are now dealing with the variety of strains or isolates that gives a species definition and range; however, this number vastly outstrips our ability to sequence them. given the availability of a large number of microbial species, new whole genome approaches must be developed to fully leverage this information at the le ... | 2004 | 15516592 |
| direct and indirect transcriptional activation of virulence genes by an arac-like protein, pera from enteropathogenic escherichia coli. | the plasmid-encoded per regulatory locus of enteropathogenic escherichia coli (epec) is generally considered to consist of three genes, pera, perb and perc. pera, a member of the arac-like family of transcriptional regulators, is known to be an activator of its own promoter (autoactivation) as well as of the plasmid-located bfp operon encoding bundle-forming pili, but its role in activation of the chromosomal locus of enterocyte effacement (lee) pathogenicity island, which confers the property o ... | 2004 | 15522091 |
| blockwise approach to fragments of the o-specific polysaccharide of shigella flexneri serotype 2a: convergent synthesis of a decasaccharide representative of a dimer of the branched repeating unit. | the d'a'b'(e')c'dab(e)c decasaccharide representative of a dimer of a frame-shifted pentasaccharide repeating unit of the o-specific polysaccharide of shigella flexneri 2a was synthesized as its methyl glycoside by condensing a pentasaccharide donor (d'a'b'(e')c') and a pentasaccharide acceptor (dab(e)c-ome). several convergent routes to these two building blocks, involving either the ab linkage or the bc linkage as the disconnection site, were evaluated in comparison to the linear strategy. the ... | 2004 | 14961653 |
| identification and functional characterization of the lactococcus lactis rfb operon, required for dtdp-rhamnose biosynthesis. | dtdp-rhamnose is an important precursor of cell wall polysaccharides and rhamnose-containing exopolysaccharides (eps) in lactococcus lactis. we cloned the rfbacbd operon from l. lactis mg1363, which comprises four genes involved in dtdp-rhamnose biosynthesis. when expressed in escherichia coli, the lactococcal rfbacbd genes could sustain heterologous production of the shigella flexneri o antigen, providing evidence of their functionality. overproduction of the rfbac proteins in l. lactis resulte ... | 2004 | 14973085 |
| shigella flexneri infection: pathogenesis and vaccine development. | shigella flexneri is a gram-negative bacterium which causes the most communicable of bacterial dysenteries, shigellosis. shigellosis causes 1.1 million deaths and over 164 million cases each year, with the majority of cases occurring in the children of developing nations. the pathogenesis of s. flexneri is based on the bacteria's ability to invade and replicate within the colonic epithelium, which results in severe inflammation and epithelial destruction. the molecular mechanisms used by s. flex ... | 2004 | 14975529 |