Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| anal cancer in chinese: human papillomavirus infection and altered expression of p53. | aim:to detect the presence of hpv dna and study the alteration of p53 expression in anal cancers in chinese.methods:hpv dna was amplified by pcr.the amplified hpv dna was classified by dbh. hpv antigen and p53 expression were respectively detected by immunohistochemistry.results:hpv dna was amplified only in one case of squamous cell carcinoma of the 72 chinese anal cancers and further classified as hpv type 16. others were all hpv negative. hpv antigen and p53 expression were also detected in t ... | 1998 | 11819303 |
| [a study on the relationship between hpv infection and the oncogenesis of primary squamous carcinoma of the lung.]. | to research the relationship between human papillomavirus (hpv) infection and the oncogenesis of primary squamous carcinoma of the lung. | 1998 | 20863461 |
| correlation between human papillomavirus infection and clinicopathological parameters in anal canal carcinoma. | we evaluated human papillomavirus (hpv) dna in 17 anal canal tumors and its correlation with symptomatology, tumor extension and prognosis. five squamous carcinomas and 4 cloacogenic tumors resulted hpv+. statistical analysis showed no correlation between hpv infection and tumor morphology, lymph node involvement or prognosis, and no significant difference in the duration of symptoms between hpv+ and hpv- patients. hpv are involved in the pathogenesis of the tumors, but are not responsible for a ... | 1997 | 21590048 |
| a fast and simple in situ pcr method to detect human papilloma virus infection in archival paraffin-embedded tissues. | we describe a simple and fast method for the detection and localization of low copy numbers of hpv dna in formalin-fixed and paraffin-embedded archival tissues. we have developed a protocol for direct bl situ-pcr in order to demonstrate its convenience in rapid and reproducible assessment of hpv infection in unknown biopsies. the morphological aspect of the tissues has been maintained, despite the multiple steps of fixation, permeabilization and thermal cycling, and positivity has been detected ... | 1997 | 21528242 |
| human papillomavirus infection and anal neoplasia. | recent clinical advances in hiv and aids treatment are allowing people to live longer and enjoy a higher quality of life. however, many people may now have to confront other diseases, especially malignancies, that may not have developed in the course of a short but fatal hiv infection. the mechanisms of cancer development in people with hiv are complex and not completely understood. there are three factors that are known to influence the development of malignancies: the absence of a protective i ... | 1997 | 11364694 |
| examining the complex relationship of human papillomavirus to cervical dysplasia and carcinoma. | human papillomavirus (hpv) infection is quite common in women and is clearly the major risk factor for cervical intraepithelial neoplasia and invasive cervical cancer. hpv dna has been detected in 80% to 90% of cin 3 lesions and invasive cervical cancers. while the most common presentation is warts, or condylomata, many infections are detected only by pap smear cytologic evidence. we still do not have a clear understanding of hpv latency, reactivation, subclinical infection without apparent dise ... | 1997 | 9746693 |
| effect of retinoic acid on hpv titration and colposcopic changes in korean patients with dysplasia of the uterine cervix. | retinoids, a family of molecules capable of profound impact on many biological functions, have antiproliferative, differentiative, and immunomodulatory properties. the present study assessed the effect of 13-cis-retinoic acid (13-cra) treatment in 13 chronic cervicitis and 52 cervical intraepithelial neoplasia patients. we examined low- and high-risk human papilloma virus titer (using the hybrid capture method) and made a colposcopic and cervicographic examination before and after treatment with ... | 1997 | 9589358 |
| human papillomavirus and epstein-barr virus in epithelial carcinomas of the head and neck region. | human papillomavirus (hpv) dna has been detected in carcinomas of the upper aerodigestive tract. however, studies of the subject show considerable variation in their results, and the causal relationship between hpv and squamous cell carcinomas of the head and neck area still remains to be determined. epstein-barr virus (ebv) is consistently detected in nasopharyngeal carcinoma lesions, but little is known about its association with other carcinomas of the head and neck region. the present study ... | 1997 | 9494545 |
| failure to detect human papilloma virus in cutaneous molluscum contagiosum lesions. | in a recently published study, the coexistence of hpv and mcv was demonstrated for the first time in the same molluscum contagiosum lesions from a single patient. such a finding raises many questions in the understanding of both hpv and mcv. with this finding comes the potential of new treatments against dually infected mc lesions, which normally demonstrate high resistance to therapy. furthermore, the finding of hpv and mcv coexistence raises questions on the effects of mcv immunosuppressive ge ... | 1997 | 9494606 |
| [cancer of the anal canal]. | anal carcinoma is a rare malignant tumor, it occurs in only 0.02% of all malignant neoplasms. in mexico, the incidence is of 1.5%, and only 0.18% belong to the anal canal. in recent years it has been reported an increased incidence of this tumor due to the association with the human papilloma virus in hiv positive patients. the most common histological forms are the epidermoid and the cloacogenic carcinomas. the most relevant prognostic factors are the size of the tumor and the presence of lymph ... | 1997 | 9480525 |
| detection of hpv-16 dna by pcr in histologically cancer free lymph nodes from patients with cervical cancer. | the prognostic value of detection of human papillomavirus (hpv) type 16 dna in histologically cancer free lymph nodes was assessed in left obturator lymph nodes from cervical cancer patients with hpv-16 positive primary tumours. hpv-16 dna was detected by polymerase chain reaction in 12 of 35 patients with histologically cancer free lymph nodes. of these 12 patients, only one developed a recurrence, suggesting hpv-16 dna detection in cancer free lymph nodes has no prognostic value. | 1997 | 9462250 |
| summary of avoidable cancers in the nordic countries. | an overview is given of the most important known causes of cancer in the five nordic countries and the resulting number of cancers that are potentially avoidable. the main causes include active and passive smoking, alcohol consumption, exposure to asbestos and other occupational carcinogens, solar and ionizing radiation, obesity, human papillomavirus infection in the female genital tract and infection with helicobacter pylori. the organs most commonly affected are those of the respiratory system ... | 1997 | 9462826 |
| the carboxyl-terminal zinc-binding domain of the human papillomavirus e7 protein can be functionally replaced by the homologous sequences of the e6 protein. | the carboxyl-terminus is necessary for the functional and structural integrity of the human papillomavirus (hpv) e7 oncoprotein. since many mutations in this domain of e7 result in the formation of unstable proteins, we have evaluated the importance of this region by replacing it with structurally related domains derived from hpv e6 proteins. biological analysis of these mutant chimeric e7/e6 proteins showed that they retained e7-specific biological activities including cooperation with the ras ... | 1997 | 9453149 |
| cancer-associated human papillomavirus types are selectively increased in the cervix of women in the first trimester of pregnancy. | 1997 | 9279838 | |
| [detection of the human papillomavirus in hyperplastic and cancerous prostatic tissue with pcr]. | human papillomavirus (hpv) infections are frequently associated with benign, pre-malignant and malignant lesions of the female genital region and male anogenital tract. recently several studies have been performed to evaluate the presence of hpv in the adenomatous and carcinomatous prostatic tissue for the epidemiological implications due to the sexual transmission, and for the possible etiologic role in the development of prostatic carcinoma, first cause of death from neoplasia in men over 60 y ... | 1997 | 9281080 |
| the human papillomavirus e7 oncoprotein can uncouple cellular differentiation and proliferation in human keratinocytes by abrogating p21cip1-mediated inhibition of cdk2. | the high risk human papillomaviruses (hpvs) are associated etiologically with the majority of human cervical carcinomas. these hpvs encode two viral oncoproteins, e6 and e7, which are expressed consistently in cervical cancers. the function of these viral oncoproteins during a productive infection is to ensure viral replication in cells that have normally withdrawn from the cell division cycle and are committed to terminal differentiation. expression of the e7 oncoprotein has been shown to lead ... | 1997 | 9284049 |
| assessment of cytotoxic t-lymphocyte phenotype using the specific markers granzyme b and tia-1 in cervical neoplastic lesions. | cervical carcinomas are closely associated with high-risk human papillomavirus (hpv) types and are preceded by cervical intraepithelial neoplasia (cin). most cin lesions regress spontaneously and will not evolve to invasive carcinoma. the cellular immune system mediated by cytotoxic t lymphocytes (ctls) and natural killer (nk) cells are thought to play an important role in the ultimate decline of cin lesions. although tia-1 is constitutively expressed in the majority of circulating t cells and d ... | 1997 | 9374383 |
| [recombinant therapeutic vaccines against cancer of the uterine cervix]. | several mechanism to evade the immune system are induced during cervical cancer development, including the decrease of expression of class i hla molecules and secretion of specific cytokines by tumoral cells. consequently, the stimulation of cytotoxic (ctl) and helper (th) t lymphocytes, as well as the natural killer (nk) cells and macrophages is very poor. the induction of immune response against tumors needs the stimulation of multiple components of the immune system: systemic stimulation of c ... | 1997 | 9381250 |
| epidemiology of human papillomavirus infections. | 1997 | 9385310 | |
| using polymerase chain reaction to human papillomavirus in oral and pharyngolaryngeal carcinomas. | increasingly, evidence has shown that human papillomavirus (hpv) plays a role in the induction of certain carcinomas. the presence of hpv sequences in 56 previously untreated oral and pharyngolaryngeal carcinomas was examined by the polymerase chain reaction (pcr). | 1997 | 9395012 |
| protective anti-tumor immunity induced by vaccination with recombinant adenoviruses encoding multiple tumor-associated cytotoxic t lymphocyte epitopes in a string-of-beads fashion. | vaccines harboring genes that encode functional oncoproteins are intrinsically hazardous, as their application may lead to introduction of these genes into normal cells and thereby to tumorigenesis. on the other hand, oncoproteins are especially attractive targets for immunotherapy of cancer, as their expression is generally required for tumor growth, making the arisal of tumor variants lacking these antigens unlikely. using murine tumor models, we investigated the efficacy of polyepitope recomb ... | 1997 | 9405669 |
| volume-activated taurine transport is differentially activated in human cervical cancer ht-3 cells but not in human papillomavirus-immortalized z183a and normal cervical epithelial cells. | 1. previous studies demonstrate that volume-sensitive chloride currents are distinctly activated in cervical cancer cells, but not in human papillomavirus (hpv)-immortalized and normal cervical cells. in the present study, the na(+)-independent volume-activated transport of taurine in three cervical cell types was investigated. 2. osmotic swelling of cervical cancer ht-3 cells suspended in na(+)-free hypotonic medium led to increased membrane uptake of taurine. this taurine uptake was effectivel ... | 1997 | 9406659 |
| seasonal fluctuations in the cervical smear detection rates for (pre)malignant changes and for infections. | the detection of diseases can exhibit seasonal fluctuations. this can be studied in cervical smears. over a 9-year observation span (january 1983-january 1992) a series of 504,093 cervical smears obtained from a routine cytology laboratory in the netherlands were examined for infections (monilia, trichomonas, actinomyces, human papilloma virus [hpv], chlamydia, and herpes) as well as for mild, moderate, and severe dysplasias, carcinoma in situ, and squamous carcinoma. statistical analysis (princ ... | 1997 | 9407207 |
| prevalence of penile human papillomavirus dna in husbands of women with and without cervical neoplasia: a study in spain and colombia. | to investigate the role of men in cervical cancer, 816 husbands of women enrolled in four case-control studies of cervical neoplasia in populations at high (colombia) and low (spain) risk for cervical cancer were interviewed. exfoliated cells from the penis were obtained and analyzed by polymerase chain reaction for the presence of human papillomavirus (hpv) dna. penile hpv dna prevalences were higher in husbands of women with cervical neoplasia than in husbands of controls. husbands of controls ... | 1997 | 9237700 |
| the role of high risk hpv lymph node positivity in the surgical staging of cancer of the uterine cervix. | the presence of oncogenic human papillomavirus (hpv) dnas in cervical carcinomas and their normal and metastatic pelvic lymph nodes is studied worldwide. does the hpv positivity without histological metastasis in the lymph nodes mean an early prognostic indicator in the progress of the disease? the authors analyzed the surgical, histological and virological data of four cervical cancer patients, whose recurrence occurred very early and died soon. after performing radical hysterectomy with precis ... | 1997 | 9408386 |
| differential regulation of the pocket domains of the retinoblastoma family proteins by the hpv16 e7 oncoprotein. | the human papillomavirus e7 oncoprotein binds to the retinoblastoma (rb) tumor suppressor protein, and the binding to rb correlates with the oncogenic potential of e7. recent studies from several laboratories indicated that the half-life of the rb protein is reduced in cells that are stably transformed with e7, suggesting that e7 could induce the proteolytic degradation of rb. to investigate whether the rb degradation is a primary effect of e7 or a result of altered cell phenotype, we sought to ... | 1997 | 9419416 |
| human papillomavirus distribution in cervical tissues of different morphology as determined by hybrid capture assay and pcr. | distribution of various types of genital human papillomavirus (hpv) in smears from histologically classified cervical lesions was determined by hybrid capture assay (hca) and was compared with a polymerase chain reaction (pcr) system using general primers (gp) in first and type specific primers (ts) in a second step. the overall agreement of high-risk hpv by hca and the more sensitive gp/ts pcr was 80.6% (204 of 253, kappa value 0.6). human papillomavirus frequency by gp/ts pcr was 14-20% higher ... | 1997 | 9421083 |
| generation of in vitro autologous human cytotoxic t-cell response to e7 and her-2/neu oncogene products using ex-vivo peptide loaded dendritic cells. | 1997 | 9286422 | |
| [detection of human papillomavirus gene sequences in laryngeal tumors and premalignant changes by polymerase chain reaction]. | human papillomavirus gene sequences have been detected in a number of malignant and benign tumours. non-oncogenic types 6 and 11 are etiological factors of benign mucosal tumours. types 16 and 18 can be detected in malignancies most often but their role in the etiopathogenesis of cancers is still unclear. in our study we examined formalin-fixed and paraffin-embedded archive laryngeal tissues containing squamous cell carcinoma, papilloma and precancerous lesions for the presence of human papillom ... | 1997 | 9289687 |
| a monoclonal antibody against intact human papillomavirus type 16 capsids blocks the serological reactivity of most human sera. | a type-specific and neutralizing mouse mab (v5) against human papillomavirus (hpv) type 16 capsids was found to block the serological reactivity of human sera with the corresponding capsids. out of 352 human serum samples tested for the presence of igg against hpv-16, more than 75% of reactive sera were completely blocked by the v5 antibody. type-specific mabs against hpv-6, -18 and -33 were also found to block serological reactivity with capsids of the corresponding hpv types for the majority o ... | 1997 | 9292008 |
| type-specific prevalence of genital human papillomaviruses in benign, premalignant, and malignant biopsies in patients from greece. | more than 30 different human papillomavirus (hpv) types infect the anogenital mucosa and are responsible for a variety of benign, premalignant, and malignant lesions including cervical cancer. the goal of this study was to determine the distribution of individual hpv types in various grads of cervical precancerous lesions and cervical carcinoma in patients from greece. | 1997 | 9293610 |
| lymphoproliferative responses to human papillomavirus (hpv) type 16 proteins e6 and e7: outcome of hpv infection and associated neoplasia. | infection with human papillomavirus (hpv) type 16 (hpv16) is a major cause of high-grade cervical intraepithelial neoplasia (cin). experiments were planned to evaluate the role of cell-mediated immunity (e.g., lymphocyte proliferation) against hpv in the natural history of hpv-associated neoplasia and to identify antigenic sequences of the hpv16 proteins e6 and e7 against which an immune response may confer protection. | 1997 | 9293919 |
| genetic alterations in patients with esophageal cancer with short- and long-term survival rates after curative esophagectomy. | the objective of this study was to ascertain the exact relation between specific oncogenes and long- and short-term survival rates in patients with esophageal cancer. | 1997 | 9296509 |
| incidence of human papillomavirus 6, 11, 16, 18 and 33 in normal oral mucosa of a greek population. | the polymerase chain reaction (pcr) was applied for the detection of human papillomavirus (hpv) infection in samples obtained from the clinically normal mucosa of the oral cavity of 169 asymptomatic subjects in northern greece. of the subjects, 9.5% were found to be infected with hpv. typing of hpv by southern blot hybridization revealed that 2.4%, 0%, 0%, 4.1%, 0.6% of the subjects were infected with hpv 16, 18, 33, 6 and 11, respectively. | 1997 | 9298359 |
| carcinomas occurring in papillomas of the nasal septum associated with human papilloma virus (hpv). | carcinomas arising in pre-existing sinonasal papillomas of the nasal septum are rare. to our knowledge only one case has been reported. we report two cases of carcinomas occurring in septal papillomas. in the first case a carcinoma developed in an exophytic papilloma 16 years after the first operation for a papilloma. in the second case a carcinoma was present at the first presentation within an inverted papilloma, and a metastasis had also developed. in the first case hpv type 6/11 was demonstr ... | 1997 | 9299655 |
| anal human papillomavirus and anal cancer. | 1997 | 9301544 | |
| [the detection of human papillomavirus in papillomas of the larynx and tonsils through immunohistochemistry and dna in situ hybridization]. | presenting a study about human papillomavirus (hpv) determination by immunohistochemistry and "in situ" hybridization, in 25 samples of squamous cell papillomata (15 tonsilar and 10 laryngeal lesions). five cases resulted positive for hpv: 2 of them for immunohistochemical probes, other 2 for "in situ" hybridization and only 1 cas showed its positivity for both techniques. all these samples belonging to laryngeal cases. no significant differences were found in antecedents, clinical and histologi ... | 1997 | 9304351 |
| analysis of hpv16, 18, 31, and 35 dna in pre-invasive and invasive lesions of the uterine cervix. | to analyse the physical state of different human papillomavirus (hpv) dnas in 55 intraepithelial and invasive hpv associated cervical neoplasms. | 1997 | 9306943 |
| prevalence of epstein-barr virus in oral squamous cell carcinomas, premalignant lesions and normal mucosa--a study using the polymerase chain reaction. | epstein-barr virus (ebv) prevalence was assessed in 12 clinically normal oral mucosas, nine premalignant lesions, 36 oral squamous cell carcinomas (osccs) and a human papillomavirus (hpv) 16 positive cell line, derived from an oscc studied. the polymerase chain reaction (pcr) with two pairs of primers with different sensitivities was used. with primers specific for the bamhiw repeat, ebv was found in 100% of osccs, in 77.8% of premalignant lesions and in 8.3% of clinically normal oral mucosas (p ... | 1997 | 9307727 |
| human papillomavirus and other risk factors for cervical cancer. | cancer of the cervix is the second most common cancer in women after cancer of the breast, and the commonest in developing countries. in the last decade the etiology of cervical cancer has been unequivocally established--over 90% of the cases in all countries are related to infections to some strains of human papillomavirus (hpv). these infections are common in young age groups and resolve spontaneously in most instances. the typical hpv deoxyribonucleic acid (dna) prevalence after the third dec ... | 1997 | 9309247 |
| is human papillomavirus an infectious cause of non-cervical anogenital tract cancers? | 1997 | 9310555 | |
| prospective seroepidemiological study of role of human papillomavirus in non-cervical anogenital cancers. | to evaluate the association between infection with the major oncogenic types of human papillomavirus and the risk of developing non-cervical anogenital cancers in a cohort followed up prospectively. | 1997 | 9310566 |
| synthesis of infectious human papillomavirus type 18 in differentiating epithelium transfected with viral dna. | the lack of a permissive system for the propagation of viral stocks containing abundant human papillomavirus (hpv) particles has hindered the study of infectivity and the early stages of hpv replication. the organotypic (raft) culture system has permitted the study of a number of the differentiation-specific aspects of hpv, including amplification of viral dna, expression of late genes, and viral morphogenesis. however, these investigations have been limited to a single virus type, namely, hpv t ... | 1997 | 9311816 |
| conserved interaction of the papillomavirus e2 transcriptional activator proteins with human and yeast tfiib proteins. | papillomavirus early gene expression is regulated by the virus gene-encoded e2 proteins. the best-characterized e2 protein, encoded by bovine papillomavirus type 1 (bpv-1), has been shown to interact with basal transcription factor iib (tfiib) and the tata binding protein basal transcription factor (n. m. rank and p. f. lambert, j. virol. 69:6323-6334, 1995). we demonstrate that the potent e2 transcriptional activator protein encoded by a gene of human pv type 16 also interacts with tfiib in vit ... | 1997 | 9311902 |
| p53 protein overexpression is common and independent of human papillomavirus infection in squamous cell carcinoma of the vulva. | human papillomavirus (hpv) infection and p53 abnormalities might both play a role in the carcinogenesis of subtypes of squamous cell carcinoma of the vulva. in this study, the authors investigated the prevalence of and the relationship between p53 overexpression and hpv infection in patients with vulvar carcinoma. | 1997 | 9317172 |
| selection of human cervical epithelial cells that possess reduced apoptotic potential to low-oxygen conditions. | since human papillomavirus (hpv) infection is strongly associated with cervical neoplasia and tumor hypoxia has prognostic significance in human cervical carcinomas, we examined the relationship between hypoxia and apoptosis in human cervical epithelial cells expressing high-risk hpv type 16 oncoproteins. in vitro, hypoxia stimulated both p53 induction and apoptosis in primary cervical epithelial cells infected with the hpv e6 and e7 genes but not in cervical fibroblasts infected with e6 and e7. ... | 1997 | 9331075 |
| skin autografts in epidermodysplasia verruciformis: human papillomavirus-associated cutaneous changes need over 20 years for malignant conversion. | epidermodysplasia verruciformis (ev) is regarded as a model for cutaneous oncogenesis associated with specific human papillomaviruses (hpvs). because genital hpv-associated carcinogenesis is a very long-lasting process requiring 20-30 years and epidemiological studies of this type for hpv-associated skin cancers are impossible in such a rare disease as ev, we observed for up to 20 years ev patients having surgery for carcinomas with consecutive autografts from uninvolved and non-sun-exposed skin ... | 1997 | 9331078 |
| immune activation in cervical neoplasia: cross-sectional association between plasma soluble interleukin 2 receptor levels and disease. | in a previous study (tsukui et al., cancer res., 56: 3967-3974, 1996), we observed an inverse association between degree of cervical neoplasia and interleukin (il) 2 production by peripheral blood mononuclear cells in response to human papillomavirus (hpv) 16 e6 and e7 peptides in vitro. this suggested that a th1-mediated cellular immune response might be important in host immunological control of hpv infection and that a lack of such a response might predispose to progression of cervical diseas ... | 1997 | 9332763 |
| serum levels of soluble tumor-necrosis-factor receptors in patients with benign and malignant hpv-associated anogenital lesions. | the levels of type-i and type-ii soluble tnf-alpha receptors (stnf-rs) were evaluated in sera from patients with various human-papillomavirus-(hpv)-associated benign and malignant anogenital lesions using specific enzyme-linked immunobiological assays. in patients with benign hpv6/11-associated condylomata acuminata, the levels of stnf-ri were significantly increased, while stnf-rii were in normal range. both types of stnf-rs were in normal range in patients with benign hpv16-associated grade-i/ ... | 1997 | 9334803 |
| common association of hpv 2 with anogenital warts in prepubertal children. | anogenital (ag) warts in 31 prepubertal children were hpv typed by nonisotopic in situ hybridization (nish) using digoxigenin-labeled probes for human papilloma virus (hpv) types 1-5, 6, 11, 16, 18, 31, and 33. mode of transmission was determined from historical, clinical, and laboratory data independent of hpv typing. hpv 2 was detected most commonly (13/31 warts) followed by hpv 6 (7/31), hpv 11 (5/31), and hpv 16 (1/31). although not reaching statistical significance, our results suggested th ... | 1997 | 9336800 |
| cervical cancer and human papillomavirus: epidemiological evidence and perspectives for prevention. | cervical cancer is a major public health problem, as it is the second most common cancer in women world-wide after breast cancer. about 80% of the half a million cases estimated to occur annually in the world, occur in developing countries. the epidemiological evidence linking human papillomavirus (hpv) to cervical cancer is reviewed. it is concluded that over 90% of cervical cancers can be attributed to certain hpv types. hpv 16 accounts for the highest proportion (50%) followed by hpv 18 (12%) ... | 1997 | 9337560 |
| in situ hybridization and its diagnostic applications in pathology. | in situ hybridization (ish) is a technique by which specific nucleotide sequences are identified in cells or tissue sections. these may be endogenous, bacterial or viral, dna or rna. on the basis of research applications, the technique is now being translated into diagnostic practice, mainly in the areas of gene expression, infection and interphase cytogenetics. diagnostic applications are most often based on short nucleotide sequences (oligomers) labelled with non-isotopic reporter molecules, a ... | 1997 | 9349226 |
| cytokeratin patterns of expression in human epithelial cell lines correlate with transcriptional activity of the human papillomavirus type 16 upstream regulatory region. | a comparison of the cat reporter activity of a plasmid which contains the 232 bp epithelial specific enhancer alone with that of plasmids which contain additional sequences from the human papillomavirus type 16 (hpv-16) upstream regulatory region (urr) revealed two markedly different patterns, in an analysis of six human epithelial cell lines. in hela, c33a and siha, the cat reporter activities of all the constructs were comparable. in contrast, in caski, hk2be6-e7 and hacat we detected very low ... | 1997 | 9349481 |
| pathogenesis of human papillomavirus infection of the genitals. | 1997 | 9350117 | |
| human papillomavirus-specific cytotoxic t lymphocytes in patients with cervical intraepithelial neoplasia grade iii. | human papillomaviruses (hpvs), especially types 16 and 18, are strongly associated with the development of cervical intraepithelial neoplasia (cin) and invasive carcinoma. the hpv e6 and e7 proteins are expressed constitutively in the majority of cin lesions and carcinomas. therefore, they are targets for the immune response against hpv and candidates for active immunotherapy. we have previously detected hpv-specific ctls from the peripheral blood mononuclear cells from a cervical cancer patient ... | 1997 | 9354449 |
| sexually transmitted infection as a cause of anal cancer. | the incidence of anal cancer has increased in recent decades, particularly among women. to identify underlying risk factors, we conducted a population-based case-control study in denmark and sweden. | 1997 | 9358129 |
| carcinoma of the lung in okinawa, japan: with special reference to squamous cell carcinoma and squamous metaplasia. | in okinawa, a subtropical island in southern japan, squamous cell carcinoma (scc), especially the well-differentiated form, is prevalent, while this form is relatively rare in both the mainland and other countries (e.g. united states of america). more patients with scc from okinawa, moreover, were positive for human papillomavirus (hpv) dna by polymerase chain reaction (pcr) (79%), and harbored hpv types 6, 16 and 18, in combination. on the other hand, less than 30% of the mainland patients were ... | 1997 | 9361099 |
| human papillomavirus infection in squamous cell carcinoma of the vulva, in various synchronous epithelial changes and in normal vulvar skin. | to investigate the prevalence of human papillomavirus (hpv) infection in various vulvar lesions. | 1997 | 9367704 |
| genetically modified bone marrow-derived dendritic cells expressing tumor-associated viral or "self" antigens induce antitumor immunity in vivo. | the clinical application of synthetic tumor peptide-based vaccines is currently limited to patients with specified major histocompatibility complex (mhc) class i alleles. such logistic limitations may be overcome using tumor gene-based approaches. here we describe the effective generation of dendritic cells (dc) expressing tumor peptide-mhc complexes as a result of particle-mediated transfer of genes encoding tumor-associated antigens (taa). bone marrow-derived dc were transfected with plasmid d ... | 1997 | 9368629 |
| molecular analysis of the interaction between hpv type 16 e6 and human e6-associated protein. | the complex formed between the human papillomavirus type 16 e6 protein and human e6-associated protein, which combine to ubiquitylate and degrade p53, has been studied by chemical crosslinking. analysis of the interactions of proteins purified from escherichia coli as well as proteins expressed in insect cells indicates that, while e6 has the capacity to form dimers, e6 and e6-associated protein interact as two monomers to form a heterologous dimer. | 1997 | 9369221 |
| subungual squamous cell carcinoma in darier's disease. | we report the first case of subungual squamous cell carcinoma in a patient with darier's disease (keratosis follicularis). the presence of human papillomavirus dna was confirmed, consistent with its probable role in carcinogenesis at this site. the role of an altered immune state is discussed as an additional factor. squamous cell carcinomas may be overlooked when they occur among the other cutaneous manifestations and complications of darier's disease. a solitary, ulcerating nail bed lesion war ... | 1997 | 9604454 |
| detection of human papillomavirus dna in condylomata acuminata treated with co2 laser by polymerase chain reaction. | 1997 | 9594330 | |
| [human papillomavirus as an etiological factor in cervical cancer: significance for health care practice]. | 1997 | 9499235 | |
| vestibulodynia--a subset of vulvar vestibulitis or a novel syndrome? | our purpose was to recognize special features of women with both vestibulitis and constant vulvar pain (vestibulodynia) and to determine whether vestibulodynia is a separate disease entity or a subset of vestibulitis. | 1997 | 9423748 |
| destabilization of the rb tumor suppressor protein and stabilization of p53 contribute to hpv type 16 e7-induced apoptosis. | cells that express the human papillomavirus (hpv) type 16 e7 oncoprotein are predisposed to undergo apoptosis. transgenic mice that have e7 expression targeted to either the retinal photoreceptor cells or the lens cells exhibit signs of apoptosis in cells attempting to undergo differentiation. we established a cell culture system to study this process and have determined the domains of e7 that are required for predisposing cells to undergo apoptosis in response to growth arrest signals. regions ... | 1997 | 9426450 |
| human papillomavirus and bladder cancer. | bladder cancer remains an important cause of oncological morbidity and mortality in women. known etiological agents include smoking and exposure to certain industrial chemical compounds, though the origin of the majority of cases remains unknown. human papillomavirus infection is also common in women and has been closely linked to the development of carcinoma of the cervix. it has been suggested that infection with hpv may also be an important factor in the subsequent development of bladder canc ... | 1997 | 9609335 |
| inactivation of p53 increases the cytotoxicity of camptothecin in human colon hct116 and breast mcf-7 cancer cells. | camptothecin (cpt) derivatives are topoisomerase i (top1) inhibitors recently introduced as clinical agents. to explore the role of p53 in cpt-induced cytotoxicity, we examined cpt effects in two isogenic pairs of human cancer cell lines, mcf-7 breast carcinoma and hct116 colon carcinoma cells, in which p53 function had been disrupted by transfection with the human papillomavirus type-16 e6 gene. clonogenic survival assays showed that both mcf-7/e6 and hct116/e6 cells were more sensitive to cpt. ... | 1997 | 9815856 |
| detection of human papillomavirus type 16 dna and of antibodies to human papillomavirus type 16 proteins in children. | we have measured markers of human papillomavirus type 16 (hpv 16) infection in children (1-10 years of age) who were hospitalized for reasons unrelated to papillomavirus infection. genital and buccal swabs obtained from 79 children were tested for the presence of hpv 16 dna by pcr. low-level positivity was found in 34 donors, twice as often in oral than in genital swabs. there was no sex-specific difference, but there was a trend towards a higher positivity rate with young age. serum antibodies ... | 1997 | 9612724 |
| pathology of human immunodeficiency virus infection: noninfectious conditions. | diagnostic anatomic pathologists play a crucial role in the battle against acquired immunodeficiency syndrome (aids). not only are they intimately involved in the treatment of individual patients with human immunodeficiency virus (hiv) infection, but also they make important observations that result in the expansion of the scientific understanding of its pathogenesis. pathologists studying tissue from patients with hiv infection should be familiar with the conditions to which these patients are ... | 1997 | 9869826 |
| in situ pcr to cells and to wax sections. an alternative to in situ hybridization for the detection of low copies of human papillomavirus and cytomegalovirus dna. | 1997 | 8959693 | |
| cellular aging is a critical determinant of primary cell resistance to v-src transformation. | primary cell cultures are in general resistant to the transforming effect of a single oncogene, a finding considered consistent with the multistage theory of carcinogenesis. in the present studies, we examined whether cellular age, differentiation stage, and/or tissue origin of primary cells plays a role in determining their response to v-src transformation. to study the role of cellular age, rat mammary fibroblasts were isolated from a 50-day-old female rat and infected with a recombinant retro ... | 1997 | 8985343 |
| human papillomavirus type influences the extent of chromosomal lag during mitosis in cervical intraepithelial neoplasia grade iii. | the level of risk for carcinoma in the uterine cervix depends on the type of human papillomavirus (hpv) present. we examined whether the hpv type influences the proliferation rate and occurrence of mitotic figures with lagging chromosomes in the precursor of cervical carcinoma. the study group comprised 180 women who were referred because of cytologic changes indicating dysplasia and who were subsequently diagnosed with cervical intraepithelial neoplasia grade iii. the hpv-16-associated lesions ... | 1997 | 8986526 |
| comparison between vaginal tampon and cervicovaginal lavage specimen collection for detection of human papillomavirus dna by the polymerase chain reaction. the canadian women's hiv study group. | the aim of the study was to compare the accuracy of self-administered vaginal tampon (vt) specimens for the detection of human papillomaviruses (hpvs) with that of cervicovaginal lavage specimens (cvl). two hundred seventy-four paired vt and cvl specimens were collected prospectively from women at risk of sexually transmitted diseases. specimens were treated and amplified with the polymerase chain reaction (pcr). each woman served as her own control. one hundred and forty-four of 272 (52.9%) cvl ... | 1997 | 8986948 |
| cervical cancer in young women in taiwan: prognosis is independent of papillomavirus or tumor cell type. | the objective of this study was to address the hypotheses that younger patients with cervical cancer have a uniquely worse clinical outcome and/or are more likely to have adverse tumor cell types or specific human papillomaviruses (hpv). cases of stage ib-iia cervical cancer among women 35 years of age or younger (82) and over 35 (54) were analyzed and compared with respect to the following: (1) histologic type (squamous vs nonsquamous), (2) human papillomavirus (hpv) type via polymerase chain r ... | 1997 | 8995548 |
| comparison of pcr and hybrid capture methods for detection of human papillomavirus in injection drug-using women at high risk of human immunodeficiency virus infection. | we compared hybrid capture, a new technique for detection of human papillomaviruses (hpv), with a pcr assay based on l1 consensus primers. by both methods, the hpv prevalence was higher in human immunodeficiency virus (hiv)-positive women than in hiv-negative women. pcr had a higher sensitivity (0.89 versus 0.48) but lower specificity (0.43 versus 0.93) for detection of pap smear abnormalities, compared to hybrid capture. the higher intensity of hybridization signal by pcr was related to higher ... | 1997 | 9003632 |
| prevalence of single and multiple infection with human papillomaviruses in various grades of cervical neoplasia. | evaluation of human papillomavirus (hpv) diversity in various grades of cervical lesions is helpful for understanding the characteristics of hpv infection in the pathogenesis of cervical neoplasia. a total of 227 women with normal cervices (n = 72), low- and high-grade cervical squamous intraepithelial lesions (sils) (n = 55 and 53, respectively) and cervical carcinomas (n = 47) were screened for human papillomavirus (hpv) types 6, 11, 16 and 18 infection by the polymerase chain reaction. the pr ... | 1997 | 9003746 |
| proteolytic cleavage of human p53 by calpain: a potential regulator of protein stability. | the p53 tumor suppressor protein is activated in cells in response to dna damage and prevents the replication of cells sustaining genetic damage by inducing a cell cycle arrest or apoptosis. activation of p53 is accompanied by stabilization of the protein, resulting in accumulation to high levels within the cell. p53 is normally degraded through the proteasome following ubiquitination, although the mechanisms which regulate this proteolysis in normal cells and how the p53 protein becomes stabili ... | 1997 | 8972227 |
| cytokine production patterns in cervical intraepithelial neoplasia: association with human papillomavirus infection. | genital infection with certain strains of human papillomavirus (hpv) is associated with a high risk of malignant transformation, and hpv-associated cervical intraepithelial neoplasia (cin) can become invasive cancer. host factors are critical in regulating tumor growth, and cytokines that modulate immunologic control may be of particular importance. the type 1 cytokines interleukin 2 (il-2) and interferon gamma (ifn gamma) are immunostimulatory and are thus capable of limiting tumor growth. the ... | 1997 | 9017005 |
| detection of hpv dna in trichilemmomas by polymerase chain reaction. | paraffin sections of 11 formalin-fixed trichilemmomas were investigated for the presence of human papillomavirus (hpv) dna by the polymerase chain reaction (pcr) with the degenerated consensus primer pairs. pcrs were conducted with different annealing temperatures. when the annealing temperature was reduced from 55 degrees c to 50 degrees c, amplification products of the expected size were obtained for all 11 cases investigated. determination of the hpv type was performed by cloning and sequenci ... | 1997 | 9021542 |
| the bovine papillomavirus e6 oncoprotein interacts with paxillin and disrupts the actin cytoskeleton. | the e6 oncoprotein of bovine papillomavirus type 1 (bpv-1) has been shown to transform cells through a p53-independent pathway, but its transforming mechanism is unknown. here we demonstrate in vitro and in vivo interactions between bpv-1 e6 and the focal adhesion protein paxillin. the ability of bpv-1 e6 to complex with paxillin correlated with its ability to transform; e6 mutant proteins impaired in their transformation function also were impaired in their abilities to bind paxillin. e6 bindin ... | 1997 | 9114003 |
| [verrucous carcinoma of the anal margin. the buschke-loewenstein tumor]. | the buschke-loewenstein tumour is a rare variety of squamous cell carcinoma of low-grade malignancy; it is located in the external genital organs and the perianal region. we present a new case of this uncommon tumour of short evolution in a 73 years old male patient. resection was the only treatment, without relapse at 12 months. a review of the literature shows only 13 cases previously published; we comment the human papillomavirus role in their pathogenesis and the various therapies proposed, ... | 1997 | 9115819 |
| the human papillomavirus type 16 e7 protein is associated with the nucleolus in mammalian and yeast cells. | in this study we show, by immunofluorescence and electron microscopy immuno-gold labelling, that the major transforming protein of human papillomavirus type 16 e7 is associated with the nucleolus of cells derived from the hpv16-positive cervical carcinoma line caski. the e7 nucleolar staining appeared to be cell cycle dependent, being considerably reduced in the g2 phase. the total level of the protein in the cell, however, remained constant during all phases. we also show that the cellular prot ... | 1997 | 9121762 |
| variable transduction efficiency of murine leukemia retroviral vector on mammalian cells: role of cellular glycosylation. | to elucidate the cellular tropism of moloney murine leukemia virus (mulv), we have studied the transduction efficiency of a recombinant mulv vector carrying the beta-galactosidase reporter gene on a variety of rodent cells. under optimal conditions for in vitro cell transduction, primary cultures of adult rat fibroblasts derived from various organs were very poorly transduced by the ecotropic mulv vector (0.02-0.12%) when compared to immortalized cells such as the f2408 (42%) and 3y1 (defined as ... | 1997 | 9123877 |
| correlation between the presence of anti hpv33 vlp antibodies and hpv dna in cervical neoplasia patients. | the l1 capsid proteins derived from human papillomavirus (hpv) type 33 were expressed in a recombinant baculovirus system using sf9 insect cells. selected sera originating in women from case-control study carried out in spain and colombia found negative and positive for hpv16, 18, 31, 33 and 35 dna were tested in elisa for the presence of igg antibodies to purified virus-like particles (vlp). the reactivity was type-restricted with the possible exception of hpv31. | 1997 | 9125054 |
| identification of human papillomavirus types 16 and 18 deoxyribonucleic acid sequences in bulky cervical cancer after chemotherapy. | the objectives of this study were to evaluate the effect of chemotherapy on the continual presence of human papillomavirus deoxyribonucleic acid sequences in bulky cervical cancer tissues and the relationship between the presence of human papillomavirus and the response of these patients to chemotherapy. | 1997 | 9125612 |
| successful treatment of a generalized human papillomavirus infection with granulocyte-macrophage colony-stimulating factor and interferon gamma immunotherapy in a patient with a primary immunodeficiency and cyclic neutropenia. | we describe a patient with generalized verrucosis secondary to human papillomavirus (hpv) type 2 infection and a primary immunodeficiency and cyclic neutropenia. treatment, which was well tolerated, included granulocyte-macrophage colony-stimulating factor and interferon gamma (ifn-gamma). in vitro assays to assess responses of t lymphocytes to mitogens (ie, proliferation assay and ifn-gamma enzyme-linked immunosorbent assay) were performed. in situ hybridization and polymerase chain reaction we ... | 1997 | 9126013 |
| aggressive giant condyloma acuminatum associated with oncogenic human papilloma virus: a case report. | malignant transformation has been described in 30% of cases of giant anorectal condyloma acuminatum. the authors report on a 33-year-old man who was heterosexual and hiv negative and who had a giant anal condyloma. despite aggressive therapy with multiple fulgurations, interferon alpha and isotretinoin, an invasive squamous cell carcinoma of the rectum developed. an abdominoperineal resection was done followed by radiotherapy and chemotherapy, but this treatment regimen was unsuccessful in contr ... | 1997 | 9126130 |
| analysis of the interaction between human papillomavirus type 16 e7 and the tata-binding protein, tbp. | the e7 protein encoded by human papillomavirus type 16 shows transforming and immortalizing activities which are mediated, in part, through the interaction of the viral oncoprotein with the prb protein family. this interaction is not solely responsible for e7 function, however, and other properties of e7, such as the interaction with basal transcription factors such as tbp, are likely to be of importance. we show here that three regions of the viral protein contribute to the interaction between ... | 1997 | 9129664 |
| human papillomavirus dna in urine specimens of men with condyloma acuminatum. | because warts are often found in the male urethra, human papillomavirus (hpv) may well be present in urine of patients with urethral condylomata. | 1997 | 9132984 |
| demonstration of antibodies against human papillomavirus type-11 e6 and l2 proteins in patients with recurrent respiratory papillomatosis. | recurrent respiratory papillomatosis (rrp) is highly prevalent in thailand, with the human papillomavirus (hpv) type-11 being the most widespread. in this study, we isolated the hpv type-11 (hpv-11) genome from subjects with rrp and subcloned the e6 and l2 open reading frames (orfs) with the expression vectors pex1 and pex3. the recombinant e6/beta-gal and l2/beta-gal fusion proteins were expressed in e. coli. using the recombinant proteins, we demonstrated the presence of antibodies against hpv ... | 1997 | 9134142 |
| abrogation of p53 function by hpv16 e6 gene delays apoptosis and enhances mutagenesis but does not alter radiosensitivity in tk6 human lymphoblast cells. | in order to gain a better understanding of the role of p53 in radiation-induced mitotic failure, apoptosis and mutagenesis, we introduced the hpv16 e6 gene via a retroviral vector into the tk6 human lymphoblast cell line which expresses wild type p53. abrogation of p53 function by e6 resulted in a delayed and reduced apoptotic response and a moderate increase in the frequency of mutations at the thymidine kinase (tk) locus following gamma-irradiation, but failed to alter radiosensitivity. the ap ... | 1997 | 9135067 |
| failure of hpv e6 to rapidly degrade p53 in human hela x pnet cell hybrids. | the ability of the e6 protein from high risk human papillomaviruses (hpvs) to degrade p53 via the ubiquitin pathway plays a major role in the development of cervical carcinomas. we have previously generated cell hybrids between a p53 null peripheral neuroepithelioma (pnet) cell line and a cervical carcinoma hela cell line which exhibits efficient e6-mediated degradation of p53. all of the resulting hybrids expressed hpv 18 e6 from the hela parent and some of the hybrids additionally expressed hp ... | 1997 | 9135068 |
| sensitive in situ hybridization with catalyzed reporter deposition, streptavidin-nanogold, and silver acetate autometallography: detection of single-copy human papillomavirus. | the usefulness of standard in situ hybridization for viral nucleic acid detection is occasionally limited by its sensitivity limit of 10 to 50 copies per cell. a modified version of the recently described signal amplification method, catalyzed reporter deposition (card), and its application to formalin-fixed cells and tissue sections is presented. deposition of the reporter is facilitated by using horseradish peroxidase catalyzing the deposition of biotinylated tyramide on the location of the pr ... | 1997 | 9137082 |
| p53, waf1/cip1 and mdm2 expression in skin lesions associated with human papillomavirus and human immunodeficiency virus. | human papillomaviruses (hpvs) express various gene products, such as e6 protein which complexes with the p53 tumor suppressor protein and therefore diminishes p53-related regulatory mechanisms. this interaction is assumed to be hpv type-specific as "high risk" or oncogenic hpv types have more affinity for p53 binding than their "low risk" or non-oncogenic counterparts. furthermore, hiv infection is believed to activate latent hpv infection and transcription via direct and indirect interaction wi ... | 1997 | 9137486 |
| p53 alterations but no human papillomavirus infection in preinvasive and advanced squamous esophageal cancer in italy. | geographic differences in exposure to suspected carcinogens have been identified in esophageal carcinogenesis, and both p53 alterations and human papillomavirus (hpv) infection have been reported in esophageal squamous carcinoma (esc) from high-risk areas, including china and south africa. the status of p53 alterations and hpv infection in esc has not been determined in northern italy, where the incidence of esc is low. formalin-fixed paraffin-embedded esophageal samples containing normal, dyspl ... | 1997 | 9138659 |
| cytogenetics of carcinoma of the cervix uteri: a review. | cytogenetic studies on carcinoma of the cervix have shown the nonrandom involvement in structural changes of a number of chromosomes, particularly chromosomes 1, 3, 5, 11, and 17. apart from chromosome 5, where a short-arm isochromosome is the commonest derivative, these chromosomes most often undergo short-arm deletions. notably, chromosome 17 may have undergone structural changes that result in loss of the tumor suppressor gene tp53 on 17p; chromosomal translocations may in some tumors perform ... | 1997 | 9140451 |
| cell mediated immunity induced in mice by hpv 16 l1 virus-like particles. | recombinant human papillomavirus (hpv) type 16 l1 virus-like particles (vlps) expressed in the baculovirus system were used to investigate the cellular immune response to human papillomavirus type 16. the cell-mediated immune response was evaluated through immunization of mice with hpv 16 l1 virus-like particles using a lymphoproliferation assay and cytokine production and cytometric analysis of lymphocyte subsets. a significant proliferative response was observed which was associated with secre ... | 1997 | 9140917 |
| mechanism of p53 degradation. | 1997 | 9141469 | |
| human papillomavirus-associated tumors of the skin and mucosa. | this review discusses diseases of the lower genital tract in which the presence of human papillomavirus (hpv) dna is well documented. we discuss epidemiologic, clinical, and experimental data supporting a causative role for hpv in mucosal malignancies, with emphasis on the role of viral and host factors in their pathogenesis. of special interest is the recently discovered association of cutaneous tumors with hpvs, previously known only for tumors in epidermodysplasia verruciformis (ev). the freq ... | 1997 | 9146528 |
| sensitive chemiluminescence in situ hybridization for the detection of human papillomavirus genomes in biopsy specimens. | we developed a sensitive chemiluminescence in situ hybridization assay for detection of human papillomavirus (hpv) dna for objective and semiquantitative evaluation of the results. the hybridization reaction was performed using either digoxigenin-, biotin-, or fluorescein-labeled probes, visualized with alkaline phosphatase as the revealing enzyme and a highly sensitive 1,2 dioxetane phosphate as chemiluminescent substrate. the light emitted from the hybridized probes was detected, analyzed, and ... | 1997 | 9154160 |