Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| the combined use of chemical and biochemical markers to assess water quality in two low-stream rivers (ne spain). | carps (cyprinus carpio) and red swamp crayfish (procambarus clarkii) were sampled from two lowstream mediterranean rivers (anoia and cardener) receiving extensive urban and industrial waste water discharges. tissue residues of selected pollutants (organochlorinated compounds, polycyclic aromatic hydrocarbons (pahs)) were determined in conjunction with different biochemical responses (cytochrome p450, phase ii enzymes) with the aim of investigating whether resident organisms were responsive to ch ... | 2002 | 12483808 |
| a comparison of trace element concentrations in cultured and wild carp (cyprinus carpio) of lake kasumigaura, japan. | the concentrations of 13 elements were determined in the muscle, liver, intestine, kidney, and gonads of cultured and wild carp caught at two sites in lake kasumigaura, japan, between september 1994 and september 1995. despite having a reputation for being heavily polluted, the carp were not heavily burdened with metals. our results suggest that despite their dietary differences, the wild and cultured fish were accumulating and distributing metals in the same manner and that aquaculture practice ... | 2002 | 12485577 |
| ligula intestinalis--a tapeworm contraceptive. | human contraceptives are 'big business', but might the real breakthrough come out from the pharmaceutical industry but from a tapeworm? ligula intestinalis can induce infertility in infected fish of the carp family - both males and females. if the mechanism for this can be discovered, this humble flatworm could drastically change contraceptive practices with one pill for all. | 2002 | 12486303 |
| structural dependencies of h3jnc' scalar coupling in protein h-bond chains. | the h-bond ((h3)j(nc')) and peptide bond ((1)j(nc')) scalar couplings establish connectivity of the electronic structure in the h-bond chains of proteins. the correlated changes of (h3)j(nc') and (1)j(nc') couplings extend over several peptide groups in the chains. consequently, the electronic structure of the h-bond chains can affect (h3)j(nc') in a manner that is independent of the local h-bond geometry. by taking this into account, and by using a more complete set of h-bond geometry parameter ... | 2002 | 12440921 |
| phenotypic characteristics and pathogenicity of aeromonas genomospecies isolated from common carp (cyprinus carpio l.). | to evaluate the relationship between the genomospecies, phenotypic profile and pathogenicity for carp of 37 motile aeromonas strains. | 2002 | 12452960 |
| isolation, expression and characterization of carp retinol-binding protein. | vitamin a alcohol and its precursors carotenoids are introduced in the organism with the diet, transported to the liver and from there as retinol to target tissues by a specific carrier, the retinol-binding protein (rbp). rbp, isolated and characterized in many vertebrates, shows very high homology among the species investigated; however, very little is known in fish. in the present work rbp cdna isolated from a carp liver library was transcribed and translated in vitro and the corresponding pro ... | 2002 | 12354658 |
| efficacy of water hyacinth compost in nursery ponds for larval rearing of indian major carp, labeo rohita. | the use of water hyacinth compost as manure in nursery ponds for larval rearing of indian major carp, labeo rohita was studied. better performance with regard to growth and survivability of the larvae was recorded in the ponds treated with water hyacinth compost (group a) than in either the ponds treated with inorganic fertilizers (group b) or the ponds where no treatment was applied (group c). the average percentages of survival in groups a, b and c were 14.3, 11.2 and 5.0, respectively. thus a ... | 2002 | 12365499 |
| endocrine disruptors in sewage treatment plants, receiving river waters, and sediments: integration of chemical analysis and biological effects on feral carp. | occurrence of alkylphenol ethoxylates or their metabolites (alkylphenols and carboxylated derivatives), as well as natural and synthetic steroids in sewage treatment plant (stp) effluents and in their receiving waters, has been related to biological effects, measured as alterations of plasma vitellogenin (vtg) concentration in natural fish populations. water composites of stp influents, effluents, sludge, river water, sediment, and feral carps (cyprinus carpio) were analyzed over a seven-month p ... | 2002 | 12371491 |
| in vitro antiestrogenic effects of aryl methyl sulfone metabolites of polychlorinated biphenyls and 2,2-bis(4-chlorophenyl)-1,1-dichloroethene on 17beta-estradiol-induced gene expression in several bioassay systems. | methylsulfonyl (meso(2)) metabolites of polychlorinated biphenyls (pcbs) and 2,2-bis(4-chlorophenyl)-1,1-dichloroethene (4,4'-dde), itself a metabolite of the insecticide 4,4'-ddt, are emerging as a major class of contaminants in the tissues of wildlife and humans. we investigated the antiestrogenic capacity and potencies of 3'- and 4'-meso(2)-2,2',4,5,5'-pentachlorobiphenyl (cb101) and -2,2',4,5'-tetrachlorobiphenyl (cb49), which are among the most environmentally persistent meso(2)-pcbs, and 3 ... | 2002 | 12377985 |
| relationships between persistent organic chemicals residues and biochemical constituents in fish from a protected area: the french national nature reserve of camargue. | the reserve of biosphere of camargue [french national nature reserve of camargue (nnrc)] is a protected area frequently exposed to natural and anthropogenic environmental alterations. to evaluate potential contamination of fish with lipophilic chemicals-organochlorines (ocs) and polycyclic aromatic hydrocarbons (pahs)-a biological monitoring survey was carried out. metabolic reserve levels were evaluated to select appropriate biological indicators able to be significant biomarkers. in addition, ... | 2002 | 12379424 |
| primary structure of myosin heavy chain from fast skeletal muscle of chum salmon oncorhynchus keta. | the nucleotide sequence of the cdna encoding myosin heavy chain of chum salmon oncorhynchus keta fast skeletal muscle was determined. the sequence consists of 5,994 bp, including 5,814 bp of translated region deducing an amino acid sequence of 1,937 residues. the deduced sequence showed 79% homology to that of rabbit fast skeletal myosin and 84-87% homology to those of fast skeletal myosins from walleye pollack, white croaker and carp. the putative binding-sites for atp, actin and regulatory lig ... | 2002 | 12381388 |
| alpha(1)-adrenergic activation of the cardiac ankyrin repeat protein gene in cardiac myocytes. | cardiac ankyrin repeat protein (carp) is a nuclear transcription cofactor that is activated by multiple signaling pathways in hypertrophic cardiac myocytes. since carp has been reported to be a transcriptional co-repressor, its activation during hypertrophy might contribute to the deregulation of gene expression leading to heart failure. here, we found that alpha(1)-adrenergic signaling activates carp mrna expression in rat cardiac myocytes. to examine how alpha(1)-adrenergic signaling activates ... | 2002 | 12384280 |
| in vitro assessment of potential mechanism-specific effects of polybrominated diphenyl ethers. | this study examined the ability of environmentally relevant concentrations of 10 different polybrominated diphenyl ethers (pbdes) to induce aryl hydrocarbon receptor (ahr)- and estrogen receptor (er)-mediated gene expression in vitro. it also examined the ability of pbdes to displace steroid hormones from serum proteins in vitro. at concentrations ranging up to 880 ng/ ml, none of the pbdes significantly displaced tritiated 17beta-estradiol (e2) or testosterone from hormone-stripped carp serum. ... | 2002 | 12389923 |
| cloning and sequencing of wee1 from the ovary of the gynogenetic japanese silver crucian carp (ginbuna) [carassius auratus langsdorfi]. | wee1 is one of the genes which regulates the cell cycle. cdna encoding a wee1-homolog has been isolated from japanese silver crucian carp (ginbuna) by using polymerase chain reaction (pcr) techniques. the cdna obtained from the ovary tissue encoded a protein of 526 amino acids and the deduced amino acid sequence showed high homology (69.9%) with xenopus laevis wee1 in the protein kinase domain. a phylogenetic analysis among wee1 amino acid sequences from various organisms showed that the crucian ... | 2002 | 12391727 |
| seasonal changes in plasma steroid levels in indian major carp labeo rohita: influence of homologous pituitary extract on steroid production and development of oocyte maturational competence. | circannual variations in plasma levels of testosterone (t), 17beta-estradiol (e(2)), and 17 alpha, 20 beta-dihydroxy-4-pregnen-3-one (dhp) as well as seasonal fluctuations in ovarian steroid synthetic potential were observed in indian major carp, labeo rohita. a study was also conducted to examine the mechanism of the development of gonadotropin-induced maturational competence in oocytes of this fish. the present study recorded the lowest values of plasma e(2) and t in l. rohita during the perio ... | 2002 | 12392685 |
| glandular kallikreins in the teleost cyprinus carpio: tissue distribution, possible involvement in prolactin processing, and effect of 17 beta-estradiol in vivo. | we examined glandular kallikrein (gk), a putative prolactin processing protease, in the teleost cyprinus carpio. when employing an anti-centropristis striata gk antibody proteins of 39 kda in muscle, 52 kda in gill, 52 kda in kidney, and two proteins of 46 and 72 kda in pituitary gland were detected. immunoreactive kallikreins were recognized in intermuscle cell tissue, epithelial gill cells, apical region of tubular cells, and prolactin producing lactotrophs in pituitary gland, suggesting a osm ... | 2002 | 12392686 |
| high vitamin a content in some small indigenous fish species in bangladesh: perspectives for food-based strategies to reduce vitamin a deficiency. | recognising the importance of fish in the bangladeshi diet, the objective of the present study was to screen commonly consumed fish species for vitamin a content to evaluate the potential of fish as a vitamin a source in food-based strategies to combat vitamin a deficiency. samples of 26 commonly consumed fish species and one crustacean were collected in kishoreganj and mymensingh, bangladesh. to obtain edible parts, the fish were cleaned by bangladeshi women according to traditional practices. ... | 2002 | 12396468 |
| characterisation of a monoclonal antibody to carp il-1beta and the development of a sensitive capture elisa. | a carp il-1beta gene was identified from a subtraction hybridisation technology based cdna library from activated carp leucocytes. this gene was cloned into pqe vector carrying 6xhis tag and the protein was expressed. recombinant il-1beta was used to produce hybridomas specific for carp il-1beta. monoclonal antibodies were purified by affinity column and a sandwich elisa for il-1beta was developed with a detection limit of 10 ng of the recombinant protein. using the capture elisa, the presence o ... | 2002 | 12400859 |
| granulocyte responses to experimental injection of live and formalin-killed bacteria in carp (cyprinus carpio). | the objective of the study was to investigate the dynamics of changes in number of granulocytes in bacterial infections of carp (cyprinus carpio). carp were inoculated with non-pathogenic or pathogenic bacteria and changes in type i (neutrophils) and type ii granulocyte (basophils/eosinophils) counts in kidney, circulating blood and peritoneal cavity were assessed. after the injection of non-pathogenic bacteria (escherichia coli), the number of type i and ii cells in blood increased after 6-12h, ... | 2002 | 12406659 |
| [effects of pituitary adenylate cyclase activating polypeptide (pacap) on intracellular camp and ca(2+) levels in common carp (cyprinus carpio) pituitary cells in vitro]. | in order to study signal transduction in pacap-stimulated gh release, effects of ovine pacaps (opacaps) on camp synthesis and intracellular free ca(2+)([ca(2+)](i)) levels in primarily cultured common carp (cyprinus carpio) pituitary cells were investigated using radioimmunoassay (ria) for camp and [ca(2+)](i) indo-1/am loading techniques. the main results obtained could be briefly summarized as follows: parallel with their gh-releasing effects, opacap-38 (0.01 nmol/l to 1 micromol/l) and opacap ... | 2002 | 12417926 |
| gonadosomatic index and testis morphology of common carp (cyprinus carpio) in rivers contaminated with estrogenic chemicals. | to study the effect of estrogenic chemicals on fish, the gonadosomatic index (gsi = [testis weight/body weight] x 100) and testis histology of mature common carp (cyprinus carpio) from 2 contaminated sites (ishizu and wada rivers, osaka) and a control site were examined between june 1998 and march 2001. the concentration of nonylphenol, bisphenol a and 17beta-estradiol in the ishizu river was 3-4 times higher than in the wada river. in the pre-breeding and breeding seasons, there were no signifi ... | 2002 | 12419869 |
| immune modulation by fish kinetoplastid parasites: a role for nitric oxide. | trypanoplasma borreli and trypanosoma carassii are kinetoplastid parasites infecting cyprinid fish. we investigated the role of nitric oxide (no) in immune modulation during t. borreli and t. carassii infection of carp. phagocytic cells from different organs produced no and serum nitrate levels increased, demonstrating that t. borreli activates no production in vivo. in contrast, t. carassii did not induce no production in vivo and inhibited lps-induced no production in vitro. production of no w ... | 2002 | 11817378 |
| heterologous exchanges of the glycoprotein and the matrix protein in a novirhabdovirus. | infectious hematopoietic necrosis virus (ihnv) and viral hemorrhagic septicemia virus (vhsv) are two salmonid rhabdoviruses replicating at low temperatures (14 to 20 degrees c). both viruses belong to the novirhabdovirus genus, but they are only distantly related and do not cross antigenically. by using a recently developed reverse-genetic system based on ihnv (s. biacchesi et al., j. virol. 74:11247-11253, 2000), we investigated the ability to exchange ihnv glycoprotein g with that of vhsv. thu ... | 2002 | 11861855 |
| the effect of dietary immunomodulation upon edwardsiella tarda vaccination in healthy and immunocompromised indian major carp (labeo rohita). | in order to determine the impact on disease resistance of four dietary immunomodulators viz., beta-1,3 glucan, levamisole, vitamins c and e, in an important farmed indian major carp species, rohu (labeo rohita ham.), fish were fed diets containing various levels of these substances during a 60 day trial. aflatoxin b1 (afb,) at 125 mg kg(-1) body weight was injected intraperitoneally (i.p.) into fish to induce an immunosuppressive state on the first day of the experiment in some individuals. the ... | 2002 | 11866127 |
| molecular cloning of growth hormone-encoding cdna of an indian major carp, labeo rohita, and its expression in escherichia coli and zebrafish. | the cdna clones encoding for growth hormone (gh) of an indian major carp rohu labeo rohita were isolated from a cdna library constructed from the poly(a)(+) rna extracted from the pituitary glands of rohu. partial gh cdna of the rohu (3'-end) was amplified by rt-pcr and used as probe to screen the cdna library. full-length gh-specific cdna clones (1180 bp) were isolated and sequenced. the sequence contains 48-bp 5'-noncoding region followed by an orf of 621 bp and a 3'-noncoding region of 521 bp ... | 2002 | 11884069 |
| isolation and characterization of a homologue of mammalian prolactin-releasing peptide from the tilapia brain and its effect on prolactin release from the tilapia pituitary. | in the tilapia (oreochromis mossambicus), as in many teleosts, prolactin (prl) plays a major role in osmoregulation in freshwater. recently, prl-releasing peptides (prrps) have been characterized in mammals. independently, a novel c-terminal rf (arginine-phenylalanine) amide peptide (carrasius rf amide; c-rfa), which is structurally related to mammalian prrps, has been isolated from the brain of the japanese crucian carp. the putative prrp was purified from an acid extract of tilapia brain by af ... | 2002 | 11884078 |
| zebrafish as a model for vertebrate reproduction: characterization of the first functional zebrafish (danio rerio) gonadotropin receptor. | vertebrate reproduction is tightly regulated by conserved glycoprotein hormones produced by the pituitary gland. follicle-stimulating hormone (fsh) in tetrapods and gonadotropic hormone i (gth-i) in fishes are orthologous glycoprotein hormones that control the timing of egg production and the number of eggs produced. zebrafish, a well-established genetic model for developmental biology, also offers potential advantages for studies of reproductive toxicology, especially for modeling the impact of ... | 2002 | 11884080 |
| cloning of a novel rainbow trout (oncorhynchus mykiss) cc chemokine with a fractalkine-like stalk and a tnf decoy receptor using cdna fragments containing au-rich elements. | an activation-specific cdna library was made from phytohaemagglutinin (pha)-activated haematopoietic cells of the rainbow trout (oncorhynchus mykiss) using the technique of suppression subtractive hybridization. several immune system genes were identified, including an interleukin (il)1 receptor related protein and two invariant chain-like proteins. many clones showed no similarity by blast search, but had au-rich elements. these fragments were labelled and used for hybridization with a pha-acti ... | 2002 | 11886174 |
| role of nitric oxide in control of light adaptive cone photomechanical movements in retinas of lower vertebrates: a comparative species study. | the possible role of nitric oxide (no) as a novel light adaptive neuromodulator of cone plasticity (photomechanical movements) in retinae of two contrasting species of fish (freshwater carp and marine bream) and an example of an amphibian (xenopus laevis) was studied pharmacologically by cytomorphometric measurements. application of a no donor [s-nitroso-n-acetyl-d, l-penicillamine] (500-700 microm) to dark-adapted retinae induced contraction of cones with an efficiency (ce) relative to full lig ... | 2002 | 11890744 |
| upper extremity infections following common carp fish (cyprinus carpio) handling. | upper extremity infection caused by aquatic pathogens on fish is a well recognized clinical entity. we report five consecutive cases of upper extremity infections, ranging from a simple localized reaction to a life-threatening systemic illness, which developed after handling common carp fish (cyprinus carpio). in four cases, infection occurred following a penetrating injury by either the bones or the fin spines of the fish. vibrio vulnificus was isolated from wound aspirates in four cases. early ... | 2002 | 11895351 |
| determination of the complete genomic sequence and analysis of the gene products of the virus of spring viremia of carp, a fish rhabdovirus. | the complete genome of spring viremia of carp virus (svcv) was cloned and the sequence of 11019 nucleotides was determined. it contains five open reading frames (orf's) encoding for the nucleoprotein n; phosphoprotein p; matrix protein m; glycoprotein g; and the viral rna dependent rna polymerase l. genes are organised in the order typical for rhabdoviruses: 3'-n-p-m-g-l-5'. the short leader and trailer regions of svcv exhibit inverse complementarity and are similar to the respective 3' and 5' e ... | 2002 | 11900842 |
| cloning and expression pattern of the lysozyme c gene in zebrafish. | here, we report isolation and developmental expression pattern of the zebrafish lysozyme c gene. amino acid sequence analysis showed that the zebrafish lysozyme c protein shared approximately 37-80% identities with the mouse, human, chicken, and carp counterparts. whole-mount in situ hybridization showed that the lysozyme c gene was expressed in macrophages, as its expression was co-localized with the known myeloid lineage markers l-plastin and pu.1. at 20 hours postfertilization (hpf), most of ... | 2002 | 11900976 |
| the epizootic haematopoietic necrosis virus (iridoviridae) induces apoptosis in vitro. | the epizootic haematopoietic necrosis virus (ehnv) is an iridovirus causing severe disease in different fish species. we investigated the induction of apoptosis during ehnv infection of the epithelioma carp papulosum (epc) cell line. apoptosis reveals several characteristic morphological changes, such as chromatin condensation, nuclear fragmentation, cytoplasm membrane disorientation, or mitochondrial changes. during ehnv infection of epc cells the occurrence of apoptosis was analysed using a fl ... | 2002 | 11911588 |
| freshwater pollution biomarker: response of brain acetylcholinesterase activity in two fish species. | the effect of prolonged exposure at two sites along the reconquista river (argentina), a highly polluted peri-urban water body, on brain acetylcholinesterase (ache, ec 3.1.1.7, acetylcholine acetylhydrolase) of two teleosts was examined. caged cyprinus carpio and field-captured cnesterodon decemmaculatus were used as sentinel organisms. eserine concentration inhibiting 50% of ache activity (ic50) and inhibition kinetic parameters were also evaluated. interspecies ic50 differences were found to a ... | 2002 | 11912052 |
| expression, purification and dna-binding activity of tilapia muscle-specific transcription factor, myod, produced in escherichia coli. | myod is one of several helix-loop-helix proteins regulating muscle-specific gene expression. using a reverse transcription-polymerase chain reaction, 5'-rapid cdna end amplification, and plaque hybridization, myod cdna was cloned from the mrna of tilapia dorsal skeletal muscle. the 1015 bp myod cdna product contained an 846 bp open reading frame with flanking regions of 115 and 64 bp at the 5'- and 3'-ends, respectively. results showed that the tilapia myod sequence, which includes one polypepti ... | 2002 | 11923092 |
| novel aeromonas hydrophila ppd134/91 genes involved in o-antigen and capsule biosynthesis. | the sequences of the o-antigen and capsule gene clusters of the virulent aeromonas hydrophila strain ppd134/91 were determined. the o-antigen gene cluster is 17,296 bp long and comprises 17 genes. seven pathway genes for the synthesis of rhamnose and mannose, six transferase genes, one o unit flippase gene, and one o-antigen chain length determinant gene were identified by amino acid sequence similarity. pcr and southern blot analysis were performed to survey the distribution of these 17 genes a ... | 2002 | 11953367 |
| [effect of bacterial infection on the lipid composition of carp blood lymphocytes]. | the initial stage of bacterial infection is characterized by an increase in the level of total lipids and polyenoic fatty acids in membrane phospholipids of blood lymphocytes of carp (cyprinus carpio l.). in the fish infected with aeromonads, changes in the ratios of fatty acids in phospholipids are similar for either lymphocytes, liver, or whole blood. the extent to which these changes are pronounced depends on the original physiological status of the fish. | 2002 | 11962218 |
| recombinant carp parvalbumin, the major cross-reactive fish allergen: a tool for diagnosis and therapy of fish allergy. | ige-mediated reactions to fish allergens represent one of the most frequent causes of food allergy. we have constructed an expression cdna library from carp (cyprinus carpio) muscle in phage lambda gt11 and used serum ige from a fish allergic patient to isolate 33 cdna clones that coded for two parvalbumin isoforms (cyp c 1.01 and cyp c 1.02) with comparable ige binding capacities. both isoforms represented calcium-binding proteins that belonged to the beta-lineage of parvalbumins. the cyp c 1.0 ... | 2002 | 11971005 |
| aquacultural and socio-economic aspects of processing carps into some value-added products. | carps are the mainstay of indian aquaculture, contributing over 90% to the total fish production, which was estimated to be 1.77 million metric tonnes in 1996. carp culture has a great potential for waste utilization and thus for pollution abatement. many wastes such as cow, poultry, pig, duck, goat, and sheep excreta, biogas slurry, effluents from different kinds of factories/industries have been efficiently used for enhancing the productivity of natural food of carps and related species. besid ... | 2002 | 11991079 |
| short-term responses of oryzias latipes (pisces: adrianichthyidae) and macrobrachium nipponense (crustacea: palaemonidae) to municipal and pharmaceutical waste water in beijing, china: survival, behaviour, biochemical biomarkers. | whole effluent toxicity was assessed for the fish oryzias latipes and the prawn macrobrachium nipponense for 18 h in a dilution series (0-66%) of the inflow and effluent of a municipal waste water treatment plant as well as waste water from a teramycin producing pharmaceutical industry, before, during and after a pilot laboratory purification process. the waste waters caused acute toxicity as measured by inhibition of light emission in the luminiscent bacterium vibrio qingaiensis sp. nov. (q67). ... | 2002 | 11996134 |
| combined effects of cadmium and composted manure to aquatic organisms. | to evaluate the interactive toxicity of cadmium (cd) and composted manure to aquatic organisms 96 h static bioassays were conducted in the laboratory with fry of common carp (cyprinus carpio), copepod (diaptomusforbesi) and oligochaete worm (branchiura sowerbyi). five concentrations of composted manure (0, 0.25, 0.5, 1.0 and 6.7 g/l) were prepared from the aquatic weed, pistia stratiotes and each of them was combined with several concentrations of cd to determine 96 h lc-50 values of cd for the ... | 2002 | 11999773 |
| carp, a cardiac ankyrin-repeated protein, and its new homologue, arpp, are differentially expressed in heart, skeletal muscle, and rhabdomyosarcomas. | arpp, a protein containing an ankyrin repeat domain, pest sequence, and proline-rich region, is a novel ankyrin-repeated protein highly homologous to carp, which is proposed to be the putative genetic marker for cardiac hypertrophy. in this study, we comparatively analyzed expression of arpp and carp protein in skeletal and cardiac muscles and rhabdomyosarcomas (rmss). in adult skeletal muscle, arpp was preferentially expressed in the nucleus and cytoplasm of type i fibers, whereas carp was bare ... | 2002 | 12000728 |
| arpp, a new homolog of carp, is preferentially expressed in type 1 skeletal muscle fibers and is markedly induced by denervation. | in this study, we isolated and characterized a murine counterpart of the human arpp (harpp) gene. sequence analysis revealed that the murine arpp (marpp) gene is almost identical to the ankrd2 gene, which has recently been isolated as a mouse gene induced in stretched skeletal muscle. the marpp gene encodes a protein of 332 amino acids that contains four well-conserved ankyrin-repeat domains in the central portion of the protein. the amino acid sequence of marpp protein (marpp) is highly homolog ... | 2002 | 12004005 |
| vitellogenin induction in cyprinus carpio treated with 17beta-estradiol and 4-nonylphenol. | the aim of the present study was to demonstrate induction of vitellogenin in the common carp (cyprinus carpio) as a biomarker for monitoring freshwater ecosystems. sexually undifferentiated specimens of common carp were treated experimentally with 17beta-estradiol and increasing doses of 4-nonylphenol and levels of plasma vtg were measured in order to: 1) validate an elisa assay for plasma vtg in the common carp using the polyclonal rabbit anti-salmon vtg antibody aa1 (biosense, norway); 2) chec ... | 2002 | 12004977 |
| initial characteristics of koi herpesvirus and development of a polymerase chain reaction assay to detect the virus in koi, cyprinus carpio koi. | since 1998, episodes of mass mortality have occurred in populations of common carp cyprinus carpio carpio in israel and in populations of koi cyprinus carpio koi in israel and the usa. a herpesvirus isolated from infected fish has been shown in experimental studies to induce disease and mortality similar to those observed in outbreaks at infected farms. initial characteristics of the virus show that it is clearly different from herpesvirus cyprini (chv), the most commonly known herpesvirus from ... | 2002 | 12005231 |
| pcr-cloning and gene expression studies in common carp (cyprinus carpio) insulin-like growth factor-ii. | insulin-like growth factor-ii (igf-ii) is a member of a growth factor family related to fetal growth in mammals but its physiological role has not been clearly identified in fish. in teleosts, the basic mechanism of the growth hormone (gh)-igf axis is known to be operative but in a different manner. for instance, igf-i exhibits gh dependence whereas for igf-ii, its gh dependence varies in different fish species. in this study, we used polymerase chain reaction (pcr) to obtain a common carp igf-i ... | 2002 | 12020820 |
| the immune responses of common carp, cyprinus carpio l., injected with carp interleukin-1beta gene. | the function of the common carp, cyprinus carpio l., interleukin-1beta (il-1beta) gene was studied by dna injection. to investigate the immune responses to il-1beta, a plasmid construct of cytomegalovirus (cmv)-driven carp il-1beta was injected into the epaxial muscle of carp. il-1beta protein expressed in serum on 1, 3, and 5 days after plasmid injection was quantified by elisa and immunohistochemistry. il1-beta gene injection increased proliferation of the lymphocytes by phytohemagglutinin (ph ... | 2002 | 12034023 |
| nuclear transplantation of somatic cells of transgenic red carp (cyprinus carpio haematopterus). | nuclear transplantation was performed using kidney cells, tail fin cells and cultured tail fin cells of 18th passage of the f4 hgh gene transferred red carp (cyprinus carpio haematopterus) as donors and the eggs of loach (misgurnus anguillicaudatus) of huanghe carp (cyprinus carpio haematopterus) as recipients. using loach unfertilized eggs as recipients 0.33% of reconstructed embryos developed in to neural plate from kidney cells, and 0.1% of reconstructed embryos developed in to muscular react ... | 2002 | 12043567 |
| cardiac ankyrin repeat protein, a negative regulator of cardiac gene expression, is augmented in human heart failure. | the technique of representational difference analysis of cdna has been applied to screen for differentially expressed genes in a canine model of pacing-induced heart failure. we identified the canine homolog of the cardiac ankyrin repeat protein (carp) which has been shown to be involved in the regulation of the transcription of cardiac genes. to confirm the significance for human heart failure, cardiac tissue specimens obtained from non-failing donor hearts and from explanted hearts from patien ... | 2002 | 12054667 |
| differences between the european carp (cyprinus carpio carpio) and the coloured carp (cyprinus carpio haematopterus) in susceptibility to thelohanellus nikolskii (myxosporea) infection. | thelohanellus nikolskii infection of the common carp (cyprinus carpio l.) has been a common parasitosis in the central european fish farms since the first detection of the parasite about 20 years ago. this parasite, introduced from the far east, causes intensive infection on the fins of fingerlings of the carp subspecies cultured in europe (european carp, cyprinus carpio carpio). this infection of the common carp occurs in the hungarian fish farms every year. until the present study, this parasi ... | 2002 | 12061236 |
| flavobacterium columnare (flexibacter columnaris) associated with severe gill necrosis in koi carp (cyprinus carpio l). | 2002 | 12074240 | |
| immunization of goldfish with excretory/secretory molecules of trypanosoma danilewskyi confers protection against infection. | trypanosoma danilewskyi is a protozoan that lives in blood and other tissues of fish. in the aquaculture industry, economic losses may be substantial, since the prevalence of infection may approach 100% and the parasite may cause significant mortality in farmed carp. most of the surviving fish acquire resistance after elimination of the primary infection. in this study, we examined whether protection against infection could be induced in naïve goldfish immunized with excretory-secretory (es) pro ... | 2002 | 12074929 |
| molecular variation of bothriocephalus acheilognathi yamaguti, 1934 (cestoda: pseudophyllidea) in different fish host species based on its rdna sequences. | the molecular variation in bothriocephalus acheilognathi yamaguti, 1934 from 11 species of freshwater fish collected in australia, china, the czech republic, england and hawaii was investigated by determining the nucleotide sequences of the internal transcribed spacer region. the length of the first and second internal transcribed spacer sequences of multiple individuals ranged from 553 to 571 bp and 553 to 615 bp, and the g + c content from 53.1 to 53.5%. the percentage sequence divergence vari ... | 2002 | 12075148 |
| high glucuronidation activity of environmental estrogens in the carp (cyprinus carpino) intestine. | many adverse effects on carp reproductive organs have been reported to be caused by exposure to environmental estrogens, such as nonylphenol and bisphenol a, which contaminate the aquatic environment. the glucuronidation activities of xenoestrogens (bisphenol a and diethylstilbestrol) and phytoestrogens (coumestrol, genistein and biochanin a), but not nonylphenol and octylphenol, were observed in microsomes prepared from carp organs. the highest levels of glucuronidation of environmental estroge ... | 2002 | 12084386 |
| molecular cloning of growth hormone encoding cdna of indian major carps by a modified rapid amplification of cdna ends strategy. | a modified rapid amplification of cdna ends (race) strategy has been developed for cloning highly conserved cdna sequences. using this modified method, the growth hormone (gh) encoding cdna sequences of labeo rohita, cirrhina mrigala and catla catla have been cloned, characterized and overexpressed in escherichia coli. these sequences show 96-98% homology to each other and are about 85% homologous to that of common carp. besides, an attempt has been made for the first time to describe a 3-d mode ... | 2002 | 12089475 |
| carp oil or oleic acid, but not linoleic acid or linolenic acid, inhibits tumor growth and metastasis in lewis lung carcinoma-bearing mice. | i examined the effects of carp oil, oleic acid, linoleic acid and linolenic acid on tumor growth and metastasis to the liver in mice implanted intrasplenically with highly metastatic lewis lung carcinoma (llc) tumors. carp oil (0.1 or 0.2 ml per mouse) significantly reduced tumor growth and metastasis to the liver. carp oil at 100 or 1000 mg/l inhibited the dna synthesis in llc cells, the capillary-like tube formation of human dermal microvascular endothelial cells (hmvec) at 1000 mg/l and the a ... | 2002 | 12097695 |
| a proposed serotyping system for flavobacterium psychrophilum. | to develop a common serological system for rapid and routine identification of the fish pathogen flavobacterium psychrophilum. | 2002 | 12100595 |
| association of a new c-cbl related protein with the very first stages of apoptosis induction. | this study investigates the involvement of the c-cbl proto-oncogene during the first stages of the apoptotic process. we have already shown that a c-cbl aptotosis-related protein of 90 kda (carp 90) is detected very rapidly in the cytoplasm as well as in the nucleus of murine thymocytes after hydrocortisone (hc) treatment. we report here that this protein appeared as well after in vivo treatment of mice by gamma-irradiation or injection of anti-cd3 monoclonal antibody, two potent thymic apoptosi ... | 2002 | 12102152 |
| temperature plasticity of contractile proteins in fish muscle. | three myosin heavy chain isoforms with different actin-activated mg(2+)-atpase activities were found in the fast skeletal muscle from carp (cyprinus carpio) acclimated to 10 and 30 degrees c. the composition of three types of myosin heavy chain was dependent on acclimation temperature, demonstrating the presence of temperature-specific myosin isoforms in carp. subsequently, the temperature-dependence of the sliding velocity of fluorescent f-actin in myosins isolated from 10 degrees c- and 30 deg ... | 2002 | 12110657 |
| modulation of gene expression in transgenic mouse hearts overexpressing calsequestrin. | calsequestrin (csq) is the major ca2+ binding protein of the cardiac sarcoplasmic reticulum (sr). transgenic mice overexpressing csq at the age of 7 weeks exhibit concentric cardiac hypertrophy, and by 13 weeks the condition progresses to dilated cardiomyopathy. the present study used a differential display analysis to identify genes whose expressions are modulated in the csq-overexpressing mouse hearts to provide information on the mechanism of transition from concentric cardiac hypertrophy to ... | 2002 | 12127059 |
| duckweed (lemna polyrhiza) leaf meal as a source of feedstuff in formulated diets for rohu (labeo rohita ham.) fingerlings after fermentation with a fish intestinal bacterium. | eight isonitrogenous (35% crude protein approximately) and isocaloric (4.2 kcal g(-1) approximately) diets were formulated including raw and fermented duckweed (lemna polyrhiza) leaf meal at 10%, 20%, 30% and 40% levels. a particular bacterial strain (bacillus sp.) isolated from carp (cyprinus carpio) intestine and having extracellular amylolytic, cellulolytic, proteolytic and lipolytic activities was used for leaf meal fermentation for 15 days at 37 degrees c. the fibre content of leaf meal red ... | 2002 | 12146637 |
| morphology and life cycle of apatemon hypseleotris species novum from australia including metacercariae viability and excystment. | experimental infection of pigeon squabs and rats with encysted metacercariae from the western carp gudgeon (hypseleotris klunzingeri) showed them to be infected with a new strigeid trematode, apatemon hypseleotris. growth and development of a. hypseleotris in pigeons were significantly higher than in rats. eggs appeared in pigeon faeces within 7-14 days; miracidia hatched within 15-21 days and in the snail lymnaea tomentosa released within 21 days. cercariae experimentally encysted in the leeche ... | 2002 | 12161969 |
| effects of a parasite-induced nephritis on osmoregulation in the common carp cyprinus carpio. | carp cyprinus carpio infected with the haemoflagellate trypanoplasma borreli undergo progressive nephritis associated with a destruction of approx. 40% of the nephric tubules. in an attempt to analyse the effect of the nephritis on the osmoregulation of affected carp, the clinical chemical properties of plasma and urine samples were analysed. parasitised carp excreted greater amounts of electrolytes in their urine than uninfected carp which excreted highly diluted urine with an osmolality of abo ... | 2002 | 12180703 |
| first record of neoergasilus japonicus (poecilostomatoida: ergasilidae), a parasitic copepod new to the laurentian great lakes. | the parasitic copepod neoergasilus japonicus, native to eastern asia, was first collected from 4 species of fish (fathead minnow, pimephales promelas; largemouth bass, micropterus salmoides; pumpkinseed sunfish, lepomis gibbosus; and yellow perch, perca flavescens) in july 1994 in saginaw bay, lake huron, michigan. further sampling in the bay in 2001 revealed infections on 7 additional species (bluegill, lepomis macrochirus; carp, cyprinus carpio; channel catfish, ictalurus punctatus; goldfish, ... | 2002 | 12197110 |
| immunisation of indian major carps against aeromonas hydrophila by intraperitoneal injection. | the indian major carps catla catla, labeo rohita and cirrhinus mrigala were immunised against the potent bacterial fish pathogen, aeromonas hydrophila by the intraperitoneal route, in field conditions. two different polyvalent antigen preparations namely, whole cell and extracellular products (ecp) were used. immunisation schedule consisting of a single booster dose given 28 days after priming resulted in a good agglutinating antibody response in the carps. kinetics of the response were similar ... | 2002 | 12201649 |
| a web-accessible complete transcriptome of normal human and dmd muscle. | we present an assessment of the complete transcriptome of human skeletal muscle in duchenne muscular dystrophy patient muscle and non-dystrophic controls (36 rnas analyzed from ten duchenne dystrophy and eight controls; approximately 65,000 gene/expressed sequence tag/probe sets queried on u95 five-genechip series and musclechip). the use of the multiple chip types allowed us to compare results from different probe sets for the same gene: we found excellent concordance between different probe se ... | 2002 | 12206807 |
| suppression in function of phagocytic cells in common carp cyprinus carpio l. injected with estradiol, progesterone or 11-ketotestosterone. | the modulation of phagocytic cells by beta-estradiol, 11-ketotestosterone, progesterone and cortisol was analysed in common carp (cyprinus carpio l.). carp were injected intraperitoneally (1 and 5 microg/kg fish) with each steroid and the activity of kidney macrophages was recorded 1 and 3 days post-injection. bovine serum albumin-injected fish served as controls. phagocytosis, superoxide anion and nitric oxide production were suppressed in all sex steroid treatments, and the suppression was in ... | 2002 | 12223196 |
| spring viremia of carp (svc). | spring viremia of carp (svc) is an important disease affecting cyprinids, mainly common carp cyprinus carpio. the disease is widespread in european carp culture, where it causes significant morbidity and mortality. designated a notifiable disease by the office international des epizooties, svc is caused by a rhabdovirus, spring viremia of carp virus (svcv). affected fish show destruction of tissues in the kidney, spleen and liver, leading to hemorrhage, loss of water-salt balance and impairment ... | 2002 | 12553453 |
| immunohistochemical study of the endocrine cells in the pancreas of the carp, cyprinus carpio (cyprinidae). | the regional distribution and relative frequency of some endocrine cells in the pancreas of the carp, cyprinus carpio linnaeus, belonging to the family cyprinidae in the order cypriniformes, were observed using specific mammalian antisera against insulin, glucagon, somatostatin and human pancreatic polypeptide (hpp) by peroxidase antiperoxidase (pap) method. the pancreas was divided into four regions (principal and secondary islets, exocrine and pancreatic duct regions). in addition, the pancrea ... | 2002 | 12819380 |
| occurrence of monogenea in italian freshwater fish (po river basin). | sixteen monogenean species were found on the skin and gills of 10 different fish species collected from north italian water bodies. cleidodiscus pricei from ictalurus melas; dactylogyrus alatus from alburnus alburnus; d. anchoratus, d. formosus and d. vastator from carassius carassius; d. difformis from scardinius erythrophthalmus; d. rutili from rutilus aula; d. tincae and gyrodactylus gasterostei from tinca tinca; d. ergensi from leuciscus cephalus and telestes muticellus; d. vistulae and para ... | 2002 | 12701382 |
| [genetic efficacy of low doses of ionizing radiation in chronically-irradiated small mammals]. | earlier we have established the genetic effects of low dose chronic irradiation in bank vole (somatic and germ cells, embryos), in pond carp (fertilized eggs, embryos, fry) and in laboratory mice (somatic and germ cells) in the range of doses from near-background to 10 cgy. these low dose effects observed in mammals and fish are not expected from extrapolation of high dose experiments. for understanding reasons this discrepancy the comparative analysis of genetic efficiency of low dose chronic i ... | 2002 | 12530144 |
| accumulation and loss of lindane in the muscles, liver and brain of some freshwater fish after intoxication with gamacarbatox suspension. | experiments were conducted under laboratory conditions on young specimens of carassius carassius l., cyprinus carpio l. and on adult specimens of leucaspius delineatus haeckel. the fish were subjected to a 30-day exposure to gamacarbatox suspension in a dose of 0.5 mg 1-1, containing 10% of lindane as active substance. directly after the experiment on day 30, and then 40 and 50, lindane content was assessed in the muscles, liver and brain using the method of gas chromatography with an electron c ... | 2002 | 12533964 |
| altered expression of cardiac ankyrin repeat protein and its homologue, ankyrin repeat protein with pest and proline-rich region, in atrophic muscles in amyotrophic lateral sclerosis. | cardiac ankyrin repeat protein, carp, is a protein that is restrictedly expressed in the heart but barely expressed in skeletal muscles. since carp is induced by pressure overload to the heart, it is proposed to be a genetic marker for cardiac hypertrophy. we recently identified a novel protein, ankyrin repeat protein with pest and proline-rich region (arpp), which is homologous to carp and is preferentially expressed in type 1 skeletal muscle fibers (cf. slow fibers). we also found that both ar ... | 2002 | 12679596 |
| cyclic amp-receptor proteins in human salivary glands. | in mammalian species, cyclic amp receptor proteins (carp) are the regulatory (r) subunits of cyclic amp-dependent protein kinase (pka), the cellular effector of cyclic amp-mediated signal transduction. an isoform of the pka type ii r subunit (rii), carp, is a polyfunctional protein, present in most tissues and cells. it is expressed in salivary and other glands of rodents, and secreted into the saliva of rats and man. the aim of the present study was to determine the expression of carp in human ... | 2002 | 14566599 |
| caryophyllidean tapeworms (platyhelminthes: eucestoda) from freshwater fishes in japan. | the following caryophyllidean tapeworms were found in freshwater fishes from japan (species reported from japan for the first time marked with an asterisk): family caryophyllaeidae: paracaryophyllaeus gotoi (motomura, 1927) from misgurnus anguillicaudatus (cantor); archigetes sieboldi leuckart, 1878 from pseudorasbora parva (temminck et schlegel) and sarcocheilichthys variegatus microoculus mori (new hosts); family lytocestidae: *caryophyllaeides ergensi scholz, 1990 from tribolodon hakuensis (g ... | 2001 | 11817451 |
| genetic manipulations in aquaculture: a review of stock improvement by classical and modern technologies. | the aim of this review was to highlight the extent to which the genetic technologies are implemented by the aquaculture industry. the review shows that some of the modern genetic technologies are already extensively applied by the diverse aquaculture industries, though not to the same extent for all important aquacultured species (according to fao 1998 figures). some species (common carp, atlantic salmon, rainbow trout, channel catfish, nile tilapia, and the pacific oyster) received concentrated ... | 2001 | 11841164 |
| isolation and characterisation of rhabdovirus from wild common bream abramis brama, roach rutilus rutilus, farmed brown trout salmo trutta and rainbow trout oncorhynchus mykiss in northern ireland. | rhabdovirus was isolated from wild common bream abramis brama during a disease outbreak with high mortality in northern ireland during may 1998. rhabdovirus was also isolated at the same time from healthy farmed rainbow oncorhynchus mykiss and brown trout salmo trutta on the same stretch of river and 11 mo later from healthy wild bream and roach rutilus rutilus in the same river system. experimental intra-peritoneal infection of bream and mirror carp cyprinus carpio var specularis with 2 of thes ... | 2001 | 11843142 |
| comparative metabolism of phenanthridine by carp (cyprinus carpio) and midge larvae (chironomus riparius). | abiotic transformation of azaarenes in the environment has been analysed extensively, but metabolism is less well described. to further elucidate preliminary observations of interspecific differences in azaarene metabolism by aquatic organisms, phenanthridine biotransformation by midge larvae and carp was studied. in both experiments, 6(5h)-phenanthridinone (phenanthridone) was found as an important metabolite. the fish were clearly capable of metabolising phenanthridine, but in the midge experi ... | 2001 | 11202649 |
| molecular cloning of the complement (c1r/c1s/masp2-like serine proteases from the common carp (cyprinus carpio). | the classical pathway of complement composed of c1, c4, and c2 is an antibody-dependent activation cascade that is present in jawed vertebrates. c1 is a ca2+-dependent complex of c1q, c1r, and c1s, and analogous to an initiation complex of the lectin pathway of complement, which consists of the mannose-binding lectin (mbl) homologous to c1q and the mbl-associated serine proteases (masps) homologous to c1r and c1s. thus divergence of clq and mbl and that of c1r, c1s and the masps are considered t ... | 2001 | 11220628 |
| monosaccharide uptake in common carp (cyprinus carpio) epc cells is mediated by a facilitative glucose carrier. | in most animal cells, transport of monosaccharides across the plasma membrane is mediated by glucose transporters (glut). mammals express at least five distinct transporters (gluts 1--5), which are well characterised both functionally and genetically. in contrast, the glucose transport system of fish remains poorly studied. here we report studies of hexose uptake in carp epc cells and cloning of a glucose transporter cdna from these cells. transport of radio-labelled methylglucose (3-omg) follow ... | 2001 | 11250543 |
| early expression of pituitary adenylate cyclase-activating polypeptide and activation of its receptor in chick neuroblasts. | to investigate the involvement of pituitary adenylate cyclase- activating polypeptide (pacap) and gh-releasing factor (grf) during early chick brain development, we established neuroblast- enriched primary cell cultures derived from embryonic day 3.5 chick brain. we measured increases in camp generated by several species-specific forms of the peptides. dose-dependent increases up to 5-fold of control values were measured in response to physiological concentrations of human/salmon, chicken, and t ... | 2001 | 11250943 |
| transforming growth factor-beta (tgf-beta) and vascular disease: carp as a putative tgf-beta target gene in the vessel wall. | 2001 | 11139465 | |
| transforming growth factor-beta/smads signaling induces transcription of the cell type-restricted ankyrin repeat protein carp gene through caga motif in vascular smooth muscle cells. | transforming growth factor (tgf)-beta plays a major role in the development of vascular diseases. despite the pleiotropic effects of tgf-ss on vascular smooth muscle cells (vsmcs), only a few genes have been characterized as direct targets of tgf-beta in vsmcs. cardiac ankyrin repeat protein (carp) has been thought to be expressed exclusively in the heart. in the present study, we showed that carp is expressed in the vasculature after balloon injury and in cultured vsmcs in response to tgf-beta. ... | 2001 | 11139470 |
| cloning, characterization and genomic structure of the natural killer cell enhancement factor (nkef)-like gene from homozygous clones of rainbow trout (oncorhynchus mykiss). | natural killer cell enhancement factor (nkef) belongs to the antioxidant protein family. in the human, nkef has the ability to enhance natural killer cell cytotoxic activity in vitro. in the present work, the cdnas of nkef from three strains of homozygous clones of rainbow trout were cloned from the splenic cdna library of one of the strains, osu142, and then by rt-pcr for the hot creek (hc) and arlee (ar) strains. the hc sequence has 99% sequence identity with both osu142 and ar. osu142 and ar ... | 2001 | 10980317 |
| acute and chronic influence of temperature on red blood cell anion exchange. | unidirectional (36)cl(-) efflux via the red blood cell anion exchanger was measured under cl(-) self-exchange conditions (i.e. no net flow of anions) in rainbow trout oncorhynchus mykiss and red-eared freshwater turtle trachemys scripta to examine the effects of acute temperature changes and acclimation temperature on this process. we also evaluated the possible adaptation of anion exchange to different temperature regimes by including our previously published data on other animals. an acute tem ... | 2001 | 11104709 |
| products of proteolytic cleavage of transferrin induce nitric oxide response of goldfish macrophages. | enzymatic cleavage product of transferrin induced the production of nitric oxide (no) by lps-stimulated goldfish macrophages. a no-inducing factor was purified from the supernatants of mitogen-stimulated goldfish kidney leukocytes using fast performance liquid chromatography (fplc) and the purified proteins analyzed by microcapillary reverse-phase hplc nano-electrospray tandem mass spectrometry. the proteins were identified as truncated forms of transferrin, having approximate molecular weights ... | 2001 | 11113281 |
| chlorinated hydrocarbons and biomarkers of exposure in wading birds and fish of the lower rio grande valley, texas. | during 1997 we evaluated reproductive success in colonial water birds nesting in the lower rio grande valley (lrgv), texas, and correlated success with concentrations of contaminants in eggs. we also measured steroid hormones and gonadosomatic index (gsi) as biomarkers of endocrine effects in common carp (cyprinus carpio). nest and fledging success of green herons (butorides virescens) and great egrets (ardea alba) were similar to those found in other parts of north america; however, nesting suc ... | 2001 | 11116345 |
| edwardsiella tarda mutants defective in siderophore production, motility, serum resistance and catalase activity. | edwardsiella tarda is a gram-negative bacterium that causes a systemic infection, edwardsiellosis, in fish. the virulence factors of this pathogen and its genetic determinants have not been systematically examined. in this study, tnphoa transposon mutagenesis was used to construct a library of 440 alkaline phosphatase (phoa(+)) fusion mutants from a total of 400000 transconjugants derived from ed. tarda ppd130/91. this library included genes for secreted and membrane-associated proteins normally ... | 2001 | 11158362 |
| purification of a novel myofibril-bound serine proteinase inhibitor (mbspi) from the skeletal muscle of lizard fish. | a novel myofibril-bound serine proteinase inhibitor (mbspi) was purified to homogeneity from the skeletal muscle of lizard fish (saurida wanieso). purification was carried out by ammonium sulfate fractionation, followed by column chromatographies on deae-sephacel, sp-sepharose and sephadex g-150. mbspi was purified 7.7-fold starting from the deae-sephacel fraction, with a yield of 0.2%. it is a monomeric protein with the molecular mass of 50 kda as estimated by sds-page and gel filtration. mbspi ... | 2001 | 11163300 |
| cadmium-binding to transferrin in the plasma of the common carp cyprinus carpio. | in this study, it was investigated by autoradiography with radioactive cadmium after western blotting of two-dimensional electrophoresis gels, to which proteins cadmium is mainly bound in plasma of common carp cyprinus carpio. the obtained results demonstrate that in carp plasma, cadmium is primarily bound to two high molecular weight proteins. relative small amounts are bound to a protein with m(r) approximately 60000. the other metal-binding protein, with m(r) approximately 70000 and pi approx ... | 2001 | 11166673 |
| reactive oxygen species uncouple external horizontal cells in the carp retina and glutathione couples them again. | we have investigated the effect of free radicals on the electrical gap junctions between horizontal cells in the carp retina. in our previous study, l-buthionine sulfoximine, an inhibitor of glutathione synthesis, caused uncoupling of horizontal cells four days after injection. in the present study, we have used paraquat, a generator of exogenous reactive oxygen species, to investigate whether it was the depletion of glutathione or an increase in the level of reactive oxygen species which result ... | 2001 | 11182257 |
| biochemical and morphological changes in carp (cyprinus carpio l.) liver following exposure to copper sulfate and tannic acid. | as a consequence of human activity various toxicants reach the aquatic ecosystems; humics may interact with them and may change their toxicity. many fish are exposed to a considerable concentration of humics and pollutants. because of paucity of data on the biochemical action of tannins in the presence of the fungicide cuso4 a comparative study was undertaken. the alterations of redox-parameters in carp liver were monitored and tissue necrosis was followed by measuring the plasma transaminase ac ... | 2001 | 11255117 |
| cytochrome p450 (p450) isoforms expression, p450 concentration, monooxygenase activities, reactive oxygen species formation, lipid peroxidation, and glutathione content in wild catch carp and tench liver--influence of a two weeks exposure to phenobarbital. | carps, both sexes, 3 years old, weighing about 1 kg, and tenches of both sexes, 6 years old, weight about 250 g, were caught from a thuringian lake without industrial pollution in november 1995 (fish without food uptake, water temperature at about 10 degrees c) and kept for 2 weeks in basins with clean water and addition of 0, 0.1, 1.0 or 10.0 mg/l phenobarbital-na (pb). the concentration of pb was controlled during and at the end of the exposure period. the animals were fed pellets, but no food ... | 2001 | 11256753 |
| mercury in sediment and fish from north mississippi lakes. | sediments and/or fish were collected from sardis, enid and grenada lakes, which are located in three different watersheds in north mississippi, in order to assess mercury contamination. the mean total mercury concentration in sediments from enid lake in 1997 was 0.154 mg hg/kg, while 1998 sediment concentrations in sardis, enid and grenada lakes were 0.112, 0.088 and 0.133 mg hg/kg, respectively. sediment mercury concentrations in 1999 were similar in all three lakes but, generally lower than 19 ... | 2001 | 11272915 |
| ammonia-induced cellular and immunological changes in juvenile cyprinus carpio infected with the blood fluke sanguinicola inermis. | immunological and structural changes in the thymus and pronephros of cyprinus carpio infected with the blood fluke, sanguinicola inermis for 30 days, and exposed to 0.5 mg nh4+/1 for 48 and 168 h were investigated. ultrastructural observations revealed cell disruption and highly vacuolated cytoplasm in the thymus. of the cells that remained intact there was a significant increase in thrombocytes after 48 h exposure to the pollutant. in addition, there was a decrease in lymphocytes following expo ... | 2001 | 11289070 |
| molecular cloning of preproinsulin cdnas from several osteoglossomorphs and a cyprinid. | several preproinsulin cdnas were isolated and characterized from four members of the osteoglossomorpha (an ancient teleost group); osteoglossum bicirrhosum (arawana), pantodon buchholzi (butterfly fish), notopterus chitala (feather fin knife fish), hiodon alosoides (goldeye) and gnathonemus petersii (elephantnose). in addition, we isolated and characterized the preproinsulin cdna from catostomus commersoni (white sucker, as a representative of a generalized teleost). the comparative analysis of ... | 2001 | 11306171 |
| myopalladin, a novel 145-kilodalton sarcomeric protein with multiple roles in z-disc and i-band protein assemblies. | we describe here a novel sarcomeric 145-kd protein, myopalladin, which tethers together the cooh-terminal src homology 3 domains of nebulin and nebulette with the ef hand motifs of alpha-actinin in vertebrate z-lines. myopalladin's nebulin/nebulette and alpha-actinin-binding sites are contained in two distinct regions within its cooh-terminal 90-kd domain. both sites are highly homologous with those found in palladin, a protein described recently required for actin cytoskeletal assembly (parast, ... | 2001 | 11309420 |
| seasonal population dynamics of neoechinorhynchus qinghaiensis in the carp, gymnocypris przewalskii przewalskii, from qinghai lake, china. | studies on the seasonal population dynamics of neoechinorhynchus qinghaiensis (acanthocephala: neoechinorhynchidae) in its fish host gymnocypris przewalskii przewalskii in the qinghai lake, china, were carried out with samples taken in may 1991, august 1992, november 1992 and february 1993. prevalences were higher than 44% in all seasons. the mean intensity of infection was above 124 worms per fish. the maximum intensity of worms recovered from a single fish was 1402 in the autumn of 1992. diffe ... | 2001 | 11316478 |
| histopathology of viremia-associated ana-aki-byo in combination with aeromonas hydrophila in color carp cyprinus carpio in japan. | a disease in which 'viremia-associated ana-aki-byo' is combined with an aeromonas hydrophila infection currently occurs and is highly transmissible in color carp cyprinus carpio in japan. in the present study, to determine the interrelation between a corona-like virus and a. hydrophila, we conducted transmission trials by cohabiting naturally diseased carp with healthy carp with skin that had been slightly damaged artificially. experimentally exposed fish successfully replicated the combination ... | 2001 | 11324811 |
| induction of specific cytotoxic t-cell activity against xenogeneic target cells in carp (cyprinus carpio). | to investigate the induction of cytotoxic t cells in carp (cyprinus carpio) after inoculation of fish with 2 xenogeneic line cells and to examine specificity of the cytotoxic activity. | 2001 | 11327471 |