Publications

[viral sexually transmitted diseases and their treatments].	in occidental countries, the sexually transmitted diseases (stds) originate mostly from virus: human papillomavirus (hpv) and herpes simplex virus (hsv). during the latest 15 to 20 years, their prevalence has been regularly growing. those viruses have in common a capacity for latency. the persistence of the virus in keratinocytes (pvh) or in ganglionic cells (hsv) and the lack of an efficient preventive or final curative treatment have several consequences: 1) a possible capacity of transmitting ...	1998	9805942
solitary pulmonary papillomas in adults: a clinicopathologic and in situ hybridization study of 14 cases combined with 27 cases in the literature.	solitary endobronchial papillomas in adults are rare neoplasms. only sporadic cases have been documented. the histologic classification of these tumors remains problematic, and little is known about their clinical behavior. the clinical and pathologic features of 13 endobronchial papillomas and a single endobronchiolar papilloma were reviewed. in situ hybridization for human papillomavirus (hpv) types 6/11, 16/18, and 31/33/51 was performed on seven cases. twenty-seven additional well-documented ...	1998	9808125
in vitro construction of pseudovirions of human papillomavirus type 16: incorporation of plasmid dna into reassembled l1/l2 capsids.	lack of permissive and productive cell cultures for the human papillomaviruses (hpvs) has hindered the study of virus-neutralizing antibodies and infection. we developed a cell-free system generating infectious hpv16 pseudovirions. hpv16 l1/l2 capsids, which had been self-assembled in insect cells (sf9) expressing virion proteins l1 and l2, were disassembled with 2-mercaptoethanol (2-me), a reducing agent, and reassembled by removal of 2-me in the presence of a beta-galactosidase expression plas ...	1998	9811779
genital human papillomavirus infection in women who have sex with women.	genital infection with human papillomavirus (hpv), as determined by polymerase chain reaction detection of hpv dna and prevalence of hpv-6 and -16 serum antibodies, was investigated in 149 women who were sexually active with women. by use of hpv l1 consensus primers and hybridization to types 6/11, 16, 18, 31/33/35/39, and 45 and a generic probe, hpv dna was detected in 30% of subjects; of these, 20% had type 31/33/35/39, 18% had type 16, and 2% had type 6/11. of 21 subjects reporting no prior s ...	1998	9815211
expression of human papillomavirus 6 in inverted papilloma arising in a renal transplant recipient.	a 36-year-old renal transplant recipient taking cyclosporin a presented with bilateral nasal polypoid lesions involving the nasal septum and lateral nasal walls. pathologic findings from surgical excision demonstrated inverted papilloma (ip) with focal atypia and mild dysplasia. dna extracted from the tissue was tested with the polymerase chain reaction (pcr) using human papillomavirus (hpv) e6 and l1 consensus primers. this revealed amplification of the expected size fragment consistent with th ...	1998	9432079
inhibition of hpv-16 e6/e7 immortalization of normal keratinocytes by hairpin ribozymes.	hpv-16 e6 and e7 genes are required to efficiently immortalize a broad spectrum of cell types including cervical keratinocytes. therefore, the e6/e7 genes can be considered relevant targets for anti-cancer therapy. we produced several engineered hairpin (hp) ribozymes to specifically disrupt hpv-16 e6/e7 mrna. after extensive biochemical characterization, one anti-e6 hp ribozyme (r434) was selected for in vivo testing because of its superior catalytic capabilities. when expressed in cis, r434 ef ...	1998	9448307
recent advances in thin-layer cytology.	in recent years liquid-based cytology has emerged as an alternative to conventional cytopreparatory methods. in particular, the thinprep system has found broad acceptance in non-gynecologic cytopreparation. many laboratories have successfully applied this technique to body fluids (e.g. urine, pleural effusions), brushing samples (e.g. gastrointestinal tract, lung) and fine-needle aspiration. most comparative studies have shown the thinprep system to perform as well as or better than conventional ...	1998	9451555
human papillomavirus types 52 and 58.		1998	9455813
a distamycin a-inducible fragile site, fra8e, located in the region of the hereditary multiple exostoses gene, is not involved in hpv16 dna integration and amplification.	the rare fragile site is a specific point on a chromosome that is expressed as an isochromatid gap or break under certain conditions of cell culture and is inherited in a mendelian codominant fashion. five folate-sensitive fragile sites were cloned, and the molecular basis of fragile site mutation was shown to be a new class of mutation, called dynamic mutation, resulting from an allelic expansion of (ccg)n repeats. the mechanism responsible for other types of rare fragile sites, i.e., distamyci ...	1998	9460496
clinical and molecular pathology of aggressive schneiderian papilloma involving the temporal bone.	inverting papilloma is a benign but locally aggressive sinonasal tumor that rarely involves the middle ear or temporal bone.	1998	9464958
antibodies against early proteins of human papillomaviruses as diagnostic markers for invasive cervical cancer.	cervical cancer is the most prevalent tumor in developing countries and the second most frequent cancer among females worldwide. specific human papillomaviruses (hpvs) and, most notably, hpv types 16 and 18 are recognized as being causally associated with this malignancy. antibodies against early hpv proteins e6 and e7 have been found more often in patients with tumors than in controls. existing peptide enzyme-linked immunosorbent assays (elisas) for the detection of anti-e6 and anti-e7 antibodi ...	1998	9466762
retinoblastoma protein recruits histone deacetylase to repress transcription.	the retinoblastoma protein (rb) silences specific genes that are active in the s phase of the cell cycle and which are regulated by e2f transcription factors. rb binds to the activation domain of e2f and then actively represses the promoter by a mechanism that is poorly understood. here we show that rb associates with a histone deacetylase, hdac1, through the rb 'pocket' domain. association with the deacetylase is reduced by naturally occurring mutations in the pocket and by binding of the human ...	1998	9468139
down-regulation of human sialyltransferase gene expression during in vitro human keratinocyte cell line differentiation.	sialic acids play important roles in biological processes, such as cell-cell communication and cell-matrix interaction. histochemical analysis using pna and lfa lectin has shown that the expression of alpha 2,3-sialic acid linked to gal beta 1,3galnac is high in basal cells and decreases following further keratinocyte differentiation. in the present study, we used an in vitro keratinocyte cell line differentiation model to study expression of alpha 2,3-sialic acid linked to gal beta 1,3 galnac. ...	1998	9473501
squamous intraepithelial lesions in cervical smears of human immunodeficiency virus-seropositive adolescents.	various authors have reported a high rate of human papillomavirus (hpv) infection and hpv-related neoplasias in human immunodeficiency virus (hiv)-seropositive women. on the other hand, young women are most susceptible to cervical infection because of immaturity of the cervix, as it appears that hpv has more access to the basal cells of the differentiating epithelium. the purpose of the present work was to study cervical smears of 82 adolescent hiv-seropositive women (13-21 years of age) to sear ...	1998	9484635
human fibroblasts expressing the human papillomavirus e6 gene are deficient in global genomic nucleotide excision repair and sensitive to ultraviolet irradiation.	we investigated the role of wild-type p53 activity in modulating nucleotide excision repair after uv irradiation in normal and p53-deficient primary human fibroblasts created by expression of the human papillomavirus 16 e6 gene. compared with parental cells, the e6-expressing fibroblasts were deficient in global genomic repair of both uv-induced cyclobutane pyrimidine dimers and 6-4 photoproducts but exhibited normal transcription-coupled repair. the e6-expressing cells were also more sensitive ...	1998	9485006
novel mechanisms of e2f induction by bk virus large-t antigen: requirement of both the prb-binding and the j domains.	e2f activity is regulated in part by the retinoblastoma family of tumor suppressor proteins. viral oncoproteins, such as simian virus 40 (sv40) large-t antigen (tag), adenovirus e1a, and human papillomavirus e7, can disrupt the regulation of cellular proliferation by binding to prb family members and dissociating e2f-prb family protein complexes. bk virus (bkv), which infects a large percentage of the human population and has been associated with a variety of human tumors, encodes a tag homologo ...	1998	9488491
trophoblasts are the preferential target for human papilloma virus infection in spontaneously aborted products of conception.	in a recent study it has been shown that human papillomavirus (hpv) infection was threefold more prevalent in spontaneous abortion specimens compared with elective specimens (60% [15 of 25] v 20% [3 of 15], respectively) as analyzed by broad-spectrum hpv polymerase chain reaction (pcr) amplification and dot-blot hybridization with an hpv-16 probe. in this study, archival paraffin-embedded tissue from a subset of previously analyzed spontaneous abortion cases were reanalyzed by in situ pcr amplif ...	1998	9490277
identification of single amino acids in the human papillomavirus 11 e2 protein critical for the transactivation or replication functions.	the papillomavirus e2 protein is required for viral transcriptional regulation and replication. the e2 protein has a modular structure with two highly conserved domains, a sequence-specific dna-binding and dimerization domain and a conserved n-terminus which is important for transcriptional transactivation, replication, and interaction with the e1 protein to determine which specific amino acids or regions in the n-terminus were important for the replication or transactivation functions. single a ...	1998	9499806
dna structure and flexibility in the sequence-specific binding of papillomavirus e2 proteins.	the papillomavirus e2 proteins are transcriptional regulators that bind to a consensus dna sequence accg nnnn cggt. multiple copies of this binding site are found in the viral genomes. the affinities of the naturally occurring binding sites for the e2 proteins are predominantly dependent upon the sequence of the nnnn spacer. the hierarchies of binding site affinities among the sites present in the viral genomes result in differential occupancy during the viral life-cycle. in turn, this different ...	1998	9500925
detection of human papillomavirus type 35 in a nodular cutaneous tumor in a patient infected with human immunodeficiency virus.		1998	9502486
plasma concentrations of micronutrients during a nine-month clinical trial of beta-carotene in women with precursor cervical cancer lesions.	the effects of oral supplementation of a 30-mg dose of beta-carotene on the plasma levels of carotenoids, tocopherols, and retinol were studied sequentially in 69 patients participating in a nine-month randomized placebo controlled trial conducted to examine efficacy of beta-carotene to induce regression of cervical intraepithelial neoplasia. at each visit (baseline and 1.5, 3, 6, 9, 10.5, and 15 mo), blood samples were collected and the levels of six micronutrients were determined by high-perfo ...	1998	9507512
hpv 16 e7 antibody levels in cervical cancer patients: before and after treatment.	antibody response to hpv16 e7 oncoprotein may represent a marker of cervical cancer. a hpv16 gst-e7 fusion protein was used in a western blot assay to analyse the hpv16 e7 antibody response in 30 patients before and after treatment for cervical carcinoma (stage iib or iiib). patients were treated with three courses of cisplatin/bleomycin therapy followed by surgery, or with surgery alone. thirteen out of 30 patients had serum antibodies to hpv16 e7 antigen. three months after chemotherapy little ...	1998	9515768
interphase cytogenetic evidence for distinct genetic pathways in the development of squamous neoplasia of the uterine cervix.	human papillomavirus (hpv) infection has been implicated as an etiologic factor in most cervical cancers. however, additional genetic alterations are thought to be required for the development of a carcinogenic genotype. in the present study, interphase cytogenetics utilizing pericentromeric probes specific for chromosomes 1, 3, 11, 17, 18, and x was performed on paraffin-embedded tissue sections from 25 high-grade squamous intraepithelial lesions (sils) and 25 invasive squamous cell carcinomas ...	1998	9520942
detection of human papillomavirus in archival tissues. comparison of in situ hybridization and polymerase chain reaction.	formalin-fixed, paraffin-embedded tissues in pathology archives are an important resource for molecular epidemiology studies. use of these tissues requires that assays be optimized to account for inevitable variations in tissue fixation and processing that occur in the performance of routine histology. we compared results of colorimetric in situ hybridization (ish) to l1 consensus polymerase chain reaction (pcr) for detection and typing of human papillomavirus (hpv) in 180 blocks of archival tis ...	1998	9524200
virologic, immunologic, and clinical parameters in the incidence and progression of anal squamous intraepithelial lesions in hiv-positive and hiv-negative homosexual men.	anal cancer may be preceded by anal squamous intraepithelial lesions (asil), but the natural history of asil is poorly understood. in this report, we characterize the 2-year incidence and progression of low-grade sil (lsil) and high-grade sil (hsil) in a cohort study in 346 hiv-positive and 262 hiv-negative homosexual or bisexual men. subjects were studied at defined intervals using anal cytology, anoscopy with biopsy of visible lesions, human papillomavirus (hpv) testing, hiv serostatus, cd4 le ...	1998	9525431
the carboxyl-terminal region of the human papillomavirus type 16 e1 protein determines e2 protein specificity during dna replication.	the mechanism of dna replication is conserved among papillomaviruses. the virus-encoded e1 and e2 proteins collaborate to target the origin and recruit host dna replication proteins. expression vectors of e1 and e2 proteins support homologous and heterologous papillomaviral origin replication in transiently transfected cells. viral proteins from different genotypes can also collaborate, albeit with different efficiencies, indicating a certain degree of specificity in e1-e2 interactions. we repor ...	1998	9525677
vaginal immunization with recombinant gram-positive bacteria.	many viral and bacterial pathogens enter the body through the genital mucosa. therefore, one of the major goals of a vaccine against sexually transmitted diseases (stds) should be to induce an immune response in the genital mucosa capable of controlling the entry of the pathogen. our approach for the development of vaccines against stds is based on the use of nonpathogenic gram-positive bacteria as live vaccine vectors.	1998	9526609
an increased risk of cervical intra-epithelial neoplasia grade ii-iii among human papillomavirus positive patients with the hla-dqa10102-dqb10602 haplotype: a population-based case-control study of norwegian women.	several recent studies have reported different associations between hla specificities and human papillomavirus (hpv)-associated disease of the cervix. we report the distribution of dqa1 and dqb1 genes and hpv infection in a population-based case-control study including 92 patients with histologically verified cervical intraepithelial neoplasia grade ii-iii (cin ii-iii) (thus including moderate and severe dysplasia and carcinoma in situ) and 225 control subjects. we found an overrepresentation of ...	1998	9533756
characterization of a partially folded monomer of the dna-binding domain of human papillomavirus e2 protein obtained at high pressure.	the pressure-induced dissociation of the dimeric dna binding domain of the e2 protein of human papillomavirus (e2-dbd) is a reversible process with a kd of 5.6 x 10(-8) m at ph 5.5. the complete exposure of the intersubunit tryptophans to water, together with the concentration dependence of the pressure effect, is indicative of dissociation. dissociation is accompanied by a decrease in volume of 76 ml/mol, which corresponds to an estimated increase in solvent-exposed area of 2775 a2. there is a ...	1998	9535893
analysis of fhit transcripts in cervical and endometrial cancers.	carcinoma of the uterine cervix is a common malignancy, and many affected women, have been found to exhibit loss of heterozygosity (loh) in the chromosome 3p region. recent studies have localized the fhit (fragile histidine triad) gene in this region and also demonstrated a high frequency of abnormalities of this gene in various cancers. to determine the role of the fhit gene in cervical and uterine carcinomas, 16 cases of cervical carcinoma and 7 cases of endometrial carcinoma, as well as nearb ...	1998	9537583
human papillomavirus type 16 and risk of preinvasive and invasive vulvar cancer: results from a seroepidemiological case-control study.		1998	9540960
high incidence of anal high-grade squamous intra-epithelial lesions among hiv-positive and hiv-negative homosexual and bisexual men.	the incidence of anal cancer among homosexual men exceeds that of cervical cancer in women, and hiv-positive homosexual men may be at even higher risk than hiv-negative men. cervical cancer is preceded by high-grade squamous intra-epithelial lesions (hsil) and anal hsil may similarly be the precursor to anal cancer. in this study, we describe the incidence of and risk factors for hsil in hiv-positive and hiv-negative homosexual and bisexual men.	1998	9543448
papillomavirus-induced genital warts in a girl--management by surgery and immunomodulating therapy.	a 4-year-old girl with condylomata acuminata of the vulva and papular warts of the surrounding skin is presented. the lesions were removed by surgery. histologic investigation showed koilocytosis of the squamous epithelium and in-situ hybridization revealed human papilloma virus type 6 infection. there were no signs of sexual abuse or sexual transmission of the virus. after ablation, an interferon-containing ointment was applied. in order to prevent recurrence, a low-molecular-weight immunomodul ...	1998	9553198
disruption of the e1 and e2 reading frames of hpv 16 in cervical carcinoma is associated with poor prognosis.	the e1 and e2 reading frames of 158 cervical carcinoma samples containing human papillomavirus (hpv) 16 were mapped using polymerase chain reaction (pcr). the reading frames were amplified using primers spanning the entire genes. of the analyzed samples, 23% showed no amplification with the e1 primers and 29% showed no amplification with the e2 primers. there was an overlap, but not complete identity, between the e1- and e2-disrupted groups. all e1- and e2-negative samples were further analyzed ...	1998	9553812
analysis of human papillomavirus infection and molecular alterations in adenocarcinoma of the cervix.	although molecular alterations involved in the development of squamous cell carcinoma of the cervix have been extensively described, these genetic changes have not been as well characterized in the development of cervical adenocarcinoma. twenty-seven paraffin-embedded adenocarcinomas of the cervix, including three cases of adenoma malignum, were analyzed for molecular alterations associated with other gynecologic malignancies. the presence of human papillomavirus (hpv) was assessed by polymerase ...	1998	9556417
nuclear matrix attachment regions of human papillomavirus type 16 point toward conservation of these genomic elements in all genital papillomaviruses.	the gene functions, transcriptional regulation, and genome replication of human papillomaviruses (hpvs) have been extensively studied. thus far, however, there has been little research on the organization of hpv genomes in the nuclei of infected cells. as a first step to understand how chromatin and suprachromatin structures may modulate the life cycles of these viruses, we have identified and mapped interactions of hpv dnas with the nuclear matrix. the endogenous genomes of hpv type 16 (hpv-16) ...	1998	9557642
imiquimod, a patient-applied immune-response modifier for treatment of external genital warts.	genital human papillomavirus infection is one of the most common sexually transmitted diseases. imiquimod is a new agent, an immune-response modifier, that has been demonstrated to have potent in vivo antiviral and antitumor effects in animal models. the present prospective, multicenter, double-blind, randomized, vehicle-controlled trial evaluated the efficacy and safety of daily patient-applied imiquimod for up to 16 weeks for the treatment of external genital warts. wart recurrence was investi ...	1998	9559784
presence of human papilloma virus dna in pelvic lymph nodes can predict unexpected recurrence of cervical cancer in patients with histologically negative lymph nodes.	patients without any evidence of lymph node metastases are supposed to have a fair prognosis, but some of these patients develop recurrent disease unexpectedly after surgery. the object of this study is to examine whether the detection of human papilloma virus (hpv) dna could be used as a diagnostic marker to predict such recurrences. two hundred and thirty-six patients undergoing radical hysterectomy and pelvic lymphadenectomy for stage ib and ii cervical cancer at okayama university hospital ( ...	1998	9563893
[perspectives for the development of vaccines and immunotherapy against cervico-uterine cancer].	cervical cancer represents a severe public health problem and has been associated to the presence of human papillomavirus. strategies are presently being tested which target the virus to attempt to control disease progress. studies on the humoral and cell-mediated immunity of the papillomavirus infection have been useful in the development of a vaccine. synthetic virus-like particles have been validated as vaccine against several animal papillomaviruses and used to map the seroepidemiology of th ...	1998	9567657
type specific and genotype cross reactive b epitopes of the l1 protein of hpv16 defined by a panel of monoclonal antibodies.	mouse monoclonal antibodies (mabs) were raised against the major capsid protein, l1, of human papillomavirus type 16 (hpv16), produced in escherichia coli with the expression plasmid ptrcl1. epitope specificity could be assigned to 11 of these 12 antibodies using a series of linear peptides and fusion proteins from hpv16. one mab (mc53) recognized a novel linear epitope that appears to be unique to the hpv16 genotype. a further 11 mabs were characterized as recognizing novel and previously defin ...	1998	9568027
human papillomavirus and head and neck cancer: epidemiology and molecular biology.	human papillomaviruses (hpv) are known to cause cancers of the cervix and other anogenital tract sites. molecular biology has provided some evidence as to the specific mechanisms involved in the hpv-related carcinogenesis. epidemiologic and molecular biology studies have also suggested that hpv infection may be associated with cancers of the head and neck.	1998	9570632
prevalence, risk factors, and accuracy of cytologic screening for cervical intraepithelial neoplasia in women with the human immunodeficiency virus.	the objective was to evaluate the sensitivity and specificity of cervical cytology in women infected with the human immunodeficiency virus (hiv), risk factors for abnormal cytology in hiv-infected and uninfected women, and risk factors for histologic diagnosis of cervical intraepithelial neoplasia (cin) in hiv-infected women.	1998	9570972
molecular analysis of h2o2-induced senescent-like growth arrest in normal human fibroblasts: p53 and rb control g1 arrest but not cell replication.	human diploid fibroblasts lose the capacity to proliferate and enter a state termed replicative senescence after a finite number of cell divisions in culture. when treated with sub-lethal concentrations of h2o2, pre-senescent human fibroblasts enter long-term growth arrest resembling replicative senescence. to understand the molecular basis for the h2o2-induced growth arrest, we determined the cell cycle distribution, levels of p53 tumour suppressor and p21 cyclin-dependent kinase inhibitor prot ...	1998	9576849
dna-eia to detect high and low risk hpv genotypes in cervical lesions with e6/e7 primer mediated multiplex pcr.	oncogenicity of human papillomavirus (hpv) dna in premalignant and malignant uterine cervical diseases is mainly induced by e6/e7 open reading frame (orf). the presence of an oncogenic hpv dna may be a diagnostic marker for the detection of cytologically negative smears.	1998	9577370
human papillomaviruses are commonly found in normal skin of immunocompetent hosts.	we have previously demonstrated, by the combined application of two degenerate polymerase chain reaction primer sets, the presence of human papillomavirus (hpv) dna in 91% of cutaneous squamous cell cancers from renal allograft recipients, with multiple types being present in one-third of these tumors. five hpv types--hpv 20, hpv 23, hpv 38, dl40, and dl267--accounted for 73% of positive results. these hpv types are all related to the epidermodysplasia verruciformis group, and hpv 38 was origina ...	1998	9579540
trends in seroprevalence of human papillomavirus type 16 among pregnant women in stockholm, sweden, during 1969-1989.	to assess long-term trends in the prevalence of oncogenic human papillomavirus (hpv) infection, we performed a cross-sectional serosurvey of the seroprevalence of the major oncogenic hpv type, hpv16, among 3,512 pregnant women undergoing population-based serological screening at the first trimester of pregnancy in the same catchment area in stockholm, sweden, during 1969, 1983 or 1989. the overall hpv16 seroprevalence rates were 16% in 1969, 22% in 1983 and 21% in 1989. seroprevalence was signif ...	1998	9579570
intracellular expression of a single-chain antibody directed against human papillomavirus type 16 e7 oncoprotein achieves targeted antineoplastic effects.	human papillomavirus type 16 (hpv16) e7 is a viral oncoprotein that is believed to play a major role in cervical neoplasia. anti-hpv16 e7 intracellular single-chain antibodies (scfvs) were constructed to down-regulate hpv16 e7 oncoprotein in hpv dna-containing cell lines. in these studies, we transfected anti-e7 scfvs into the hpv16-positive human cervical carcinoma cell lines caski and siha and tested them for their ability to inhibit cell proliferation and alter the level of hpv16 e7 oncoprote ...	1998	9581830
p53-dependent signaling sustains dna replication and enhances clonogenic survival in 254 nm ultraviolet-irradiated human fibroblasts.	the cyclin-dependent kinase inhibitor p21(waf1/cip1/sdi1/cap20) exists in normal human fibroblasts in a quaternary complex with a cyclin, a cyclin-dependent kinase, and proliferating cell nuclear antigen. a model was proposed in which, during p53-mediated suppression of cell proliferation following treatment with 254 nm uv radiation (uvc), the enhanced expression of p21 might inhibit dna replication by virtue of its interactions with proliferating cell nuclear antigen. to test this model, we exa ...	1998	9581844
papillomavirus and anal carcinoma.	human papilloma virus (hpv; 16 and 18) is known to play an important etiologic role in cervical dysplasia, but its relationship with anal carcinoma is still unclear. surgical samples from 80 female patients treated for anal epidermoid carcinoma in the polyclinic of surgery in geneva between 1976 and 1989 were retrospectively studied. of these, hpv detection was performed in 41 whose dna was preserved well enough to allow such an analysis. seventeen (42%) samples contained hpv, with a high percen ...	1998	9638498
the clinical value of digene hybrid capture hpv dna testing in a referral-based population with abnormal pap smears.	the hybrid capture human papillomavirus (hpv) dna assay is offered by the manufacturer to assist clinicians with patients with ascus pap smear results to assess the risk factor and to potentially direct follow-up of these patients. in our practice, a gynecologic oncology practice that has a referral based population with abnormal pap smears, our purpose was to evaluate the patients referred with all grades of abnormal cervical cytology.	1998	9641214
screening of potential semen donors for sexual transmitted diseases.	the risk of infection with sexually transmitted diseases (stds) is of great concern to couples undergoing therapeutic donor insemination. goal of study: we sought to determine the prevalence of stds in potential semen donors and assess the rate of acquisition of new infection during the follow-up period.	1998	9643155
human papilloma virus infection in the oral cavity--a source of infection.		1998	9643338
viruses in human oral cancers.	the role of viruses in the etiology of human oral cancer is critically reviewed. available evidences show a positive correlation for human oral cancer with human papilloma virus (hpv), herpes simplex virus (hsv) and human herpes virus type-6 (hhv-6), having strongest association with hpv. these viruses may act alone or in combination with other carcinogens in the genesis of head and neck malignancies.	1998	9646230
the status of human papillomavirus and tumor suppressor genes p53 and p16 in carcinomas of uterine cervix from india.	infection with the high-risk strain of human papillomaviruses (hpvs) and the inactivation of the tumor suppressor gene p53 through mutation are important factors in cervical carcinogenesis. to know whether such events would occur in cervical carcinomas of indians, 43 tumors (consisting of 36 of stage iii b and 6 of stage ii b) were screened for p53 and p16 gene mutations.	1998	9648588
basal and human papillomavirus e6 oncoprotein-induced degradation of myc proteins by the ubiquitin pathway.	we have previously shown that the degradation of c-myc and n-myc in vitro is mediated by the ubiquitin system. however, the role of the system in targeting the myc proteins in vivo and the identity of the conjugating enzymes and possible ancillary proteins involved has remained obscure. here we report that the degradation of the myc proteins in cells is inhibited by lactacystin and mg132, two inhibitors of the 20s proteasome. inhibition is accompanied by accumulation of myc-ubiquitin conjugates. ...	1998	9653139
a c-rasha mutation in the metastasis of a human papillomavirus (hpv)-18 positive penile squamous cell carcinoma suggests a cooperative effect between hpv-18 and c-rasha activation in malignant progression.	human papillomaviruses (hpv) have been implicated in the etiology of anogenital squamous epithelial tumors. of the 65 hpv strains, subtypes hpv-16 and hpv-18 frequently are associated with malignant conditions and are capable of transforming keratinocytes in vitro. however, additional cellular changes are necessary to confer tumorigenicity to hpv-infected cells. secondary events implicated in the progression to malignancy include loss of tumor suppressor genes such as p53 and/or activation of ce ...	1998	9655301
synthetic peptides of human papillomavirus type 18 e6 harboring hla-a2.1 motif can induce peptide-specific cytotoxic t-cells from peripheral blood mononuclear cells of healthy donors.	to identify cytotoxic t-cell (ctl) epitopes against human papillomavirus type 18 (hpv 18) e6 protein that might be useful for developing peptide-based vaccine against hpv 18 infection, 18 peptides which possibly contain ctl epitopes were selected on the basis of previously described human leukocyte antigen (hla)-a2.1-binding motif and chemically synthesized. in the binding assay of the synthetic peptides, 8 out of 18 synthetic peptides enhanced the expression of hla-a2.1 molecules on t2 cell sur ...	1998	9660068
mutations in the tp53 gene and protein expression of p53, mdm 2 and p21/waf-1 in primary cervical carcinomas with no or low human papillomavirus load.	several studies have focused on the role of p53 inactivation in cervical cancer, either by inactivating mutations in the tp53 gene or by degradation of the p53 protein by human papillomavirus (hpv). in this study, primary cervical carcinomas from 365 patients were analysed for presence of hpv using both consensus primer-sets and type-specific primer-sets. nineteen samples were determined to have no or low virus load, and were selected for further analyses: mutation screening of the tp53 gene usi ...	1998	9662253
risk factors for incident and recurrent condylomata acuminata among men. a population-based study.	the rapid increase in the number of physician office visits for condylomata acuminata and the association of human papillomavirus and cancer has prompted renewed interest in the epidemiology of this sexually-transmitted disease. few epidemiologic studies have examined what risk factors are associated with condylomata acuminata in men.	1998	9662760
detection of human papillomavirus dna in puva-associated non-melanoma skin cancers.	psoralen and uva (puva) photochemotherapy is associated with a dose-dependent increased risk of nonmelanoma skin cancer in patients treated for psoriasis. like ultraviolet b radiation, puva is both mutagenic and immunosuppressive and may thus act as a complete carcinogen; however, the reversed squamous to basal cell carcinoma ratio (scc:bcc) in puva-treated patients, also seen in immunosuppressed renal transplant recipients, suggests a possible cofactor role for human papillomavirus (hpv) infect ...	1998	9665398
detection of low copy numbers of hpv dna by fluorescent in situ hybridization combined with confocal microscopy as an alternative to in situ polymerase chain reaction.	in genital lesions infected by human papillomavirus (hpv), histological criteria and hpv dna typing are of prognostic value. therefore, non-radioactive methods such as in situ hybridization are used extensively since they preserve the histological organization of the tissue, and allow the detection and characterization of hpv dna. however, the sensitivity of these methods is often limited to detection of low copy numbers of hpv dna in isolated cells or in tissue sections, and therefore alternati ...	1998	9672129
clinicopathologic study of basaloid squamous carcinoma of the upper aerodigestive tract.	the clinicopathologic and immunohistochemical characteristics of nine cases of basaloid squamous carcinoma (bsc) of the upper aerodigestive tract are reported, along with the results of an in situ hybridization for human papilloma virus (hpv) dna. the cases were selected through a review of 237 head and neck carcinomas, and were located in the supraglottic larynx (5), hypopharynx (2), and the base of tongue (2). the patients were 7 males and 2 females with the mean age of 62. bscs were histologi ...	1998	9681804
establishment of ameloblastoma cell line, am-1.	ameloblastomas are slowly growing, locally invasive neoplasms with a potentially destructive behaviour. the molecular mechanisms that regulate the cell growth and invasion of ameloblastoma cells are unknown. because ameloblastoma cells placed in culture have a very limited lifespan, the establishment of immortalized clones of ameloblastoma cells would aid its study. we produced an immortalized ameloblastoma cell line (am-1) using human papillomavirus type-16. this cell line maintains epithelial ...	1998	9682983
genital infections with human papillomavirus (hpv).		1998	9686734
human papillomavirus type 16 is detected in transitional cell carcinomas and squamotransitional cell carcinomas of the cervix and endometrium.	the etiologic role of human papillomavirus (hpv) in a variety of squamous neoplasms, including malignant and premalignant lesions of the cervix, is well established. the presence of hpv, predominantly hpv types 16 and 18, in adenocarcinomas of the endometrium has also been reported, although less commonly. although rare, transitional cell carcinoma (tcc) in the female genital tract, including such sites as the cervix, endometrium, and ovary, has been described. hpv, however, has not been previou ...	1998	9690545
risk factors for cervical neoplasia in denmark.	with the overall goal of elucidating the risk factor pattern for cervical neoplasia, two case-control studies and a prospective cohort study were conducted. the first case-control study focused on female lifestyle risk factors. it was designed to include all women (aged 20-49 years) in greater copenhagen, diagnosed with invasive cervical cancer or carcinoma in situ (cis) from january 1985 to december 1986. they were identified from the danish cancer registry. an age-stratified control group was ...	1998	9693662
hla class i phenotype and genotype alterations in cervical carcinomas and derivative cell lines.	downregulation of hla class i expression is a common event in tumor biology. various underlying mechanisms have been defined in different tumors, but the knowledge of hla loss mechanisms in cervical carcinoma is limited. to identify causalities for loss of surface expression, we performed a detailed investigation of hla class i phenotypes and genotypes in 5 primary cervical tumors and on derivative cell lines. protein expression on primary tissues and cell lines was evaluated by immunohistochemi ...	1998	9694355
hpv prevalence among mexican women with neoplastic and normal cervixes.	genital human papillomavirus (hpv) infection is causally linked to cervical cancer, yet little is known regarding hpv prevalence in cancerous and normal women in mexico, a country with a high cervical cancer incidence. we studied 185 mexican women among the patients attending gynecological outpatient clinics in four hospitals in mexico city. each woman had a pap smear, a colposcopy, and, when necessary, a biopsy. hpvs were identified by a consensus-primer-based polymerase chain reaction (pcr) as ...	1998	9698486
human papillomavirus genotype as a major determinant of the course of cervical cancer.	to determine whether the prognosis of invasive cancers of the uterine cervix is related to the type of human papillomavirus (hpv) associated with the tumor.	1998	9704710
[unusual variant of laryngeal squamous cell carcinoma: verrucous carcinoma].	verrucous carcinoma is an uncommon but distinctive variety of squamous cell carcinoma, accounting for 1 to 2% of all laryngeal neoplasms. it was first described as a distinct clinicopathological entity by ackerman in 1948. it has been described most frequently in oral cavity and larynx, but other sites are possible. the preferred location of verrucous carcinoma of the larynx is supraglottic. its general similarity with papillomas and other papillomavirus-induced lesions has raised the question o ...	1998	9707746
metastatic cervical carcinoma mimicking kidney abscess.	a 26-year-old female, who had been treated for cervical carcinoma, presented with high fever and right flank pain. a right renal abscess was initially suspected from the clinical symptoms and diagnostic imaging. however, pathologic findings for the right kidney revealed squamous cell carcinoma, which was consistent in type with the original cervical carcinoma. demonstration of human papillomavirus 16 in tissues from both the renal tumor and the cervical carcinoma confirmed that the right kidney ...	1998	9712449
translational inhibition in vitro of human papillomavirus type 16 l2 mrna mediated through interaction with heterogenous ribonucleoprotein k and poly(rc)-binding proteins 1 and 2.	human papillomavirus (hpv) type 16 belongs to the group of "high risk" hpv types that are frequently detected in anogenital cancers. the expression of hpv-16 late genes encoding the virus capsid proteins l1 and l2 is restricted to terminally differentiated epithelial cells in the superficial layers of the squamous epithelium. we have previously identified negative elements in the 3' end of l2 rna that act in cis to reduce mrna utilization without substantially affecting mrna levels. the experime ...	1998	9712894
comparison of human papillomavirus type 18 (hpv-18) e6-mediated degradation of p53 in vitro and in vivo reveals significant differences based on p53 structure and cell type but little difference with respect to mutants of hpv-18 e6.	an important characteristic of the e6 proteins derived from oncogenic associated human papillomaviruses (hpvs) is their ability to target the cellular tumour suppressor protein, p53, for ubiquitin mediated degradation. several studies have attempted to address the important characteristics of both e6 and p53 for this activity in vitro, but the equivalent determinants have not been extensively assessed in vivo. indeed, recent studies indicate differences between the in vitro and the in vivo degra ...	1998	9714244
management of female genital warts with an analog of imiquimod 2% in cream: a randomized, double-blind, placebo-controlled study.	the purpose of this randomized, double-blind, placebo-controlled study was to determine the clinical efficacy and tolerability of an analog of imiquimod (2%)in cream to cure genital warts in women. sixty preselected women, ranging between 18 and 45 years of age (mean 24.3) and having 411 lesions (mean 6.8) with clinical, histopathological and polymerase chain reaction (pcr) confirmed diagnosis of human papilloma virus (hpv) infection were randomized to two parallel groups. each patient received ...	1998	9714974
uncoupling of p21waf1/cip1/sdi1 mrna and protein expression upon genotoxic stress.	the p21waf1/cip1/sdi1 gene is an important regulator of crucial cellular processes, including cell cycle control, cellular differentiation, and the response to genotoxic stress. induction of p21 gene expression upon dna damage is widely believed to be p53-dependent. in the present study we analysed the expression of p21 following genotoxic stress, using different dna-damaging agents and cellular systems. we found that the p21 response markedly varied between different cell lines and also for dif ...	1998	9715280
establishment of an epidermal growth factor-dependent, multipotent neural precursor cell line.	we have established a multipotent clonal cell line, named meb5, from embryonic mouse forebrains after the infection of a retrovirus carrying e7 oncogene of human papillomavirus type 16. meb5 cells proliferated in serum-free, epidermal growth factor (egf)-supplemented medium. they expressed markers for neural precursor cells (nestin, a2b5, and rc1) and did not express markers for neurons (class iii beta-tubulin), astrocytes (glial fibrillary acidic protein), and oligodendrocytes (galactocerebrosi ...	1998	9719419
suppression of senescence in normal human fibroblasts by introduction of dominant-negative p53 mutants or human papilloma virus type 16 e6 protein.	transfection of nearly senesced human fibroblasts with plasmids encoding hpv16 e6 protein or dominant-negative p53 mutants greatly increased their colony-forming ability. isolated colonies with these plasmids showed extension of lifespan compared to those with a control plasmid. these data demonstrate that p53 plays a major role in senescence in normal human fibroblasts.	1998	9720232
temporal and spatial expression of the e5a protein during the differentiation-dependent life cycle of human papillomavirus type 31b.	human papillomaviruses (hpvs) are epitheliotropic viruses, and their life cycle is intimately linked to the stratification and differentiation state of the host epithelial tissues. defining a role for the e5 gene product in the differentiation-dependent viral life cycle has been difficult due to the lack of a suitable culture system. we used the organotypic (raft) culture system to investigate the spatial and temporal expression pattern of the e5 protein during the differentiation-dependent life ...	1998	9721230
human papillomavirus type 31b e1 and e2 transcript expression correlates with vegetative viral genome amplification.	human papillomavirus (hpv) genome replication is dependent on the expression of e1 and e2 proteins. the organotypic (raft) culture system was used to investigate changes in viral early gene expression and vegetative genome replication during the complete life cycle of hpv type 31b (hpv31b). we have previously shown the synthesis of hpv31b viral particles as early as 10 days of growth of cin-612 9e raft tissues (ozbun, m. a., and meyers, c. (1997). j. virol. 71, 5161-5172). in the present study, ...	1998	9721231
the "inconclusive--possible high grade epithelial abnormality" category in papanicolaou smear reporting.	the australian terminology for cervical cytology reporting includes the category "inconclusive-possible high grade epithelial abnormality."	1998	9723595
early regression of cervical lesions in hiv-seropositive women receiving highly active antiretroviral therapy.	advanced hiv disease is associated with a high prevalence of cervical squamous intra-epithelial lesions (sil) and of infection with oncogenic human papillomavirus (hpv) genotypes. triple-combination antiretroviral therapy results in decreased plasma hiv viral load, increased cd4 cell counts and partial restoration of immune functions in patients with severe hiv disease. this study investigated the outcome of sil in hiv-seropositive women undergoing triple combination antiretroviral treatment.	1998	9727566
immunohistochemical co-expression of human papillomavirus type 16/18 transforming (e6) oncoprotein and p53 tumour suppressor gene proteins in oesophageal cancer.	human papillomaviruses have been widely implicated as important etiologic agents in various squamous cell carcinomas including oesophageal carcinoma. p53 mutant oncoprotein has also been implicated in various tumours. immunohistochemical analysis was employed to detect the co-expression of hpv and p53 mutant protein in biopsy specimens of patients of cancer oesophagus as well as controls. this analysis revealed a significantly higher immunopositivity (63%) of e6 oncoprotein of hpv 16/18 in carci ...	1998	9731468
risk of anal carcinoma in situ in relation to human papillomavirus type 16 variants.	infection with human papillomavirus (hpv), especially hpv16, is central to the development of squamous anogenital cancers and their precursor lesions, termed "squamous intraepithelial neoplasias." men who have sex with men, particularly those who are infected with hiv, are at a high risk for anal infection with hpv16 and for low-grade anal neoplasia; however, only a subset of these men develop anal invasive cancer or its immediate precursor lesion, anal carcinoma in situ (cis). to examine the hy ...	1998	9731493
nasal immunization of mice with human papillomavirus type 16 virus-like particles elicits neutralizing antibodies in mucosal secretions.	to specifically induce a mucosal antibody response to purified human papillomavirus type 16 (hpv16) virus-like particles (vlp), we immunized female balb/c mice orally, intranasally, and/or parenterally and evaluated cholera toxin (ct) as a mucosal adjuvant. anti-hpv16 vlp immunoglobulin g (igg) and iga titers in serum, saliva, and genital secretions were measured by enzyme-linked immunosorbent assay (elisa). systemic immunizations alone induced hpv16 vlp-specific igg in serum and, to a lesser ex ...	1998	9733865
activity of human papillomavirus type 16 p97 promoter in immortal and tumorigenic human oral keratinocytes.	we previously immortalized normal human oral keratinocytes (nhok) by transfection with cloned human papillomavirus type 16 (hpv-16) genome and converted these immortalized cells to tumorigenic cells with chemical carcinogens. since the tumorigenic cells expressed higher level of hpv-16 e6/e7 transcripts, we predicted that enhanced e6/e7 expression was induced by mutations at the long control region (lcr) of the viral genome integrated into cellular chromosome. to test this possibility, we sequen ...	1998	9735407
modulation of hpv16 e7 translation by trnas in eukaryotic cell-free translation systems.	translational regulation of hpv16 e7 mrna is of particular interest since the viral e7 protein has oncogenic activity. here we report that additional supplementation of cell-free reticulocyte/wheat-germ translation systems with rat liver trna pool favors the expression of the viral oncoprotein e7 which otherwise is scarcely translatable. the translational activation is explained by the positive correlation between the frequency of the glutamic and aspartic codons in e7 mrna, and the increased co ...	1998	9739465
the papillomavirus e6 proteins.	specific types of human papillomaviruses (hpv) are strongly associated with the development of cervical cancer. the e6 gene from cancer-related hpvs has exhibited functions in tumorigenesis, regulation of transcription, telomerase, and apoptosis. cancer-related hpvs e6 proteins bind the tumor suppressor p53 and promotes its degradation through an ubiquitin-dependent pathway. several additional cellular e6-binding proteins have recently been identified and implicated in playing roles in p53-indep ...	1998	9739758
morphological and immunohistochemical aspects of the biological seal in klöckner's dental implants: study of 15 cases.	the morphological and immunohistochemical aspects of the peri-implanted pathological soft tissue of 21 klöckner implants are assessed, corresponding to 15 patients and three gingival samples from three cadavers. for the collagen and vascular walls assessment, the picro-sirio technique is used with normal microscopy as well as with polarized light, evaluating the decrease of collagen fibers in relation to the grade of inflammation. in cases when there is a plasmocyte inflammatory prevalence, anti ...	1998	9743639
hpv-16 e7 protein bypasses keratinocyte growth inhibition by serum and calcium.	the e6 and e7 genes of hpv-16 or hpv-18 both are necessary for effective immortalization of primary human genital keratinocytes. to analyse the individual role of e6 and e7 genes in dysregulating cell growth, we cloned the hpv-16 e6, e7 and e6/e7 genes into retroviruses. primary human keratinocytes (phk) were then infected with these retroviruses and selected in differentiation-inducing medium (high calcium and serum). the e6/e7 retroviruses were the most effective at inducing differentiation-re ...	1998	9744546
establishment and characterization of a strial marginal cell line maintaining vectorial electrolyte transport.	e6/e7 genes of human papilloma virus type 16 were used to immortalize a primary culture of marginal cells (mc) from gerbils. one of the cloned lines was selected which demonstrated preservation of the main characteristics of the mc, both morphologically and physiologically. electron microscopic examination showed well-developed junctional complexes and apical microvilli which suggested its epithelial origin. polymerase chain reaction (pcr) demonstrated the incorporation of e6/e7 genes with the g ...	1998	9745959
evaluation of the detection of human papillomavirus genotypes in cervical specimens by hybrid capture as screening for precancerous lesions in hiv-positive women.	given the frequency and persistence of human papillomavirus (hpv) infection and associated cytological alterations in hiv-1-positive women, the incidence of uterine cervix neoplasm is likely to increase along with patient survival. more appropriate screening programs, which, in addition to pap smears (ps), also include tests to detect and type hpv, are needed for the early identification of precancerous cervical lesions. this prospective study involved 168 hiv-positive (group a) and 100 hiv-nega ...	1998	9746069
human papillomavirus and human cytomegalovirus dnas presence in patients suspected of condylomatosis or papillomatosis.	17 samples of total cell dna isolated from cervical smears from women suspected of condyloma or papilloma were analysed by pcr with appropriate primers, in order to establish the presence of viral dnas (hpv and/or hcmv). hpv dna was found in seven cases, and so was for hcmv dna. only in three cases a coinfection was present. the rflp allowed to specify the involvement of hpv6 in 3 cases suspected of condyloma and in one suspected of papilloma; the other three hpv positive samples had another gen ...	1998	10892429
[detection of human papillomavirus infection in cervical pap smears by computer-assisted cytologic test].	the sensitivity and accuracy of cytologic computer-assisted test (cct) in diagnosis of human papillomavirus (hpv) infection in cervical pap smear were evaluated.	1998	10682433
[the evaluation of computer cytological test with colposcopy for the diagnosis of cervical lesions].	to assess the value of computer cytological test (cct) with colposcopy for the diagnosis of early cervical diseases.	1998	10682434
[cloning transforming genes from human papillomavirus type 18].	nucleotide sequences of type 18 human papilloma virus genes e6 and e7 inserted in human dna cloned from cervical tumor are determined. the resultant sequences are compared to the prototype variant. five point mutations not leading to replacement of amino acid residues in polypeptide chain and 1 substituting the amino acid in codon 129 are detected in gene e6 sequence. in e7 sequence, one significant mutation in codon 92 is detected. both substitutions of asparagine for lysine are localized in th ...	1998	10488529
[study of human papillomavirus via chemiluminescence technic and polymerase chain reaction in transitional cell carcinoma of the bladder].	the exact ethiology of bladder carcinoma isn't yet known; an implication of human papillomavirus (hpv) has been recently hypothesized.	1998	10220998
detection of the e7 transform gene of human papilloma virus type 16 in human oral squamous cell carcinoma.	to determine, with the use of polymerase chain reaction, the prevalence of human papillomavirus (hpv) 16 in 30 patients with primary oral squamous cell carcinoma (oscc) and 30 healthy control patients.	1998	10557171
[the application of the polymerase chain reaction technic for the detection of human papillomavirus sequences].	the polymerase chain reaction technique was applied to detect sequences of human papillomavirus (hpv) by controls of cellular lines of cervical cancer and of tissues obtained through biopsy with a hpv-positive clinical diagnosis. a set of consensus oligonucleotides, which are complementary to a highly conserved region within the open reading frame e1 of the viral genome of hpv affecting the cervical mucosa, was used. with these primers it was possible to amplify dna sequences corresponding to hp ...	1998	10349442
latest opportunistic infection prevention guidelines.	although people with hiv are living longer than ever before, they continue to suffer from infections that are associated with low t4-cell counts. the u.s. public health service (phs) and the infectious diseases society of america (idsa) have updated the guidelines for preventing opportunistic infections, including their positions on when prophylaxis is indicated. summaries are given for preventing the following infections: cytomegalovirus (cmv), cryptosporidiosis, fungal infections, human papill ...	1998	11365000
human leukocyte derived interferon-alpha in a hydrophilic gel for the treatment of intravaginal warts in women: a placebo-controlled, double-blind study.	this placebo-controlled, double-blind study was aimed to evaluate the clinical efficacy, safety and tolerability of human leukocyte interferon-alpha (2 x 10(6) iu/g) incorporated in a hydrophilic gel (hydroxyethylcellulose, 1%) to cure intravaginal warts in women. preselected, subjects (n=60) who ranged between 18 and 50 years of age (mean 23.7), harbouring 275 vaginal warts (mean 4.6) with clinical, histopathological and polymerase chain reaction (pcr) confirmed diagnosis of human papillomaviru ...	1998	9874127