Publications

gas-liquid chromatographic analysis of fatty acid methyl esters of aeromonas hydrophila, aeromonas sobria, and aeromonas caviae.	clinical isolates of aeromonas hydrophila, a. sobria, and a. caviae whose fatty acid content had been analyzed by gas-liquid chromatography (glc) displayed the following qualitatively similar glc profiles: 12:0, 14:0, 15:0, 16:0, 17:0, 18:0, 16:1, 18:1, a-15:0, a-17:0, and 3-oh 14:0. the 16:0/17:0 area-percentage ratio separated the clinical aeromonads in accordance with their species designations. aeromonads treated with subminimal inhibitory concentrations of the antibiotic cerulenin displayed ...	1988	3366862
the growth of aeromonas hydrophila k144 in ground pork at 5 degrees c.	the influence of nacl, ph, atmosphere, and background microflora on the growth and/or survival of aeromonas hydrophila k144 was studied in ground pork held at 5 degrees c. in ground pork, a hydrophila was sensitive to ph values below 6.0 in the form of either a low starting ph in the pork itself or induced by lactic acid bacteria action on added glucose. growth of the organism is inhibited by nacl levels of 3% (w/w) (approx. 4% brine content). a hydrophila grew in vacuum-packaged ground pork; it ...	1988	3275309
surface protein composition of aeromonas hydrophila strains virulent for fish: identification of a surface array protein.	the surface protein composition of members of a serogroup of aeromonas hydrophila which exhibit high virulence for fish was examined. treatment of whole cells of representative strain a. hydrophila tf7 with 0.2 m glycine buffer (ph 4.0) resulted in the release of sheets of a tetragonal surface protein array. sodium dodecyl sulfate (sds)-polyacrylamide gel electrophoresis analysis showed that this sheet material was composed primarily of a protein of apparent molecular weight 52,000 (52k protein) ...	1988	3276660
isolation of aeromonas salmonicida from masu salmon in the republic of korea.	the isolation and identification of the gram-negative fish pathogen, aeromonas salmonicida salmonicida, from masu salmon (oncorhynchus masou) at the yang yang salmon hatchery in the republic of korea is described. the bacterium possessed certain characteristics in common with isolates from fish in japan. it is an important cause of mortality among cultured salmonids in the republic of korea. this is the first report of this fish pathogen from the asian mainland.	1988	3373645
preparation of metal-free enzymes.		1988	3374375
molecular cloning of a phospholipid-cholesterol acyltransferase from aeromonas hydrophila. sequence homologies with lecithin-cholesterol acyltransferase and other lipases.	we have determined the nucleotide sequence of a gene encoding aeromonas hydrophila phospholipid-cholesterol acyltransferase, an enzyme which shares many properties with mammalian lecithin:cholesterol acyltransferase. the derived amino acid sequence of the protein contains two regions which are homologous to the proposed active sites and binding sites of the plasma acyltransferase and to similar sequences in other interfacially acting lipolytic enzymes. the amino terminus is preceded by a typical ...	1988	3280033
an unusual case of pyogenic meningitis due to aeromonas sobria.		1988	3382842
aeromonas hydrophila gas gangrene: a case report of management with surgery and hyperbaric oxygenation.		1988	3282228
evaluation of several selective media for recovery of aeromonas hydrophila from polluted waters.	eleven media were studied for their suitability in the selective isolation of aeromonas hydrophila. preliminary results showed that five of them (inositol-brilliant green-bile salts agar, bile salts-brilliant green agar, rimler-shotts agar, xylose-sodium deoxycholate-citrate agar, and dextrin-fuchsin-sulfite agar) allowed the growth of several microorganisms that are usually present in the same samples in which a. hydrophila is found. six media (ma agar, modified rimler-shotts agar, dnase-toluid ...	1988	3214158
the effects of gamma irradiation on the lymphoid organs of rainbow trout and subsequent susceptibility to fish pathogens.	fish were irradiated with 60co gamma rays at doses ranging from 10 to 50 gy. lethal doses were determined in fishes of different ages. for a given dose, fry and fingerlings were more susceptible than subadults. whatever the irradiation dose was, the fish displayed a sharp decrease in blood leucocyte count. at the lowest doses, this acute leucopaenia was reversible. the cellular damage in the lymphoid organs was particularly obvious in the thymus. the depletion of lymphoid cells from immunocompet ...	1988	3388762
oxolinic acid in the trout: bioavailability and tissue residues.	a study was performed on the serum bioavailability and tissue elimination of oxolinic acid in the trout. the compound was added to the diet and administered at a dosage-level of 12 mg/kg/day for 7 consecutive days. the study utilized an analytical technique, high performance liquid chromatography, which has been described in detail here. the results obtained demonstrate that serum concentrations higher than the mic for the control of the target pathogens (aeromonas and yersinia) can by sustained ...	1988	3400972
[an epidemic of food poisoning by aeromonas hydrophila].		1988	3248456
pneumonia due to aeromonas hydrophila-complex: epidemiologic, clinical, and microbiologic features.	review of published data examining the various types of infections produced by strains of the aeromonas hydrophila-complex demonstrates a paucity of information on lower respiratory tract infections due to this organism. although it is rarely cited as a cause of pneumonitis, we have been able to collect epidemiologic, clinical, and microbiologic data on eight patients who have had evidence of aeromonas pneumonia. interestingly, seven of the eight patients were male, and the average age for the g ...	1988	3282317
partial purification and characterization of an extracellular proteinase from aeromonas hydrophila strain a4.	an extracellular metalloproteinase from aeromonas hydrophila strain a4, isolated from milk, was purified by a factor of 300 by chromatography on deae-cellulose and sephadex g-150. the enzyme had a mol. wt of 43,000 and contained 2 g atom ca/mol. it was active over a ph range 4.8-9.5 and had optimum activity on casein at ph 7.0 with km = 0.17 mm. it was strongly inactivated by metal chelators and the apoenzyme was fully reactivated with ca2+, mn2+ or co2+. heavy metal ions such as ag+, hg2+, fe2+ ...	1988	3290294
aeromonas-associated gastroenteritis in children.	during a 20-month period 55 strains of aeromonas species were isolated from 53 children with diarrhea. the isolation rate of 2.5% for aeromonas compared with the rates of 4.5% for shigella, 3.3% for salmonella, 2.7% for campylobacter and 0.05% for yersinia. in 45 children aeromonas was the sole bacterial enteropathogen identified. aeromonas was also isolated from 2 (0.5%) of 380 asymptomatic children. despite its known lack of identifiable virulence properties, aeromonas caviae was the most prev ...	1988	3340460
aeromonas hydrophila wound infection in a patient with cyclic neutropenia following a piranha bite.		1988	3340462
[prevalence of aeromonas spp. pediatric gastroenteritis].	a selective medium and biochemical tests were used to search for aeromonas spp. in the stools of 536 children, more than 90% of whom had "gastroenteritis", seen at sainte-justine hospital, montreal, in june or july 1986. the organism was found in seven children, six of whom had bloody diarrhea. one of the six, a 6 1/2-year-old boy, required intravenous alimentation and later manifested ulcerative colitis; the other five recovered. the remaining child, a 14-year-old boy, had synovitis of the knee ...	1988	3355951
structure of an s layer on a pathogenic strain of aeromonas hydrophila.	negative staining revealed a tetragonal surface array (s layer) on all the members of a serogroup of aeromonas hydrophila which possess high virulence for fish. the s layers were similar on all the strains examined, with unit cell dimensions of approximately 12 nm. a single representative strain, strain tf7, was selected for further analysis. freeze-cleaved and etched preparations and sections for electron microscopy showed that the s layer was the outermost component of the cell envelope. this ...	1988	3372478
effect of incubation temperature on growth and soluble protein profiles of motile aeromonas strains.	motile aeromonas spp. are said to have an optimal growth temperature of 28 degrees c. we performed growth studies on 24 isolates at 29 and 37 degrees c and found that the optimal growth temperature was not necessarily 28 degrees c. we also detected temperature-dependent differences in soluble protein production in some of these strains.	1988	3343335
isolation and biochemical characterization of the s-layer protein from a pathogenic aeromonas hydrophila strain.	the regular surface protein array (s layer) present on aeromonas hydrophila tf7 is composed of a single species of protein of apparent molecular weight 52,000. this protein was extracted from whole cells by treatment with 0.2 m glycine hydrochloride (ph 3.0). the protein was purified to homogeneity by ion-exchange chromatography and reverse-phase high-performance liquid chromatography. amino acid composition analysis showed that the protein contained 520 residues per molecule, 41% of which were ...	1988	3372479
[frequency of aeromonas species in the stools of healthy and diarrheic patients].	during two test periods (summer 1985 and january-august 1986) a total of 7713 stool specimens were examined for the occurrence of aeromonas spp. the isolation rate of the asymptomatic group was about 0.3%, the same rate was found in adults with diarrhea. in the stool specimens of 364 children 8 aeromonas spp. could be found. a. caviae was isolated in most cases of diarrhea. data obtained suggest that it seems to be useful to screen all diarrhoic stools of children for the occurrence of aeromonas ...	1988	3402999
surface properties of autoagglutinating mesophilic aeromonads.	the surface characteristics of 24 autoagglutinating (aa+) mesophilic aeromonads were investigated. one group of 16 was found to be highly related serologically by their reactive pattern against o antisera generated against three reference strains. subsequent characterization of 11 of these isolates (group 1) indicated that they had the following properties in common: precipitation after boiling (pab+), membership of serogroup o:11 (typing scheme of sakazaki and shimada), resistance to lysis by b ...	1988	3417353
[antibiotic resistance of several gram-negative bacteria from clinical and dissection material from the institute for poultry diseases of ludwig maximilian university, munich].	a survey is given on antibiograms carried out with important bacterial strains isolated from birds during 1987. as compared with the results from earlier years no significant changes could be shown, the sensitivity ranging from 42.4% to 95.9%. the genera bordetella and aeromonas were included for the first time.	1988	3420603
aeromonas eucrenophila species nova aeromonas caviae a later and illegitimate synonym of aeromonas punctata.	aeromonas caviae is a later and illegitimate synonym of aeromonas punctata. in order to secure continuity in literature, it is proposed to use the trivial term "caviae type" in connection with the species name a. punctata temporarily especially for strains isolated in connection to diarrhoea in children. aeromonas eucrenophila sp. nov. is described, type strain in ncmb 74. the new aerogenic psychrotrophic aeromonas species is separated genotypically from aeromonas hydrophila and aeromonas puncta ...	1988	3394446
screening of bacteria with antiviral activity from fresh water salmonid hatcheries.	bacteria isolated from two salmonid hatcheries were screened for antiviral activity against infectious hematopoietic necrosis virus (ihnv) to ascertain the presence of bacteria with anti-ihnv activity in the aquatic environment. out of 710 bacterial isolates from the water and sediment samples, 190 strains showed anti-ihnv activities of more than 50% plaque reduction. these antiviral activities were detected predominantly in pseudomonas, aeromonas/vibrio, and coryneforms. in one hatchery, the ba ...	1988	3374405
aeromonas hydrophila cellulitis. a case report.		1988	3385616
the phylogenetic origin of the bifunctional tyrosine-pathway protein in the enteric lineage of bacteria.	because bifunctional enzymes are distinctive and highly conserved products of relatively infrequent gene-fusion events, they are particularly useful markers to identify clusters of organisms at different hierarchical levels of a phylogenetic tree. within the subdivision of gram-negative bacteria known as superfamily b, there are two distinctive types of tyrosine-pathway dehydrogenases: (1) a broad-specificity dehydrogenase (recently termed cyclohexadienyl dehydrogenase [cdh]) that can utilize ei ...	1988	3386529
[analysis of degradative plasmids by pyrolysis gas liquid chromatography].		1988	3388828
[properties of aeromonas hydrophila enterotoxins from a food hygiene viewpoint].		1987	3566431
research on aeromonas and plesiomonas. iv. virulence factors.		1987	3552716
enteric pathogens in tropical aquaria.	a total of 100 tropical aquaria from six pet shops were sampled for the presence of salmonella, campylobacter and aeromonas species. eight fish tanks contained unusual serotypes of salmonella. none had been used to house terrapins. one hundred and three isolates of aeromonas species were obtained from 98 tanks. one tank contained plesiomonas shigelloides. no campylobacter species were isolated. it is suggested that a tropical aquarium may constitute an infection hazard.	1987	3428370
[spontaneous bacterial peritonitis caused by aeromonas hydrophila. presentation of 2 cases].		1987	3438545
intestinal infections with aeromonas.		1987	3544999
[pulmonary abscess: aeromonas hydrophila caused by a complication of drowning in fresh water].		1987	3450216
isolation frequency of aeromonas species in relation to patient age.	over a period of 18 months aeromonas spp. were isolated from stool specimens of 116 patients (total isolation frequency 1.6%). isolation rates in children up to ten years of age were significantly higher only for aeromonas caviae as compared to other age groups. additional data are needed to assess the clinical significance to these findings.	1987	3569253
research on aeromonas and plesiomonas. ii. non-gastrointestinal diseases.		1987	3569483
research on aeromonas and plesiomonas. iii. enteric infections.		1987	3569484
in vitro and in vivo study of an antimicrobial activity displayed by the redmouth disease agent, yersinia ruckeri.	a soluble antimicrobial product has been detected in yersinia ruckeri cultures. the contract droplets technique and the double agar layer method allowed to assess its bacteriostatic effect and the high susceptibility of other bacterial fish pathogens like vibrio and aeromonas. but it did not inhibit other strains of y ruckeri. no correlation was found between the antimicrobial activity and the virulence of the strains. as mixed infections with a salmonicida and y ruckeri could be obtained experi ...	1987	3579181
nucleotide sequence of the gene for the hole-forming toxin aerolysin of aeromonas hydrophila.	the gene for the hole-forming toxin aerolysin from aeromonas hydrophila was sequenced. although most of the sequence seems unrelated to that of staphylococcus aureus alpha-toxin, both proteins are very hydrophilic, and they each contain a nearly identical string of 10 amino acids.	1987	3584074
clinical and biochemical significance of toxin production by aeromonas hydrophila.	production of cytotoxin and enterotoxin by aeromonas strains obtained from stools of 50 children in mexico and texas and from blood of 9 children with sepsis was determined. results were correlated with clinical features of infected children as well as with biochemical traits of aeromonas strains. cytotoxin was produced by 40 of 42 aeromonas strains (95%) isolated from stools of children with diarrhea, by all 8 isolates from stools of well children, and by all 9 isolates from children with sepsi ...	1987	3584426
ampicillin-dextrin agar medium for the enumeration of aeromonas species in water by membrane filtration.	published selective media were evaluated for the isolation of aeromonas spp. from environmental samples by membrane filtration. satisfactory recoveries were obtained only with ma agar (rippey & cabelli) and dextrin-fuchsin-sulphite agar (schubert), but neither was sufficiently selective. the positive aspects of these two media were combined in a new medium, ampicillin-dextrin agar. recovery from pure cultures and environmental samples was optimal at an ampicillin concentration of 10 mg/l and inc ...	1987	3597206
cutaneous infections of the coast.		1987	3480441
class d tetracycline resistance determinants of r plasmids from the fish pathogens aeromonas hydrophila, edwardsiella tarda, and pasteurella piscicida.	tetracycline resistance determinants of r plasmids from the fish pathogens aeromonas hydrophila, edwardsiella tarda, and pasteurella piscicida were classified as class d by their expression of resistance to tetracycline and minocycline and through their dna structure.	1987	3631946
plasmids and resistance to antimicrobial agents in aeromonas sobria and aeromonas hydrophila clinical isolates.	the antimicrobial susceptibilities of 75 aeromonas isolates were determined by agar dilution. differences in resistance patterns were observed between strains isolated from different geographic locations and between a. sobria and a. hydrophila isolates. multiple resistance was common; however, only one conjugative plasmid was detected. this 110-megadalton plasmid mediated resistance to eight antibiotics.	1987	3631947
aeromonas hydrophila-associated colitis in a male homosexual.	a 37-year-old homosexual man was evaluated for a one-week history of hematochezia. results of a physical examination were remarkable only for grossly bloody stool. sigmoidoscopy to 30 cm showed a friable mucosa compatible with an acute colitis, and a rectal biopsy specimen demonstrated an increased plasma cell infiltrate. stool cultures subsequently yielded aeromonas hydrophila; serum human t-cell lymphotropic virus type iii antibody titer was positive. the patient responded to a course of treat ...	1987	3632156
growth of mixed microbial populations in ground water containing highly chlorinated organic wastes.		1987	3567405
l-methionine gamma-lyase from pseudomonas putida and aeromonas.		1987	3657560
detection of fish antibody against protein antigen of aeromonas salmonicida by enzyme-linked immunosorbent assay using biotin-avidin system.	antibody against aeromonas salmonicida was detected in sera from immunised or experimentally infected rainbow trout by enzyme-linked immunosorbent assay (elisa) using the biotin-avidin system. the elisa titre correlated well with the agglutinin titres of the sera, but the elisa was found to be more sensitive than the agglutination test. when the rainbow trout serum was separated by column chromatography, antibody activity (determined by elisa and agglutination test) was detected in the igm fract ...	1987	3628989
the bacteriological quality of bottled natural mineral waters.	fifty-eight bottles of natural mineral water, taken from the point of sale, were bacteriologically examined. no coliforms or aeromonas sp. were isolated from any sample. high total bacterial counts were found particularly in the still waters. most of the organisms isolated in the total counts were gram-negative rods, but gram-positive organisms were also isolated. gram-positive cocci were further identified, some of which were known human commensals suggesting contamination of the waters prior t ...	1987	3678404
virulence, cytotoxic and inflammatory activities of vibrio anguillarum and aeromonas salmonicida isolated from cultivated salmonid fish in sweden.		1987	3687646
comparison of selective media for primary isolation of aeromonas species from human and animal feces.	five selective media were evaluated for their effectiveness in the primary isolation of aeromonas spp. from human patients with acute diarrhea and from healthy domestic animals. sheep blood agar with 30 mg of ampicillin per liter (asba30) yielded a significantly higher percentage of positive specimens as compared with the four other media. the effective combination of two selective media with which 98% of all isolates were detected and with which all of the three human-associated aeromonas spp. ...	1987	3693536
identification of major common extracellular proteins secreted by aeromonas salmonicida strains isolated from diseased fish.	ten different strains of aeromonas salmonicida that were isolated from diseased fish were grown under identical conditions (24 h at 25 degree c) in 3% (wt/vol) tryptone soya broth medium supplemented with vitamins and inorganic ions. in each case the extracellular proteins that were formed were compared by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and it was found that there were two significant common components, one with a molecular weight of 70,000 and the other with a weight ...	1987	3555336
[septicemia caused by aeromonas hydrophila].		1987	3567701
[aeromonas hydrophila as an autochthonous causative agent of infectious enteritis in germany].	aeromonas hydrophila was identified by culture technique in diarrhoeal stools and ascites in a patient of 57 years of age who suffered from known alcoholic cirrhosis and additional acute gastroenteritis. no other pathogens that would produce acute diarrhoea were found. the isolated aeromonas hydrophila strain was characterised by the pathogenic properties known to date as pertaining to this species (formation of haemolysin and enterotoxins). hence it is evident that aeromonas hydrophila occurs i ...	1987	3608836
aeromonas hydrophila wound infection associated with myonecrosis and gas gangrene.		1987	3693881
acute diarrhoea in adults: a prospective study.	a prospective study of 74 adults with acute diarrhoea was carried out in sydney in 1984-1985 to determine the infective agents that were involved and their relationship to clinical, epidemiological and laboratory features. thirty-four potential pathogens were identified in 32 (43.2%) patients. these included, in order of frequency: campylobacter spp., rotavirus, clostridium difficile, salmonella enteritidis, aeromonas hydrophila, vibrio parahaemolyticus, adenovirus, a small round virus and giard ...	1987	3683260
congo red uptake by motile aeromonas species.	virulence of several species of enteropathogenic bacteria has been correlated with the ability of isolates to take up the dye congo red. to determine whether congo red uptake might be a useful marker for virulence of motile aeromonas species, we examined 50 strains of diverse clinical origin on a medium containing 50 micrograms of congo red per ml. all of the strains took up the dye to various degrees. for most strains, uptake was greatest at 37 degrees c and least at 22 degrees c. production of ...	1987	3584422
aeromonas veronii, a new ornithine decarboxylase-positive species that may cause diarrhea.	in 1983, the vernacular name enteric group 77 was coined for a group of strains that had been referred to our laboratory as "possible vibrio cholerae except for gas production." by dna-dna hybridization (hydroxyapatite, 32p), 8 of 10 strains of enteric group 77 were very highly related to the labeled strain 1169-83 (74 to 100% at 60 degrees c and 75 to 100% at 75 degrees c; percent divergence, 0.0 to 2.5). type strains of six other aeromonas species were 45 to 66% related (60 degrees c) to strai ...	1987	3584425
differentiation of motile and mesophilic aeromonas strains into species by testing for a camp-like factor.	motile and mesophilic aeromonas strains can presumptively be differentiated into species in 18 to 24 h by testing the isolates for the production of a camp-like factor. aeromonas hydrophila strains were positive either aerobically or anaerobically, aeromonas sobria strains were positive only aerobically, and aeromonas caviae strains were always negative.	1987	3611325
a modification of bile salts brilliant green agar for isolation of motile aeromonas from foods and environmental specimens.	aeromonas has recently been recognized as an important enteropathogen. in faecal specimens the organism is readily identified and differentiated from coliforms by the positive oxidase reaction. however, few media have proved useful for foods and environmental specimens. bile salts brilliant green starch agar (bbgs) was studied for this purpose by testing artificially contaminated foods as well as natural potential sources. the results indicate the suitability of the medium for the isolation of a ...	1987	3595749
aeromonas cytotonic enterotoxin cross reactive with cholera toxin.	isolation by affinity chromatography from crude culture filtrate of aeromonas sobria of protein that cross reacted with cholera toxin (ct) revealed a toxin that produced fluid accumulation in rat ileal loops and in infant mice and caused rounding of y1 adrenal cells. all these activities were neutralised by antiserum to ct. there was no haemolytic or cytotoxic activity associated with this ct-cross reactive cytotonic enterotoxin. ct-cross reactive material detected in enzyme linked immunosorbent ...	1987	3560188
enumeration and characterization of aeromonas hydrophila and aeromonas caviae isolated from grocery store produce.	starch-ampicillin agar was used to quantitatively isolate aeromonas sp. from retail grocery store produce. all produce sampled, including parsley, spinach, celery, alfalfa sprouts, broccoli, and lettuce, contained aeromonas sp. in most instances, the count of aeromonas sp. increased 10- to 1,000-fold during 2 weeks of storage at 5 degrees c. eleven (92%) of 12 kinds of produce yielded cytotoxic aeromonas sp. identification as aeromonas hydrophila was the strongest indicator of cytotoxicity, and ...	1987	3566266
activities of aeromonas hydrophila hemolysins and their interaction with erythrocyte membranes.	the activity of hemolysin produced by aeromonas hydrophila ca-11, isolated from an environmental source, was more sensitive to temperature than that of hemolysin produced by strain ah-1, isolated from a diarrheal case. ca-11 hemolysin failed to elicit hemolysis below 10 degrees c. immunoblotting analyses showed that both hemolysins formed into oligomers in rabbit erythrocyte membrane even when no hemolysis occurred. suggesting that the binding and the subsequent oligomerization are temperature i ...	1987	3596803
hydroxamate-induced spectral perturbations of cobalt aeromonas aminopeptidase.	the absorption spectrum of cobalt(ii)-substituted aeromonas aminopeptidase is markedly perturbed by the presence of equimolar concentrations of d-amino acid hydroxamates and acyl hydroxamates that have previously been shown to be powerful inhibitors of this enzyme (wilkes, s. h., and prescott, j. m. (1983) j. biol. chem. 258, 13517-13521). d-valine hydroxamate produces the most distinctive perturbation, splitting the characteristic 527 nm absorption peak of the cobalt enzyme to form peaks at 564 ...	1987	3597389
epidemiological complexity of hospital aeromonas infections revealed by electrophoretic typing of esterases.	an epidemiology analysis of a series of 12 aeromonas hydrophila infections, including six of septicaemia, which occurred on several wards of one hospital during the summer of 1982 is presented. the hypothesis that the hospital water could be the source of these infections was supported by the isolation of 1-10 motile aeromonads per ml in most of the water samples collected from various points on the hospital water system. electrophoretic esterase typing was used as an epidemiological screening m ...	1987	3556437
pyomyositis and hepatic abscess in association with aeromonas hydrophila sepsis.	this report describes a patient with chronic liver disease in whom aeromonas hydrophila sepsis developed following ingestion of fresh lake water. a hepatic abscess and bilateral calf pyomyositis, an extremely rare and previously fatal complication of a. hydrophila septicemia, subsequently developed. the patient recovered following bilateral fasciotomies and systemic antibiotic therapy with gentamicin and trimethoprim/sulfamethoxazole. no previous survivors of a. hydrophila pyomyositis secondary ...	1987	3618636
the effect of different brands of macconkey and xylose lysine deoxycholate agar on the isolation of aeromonas hydrophila.	aeromonas hydrophila has been reported to be involved in the opportunistic infection of human beings, therefore, its rapid diagnosis from the clinical specimens was considered to be extremely important for the prognosis. the effect of different brands of macconkey (mac) and xylose lysine deoxycholate (xld) agars on the plating efficiency and colony size of a. hydrophila was evaluated by comparing with blood agar. results indicate that (i) except eiken's mac have low plating efficiency, all the m ...	1987	3428016
binding of clavulanic acid to the penicillin-binding proteins of aeromonas hydrophila.		1987	3509501
[study of the sensitivity of enteropathogenic bacteria to nifurzide].	nifurzide, a synthetic antimicrobial agent of the nitrofuran group, was tested on 320 enteropathogenic bacteria. minimal inhibition concentrations (mic) (expressed in mg/l) were determined using the standard agar dilution technique. the mics were excellent for campylobacter spp. (97 strains), mic 50:0.5, mic 90:0.5; slightly higher for shigella spp. (50 strains) and aeromonas spp. (22 strains), mic 50:2, mic 90:4; and highest for salmonella spp. (100 strains) and yersinia enterocolitica (49 stra ...	1987	3302859
in vitro activity of ci-934 compared with ciprofloxacin, enoxacin, norfloxacin, and vancomycin.	the in vitro activity of the quinolone ci-934 was compared with ciprofloxacin, norfloxacin, enoxacin, and vancomycin against 607 gram-positive and -negative isolates. ci-934 inhibited 90% of the enterobacteriaceae, aeromonas hydrophila, and acinetobacter spp. at 1 microgram/ml. decreased activity was observed against pseudomonas aeruginosa. pseudomonas maltophilia, and other pseudomonas spp. with ci-934 mic90 greater than or equal to 8 micrograms/ml. ci-934 activity against gram-positive organis ...	1987	3472704
marker exchange mutagenesis of the aerolysin determinant in aeromonas hydrophila demonstrates the role of aerolysin in a. hydrophila-associated systemic infections.	we report here on the isolation of isogenic strains of aeromonas hydrophila ab3 deleted for a segment of the aerolysin gene. all aer mutants obtained lacked the 49-kilodalton aerolysin gene product and were neither hemolytic for blood erythrocytes nor cytotoxic for chinese hamster ovary tissue culture cells. one such mutant, ab3-5, was used in a mouse toxicity model to evaluate the role of aerolysin in the pathogenesis of a. hydrophila infections. the strain had a 50% lethal dose (ld50) of great ...	1987	3305370
[aeromonas hydrophila as the causative agent of ileocolitis].		1987	3315547
[aeromonas spp. as pathogens in human infections: diagnosis and clinical significance].	aeromonads have been described as causative agents in a wide variety of infections in immunosuppressed as well as in immunocompetent patients. it is still controversial whether or not some or all three currently recognized species have to be considered as enteropathogenic. clinical and epidemiological data are incomplete and contradictory although laboratory diagnosis does not cause real problems.	1987	3315971
[fatal aeromonas septicemia in two patients with acute myelogenous leukemia: clinical significance in neutropenic states and review of the literature].		1987	3323564
evaluation of a small "conventional" identification system for fermentative gramnegative rods using a computerized data base.	a small "conventional" identification system consisting of six tubed media totalling eleven reactions was used to identify 467 strains of enterobacteriaceae, 15 strains of aeromonas sp., and 3 strains of plesiomonas shigelloides. the system identified 371 strains (76.5%) correctly to maximum identification (species or genus) level and an additional 13 strains (2.7%) to genus level where species level identification would have been possible. ninety strains (18.6%) were not identified. only 11 str ...	1987	3326369
phenotypic differentiation of members of the family vibrionaceae using miniaturized biochemical tests.	enzymatic hydrolysis of 27 different chromogenic substrates and the assimilation of 44 carbon sources by 144 strains of vibrio species of clinical importance, aeromonas hydrophila and plesiomonas shigelloides were studied by standardized micromethods. some classical biochemical tests were also performed using the test kit tte-as (flow laboratories gmbh, meckenheim, frg). reading of results was done automatically by a photometer and test data were recorded and stored by a microcomputer. all speci ...	1987	3326370
lymphadenopathy syndrome and hiv infection in multitransfused beta-thalassemia child.	lymphoadenopathic syndrome (las), diagnosed also with histologycal studies, is described in a polytransfused 11-year old boy affected by beta-thalassemia major. after three days of the admission he suffered a serious acute diarrhea by aeromonas hydrophila, an opportunistic enteric bacteria demonstrated, up to date, in immunocompromised patients and never in aids patients. it is important to remark that beta-thalassemic patients are at high risk from hiv infections, either for the chronic need of ...	1987	3435653
[a case of aeromonas hydrophila sepsis with generalized gas-formation and intravascular hemolysis].		1987	3334425
two-hour miniaturized system for detection of enteropathogenic bacteria in stool cultures.	a miniaturized 2-h system for detection of lactose-negative potentially enteropathogenic bacteria on the basis of nine enzymatic tests was evaluated. the system was challenged with 210 strains of lactose-negative and lactose-positive species grown on macconkey agar. specific and constant patterns were found for salmonella, shigella a-c, yersinia enterocolitica, aeromonas spp. and vibrio cholerae. shigella sonnei and hafnia alvei had an identical pattern, likewise plesiomonas shigelloides, haloph ...	1987	3440458
[geographic variations of the isolation of aeromonas hydrophila in infants with acute infantile diarrhea and a healthy population].		1987	3444950
bacterial flora in bottled uncarbonated mineral drinking water.	a quantitative study of bacterial populations in mineral water was carried out. samples were stored at 6 and 20 degrees c, and the colony counts were determined on tryptone agar plates incubated at 22 and 37 degrees c. samples were collected from the spring source in sterile glass flasks and from the bottling factory in conventional plastic and glass containers. in both cases, the initial population (10(1)-10(2) cfu/ml water) increased to 10(5)-10(6) cfu/ml after 3 days storage as determined fro ...	1987	3446349
natural microbiologic hazards of the aquatic environment.		1987	3311341
[tte-ras (titertek-enterobac-ras), a new micromethod for the semi-automatic identification of enterobacteriaceae within 5 hours].	titertek-enterobac-ras (tte-ras), a new semi-automated system for the identification of the enterobacteriaceae-within five hours, has been evaluated and compared with conventional methods. this note presents the results upon 655 strains, mainly enterobacteriaceae tte-ras provided correct identification for about 92 p. cent and 97 p. cent of enterobacteriaceae respectively before and after carrying out supplementary tests according to manufacturer's instruction. tte-ras gave 97 p. cent specific r ...	1987	3314607
does aeromonas hydrophila preferentially colonize the bowels of patients with hematologic malignancies?	weekly cultures of stools from neutropenic patients and bone marrow transplant recipients yielded aeromonas hydrophila from 8% of 88 patients over a 2-yr period. during this time stools in the routine enteric laboratory yielded a. hydrophila in 0.24% of 1632 patients. although the patient groups and culture methods were not directly comparable, this significant difference in isolation rate (p less than 0.001) may reflect a higher colonization rate in the immunocompromised patient.	1987	3319373
marine bacteria which produce tetrodotoxin.	a number of type strains of marine bacteria, including members of the family vibrionaceae, were cultured and examined for tetrodotoxin productivity by high-performance liquid chromatography and gas chromatography-mass spectrometry. most of the vibrionaceae strains produced tetrodotoxin, anhydrotetrodotoxin, or both.	1987	3310884
medical leeches as sources of wound infection.		1987	3107664
isolation of aeromonas sp from faecal specimens.		1987	3693573
aeromonas in the gut.		1987	3693922
enlarging erythematous macule: a cutaneous sign for fatal aeromonas hydrophila septicemia.		1987	3625077
bacteriology of the freshwater environment: implications for clinical therapy.	water and animal tissue samples were obtained from sources in tennessee, california, and florida. purified bacterial colonies were isolated and organisms identified. fifty-eight isolates were recovered. twenty-seven gram-negative isolates were identified. gram-positive organisms were of the coryneform group or bacillus species. antibiotic susceptibility testing showed that aeromonas species were relatively resistant to a wide variety of antimicrobials, which included trimethoprim, cefazolin, and ...	1987	3631666
[vibrio and aeromonad sensitivity to the vibriostatic o 129].	sensitivity of vibrios and aeromonads to vibriostatic o129 (2,4-diamino-6,7-diisopropyl pteridine) was studied. the vibrios and aeromonads were isolated in the ussr. the tests were performed with disks made in the ussr and great britain. the content of the drug in the disks was 10 and 150 micrograms. it was shown that v. cholerae o1 v. cholerae not o1 and v. albensis were highly sensitive to vibriostatic o129 and produced no growth in the presence of either low or high concentrations of the drug ...	1987	3631937
typing of aeromonas species by polyacrylamide-gel electrophoresis of radiolabelled cell proteins.	a method for typing species of aeromonas by 35s-labelling and sodium-dodecyl sulphate polyacrylamide-gel electrophoresis (sds-page) of total soluble proteins is described. radiolabelled-protein profiles from different strains were compared by use of the dice coefficient. typability was 100% and discrimination of pairs of differing strains was satisfactory with acceptable reproducibility. the results also yielded information on subgroups of phenotypic species.	1987	3656393
seafood shucking as an etiology for aeromonas hydrophila infection.	aeromonas hydrophila has been increasingly documented as a hardy organism responsible for severe infection in the compromised host. this case report illustrates how a hydrophila may survive prolonged freezing and how seafood shucking may cause sepsis. this report serves as a caution to the immunocompromised seafood lover.	1987	3662710
detection, quantitation and stability of the beta haemolysin of aeromonas spp.	amongst 58 isolates of motile aeromonads evaluated for the ability to produce beta haemolysin, haemolytic activity was significantly associated with strains belonging to the aeromonas hydrophila and a. sobria groups. of erythrocytes from nine animal species tested, mouse red blood cells provided the best indicator system for detection of beta-haemolysin activity. furthermore, differences in the stability of the beta haemolysins of selected a. sobria and a. hydrophila isolates at different temper ...	1987	3669057
virulence markers of mesophilic aeromonads: association of the autoagglutination phenomenon with mouse pathogenicity and the presence of a peripheral cell-associated layer.	autoagglutination (aa phenotype) of mesophilic aeromonads in broth was found to be a virulence-associated marker. there were two kinds of aa+ strains: those that spontaneously pelleted (sp+), and those that pelleted only after boiling (pab+). of 79 strains tested, 24 (30%) were aa+, and 18 of these were recovered from clinical specimens. most of the aa+ strains (n = 21) were identified as either aeromonas sobria or aeromonas hydrophila. of the well-documented clinical isolates of a. sobria and a ...	1987	3679544
clinical significance of aeromonas species isolated from patients with diarrhea.	a total of 248 strains of aeromonas spp. were isolated from 3,334 human fecal specimens submitted to a state public health laboratory over a 2-year period to be cultured for enteric pathogens. cary-blair transport medium, blood ampicillin agar, and alkaline peptone water enrichment provided optimal recovery of aeromonas spp. a questionnaire requesting clinical and epidemiological information was sent to physicians, who submitted stool samples for testing, with each laboratory report for 107 cons ...	1987	3693537
evaluation of quantum ii microbiology system for identification of gram-negative bacteria of veterinary origin.	the ability of a rapid, semiautomated bacterial identification system, the quantum ii microbiology system (abbott laboratories, irving, tex.), to accurately identify gram-negative bacteria from veterinary sources was evaluated. a total of 378 isolates were tested, including 298 organisms in the family enterobacteriaceae and strains representing acinetobacter sp., aeromonas sp., flavobacterium sp., pasteurella multocida, plesiomonas sp., and pseudomonas spp. of these isolates, 333 (88.1%) were co ...	1987	3693539
interaction of thromboxane a2 and tissue pathology during graded bacteremia.	the purpose of this study was to determine whether or not thromboxane a2 (txa2) was necessary or sufficient for the development of end-organ pathology during graded bacteremia. pulmonary artery catheters were placed in 21 adult male pigs under pentobarbital anesthesia and breathing room air. after a control period, animals were studied in four groups: group 1, anesthesia only; group 2, infusion of 1 x 10(9) ml aeromonas hydrophila which was gradually increased from 0.2 ml/kg/hr to 4.0 ml/kg/hr o ...	1987	3820352
aeromonas-associated diarrhea in the netherlands.		1987	3826975
importance of aeromonas sobria in aeromonas bacteremia.		1987	3805781