Publications

pfeiffer's bacillus and influenza : a serological study.	it has been shown that the sera of patients convalescent from influenza yield reactions for agglutinins, precipitins, and complement-binding bodies with antigens of pfeiffer's bacillus. these reactions appear constantly at the end of the 1st week, increase in intensity during the 2nd week, and remain demonstrable for a period of 2 to 4 months. they were most complete in the sera of patients suffering from postinfluenzal pneumonia. it has also been demonstrated that the strains of pfeiffer's baci ...	1919	19868377
preventive treatment in influenza: a british medical association lecture given to the southern branch, at southampton, november 13th, 1919.		1919	20769715
the etiology of influenza: a filtrable virus as the cause, with some notes on the culture of the virus by noguchi's method.		1919	20769413
a filtrable virus as the cause of the early stage of the present epidemic of influenza: (a preliminary note.).		1918	20769290
acute inflammation of the left antrum of highmore after influenza: a personal observation.		1894	20754648
experimental vaccines against potentially pandemic and highly pathogenic avian influenza viruses.	influenza a viruses continue to emerge and re-emerge, causing outbreaks, epidemics and occasionally pandemics. while the influenza vaccines licensed for public use are generally effective against seasonal influenza, issues arise with production, immunogenicity, and efficacy in the case of vaccines against pandemic and emerging influenza viruses, and highly pathogenic avian influenza virus in particular. thus, there is need of improved influenza vaccines and vaccination strategies. this review di ...	0	23440999
antiviral activity of binase against the pandemic influenza a (h1n1) virus.	the lack of effective antiviral drugs restricts the control of the dangerous rna-containing influenza a (h1n1) virus. extracellular ribonuclease of bacilli (binase) was shown to manifest antiviral activity during single- and multi-cycle viral replication in the range of concentrations non-toxic to epithelial cells and 0.01-0.1 multiplicity of infection. during antiviral treatment for 15-30 min, the concentration of 1 μg/ml binase reduced the amount of focus-forming units of viruses by a factor o ...	0	24455182
improved immunogenicity of individual influenza vaccine components delivered with a novel dissolving microneedle patch stable at room temperature.	prevention of seasonal influenza epidemics and pandemics relies on widespread vaccination coverage to induce protective immunity. in addition to a good antigenic match with the circulating viruses, the effectiveness of individual strains represented in the trivalent vaccines depends on their immunogenicity. in this study, we evaluated the immunogenicity of h1n1, h3n2, and b seasonal influenza virus vaccine strains delivered individually with a novel dissolving microneedle patch and the stability ...	0	25895053
emerging infections of cns: avian influenza a virus, rift valley fever virus and human parechovirus.	history is replete with emergent pandemic infections that have decimated the human population. given the shear mass of humans that now crowd the earth, there is every reason to suspect history will repeat itself. we describe three rna viruses that have recently emerged in the human population to mediate severe neurological disease. these new diseases are results of new mutations in the infectious agents or new exposure pathways to the agents or both. to appreciate their pathogenesis, we summariz ...	0	26276027
serologic study of pig-associated viral zoonoses in laos.	we conducted a serologic survey of four high-priority pig-associated viral zoonoses, japanese encephalitis virus (jev), hepatitis e virus (hev), nipah virus (niv), and swine influenza virus (siv), in laos. we collected blood from pigs at slaughter during may 2008-january 2009 in four northern provinces. japanese encephalitis virus hemagglutination inhibition seroprevalence was 74.7% (95% confidence interval [ci] = 71.5-77.9%), jev igm seroprevalence was 2.3% (95% ci = 1.2-3.2%), and hev seroprev ...	0	22665622
(in)validating experimentally derived knowledge about influenza a defective interfering particles.	a defective interfering particle (dip) in the context of influenza a virus is a virion with a significantly shortened rna segment substituting one of eight full-length parent rna segments, such that it is preferentially amplified. hence, a cell co-infected with dips will produce mainly dips, suppressing infectious virus yields and affecting infection kinetics. unfortunately, the quantification of dips contained in a sample is difficult because they are indistinguishable from standard virus (stv) ...	0	27881801
evolution-guided identification of antiviral specificity determinants in the broadly acting interferon-induced innate immunity factor mxa.	mxa is an interferon-induced dynamin-like gtpase with wide-ranging antiviral activity, which hinges upon detection of unique viral structures that differ across virus families. despite elucidation of its structure, the basis of mxa antiviral specificity remains enigmatic. we used an evolution-guided approach to identify the loop l4 of mxa as a hotspot for recurrent positive selection in primates. further, we show that single amino acid changes in l4 are necessary and sufficient to explain dramat ...	0	23084925
ifitm-family proteins: the cell's first line of antiviral defense.	animal cells use a wide variety of mechanisms to slow or prevent replication of viruses. these mechanisms are usually mediated by antiviral proteins whose expression and activities can be constitutive but are frequently amplified by interferon induction. among these interferon-stimulated proteins, members of the ifitm (interferon-induced transmembrane) family are unique because they prevent infection before a virus can traverse the lipid bilayer of the cell. at least three human ifitm proteins-i ...	0	25599080
development and characterization of neutralizing monoclonal antibodies against the s1 subunit protein of qx-like avian infectious bronchitis virus strain sczy3.	infectious bronchitis (ib) is a highly contagious disease in chickens caused by infectious bronchitis virus (ibv). the present study was carried out with the aim to develop anti-spike 1 (s1) subunit monoclonal antibodies (mabs) that could react with ibv strains of different genotypes. the high antigenicity region of s1 gene of an qx-like ibv strain sczy3 was amplified and ligated into the prokaryotic expression vector pet-32a(+), and the recombinant his-s1 fusion proteins were expressed and puri ...	0	25723279
reduced interferon-α production by dendritic cells in type 1 diabetes does not impair immunity to influenza virus.	the increased risk and persistence of infections in diabetic condition is probably associated with defects in the cellular immune responses. we have previously shown a decrease in the production of interferon (ifn)-α by dendritic cells (dcs) in diabetic subjects. the basal level of ifn-α in splenic plasmacytoid dcs (pdcs) is also lower in non-obese diabetic (nod) mice compared to prediabetic mice. the objective of this study was to analyse the ability of diabetic mice to mobilize innate and cd8( ...	0	25286929
passive immune neutralization strategies for prevention and control of influenza a infections.	although vaccination significantly reduces influenza severity, seasonal human influenza epidemics still cause more than 250,000 deaths annually. vaccine efficacy is limited in high-risk populations such as infants, the elderly and immunosuppressed individuals. in the event of an influenza pandemic (such as the 2009 h1n1 pandemic), a significant delay in vaccine availability represents a significant public health concern, particularly in high-risk groups. the increasing emergence of strains resis ...	0	22339460
immunological considerations for developing antibody therapeutics for influenza a.	influenza infection can give rise to serious illness leading to complications and hospitalization of patients. the efficacy of current standard of care is very limited and provides little relief for patients hospitalized with serious flu. human monoclonal antibodies (mab) against influenza are being developed as new treatment options for this patient population. when developing antibody therapeutics, it is important to consider all possible immunologic effects of the antibodies on viral infectio ...	0	26325257
virus evolution and transmission in an ever more connected world.	the frequency and global impact of infectious disease outbreaks, particularly those caused by emerging viruses, demonstrate the need for a better understanding of how spatial ecology and pathogen evolution jointly shape epidemic dynamics. advances in computational techniques and the increasing availability of genetic and geospatial data are helping to address this problem, particularly when both information sources are combined. here, we review research at the intersection of evolutionary biolog ...	0	26702033
use of observed wild bird activity on poultry farms and a literature review to target species as high priority for avian influenza testing in 2 regions of canada.	the risk of avian influenza outbreaks in poultry is partially dependent on the probability of contact between domestic poultry and wild birds shedding avian influenza (ai) virus. the major objective of this study was to document wild bird activity on poultry farms to determine which wild bird species should be targeted for ai surveillance in canada. we collected data in 2 major poultry producing regions of canada, southwestern ontario and the fraser valley of british columbia, on the relative ab ...	0	22851777
anxiety and depression: linkages with viral diseases.	anxiety and mood disorders are common in the general population in countries around the world. this article provides a review of the recent literature on anxiety and depressive disorders with a focus on linkages with several important viral diseases. although the majority of studies have been conducted in developed countries such as the united states and great britain, some studies have been carried out in less developed nations where only a small percentage of persons with mental illness receiv ...	0	25264396
interferon-induced transmembrane protein-3 genetic variant rs12252-c is associated with severe influenza in chinese individuals.	the snp rs12252-c allele alters the function of interferon-induced transmembrane protein-3 increasing the disease severity of influenza virus infection in caucasians, but the allele is rare. however, rs12252-c is much more common in han chinese. here we report that the cc genotype is found in 69% of chinese patients with severe pandemic influenza a h1n1/09 virus infection compared with 25% in those with mild infection. specifically, the cc genotype was estimated to confer a sixfold greater risk ...	0	23361009
protective efficacy of orally administered, heat-killed lactobacillus pentosus b240 against influenza a virus.	influenza a(h1n1)pdm virus caused the first human pandemic of the 21st century. although various probiotic lactobacillus species have been shown to have anti-microbial effects against pneumonia-inducing pathogens, the prophylactic efficacy and mechanisms behind their protection remain largely unknown. here, we evaluated the prophylactic efficacy of heat-killed lactobacillus pentosus b240 against lethal influenza a(h1n1)pdm virus infection in a mouse model. to further define the protective respon ...	0	23535544
stable isotopes suggest low site fidelity in bar-headed geese (anser indicus) in mongolia: implications for disease transmission.	population connectivity is an important consideration in studies of disease transmission and biological conservation, especially with regard to migratory species. determining how and when different subpopulations intermingle during different phases of the annual cycle can help identify important geographical regions or features as targets for conservation efforts and can help inform our understanding of continental-scale disease transmission. in this study, stable isotopes of hydrogen and carbon ...	0	27695389
downregulating viral gene expression: codon usage bias manipulation for the generation of novel influenza a virus vaccines.	vaccination represents the best option to protect humans against influenza virus. however, improving the effectiveness of current vaccines could better stifle the health burden caused by viral infection. protein synthesis from individual genes can be downregulated by synthetically deoptimizing a gene's codon usage. with more rapid and affordable nucleotide synthesis, generating viruses that contain genes with deoptimized codons is now feasible. attenuated, vaccine-candidate viruses can thus be e ...	0	26213563
species difference in anp32a underlies influenza a virus polymerase host restriction.	influenza pandemics occur unpredictably when zoonotic influenza viruses with novel antigenicity acquire the ability to transmit amongst humans. host range breaches are limited by incompatibilities between avian virus components and the human host. barriers include receptor preference, virion stability and poor activity of the avian virus rna-dependent rna polymerase in human cells. mutants of the heterotrimeric viral polymerase components, particularly pb2 protein, are selected during mammalian ...	0	26738596
one health, multiple challenges: the inter-species transmission of influenza a virus.	influenza a viruses are amongst the most challenging viruses that threaten both human and animal health. influenza a viruses are unique in many ways. firstly, they are unique in the diversity of host species that they infect. this includes waterfowl (the original reservoir), terrestrial and aquatic poultry, swine, humans, horses, dog, cats, whales, seals and several other mammalian species. secondly, they are unique in their capacity to evolve and adapt, following crossing the species barrier, i ...	0	26309905
new polymorphism of the influenza virus resistance mx1 gene in iberian domestic pigs.	mx1 (myxovirus (influenza virus) resistance 1, interferon-inducible protein p78) gene has been implicated in the resistance to a wide range of rna viruses including influenza a in several species such as sus scrofa. in the present study a 28-bp deletion in exon 14 of the mx1 gene has been identified in iberian domestic pigs but not in other domestic breeds neither in wild boars. the mutation produces a frameshift giving a protein with 6 amino acid substitutions and the extension of the c-termina ...	0	27774491