Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| undetectable ige responses after respiratory syncytial virus infection. | sequential nasopharyngeal secretions were collected from 81 infants from one day to three months after admission to hospital with respiratory syncytial virus (rsv) infection. samples from 21 infants were assayed for anti-rsv ige in an antigen capture elisa assay. no ige antibodies were detected although an assay of iga antibodies carried out in parallel by a similar technique detected iga antibodies in the secretions of all patients tested. neither prior absorption of iga or igg, concentration o ... | 1996 | 8660074 |
| respiratory syncytial virus infection in children with congenital heart disease: a review. | this paper reviews recent changes in morbidity and mortality of respiratory syncytial virus (rsv) infection in infants with congenital heart disease. mortality since the late 1970s has declined substantially, from approximately 37% to 3%. although the frequency of admission to intensive care units has declined from approximately 60% to 30%, the frequency for mechanical ventilatory support has not changed significantly. because mortality dropped prior to the widespread use of ribavirin, it is dif ... | 1996 | 8662029 |
| a20 blocks endothelial cell activation through a nf-kappab-dependent mechanism. | the a20 gene product is a novel zinc finger protein originally described as a tumor necrosis factor alpha (tnf)-inducible early response gene in human umbilical vein endothelial cells (huvec). its described function is to block tnf-induced apoptosis in fibroblasts and b lymphocytes, but more recently it has also been shown to play a role in lymphoid cell maturation. the mechanism of action of a20 is unknown. the aim of our study was to assess the effect of a20 upon endothelial cell activation. b ... | 1996 | 8663499 |
| evaluation of four methods for the diagnosis of respiratory syncytial virus infection in older adults. | to evaluate four methods of rapid diagnosis of respiratory syncytial virus (rsv) infection in older adults and to compare sensitivities with serologic analysis. | 1996 | 8537595 |
| vitamin a and respiratory syncytial virus infection. serum levels and supplementation trial. | to determine the benefit of oral vitamin a supplementation for acute respiratory syncytial virus (rsv) infection. | 1996 | 8542002 |
| rsv infection complicating the therapy of pediatric malignancies: report of six cases. | we describe a series of six patients with symptomatic respiratory syncytial virus (rsv) infections while receiving anticancer chemotherapy. particularly during epidemics in the general population, rsv remains a potential cause for morbidity and even mortality among children immunocompromised through the administration of anticancer chemotherapy and especially those being transplanted. we emphasize the importance of rapid diagnostics as well as prevention of the spread of the virus in a pediatric ... | 1996 | 8600339 |
| respiratory infection and iatrogenic diarrhea in honduras and el salvador during the 1991-1992 season. | the etiology of acute respiratory tract infection and its association with diarrhea was analyzed in 135 hospitalized children less than three years of age with mainly respiratory symptoms in two pediatric hospitals in honduras and el salvador. etiologic diagnoses were performed on nasopharyngeal samples by tissue culture and immunofluorescence, including a search for the presence of respiratory virus-specific immunoglobulin a antibodies. fecal samples were subjected to electron microscopy and ti ... | 1996 | 8600762 |
| non-viral sequences at the 5' termini of mrnas derived from virus-sense and virus-complementary sequences of the ambisense rna segments of rice stripe tenuivirus. | the three small segments of the four rnas of the rice stripe tenuivirus (rsv) genome have an ambisense coding strategy. the mrna transcripts corresponding to open reading frames for the non-structural protein (ns4) and nucleocapsid protein (n), which are encoded on virus-sense (v) rna 4 and virus-complementary sense (vc) rna 3, respectively, were recovered from polysomes of rsv-infected wheat leaves, and their 5' termini were analysed. the mrnas derived from both v and vc sequences contained fro ... | 1996 | 8601793 |
| expression of exogenous protein and analysis of morphogenesis in the developing chicken heart using an adenoviral vector. | recombinant retroviral vectors have been shown to be useful tools for marking cells so as to follow their fates during development. the aim of this study was to determine the utility and advantages of an adenoviral vector as a tool to study the heart as it develops from a simple tube into a complex four-chambered organ. | 1996 | 8681350 |
| [an epidemic of respiratory syncytial virus bronchiolitis among infants in northern israel]. | in the wake of a community outbreak of bronchiolitis in northern israel from december 1993 to march 1994, we conducted a retrospective study of 108 infants aged 2 weeks to 14 months with proven respiratory syncytial virus (rsv) infection (diagnosed by a rapid rsv antigen test in nasopharyngeal secretions). 47% of the infants were less than 8 weeks old. mean hospital stay was 6.6 days (range 1-60). the characteristic clinical findings were: cough in all patients, dyspnea in 96%, rhinitis in 95% a ... | 1996 | 8682391 |
| proposed three-dimensional model for the attachment protein g of respiratory syncytial virus. | protein g of respiratory syncytial virus (rsv) is an envelope glycoprotein that is structurally very different from its counterparts (haemagglutinin-neuraminidase and haemagglutinin) in other paramyxoviruses. in this study, we put forward a model for this unique viral envelope protein. we propose that protein g of rsv contains several independently folding regions, with the ectodomain consisting of a conserved central hydrophobic region located between two polymeric mucin-like regions. the centr ... | 1996 | 8683213 |
| purified fusion protein vaccine protects against lower respiratory tract illness during respiratory syncytial virus season in children with cystic fibrosis. | to test in a double blind, placebo-controlled study a purified fusion protein (pfp-2) vaccine against respiratory syncytial virus (rsv) in rsv-seropositive children with cystic fibrosis (cf). | 1996 | 8684872 |
| [etiology of acute respiratory infections in 87 hospitalized children]. | the aim of this study was to assess the effectiveness of two microbiologic methods: viral culture and serology for the etiologic study of acute respiratory infections in children under 14 years of age admitted to our hospital. | 1996 | 8685493 |
| type-specific serologic diagnosis of respiratory syncytial virus infection, based on a synthetic peptide of the attachment protein g. | peptides deduced from the central hydrophobic region (residues 158-189) of the g protein of bovine and ovine respiratory syncytial virus (rsv) and of human rsv subtypes a and b were synthesized. these peptides were used to develop elisas to measure specifically antibodies against these types and subtypes of rsv. we have evaluated the bovine rsv-g peptide in both an indirect elisa and in a blocking elisa. specificity and sensitivity, relative to a routine diagnostic elisa that detects antibodies ... | 1996 | 8699029 |
| intranasal monoclonal iga antibody to respiratory syncytial virus protects rhesus monkeys against upper and lower respiratory tract infection. | respiratory syncytial virus (rsv), the major cause of lower respiratory tract disease in infants, is thought to infect the upper airways before spreading to the lower respiratory tract. a rhesus monkey model of rsv infection after upper airway inoculation was used to test the protective effect of intranasal treatment with hnk20, a mouse monoclonal iga antibody against rsv f glycoprotein. hnk20 was administered once daily for 2 days before rsv challenge and 4 days after challenge. treatment with ... | 1996 | 8699052 |
| induction of interleukin (il)-8 gene expression by respiratory syncytial virus involves activation of nuclear factor (nf)-kappa b and nf-il-6. | respiratory syncytial virus (rsv) preferentially infects respiratory epithelium and is an important cause of lower respiratory tract infections in young children. rsv induces the production of interleukin (il)-8 in airway epithelial cells; however, the mechanism of this induction is not known. to define the mechanism by which rsv induces il-8 gene activation, a549 epithelial cells were transfected with plasmids containing serial deletions of the 5'-flanking region of the il-8 gene and then expos ... | 1996 | 8699053 |
| discrimination of respiratory syncytial virus subgroups a and b by reverse transcription-pcr. | reverse transcription (rt)-pcr with shared primers differentiating respiratory syncytial virus (rsv) subgroups a and b was developed for subtyping of rsv isolates. results of rt-pcr were compared with those of an indirect immunofluorescence test using monoclonal antibodies. viral rna isolated from cell cultures infected with rsv served as a template for cdna synthesis with random primers. for pcr, we used three synthetic oligonucleotides corresponding to the g protein mrna sequence of subgroup a ... | 1996 | 8748269 |
| isolation of a neutralizing human rsv antibody from a dominant, non-neutralizing immune repertoire by epitope-blocked panning. | we isolated a large panel of human abs directed against the respiratory syncytial virus (rsv) ag from combinatorial phage display libraries. following initial differentiation of the fabs by bstni restriction patterns, dna sequence analysis revealed 10 different classes of vh paired with more than 35 different vl genes. all the fabs bound with high affinity to the f ag. however, most fabs competed with the binding of a representative member of this group, suggesting that the fabs recognized a com ... | 1996 | 8752928 |
| respiratory syncytial virus infection and meningococcal disease. | although viral respiratory tract infections may predispose to meningococcal disease, strong evidence that they do so exists only for influenza. data on laboratory reported cases of respiratory syncytial virus (rsv) infections and meningococcal disease in england and wales from mid-1989 to mid-1994 were analysed. although the rise in rsv cases preceded the rise in meningococcal disease cases each winter, the interval between the rise and fall of the two diseases was inconsistent, no association w ... | 1996 | 8760957 |
| rna replication by a respiratory syncytial virus rna analog does not obey the rule of six and retains a nonviral trinucleotide extension at the leader end. | genome analogs ("minigenomes") of sendai and measles viruses replicate efficiently only if their nucleotide length is an even multiple of six, a requirement called the rule of six (p. calain and l. roux, j. virol. 67:4822-4830, 1993; m. s. sidhu, j. chan, k. kaelin, p. spielhofer, f. radecke, h. schneider, m. masurekar, p. c. dowling, m. a. billeter, and s. a. udem, virology 208:800-807, 1995). the existence of a comparable requirement was tested for respiratory syncytial virus (rsv), which also ... | 1996 | 8764015 |
| fine mapping and characterization of the rous sarcoma virus pr76gag late assembly domain. | the p2 region of the rous sarcoma virus (rsv) gag polyprotein contains an assembly domain, which is required late in replication for efficient budding of virus-like particles from cells (j. w. wills, c. e. cameron, c. b. wilson, y. xiang, r. p. bennett, and j. leis, j. virol. 68:6605-6618, 1994). this domain, referred to as the l domain, was previously mapped to the 11 amino acids of p2b. through the analysis of a series of deletion and substitution mutations, the l domain has now been fine mapp ... | 1996 | 8764091 |
| respiratory syncytial virus-enriched globulin for the prevention of acute otitis media in high risk children. | acute otitis media (aom) has been associated with respiratory syncytial virus (rsv) infection; aom develops in up to one third of children with rsv illness. a masked multicenter trial used an immune globulin enriched with rsv-neutralizing antibodies (rsvig) to prevent rsv infection of the lower respiratory tract in 249 children with either bronchopulmonary dysplasia, congenital heart disease, or prematurity. to determine whether monthly rsvig therapy might decrease the incidence of aom, we retro ... | 1996 | 8765618 |
| respiratory syncytial virus bronchiolitis: clinical aspects and epidemiology. | sixty to ninety percent of the clinical syndrome of bronchiolitis is caused by respiratory syncytial virus (rsv) infection. rsv epidemiology has several unusual characteristics. rsv infects nearly all infants in the first year of life, with a peak incidence of hospitalized infants with bronchiolitis between 2-6 months of age. it is the only virus that causes most severe disease during the first month of life, i.e. at a time when maternal antibodies are present. lower respiratory tract infections ... | 1996 | 8766196 |
| respiratory syncytial virus is an important cause of community-acquired lower respiratory infection among hospitalized adults. | respiratory syncytial virus (rsv), the most important cause of lower respiratory disease in infants and young children, is rarely considered among the causes for community-acquired lower respiratory infection in adults. all noninstitutionalized adults hospitalized with community-acquired pneumonia in two ohio counties were evaluated between december 1990 and may 1992. fifty-three (4.4%) of 1195 adults admitted during the rsv seasons and 4 (1.0%) of 390 in the off-season had serologic evidence of ... | 1996 | 8769600 |
| amiloride inhibits cytokine production in epithelium infected with respiratory syncytial virus. | respiratory syncytial virus (rsv) is an important respiratory pathogen in infants and children. rsv preferentially infects airway epithelium and causes local production of inflammatory cytokines. ribavirin, the only specific agent available for treatment of rsv infection, has limited effectiveness. there are few data regarding the ability of drugs to modulate the inflammatory response of epithelium infected with rsv. this study evaluated the effect of amiloride and ribavirin on cytokine producti ... | 1996 | 8770057 |
| expression of a bifunctional chimeric protein a-vargula hilgendorfii luciferase in mammalian cells. | we have designed and constructed a novel chimeric protein that consisted of a single domain of protein a and luciferase derived from sea-firefly vargula hilgendorfii with the goal of obtaining a heterofunctional immunological tool. the structural gene of luciferase was fused to the 3' terminus of the d domain gene of protein a with/without a short linker of five amino acids. the resulting constructs under the transcriptional regulation of the rous sarcoma virus (rsv) promoter, were expressed tra ... | 1996 | 8770415 |
| selection and evolution of high-affinity human anti-viral antibodies. | high-affinity human anti-viral antibodies [e.g. for human immunodeficiency virus type 1 (hiv-1), respiratory syncytial virus (rsv) and herpes simplex virus (hsv)] can be selected from immune phage-display libraries using a variety of strategies. a small subset of these antibodies show potent neutralization in vitro and anti-viral efficacy in vivo in animal models. the affinities of such antibodies arising from secondary or higher order immune responses can be improved using "cdr walking'. sequen ... | 1996 | 8771795 |
| il-8 release from human neutrophils by the respiratory syncytial virus is independent of viral replication. | elevated interleukin-8 levels and a massive accumulation of neutrophils (pmn) are the hallmark of a variety of severe lung diseases. the respiratory syncytial virus (rsv), an important respiratory pathogen, induces interleukin-8 (il-8) release from human pmn, however, the mechanism is as yet unknown. we analyzed the role of virus uptake, intracellular virus replication, virus attachment, and of virus capsid proteins for the induction of il-8 (protein + mrna) in human pmn. cell supernatants were ... | 1996 | 8773587 |
| primary cytotoxic t-cell responses to bovine respiratory syncytial virus in calves. | bovine respiratory syncytial virus (rsv) is a major cause of respiratory disease in young calves. recent studies in calves, in which different t-cell subsets were depleted, have shown that cd8+ t cells play a central role in recovery from rsv infection. the present study demonstrates that rsv-specific, major histocompatibility complex-restricted cytotoxic t cells appear in the peripheral blood of gnotobiotic calves 7-10 days after infection with bovine rsv and were also detected in the lungs 10 ... | 1996 | 8774360 |
| invasive monitoring in infants with respiratory syncytial virus infection. | coincident with a change in the physician staff in our pediatric intensive care unit (picu), the frequency and duration of invasive monitoring were decreased. we examined the impact of this change on outcomes, complications, and hospital charges in infants admitted to the picu with respiratory syncytial virus (rsv) infection. | 1996 | 8774504 |
| effectiveness of ribavirin in otherwise well infants with respiratory syncytial virus-associated respiratory failure. pediatric critical study group. | to examine ribavirin's effectiveness in otherwise well infants with respiratory syncytial virus (rsv)-associated respiratory failure. | 1996 | 8774517 |
| ribavirin utilization and clinical effectiveness in children hospitalized with respiratory syncytial virus infection. | ribavirin was approved in early 1986 for treatment of illness associated with respiratory syncytial virus (rsv) infection in hospitalized children; however, the value of its use remains controversial. this investigation was undertaken to describe ribavirin utilization and to evaluate the effectiveness of ribavirin in reducing the period of hospitalization. all children with laboratory-confirmed rsv infection, hospitalized over seven epidemic periods at a single institution, were identified. char ... | 1996 | 8780603 |
| involvement of the complement system in the protection of mice from challenge with respiratory syncytial virus long strain following passive immunization with monoclonal antibody 18a2b2. | passive immunization of mice with 131 micrograms of the non-neutralizing monoclonal antibody (mab) 18a2b2, directed against the a subgroup epitope of the g glycoprotein of respiratory syncytial virus long strain (rsv), confers protection against viral i.n. challenge. the role of the fc fragment of this antibody as well as the involvement of antibody-dependent cellular cytotoxicity (adcc) and complement-mediated cytolysis towards protection was evaluated in vivo. passive immunization with the fab ... | 1996 | 8782350 |
| rapid diagnosis of respiratory syncytial virus infections in immunocompromised adults. | although rapid antigen detection methods for the documentation of respiratory syncytial virus (rsv) infections are widely used with pediatric patients, these tests have not been prospectively evaluated in immunocompromised (ic) adults. for bone marrow transplant recipients and adult patients undergoing chemotherapy for leukemia who had recent onset of respiratory symptoms, respiratory samples (combined nasal wash [nw]-throat swab [ts], endotracheal tube [et] aspirate, or bronchoalveolar lavage [ ... | 1996 | 8784563 |
| microwave-accelerated direct immunofluorescent staining for respiratory syncytial virus and influenza a virus. | a microwave-accelerated direct immunofluorescence staining method which requires only 20 min from specimen receipt to interpretation is as effective as conventional methods for detecting respiratory syncytial virus and influenza a virus antigens in clinical specimens. the time required compares favorably with that for the less sensitive abbott test pack rsv. | 1996 | 8784600 |
| dystrophin expression in muscles of mdx mice after adenovirus-mediated in vivo gene transfer. | we have generated high-titer adenoviral recombinants (avr) expressing a 6.3-kb partial dystrophin cdna insert under the control of either the rous sarcoma virus (rsv) or cytomegalovirus (cmv) promoter. these avr preparations were free of both e1-containing avr and avr with a nonfunctional dystrophin expression cassette. with these optimal avr preparations, we have obtained a high degree of short-term (10 days) expression of a truncated (approximately 200 kd) dystrophin in dystrophin-deficient md ... | 1996 | 8788164 |
| expression of full-length human dystrophin cdna in mdx mouse muscle by hvj-liposome injection. | duchenne muscular dystrophy (dmd) is an x-linked progressive muscle disorder which is caused by a defect of dystrophin, a 427-kda muscle cell membrane protein. one of the possible means of dmd therapy is to express the dystrophin gene in patients' muscles. in this study, full length dystrophin cdna was expressed in mdx (muscular dystrophy model) mouse muscle using the hemagglutinating virus of japan (hvj)-liposome method. with the hvj-liposome method, the lacz reporter genes were expressed in 50 ... | 1996 | 8789805 |
| an ultrastructural study of the interaction of human eosinophils with respiratory syncytial virus. | it was shown previously that eosinophils are activated in vivo and in vitro by respiratory syncytial virus (rsv) (garofalo et al., j pediatr 1992: 120: 28-32; kimpen et al., pediatr res 1992: 32: 160-4). for study of the interaction of eosinophils and rsv on the ultrastructural level, normodense eosinophils were purified from peripheral blood of healthy human volunteers. after incubation with rsv in the presence or absence of autologous serum, the eosinophils were examined with immunofluorescenc ... | 1996 | 8792384 |
| adenoviral-mediated thymidine kinase gene transfer into the primate brain followed by systemic ganciclovir: pathologic, radiologic, and molecular studies. | transduction of experimental gliomas with the herpes simplex virus thymidine kinase gene (hsv-tk) using a replication-defective adenoviral vector (adv/rsv-tk) confers sensitivity to ganciclovir (gcv) leading to tumor destruction and prolonged host survival in rodents. to determine treatment tolerance prior to clinical trials, we conducted toxicity studies in 6 adult baboons (papio sp.). the animals received intracerebral injections of either a high dose of adv/rsv-tk [1.5 x 10(9) plaque-forming ... | 1996 | 8793548 |
| adenovirus-mediated gene transfer of herpes simplex virus thymidine kinase in an ascites model of human breast cancer. | in this study, the growth of locally disseminated breast cancer was modeled using a human breast cancer cell line, mda-mb-435a, adapted to grow as an ascites tumor in athymic mice. ex vivo infection of mda-mb-435a cells with adenovirus containing the herpes simplex virus thymidine kinase gene (hsv-tk) were injected into the intraperitoneal cavity of athymic mice. ganciclovir (gcv) treatment resulted in prolonged median survival (117 vs. 34 days, p < 0.001) compared to untreated or control animal ... | 1996 | 8793549 |
| infection and replication of a planthopper transmitted virus-rice stripe virus in rice protoplasts. | rice stripe virus (rsv), a planthopper-transmitted virus, was inoculated into rice protoplasts, and a one-step growth curve was determined. the amount of virus in the protoplasts decreased following the inoculation, and then increased after 8 h. the replication of rsv reached its peak 20 h after inoculation. rsv replication in the inoculated protoplasts was further demonstrated by electron microscopy. | 1996 | 8793830 |
| recovery of infectious respiratory syncytial virus expressing an additional, foreign gene. | a previous report described the recovery from cdna of infectious recombinant respiratory syncytial virus (rsv) strain a2 (p. l. collins, m. g. hill, e. camargo, h. grosfeld, r. m. chanock, and b. r. murphy, proc. natl. acad. sci. usa, 92:11563-11567, 1995). here, the system was used to construct recombinant rsv containing an additional gene encoding chloramphenicol acetyltransferase (cat). the cat coding sequence was flanked by rsv-specific gene-start and gene-end motifs, the transcription signa ... | 1996 | 8794298 |
| effects of mutations in the gene-start and gene-end sequence motifs on transcription of monocistronic and dicistronic minigenomes of respiratory syncytial virus. | preceding and following each gene of respiratory syncytial virus (rsv) are two conserved sequences, the gene-start (gs) and gene-end (ge) motifs, respectively, which are thought to be transcription signals. the functions and boundaries of these signals and the process of sequential transcription were analyzed with cdna-encoded rna analogs (minigenomes) of nonsegmented negative-sense rsv genomic rna. two minigenomes were used. the monocistronic rsv-cat minigenome consists of the chloramphenicol a ... | 1996 | 8794332 |
| investigations of the japanese bovine tumour virus (blv)--its ability to express structural and regulatory blv proteins. | the mechanism of blv-induced tumorigenesis has not been clear up to now. changes of viral protein expression in infected cells may be involved in the molecular events leading to blv-induced leukaemogenesis. in this study western blot investigations of cells transfected with plasmid dna containing the complete japanese blv tumour clone provirus demonstrate that this provirus is unable to express gag and env proteins. following this an attempt was made to express the genes from this provirus in eu ... | 1996 | 8794696 |
| stimulation of mouse osteopontin promoter by v-src is mediated by a ccaat box-binding factor. | osteopontin is an arginine-glycine-aspartic acid-containing cell adhesion protein, which is frequently expressed in transformed cells and is thought to play a role in tumorigenesis. v-src is a transforming viral oncogene product encoded by rous sarcoma virus (rsv). we report that v-src expression in ht1080 fibrosarcoma cells significantly stimulates mouse osteopontin promoter activity. we also determined the v-src response element in the osteopontin promoter as an inverted ccaat box located at - ... | 1996 | 8798445 |
| prostate cancer gene therapy: herpes simplex virus thymidine kinase gene transduction followed by ganciclovir in mouse and human prostate cancer models. | prostate cancer is the most common internal malignancy in men in the united states. most cancers are diagnosed when they are locally advanced or metastatic and there is no effective treatment. in this study we evaluated the effectiveness of cytotoxic gene therapy in human pc-3 and du145 prostate cancer cell lines and in a rodent cell line, rm-1, derived from the mouse prostate reconstitution model system. the cell lines were efficiently transduced in vitro by a replicative-defective recombinant ... | 1996 | 8800746 |
| analysis of the human serological immune response to a variable region of the attachment (g) protein of respiratory syncytial virus during primary infection. | the serum antibody responses of babies to the variable carboxy-terminal region of the attachment (g) protein of respiratory syncytial virus (rsv) have been analysed using paired acute and convalescent sera from infants experiencing their first rsv infection with viruses of known genotype. the variable 84-85 carboxy-terminal amino acids of the g protein of six recent isolates of group a rsv were expressed in escherichia coli as fusion proteins with glutahione s-transferase. about half the infants ... | 1996 | 8801286 |
| pediatric investigators collaborative network on infections in canada (picnic) study of admission and management variation in patients hospitalized with respiratory syncytial viral lower respiratory tract infection. | to describe differences in patients hospitalized with respiratory syncytial virus (rsv) lower respiratory tract infection (lri) at nine canadian tertiary care hospitals. in addition, this study describes the variation in use of drug and other interventions. | 1996 | 8804328 |
| respiratory syncytial virus infection of human respiratory epithelial cells up-regulates class i mhc expression through the induction of ifn-beta and il-1 alpha. | cd8+ t cells mediate some of the damage to the lung epithelium following respiratory syncytial virus (rsv) infection. since cd8+ t cells recognize antigen-laden class i mhc molecules on the target cells, we examined in this study the expression of class i mhc by rsv-infected respiratory epithelial cells. respiratory epithelial cell lines and bronchial epithelial cells from normal human tissue responded to rsv infection with an increased expression of class i mhc as determined by flow cytometry a ... | 1996 | 8805651 |
| cloning of rous sarcoma virus enhancer factor genes. ii. rsv-ef-ii, abundantly expressed in fibroblasts and muscle tissue, binds to an octamer sequence, 5'-gtaccacc-3', in the noncoding strand of rsv enhancer. | rous sarcoma virus (rsv) mainly replicates in avian fibroblasts, and the u3 enhancer region of the long terminal repeats of rsv contains the determinants for its tissue-tropic expression. we describe the cloning and characterization of an avian gene that encodes a protein capable of binding to the enhancer region of rous sarcoma virus. a pcr-derived probe corresponding to the u3 region of rsv was used to isolate a cdna clone by screening a chicken cdna expression library. the cdna is predicted t ... | 1996 | 8806494 |
| immunotherapy of respiratory syncytial virus pneumonia following bone marrow transplantation. | respiratory syncytial virus (rsv) pneumonia is a well-recognized complication of bone marrow transplantation with a high mortality rate. we describe two patients who developed rsv pneumonia within the first 3 weeks following allogeneic bone marrow transplantation. these patients had significant oxygen requirements and radiographic infiltrates. both were treated with aerosolized ribavirin and given a single 1.5 gm/kg dose of intravenous immune globulin containing high levels of rsv neutralizing a ... | 1996 | 8807113 |
| prevalence of neutralizing antibody to respiratory syncytial virus in sera from mothers and newborns residing in the gambia and in the united states. | the prevalence of maternal respiratory syncytial virus (rsv)-neutralizing antibodies has been documented in developed countries, but there is little information from developing countries. we assessed the prevalence of rsv-neutralizing antibody in sera from gambian women and their newborns and compared them with their american counterparts during a similar period. the geometric mean titers of maternal antibodies to rsv subgroup a in the two populations were similar, while titers of antibodies to ... | 1996 | 8807217 |
| bronchial inflammation and the common cold: a comparison of atopic and non-atopic individuals. | cold virus infections are associated with asthma attacks and with increased bronchial responsiveness even in normal subjects. possible mechanisms include epithelial damage, interaction with adhesion molecules or with t-helper cell subsets. | 1996 | 8809424 |
| recombinant superoxide dismutase (sod) administered by aerosol inhibits respiratory syncytial virus infection in cotton rats. | recombinant (r) human (hu) manganese (mn) and copper-zinc (cuzn) superoxide dismutase (sod) were evaluated for their cytotoxicity and antiviral activity against respiratory syncytial virus (rsv) in tissue culture and in cotton rats. no apparent cytotoxicity or inhibition of rsv was observed in the tissue culture studies (both compounds had ic50 and ec50 values > or = 1000 micrograms/ml and a selective index = 1). however, significant reductions in mean pulmonary rsv titers (ranging between 0.5 a ... | 1996 | 8811202 |
| diagnosis of respiratory virus infections using gacelisa of urinary antibodies. | sensitive and specific methods are needed to diagnose respiratory virus infections using body fluids such as urine that, unlike blood samples, are readily obtained by non-invasive means. immunoglobulin g antibody capture enzyme-linked immunosorbent assays were developed for detection of antibody rises to respiratory syncytial virus and influenza a/taiwan (h1n1) after initial quantification and adjustment of urinary igg concentration. of 24 elderly subjects whose sera were assayed by the compleme ... | 1996 | 8814322 |
| a model for respiratory syncytial virus (rsv) infection based on experimental aerosol exposure with bovine rsv in calves. | five conventionally kept calves aged between 17 and 24 days were experimentally infected with bovine respiratory syncytial virus (brsv) by aerosol in order to mimic the natural infection route. the calves were killed and autopsies performed 7 days after the first virus challenge. the brsv isolate used induced tracheitis, bronchitis and atelectasis in infected calves. the only virus which could be isolated from the lungs of the calves was brsv. in addition, mycoplasma bovirhinis was isolated from ... | 1996 | 8814979 |
| immune responses of lambs to the fusion (f) glycoprotein of bovine respiratory syncytial virus expressed on insect cells infected with a recombinant baculovirus. | a group of lambs was immunized with the f protein of bovine respiratory syncytial virus expressed in a baculovirus (bac-f) and their humoral and cellular immune responses to bovine rsv studied before and after challenge with infectious bovine rsv. immunization with bac-f resulted in significant humoral immune responses as measured by virus neutralization and cellular immune responses as measured by lympho-proliferation against inactivated bovine rsv and specific cytotoxicity against autologous t ... | 1996 | 8817824 |
| respiratory syncytial virus infection enhances the response to laryngeal chemostimulation and inhibits arousal from sleep in young lambs. | to evaluate the effect of respiratory syncytial virus (rsv) infection on the response to laryngeal chemostimulation (lcs) with water, five lambs were inoculated with human rsv and three lambs were given control media at an age of 3-5 days. during rsv infection, lcs resulted in increased inhibition of minute ventilation and delayed recovery of regular breathing. sleep further increased the response, and arousal was less likely to occur in active sleep. two of the five infected lambs needed resusc ... | 1996 | 8819543 |
| reflex apnoea response and inflammatory mediators in infants with respiratory tract infection. | the reflex apnoea response to water stimulation was evaluated in infants with respiratory syncytial virus (rsv) infection and compared to the response in non-infected infants who had sustained an apparent life-threatening event (alte) or were siblings of infants who had died of sudden infant death syndrome (sids). rsv-infected infants had a significantly (p < 0.05) reinforced reflex apnoea response compared with non-infected infants. there was a significant negative correlation between the conce ... | 1996 | 8819544 |
| aetiology of pneumonia in hospitalized children. | one-hundred-and-thirty-two children with clinical and radiological evidence of bronchopneumonia/pneumonia were studied over a 1-year period for isolation/detection of bacterial and viral aetiological pathogens. throat swab, nasopharyngeal aspirate (npa), and lung aspirate were studied for bacterial and viral cultures. npa was also subjected to latex agglutination test (la) for h. influenzae and s. pneumoniae; and immunofluorescent technique (ifat) and enzyme immunoassay (eia) for respiratory syn ... | 1996 | 8820615 |
| [the standardization of an ultramicro elisa assay for the detection of igg antibodies to the respiratory syncytial virus]. | an ultramicro elisa assay of double antibody for the detection of igg antibodies to the respiratory syncytial virus (rsv) was standardized. it was used a rvs antiprotein f monoclonal antibody produced by the genetic engineering and biotechnology center (gebc) in havana. the use of this antibody allowed to include crude antigenic preparations instead of purified fractions, which caused a significant reduction of the reactivity obtained with the antigen control. the assay conditions were determine ... | 1996 | 9805041 |
| cloning, expression and functional activities of a single chain antibody fragment directed to fusion protein of respiratory syncytial virus. | hnk20 is a murine iga which is currently being investigated in clinical trials against respiratory syncytial virus (rsv) infections in infants and young children. | 1996 | 9373314 |
| [clinical, therapeutic and developmental aspects of acute bronchiolitis in tunisia]. | fourteen infants with severe acute bronchiolitis were admitted to the intensive care unit (icu) of tunis. this pathology represents 36% of severe bronchopulmonary infections admitted to this icu. their age ranged between 2 and 48 weeks (mean: 15 weeks). eight infants had hypotrophy. two infants had congenital heart disease and one infant had tracheo-bronchomalacia. viruses were found in 6/11 patients. respiratory syncytial virus (rsv) was identified in five patients and an adenovirus in one pati ... | 1996 | 9183423 |
| stable expression of anti-hpv 16 e7-ribozyme in cv-1 cell lines. | the hpv16 (human papilloma virus type 16) e7 gene product, an oncoprotein, has been considered to be involved in the pathogenesis of anogenital cancer, particularly of cervical cancer. in order to evaluate the effect of suppression of the expression of the e7 gene in cv-1 cells by ribozyme, rz523 with a transacting ribozyme targeted to the e7 rna and two processing ribozyme genes at the 5' and 3' flank was cloned into the eukaryotic expression plasmid prep9 under the control of rsv-ltr promoter. ... | 1996 | 9187492 |
| cell-mediated immune responses of lambs to experimental infection with bovine respiratory syncytial virus. | primary infection of lambs with bovine respiratory syncytial virus (rsv) was characterised by the presence of virus-specific cytotoxic cells in the peripheral blood as early as 5 days post-infection. these effector cells lysed virus-infected autologous targets in a self-restricted manner. depletion techniques revealed that cytotoxic activity was largely due to ovcd8+ cells. neutrophils obtained from experimentally infected lambs 5 to 10 days post-inoculation exhibited significant cytotoxic activ ... | 1996 | 9157688 |
| antigens of bovine respiratory syncytial virus in peripheral blood lymphocytes of experimentally infected lambs. | eight lambs were experimentally infected with bovine respiratory syncytial virus (rsv) and the distribution of viral antigen in lymphocyte subpopulations studied by flow cytometry. experimental infection with bovine rsv was characterised by significant changes in lymphocyte subpopulations. infection was followed by a significant (p <0.001) reduction in the number of cells expressing the ovcd5 epitope (t cells), due to a fall in the number of cells expressing the ovcd4 epitope (helper) and those ... | 1996 | 9157689 |
| respiratory syncytial virus infection in the elderly. | respiratory syncytial virus (rsv) is the most common cause of lower respiratory tract infections in infants and children throughout the world. respiratory syncytial virus infections in the elderly represent reinfections in the hosts who have had many prior episodes. thus, rsv infections are usually not considered serious in adults, since reinfections are generally known to result in mild disease. nevertheless, in adults, as in children, the infection has been reported to cause altered airway res ... | 1996 | 9072495 |
| human monoclonal fab fragments specific for viral antigens from combinatorial iga libraries. | iga constitutes the first line of immune defense, interacting with a variety of environmental antigens. following infection with respiratory syncytial virus (rsv) individuals frequently exhibit elevated serum iga titers specific for the virus. previously combinatorial igg libraries have successfully been used to clone such human antibody responses. | 1995 | 9373330 |
| respiratory syncytial virus infection: clinical presentation and management. | rsv infection continues to be a major cause of morbidity and mortality throughout the world. despite advances in the understanding of its pathogenesis, limited progress has been made in prevention and treatment of rsv infection. based on the experiences thus far it seems that control of rsv infection will be a difficult and complex task. | 1995 | 10829841 |
| physical properties of aerosolized immunoglobulin for inhalation therapy. | animal studies suggest that passive immunization by intratracheal application of gammaglobulins may succeed in treating small children and infants with severe lower respiratory tract infection caused by respiratory syncytial virus (rsv). since proteins foam easily when stirred up or used with compressed air, the feasibility of nebulization of the immunoglobulin solution (ivig, sandoglobulin) had to be tested. the therapeutic effect of a drug delivered via inhalation is proportional to the mass o ... | 1995 | 10155651 |
| production of defective virus by terminally differentiated myotubes infected with rous sarcoma virus. | the generally accepted concept that the replication of rous sarcoma virus (rsv) is dependent on host cell dna synthesis was reexamined. as the host we used terminally differentiated myotubes (mt), in which no cellular dna synthesis is observed. as an extension of our previous study which indicated that rsv-infected mt produce various virus components, we examined viral particles produced by infected mt. electron microscopy showed presence of viral particles released from infected mt. immunopreci ... | 1995 | 8825300 |
| approaching the rsv season with a nursing plan of action. | as the statistics show with year-in, year-out regularity, during november through march in the united states, approximately 90,000 infants and young children will be hospitalized with a severe lower respiratory infection attributable to the respiratory syncytial virus (rsv). this virus, discovered only as recently as 1956, appears to be ubiquitous, infecting virtually 100% of children by age 4. for most of them the resulting illness will be mild and easily vanquished by an intact immune system. ... | 1995 | 8700601 |
| effect of orthovanadate on commitment of avian myoblasts transformed with rous sarcoma virus to myogenic differentiation. | myogenic differentiation of quail myoblasts transformed with a temperature-sensitive mutant of rous sarcoma virus (qm-rsv cells) depends on the temperature: at 35.5 degrees c, the permissive temperature for the virus, the transformed myoblasts proliferate, without fusion, but at 41 degrees c, the nonpermissive temperature, they become committed to myogenic differentiation until about 10 h and then myoblast fusion occurs within 24 h. this temperature dependency of the differentiation reaction is ... | 1995 | 8603684 |
| detection of respiratory syncytial virus by reverse transcription-pcr and hybridization with a dna enzyme immunoassay. | nasal aspirates from 238 infants hospitalized with acute respiratory infections during the winter of 1994 and 1995 were tested for respiratory syncytial virus (rsv) by immunofluorescence assay (ifa) and the viral isolation technique (vit) and by two pcr and hybridization methods: reverse transcription pcr 1 (rt-pcr1), which amplifies the rnas of all rsv strains, and rt-pcr-2, which allows subgroup classification of rsv. rt-pcr-1 and rt-pcr-2 detected viral sequences in 56.7% (135 of 238) and 48. ... | 1995 | 8586738 |
| early-onset respiratory syncytial virus pneumonia after allogeneic bone marrow transplantation. | a 41-year-old woman with follicular lymphoma developed sporadic respiratory syncytial virus (rsv) pneumonia shortly after allogeneic bone marrow transplantation from an hla-matched sibling. rsv pneumonia is potentially fatal in immunosuppressed patients, but she improved along with the recovery of bone marrow function without specific treatment. early recovery of myelosuppression supported by granulocyte colony-stimulating factor may have helped to control rsv pneumonia. | 1995 | 8589364 |
| activation of homologous complement via the alternative complement pathway by quail cells transformed by rous sarcoma virus. | after incubation of the cells with fresh quail serum, deposition of the third component of complement (c3) was demonstrated on the cell surface of various quail cell lines transformed by rous sarcoma virus (rsv) as well as on that of primary quail embryo (qe) cells transformed by rsv. the c3 deposition occurred irrespective of virus production. on the other hand, the c-3 deposition was not observed on two quail cell lines transformed by a chemical carcinogen, qe cells infected with avian leukosi ... | 1995 | 8593301 |
| respiratory syncytial virus and parainfluenza virus infections in the immunocompromised host. | respiratory syncytial virus (rsv) and parainfluenza virus (piv) are common causes of respiratory infections in immunocompetent children under the age of 6 years. these viruses belong to a family of enveloped single-stranded rna viruses, the paramyxoviruses. the clinical manifestations in the normal host range from mild illness to severe croup, bronchiolitis, and pneumonia. after the age of 6 years, reinfections occur, but are characterized by diminishing frequency and severity. in contrast to th ... | 1995 | 8668850 |
| evaluation of the protective efficacy of reshaped human monoclonal antibody rshz19 against respiratory syncytial virus in cotton rats. | reshaped human mab rshz19, which is specific for the surface fusion protein of respiratory syncytial virus (rsv) is in clinical development for the prevention and treatment of rsv-induced disease in human infants. the current studies profile lung virus clearance and evaluate lung histopathology in mab-treated, rsv-infected cotton rats, a well characterized model of rsv infection. the highest dose of this mab (10 mg/kg) administered parenterally 24 h before infection decreased subgroup a or b rsv ... | 1995 | 8559607 |
| respiratory syncytial virus pneumonia in hospitalized adult patients with leukemia. | respiratory syncytial virus (rsv) has been demonstrated to be an important cause of life-threatening pneumonia in adult bone marrow transplant recipients; however, its role in other immunocompromised adults has not been defined. we prospectively studied all adult patients with leukemia who were hospitalized at m. d. anderson cancer center (houston) during a 1-year period (november 1993 through october 1994). during a 19-week period when rsv was prevalent in the community, it was isolated from 9 ... | 1995 | 8562747 |
| lobar emphysema associated with respiratory syncytial virus pneumonia. | the etiology of infantile lobar emphysema is frequently unknown; however, partial airway obstruction is most often implicated. although infection and lobar emphysema have been linked previously, their cause-and-effect relationship is unclear. we present the clinical, radiographic, and pathologic findings in a 9-day-old infant with acute bronchiolitis due to respiratory syncytial virus (rsv) and a clinical and radiographic appearance suggestive of lobar emphysema. pathologically, there was diffus ... | 1995 | 8570321 |
| respiratory syncytial virus increases il-8 gene expression and protein release in a549 cells. | the mechanism of respiratory syncytial virus (rsv)-induced inflammation in the airways of infants and children is not fully understood. we hypothesized that rsv directly induces interleukin (il)-8 gene expression in airway epithelial cells, independent of il-1 beta and tumor necrosis factor-alpha (tnf-alpha) production. exposure of a549 cells (an airway epithelial cell line) to rsv resulted in increased il-8 mrna expression and il-8 protein release from the cells as early as 2 h after treatment. ... | 1995 | 8572249 |
| suppression of activation of the human immunodeficiency virus long terminal repeat by cd8+ t cells is not lentivirus specific. | cd8+ t lymphocytes of hiv-1-infected individuals can efficiently suppress hiv-1 replication in cd4+ t lymphocytes. to elucidate the molecular events underlying this suppression, we have used the hiv-1 ltr directing the chloramphenicol acetyltransferase gene (cat) in transient transfection assays using human jurkat t cells. in addition to supernatants of patient cd8+ t lymphocytes (cd4+ > 350/microliters), supernatant of a t cell clone derived by herpesvirus saimiri (hvs)-mediated transformation ... | 1995 | 8573388 |
| effects of some inhibitors on myogenic differentiation of avian myoblasts transformed with rous sarcoma virus. | the differentiation of quail myoblasts transformed with a temperature-sensitive mutant of rous sarcoma virus (qm-rsv cells) depends on culture temperature. at 35.5 degrees c, the permissive temperature for rsv, qm-rsv cells repeatedly proliferate without differentiation. however, myogenic differentiation proceeds at 41 degrees c, the nonpermissive temperature for rsv. to obtain useful inhibitors for the analysis of myogenic differentiation in qm-rsv cells, various drugs of myoblast fusion report ... | 1995 | 8581993 |
| the clinical picture presented by premature neonates infected with the respiratory syncytial virus. | during the course of a 3-year epidemic of respiratory syncytial virus (rsv) in an neonatal intensive care unit, we examined premature neonates for the clinical features of rsv infections and the risk factors predisposing towards transmission of the disease. seventy-nine infants with a median gestational age of 31 weeks were tested in 113 episodes of clinical deterioration for the presence of rsv antigen in nasopharyngeal secretions. forty-seven results were positive and 66 negative. bradycardia, ... | 1995 | 8582403 |
| isolation of virus-neutralizing rnas from a large pool of random sequences. | rna and ribonuclease-resistant rna analogs that bound and neutralized rous sarcoma virus (rsv) were isolated from a large pool of random sequences by multiple cycles of in vitro selection using infectious viral particles. the selected rna pool of rsv-binding sequences at a concentration of 0.16 microm completely neutralized the virus. of 19 sequences cloned from the selected pool, 5 inhibited rsv infection. the selected rna and rna analogs were shown to neutralize rsv by interacting with the vir ... | 1995 | 8524793 |
| production of infectious human respiratory syncytial virus from cloned cdna confirms an essential role for the transcription elongation factor from the 5' proximal open reading frame of the m2 mrna in gene expression and provides a capability for vaccine development. | infectious human respiratory syncytial virus (rsv) was produced by the intracellular coexpression of five plasmid-borne cdnas. one cdna encoded a complete positive-sense version of the rsv genome (corresponding to the replicative intermediate rna or antigenome), and each of the other four encoded a separate rsv protein, namely, the major nucleocapsid n protein, the nucleocapsid p phosphoprotein, the major polymerase l protein, or the protein from the 5' proximal open reading frame of the m2 mrna ... | 1995 | 8524804 |
| a plasmid-based self-amplifying sindbis virus vector. | sindbis virus was used as a self-amplifying eukaryotic expression vector. a recombinant cdna genome of this (+)-strand rna virus was placed under the transcriptional control of a rous sarcoma virus ltr (rsv) promoter. transfection of this plasmid construct into mammalian cell lines (3t3, hepg2, and 293 cells) resulted in expression of the luciferase reporter gene. high-expression levels were also measured after transfection into primary rat myoblasts. in differentiated myotubes, expression level ... | 1995 | 8527474 |
| combination therapy with aerosolized ribavirin and intravenous immunoglobulin for respiratory syncytial virus disease in adult bone marrow transplant recipients. | respiratory syncytial virus (rsv) infections in adult bmt recipients are frequently complicated by fatal pneumonias. therapy of rsv pneumonia with aerosolized ribavirin alone has been reported to be associated with a 70% mortality rate. because immune globulin therapy has been reported to be beneficial, we conducted a prospective trial of combination therapy with aerosolized ribavirin and intravenous immunoglobulin (ivig). aerosolized ribavirin was administered at 20 mg/ml for 18 h a day and ivi ... | 1995 | 8535312 |
| inhibitory effect of pyridobenzazoles on virus replication in vitro. | twelve species of pyridobenzazoles and pyrimidobenzimidazole were examined as inhibitors of the replication of respiratory syncytial virus (rsv) in hela cells. from the pyridobenzazoles studied, 2-benzamido-4-cyano-1-oxo-1h,5h-pyrido[1,2-alpha]benzimidazol emerged as a potent inhibitor of the rsv. based on its inhibitory effect on the cytopathogenicity of the rsv in hela cells, the 50% effective dose was found to be 0.95 micrograms/ml. the cytotoxicity of this compound for hela cells was examine ... | 1995 | 8535400 |
| vaccination with a low-oncogenic strain of rous sarcoma virus prevents visceral tumors in chickens. | the oncogenic potential of different strains of rous sarcoma virus (rsv) varies significantly in two mhc(b) congenic chicken lines cb and cc and in their f1 hybrids with the unrelated inbred line ia. the bryan high-titer pseudotype of rsv of antigenic subgroups d and b (bh-d, and bh-b) was highly oncogenic, eliciting mostly fatal, progressively growing tumors. visceral tumor formation and erythroblastosis were seen after challenge with bh-b in cb and cc chickens, but not in their f1 hybrids with ... | 1995 | 8919361 |
| efficient expression of pro-urokinase by human lymphoblastoid namalwa kjm-1 cells using moloney retroviral promoter. | we have compared the level of expression of several enhancer/promoters in human lymphoblastoid namalwa kjm-1 cells when fused to a common reporter gene. a cassette containing the pro-urokinase (pro-uk) coding sequence followed by the rabbit beta-globin and simian virus 40 (sv40) 3' nontranslated region was used for evaluation of the enhancer activity. cells containing moloney murine leukemia virus (mo-mulv) promoter had an average of 10-20 fold higher expression levels of pro-uk than those conta ... | 1995 | 8920107 |
| a colorimetric ldh assay for the titration of infectivity and the evaluation of anti-viral activity against ortho- and paramyxoviruses. | a rapid and precise screening assay was developed for in vitro evaluation of anti-orthomyxo- and anti-paramyxovirus agents. the procedure is spectrophotometrical assessment for viability of cells via extracellular leakage of lactic dehydrogenase (ldh). hmv-ii cells, a human melanoma cell line was found to be suitable for the titration of virus infectivity and screening of anti-viral agents for orthomyxo- and paramyxoviruses. comparative titration of infectivity of stock viruses by the ldh and th ... | 1995 | 8928191 |
| evaluation of subgroup-specific peptides of the g protein of respiratory syncytial virus for characterization of the immune response. | two synthetic peptides, designated peptides 12g(a) and 12g(b), representing amino acids 174-188 of the g glycoprotein of respiratory syncytial virus (rsv) subgroup a (strain a2) and subgroup b (strain ch18537) were evaluated for their properties as subgroup-specific antigens for enzyme immunoassay (elisa). these peptides were used to characterize the immune response of children with naturally occurring rsv infection during six annual epidemics in the huntington area, west virginia, usa; viz. 197 ... | 1995 | 8830114 |
| evidence that the amino acid region 124-203 of glycoprotein g from the respiratory syncytial virus (rsv) constitutes a major part of the polypeptide domain that is involved in the protection against rsv infection. | the first 230 residues of the 298-amino acid glycoprotein g of respiratory syncytial virus (rsv) are sufficient to confer complete resistance to challenge with live rsv, whereas the first 180 residues completely failed (olmsted et al. (1989) j. virol. 63, 411-420). the characterization of a protective epitope corresponding to the amino acid region 174-187 of the g protein (trudel et al. (1991) virology 185, 749-757) suggests that interruption of this region in the 180 residue truncated polypepti ... | 1995 | 8669890 |
| orthomyxoviral and paramyxoviral infections in transplant patients. | in summary, orthomyxo- and paramyxoviruses cause clinically important infections in transplant patients. patients often develop lower respiratory tract involvement and sometimes respiratory failure, which almost is uniformly fatal. bone marrow transplant recipients appear to be at higher risk of severe disease than are solid-organ recipients, but well defined criteria to predict those patients who will be severely affected are not available. factors associated with more severe disease include th ... | 1995 | 8747776 |
| adenovirus-mediated expression of a reporter gene in thalamocortical cocultures. | organotypic cocultures of thalamic and cortical explants have recently been used to study the development of the thalamocortical axonal network in the mammalian neocortex. to explore the possibility of genetically manipulating organotypic explants, rat thalamocortical (tc) cocultures were infected with the recombinant adenovirus, adv/rsv beta gal. infection of the cortical explants resulted in long-term expression (2 weeks) of the reporter gene (beta-galactosidase) with no significant alteration ... | 1995 | 8719624 |
| activation of complement in quails bearing rous sarcoma virus-induced tumors. | the concentration of serum c3 in quails bearing tumors induced by rous sarcoma virus (rsv) was elevated in parallel with tumor growth, whereas serum c3 levels in quails inoculated with avian leukosis virus, which lacks transforming activity, showed a pattern similar to that in mock-infected quails. c3 deposition was also observed in almost all tumor cells at the tumor developing stage. these findings obtained in vivo suggest that the cells transformed by rsv activated the c3 on their surfaces. | 1995 | 8720051 |
| expression of the herpes simplex virus type 1 glycoprotein e in human cells and in escherichia coli: protection studies against lethal viral infection in mice. | the objective of this study was to examine the protective efficacy of purified recombinant herpes simplex virus type 1 (hsv-1) glycoprotein e (ge-1) in the mouse lethal challenge model. a secreted form of ge-1 (hge-1s) protein, containing amino acids 1-406, was produced in human cells by using the episomal replicating prp-rsv expression vector. in addition, a portion of the ge-1 (bge-1t) protein corresponding to amino acids 90-406, was expressed in escherichia coli as a fusion protein with malto ... | 1995 | 8847521 |
| synthesis and antiviral activities of 9-cyclopentylpurine derivatives. | the synthesis and anti-rsv (rouse sarcoma virus) activity of hmca, (+/-)-9-(2-hydroxymethyl-cyclopenthyl)-adenine, and its derivatives are described. it has been demonstrated that trans-hmca has greater anti-rsv activity in tissue culture than cis-hmca. | 1995 | 8841575 |
| detection of respiratory syncytial virus from clinical specimens: comparison between reverse transcription polymerase chain reaction and tissue culture. | rt-pcr was compared with tissue culture to detect rsv from nasopharyngeal aspirates. rt-pcr was more sensitive and specific than tissue culture method. rt-pcr has sensitivity and specificity of 100% and 97%, respectively. the results indicate that rt-pcr can be used for detection of rsv in clinical specimens. | 1995 | 9139376 |