Publications

a two-year study of enteric infections associated with diarrhoeal diseases in children in urban somalia.	a hospital-based systematic sample of 1667 children with severe diarrhoeal disease was studied in mogadishu, somalia, throughout 1983 and 1984. one or more enteric pathogens were found in 61% of the patients. rotavirus (25%), enterotoxigenic escherichia coli (11%), shigella spp. (9%), aeromonas hydrophila (9%), giardia lamblia trophozoites (8%), campylobacter jejuni (8%), and vibrio cholerae non-o1 (6%) were the most frequently identified pathogens. age-specific detection rates of enteric pathog ...	1988	3076719
epidemiology of aeromonas and plesiomonas diarrhoea.		1988	3077628
comparison of methods for the induction, propagation and recovery of l-phase variants of aeromonas spp.		1988	3077629
aeromonas and plesiomonas in the environment: value of differential biotyping of aeromonads.		1988	3077630
human extraintestinal infections caused by aeromonas species.		1988	3077631
drug-resistant plasmids from fish pathogens.	the epidemiological surveillance of drug-resistant strains of the fish pathogenic bacteria vibrio anguillarum and pasteurella piscicida carrying transferable r plasmids in fish farms is described. the dna structure of r plasmids, and the drug-resistant determinants of r plasmids from the fish pathogens aeromonas hydrophila,aeromonas salmonicida, edwardsiella tarda, v. anguillarum, and p. piscicida are discussed.	1988	3079184
pathogenicity of aeromonas sp. for balb/c mice.		1988	3130510
purification and characterization of salmolysin, an extracellular hemolytic toxin from aeromonas salmonicida.	an extracellular hemolytic toxin of aeromonas salmonicida, termed salmolysin, was purified 945-fold by ammonium sulfate precipitation, anion-exchange chromatography on deae-cellulose, and gel filtration chromatography on sephadex g-100 and sepharose 2b. salmolysin appeared homogeneous upon cellulose acetate membrane electrophoresis and immunodiffusion analysis. the molecular weight of the toxin was estimated to be approximately 200,000 by the sedimentation equilibrium method. the uv absorption s ...	1988	3136147
wound infection with aerogenic aeromonas strains: a review of twenty-seven cases.	aeromonas spp. have been implicated in a wide spectrum of human disease including wound infection. a review is made of local experience of 27 wound infections from which aerogenic strains of aeromonas were recovered; of these, aeromonas was isolated in pure culture from five infections. twenty-five infections were related to trauma and were community acquired, while two were probably nosocomial. of the 25 community acquired infections, 16 were associated with contamination by soil and one with w ...	1988	3137035
[development of aeromonas hydrophila septicemia in a young healthy woman].		1988	3138333
acute diarrhea in children in shanghai.		1988	3138335
aeromonas schubertii, a new mannitol-negative species found in human clinical specimens.	in 1983 the vernacular name enteric group 501 was coined for a group of strains that had been referred to our laboratory as "possible vibrio damsela that does not require nacl for growth." by dna-dna hybridization (hydroxyapatite method, 32p, 60 and 75 degrees c), six strains of enteric group 501 were closely related to the labeled strain 2446-81 (70 to 95% at 60 degrees c and 71 to 93% at 75 degrees c; 0 to 1% divergence). type strains of all aeromonas species and reference strains of six other ...	1988	3139706
[disease of the fins of rhamdia sapo. isolation of the etiological agents and experimental infection].	during intensive culture of south-american catfish, rhamdia sapo fingerlings an epizootic similar to fin rot was developed. aeromonas hydrophila and pseudomonas aeruginosa were isolated and they were treated and controlled with daily short dip, 1 ppm furanace. the disease was experimentally reproduced and we concluded that it is result of interaction of unfavorable environment conditions such as: low dissolved oxygen, increase of solids in suspension; host condition and the presence of aeromonas ...	1988	3140294
aeromonas species in a municipal water supply of a central european city: biotyping of strains and detection of toxins.	in the course of a one-year study, 78 out of 239 samples from a central european municipal water supply were found to contain aeromonas species. biotyping and hemolysin titers according to the criteria of burke et al. (1) showed that almost 50% of the tested strains can be presumed to be enterotoxin-producing. the remaining microbiological parameters met international standards (20).	1988	3140537
evaluation of 9-chloro-9-[4-(diethylamino)phenyl]- 9,10-dihydro-10-phenylacridine hydrochloride (c-390) in broth and agar media for identification of pseudomonas aeruginosa.	brain heart infusion broth and mueller-hinton agar containing the compound 9-chloro-9-[4-(diethylamino)phenyl]- 9,10-dihydro-10-phenylacridine hydrochloride (c-390) were evaluated as selective media for identifying pseudomonas aeruginosa. of the 192 pseudomonas spp. and 68 additional oxidase-positive or glucose-nonfermenting gram-negative organisms tested, only p. aeruginosa (120 of 121 isolates) and certain strains of pseudomonas cepacia, aeromonas hydrophila, and achromobacter xylosoxidans wer ...	1988	3141473
experimental evidence for enteropathogenicity in aeromonas veronii.	eleven ornithine-positive strains of aeromonas (9 a. veronii and 2 provisionally classified as aeromonas species ornithine positive) were tested for ability to cause fluid accumulation in the rabbit ileal loop. all eight beta-hemolytic strains caused fluid accumulation. gel diffusion analysis revealed that the a. veronii beta-hemolysin was serologically related to the a. hydrophila beta-hemolysin, a known enterotoxic molecule. the biological activity of the a. veronii hemolysin was neutralized b ...	1988	3143475
comparative in vitro activity of the new difluoro-quinolone temafloxacin (a-62254) against bacterial isolates from cancer patients.	the in vitro activity of temafloxacin, a new difluoro quinolone agent, against 725 bacterial isolates representing 32 species was evaluated in comparison with that of ciprofloxacin. temafloxacin inhibited the majority of enterobacteriaceae isolates at a concentration of less than or equal to 0.5 microgram/ml. it was also extremely active against acinetobacter spp. and aeromonas hydrophila. its activity was 2-8 fold less than that of ciprofloxacin against most gram-negative isolates. methicillin- ...	1988	3143585
bacterial hemolysins as virulence factors.		1988	3144241
[evaluation of 2 culture media for the isolation and enumeration of motile aeromonas in different kinds of water].	in recent years an increasing incidence of aeromonas-related cutaneous infections and gastroenteritis has raised a serious public health problem. it appeared therefore timely to define a specific method allowing the rapid isolation and enumeration of the bacteria in their various aquatic habitats. in this line of research we have compared the growth of aeromonas originating from different aquatic sources and raised on two media, i.e. rs-agar and pxa-agar. whatever the aquatic system we observed ...	1988	3144424
properties of aeromonads and their occurrence and hygienic significance in drinking water.	in the netherlands, aeromonads in drinking water have attracted much attention in recent years. this development was caused by a sudden increase of the aeromonas density in the drinking water of the municipal dune waterworks of the hague and reports about the possible health significance of these organisms in drinking water. literature data indicate that representatives of the motile aeromonas species a. hydrophila, a. sobria and a. caviae generally have been observed in larger percentages of di ...	1988	3146172
aeromonas hydrophila infection after cockles ingestion.		1988	3148523
in vitro activity of fleroxacin (ro23-6240), a new fluorinated 4-quinolone against isolates from cancer patients.	the in vitro activity of fleroxacin (ro23-6240; am 833), a new fluorinated 4-quinolone, was compared to that of ciprofloxacin, enoxacin and a-56620, against 747 isolates from cancer patients. fleroxacin inhibited more than 90% of enterobacteriacea isolates at a concentration of less than or equal to 0.25 micrograms/ml. it was also extremely active against aeromonas hydrophila and haemophilus influenzae isolates with mic90 values of 0.12 and 0.06 micrograms/ml, respectively. the mic90 for acineto ...	1988	3149568
critical ionizing groups in aeromonas neutral protease.	aeromonas neutral protease possesses two residues critical to its activity. one has a pka of 5.5 in both the free enzyme and the enzyme-substrate complex and must be deprotonated for maximal activity. the other, which ionizes at ph 7.1 in the free enzyme and at ph 7.4 in the enzyme-substrate complex, must be protonated for optimal enzyme action. the protease is reversibly inhibited by aminoacyl hydroxamates, peptides containing a phenylalanyl residue, phosphoryl-l-phenylalanylglycylglycine, and ...	1988	3123480
effects of the antimicrobic tiamulin on seven gram-negative bacterial fish pathogens.	in vitro and in vivo test were carried out with tiamulin and gram-negative bacterial pathogens of fish. determination of minimum inhibitory concentration for 51 strains of seven species of gram-negative bacterial pathogens showed that only strains of vibrio anguillarum were sensitive at 1.6-6.25 ppm, while the rest of test strains required 25- greater than 100 ppm. control of infection was not achieved when tiamulin was fed for 14 days at 5 or 50 mg/kg to rainbow trout (salmo gairdneri) experime ...	1988	3127604
aeromonas-associated gastroenteritis in children.	during a 20-month period 55 strains of aeromonas species were isolated from 53 children with diarrhea. the isolation rate of 2.5% for aeromonas compared with the rates of 4.5% for shigella, 3.3% for salmonella, 2.7% for campylobacter and 0.05% for yersinia. in 45 children aeromonas was the sole bacterial enteropathogen identified. aeromonas was also isolated from 2 (0.5%) of 380 asymptomatic children. despite its known lack of identifiable virulence properties, aeromonas caviae was the most prev ...	1988	3340460
aeromonas hydrophila wound infection in a patient with cyclic neutropenia following a piranha bite.		1988	3340462
effect of incubation temperature on growth and soluble protein profiles of motile aeromonas strains.	motile aeromonas spp. are said to have an optimal growth temperature of 28 degrees c. we performed growth studies on 24 isolates at 29 and 37 degrees c and found that the optimal growth temperature was not necessarily 28 degrees c. we also detected temperature-dependent differences in soluble protein production in some of these strains.	1988	3343335
production of monoclonal antibody against aeromonas hydrophila haemolysin.	two hybridoma cell lines that produce monoclonal antibodies against aeromonas hydrophila haemolysin were established by fusion of myeloma and spleen cells obtained from a mouse immunised with haemolysin detoxified with tetranitromethane. enzyme-linked immunosorbent assay (elisa) showed that the two purified monoclonal antibodies, b7 and b11, recognised the same epitope on the haemolysin molecule. antibody b7 neutralised the haemolytic and enterotoxic activities of the haemolysin. it is concluded ...	1988	3346901
[prevalence of aeromonas spp. pediatric gastroenteritis].	a selective medium and biochemical tests were used to search for aeromonas spp. in the stools of 536 children, more than 90% of whom had "gastroenteritis", seen at sainte-justine hospital, montreal, in june or july 1986. the organism was found in seven children, six of whom had bloody diarrhea. one of the six, a 6 1/2-year-old boy, required intravenous alimentation and later manifested ulcerative colitis; the other five recovered. the remaining child, a 14-year-old boy, had synovitis of the knee ...	1988	3355951
gas-liquid chromatographic analysis of fatty acid methyl esters of aeromonas hydrophila, aeromonas sobria, and aeromonas caviae.	clinical isolates of aeromonas hydrophila, a. sobria, and a. caviae whose fatty acid content had been analyzed by gas-liquid chromatography (glc) displayed the following qualitatively similar glc profiles: 12:0, 14:0, 15:0, 16:0, 17:0, 18:0, 16:1, 18:1, a-15:0, a-17:0, and 3-oh 14:0. the 16:0/17:0 area-percentage ratio separated the clinical aeromonads in accordance with their species designations. aeromonads treated with subminimal inhibitory concentrations of the antibiotic cerulenin displayed ...	1988	3366862
structure of an s layer on a pathogenic strain of aeromonas hydrophila.	negative staining revealed a tetragonal surface array (s layer) on all the members of a serogroup of aeromonas hydrophila which possess high virulence for fish. the s layers were similar on all the strains examined, with unit cell dimensions of approximately 12 nm. a single representative strain, strain tf7, was selected for further analysis. freeze-cleaved and etched preparations and sections for electron microscopy showed that the s layer was the outermost component of the cell envelope. this ...	1988	3372478
isolation and biochemical characterization of the s-layer protein from a pathogenic aeromonas hydrophila strain.	the regular surface protein array (s layer) present on aeromonas hydrophila tf7 is composed of a single species of protein of apparent molecular weight 52,000. this protein was extracted from whole cells by treatment with 0.2 m glycine hydrochloride (ph 3.0). the protein was purified to homogeneity by ion-exchange chromatography and reverse-phase high-performance liquid chromatography. amino acid composition analysis showed that the protein contained 520 residues per molecule, 41% of which were ...	1988	3372479
isolation of aeromonas salmonicida from masu salmon in the republic of korea.	the isolation and identification of the gram-negative fish pathogen, aeromonas salmonicida salmonicida, from masu salmon (oncorhynchus masou) at the yang yang salmon hatchery in the republic of korea is described. the bacterium possessed certain characteristics in common with isolates from fish in japan. it is an important cause of mortality among cultured salmonids in the republic of korea. this is the first report of this fish pathogen from the asian mainland.	1988	3373645
preparation of metal-free enzymes.		1988	3374375
screening of bacteria with antiviral activity from fresh water salmonid hatcheries.	bacteria isolated from two salmonid hatcheries were screened for antiviral activity against infectious hematopoietic necrosis virus (ihnv) to ascertain the presence of bacteria with anti-ihnv activity in the aquatic environment. out of 710 bacterial isolates from the water and sediment samples, 190 strains showed anti-ihnv activities of more than 50% plaque reduction. these antiviral activities were detected predominantly in pseudomonas, aeromonas/vibrio, and coryneforms. in one hatchery, the ba ...	1988	3374405
an unusual case of pyogenic meningitis due to aeromonas sobria.		1988	3382842
aeromonas hydrophila cellulitis. a case report.		1988	3385616
the phylogenetic origin of the bifunctional tyrosine-pathway protein in the enteric lineage of bacteria.	because bifunctional enzymes are distinctive and highly conserved products of relatively infrequent gene-fusion events, they are particularly useful markers to identify clusters of organisms at different hierarchical levels of a phylogenetic tree. within the subdivision of gram-negative bacteria known as superfamily b, there are two distinctive types of tyrosine-pathway dehydrogenases: (1) a broad-specificity dehydrogenase (recently termed cyclohexadienyl dehydrogenase [cdh]) that can utilize ei ...	1988	3386529
the effects of gamma irradiation on the lymphoid organs of rainbow trout and subsequent susceptibility to fish pathogens.	fish were irradiated with 60co gamma rays at doses ranging from 10 to 50 gy. lethal doses were determined in fishes of different ages. for a given dose, fry and fingerlings were more susceptible than subadults. whatever the irradiation dose was, the fish displayed a sharp decrease in blood leucocyte count. at the lowest doses, this acute leucopaenia was reversible. the cellular damage in the lymphoid organs was particularly obvious in the thymus. the depletion of lymphoid cells from immunocompet ...	1988	3388762
[analysis of degradative plasmids by pyrolysis gas liquid chromatography].		1988	3388828
aeromonas eucrenophila species nova aeromonas caviae a later and illegitimate synonym of aeromonas punctata.	aeromonas caviae is a later and illegitimate synonym of aeromonas punctata. in order to secure continuity in literature, it is proposed to use the trivial term "caviae type" in connection with the species name a. punctata temporarily especially for strains isolated in connection to diarrhoea in children. aeromonas eucrenophila sp. nov. is described, type strain in ncmb 74. the new aerogenic psychrotrophic aeromonas species is separated genotypically from aeromonas hydrophila and aeromonas puncta ...	1988	3394446
oxolinic acid in the trout: bioavailability and tissue residues.	a study was performed on the serum bioavailability and tissue elimination of oxolinic acid in the trout. the compound was added to the diet and administered at a dosage-level of 12 mg/kg/day for 7 consecutive days. the study utilized an analytical technique, high performance liquid chromatography, which has been described in detail here. the results obtained demonstrate that serum concentrations higher than the mic for the control of the target pathogens (aeromonas and yersinia) can by sustained ...	1988	3400972
[frequency of aeromonas species in the stools of healthy and diarrheic patients].	during two test periods (summer 1985 and january-august 1986) a total of 7713 stool specimens were examined for the occurrence of aeromonas spp. the isolation rate of the asymptomatic group was about 0.3%, the same rate was found in adults with diarrhea. in the stool specimens of 364 children 8 aeromonas spp. could be found. a. caviae was isolated in most cases of diarrhea. data obtained suggest that it seems to be useful to screen all diarrhoic stools of children for the occurrence of aeromonas ...	1988	3402999
surface properties of autoagglutinating mesophilic aeromonads.	the surface characteristics of 24 autoagglutinating (aa+) mesophilic aeromonads were investigated. one group of 16 was found to be highly related serologically by their reactive pattern against o antisera generated against three reference strains. subsequent characterization of 11 of these isolates (group 1) indicated that they had the following properties in common: precipitation after boiling (pab+), membership of serogroup o:11 (typing scheme of sakazaki and shimada), resistance to lysis by b ...	1988	3417353
[antibiotic resistance of several gram-negative bacteria from clinical and dissection material from the institute for poultry diseases of ludwig maximilian university, munich].	a survey is given on antibiograms carried out with important bacterial strains isolated from birds during 1987. as compared with the results from earlier years no significant changes could be shown, the sensitivity ranging from 42.4% to 95.9%. the genera bordetella and aeromonas were included for the first time.	1988	3420603
osteomyelitis secondary to trauma or infected contiguous soft tissue.	nonhematogenous osteomyelitis (nho) occurred in 24 pediatric patients (ages 8 months to 18 years; median, 14 years; 23 male) admitted from 1980 to 1985. predisposing factors included compound fracture (12), deep decubiti (4) and foot puncture (3). infection involved tibia (7), foot bones (6), proximal femur (3) and ulna (2). patients presented with drainage (64%), pain or tenderness (44%) and fever (32%) lasting for 1 to 180 days (median, 10 days). in 24% both white blood cell count and erythroc ...	1988	2893333
postoperative skin-flap decongestion, leeches and aeromonas hydrophila.		1988	2895145
travelers' diarrhea in west africa and mexico: fecal transport systems and liquid bismuth subsalicylate for self-therapy.	the goals of this study were threefold: to compare the etiology of travelers' diarrhea in west africa and mexico, to evaluate two fecal transport systems for the recovery of enteropathogens, and to verify the efficacy of liquid bismuth subsalicylate (bss) in different locations and under different entrance criteria for disease severity. the study populations consisted of 133 european tourists in west africa and 112 american students in mexico who had suffered from travelers' diarrhea. in 60% and ...	1988	2896219
cefotaxime-sensitive aeromonas hydrophila infection in a revascularized foot.	three days following revascularization of a foot injured in a boating accident, aeromonas hydrophila cellulitis developed in the victim's foot and leg. the infection resolved with debridement and 10 days of cefotaxime therapy. a. hydrophila infection has not previously been reported in a revascularized extremity. clinical response of an a. hydrophila cellulitis to cefotaxime is likewise undescribed. our findings of a cefotaxime-sensitive aeromonas infection and its successful treatment suggests ...	1988	2896484
epidemiology of aeromonas species in a hospital.	the occurrence of aeromonas spp. in a hospital water supply, in patients and in the local community was investigated. in healthy persons outside the hospital the isolation rate was 3.6% and among hospital patients it was 6%. seven per cent of water samples yielded aeromonas strains. isolates were typed by sodium dodecyl sulphate polyacrylamide gel electrophoresis of sulphur-35 methionine labelled proteins of aeromonas isolates. no relationship between water and human isolates could be establishe ...	1988	2896686
adhesion of clinical and environmental aeromonas isolates to hep-2 cells.	a total of 63 aeromonas strains isolated from diarrhoeal faeces or water samples were tested for adhesion to hep-2 cells. an association between diarrhoea and high level adhesion was observed in that 12 of the 34 faecal isolates and none of the 29 environmental isolates yielded greater than 20 bacteria per hep-2 cell in the adhesion assay. the proportion of high adherers was significantly greater for a. sobria (57%) than for a. hydrophila isolates (19%). three of the eight faecal a. caviae isola ...	1988	2897470
[microflora of the drinking water regenerated from atmospheric moisture condensate in hermetically sealed quarters].	in an enclosed environment the species composition of microorganisms was quantified and metabolic processes in microbial cells isolated from the atmospheric condensate and reclaimed water were investigated. it was demonstrated that indicator microorganisms isolated from water and condensate samples (alcaligenes faecalis, citrobacter freundii, aeromonas hydrophila, acinetobacter-moraxella) were specific and differed from indicator microorganisms typical of regular tap water.	1988	2898035
[the occurrence of antibiotic-resistant aeromonas and vibrio species in river water].	262 samples of river water had been analysed for the occurrence of antibiotic resistant aeromonas and vibrio strains. the bacteria were cultured on selective media containing the antimicrobial chemotherapeutic agents oxytetracycline, chloramphenicol, streptomycin, ampicillin, kanamycin, gentamycin or trimethoprim. although it was not possible to identify antibiotic resistant bacteria of the genus vibrio, resistant strains of aeromonas hydrophila with a wide variety of resistance patterns could b ...	1988	2899367
houseplant peritonitis.		1988	2902398
isolation of theileria parva schizonts from infected lymphoblastoid cells.	this study set out to develop a rapid method for the isolation of schizonts from theileria parva-infected bovine and buffalo lymphoblastoid cells. parasitized lymphoblastoid cells were lysed by treatment with the cytolytic toxins, aerolysin and ah-1 hemolysin, produced by aeromonas hydrophila, and the schizonts were separated by percoll density-gradient centrifugation. light and electron microscopic examination showed that the isolated schizonts from lymphoblastoid cells infected with t. parva ( ...	1988	2903622
microbiological and virological analysis of water from two water filtration plants and their distribution systems.	the microbial flora of the water produced by two water filtration plants and their drinking water distribution system were evaluated: the pont-viau (pv) and the repentigny (re) water filtration plants. untreated water entering the plants contained 3.6 (pv) and 16.8 most probable number of infectious units (mpniu)/l (re) enteric viruses and total coliform bacteria counts were 300,000 (pv) and 500,000 cfu/l (re). treated water leaving the plant was essentially free of all the bacterial indicators ...	1988	2906813
correlation between toxin formation and diarrhoea in patients infected with aeromonas.		1988	2908356
comparison of four typing methods for aeromonas species.		1988	2908357
s-layer of high-virulence aeromonas hydrophila.		1988	2479668
serogrouping of the aeromonas hydrophila group.		1988	2479669
cloning and characterization of the aeromonas caviae reca gene and construction of an a. caviae reca mutant.	a recombinant plasmid carrying the reca gene of aeromonas caviae was isolated from an a. caviae genomic library by complementation of an escherichia coli reca mutant. the plasmid restored resistance to both uv irradiation and to the dna-damaging agent methyl methanesulfonate in the e. coli reca mutant strain. the cloned gene also restored recombination proficiency as measured by the formation of lac+ recombinants from duplicated mutant lacz genes and by the ability to propagate a strain of phage ...	1988	2826405
dna homology and immunological cross-reactivity between aeromonas hydrophila cytotonic toxin and cholera toxin.	dna colony hybridization with three 18- to 20-base-long synthetic oligonucleotide probes for cholera toxin (ct) was used to screen 12 clinical isolates of aeromonas hydrophila. under stringent hybridizing (overnight at 40 degrees c) and washing (1 h at 50 degrees c) conditions, nine strains reacted with the 32p-labeled ct probes. concentrated (10x) cell-free supernatants or lysates from eight cultures, heated at 56 degrees c for 20 min, produced cytotonic effects in y-1 mouse adrenal cells and c ...	1988	2830300
development and testing of invasion-associated dna probes for detection of shigella spp. and enteroinvasive escherichia coli.	genetic determinants of the invasive phenotype of shigella spp. and enteroinvasive escherichia coli (eiec), two common agents of bacillary dysentery, are encoded on large (180- to 210 kilobase), nonconjugative plasmids. several plasmid-encoded antigens have been implicated as important bacterial ligands that mediate the attachment and invasion of colonic epithelial cells by the bacteria. selected invasion plasmid antigen (ipa) genes have recently been cloned from shigella flexneri serotype 5 int ...	1988	2830310
tn5-induced protease-deficient strains of aeromonas hydrophila with reduced virulence for fish.	protease-deficient strains of aeromonas hydrophila tf7 were induced by transposon tn5 mutagenesis, with escherichia coli 1830(pjb4ji) as the tn5 donor. the parent strain has the cell surface characteristics associated with virulence for fish, and as it produces a single metalloprotease, mutants could be distinguished by direct plating on brain-heart infusion skim milk agar. mutants pd-7 and pd-10 still produced metalloprotease, but at reduced levels and only after prolonged incubation. the activ ...	1988	2843466
aerolysin from aeromonas hydrophila.		1988	2852762
in vitro activity of azithromycin (cp-62,993), a novel macrolide, against enteric pathogens.	azithromycin (cp-62,993 [9-deoxy-9a-methyl-9a-aza-9a-homoerythromycin]) is a novel macrolide antimicrobial. in this study the in vitro activity of cp-62,993 has been determined against selected enteropathogens, including clostridium difficile, and compared with that of erythromycin. mics were determined using an agar incorporation technique in mueller-hinton medium, containing saponin-lysed horse blood at a final concentration of 10% v/v, with an inoculum of 10(4) cfu. cp-62,993 was considerably ...	1988	2854515
virulence factors from aeromonas species.	isolates (116) of aeromonas were obtained from various sources and subjected to tests to establish their virulence factors. a high number of the isolates (69.8%) were found to be enterotoxigenic. the isolates from snails had more enterotoxigenic strains (73.3%), while those from cattle faeces had the lowest (33.3%). haemolysin production was found to be high (60.3%) amongst the isolates, and human isolates gave the highest number of haemolysin producing strains (70.6%), while the least number (3 ...	1988	2468071
molecular cloning, characterization, and nucleotide sequence of an extracellular amylase gene from aeromonas hydrophila.	the structural gene for excreted amylase from aeromonas hydrophila jmp636 has been cloned within a 2.1-kilobase smai fragment of dna. the amylase gene is transcribed from its own promoter in escherichia coli, producing a gene product of mr 49,000. the amylase gene product is secreted to the periplasm of e. coli; however, it is not excreted. nucleotide sequencing revealed an open reading frame of 1,392 base pairs corresponding to a protein of 464 amino acid residues. a potential signal peptide of ...	1988	2449422
comparison of four hemolysin-producing organisms (escherichia coli, serratia marcescens, aeromonas hydrophila, and listeria monocytogenes) for release of inflammatory mediators from various cells.	we investigated the role of various hemolysin-producing strains (escherichia coli, serratia marcescens, aeromonas hydrophila, and listeria monocytogenes) in induction of inflammatory mediators, e.g., histamine release from rat mast cells as well as the chemiluminescence response and the release of lipoxygenase transformation products from human polymorphonuclear neutrophils. our data show that the hemolysin-positive bacteria as well as the hemolysin-positive culture supernatants were active in i ...	1988	2451679
gastro-enteritis in australian children: studies on the aetiology of acute diarrhoea.	a prospective, year-long study of the microbiology of childhood gastro-enteritis in perth showed that (i) aeromonas-associated diarrhoea was the commonest recognized bacterial cause for acute diarrhoea, especially in summer, (ii) campylobacter infections were the next most common bacterial cause, (iii) rotavirus and parasites were not significant causes of sporadic acute childhood diarrhoea, and (iv) enteric bacterial pathogens and intestinal parasites were often isolated from aboriginal childre ...	1988	2456732
shigella flexneri invasion plasmid antigens b and c: epitope location and characterization with monoclonal antibodies.	invasion plasmid antigens b (ipab) and c (ipac) are associated with the ability of shigellae to invade cultured mammalian cells. monoclonal antibodies against ipab and ipac polypeptides were produced and used in a whole-cell enzyme-linked immunosorbent assay to show that both ipab and ipac polypeptides were exposed on the surface of virulent shigellae. moreover, these surface epitopes were shown to be highly conserved among different serotypes of shigella spp. and enteroinvasive escherichia coli ...	1988	2459066
nucleotide sequence and transcriptional analysis of the aercaera region of aeromonas sobria encoding aerolysin and its regulatory region.	the nucleotide sequence of a 2510 base pair chromosomal fragment containing the aerolysin gene aera, and its regulatory region aerc, from a clinical isolate of aeromonas sobria was determined. the aerolysin gene coded for a 54.5 kd polypeptide and had a g + c content of 59%, indicating that it is endogenous to the genus aeromonas. in contrast, the aerc region was characterized by its high a + t content (61%) and the presence of a core motif, aataaaa, repeated eight times within 300 base pairs. a ...	1988	2459581
in vitro activity of lomefloxacin (sc-47111; ny-198), a difluoroquinolone 3-carboxylic acid, compared with those of other quinolones.	lomefloxacin (sc-47111; ny-198) is a new difluoroquinolone agent. it inhibited 90% of escherichia coli, klebsiella spp., enterobacter spp., citrobacter spp., proteus mirabilis, morganella morganii, proteus vulgaris, serratia marcescens, salmonella spp., shigella spp., aeromonas spp., yersinia spp., haemophilus influenzae, and neisseria gonorrhoeae at less than or equal to 2 micrograms/ml. lomefloxacin inhibited 90% of pseudomonas aeruginosa at 4 micrograms/ml. lomefloxacin was equal in activity ...	1988	3164987
aeromonas hydrophilia infection in burn patients.	aeromonas hydrophilia rarely infects burn wounds. three cases of early a. hydrophilia burn wound infection, with one death, are reported. a history of extinguishing the fire with dirty water or by rolling in dirt should alert the physician to consider a. hydrophilia as a possible infection organism.	1988	3167587
suppurative thrombophlebitis due to aeromonas.	a patient developed lethal suppurative thrombophlebitis and adjacent soft-tissue infection caused by aeromonas. potential risk factors included corticosteroid therapy and the use of warm tap water compresses at the site of intravenous catheter-related phlebitis. this case demonstrates the rapidly invasive characteristics of aeromonas and the need for early surgical intervention in suppurative thrombophlebitis.	1988	3168427
dna:dna reassociation analysis of aeromonas salmonicida.	dna from 26 aeromonas salmonicida strains, namely 11 'typical' and 15 so-called 'atypical' strains, was used to assess the taxonomic relatedness within the species. the genomes were characterized by determination of dna base composition, dna:dna reassociation, calculation of sequence divergence following reassociation, and by genome size estimations. by comparison with dna obtained from controls and the aeromonas hydrophila group, a. salmonicida strains were determined to be correctly placed wit ...	1988	3171541
[the occurrence of aeromonas hydrophila in birds].		1988	3176762
isolation and characterization of motile aeromonas from human, food and environmental specimens.	from july 1985 to march 1987, the occurrence of motile aeromonas sp. in stool, food and environmental specimens was investigated to assess their pathogenic significance and to determine sources and routes of infection. a total of 9366 stool specimens were examined; aeromonas was isolated from 11.1% of diarrhoeal stools and 2.2% of normal stools (p less than 0.001). aeromonas counts in food specimens, which included minced beef, pork and chicken, seafood and various vegetables and their products, ...	1988	3181307
the in-vitro activity of pd127,391, a new quinolone.	mics of pd127,391 a new 4-quinolone, and of ci934 and ciprofloxacin, two previously reported 4-quinolones, were determined for common clinical bacterial isolates by an agar-dilution method. pd127,391 was the most active drug against enterobacteriaceae and acinetobacter spp (mics less than 0.12 mg/l) and as active as ciprofloxacin against aeromonas spp. (mics less than 0.008 mg/l) and pseudomonas aeruginosa (mics less than 1 mg/l). it was more active than ciprofloxacin against pseudomonas spp. in ...	1988	3182416
the comparative in-vitro activity of ofloxacin.	the antibacterial activity of ofloxacin, a new fluoroquinolone, was evaluated against a wide range of clinical bacterial isolates and compared with that of nalidixic acid, norfloxacin, enoxacin, pefloxacin and ciprofloxacin by determination of minimum inhibitory concentrations (mics). ofloxacin was very active against nalidixic acid-susceptible isolates of the enterobacteriaceae (mic less than or equal to 0.12 mg/l) and was also active against strains resistant to nalidixic acid (mic less than o ...	1988	3182468
comparison of blood agar, ampicillin blood agar, macconkey-ampicillin-tween agar, and modified cefsulodin-irgasan-novobiocin agar for isolation of aeromonas spp. from stool specimens.	the performance of four media for the isolation of aeromonas strains from stool specimens, the importance of ampicillin-susceptible aeromonas strains in the selection of culture media, and the usefulness of beta-hemolysis in screening blood-containing media for aeromonas strains were evaluated in two phases. in the first phase, 36 of 1,672 stool specimens yielded aeromonas isolates. ninety-seven percent of the isolates were detected on blood agar containing 20 micrograms of ampicillin per ml (ab ...	1988	3183020
[aeromonas hydrophila dacryocystitis. case report].	the present paper reports on a case of severe dacryocystitis with a dacryolith, caused by aeromonas hydrophila. on the basis of this case the authors show that a therapy effective in vitro does not lead to elimination of bacteria in all cases, so that further therapeutic considerations are necessary.	1988	3184754
aeromonas-associated diarrhea in children.	in a 27-month prospective study, aeromonas spp. were isolated from 7.3% of children with diarrhea and from 2.2% of controls. in 32 patients with diarrhea, ranging in age from 1 to 27 months old, aeromonas spp. were the only potential bacterial enteropathogens isolated. principal symptoms of aeromonas-associated diarrhea were vomiting, fever and bloody stools. diarrhea was often self-limiting and lasted for 10 days or less in 90% of patients. no secondary spread of diarrhea among close contacts w ...	1988	3186340
biochemical and toxigenic characteristics of aeromonas spp. isolated from diseased mammals, moribund and healthy fish.	in this study we describe biochemical, toxigenic and surface characteristics of 33 motile aeromonas isolated from diseased mammals, 3 from moribund marine mammals, 24 from healthy fish and 4 from moribund fish. aeromonas hydrophila, a. caviae and a. sobria were isolated from both mammals and fish but at a different incidence. aeromonas hydrophila was the predominant species isolated from clinical specimens; it was isolated from pneumonia, wound infections, septicemia and abortion in horses, catt ...	1988	3188376
the humoral immune response of rainbow trout (salmo gairdneri) immunised by various regimes and preparations of aeromonas salmonicida antigens.	antibody production in rainbow trout to extracellular antigens was investigated. the following antigen preparations and immunisation regimes were used: native extracellular products (ecp) in freund's complete adjuvant (fca), intraperitoneally (i.p.) with and without booster; formalinized ecp in fca, i.p. with and without booster; washed, formalinized a. salmonicida cells in fca, i.p., with booster; native ecp in saline, i.m., four weekly injections at two different doses, 45 micrograms and 6 mic ...	1988	3188377
in vitro activity of u-76,252 (cs-807), a new oral cephalosporin.	u-76,252 is the prodrug of u-76,253. mics of u-76,253 were 0.015 to 0.06 microgram/ml for greater than or equal to 90% of the strains of streptococcus spp., haemophilus influenzae, and proteus mirabilis; 0.25 to 1 microgram/ml for branhamella catarrhalis, escherichia coli, klebsiella spp., and citrobacter diversus; 1 to 8 micrograms/ml for staphylococcus spp.; and 2 to greater than 16 micrograms/ml for other members of the family enterobacteriaceae and aeromonas hydrophila; for 72% of the latter ...	1988	3190194
selection for virulence in the fish pathogen aeromonas salmonicida, using coomassie brilliant blue agar.	coomassie brilliant blue agar was used to quantify the frequency of the a-layer phenotype in different isolates of aeromonas salmonicida. hydrophilic, non-clumping isolates of a. salmonicida consisted predominantly of the a-layer minus phenotype. these bacteria were avirulent by intraperitoneal injection into susceptible brook trout (salvelinus fontinalis) and could not be reisolated from infected fish. by contrast, hydrophobic, clumping isolates were predominantly of the a-layer positive phenot ...	1988	3193563
inhibitory activity of antibiotic-producing marine bacteria against fish pathogens.	the activity of antibiotic-producing marine bacteria was assayed against bacterial fish pathogens belonging to the genera vibrio, aeromonas, pasteurella, edwardsiella, yersinia and pseudomonas with the aim of evaluating the possible use of these marine strains for controlling epizootics in aquaculture. inhibition tests on solid medium showed that, in general, the majority of fish bacteria were strongly sensitive to the marine bacteria. only two strains (edwardsiella tarda and pseudomonas aerugin ...	1988	3204073
the nucleotide sequence of the argt locus of aeromonas hydrophila.		1988	3205735
serological comparison between isolated strains of aeromonas salmonicida by agglutination and enzyme-linked immunosorbent assay (elisa).		1988	3206945
antibiotic sensitivities of aeromonas hydrophila cultured from medicinal leeches.	increased use of medicinal leeches (hirudo medicinalis) for the treatment of venous congestion in flaps and replanted parts has coincided with reports of soft tissue infections following leech application. we cultured the gullets of 20 medicinal leeches to re-examine the antibiotic sensitivities of aeromonas hydrophila, the leech enteric organism associated with reported infections. these isolates reflected reported resistance to penicillin and first generation cephalosporins as well as sensitiv ...	1988	3207967
[bacteria reducing chromium in nature and in effluents of industrial plants].	bacteria capable of hexavalent chromium (cr6+) reduction can be found in cr6+-containing sewage and sediments of purification tanks of industrial plants. they cannot be detected in water and soil samples containing no chromium compounds. bacteria reducing chromium belong to the genera aeromonas, escherichia, pseudomonas and enterobacter. their activity of cr6+ reduction correlates with the high resistance to the elevated content of this ion in the medium. the fine cell structure of these bacteri ...	1988	3211011
evaluation of several selective media for recovery of aeromonas hydrophila from polluted waters.	eleven media were studied for their suitability in the selective isolation of aeromonas hydrophila. preliminary results showed that five of them (inositol-brilliant green-bile salts agar, bile salts-brilliant green agar, rimler-shotts agar, xylose-sodium deoxycholate-citrate agar, and dextrin-fuchsin-sulfite agar) allowed the growth of several microorganisms that are usually present in the same samples in which a. hydrophila is found. six media (ma agar, modified rimler-shotts agar, dnase-toluid ...	1988	3214158
nutritional versatility and growth kinetics of an aeromonas hydrophila strain isolated from drinking water.	the nutritional versatility and growth kinetics of aeromonas hydrophila were studied to determine the nature and the growth-promoting properties of organic compounds which may serve as substrates for the growth of this organism in drinking water during treatment and distribution. as an initial screening, a total of 69 different organic compounds were tested at a concentration of 2.5 g/liter as growth substrates for 10 a. hydrophila strains. of these strains, strain m800 attained the highest maxi ...	1988	3214162
enteropathogenicity of aeromonas species isolated from infants: a cohort study.	the significance of aeromonas spp. as potential enteric pathogens was evaluated in a cohort of 187 infants aged 3-18 months during a 16-week summer period. aeromonas spp. were isolated from 14 of the 196 (7.1%) diarrhoeal episodes detected and from eight (5.2%) of 153 samples from paired asymptomatic infants. carriage of bacterial enteropathogens excluding aeromonas spp. was detected in a high proportion (23%) of the asymptomatic children. almost all of the seven isolates of aeromonas sobria wer ...	1988	3216131
[toxic properties of lipopolysaccharide and lipid a of aeromonas sobria and non 01 vibrio cholerae].		1988	3216765
[immunogenic and antigenic properties of bacterial cells, lipopolysaccharides and lipids a of aeromonas sobria and non 01 vibrio cholerae].		1988	3216766
[which are the most frequently occurring enteropathogenic bacteria in clinical practice?].	diarrhea is an increasingly frequent clinical finding in swiss medical practice. one reason is the volume of tourism in countries with different climatic and social circumstances. to obtain an overview of the enteropathogenic bacteria spectrum as a cause of diarrhea, we analyzed 11,163 stool specimens from a representative group of patients throughout switzerland. enteropathogenic bacteria were found in 15.2% of all specimens; 50% were campylobacter jejuni/coli, 35% salmonella spp., 8% aeromonas ...	1988	3217775
carbon substrate assimilation patterns of clinical and environmental strains of aeromonas hydrophila, aeromonas sobria and aeromonas caviae observed with a micromethod.	the assimilation of carbon substrates by 103 strains of aeromonas of different origin identified by conventional methods was studied by means of a standardized micromethod containing 147 tests (api system). six distinct groups could be recognized and the discriminating substrates were determined. 3 species of aeromonas can be identified by means of conventional method: a. hydrophila, a. sobria and a. caviae. the method has a number of drawbacks: some media are unreliable, others are difficult to ...	1988	3218392
[the occurrence of bacterial infections in the swamp beaver (myocaster coypus molina, 1782). 3. streptococcal, staphylococcal, aeromonas and actinobacillus infections].		1988	3219032