Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| [pre- and postoperative radiotherapy of laryngeal cancer]. | 2005 | 1196026 | |
| [letter: adverse effects of antiepileptic drugs]. | 2005 | 1183340 | |
| [extraction of amitriptyline with organic solvents]. | 2005 | 1175818 | |
| hearing loss of acquired syphilis: diagnosis confirmed by incudectomy. | syphilis, both congenital and acquired, may cause sensori-neural hearing loss. congenital syphilis presents in two forms: early (infantile), and late (tardive). acquired syphilis, both secondary and tertiary, may cause deafness. the clinical course of the acquired and congenital forms are similar. sensori-neural hearing loss with low discrimination scores may affect both ears simultaneously or sequentially, and vestibular complaints are common. a clinical diagnosis can be made on the basis of hi ... | 2005 | 1195972 |
| [obstructive cardiomyopathy. a new surgical method of treatment]. | a case of treatment of obstructive cardiomyopathy by insertion of a disc mistral prosthesis (cooley's technique) is reported. the result obtained was satisfactory from a clinical, haemodynamic and angiocardiographic standpoint. the mode of action of this technique is discussed. | 2005 | 1168899 |
| my years in local government. | 2005 | 1111872 | |
| lactase insufficiency in chinese children detected by oral milk and lactose challenge. | a study to determine the incidence of late-onset lactose intolerance was undertaken in 68 normal chinese children in singapore using measurements of breath hydrogen concentrations, following a lactose or milk challenge, as indicator of lactose malabsorption. evidence of lactose malabsorption was detected in 26% of children given a lactose challenge, and in 21% of those given a milk challenge. it is concluded that late-onset lactase insufficiency is common in chinese children in singapore. | 2005 | 2441643 |
| [immunoblastic lymphadenopathy and toxacariasis. report of a case]. | this is a report of a case a larval granulomatosis associated with immunoblastic lymphadenopathy, corroborated by laboratory and histologic studies compatible with that. the etiopathogenesis, clinical picture and diagnosis of both diseases are commented, and the possible relationship between them is stressed. | 2005 | 1087413 |
| [diagnosis of kartagener's syndrome]. | 2005 | 1081308 | |
| dna synthesis and turnover in the bullfrog tadpole during metamorphosis. | 125i-labeled deoxyuridine (idurd) has been used to estimate the turnover of dna in liver, tail, and hind limb during spontaneous and triiodothyronine-induced metamorphosis. it was found that the total amount of liver dna remained constant and there was no significant loss of the label from the liver dna, which would be expected if there was an increase in dna turnover during metamorphosis. also, the change in specific activity of liver dna parallels that of tail dna during spontaneous metamorpho ... | 2005 | 1084345 |
| [lupins - a new source of food for andean countries. 5. traditional methods of debittering of lupins by water]. | the bitter taste of lupin alkaloids can be sensorially detected in water within ppm-range. the strength of the taste diminishes as follows: sparteine, d-lupanin-perchlorate, lupinine, isolupinine and hydroxylupanine. the swelling capacity of lupin seeds presents different characteristics according to the species. the swelling rapidity is in l. albus somehow inferior to that of l. mutabilis. the absorption of water decreases in hot water, and the swelling rapidity increases. for domestic debitter ... | 2005 | 556411 |
| etiopathogenesis of degenerative joint diseases. | 2005 | 1064949 | |
| dental treatment in the hemophiliac. | 2005 | 1064933 | |
| [naevobasaliomatosis (gorlin-goltz syndrome)]. | 2005 | 551607 | |
| comparison of analytical methods for residual ethylene oxide analysis. | a round-robin study compared four methods of residual ethylene oxide analysis. results from the six participating laboratories, along with a statistical treatment of the data, are presented. | 2005 | 1151720 |
| decision-making in medical research and clinical practice: theory and methodology of laboratory data evaluation by predictors, indicators and indices. | 2005 | 1196136 | |
| the differentiation of cytotoxic t lymphocytes in vitro: an ultrastructural study. | t killer cells were identified morphologically in two different culture systems. the first system allowed identification by comparing under identical culture conditions two types of stimulation, one leading to cytotoxic activity levels that were very high (h-2 stimulation) and the other to levels which were practically nil (m-locus stimulation). a second system allowed identification by the use of a collaborative system in which relatively pure precursors of t killer cells were mixed with precur ... | 2005 | 1082798 |
| [aspects of culture-media research within the scope of industrial vaccine production. summary of twenty-year development work in the institute for vaccines dessau]. | 2005 | 4725026 | |
| stroke rehabilitation. part i. general considerations. | 2005 | 1214691 | |
| [the candida prostato-urethritis]. | 2005 | 1196083 | |
| interleukin-1 inhibits pge2 binding to macrophage-like p388d1 cells by a cyclic amp-independent process. | the interaction between interleukin il-1 alpha and pge2 on p388d1 cells has been investigated. preincubation of murine macrophage-like cells, p388d1, with il-1 alpha (0-73 pm) reduced the binding of pge2 to these cells in a concentration-dependent manner. scatchard analysis showed that il-1 alpha decreased the pge2 binding by lowering both the high and low affinity receptor binding capacities (from 0.31 +/- 0.02 to 0.12 +/- 0.01 fmol/10(6) cells for the high affinity receptor binding sites and f ... | 2005 | 1310627 |
| diphtheria toxin entry: protein translocation in the reverse direction. | 2005 | 3072713 | |
| intracellular recordings of rod responses during dark-adaptation. | 1. dark-adaptation of rod photoreceptors has been studied in the isolated axolotl (ambystoma mexicanum) retina by intracellular recordings. rod responsiveness was greatly reduced immediately after a 30 sec partial bleach, but partially recovered with time in the dark. 2. in parallel spectrophotometric measurements using isolated retinas, regeneration of the rod pigment could not be detected after a 30 sec bleach. 3. during rod dark-adaptation, the response of a rod to a given stimulus increased ... | 2005 | 1151778 |
| transient response of a solid-liquid model biological fluidised bed to a step change in fluid superficial velocity. | the evolution of a solid-liquid model biological fluidised bed under a step change in fluid superficial velocity is described. during a transient step change, the fluidised bed divides into a top zone which remains at the initial porosity and a bottom zone which settles at the final porosity. the interface of discontinuity in porosity moves progressively upwards through the fluidised bed. the velocity at which the top of the fluidised bed expands or contracts and the upward velocity of the poros ... | 2005 | 1366699 |
| [spontaneous closure of the interventricular septal defects in children]. | a total of 214 patients with a defect of the interventricular septum have been examined starting from the first days of their life and to the age of 7 years. in 50 of them the defect closed spontaneously (23.4 per cent). twenty of these patients had a complicated course of the defect (circulatory insufficiency, colds and pneumonia). the spontaneous closure of the interventricular septum defect occurred most frequently at the age of from 1 to 4 years (86 per cent). | 2005 | 1142609 |
| [emergency treatment of acute promyelocytic leukemia and its hemorrhagic complications. apropos of 30 cases]. | 2005 | 1065249 | |
| lymphocyte markers and circulating immune complexes in primary glomerulonephritis. | 2005 | 549018 | |
| [outpatient care in industrial communities as a means of reducing periodontal diseases]. | 2005 | 545607 | |
| the chemical use, abuse, and dependence scale (cuad). rationale, reliability, and validity. | this article describes the rationale for the development of the chemical use, abuse, and dependence scale (cuad). the instrument is in a semistructured interview format; it derives both substance use severity scores and dsm-iii-r substance use disorder diagnoses and can be administered in a short period with minimal training. the reliability and validity of the cuad are reported and appear satisfactory. the cuad is recommended for use as a detection, diagnostic, and treatment selection index in ... | 2005 | 1317464 |
| myelin specific, autoaggressive t cell clones in the normal immune repertoire: their nature and their regulation. | 2005 | 1285063 | |
| part ii: the photic environment of clear tropical seas during the day. | 2005 | 1166605 | |
| projections from the retina to the lateral geniculate nucleus and mesencephalic tectum in a reptile (tupinambis nigropunctatus): a comparison of anterograde transport and anterograde degeneration. | 2005 | 1111840 | |
| [massive lower gastrointestinal hemorrhage of diverticular origin. diagnostic and therapeutic problems]. | 2005 | 1082809 | |
| inflammatory response linked to oxazolone-indued cutaneous basophil hypersensitivity: effect of different immunomodulator and anti-inflammatory drugs. | the potency of cytostatic and anti-inflammatory drugs was tested on the oxazolone-induced cutaneous basophil hypersensitivity (cbh) in mice. the challenge reaction was performed early after sensitization in order to minimize b-cell expression; exudative and cellular infiltration was estimated 6 h, 24 h and 48 h after challenge. the potency of drugs was tested at three different periods of immunization: 2 days before or after sensitization or before challenge. cytostatics act mainly when cells ar ... | 2005 | 546176 |
| [rupture of the distal tendon of the biceps brachii]. | 2005 | 538842 | |
| [skin diseases caused by cocci]. | 2005 | 1214698 | |
| five years' experience with aortocoronary bypass grafting. | during a 5-year period (apr. 14, 1970 to apr. 14, 1975) 930 patients underwent aortocoronary bypass grafting; the procedure was done as an emergency in 141. of the entire group 3.3% died at operation, 1.6% died in hospital and 5.8% died later; of the patients undergoing emergency grafting 12.1% died at operation and 5.7% died later. from a detailed analysis of the first 600 patients it was found that both operative and late mortality were clearly related to two factors: severe left ventricular d ... | 2005 | 1082791 |
| acute toxicity of mevinphos to gerbils. | 2005 | 1273839 | |
| [effect of chronic irradiation in low doses on age-related changes in the radiation-resistance of white mice]. | 2005 | 1188055 | |
| a syndrome of acute zinc loss. cerebellar dysfunction, mental changes, anorexia, and taste and smell dysfunction. | oral administration of the amino acid histidine to six patients with progressive systemic sclerosis produced anorexia, taste ans smell dysfunction, changes in mentation, and cerebellar dysfunction in each patient; these changes were associated with significant decreases in serum zinc concentration and significant increases in urinary zinc excretion. administration of zinc ion, even with continued histidine administration, returned each of the signs and symptoms to or toward normal within 8 to 24 ... | 2005 | 1180744 |
| the aching mouth and the aching psyche. | 2005 | 1074368 | |
| [lipid and mineral metabolism and morbidity with temporary loss of work capacity in miners]. | examination is performed on 87 miners from mine for copper production subject to intensive local vibrations with average rate of increase (k) of the norms for the individual octave frequencies 2.4 times for 3 h exposure of the working shift, as well as to intensive noise--113-115 db/a equivalent level, general dust--23 mg/m3, fine dust--3.5 mg/m3 and fine quartz--0.43 mg/m3. there are data for vibration effect--changes in the cold test, vibration sensoriness, ultra sound sonometry, in all miners ... | 2005 | 1364539 |
| preparation, crystallization and preliminary x-ray crystallographic studies of diadenosine tetraphosphate hydrolase from shigella flexneri 2a. | diadenosine tetraphosphate (ap4a) hydrolase (ec 3.6.1.41) hydrolyzes ap4a symmetrically in prokaryotes. it plays a potential role in organisms by regulating the concentration of ap4a in vivo. to date, no three-dimensional structures of proteins with significant sequence homology to this protein have been determined. the 31.3 kda ap4a hydrolase from shigella flexneri 2a has been cloned, expressed and purified using an escherichia coli expression system. crystals of ap4a hydrolase have been obtain ... | 2005 | 16511239 |
| immunoproteomics of membrane proteins of shigella flexneri 2a 2457t. | to screen the immunogenic membrane proteins of shigella flexneri 2a 2457t. | 2005 | 16425402 |
| [the colicynogenic activity and anthibiotic sensitivity of collected and newly isolated strains of shigella flexneri and shigella sonnei]. | among the collection strains of s. flexneri 10.5; 5.5; 2.6; 2.6 and 7.9% of strains were resistant to ampicillin, tetracycline, ceftryaxon, cefasoline, chloramphenycol, respectively. the maximum number of r-determinants of one strain was 4. the strains resistant to cefalosporynes were not revealed; among newly isolated strains of this agent, these strains resistant to ampicilline, tetracycline and chloramphenycol made 20%. among the collection and newly isolated strains of s. flexneri, isolated ... | 2005 | 16396114 |
| detection and isolation method for bacterial lysozyme inhibitors by use of lysozyme affinity chromatography. | 2005 | 16366278 | |
| a ph dependent mechanism for inactivation of gram-negative bacteria by hen egg white and lambda lysozymes under high pressure. | 2005 | 16366309 | |
| identification of core and variable components of the salmonella enterica subspecies i genome by microarray. | we have performed microarray hybridization studies on 40 clinical isolates from 12 common serovars within salmonella enterica subspecies i to identify the conserved chromosomal gene pool. we were able to separate the core invariant portion of the genome by a novel mathematical approach using a decision tree based on genes ranked by increasing variance. all genes within the core component were confirmed using available sequence and microarray information for s. enterica subspecies i strains. the ... | 2005 | 16299280 |
| flashlights on bacterial virulence proteins. | 2005 | 16299473 | |
| secretion of type iii effectors into host cells in real time. | type iii secretion (t3s) systems are key features of many gram-negative bacteria that translocate t3s effector proteins directly into eukaryotic cells. there, t3s effectors exert many effects, such as cellular invasion or modulation of host immune responses. studying spatiotemporal orchestrated secretion of various effectors has been difficult without disrupting their functions. here we developed a new approach using shigella flexneri t3s as a model to investigate bacterial translocation of indi ... | 2005 | 16299482 |
| ready-to-eat shrimp as an international vehicle of antibiotic-resistant bacteria. | the occurrence of antibiotic-resistant bacteria in foods of animal origin is a potential health threat because resistance can be transferred among bacteria, and antibiotic-resistant pathogens may not respond to antibiotic treatments. thirteen brands of ready-to-eat shrimp representing four countries of origin were obtained from local grocery stores. total heterotrophic plate counts were determined, and antibiotic-resistant bacteria were isolated. total heterotrophic colony counts ranged from 3.3 ... | 2005 | 16300079 |
| purification and preliminary crystallographic studies of cutc, a novel copper homeostasis protein from shigella flexneri. | cutc is a novel copper homeostasis protein containing 248 amino acids. here we report the cloning, expression, purification, crystallization and preliminary x-ray crystallographic studies of cutc from shigella flexneri 2a. purification of cutc and its selenomethionine (semet) derivate were done using immobilized metal ion affinity chromatography, size-exclusion and ion-exchange chromatography. the purified proteins were crystallized using the hanging drop vapor diffusion method. the diffraction ... | 2005 | 16305556 |
| analysis of the mechanisms of resistance to several antimicrobial agents in shigella spp. causing travellers' diarrhoea. | eighty isolates of shigella spp. (37 shigella flexneri and 43 shigella sonnei) from patients with travellers' diarrhoea were studied. susceptibility tests revealed high levels of resistance, especially to ampicillin (65%), tetracycline (78%) and trimethoprim (75%), and particularly among the s. flexneri isolates. dihydrofolate reductase 1 genes (dfra1) were prevalent among the trimethoprim-resistant isolates, while oxa genes predominated among the ampicillin-resistant isolates. chloramphenicol r ... | 2005 | 16307563 |
| [significance of determination of shigella antibiotic resistance in bacteriological diagnosis of dysentery]. | the results of the shigella antibiotic susceptibility assay within 1995-2002 are presented. 1472 cultures from 1158 patients with intestinal infections and bacteria carriers were isolated. the isolates were tested for their susceptibility to tetracycline, chloramphenicol, gentamicin, kanamycin, ampicillin and ofloxacin. it was shown that s. flexneri and s. sonnei were resistant to tetracycline. the s. flexneri isolates were highly resistant to chloramphenicol (73.3 to 96.0%) while resistance to ... | 2005 | 16308937 |
| fur regulates acid resistance in shigella flexneri via ryhb and ydep. | shigella flexneri requires iron for survival, and the genes for iron uptake and homeostasis are regulated by the fur protein. microarrays were used to identify genes regulated by fur and to study the physiological effects of iron availability in s. flexneri. these assays showed that the expression of genes involved in iron acquisition and acid response was induced by low-iron availability and by inactivation of fur. a fur null mutant was acid sensitive in media at ph 2.5, and acid sensitivity wa ... | 2005 | 16313621 |
| [construction and characterization of a live attenuated shigella flexneri 2a vaccine strain, sf301 delta virg and dsba33g]. | construction and characterization of a live attenuated shigella flexneria 2a sf301 vaccine strain to prevent the endemic of shigellosis. using chinese majority epidemic shigella flexneri 2a serotype sf301 as the target, p delta virg, a deletion derivation of the virg gene in the sacb suicide vector pcvd442 and pdsba33g, an mutant of a disulfide bond catalyst dsba, replaced its 33 amiano acid cystine by glycerin in pcvd442, were used to generate a attenuated mutant strain sf301: delta virg: dsba3 ... | 2005 | 16342769 |
| quinine sulfate inhibits invasion of salmonella typhimurium and shigella flexneri: a preliminary study. | the incidence of typhoid fever and shigellosis parallels that of malaria, so many individuals who are on antimalarial drugs can be found in areas where these diseases are widespread. we investigated the effect of quinine sulfate on the growth and invasion of salmonella typhimurium and shigella flexneri m90t to determine whether people on antimalarials can have secondary gain from some protection against typhoid fever and shigellosis. | 2005 | 16343387 |
| risk factors for mortality due to shigellosis: a case-control study among severely-malnourished children in bangladesh. | to determine the risk factors for death of severely-malnourished bangladeshi children with shigellosis, a case-control study was conducted at the clinical research and service centre of icddr,b: centre for health and population research in dhaka, bangladesh. one hundred severely-malnourished children (weight-for-age <60% of median of the national center for health statistics), with a positive stool culture for shigella dysenteriae type 1 or s. flexneri, who died during hospitalization, were comp ... | 2005 | 16262023 |
| the mreb and min cytoskeletal-like systems play independent roles in prokaryotic polar differentiation. | establishment of an axis of cell polarity and differentiation of the cell poles are fundamental aspects of cellular development in many organisms. we compared the effects of two bacterial cytoskeletal-like systems, the mreb and mincde systems, on these processes in escherichia coli. we report that the min proteins are capable of establishing an axis of oscillation that is the initial step in establishment of polarity in spherical cells, in a process that is independent of the mreb cytoskeleton. ... | 2005 | 16262780 |
| construction of prophylactic human papillomavirus type 16 l1 capsid protein vaccine delivered by live attenuated shigella flexneri strain sh42. | to express human papillomavirus (hpv) l1 capsid protein in the recombinant strain of shigella and study the potential of a live attenuated shigella-based hpv prophylactic vaccine in preventing hpv infection, the icsa/virg fragment of shigella-based prokaryotic expression plasmid phs3199 was constructed. hpv type 16 l1 (hpv16l1) gene was inserted into plasmid phs3199 to form the phs3199-hpv16l1 construct, and phs3199-hpv16l1 was electroporated into a live attenuated shigella strain sh42. western ... | 2005 | 16270153 |
| shigella flexneri perinephric abscess and bacteremia. | 2005 | 16270768 | |
| genome dynamics and diversity of shigella species, the etiologic agents of bacillary dysentery. | the shigella bacteria cause bacillary dysentery, which remains a significant threat to public health. the genus status and species classification appear no longer valid, as compelling evidence indicates that shigella, as well as enteroinvasive escherichia coli, are derived from multiple origins of e.coli and form a single pathovar. nevertheless, shigella dysenteriae serotype 1 causes deadly epidemics but shigella boydii is restricted to the indian subcontinent, while shigella flexneri and shigel ... | 2005 | 16275786 |
| the burden of diarrhoea, shigellosis, and cholera in north jakarta, indonesia: findings from 24 months surveillance. | in preparation of vaccines trials to estimate protection against shigellosis and cholera we conducted a two-year community-based surveillance study in an impoverished area of north jakarta which provided updated information on the disease burden in the area. | 2005 | 16242013 |
| functional effects of japanese style fermented soy sauce (shoyu) and its components. | the functional effects of japanese style fermented soy sauce (shoyu) have been studied. soy sauce promotes digestion, because the consumption of a cup of clear soup containing soy sauce enhances gastric juice secretion in humans. soy sauce possesses antimicrobial activity against bacteria such as staphylococcus aureus, shigella flexneri, vibrio cholera, salmonella enteritidis, nonpathogenic escherichia coli and pathogenic e. coli o157:h7. soy sauce also contains an antihypertensive component. an ... | 2005 | 16243270 |
| helical packing of needles from functionally altered shigella type iii secretion systems. | gram-negative bacteria commonly interact with eukaryotic host cells using type iii secretion systems (ttsss or secretons), which comprise cytoplasmic, transmembrane and extracellular domains. the extracellular domain is a hollow needle-like structure protruding 60 nm beyond the bacterial surface. the ttss is activated to transfer bacterial proteins directly into a host cell only upon physical contact with the target cell. we showed previously that the monomer of the shigella flexneri needle, mxi ... | 2005 | 16243352 |
| frequency of isolation of shigella serogroups/serotypes and their antimicrobial susceptibility pattern in children from slum areas in karachi. | to assess the frequency of serogroups and serotypes, as well as the antimicrobial susceptibility pattern of shigella species isolated from known cases of diarrhoea and dysentery from karachi, pakistan. | 2005 | 15960281 |
| plastins: versatile modulators of actin organization in (patho)physiological cellular processes. | many actin-binding proteins are expressed in eukaryotic cells. these polypeptides assist in stabilizing and rearranging the organization of the actin cytoskeleton in response to external stimuli, or during cell migration and adhesion. here we review a particular set of actin-binding proteins called plastins. plastins (also called fimbrins) belong to a subclass of actin-binding proteins known as actin bundling proteins. three isoforms have been characterized in mammals: t-plastin is expressed in ... | 2005 | 15960882 |
| the shigella flexneri serotype y vaccine candidate sfl124 originated from a serotype 2a background. | shigella flexneri is endemic in most developing countries and responsible for the highest mortality rate among the shigella species. the attenuated serotype y s. flexneri strain sfl124 has been used as the parental strain for the development of recombinant vaccines expressing multiple o-antigen structures. during the development of one such multivalent vaccine, a region of gtrii homology was found in sfl124. sequencing and analysis of this region revealed the presence of an insertion element int ... | 2005 | 15963704 |
| diarrheal illness among deployed u.s. military personnel during operation bright star 2001--egypt. | in the fall of 2001, approximately 15,000 u.s. military personnel participated in a military exercise in the northwestern egyptian desert. to assess the prevalence and impact of diarrhea and enteropathogen distribution, we conducted a post-deployment survey and a case series study. a departure convenience sampling (n = 3725) was used in the post-deployment survey. overall, 9.3% reported diarrhea, 2.6% sought medical care, and 2.8% stopped or decreased their work for at least a day. among those r ... | 2005 | 15964494 |
| [effects of different compositions of gegenqinlian decoction on experimental shigellosis in rabbits]. | to investigate the relations between different compositions of gegenqinlian decoction and their effects on experimental shigellosis in rabbits to explore the composition protocol of gegenqinlian decoction. | 2005 | 16174580 |
| an unusual case of diarrhea. | the clue to this patient's correct diagnosis was his recent return from india. he usually visits there on an annual basis and the occurrence of posttravel infection has been noted in his medical records. this case study shows the importance of assessing patients for fever, abdominal cramps and diarrhea after recent travel outside of the united states. shigella gastroenteritis should be in the differential diagnosis for patients with the above clinical presentation. the keys for diagnosis and pro ... | 2005 | 16180784 |
| a bacterial group ii intron-encoded reverse transcriptase localizes to cellular poles. | the lactococcus lactis ll.ltrb group ii intron encodes a reverse transcriptase (ltra protein) that binds the intron rna to promote rna splicing and intron mobility. here, we used ltra-gfp fusions and immunofluorescence microscopy to show that ltra localizes to cellular poles in escherichia coli and lactococcus lactis. this polar localization occurs with or without coexpression of ll.ltrb intron rna, is observed over a wide range of cellular growth rates and expression levels, and is independent ... | 2005 | 16186487 |
| [dysentery in poland in 2003]. | the notified number of dysentery cases is the lowest in this century and even every year decreasing. only 75 cases were notified in the year 2003 (incidence rate 0.20/100 000 population) while 220 cases were registered in 2002 (incidence 0.58/100 000 population). no one death case was notified. the last three death cases were notified in 1999. only one outbreak of dysentery (due to s. flexneri 2a) was registered involving 23 patients/111 residents of social home for mentally disabled men. source ... | 2005 | 16190529 |
| abl kinases regulate actin comet tail elongation via an n-wasp-dependent pathway. | microbial pathogens have evolved diverse strategies to modulate the host cell cytoskeleton to achieve a productive infection and have proven instrumental for unraveling the molecular machinery that regulates actin polymerization. here we uncover a mechanism for shigella flexneri-induced actin comet tail elongation that links abl family kinases to n-wasp-dependent actin polymerization. we show that the abl kinases are required for shigella actin comet tail formation, maximal intracellular motilit ... | 2005 | 16199863 |
| the needle component of the type iii secreton of shigella regulates the activity of the secretion apparatus. | gram-negative bacteria commonly interact with eukaryotic host cells by using type iii secretion systems (ttsss or secretons). ttsss serve to transfer bacterial proteins into host cells. two translocators, ipab and ipac, are first inserted with the aid of ipad by shigella into the host cell membrane. then at least two supplementary effectors of cell invasion, ipaa and ipgd, are transferred into the host cytoplasm. how ttsss are induced to secrete is unknown, but their activation appears to requir ... | 2005 | 16227202 |
| the epidemiology of travel-associated shigellosis--regional risks, seasonality and serogroups. | to give a detailed risk estimate of contracting travel-associated shigellosis in various regions of the world. | 2005 | 16230220 |
| pathogenicity of shigella in healthy carriers: a study in vientiane, lao people's democratic republic. | shigella spp. isolated from diarrheal patients and non-diarrheal carriers were examined by pcr for the presence of two pathogenic genes, chromosomal ipah and invasive plasmid encoded ial. shigella spp. were detected in 7 of 72 diarrheal cases examined (9.7%), and 9 of 145 non-diarrheal cases (6.2%). all isolates from diarrheal cases harbored both ipah and ial, while all isolates from non-diarrheal cases were positive for ipah but not ial. these results suggested that shigella spp. in healthy car ... | 2005 | 16116257 |
| shigella flexneri serotype 3 infections among men who have sex with men--chicago, illinois, 2003-2004. | during 2003-2004, the chicago department of public health (cdph) investigated an increase in reported shigella flexneri serotype 3 infections among adult males. this report summarizes the investigation into those cases and underscores the potential for sexual transmission of enteric infections among men who have sex with men (msm). | 2005 | 16121121 |
| characterization of oak and birch dust-induced expression of cytokines and chemokines in mouse macrophage raw 264.7 cells. | occupational exposure to wood dust is related to several respiratory diseases, such as allergic rhinitis, chronic bronchitis, and asthma. however, virtually nothing is known about molecular mechanisms behind wood dust-induced pulmonary inflammation. to elucidate the effects of wood dust exposure on cytokine and chemokine expression in murine macrophage cell line cells, mouse raw 264.7 cells were exposed to two selected hardwood dusts, oak and birch. tio2 and lps were used as controls. expression ... | 2005 | 16122864 |
| synthesis and antimicrobial activity of some novel derivatives of benzofuran: part 1. synthesis and antimicrobial activity of (benzofuran-2-yl)(3-phenyl-3-methylcyclobutyl) ketoxime derivatives. | the reaction of salicylaldehyde with 1-phenyl-1-methyl-3-(2-chloro-1-oxoethyl) cyclobutane (1) and potassium carbonate was used to prepare (benzofuran-2-yl)(3-methyl-3-phenylcyclobutyl) methanone (2) for the starting reagent purposes. (benzofuran-2-yl)(3-phenyl-3-methyl cyclobutyl) ketoxime (3) was synthesized from the reaction of the compound (2) with hidroxylamine. new derivatives of (benzofuran-2-yl)(3-phenyl-3-methyl cyclobutyl) ketoxime (3) such as, o-glycidylketoxime (4) and o-phenylacylke ... | 2005 | 16129517 |
| optimal word sizes for dissimilarity measures and estimation of the degree of dissimilarity between dna sequences. | several measures of dna sequence dissimilarity have been developed. the purpose of this paper is 3-fold. firstly, we compare the performance of several word-based or alignment-based methods. secondly, we give a general guideline for choosing the window size and determining the optimal word sizes for several word-based measures at different window sizes. thirdly, we use a large-scale simulation method to simulate data from the distribution of sk-ld (symmetric kullback-leibler discrepancy). these ... | 2005 | 16144805 |
| [epidemiological situation in enteric infections in the republic of armenia]. | 2005 | 16146241 | |
| rapid differentiation among bacteria that cause gastroenteritis by use of low-resolution raman spectroscopy and pls discriminant analysis. | use of classical microbiological methods to differentiate bacteria that cause gastroenteritis is cumbersome but usually very efficient. the high cost of reagents and the time required for such identifications, approximately four days, could have serious consequences, however, mainly when the patients are children, the elderly, or adults with low resistance. the search for new methods enabling rapid and reagentless differentiation of these microorganisms is, therefore, extremely relevant. in this ... | 2005 | 16158301 |
| the shigella flexneri effector ospg interferes with innate immune responses by targeting ubiquitin-conjugating enzymes. | bacteria of shigella spp. are responsible for shigellosis in humans. they use a type iii secretion system to inject effector proteins into host cells and induce their entry into epithelial cells or trigger apoptosis in macrophages. we present evidence that the effector ospg is a protein kinase that binds various ubiquitinylated ubiquitin-conjugating enzymes, including ubch5, which belongs to the stem cell factor scf(beta-trcp) complex promoting ubiquitination of phosphorylated inhibitor of nf-ka ... | 2005 | 16162672 |
| genetic diversity of shigella species from different intercontinental sources. | one hundred and twenty three strains of shigella spp. (mostly shigella sonnei and shigella flexneri) were isolated between 1995 and 2000 from patients suffering from traveller's diarrhoea. seventy nine of them have been typed by digestion of their chromosomal dna with xba i and pulsed field gel electrophoresis. results show a high degree of heterogeneity in both s. sonnei and s. flexneri isolates. this is the first time that the molecular typing of such a high number of geographically unrelated ... | 2005 | 16168941 |
| the generalized transducing salmonella bacteriophage es18: complete genome sequence and dna packaging strategy. | the generalized transducing double-stranded dna bacteriophage es18 has an icosahedral head and a long noncontractile tail, and it infects both rough and smooth salmonella enterica strains. we report here the complete 46,900-bp genome nucleotide sequence and provide an analysis of the sequence. its 79 genes and their organization clearly show that es18 is a member of the lambda-like (lambdoid) phage group; however, it contains a novel set of genes that program assembly of the virion head. most of ... | 2005 | 15659686 |
| a novel intranasal protollin-based measles vaccine induces mucosal and systemic neutralizing antibody responses and cell-mediated immunity in mice. | protollin-mv is a vaccine produced by mixing split measles virus (mv) antigen with the novel adjuvant protollin (neisseria meningitidis outer membrane proteins non-covalently complexed with shigella flexneri 2a lipopolysaccharide). intranasal immunization of mice with two or three doses of protollin-mv induces both serum igg and mucosal iga with strong neutralizing activity. there is a dose-dependent shift towards lower igg1:igg2a ratios and mv-specific ifngamma production in splenocytes. intran ... | 2005 | 15661386 |
| modulation of an outer membrane protease contributes to the virulence defect of shigella flexneri strains carrying a mutation in the virk locus. | the shigella actin assembly protein icsa is removed from the bacterial surface by the protease icsp. we show that decreased intracellular spreading of virk::tn10 mutants is due in part to significant increases in icsp and icsp-mediated cleavage of icsa and that icsp expression is a critical determinant of shigella virulence. | 2005 | 15664968 |
| sensitive and rapid detection of shigella and enteroinvasive escherichia coli by a loop-mediated isothermal amplification method. | here we report a loop-mediated isothermal amplification (lamp) method for detecting shigella and enteroinvasive escherichia coli. the target for this lamp method is the ipah gene which is carried by both of the pathogens. the lamp method efficiently detected the gene within 2 h at a minimal amount of bacteria (8 cfu) per reaction. | 2005 | 15668027 |
| synthesis of two linear padre conjugates bearing a deca- or pentadecasaccharide b epitope as potential synthetic vaccines against shigella flexneri serotype 2a infection. | the blockwise synthesis of the 2-aminoethyl glycosides of a deca- and a pentadecasaccharide made of two and three repeating units, respectively, of the shigella flexneri serotype 2a specific polysaccharide is reported. the strategy relies on trifluoromethanesulfonic acid mediated glycosylation of a pentasaccharide building block acting as a glycosyl donor and a potential glycoside acceptor. both targets were made available in amounts large enough for their subsequent conversion into glycoconjuga ... | 2005 | 15669066 |
| physiological stress of intracellular shigella flexneri visualized with a metabolic sensor fused to a surface-reporter system. | when deleted of its n-terminal signal-reception domain, the broad host range sigma54-dependent transcriptional regulator xylr, along with its cognate promoter pu, becomes a sensor of the metabolic stress of the carrier bacteria. we have employed a surface reporter system to visualize the physiological status of intracellular shigella flexneri during infection of henle 407 cells in culture. to this end, the xylrdeltaa gene has been engineered adjacent to a bicistronic transcriptional fusion of pu ... | 2005 | 15670852 |
| cloning of a novel gene for quinolone resistance from a transferable plasmid in shigella flexneri 2b. | a novel gene for quinolone resistance was cloned from a transferable plasmid carried by a clinical isolate of shigella flexneri 2b that was resistant to fluoroquinolones. the plasmid conferred low-level resistance to quinolones on escherichia coli hb101. the protein encoded by the gene showed 59% amino acid identity with qnr. | 2005 | 15673773 |
| occurrence of integrons and resistance genes among sulphonamide-resistant shigella spp. from brazil. | to determine the occurrence of class 1 and 2 integrons and antimicrobial resistance genes among sulphonamide-resistant shigella strains isolated in brazil during 1999-2003. | 2005 | 15681578 |
| a novel caspase-1/toll-like receptor 4-independent pathway of cell death induced by cytosolic shigella in infected macrophages. | shigella-induced macrophage cell death is an important step in the induction of acute inflammatory responses that ultimately lead to bacillary dysentery. cell death was previously reported to be dependent upon the activation of caspase-1 via interaction with ipab secreted by intracellular shigella, but in this study, we show that shigella infection of macrophages can also induce cell death independent of caspase-1 or ipab activity. time-lapse imaging and electron microscopic analyses indicated t ... | 2005 | 15695506 |
| a novel shigella dysenteriae serovar isolated in canada. | the etiological agent most commonly associated with bacillary dysentery is shigella. as part of its mandate, the bacteriology and enteric disease program of health canada identifies and serotypes unusual isolates of shigella received from provincial laboratories of public health. in this report, six unusual isolates from three provinces were analyzed biochemically and serologically using slide and tube agglutinations and molecularly using standard pulsed-filed gel electrophoresis (pfge), pcr, an ... | 2005 | 15695673 |
| effects of zinc supplementation as adjunct therapy on the systemic immune responses in shigellosis. | zinc is lost during diarrheal diseases, and zinc deficiency induces intestinal morphology-altering inflammatory responses that zinc supplementation can correct. | 2005 | 15699240 |
| etiology of diarrhea in pediatric outpatient settings. | the frequency with which bacteria cause diarrhea evaluated in ambulatory settings is often unknown. we attempted to determine the microbiologic etiology of diarrhea in a private pediatric practice (site a) and a clinic serving largely immigrant children (site b) and to establish guidelines for bacterial culture. | 2005 | 15702043 |
| serum amyloid a protein binds to outer membrane protein a of gram-negative bacteria. | serum amyloid a (saa) is the major acute phase protein in man and most mammals. we observed saa binding to a surprisingly large number of gram-negative bacteria, including escherichia coli, salmonella typhimurium, shigella flexneri, klebsiella pneumoniae, vibrio cholerae, and pseudomonas aeruginosa. the binding was found to be high affinity and rapid. importantly, this binding was not inhibited by high density lipoprotein with which saa is normally complexed in serum. binding was also observed w ... | 2005 | 15705572 |
| detection of virulence genes in malaysian shigella species by multiplex pcr assay. | in malaysia, shigella spp. was reported to be the third commonest bacterial agent responsible for childhood diarrhoea. currently, isolation of the bacterium and confirmation of the disease by microbiological and biochemical methods remain as the "gold standard". this study aimed to detect the prevalence of four shigella virulence genes present concurrently, in randomly selected malaysian strains via a rapid multiplex pcr (mpcr) assay. | 2005 | 15707504 |
| topological analysis of gtra and gtrb proteins encoded by the serotype-converting cassette of shigella flexneri. | serotype conversion (o-antigen glucosylation) in shigella flexneri is mediated by temperate bacteriophages, which encode a three-gene cluster that contains gtra, gtrb, and gtr([type]). sequence analysis has revealed that gtra and gtrb are conserved and readily interchangeable between serotypes. the gtr([type]) is unique in each serotype and responsible for specifically mediating conversion by the addition of a glucosyl group to the o-antigen units. analysis of the gtra and gtrb amino acid sequen ... | 2005 | 15708010 |