Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| a broad range of human papillomavirus types detected with a general pcr method suitable for analysis of cutaneous tumours and normal skin. | a pair of degenerate pcr primers (fap59/64) was designed from two relatively conserved regions of the l1 open reading frame of most human papillomaviruses (hpv). the size of the generated amplicon was about 480 bp. pcr using these primers was found capable of amplifying dna from 87% (65/75) of the hpv types tested, its sensitivity being 1-10 copies for hpv-5, -20 and -30 clones. hpv was found in 63% (5/8) of tumour samples and in 63% (5/8) of normal skin biopsies from patients with various cutan ... | 1999 | 10501499 |
| transcription-modulatory activities of differentially spliced cdnas encoding the e2 protein of human papillomavirus type 16. | human papillomavirus (hpv) type 16 expresses a variety of alternatively spliced polycistronic mrnas encoding the e2 transcription-regulatory protein. these mrnas initiate at the p97 promoter and contain the 880/2708 (a-type), 880/2581 (a'-type) and 226/2708 (d-type) splice sites upstream from the e2 open reading frame (orf). recent studies investigating the translational capacities of partial cdnas representing three of these mrnas indicated their abilities to function in e2 protein translation, ... | 1999 | 10501502 |
| establishment of the human papillomavirus type 16 (hpv-16) life cycle in an immortalized human foreskin keratinocyte cell line. | the study of human papillomaviruses (hpvs) in cell culture has been hindered because of the difficulty in recreating the three-dimensional structure of the epithelium on which the virus depends to complete its life cycle. additionally, the study of genetic mutations in the hpv genome and its effects on the viral life cycle are difficult using the current method of transfecting molecularly cloned hpv genomes into early-passage human foreskin keratinocytes (hfks) because of the limited life span o ... | 1999 | 10502513 |
| screening for cancer: future potential. | much progress has been made in cancer screening over the past decade, but a great deal more needs to be done if screening is to make a major impact on worldwide cancer mortality. where fully implemented, cytological screening for cervical precursor lesions has had a major impact on mortality. however, the cost and required infrastructure levels are high, and new approaches are needed if screening is to be effective in the developing world. testing for the human papillomavirus and automated liqui ... | 1999 | 10505025 |
| detection of human papillomavirus (hpv) in laryngeal carcinoma cell lines provides evidence for a heterogeneic cell population. | the role of human papillomavirus (hpv) has been studied in laryngeal carcinomas with contradictory results. to evaluate the causal relationship between hpv infection and epithelial malignancies of the larynx, 27 laryngeal carcinoma cell lines from 22 patients were studied. also, paraffin-embedded biopsy samples of the original tumours were available from 12 patients. first, southern blot hybridisation (sbh) was used for the analysis of 18 cell lines and 12 original tumour sections were studied b ... | 1999 | 10505045 |
| the promoter of a novel human papillomavirus (hpv77) associated with skin cancer displays uv responsiveness, which is mediated through a consensus p53 binding sequence. | an aetiological role has been proposed for human papillomavirus (hpv) in skin carcinogenesis within the immunosuppressed patient population. to examine this possibility, we have focused on an hpv type that, to date, has been identified only in the cutaneous lesions of renal transplant recipients despite a high degree of sequence homology with other hpvs commonly found in warts in the general population. we report that the non-coding region of this virus, hpv type 77, contains a consensus binding ... | 1999 | 10508168 |
| molecular analysis of resistance to interferon in patients with laryngeal papillomatosis. | although interferon (ifn)-alpha has been used successfully as an adjuvant therapy in laryngeal papillomatosis, some patients are resistant to this treatment. in order to know which patients will benefit from the therapy, we have tried to find a relationship between the ifn response and the viral and host parameters in the lesion. detection of viral type and copy numbers by polymerase chain reaction (pcr) showed that all patients infected with human papillomavirus (hpv)-11 were sensitive to the t ... | 1999 | 10515680 |
| nasal immunization of mice with human papillomavirus type 16 (hpv-16) virus-like particles or with the hpv-16 l1 gene elicits specific cytotoxic t lymphocytes in vaginal draining lymph nodes. | human papillomavirus type 16 (hpv-16) infects the genital tract and is closely associated with the development of cervical cancer. hpv-16 initiates infection at the genital mucosal surface; thus, mucosal immune responses are likely to contribute to defense against hpv-16 infection. however, little information is available regarding the induction of immune responses in the genital tract mucosa. in this study, we evaluated the potential of intranasally administered papillomavirus vaccines to elici ... | 1999 | 10516012 |
| comparison of variant-specific hybridization and single-strand conformational polymorphism methods for detection of mixed human papillomavirus type 16 variant infections. | pcr-based variant-specific hybridization (vsh) and single-strand conformational polymorphism (sscp) analyses were compared for their capacities to detect mixed human papillomavirus type 16 (hpv-16) variant infections within clinical specimens. the sscp assay used in this comparison targets a 682-bp fragment that spans nucleotides 7445 to 222 within the hpv-16 genome. this fragment includes portions of the hpv-16 long control region and the e6 open reading frame and identifies three categories of ... | 1999 | 10523565 |
| comparative response of normal and of human papillomavirus-16 immortalized human epithelial cervical cells to benzo[a]pyrene. | laboratory evidence suggests synergism of human papillomavirus (hpv) infection with cigarette smoking behaviors in enhancing the risk of cervical cancer. in this preliminary investigation, we tested the hypothesis that hpv infection may alter the metabolic activation of tobacco smoke carcinogens, such as benzo[a]pyrene (b[a]p), thereby playing a role in the etiology of cervical cancer. we examined in vitro the metabolism and dna adduct formation of [3h]b[a]p in normal and hpv-16 immortalized hum ... | 1999 | 10523714 |
| [the role of oncogenic human papillomaviruses in tonsillar squamous cell carcinomas with functional inactivation of the retinoblastoma protein]. | in order to identify squamous cell carcinomas of the head and neck (hnscc) with common biological and clinical features, we investigated the incidence and properties of carcinomas lacking retinoblastoma protein (pr6) cell cycle control. of 208 hnscc investigated, 23 (11%) showed a lack of prb expression. the majority of these tumors (65%) were tonsillar carcinomas. the prb-negative tonsillar tumors were all stage iv, had metastasized to lymph nodes at the time of diagnosis and were in general po ... | 1999 | 10525608 |
| application of homogeneous time-resolved fluorescence (htrftm) to monitor poly-ubiquitination of wild-type p53. | rapid degradation of wild-type p53 in the human uterine cervix is induced by the infection of high-risk human papilloma virus (hpv) types 16 and 18. hpv-e6 protein plays a critical role in the poly-ubiquitination of wild-type p53 by mediating the association of p53 with e6-associated protein (e6ap). as a result, the poly-ubiquitinated p53 is rapidly and selectively degraded by the 26s proteasome. we have established a high throughput assay system to monitor poly-ubiquitination of wild-type p53 u ... | 1999 | 10539989 |
| aetiological parallel between tonsillar and anogenital squamous-cell carcinomas. | patients with human papillomavirus (hpv)-associated anogenital cancers had a 4.3-fold increased risk of tonsillar squamous-cell carcinoma. these cancer types also have histopathological and molecular biological similarities. thus hpv may be aetiologically important in tonsillar carcinogenesis. | 1999 | 10543674 |
| hpv 16 e6 blocks tnf-mediated apoptosis in mouse fibroblast lm cells. | the interaction between hosts and the viruses that infect them is a dynamic one, and a growing literature documents the fact that many viruses have developed mechanisms designed to avoid elimination by the host immune system. one of the immune strategies used by the host and targeted by virus proteins is apoptosis triggered by the cytokine tumor necrosis factor (tnf). mouse fibroblast lm cells are spontaneously sensitive to tnf. when the wild-type e6 protein from the human papillomavirus type 16 ... | 1999 | 10544129 |
| seroprevalence of human papillomavirus type 16 in pregnant women. | to determine the seroprevalence of and risk factors for human papillomavirus (hpv) type 16 capsid antibodies in a large cohort of pregnant women. | 1999 | 10546705 |
| screening for squamous intraepithelial lesions with fluorescence spectroscopy. | to evaluate the accuracy of fluorescence spectroscopy in screening for squamous intraepithelial lesions (sils) and to compare its performance with that of papanicolaou smear screening, colposcopy, cervicoscopy, cervicography, and human papillomavirus (hpv) testing. | 1999 | 10546779 |
| detection of adeno-associated virus in human semen: does viral infection play a role in the pathogenesis of male infertility? | to evaluate the occurrence of adeno-associated virus (aav) dna and/or human papillomavirus (hpv) dna in the semen of infertile men as a possible factor in the pathogenesis of male infertility. | 1999 | 10560983 |
| genital human papillomavirus infection among women recruited for routine cervical cancer screening or for colposcopy determined by hybrid capture ii and polymerase chain reaction. | the purpose of this study was to evaluate the clinical use of the hybrid capture (hc)-ii system for the detection of human papillomavirus (hpv) dna to identify women at risk of progression to high grade squamous intraepithelial lesions (hgsil) and carcinomas by differentiating low risk (lr) hpv types (6, 11, 42, 43, 44) and high/intermediate risk (hr) hpv types (16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 68). five hundred and ninety-six women were enrolled in the study. among them, 466 atte ... | 1999 | 10565688 |
| pcr-rflp-detected human papilloma virus infection in a group of senegalese women attending an std clinic and identification of a new hpv-68 subtype. | cancer of the cervix is the most common malignant tumor among women in africa and, in particular, senegal. studies of the prevalence of human papilloma virus (hpv) infection in africa have mainly focused on carcinomas. data on the presence of the virus in women with normal cervical cytology are scarce. in this study, 158 cytologically normal women who had been referred to the 'institut pasteur de dakar' (senegal) for various genital complaints were investigated for the presence of hpv on exfolia ... | 1999 | 10567840 |
| favorable clinical outcome of cervical cancers infected with human papilloma virus type 58 and related types. | to determine whether the status of human-papillomavirus (hpv) infection affects the clinical outcome of cervical carcinoma (cc), hpv genotype was prospectively determined in 94 consecutive cc cases subsequently followed for a median duration of 37.5 months. with a consensus pcr-rflp method of hpv genotyping, 81 (86.2%) cancers were positive for hpv dna. they were classified, according to the phylogenic similarities, into hpv-16-related (type 16, n = 45; type 31, n = 2), hpv-58-related (type 58, ... | 1999 | 10567897 |
| penile cancer among patients with genital lichen sclerosus. | genital lichen sclerosus (ls) has sporadically been reported to be associated with penile squamous cell carcinoma (scc). | 1999 | 10570372 |
| low levels of serum vitamins a and e in blood and subsequent risk for cervical cancer: interaction with hpv seropositivity. | nutritional factors have been associated with risk of cervical cancer, but it is unclear whether the associations are of etiological significance or secondary to human papillomavirus (hpv) exposure. a delineation of this question requires a prospective study with invasive cancer as the end point. we conducted a nested case-control study in finland and sweden within a joint cohort of 405,000 women followed up for, on average, 4 years. blood samples from 38 prospective cases of invasive cervical c ... | 1999 | 10578492 |
| serological evidence for protection by human papillomavirus (hpv) type 6 infection against hpv type 16 cervical carcinogenesis. | human papillomavirus (hpv) exists as more than 100 genotypes. it is not well-established whether the different hpv types interfere with infection or pathogenesis by each other. possible interactions in cervical carcinogenesis between infection with the most common hpv types (6, 11, 16, 18 and 33) were studied in a seroepidemiological case- control study of 218 women with primary untreated cervical cancer and 219 healthy age-matched control women. as previously shown, hpv-16 seropositivity was as ... | 1999 | 10580926 |
| serum carotenoids and vitamins and risk of cervical dysplasia from a case-control study in japan. | the relationships between risk of cervical dysplasia and dietary and serum carotenoids and vitamins were investigated in a case-control study. cases were 156 women who attended papanicolaou test screening in nine institutes affiliated with japan study group of human papillomavirus (hpv) and cervical cancer and had cervical dysplasia newly histologically confirmed. age-matched controls were selected from women with normal cervical cytology attending the same clinic. blood sample and cervical exfo ... | 1999 | 10584887 |
| magnesium ions enhance the transfer of human papillomavirus e2 protein from non-specific to specific binding sites. | the human papillomavirus 16 e2 protein binds to four specific dna sequences present within the hpv 16 genome and regulates viral gene expression and dna replication. however, the e2 protein can also bind tightly to non-specific dna sequences. here, we show that in binding reactions which contain an excess of non-specific dna, magnesium ions enhance the binding of e2 to its specific sites. in contrast, in the absence of non-specific dna, magnesium ions have no effect on the binding of e2 to speci ... | 1999 | 10588894 |
| the human papillomavirus type 16 e5 protein modulates phospholipase c-gamma-1 activity and phosphatidyl inositol turnover in mouse fibroblasts. | the human papillomavirus type 16 e5 (hpv16-e5) protein is a membrane protein that has been associated with malignant growth. the protein affects growth factor-mediated signal transduction in a ligand-dependent manner. we show now that e5 expression in a31 fibroblasts results in an increased level of diacylglycerol (dag) and inositol phosphates. immunoprecipitation of phospholipase c-gamma-1 (plc-gamma-1) with specific antibodies and immunoblotting with anti-phosphotyrosine antibodies reveal a la ... | 1999 | 10597278 |
| impaired nucleotide excision repair in uv-irradiated human oral keratinocytes immortalized with type 16 human papillomavirus genome. | we previously reported that 'high risk' human papillomaviruses (hpv) induce genetic instability in human oral keratinocytes. to understand the mechanisms of hpv-induced genetic instability, we determined the nucleotide excision repair (ner) capacity of normal (nhok) and human papillomavirus type-16 immortalized oral keratinocytes (hok-16b) by strand-specific removal of uv-induced cyclobutane pyrimidine dimers (cpds) from a 16 kb fragment of the p53 gene. in nhok the ner activity was initiated in ... | 1999 | 10597299 |
| synthesis of ifn-gamma by cd8(+) t cells is preserved in hiv-infected women with hpv-related cervical squamous intraepithelial lesions. | the aim of this study was to investigate whether coinfection with hiv affects the synthesis of th1 and th2 cytokines by peripheral blood t cells of women infected with human papillomavirus (hpv). | 1999 | 10600293 |
| naturally occurring, nonregressing canine oral papillomavirus infection: host immunity, virus characterization, and experimental infection. | papillomaviruses occasionally cause severe, nonregressing or recurrent infections in their human and animal hosts. the mechanisms underlying these atypical infections are not known. canine oral papillomavirus (copv) typically regresses spontaneously and is an important model of mucosal human papillomavirus infections. a severe, naturally occurring, nonregressing copv infection provided an opportunity to investigate some aspects of viral pathogenicity and host immunity. in this case, the papillom ... | 1999 | 10600607 |
| human papillomavirus and the risk of cervical cancer. | human papillomavirus (hpv) is now recognized as the driving force behind the dysregulation of the cell cycle that underlies cervical malignant transformation. although nearly all cervical cancers contain hpv genomes, the vast majority of hpvs are not oncogenic but merely induce benign lesions. because progressive abnormalities take at least a decade to develop into invasive cancers, young women with low-grade lesions can often be managed with watchful waiting rather than ablative therapy. | 1999 | 10616548 |
| dwarfism and dysregulated proliferation in mice overexpressing the myc antagonist mad1. | the four members of the mad family are bhlhzip proteins that heterodimerize with max and act as transcriptional repressors. the switch from myc-max complexes to mad-max complexes has been postulated to couple cell-cycle arrest with differentiation. the ectopic expression of mad1 in transgenic mice led to early postnatal lethality and dwarfism and had a profound inhibitory effect on the proliferation of the hematopoietic cells and embryonic fibroblasts derived from these animals. compared to wild ... | 1999 | 10616903 |
| new method for automatic identification identification and typing of single and multiple superimposed human papillomavirus sequences. | this study of specimens of human papillomaviruses (hpv) through hpv-specific polymerase chain reaction (pcr), followed by direct sequencing, resulted in 11% (38/354) superimposed hpv sequences, signifying coinfection with more than one hpv type. to address the diagnostic problem that these superimposed ("degenerated," overlapping) sequences pose, the authors created a papillomavirus database in microsoft excel (microsoft corporation, redmond, wa, u.s.a.) and corel quattro pro 9 (corel corporatio ... | 1999 | 10617279 |
| [verification of identification of infectious agents by culture in routine gynecologic cytological screening]. | the original bethesda classification system for reporting cervical/vaginal cytologic diagnoses has claimed besides oncologic evaluation also a statement on the presence of infectious agents. their diagnosis should be followed by appropriate treatment. based upon the comparison of careful bacterioscopic study in a series of 175 routine cervical smears with the results of microbiological, virological and mycological examinations the following pathogens might be-according to the authors' opinion-di ... | 1999 | 11038666 |
| transforming growth factor beta 1 (tgf beta 1) down-regulates expression and function of proliferation-inducing molecules in hpv-transformed cells. | the epidemiologic association of human papillomavirus (hpv) infection with dysplasia and cervical cancer is well established. transforming growth factor beta 1 (tgf beta 1) is a growth inhibitory protein for epithelial cells. to examine the phenotype of hpv-transformed cells, we examined expression of tgf beta 1 and a number of cellular proliferation-enhancing molecules which are known to be regulated by tgf beta 1, including bcl-2, c-jun and nfkb. previous studies had identified significant ind ... | 1999 | 10697499 |
| absence of human papillomavirus sequences in early stage prostate cancer. | an etiological role for human papilloma viruses (hpvs) has been proposed for a variety of malignancies, and especially in cervical carcinomas. the role of hpvs in prostate cancer remains controversial. in particular, using primers derived from the l1 gene of hpv, it has been suggested that hpv sequences are associated with prostate malignancies. pcr and southern blot hybridization was used to detect the presence of hpv dna in fresh tissue from patients undergoing radical prostatectomy for prosta ... | 1999 | 11180776 |
| dna vaccine against oncogenic hamster cells transformed by hpv16 e6/e7 oncogenes and the activated ras oncogene. | the capability of dna to elicit anti-tumour immunity was studied using human papillomavirus type 16 (hpv16)-transformed syrian hamster cells denoted k3/ii. these cells had been derived after cotransfection of primary kidney cell cultures with p16hhmo plasmid containing e6/e7 oncogenes of hpv16 and pej6.6 plasmid containing the activated human h-ras oncogene; they express both the hpv16 and activated h-ras genes. as a dna vaccine, the p16hhmo plasmid was used. three doses of the plasmid (either 1 ... | 1999 | 9864430 |
| histologic and immunologic associations of an hpv16 variant in losil smears. | human papillomavirus type 16 variants have been described and may have different biologic activities: this has implications for the design of hpv vaccines. | 1999 | 9889030 |
| two polymorphic variants of wild-type p53 differ biochemically and biologically. | the wild-type p53 protein exhibits a common polymorphism at amino acid 72, resulting in either a proline residue (p53pro) or an arginine residue (p53arg) at this position. despite the difference that this change makes in the primary structure of the protein resulting in a difference in migration during sodium dodecyl sulfate-polyacrylamide gel electrophoresis, no differences in the biochemical or biological characteristics of these wild-type p53 variants have been reported. we have recently show ... | 1999 | 9891044 |
| the angelman syndrome-associated protein, e6-ap, is a coactivator for the nuclear hormone receptor superfamily. | in this study, we found that the e6-associated protein (e6-ap/ube3a) directly interacts with and coactivates the transcriptional activity of the human progesterone receptor (pr) in a hormone-dependent manner. e6-ap also coactivates the hormone-dependent transcriptional activities of the other members of the nuclear hormone receptor superfamily. previously, it was shown that e6-ap serves the role of a ubiquitin-protein ligase (e3) in the presence of the e6 protein from human papillomavirus types ... | 1999 | 9891052 |
| differential expression of immunobiological mediators by immortalized human cervical and vaginal epithelial cells. | we have recently generated human papillomavirus (hpv) 16/e6e7 immortalized epithelial cell lines from the human vagina, ectocervix, and endocervix to use in studies on the role of these cells in reproduction and immune defense. the cell lines maintain the differentiation characteristics of their tissues of origin: the endocervical cell line expresses characteristics of simple columnar epithelium, whereas the ectocervical and vaginal cell lines express characteristics of stratified squamous nonke ... | 1999 | 9916021 |
| relationship between human papillomavirus type 16 in the cervix and intraepithelial neoplasia. | to evaluate a temporal relationship between the presence of cervical human papilloma virus (hpv) type 16 and the risk of developing cervical intraepithelial neoplasia (cin). | 1999 | 9916955 |
| laser conization of cervical intraepithelial neoplasia grade 3: free resection margins indicative of lesion-free survival. | various grades of cervical intraepithelial neoplasia may occur following laser conization for grade 3 lesions. the aim of this study was to assess lesion-free survival after laser conization in cases with/without free resection margins, and to test whether detection of human papillomavirus infection and/or p53 expression in the cone lesion were useful predictors of lesion-free survival. | 1999 | 9926893 |
| photodynamic therapy sensitivity is not altered in human tumor cells after abrogation of p53 function. | photodynamic therapy (pdt) is an effective local cancer treatment that induces cytotoxicity through the intracellular generation of reactive oxygen species. the current study investigated whether abrogation of wild-type p53 expression modified the sensitivity of tumor cells to pdt-mediated oxidative stress. in these experiments, human colon (ls513) and breast (mcf-7) carcinoma cells exhibiting a wild-type p53 phenotype were directly compared to ls513 and mcf-7 cells with abrogated p53 function i ... | 1999 | 9927042 |
| prediction of recurrent and residual cervical dysplasia by human papillomavirus detection among patients with abnormal cytology. | to determine the discriminative capacity of human papillomavirus (hpv) dna testing for recurrent and residual cervical dysplasia, 43 patients with abnormal cytology after treatment for cervical dysplasia were tested for the presence of hpv dna by pcr. an endocervical curettage was performed in all patients for histological examination. sixteen of the 43 patients showed moderate or severe dysplasia. the hpv test was positive in all 16 patients with recurrent or residual dysplasia and negative in ... | 1999 | 10021301 |
| combination therapy with podophyllin and vidarabine for human papillomavirus positive cervical intraepithelial neoplasia. | our aim was to establish an effective non-surgical treatment for cervical intraepithelial neoplasia (cin) through inactivation of human papillomavirus (hpv), the major etiological agent for this disease. we show that vidarabine, a dna polymerase inhibitor, suppressed growth and hpv gene expression in human cervical keratinocytes immortalized by hpv or in cervical cancer cell lines. expression of hpv-16 e6 and e7 proteins in normal cervical keratinocytes sensitized cells to apoptosis in the prese ... | 1999 | 10022988 |
| evaluation of human papillomavirus-consensus primers for hpv detection by the polymerase chain reaction. | cervical cancer is one of the most frequently found cancers in women and appears to have a viral aetiology. substantial evidence points to the human papillomaviruses (hpv) as the infectious agents and there is considerable interest in identifying and accurately typing the viruses. since hpvs now comprise more than 100 different hpv types, the polymerase chain reaction (pcr) has been the preferred methodology for virus identification and typing on isolated dna. in that context, five commonly empl ... | 1999 | 10024428 |
| a possible involvement of aberrant expression of the fhit gene in the carcinogenesis of squamous cell carcinoma of the uterine cervix. | to investigate involvement of an aberrant expression of the fhit (fragile histidine triad) gene in the process of carcinogenesis and progression in cervical carcinoma, we examined its expression by the reverse transcriptase polymerase chain reaction (rt-pcr) and cdna sequence method in 32 cervical invasive carcinomas (25 squamous cell carcinomas and seven adeno- or adenosquamous carcinomas) and 18 of its precursor lesions [four low-grade and 14 high-grade cervical intraepithelial neoplasias (cin ... | 1999 | 10027335 |
| cervicovaginal human papillomavirus infection in human immunodeficiency virus-1 (hiv)-positive and high-risk hiv-negative women. | human papillomavirus (hpv) infection is associated with precancerous cervical squamous intraepithelial lesions commonly seen among women infected with human immunodeficiency virus-1 (hiv). we characterized hpv infection in a large cohort of hiv-positive and hiv-negative women participating in the women's interagency hiv study to determine the prevalence of and risk factors for cervicovaginal hpv infection in hiv-positive women. | 1999 | 10037100 |
| the psychosexual impact of human papillomavirus cervical infections. | occult human papillomavirus (hpv) infection, present in approximately 20% of women in the united states, is usually sexually transmitted, associated with substantial health risks, and unpredictable in its resolution. the potential for adverse psychosexual alterations due to hpv infection in women considered at low risk for bacterial sexually transmitted diseases is substantial, but data is lacking. | 1999 | 10037541 |
| two posttranscriptional pathways that regulate p21(cip1/waf1/sdi1) are identified by hpv16-e6 interaction and correlate with life span and cellular senescence. | the p21((cip1/waf1/sdi1)) protein is a cyclin-dependent kinase inhibitor that is induced in normal human fibroblasts (nhf) following dna damage, following serum stimulation, and at cellular senescence. expression of the human papilloma virus 16 e6 oncoprotein in nhf cells results in the loss of the p21 protein, independent of mrna level under most conditions. the p21 protein levels in nhf-e6 cells remained low following dna damage or serum stimulation even though mrna levels increased. in contra ... | 1999 | 10047459 |
| the introduction of dominant-negative p53 mutants suppresses temperature shift-induced senescence in immortal human fibroblasts expressing a thermolabile sv40 large t antigen. | immortal human fibroblasts, svts8 cells, which express a heat-labile sv40 large t antigen, induces a senescence-like phenomenon in response to upward shift in temperature. cells with arrested division show strong induction of senescence-associated beta-galactosidase. we examined how p53 and prb are involved in this phenomenon since they are major targets of the t antigen. transfection of cells with plasmids encoding the wild-type t antigen or human papilloma virus type 16 e6/e7 proteins complete ... | 1999 | 10050041 |
| human papilloma virus type 16 e6 gene expression in oral exophytic epithelial lesions as detected by in situ rtpcr. | we adopted an in situ reverse transcriptase polymerase chain reaction method of detecting and determining the frequency of early (e6) gene expression of human papilloma virus type 16 at the individual cell level in a sample of oral exophytic lesions with various degrees of epithelial hyperplasia and dysplasia in immunosuppressed and immunocompetent patients. | 1999 | 10052376 |
| risk factors for abnormal anal cytology in young heterosexual women. | although anal cancers are up to four times more common in women than men, little is known about the natural history of anal human papillomavirus (hpv) infections and hpv-related anal lesions in women. this study reports on the prevalence of and risks for anal cytological abnormalities over a 1-year period in a cohort of young women participating in a study of the natural history of cervical hpv infection. in addition to their regularly scheduled sexual behavior interviews and cervical testing, c ... | 1999 | 10067816 |
| immune responses against human papillomavirus (hpv) type 16 virus-like particles in a cohort study of women with cervical intraepithelial neoplasia. ii. systemic but not local iga responses correlate with clearance of hpv-16. | to investigate whether there is an association between local or systemic igg and iga responses against human papillomavirus (hpv) type 16 virus-like particles (vlp) containing l1 and l2 and the possible influence of these responses on clearance of hpv-16 and its associated lesions, cervical mucus samples from 125 patients and plasma samples from 100 patients, all participating in a non-intervention cohort study of women with abnormal cytology, were analysed. the results show that local igg and i ... | 1999 | 10073701 |
| effects of yokuinin on the therapeutic efficacy of a new benzoxazinorifamycin krm-1648 against mycobacterium avium infection. | the chinese traditional medicine, yokuinin, which has anti-inflammatory effects and anti-human papilloma virus activity, was examined for its effects on the therapeutic efficacy of a benzoxazinorifamycin krm-1648 (krm) against myobacterium avium infection in mice. reverse transcription-pcr analysis revealed that yokuinin increased the mrna expression of all test cytokines in lung tissues of infected ice at week 8, in the order transforming growth factor-beta (tgf-beta) > ifn-gamma > tnf-alpha > ... | 1999 | 10075281 |
| kinetic selection of hpv 16 e6/e7-directed antisense nucleic acids: anti-proliferative effects on hpv 16-transformed cells. | the e6/e7-coding sequences of the human papillomavirus type 16 (hpv 16) were probed for kinetic accessibility in vitro by pools of catalytic antisense rna. only long-chain complementary rna and very few antisense sequences with a 3' portion complementary to a 10 nt window within unspliced and spliced e6-coding target sequences showed fast annealing with k(ass) values of up to 10(4) m-1s-1 indicating that the majority of e6/e7 rna sequences are unfavourable targets for antisense inhibitors and ri ... | 1999 | 10075988 |
| induction of tumor antigen-specific immunity using plasmid dna immunization in mice. | we have evaluated the ability of bioballistic "gene gun" immunization of mice with plasmid dna encoding clinically relevant tumor antigens to induce protective antitumor immunity. mice immunized with plasmid cdna encoding the cervical carcinoma-associated human papillomavirus 16-e7 gene product exhibited potent anti-e7-specific cytotoxic t lymphocytes and were protected completely against a subsequent challenge with the e7+ c3 sarcoma. of perhaps greater clinical interest, genetic immunization u ... | 1999 | 10078966 |
| colonization of in vitro-formed cervical human papillomavirus- associated (pre)neoplastic lesions with dendritic cells: role of granulocyte/macrophage colony-stimulating factor. | the purpose of this study was to investigate the ability of cd1a+ langerhans/dendritic cells (lcs/dcs) to infiltrate human papillomavirus (hpv)-associated (pre)neoplastic lesions of the uterine cervix. migration of lcs/dcs in the presence of keratinocytes derived from normal cervix and hpv-transformed cell lines was evaluated in boyden chambers and in organotypic cultures and correlated with granulocyte/macrophage colony-stimulating factor (gm-csf) production by the cells, as determined by elisa ... | 1999 | 10079255 |
| synergism with germ line transcription factor oct-4: viral oncoproteins share the ability to mimic a stem cell-specific activity. | activation of transcription by oct-4 from remote binding sites requires a cofactor that is restricted to embryonal stem cells. the adenovirus e1a protein can mimic the activity of this stem cell-specific factor and stimulates oct-4 activity in differentiated cells. here we characterize the oct-4-e1a interaction and show that the e1a 289r protein harbors two independent oct-4 binding sites, both of which specifically interact with the pou domain of oct-4. furthermore, we demonstrate that, like e1 ... | 1999 | 10082529 |
| control of replicative life span in human cells: barriers to clonal expansion intermediate between m1 senescence and m2 crisis. | the accumulation of genetic abnormalities in a developing tumor is driven, at least in part, by the need to overcome inherent restraints on the replicative life span of human cells, two of which-senescence (m1) and crisis (m2)-have been well characterized. here we describe additional barriers to clonal expansion (mint) intermediate between m1 and m2, revealed by abrogation of tumor-suppressor gene (tsg) pathways by individual human papillomavirus type 16 (hpv16) proteins. in human fibroblasts, a ... | 1999 | 10082577 |
| [virologic aspects of juvenile laryngeal papillomatosis]. | juvenile laryngeal papillomatosis is the most common benign tumor of the larynx in childhood. the specific etiological factors are non-oncogenic human papillomavirus types 6 and 11. in the present study two cases (a 6-year-old male and a 5 and a half-year-old female) operated five times each and harbouring type 11 dna in papillomas excised in the first operations are analysed from the following virological aspects: 1. the examination of vertical transmission by general primer-polymerase chain re ... | 1999 | 10083813 |
| overexpression of d-type cyclins, e2f-1, sv40 large t antigen and hpv16 e7 rescue cell cycle arrest of tsbn462 cells caused by the ccg1/taf(ii)250 mutation. | tsbn462 cells, which have a point mutation in ccg1/taf(ii)250, a component of tfiid complex, arrest in g1 at the nonpermissive temperature of 39.5 degrees c. overexpression of d-type cyclins rescued the cell cycle arrest of tsbn462 cells, suggesting that the cell cycle arrest was through rb. consistent with this, overexpression of e2f-1, whose function is repressed by the hypophosphorylated form of rb, also rescued the cell cycle arrest. moreover, expression of the viral oncoproteins sv40 large ... | 1999 | 10086334 |
| cumulation of tp53 mutations and p16ink4a/p15ink4b homozygous deletions in human papilloma virus type 16 positive scrotal cancer. | scrotal cancer is the first described occupational cancer. the frequency of occupation-related scrotal cancer is very rare because of better hygiene and protective clothing. human papilloma viruses (oncogenic types 16 and 18) were reported as the causative agents in the pathogenesis of scrotal cancers. e5, e6, and e7 proteins, expressed by human papilloma virus type 16, affect the cell cycle at the g1 checkpoint. tp53, p16ink4a, and p15ink4b were reported as the transcription factors that regula ... | 1999 | 10087941 |
| comparison of in-situ hybridization, direct and indirect in-situ pcr as well as tyramide signal amplification for the detection of hpv. | one hundred paraffin-embedded cervical biopsy specimens were tested for the presence of human papilloma virus (hpv) by in situ hybridization (ish), and by direct and indirect in situ pcr (is-pcr) in order to evaluate the efficiency of the different in situ methods in detecting hpv infection. ish was performed using either commercial dna probes or a cocktail of 5'-digoxigenin labeled oligoprimers. the same were used for ish during indirect is-pcr. to enhance the sensitivity of ish several polymer ... | 1999 | 10090569 |
| [natural course of hpv infection. usefulness of hpv analysis in cervix diagnosis]. | cervical carcinomas and their precursors (cervical dysplasia, cin1-3) are associated with human papillomavirus (hpv) infections. epidemiological and in vitro-studies have shown that some of the genital hpv types, the high risk-types 16, 18, 31 etc., code for proteins (e6/e7) which strongly influence the cell cycle and genome stability. progression from weak to severe dysplasia and to invasive cancer is associated with increasing expression of these viral oncogenes. which additional cofactors con ... | 1999 | 10091228 |
| a survey of seroprevalence of human papillomavirus types 16, 18 and 33 among children. | the importance and natural history of hpv infections in childhood is incompletely understood. we performed a survey for presence of serum antibodies to hpv capsids among 1031 children aged 0 to 13 years, resident in stockholm, sweden. the hpv seroprevalence among these children was 3.0% for hpv16, 0.6% for hpv18 and 2.7% for hpv33. by comparison, among simultaneously analyzed positive control panels comprising women with cin or healthy women with type-specific cervical hpv dna, seroprevalence of ... | 1999 | 9935145 |
| hpv 16 antibody prevalence in jamaica and the united states reflects differences in cervical cancer rates. | human papillomavirus (hpv) is widely accepted as the primary etiologic agent in the development of cervical cancer. dna of a particular hpv type, hpv 16, is found in about half of tumors tested. inconsistent with this causal relationship, however, population-based studies of hpv dna prevalence have often failed to find high rates of anogenital hpv infection in countries with high cervical cancer rates. to examine this issue, we used serology to compare hpv 16 exposure in healthy volunteer blood ... | 1999 | 9935171 |
| the chromatin structure of the long control region of human papillomavirus type 16 represses viral oncoprotein expression. | the long control region (lcr) of human papillomavirus type 16 (hpv-16) has a size of 850 bp (about 12% of the viral genome) and regulates transcription and replication of the viral dna. the 5' segment of the lcr contains transcription termination signals and a nuclear matrix attachment region, the central segment contains an epithelial cell-specific enhancer, and the 3' segment contains the replication origin and the e6 promoter. here we report observations on the chromatin organization of this ... | 1999 | 9971771 |
| detection and quantitation of human papillomavirus by using the fluorescent 5' exonuclease assay. | a method for the detection and quantitation of oncogenic human papillomavirus (hpv) was developed by using the fluorescent 5' exonuclease assay. the method is based on the amplification of a 180-bp fragment from the 3' part of the e1 open reading frame in a single pcr with type-specific probes for hpv types 16, 18, 31, 33, and 35. the probes can be used separately or in combinations of up to three probes per assay. quantitation over a range of 10(1) to 10(6) initial hpv copies was possible by us ... | 1999 | 9986801 |
| differential gene expression in neoplastic and human papillomavirus-immortalized oral keratinocytes. | we have previously demonstrated that normal human oral keratinocytes immortalized by transfection with human papillomavirus type-16 dna became tumorigenic after exposure to a chemical carcinogen. in an effort to detect differentially regulated genes associated with this transition from the immortal to the malignant phenotype, we employed representational differences analysis (a pcr-coupled subtractive hybridization technique). after analysing 50 colonies, 12 putative messages were identified. no ... | 1999 | 9989835 |
| modulation of type m2 pyruvate kinase activity by the human papillomavirus type 16 e7 oncoprotein. | we report here that the e7 oncoprotein encoded by the oncogenic human papillomavirus (hpv) type 16 binds to the glycolytic enzyme type m2 pyruvate kinase (m2-pk). m2-pk occurs in a tetrameric form with a high affinity to its substrate phosphoenolpyruvate and a dimeric form with a low affinity to phosphoenolpyruvate, and the transition between both conformations regulates the glycolytic flux in tumor cells. the glycolytic intermediate fructose 1, 6-bisphosphate induces the reassociation of the di ... | 1999 | 9990017 |
| expression of the e6 and e7 genes of human papillomavirus (hpv16) extends the life span of human myoblasts. | primary human myoblasts (satellite cells), like other human cells, have a limited life span in vitro. here we show that expression of the e6e7 early region from human papillomavirus type 16 can greatly extend the life span of both fetal and satellite cell-derived myoblasts and release them from dependence on the growth factors normally necessary for their proliferation. expression of either the e6 or the e7 gene alone was not sufficient to confer this phenotype, although expression of e7 did del ... | 1999 | 10094825 |
| dna replication specificity and functional e2 interaction of the e1 proteins of human papillomavirus types 1a and 18 are determined by their carboxyl-terminal halves. | replication of most papillomaviruses (pvs) requires the viral-encoded e1 and e2 proteins that bind to the origin of replication (ori) containing the e1- and e2-binding sites and help recruit host replication factors during the initiation of dna replication. we studied the ability of heterologous e1 and e2 proteins to interact in vivo and support replication, using the human papillomavirus (hpv) types 1a and 18 as model systems. the e1 protein of hpv-1a in combination with hpv-18 e2 supported hig ... | 1999 | 10191198 |
| ccaat displacement protein binds to and negatively regulates human papillomavirus type 6 e6, e7, and e1 promoters. | expression of human papillomavirus genes increases as the target cell, the keratinocyte, differentiates. ccaat displacement protein (cdp) is a cellular protein which has been shown in other cell types to negatively regulate gene expression in undifferentiated cells but not in differentiated cells. we have previously shown that a 66-bp purine-thymidine-rich sequence (the 66-mer) binds cdp and negatively regulates the human papillomavirus type 6 (hpv-6) e6 promoter (s. pattison, d. g. skalnik, and ... | 1999 | 10196318 |
| biological behavior and etiology of inflammatory cervical smears. | two hundred and fifty-seven consecutive women attending a major maternal and child health (mch) center were studied clinically, colposcopically, cytologically, and microbiologically for different gynecologic infections. out of 257 cases, 207 (80.5%) had inflammatory cervical smears, of which 183 (88.4%) were infected with one or more genital tract infections. bacterial vaginosis (risk, 22.6-fold), chlamydia (risk, 21.6-fold), and human papillomavirus (hpv) (risk, 13.5-fold) were independently as ... | 1999 | 10204101 |
| a human papillomavirus e2 transcriptional activator. the interactions with cellular splicing factors and potential function in pre-mrna processing. | the human papillomavirus (hpv) e2 protein plays an important role in transcriptional regulation of viral genes as well as in viral dna replication. unlike most types of hpv, the e2 protein of epidermodysplasia verruciformis (ev)-associated hpvs harbors a relatively long hinged region between the terminal, conserved transactivation and dna binding/dimerization domains. the sequence of ev-hpv e2 hinge contains multiple arginine/serine (rs) dipeptide repeats which are characteristic of a family of ... | 1999 | 10207001 |
| an unusual cervical carcinoma showing exception to epitheliotropism of human papillomavirus. | human papillomaviruses (hpv) infect epithelial tissues but have not been previously detected within mesenchymal cells. during a systematic investigation of figo stage ib cervical cancers with colorimetric in situ hybridization, we detected hpv 16 dna within the stromal compartment of an unusual undifferentiated carcinoma. the mesenchymal nature of the hpv-containing cells was confirmed by immunohistochemistry and electron microscopy. no viral particles were identified. sequencing the majority of ... | 1999 | 10208473 |
| the antigen-presenting environment in normal and human papillomavirus (hpv)-related premalignant cervical epithelium. | the activation of hpv-specific t cells within the cervical microenvironment is likely to play an important part in the natural history of cervical intraepithelial neoplasia (cin). the extent and the type of t cell activation will depend critically on the expression of mhc, costimulatory cell surface molecules and cytokines by keratinocytes and langerhans cells within the cervical lesion. expression of mhc class ii (hla-a-dr and -dq), costimulatory/adhesion molecules (cd11a/18, cd50, cd54, cd58 a ... | 1999 | 10209502 |
| smoking and oral contraceptives as risk factors for cervical carcinoma in situ. | human papillomavirus (hpv) is probably a necessary but definitely not a sufficient cause of cervical carcinoma. however, it remains unclear which factors, in addition to hpv, are important for the development of cervical carcinoma and its precursor lesions. to address this issue, we conducted a case-control study nested in a population-based cohort consisting of women participating in cytological screening in one swedish county, any time during 1969 through 1995. detailed information on sexual p ... | 1999 | 10209949 |
| interleukin-12-secreting human papillomavirus type 16-transformed cells provide a potent cancer vaccine that generates e7-directed immunity. | the development of a vaccine that would be capable of preventing or curing the (pre)cancerous lesions induced by genital oncogenic human papillomaviruses (hpvs) is the focus of much research. many studies are presently evaluating vaccines based on the viral e6 and e7 oncoproteins, both of which are continually expressed by tumor cells. the success of a cancer vaccine relies, in large part, on the induction of a tumor-specific th1-type immunity. in this study, we have evaluated the ability of b7- ... | 1999 | 10209958 |
| human papillomavirus and prognoses of patients with cancers of the upper aerodigestive tract. | some studies have shown that human papillomavirus (hpv) infection may play not only an etiologic role in anogenital cancers but also a role in the clinical outcome. the objective of the current study was to determine whether detection of hpv dna in primary squamous cell carcinomas of the upper aerodigestive tract (uadt) is a prognostic factor in patients with the disease. | 1999 | 10223228 |
| interlaboratory agreement of different human papillomavirus dna detection and typing assays in cervical scrapes. | 1999 | 10225460 | |
| prevalence of human papilloma virus dna in head and neck cancers carrying wild-type or mutant p53 tumor suppressor genes. | the normal function of the p53 tumor suppressor protein can be perturbed by non-mutational mechanisms. the e6 protein encoded by high risk strains of human papilloma virus (hpv) targets the p53 protein resulting in enhanced degradation via the ubiquitin pathway. we have used nested pcr for detecting the presence of hpv dna in 58 primary head and neck tumors and 15 metastatic lymph nodes, which had been prescreened for p53 mutations in exons 5 to 8. hpv dna sequences were detected in 12 tumors (2 ... | 1999 | 10226517 |
| chimeric papillomavirus virus-like particles induce a murine self-antigen-specific protective and therapeutic antitumor immune response. | the use of chimeric virus-like particles represents a new strategy for delivering tumor antigens to the immune system for the initiation of antitumor immune responses. immunization of dba/2 mice with the p1a peptide derived from the p815 tumor-associated antigen p1a induced specific t-cell tolerance, resulting in progression of a regressor p815 cell line in all animals. however, immunization with a human papillomavirus type 16 l1 virus-like particle containing the p1a peptide in the absence of a ... | 1999 | 10227378 |
| human papillomavirus types 16 and 39 in a vulval carcinoma occurring in a woman with hailey-hailey disease. | a woman with hailey-hailey disease, suffering from carcinoma of the vulva, was examined by histology and for the presence of human papillomavirus (hpv) dna by polymerase chain reaction (pcr) and in situ hybridization. our diagnosis by histological examination revealed the vulval carcinoma to be a squamous cell carcinoma (scc), adjacent to lesions of hailey-hailey disease and severe dysplasia/carcinoma in situ [vulval intraepithelial neoplasia (vin) iii]. the pcr with consensus primers for the l1 ... | 1999 | 10233276 |
| allelic loss in human papillomavirus-positive and -negative vulvar squamous cell carcinomas. | vulvar squamous cell carcinoma (vscc) is a biologically and morphologically diverse disease, consisting of human papillomavirus (hpv)-positive and -negative tumors that differ in their morphological phenotypes and associated vulvar mucosal disorders. this study analyzed the frequencies of allelic loss (loss of heterozygosity (loh)) in hpv-positive and -negative vsccs to identify potential targets for the study of preinvasive diseases, to determine whether hpv status influenced patterns of loh, a ... | 1999 | 10233839 |
| characterization of a major neutralizing epitope on human papillomavirus type 16 l1. | persistent infection with human papillomavirus type 16 (hpv-16) is strongly associated with the development of cervical cancer. neutralizing epitopes present on the major coat protein, l1, have not been well characterized, although three neutralizing monoclonal antibodies (mabs) had been identified by using hpv-16 pseudovirions (r. b. roden et al., j. virol. 71:6247-6252, 1997). here, two of these mabs (h16.v5 and h16.e70) were demonstrated to neutralize authentic hpv-16 in vitro, while the thir ... | 1999 | 10233949 |
| trichostatin a up-regulates human papillomavirus type 11 upstream regulatory region-e6 promoter activity in undifferentiated primary human keratinocytes. | human papillomavirus (hpv) gene expression in squamous epithelia is differentiation dependent in benign patient lesions and in organotypic raft cultures of primary human keratinocytes (phks). using the lacz reporter in raft cultures, we previously showed that this transcriptional regulation of the hpv type 11 (hpv-11) enhancer-promoter located in the upstream regulatory region (urr) appears to have resulted from coordination between the transcription transactivators ap1, oct1, and sp1 in differe ... | 1999 | 10233965 |
| identifying women with cervical neoplasia: using human papillomavirus dna testing for equivocal papanicolaou results. | a papanicolaou (pap) test result of atypical squamous cells of undetermined significance (ascus) presents a clinical challenge. only 5% to 10% of women with ascus harbor serious cervical disease, but more than one third of the high-grade squamous intraepithelial lesions (hsils) in screening populations are identified from ascus pap test results. | 1999 | 10235153 |
| resolution of recalcitrant hand warts in an hiv-infected patient treated with potent antiretroviral therapy. | human papilloma virus (hpv)-related cutaneous manifestations occur with increased frequency and severity among hiv-infected persons. in this report, we describe an hiv-infected man with persistent, severe cutaneous hand warts that did not respond to multiple therapies, including liquid nitrogen cryotherapy, topical dinitrochlorobenzene, topical podophyllin, and intralesional interferon-alfa injections. approximately 1 year after starting a potent protease inhibitor-containing antiretroviral regi ... | 1999 | 10321624 |
| induction of the p53-target gene gadd45 in hpv-positive cancer cells. | the e6 oncoprotein of human papillomaviruses (hpvs) has the potential to functionally antagonize p53. in several experimental model systems, ectopic expression of e6 can block the genotoxic induction of the growth inhibitory p53 target gene gadd45, suggesting that the inactivation of this pathway may play a major role for hpv-associated cell transformation. here, we investigated whether this reflects the regulation of gadd45 expression in carcinoma-derived hpv-positive cells. we found that the g ... | 1999 | 10327059 |
| is human papillomavirus testing of value in clinical practice? | the objective of this review was to evaluate the current status of human papillomavirus testing in predicting the presence of high-grade or invasive disease in the cervix in women with papanicolaou smears reported as atypical squamous cells of undetermined significance (ascus) or low-grade squamous intraepithelial lesion. this is a review of many of the studies already published evaluating the utility of human papillomavirus testing as part of the triage for patients whose papanicolaou smears we ... | 1999 | 10329854 |
| cervical human papillomavirus deoxyribonucleic acid persists throughout pregnancy and decreases in the postpartum period. | our goal was to determine the persistence of human papillomavirus infection of the cervix in a prospectively evaluated cohort of pregnant women observed from the first trimester until after delivery. | 1999 | 10329863 |
| target site selection for an rna-cleaving catalytic dna. | a small catalytic dna, known as the 10-23 dna enzyme or deoxyribozyme, has been shown to efficiently hydrolyze rna at purine-pyrimidine (r-y) junctions in vitro. although these potentially cleavable junctions are ubiquitous, they are often protected from deoxyribozyme activity by rna secondary structure. we have developed a multiplex cleavage assay for screening the entire length of a target rna molecule for deoxyribozyme cleavage sites that are efficient, both in terms of kinetics and accessibi ... | 1999 | 10331809 |
| hpv in situ hybridization with catalyzed signal amplification and polymerase chain reaction in establishing cerebellar metastasis of a cervical carcinoma. | we report an unusual case of cerebellar metastasis from a cervical adenosquamous carcinoma in which molecular techniques assisted in establishing the correct diagnosis. the patient was a 43-year-old woman with surgically unresectable cervical carcinoma diagnosed 2 years before presenting with neurological symptoms. a magnetic resonance imaging scan showed a large, enhancing cerebellar lesion with significant brain stem compression. the excised cerebellar tumor resembled a small cell carcinoma an ... | 1999 | 10333232 |
| thin-layer cytology meets viral hybrid capture. | this commentary addresses the benefits of liquid-based thin-layer cervical cytology, and the applicability of residual samples for human papilloma virus assay. the potential for use of this technology in wide-scale screening as well as the many unanswered issues, in particular cost-benefit ratio and financing, are also discussed. | 1999 | 10342014 |
| comèl-netherton syndrome complicated by papillomatous skin lesions containing human papillomaviruses 51 and 52 and plane warts containing human papillomavirus 16. | we describe a 28-year-old woman with characteristic clinical signs of comèl-netherton syndrome (cns) who showed numerous plane warts on her face and forearms and papillomatous skin tumours affecting her groins and genitoanal skin. using human papillomavirus (hpv) type-specific primers for cutaneous and mucosal hpv types we identified hpv 16-specific sequences in plane warts and hpv 51- and hpv 52-specific dna in papillomatous skin from the patient's groins, suggesting a pathogenetic role (cofact ... | 1999 | 10354085 |
| induction of human papillomavirus-specific cd4(+) and cd8(+) lymphocytes by e7-pulsed autologous dendritic cells in patients with human papillomavirus type 16- and 18-positive cervical cancer. | human papillomavirus (hpv) type 16 (hpv 16) and hpv type 18 (hpv 18) are implicated in the induction and progression of the majority of cervical cancers. since the e6 and e7 oncoproteins of these viruses are expressed in these lesions, such proteins might be potential tumor-specific targets for immunotherapy. in this report, we demonstrate that recombinant, full-length e7-pulsed autologous dendritic cells (dc) can elicit a specific cd8(+) cytotoxic t-lymphocyte (ctl) response against autologous ... | 1999 | 10364287 |
| the human papillomavirus type 16 e6 gene alone is sufficient to induce carcinomas in transgenic animals. | high-risk human papillomaviruses (hpvs) are the causative agents of certain human cancers. hpv type 16 (hpv16) is the papillomavirus most frequently associated with cervical cancer in women. the e6 and e7 genes of hpv are expressed in cells derived from these cancers and can transform cells in tissue culture. animal experiments have demonstrated that e6 and e7 together cause tumors. we showed previously that e6 and e7 together or e7 alone could induce skin tumors in mice when these genes were ex ... | 1999 | 10364340 |
| detection of human papillomavirus types 6 and 11 in pubic and perianal hair from patients with genital warts. | genital human papillomavirus (hpv) types 6 and 11 are of clinical importance due to their role in the development of anogenital warts. a pilot study was performed to investigate whether dnas from hpv types 6 and 11 are present in hairs plucked from the pubic and perianal regions and eyebrows of patients with genital warts at present and patients with a recent history of genital warts. genital hpv dna was detected in 9 of 25 (36%) pubic hair samples and in 11 of 22 (50%) perianal hair samples by ... | 1999 | 10364596 |