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antinutrients content of some locally available legumes and cereals in nigeria.plant protein is the cheapest source of protein available to mankind but unfortunately the protein is accompanied by antinutrients. the quantity of oxalate and tannin in acha, bambara groundnut, guinea corn, millet, sesame seed, soybean and tiger nut were chemically analyzed. the white variety of sesame seed and soybean have the highest oxalate and tannin contents of 8.25 mg/g and 0.15 mg/g respectively. among the cereals the black and brown varieties of millet have the highest oxalate and tanni ...19921455533
carnivora: the primary structure of the major hemoglobin component from adult european lynx (lynx lynx, felidae).the complete primary structure of the major hemoglobin component from the adult european lynx (lynx lynx) is presented. presence of two hemoglobin components and three chains, beta a, beta b, and alpha, identified by gel electrophoresis. the purification of the globin chains achieved by ion-exchange chromatography. the globin chains were digested with trypsin. the peptide generated were purified by reversed-phase hplc. sequencing of the native chains up to 42 cycles and of the tryptic peptides w ...19921515033
the fate of dystrophin during the degeneration and regeneration of the soleus muscle of the rat.immunocytochemistry and western blotting were used to monitor the fate of dystrophin in the soleus muscle of the rat during a cycle of degeneration and regeneration induced by inoculation of the muscle with the venom of notechis scutatus scutatus (the australian tiger snake). in control muscle dystrophin was localised close to the plasma membrane. dystrophin began to break down 3-6 h after venom inoculation, giving a characteristic discontinuous labelling pattern. at 12 h dystrophin was absent f ...19921557946
a slowly inactivating k+ current in retinal ganglion cells from postnatal rat.the whole-cell configuration of the patch-clamp technique was used to study voltage-gated k+ conductances in retinal ganglion cells from postnatal rat. retinal ganglion cells were fluorescently labeled in situ, dissociated from the retina, and maintained in culture. with physiological solutions in the bath and the pipette, depolarizing voltage steps from physiological holding potentials activated na(+)-(ina), ca2+ (ica), and k(+)-currents studied previously in retinal ganglion cells. here we rep ...19921558829
species differences in amphibian olfactory neuron reactivity to a monoclonal antibody.a monoclonal antibody immunostained a subpopulation of olfactory sensory neurons in cryostat sections of the olfactory mucosa of the grass frog, rana pipiens, and the bullfrog, rana catesbeiana. however, in the olfactory tissues of the african clawed frog, xenopus laevis, only mucus and mucus-secreting components were stained, and no cell-specific immunoreactivity was seen in the tiger salamander, ambystoma tigrinum. this antibody is a useful marker of olfactory neuronal subpopulations in some a ...19921617462
axial motor organization in postmetamorphic tiger salamanders (ambystoma tigrinum): a segregation of epaxial and hypaxial motor pools is not necessarily associated with terrestrial locomotion.the axial motor column has undergone a major reorganization during the evolution of vertebrates. in aquatic anamniotes including lampreys, goldfish, and mudpuppies, epaxial and hypaxial motoneurons are intermingled in the column. in contrast, epaxial and hypaxial motoneurons are spatially segregated in water snakes, rats, and monkeys, apparently as a consequence of an isomorphic mapping of motoneuron location onto the position of innervated muscle in the embryonic myotome. the presence of these ...19921633553
light-dependent delay in the falling phase of the retinal rod photoresponse.using suction electrodes, photocurrent responses to 100-ms saturating flashes were recorded from isolated retinal rods of the larval-stage tiger salamander (ambystoma tigrinum). the delay period (tc) that preceded recovery of the dark current by a criterion amount (3 pa) was analyzed in relation to the flash intensity (if), and to the corresponding fractional bleach (r*0/rtot) of the visual pigment; r*0/rtot was compared with r*s/rtot, the fractional bleach at which the peak level of activated t ...19921739680
a double-label analysis demonstrating the non-coexistence of tyrosine hydroxylase-like and gaba-like immunoreactivities in amacrine cells of the larval tiger salamander retina.previous studies have localized tyrosine hydroxylase, the rate-limiting enzyme for the production of dopamine, and gamma-aminobutyric acid (gaba) to amacrine cell populations in the larval tiger salamander retina. double-label immunocytochemistry was used to examine if tyrosine hydroxylase-like and gaba-like immunoreactivities colocalize in tiger salamander amacrine cells. a total of 2,162 tyrosine hydroxylase-like immunoreactive amacrine cells were observed in double-labelled sections. none of ...19921363755
ionic selectivity of ih channels of rod photoreceptors in tiger salamanders.ionic selectivity of ih channels of tiger salamander rod photoreceptors was investigated using whole-cell voltage clamp. measured reversal potentials and the goldman-hodgkin-katz voltage equation were used to calculate permeability ratios with 20 mm k+ as a reference. in the absence of external k+, ih is small and hard to discern. hence, we defined ih as the current blocked by 2 mm external cs+. some small amines permeate ih channels, with the following permeability ratios (px/pk):nh4+, 0.17; me ...19921282144
hallervorden-spatz disease: clinical and mri study of 11 cases diagnosed in life.the diagnosis of hallervorden-spatz disease (hsd) has usually been made post mortem, although the recent description of characteristic abnormalities in the globus pallidus has suggested the possibility of an in vivo diagnosis. we present the clinical histories, neurological features and mri findings of 11 patients, diagnosed as having hsd. generalized dystonia with predominance of oromandibular involvement, behavioural changes followed by dementia and retinal degeneration were present in all the ...19921447570
tigers in jeopardy. 199217841157
contribution of ca and ca-activated cl channels to regenerative depolarization and membrane bistability of cone photoreceptors.1. cone photoreceptors in several vertebrate species generate ca-dependent regenerative depolarizations (e.g., ca spikes lasting up to 2 s) in response to current injection or surround illumination and may remain in a state of prolonged depolarization (e.g., a permanent plateau near 0 mv) after these stimuli. this paper, while confirming the role of ca channels in the regenerative depolarization, demonstrates that ca-activated cl channels either enhance or hinder prolonged depolarization, depend ...19921331354
calcium homeostasis in the outer segments of retinal rods from the tiger salamander.1. the processes regulating intracellular calcium in the outer segments of salamander rods have been investigated. the main preparation used was the isolated rod loaded with the ca(2+)-sensitive photoprotein aequorin, from which outer segment membrane current and free [ca2+]i could be recorded simultaneously. two other preparations were also used: outer segment membrane current was recorded from intact, isolated rods using a suction pipette, and from detached outer segments using a whole-cell pi ...19921282928
pharmacological properties of n-methyl-d-aspartate receptors on ganglion cells of an amphibian retina.1. the pharmacological characteristics of the n-methyl-d-aspartate (nmda) receptors on amphibian retinal ganglion cells were studied to determine their similarities or differences from nmda receptors found in mammalian central nervous system (cns) cells. cells were tested with a variety of nmda antagonists acting at sites on the nmda receptor/ion channel complex. 2. whole-cell voltage-clamp recordings were obtained from ganglion cells of the larval tiger salamander with a retinal slice preparati ...19921382121
the spatial relationship between müller cell processes and the photoreceptor output synapse.glutamate is the neurotransmitter released by photoreceptors in the retina. the postsynaptic action of glutamate is terminated partly by uptake into glial (müller) cells. the anatomical distribution of müller cell processes around the synaptic terminals of photoreceptors was investigated electron microscopically in the tiger salamander retina. müller cells wrap around the synaptic terminals of both rods and cones and come within 1-3 microns of the sites of glutamate release, close enough to cont ...19921325248
research, education, caries, and care--taming and turning the restorative tiger. 19921587968
cation interactions within the cyclic gmp-activated channel of retinal rods from the tiger salamander.1. the ionic dependence of current through the 3',5'-cyclic guanosine monophosphate (cyclic gmp)-activated channels of salamander rods was studied in excised inside-out membrane patches from isolated outer segments. voltage-clamp experiments on transducing rods were performed so that the channels in intact cells could be compared with those in excised patches. 2. the reversal potential of the cyclic gmp-induced patch current was close to the na+ equilibrium potential when the concentration of na ...19921381754
two types of glutamate receptors differentially excite amacrine cells in the tiger salamander retina.1. excitatory inputs to amacrine cells in the salamander retinal slice preparation were examined using whole-cell patch pipette voltage-clamp techniques. in strychnine (500 nm) and bicuculline (100 microm), two types of amacrine cell were easily distinguished by their light-evoked excitatory responses: transient and sustained. 2. in transient amacrine cells the current-voltage (i-v) relation for the peak light-evoked current was non-linear with a negative slope region between -50 and -70 mv. res ...19921355793
catecholamine effects on dissociated tiger salamander muller (glial) cells.dissociated muller (glial) cells from the neotenous tiger salamander retina respond electrogenically and rheogenically to three putative catecholamine (ca) neurotransmitters, epinephrine, norepinephrine and dopamine. all 3 cas stimulate a net inward current and an increase in input resistance (rn). the muller cell response to the cas is concentration dependent. at high concentrations ascorbate, an antioxidant used to protect the cas from oxidation, stimulated a net outward current and a decrease ...19921571780
a double-label study demonstrating that all serotonin-like immunoreactive amacrine cells in the larval tiger salamander retina express gaba-like immunoreactivity.a previous study localized serotonin-like immunoreactivity to amacrine cell populations in the larval tiger salamander retina. the present double-label immunocytochemical analysis of the tiger salamander retina was performed to determine if gamma-aminobutyric acid (gaba)-like immunoreactivity is expressed by serotonin-immunoreactive amacrine cells. more than 3,000 serotonin-amacrine cells were observed in double-label preparations, and all were found to express gaba-like immunoreactivity. this f ...19921504841
intracellular injection of vital dyes into single cells in the salamander olfactory epithelium.intracellular vital dye injection was used to examine the morphology of single sustentacular and receptor cells and the developmental fate of individual basal cells in the olfactory epithelium of the tiger salamander. in acute experiments, lucifer yellow injections were used to identify single basal, receptor or sustentacular cells on the basis of their overall morphology. dye-coupling between a number of the different epithelial cells was observed. progeny of basal cells were examined by follow ...19921380147
hypoprebetalipoproteinemia, acanthocytosis, retinitis pigmentosa, and pallidal degeneration (harp syndrome).we describe the clinical and laboratory studies of an 11-year-old girl with prominent orofacial dyskinesia, dystonia, and progressive dementia. investigations revealed hypoprebetalipoproteinemia, acanthocytosis, atypical retinitis pigmentosa, and evidence of iron deposition in the pallidal nuclei. electroneuromyography and skin and sural nerve biopsies were normal. the "eye-of-the-tiger" sign, used to describe the pallidal nuclei in hallervorden-spatz syndrome, was present on t2-weighted mris (g ...19921734303
neuronal ectopia in tiger retina.ocular pathology in a ten week-old tiger is described. in the retinae of both glaucomatous eyes, venous congestion and scattered hemorrhages were observed; rare perivascular infiltrates were seen. the basal lamina of the inner limiting membrane was considerably thickened, and serous exudates were widely distributed within the vitreous body. the retina was studied with a series of specific antibodies. müller (glial) cells were well developed and could be immuno-labelled by antibodies against both ...19921479197
the comparative myology of the thigh and crus in the salamanders ambystoma tigrinum and dicamptodon tenebrosus.variation in myology of the hind limb among salamanders has been poorly characterized. nineteen major hind limb muscles of ambystoma tigrinum (ambystomatidae) and dicamptodon tenebrosus (dicamptodontidae) were studied to provide baseline descriptive data on hind limb myology in salamanders and to generate hypotheses of hind limb muscle function. most superficial muscles of the hind limbs span multiple joints, including a unique three-joint muscle, the ischioflexorius, that extends from the pelvi ...199229865579
experimental morphology of the feeding mechanism in salamanders.the subarcualis rectus i muscle (sar) in the feeding mechanism of four tiger salamanders (ambystoma tigrinum) was removed early in ontogeny and these individuals were allowed to complete metamorphosis. this procedure resulted in postmetamorphic tiger salamanders which differed from control individuals in the size (and thus force generating capacity) of the sar muscle. the experimental manipulation of muscle ontogeny allowed a test of previous hypotheses of sar function in postmetamorphic individ ...199129865545
viral diseases of fish in japan.viruses causing infectious pancreatic necrosis (ipn) and infectious hematopoietic necrosis (ihn) were first isolated in japan during the 1970s and these two diseases remain among the most serious problems affecting cultured salmonids in japan. in addition to ihn and ipn, four other viral diseases cause major economic losses among cultured fishes in japan. these include viral pancreatic hepatic necrosis of yellowtail, rhabdovirus infection of japanese flounder and black rock fish, kuchishiro-sho ...199132288348
dissociation of lethal toxicity and enzymic activity of notexin from notechis scutatus scutatus (australian-tiger-snake) venom by modification of tyrosine residues.notexin from notechis scutatus scutatus snake venom was subjected to tyrosine modification with p-nitrobenzenesulphonyl fluoride (nbsf), and four modified derivatives were separated by h.p.l.c. the results of amino acid analysis and sequence determination revealed that only tyr-7, tyr-70 and tyr-77 were modified in notexin. modification of tyr-7 resulted in decreases in lethal toxicity and enzymic activity by 70.2% and 22.7% respectively. conversely, modification of tyr-77 caused a 1.8-fold incr ...19911764038
control of retinal information coding by gabab receptors.the directional selectivity of amacrine and ganglion cells was studied using conventional intracellular recording techniques and drug application in the superfused retina-eyecup preparation of the tiger salamander. baclofen, a gabab receptor agonist, enhanced normal directional responses in some directionally selective third-order neurons. in about 30% of the cells that were not normally directional, baclofen induced direction-selective responses. this effect was particularly marked when 2-amino ...19911646300
dual effect of glycine on horizontal cells of the tiger salamander retina.1. the effects of glycine on horizontal cells have been examined by microelectrode recording from superfused retinas isolated from the salamander. 2. low concentrations of glycine (less than 50 microm) hyperpolarized horizontal cells and increased the magnitude of their light responses. millimolar concentrations produced the opposite effect of depolarizing these cells and reducing their light response amplitudes. 3. in the presence of co2+ and mg2+ at concentrations sufficient to suppress the li ...19911812231
a double-label study demonstrating that enkephalin and somatostatin are localized in separate populations of amacrine cells in the larval tiger salamander retina.previous studies have localized enkephalin and somatostatin to amacrine cell populations in the larval tiger salamander retina. double-label immunocytochemistry was utilized to examine if enkephalin- and somatostatin-like immunoreactivities are colocalized to tiger salamander amacrine cells. of the more than 2000 labelled cells observed in double-labelled preparations, none were found to express both enkephalin and somatostatin immunoreactivity. therefore, these studies demonstrate that in the l ...19911686484
double-label analyses demonstrating the non-coexistence of enkephalin and glycine in amacrine cells of the larval tiger salamander retina.enkephalin immunocytochemistry was combined with either glycine immunocytochemistry or autoradiography of high-affinity glycine uptake to examine for colocalization of enkephalin and glycine in amacrine cells of the larval tiger salamander retina. a total of 995 enkephalin-immunoreactive amacrine cells were visualized in double-label preparations. none of the enkephalin-labelled cells was observed to co-label for markers of glycinergic activity.19911799867
amacrine cells in the tiger salamander retina: morphology, physiology, and neurotransmitter identification.amacrine cells of the vertebrate retina comprise multiple neurochemical types. yet details of their electrophysiological and morphology properties as they relate to neurotransmitter content are limited. this issue of relating light responsiveness, dendritic projection, and neurotransmitter content has been addressed in the retinal slice preparation of the tiger salamander. amacrine cells were whole-cell clamped and stained with lucifer yellow (ly), then processed to determine their immunoreactiv ...19911683878
cgmp-gated conductance in retinal bipolar cells is suppressed by the photoreceptor transmitter.transmitter release from photoreceptors is decreased by light, resulting in a conductance increase in depolarizing bipolar cells. addition of exogenous cgmp through a patch pipette to depolarizing bipolar cells from slices of dark-adapted tiger salamander retina resulted in an enhancement of the light response. this enhancement was blocked by gtp-gamma-s and dipyridamole, an inhibitor of phosphodiesterase. gtp-gamma-s and dipyridamole also blocked responses to exogenously applied 2-amino-4-phosp ...19911681833
coexistence and function of glutamate receptor subtypes in the horizontal cells of the tiger salamander retina.effects of the major glutamate receptor agonists, kainate (ka), alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid (ampa), quisqualate (qa), n-methyl-d-aspartate (nmda), l-alpha-amino-4-phosphonobutyrate (l-ap4), and trans-1-aminocyclopentane-1,3-dicarboxylic acid (acpd) on horizontal cells (hcs) were studied in superfused larval tiger salamander retina. 20 microm of ka, ampa, and qa mimicked the action of 3 mm glutamate in the absence and presence of 1 mm co2+. 20 microm 6-cyano-7-nitroqu ...19911661137
reciprocal latitudinal clines in oviposition behavior ofpapilio glaucus andp. canadensis across the great lakes hybrid zone: possible sex-linkage of oviposition preferences.the eastern tiger swallowtail butterfly,papilio glaucus, is the most polyphagous of all papilionidae species. while diverse larval detoxication abilities are known for bothpapilio glaucus and the closely relatedp. canadensis, the factors affecting oviposition preferences in adult females are unknown. these congeneric species were studied to determine the extent of oviposition mistakes on toxic plants. we were also interested in comparing the geographic patterns of variation in oviposition prefer ...199128313263
a cultural psychiatric study on tattoos of young korean males.in 2, 178 tattooed male conscripts in ages of 19-24 years, the most frequent tattoo was a heart mark or a mark of heart and arrow. the chinese characters which mean "one mind," eagles, dragons, some of 12 zodiacal animals, flowers, and flowers and butterflies or birds were also frequent. the ten korean symbols of long life and symbols which have been traditionally believed as having evil-repelling powers, for example, blue dragon or white tiger were also found. the frequency of images were diffe ...19911781185
fatal cerebral haemorrhage after tiger snake bite. 19911875858
fatal cerebral haemorrhage after tiger snake bite. 19912067450
a re-examination of enkephalin's coexistence with gamma-aminobutyric acid in amacrine cells of the larval tiger salamander retina.double-label immunocytochemistry was utilized to re-examine the colocalization of enkephalin and gamma-aminobutyric acid (gaba) in amacrine cells of the larval tiger salamander retina. a total of 465 enkephalin-immunoreactive amacrine cells were identified and in all cases these cells were gaba-immunoreactive. this finding corroborates a previous study that showed greater than 96% of enkephalin-amacrine cells in the tiger salamander retina to specifically accumulate [3h]gaba and provides additio ...19911913167
input-output relations of the feedback synapse between horizontal cells and cones in the tiger salamander retina.1. the input-output relation of the feedback synapse between horizontal cells (hcs) and cones was studied by simultaneously recording the light responses of the hcs and of cones the outer segments of which were truncated off. 2. the postsynaptic light response of the truncated cone was depolarizing and free of direct influence of photocurrents. these postsynaptic light responses were graded and sustained; their waveform resembled that of the hc light responses. 3. input-output relation of the hc ...19911651374
calling for bigger cats.i thought it would be helpful to put the record straight in response to g cornfield's letter on credit ratings for students on 'traditional' and project 2000 programmes (project 2000 paper tigers, nursing standard, april 3).199127662737
tiger teams draw researchers' snarls. 199117740923
project 2000 paper tigers.i am a second-year rmn student, and also the chair of the learners' council at my school of nursing.199127663341
synaptic connections, receptive fields, and patterns of activity in the tiger salamander retina. a simulation of patterns of activity formed at each cellular level from photoreceptors to ganglion cells [the friendenwald lecture]. 19912001922
depolarizing actions of gaba and glycine on amphibian retinal horizontal cells.1. the effects of inhibitory amino acid transmitters on horizontal cells in the superfused amphibian retina were studied by the use of conventional intracellular recording techniques. 2. gamma-aminobutyric acid (gaba) caused a calcium-independent depolarization of horizontal cells in mud puppy and tiger salamander. this action was mimicked by muscimol but not baclofen (bac) and blocked by bicuculline and picrotoxin (ptx), matching the gabaa receptor profile. 3. the purported gaba uptake inhibito ...19911646870
fatal cerebral haemorrhage after tiger snake (notechis scutatus) envenomation.this case report illustrates the threat to life posed by tiger snake venom-induced coagulopathy, the importance of first-aid, precautions with antivenom administration, the dose of antivenom and the necessity to monitor the coagulation status.19911953836
response properties of cones from the retina of the tiger salamander.1. spectral sensitivity measurements using the suction electrode technique reveal three types of cone in the retina of the tiger salamander, showing maximum sensitivity at wavelengths 610 nm (red-sensitive cone), 444 nm (blue-sensitive cone) and below 400 nm (uv-sensitive cone). 2. the absolute sensitivities of red- and blue-sensitive cones to flashes of optimal wavelength are 0.022 and 0.33 pa photon-1 micron 2 respectively. 3. the time-to-peak of the dim flash response and the recovery of memb ...19911841958
venom constituents of notechis scutatus scutatus (australian tiger snake) from differing geographic regions.column chromatography and polyacrylamide gel electrophoresis of notechis scutatus scutatus venom showed that the venoms from different geographical locations had variations in their constituents. the venom collected from south australia region contained both notexin and notechis ii-5. the relative quantity of notechis ii-5 was about three times that of notexin. on the other hand, the venom from victoria region contained large amounts of notexin, but lacked notechis ii-5. instead, an unknown nont ...19911814009
tiger assault: an unusual mode of head injury. 19911652402
isolation of a bacterium resembling pirellula species from primary tissue culture of the giant tiger prawn (penaeus monodon).during attempts to establish tissue cultures from hepatopancreas, heart, and hemolymph of the giant tiger prawn (penaeus monodon), using a medium including penicillin, streptomycin, and amphotericin b, bacterial contamination in the form of a sheet of growth attached to the tissue culture vessel was a persistent problem. contaminant bacteria were teardrop-shaped cells arranged in rosettes, and electron microscopy revealed buds, crateriform structures, and the absence of a peptidoglycan layer in ...19911781677
rescue and maturation in vitro of follicular oocytes collected from nondomestic felid species.the potential for rescuing immature oocytes from the ovaries of females of rare felid species which die or undergo medical ovariohysterectomy was evaluated. ovaries were recovered from 13 species representing 35 individuals in good-to-poor health. although the majority of females were 10 yr of age or older and in fair-to-poor health, a total of 846 oocytes were recovered of which 608 (71.9%) were classified as fair-to-excellent quality. one hundred of these oocytes were used for initial maturati ...19911805993
myosin isoform transitions and physiological properties of regenerated and re-innervated soleus muscles of the rat.soleus muscles in young female rats were destroyed by the local injection of the crude venom of the australian tiger snake, notechis scutatus and allowed to regenerate. the regenerated muscles consisted almost exclusively of type i muscle fibres, and histograms of fibre cross-sectional area were unimodal. in contrast the normal contralateral muscles consisted of a mixture of type i and iia fibres, and histograms of fibre cross-sectional area were bimodal. there was no change in the ability of th ...19911822353
present status of trichinellosis in japan.there have been three mass outbreaks of human trichinellosis in japan. the first was in 1974 in iwasaki, aomori prefecture, involving a group of hunters who ate raw meat of a black bear they had shot. of the 20 people who ate the bear meat raw, 15 showed clinical symptoms of trichinellosis. the second outbreak was experienced in sapporo, hokkaido, in 1980. the patients had eaten the raw meat of a brown bear served in a local restaurant. twelve people were diagnosed positive on the basis of clini ...19911822910
[scabies among the zoo mammals].scabies was observed in 9 species of mammals at the zoological garden of lódź in years 1957-1989. sarcoptes scabiei spp. was found in capybaras, tapirs and camelids. notoedres cati was recorded from the siberian tiger, but notoedres sp. from the erinaceus europaeus. scabies was also found in a wild dead tapla europaea at the zoo area.19911823472
[the relief of the luminal surface of the sinuses in the mammalian cranial dura mater].in mammalia with different types in organization of blood outflow in the dura mater venous sinuses: vertebral (tiger), jugular (fur-seal, cat, rabbit) and mixed (rat, dog, man) the internal surface relief of these sinuses has been studied. the total plan of the relief in all the species studied is principally the same. it is characterized with presence of visually determined macro-relief structures: pacchionian bodies, trabeculae, bars, eminences and excavations in places, where the sinuses fuse ...19911845512
effect of tissue inhomogeneity on dose distribution of continuous activity of low-energy electrons in bone marrow cavities with different topologies.monte carlo calculations have previously been performed by eckerman to evaluate the absorbed fractions of continuous sources of monoenergetic electrons in marrow cavities of human bone. the difference in scattering power of electrons in cortical bone (cb) and the red marrow (rm) was neglected. in the present work the integrated tiger series and electron-gamma-shower monte carlo codes were used to investigate the effect of topology of the bone and bone marrow interface on backscatter dose increas ...19911870497
the carotenoids of wild and blue disease affected farmed tiger shrimp (penaeus monodon, fabricus).1. the main carotenoids in wild penaeus monodon exoskeleton were astaxanthin di- and mono-esters, astaxanthin, and beta-carotene. 2. wild p. monodon exoskeleton contained on average 26.3 ppm total carotenoid; normally pigmented farmed shrimp had a similar concentration (25.3 ppm). 3. exoskeletons of farmed "blue" p. monodon (i.e. blue-coloured, as opposed to the normally red-blue/black banded shrimp) contained significantly less total carotenoid (4.3-7 ppm). the only major carotenoid being astax ...19911873990
immunologic conservation of the fiber cell beaded filament.lenses were obtained from the eyes of four different classes of chordates, including mammalia (rat, mouse, cow, human), aves (chicken), amphibia (tiger salamander), and osteichthyes (steelhead), as well as from one mollusca (squid). buffer soluble, urea soluble and urea insoluble fractions were prepared from each, and probed by western blot analysis for the presence of the lens fiber cell 115 and 49 kd beaded filament proteins. application of both polyclonal and monoclonal antibodies revealed th ...19911889232
diagnosis and prevalence of leptospira infection in aborted and stillborn horses.a study was conducted to evaluate a recently available fluorescent antibody test (fat) conjugate for the detection of leptospires in tissues of aborted and stillborn horses, to determine the leptospira antibody titers and compare serologic test results with fat results, and to determine the prevalence of leptospira-induced abortions and stillbirths in the equine population of central kentucky. from july 1, 1988 through june 30, 1989, 15 (2.5%) of 594 submissions (fetuses, stillborn foals, and/or ...19911892931
fatal and near-fatal animal bite injuries.fatal and near-fatal maulings of humans by pit bulls have recently become a topic of major public concern, resulting in the passage of laws in some jurisdictions that make the owner of a pit bull criminally liable for manslaughter if his or her pet causes a human death. the authors recently investigated two cases in which children were fatally injured by pet dogs. in the first case, a 17-day-old girl suffered fatal abdominal injuries when attacked by a pregnant siberian husky. a 2-year-old girl ...19911919485
cardiovascular effects of bpth-(1-34) and isoproterenol in the frog, rana tigrina.1. the cardiovascular effects of bovine parathyroid hormone fragment [bpth-(1-34)] and isoproterenol (iso) on frog (rana tigrina) isolated atria and helical strips of blood vessels were examined since pth produces a beta-adrenergic-like effect in the mammal. 2. data showed that both bpth-(1-34) and iso were vasorelaxant in kcl and arginine vasoctocin (avt) preconstricted dorsal aorta, iliac and femoral arteries. 3. they both relaxed extracellular calcium-dependent contrations. 4. there was no ad ...19911687555
effect of potassium ions and membrane potential on the na-ca-k exchanger in isolated intact bovine rod outer segments.two recent studies reported that na-ca exchange in the outer segments of tiger salamander rod photoreceptors (cervetto, l., lagnado, l., perry, r. j., robinson, d. w., and mcnaughton, p. a. (1989) nature 337, 740-743) and of bovine rod photoreceptors (schnetkamp, p. p. m., basu, d. k., and szerencsei, r. t. (1989) am. j. physiol. 257, c153-157) requires and transports k+ in a 4na/(1ca+1k) stoichiometry. in this study, we have examined the effects of k+ ions and membrane potential on the kinetics ...19911985892
a new host and locality record for mesocestoides sp. tetrathyridia (cestoidea: cyclophyllidea), with a summary of the genus from snakes of the world.a new host and distribution record is reported for tetrathyridia of mesocestoides sp. one of 5 (20%) namib tiger snakes, telescopus beetzi, from south africa was infected. numerous tetrathyridia were found encapsulated in mesentery attached to the small intestine. morphological examination of tetrathyridia revealed absence of buds, multiple scoleces, or any other evidence of asexual proliferation. a summary of the snakes of the world reported as hosts of tetrathyridia of mesocestoides sp. is pre ...19912010871
a test of a disease-avoidance model of animal phobias.this study examined the relationship between disgust/contamination sensitivity and fear of animals. the results suggested that sensitivity to disgust and contamination was directly related to scores on the animal phobia and fear of illness and death sub-scales of the fear survey schedule (fss). further analysis suggested that disgust/contamination sensitivity was related only to fear of certain groups of animals: namely those animals that are not considered to attack and harm human beings but ar ...19912012593
renal papillary necrosis in two domestic cats and a tiger. 19912017832
new toxins from the venom of the common tiger snake (notechis scutatus scutatus).scutoxin a and b represent two isoforms of a new toxic protein from the venom of the australian tiger snake, notechis scutatus scutatus. both isoforms, of apparent mol. wt 13,000, are less basic than either notexin or notechis ii-5. they both have similar i.v. ld50-values in mice of ca 0.006 micrograms/g, and phospholipase activities of about 136 mumoles of fatty acid released/min/mg at 37 degrees c when acting on phosphatidylcholine in the presence of triton x-100. toxicities of the scutoxins a ...19912028475
immunological relationships between the subunits of textilotoxin and rabbit antisera raised against textilotoxin and some snake venoms.the binding of textilotoxin and its subunits a, b, c and d to polyclonal rabbit antisera directed against textilotoxin, australian common brown snake (pseudonaja textilis) and tiger snake (notechis scutatus scutatus) venoms was studied by elisa. subunit d showed greatest reactivity with antisera to textilotoxin and brown snake venom. the phospholipase a2 active subunit a reacted more strongly with antisera to tiger snake venom in keeping with the high degree of homology between the amino acid se ...19912048151
crustacean hyperglycemic hormone induced alterations in millepede (spirostreptus asthenes) carbohydrate metabolism.following intersegmental injection of the hyperglycemic hormone (hgh) obtained from fresh water crab (oziotelphusa senex senex) and marine tiger prawn (penaeus monodon), hemolymph sugar concentration was significantly increased in millepede (spirostreptus asthenes). glycogen and total carbohydrates of fat body decreased in injected animals indicating that the source of hyperglycemia was carbohydrates from the fat body. correlating with the decreased glycogen content, phosphorylase activity of th ...19911725740
reduced calcium and inhibition of protein kinase c mimic the enhancement of ornithine decarboxylase activity of prolactin in ambystoma tigrinum tissues.we previously reported that prolactin (prl) could increase the activity of ornithine decarboxylase (odc) in liver slices taken from larval tiger salamanders (ambystoma tigrinum). this action of the hormone was inhibited by oxytocin (ot), the calcium ionophore a23187, and diacyglycerol (dg) and was duplicated by 10 microm verapamil (vml), a calcium channel blocker. here, we expand these results to show that 1) a higher dose of vml (50 microm) produces an additive effect with prl; 2) addition of s ...19911940822
pathogens as a factor limiting the spread of cannibalism in tiger salamanders.intraspecific predation is taxonomically widespread, but few species routinely prey on conspecifics. this is surprising as conspecifics could be a valuable resource for animals limited by food. a potential cost of cannibalism that has been largely unexplored is that it may enhance the risk of acquiring debilitating pathogens or toxins from conspecifics. we examined how pathogens affect variation in the incidence of cannibalism in tiger salamander larvae (ambystoma tigrinum nebulosum), which occu ...199128312127
collagen type conservation during metamorphic repatterning of the dermal fibers in salamanders.the orientation of the fibers in the dermis of the tiger salamander, ambystoma tigrinum, undergoes a dramatic repatterning at metamorphosis. the pre-metamorphic, larval dermis is a tight layer composed of crossed fibers that wind helically around the trunk. this condition is retained by neotenic adults which do not undergo metamorphosis. in contrast, the neotenic adults which do not undergo metamorphosis. in contrast, the metamorphosed adult dermis consists of a superficial, loose network of fib ...19911890686
blockage and permeation of divalent cations through the cyclic gmp-activated channel from tiger salamander retinal rods.1. blockage and permeation of divalent cations through channels activated by guanosine 3',5'-cyclic monophosphate (cyclic gmp) were studied in membrane patches excised from retinal rods of the tiger salamander ambystoma tigrinum by rapidly changing the ionic medium bathing the intracellular side of the excised membrane. 2. the na+ current, observed when 110 mm-nacl was present on both sides of the membrane patch, was reduced by the addition of 1 mm of the chloride salts of ca2+, mg2+, sr2+, ba2+ ...19911725182
pennsylvania's hcccc (health care cost containment council): cost/quality watchdog or paper tiger? 199110115135
multiple prismatic calcium phosphate layers in the jaws of present-day sharks (chondrichthyes; selachii).jaws of large individuals, over 2 m in total length, of the shark species carcharodon carcharias (great white shark) and isurus oxyrinchus (mako shark) of the family lamnidae, and galeocerdo cuvieri (tiger shark) and carcharhinus leucas (bull shark) of the family carcharhinidae were found to have multiple, up to five, layers of prismatic calcium phosphate surrounding the cartilages. smaller individuals of these species and other known species of living chondrichthyans have only one layer of pris ...19911999241
similar properties of cgmp-activated channels between cones and rods in the carp retina.using patch-clamp techniques, properties of cgmp-activated channel were studied at a single-channel level in order to examine (1) whether any differences are recognized between the cgmp-activated channels of rods and cones in the same animal species, and (2) whether the channel properties of the same photoreceptor class differ in different animal species. experiments were performed on inside-out membrane patches excised from outer segments of rods and morphological subtypes of cones in the carp ...19911715743
arctiid moth clicks can degrade the accuracy of range difference discrimination in echolocating big brown bats, eptesicus fuscus.four big brown bats (eptesicus fuscus) born and raised in captivity were trained using the yes/no psychophysical method to report whether a virtual sonar target was at a standard distance or not. at threshold bats were able to detect a minimum range difference of 6 mm (a delta t of 36 microseconds). following threshold determinations, a click burst 1.8 ms long containing 5 pulses from the ruby tiger moth, phragmatobia fuliginosa (arctiidae), was presented randomly after each phantom echo. the so ...19911920158
variation in venom composition and reactivity in two specimens of yellow-faced whip snake (demansia psammophis) from the same geographical area.gel filtration chromatography and sds-page of venom from two specimens of demansia psammophis showed little similarity. amidolytic activity of the venoms, however, was in the same order of reactivity against various chromogenic substrates. the venom from both snakes produced precipitin lines with brown snake antivenom but the venom detection kit (commonwealth serum laboratories) identified one venom as brown snake (pseudonaja sp.) and the other as tiger snake (notechis sp.). these results raise ...19902128423
voltage-dependent ionic currents in taste receptor cells of the larval tiger salamander.voltage-dependent membrane currents of cells dissociated from tongues of larval tiger salamanders (ambystoma tigrinum) were studied using whole-cell and single-channel patch-clamp techniques. nongustatory epithelial cells displayed only passive membrane properties. cells dissociated from taste buds, presumed to be gustatory receptor cells, generated both inward and outward currents in response to depolarizing voltage steps from a holding potential of -60 or -80 mv. almost all taste cells display ...19901701829
spatial organization of the bipolar cell's receptive field in the retina of the tiger salamander.1. the spatial properties of rods, horizontal cells and bipolar cells were studied by intracellular recording in the isolated, perfused retina of the tiger salamander, ambystoma tigrinum. low stimulus intensities were used in order to keep cell responses close to, or within, their linear intensity/response range. 2. spatial properties of bipolar cell receptive fields, measured while perfusing with normal ringer solution, were compared with those measured during exposure to agents that eliminated ...19902348392
transduction noise induced by 4-hydroxy retinals in rod photoreceptors.new visual pigments were formed with 4-hydroxy retinals in isolated vertebrate rod photoreceptors by exposing bleached rods from the tiger salamander, ambystoma tigrinum, to lipid vesicles containing the analogues. formation of physiologically active pigment was demonstrated by the restoration of sensitivity and by a shift of approximately 50 nm in the peak of both the visual pigment absorptance spectrum and rod spectral sensitivity spectrum from approximately 520 to approximately 470 nm for 11- ...19902297557
partial amino acid sequence of erythrocyte carbonic anhydrase from tiger shark.1. a partial primary structure (197 residues) of carbonic anhydrase from tiger shark (galeocerdo cuvieri) erythrocytes has been determined. 2. the amino acid sequence of the enzyme is identical to those of human cytoplasmic carbonic anhydrases (ca i-iii) by as much as 52-60%. 3. it is shown that tiger shark ca most closely resembles the ca ii isoenzyme of amniotes. 4. isoelectric focusing and inhibition studies on carbonic anhydrase from dogfish (squalus acanthias) blood and muscle indicate the ...19902110050
the presence of a thyrotropin-releasing hormone-like factor in the grass shrimp (black tiger prawn, penaeus monodon)immunoreactive (trh) has been detected in acid and methanol extracts of hepatopancreas, in grass shrimp (black tiger prawn, penaeus monodon). the evidence provided by the stimulation of thyrotropin (tsh) release from perfused rat anterior pituitary glands in vitro following the application of hepatopancreas extracts (she) reflected the presence of trh-like substance in the shrimp. however, the molecular weight of the trh-like substance estimated by gel filtration in she was greater than that of ...19902124182
on the purification of notexin. isolation of a single amino acid variant from the venom of notechis scutatus scutatus.venom of the australian tiger snake, notechis scutatus scutatus was fractionated by conventional ion-exchange chromatography. the fraction containing notexin, a well-known single-chain toxic phospholipase a2, was further purified by reverse-phase high-performance liquid chromatography. two main components were isolated and the major one corresponded to notexin. the other component, designated as notechis ns, was an isoform of notexin. notechis ns and notexin possessed similar in vitro esterase a ...19902155818
numerical analysis of electrophoretic protein patterns of group ef-4 bacteria, predominantly from dog-bite wounds of humans.thirty-seven strains of group ef-4 bacteria (from various countries) were characterized by one-dimensional sds-page of cellular proteins. they comprised 21 from dog-bite wounds of humans, three from cat-bite wounds of humans and five from human limb wounds which may have been inflicted by dogs or cats; there was also one each from a pet monkey, a tiger lung (fatal), a dog tonsil, a mouse, a cat liver, a wallaby mandible, a human vagina and one from a human limb wound which was apparently not inf ...19902179200
an immunohistochemical study of endocrine cells in the proximal duodenum of eight marsupial species.the proximal duodenum of eight marsupial species, (koala, common brushtail possum, ring-tailed possum, common wombat, great grey kangaroo, parma wallaby, short-nosed bandicoot and tiger cat) were investigated immunohistochemically using 12 specific antisera for gut hormones. several types of immunoreactive cells were seen on the intestinal villi and in crypts of these species: 9 types in the koala; 8 types in the common brushtail possum; 7 types in the common wombat; 6 types in the short-nosed b ...19902182587
molecular genetic divergence of orang utan (pongo pygmaeus) subspecies based on isozyme and two-dimensional gel electrophoresis.the orang utan (pongo pygmaeus), as currently recognized, includes two geographically separated subspecies: pongo pygmaeus pygmaeus, which resides on borneo, and p. p. abelii, which inhabits sumatra. at present, there is no known route of gene flow between the two populations except through captive individuals which have been released back into the wild over the last several decades. the two subspecies are differentiated by morphological and behavioral characters, and they can be distinguished b ...19902230076
existence of a gonadotropin-releasing hormone-like factor in the grass shrimp (black tiger prawn, penaeus monodon)the immunological and biological gonadotropin-releasing hormone (gnrh)-like activities in the acid extract of shrimp hepatopancreas (she) were studied. the immunological activity determined by a gnrh radioimmunoassay was 22.3 +/- 2.7 ng per ml of she. she increased luteinizing hormone (lh) release from rat anterior pituitary glands (aps) in vitro, and its biological activity was 42.9 +/- 28.5 ng per ml. no immunological activity was found in the acid extracts of eye ball, eye stalk, nerve and he ...19902257769
[the role of magnetic resonance imaging in the diagnosis of hallervorden-spatz disease].a mentally-retarded 21-year-old female with narrowing of the visual field was admitted to our hospital because of dystonia in the body and extremities which appeared 6 months before and was gradually exacerbating. on admission, torsion dystonia, pyramidal tract sign, and retinitis pigmentosa with optic atrophy, urinary incontinence, hyperhidrosis and insomnia were noted. on the wechsler adult intelligence scale, she achieved less than 60 for verbal-iq. laboratory tests including serum copper and ...19902265508
in vitro fertilization and embryo development in vitro and in vivo in the tiger (panthera tigris).a study was conducted to evaluate the adaptability to the tiger of an in vitro fertilization/embryo culture system previously developed in the domestic cat. in trial i (july 1989), 10 female tigers were treated with either 2,500 (n = 5) or 5,000 (n = 5) iu ecg i.m. and with 2,000 iu hcg i.m. 84 h later. in trial ii (january 1990), 6 females (5 of which were treated in trial i) were given 2,500 iu ecg i.m. and 2,000 iu hcg i.m. 84 h later. twenty-four to twenty-six hours after hcg treatment, all ...19902291909
comparison of serum and plasma taurine values in bengal tigers with values in taurine-sufficient and -deficient domestic cats.a white bengal tiger was determined to have a central retinal lesion and a central visual defect. because of the known association between feline central retinal degeneration (crd) and taurine deficiency in domestic cats, plasma concentrations of taurine were measured in this tiger. serum concentrations of taurine, methionine, and cystine also were measured in white bengal tigers, orange bengal tigers, taurine-sufficient domestic cats, and taurine-deprived and tissue-taurine-depleted visually im ...19902298663
the lateral spread of signal between bipolar cells of the tiger salamander retina.when mapped with a small spot of light, the central receptive fields of bipolar cells in the salamander retina are much larger than the extent of bipolar cell dendrites. furthermore responses of bipolar cells to distant spots of light are considerably delayed relative to proximal spots. using quantitative modelling, electrical coupling between bipolar cells is examined and rejected as a sufficient explanation of the data. an active process appears to shape signal waveform as signals spread later ...19902357478
the assessment of muscle fibre loss after the injection of the venom of notechis scutatus (australian tiger snake).we have used the w.h.o. international reference venom from the australian tiger snake, notechis scutatus, to study possible methods for the assessment of local myonecrosis caused by this venom. we made subcutaneous injections of various doses (0.25-20.0 micrograms) of venom into the antero-lateral aspect of the rat hind limb. the soleus muscle was removed after 24 hr and muscle fibre loss calculated from photo-montages of histological sections. muscle tissue which had been either frozen or wax-e ...19901692644
evidence for a fel d i-like molecule in the "big cats" (felidae species).in this study, we investigated the cross-reactivity pattern of ige and igg4 antibodies to the major feline allergen, fel d i. we studied the ige and igg4 response of 11 cat-allergic patients against fel d i-like structures in eight members of the felidae family: ocelot, puma, serval, siberian tiger, lion, jaguar, snow leopard, and caracal. hair from these "big cats" was collected, extracted, and used in a rast system and histamine-release test. by means of a rast-inhibition assay with affinity-p ...19901695231
[capillary length in the human heart during physiological growth and under pathological conditions].knowledge about the capillary length in mammalian and human heart is still scanty. because it is very difficult to examine this parameter directly we used an indirect method. the distance between the arterioles can be used as an approximately measurement for the double capillary length. we tried to measure the distances between arterioles in about 30 postmortem injected hearts using india ink to produce a kind of reflected imitated tabby (tiger) heart pattern. in addition we measured the distanc ...19901708599
the cardiac effects of parathyroid hormone in the tiger frog, rana tigrina.1. the cardiac effects of the n-terminal (1-34) peptide fragment of bovine parathyroid hormone [bpth-(1-34)] on isolated atria were examined in the frog, rana tigrina. 2. bpth-(1-34) produced dose-related inotropic response but no chronotropic response. this inotropic response varied at different times of the year. 3. the inotropic effect of bpth-(1-34) was attenuated in the presence of verapamil and imidazole. 4. the mechanism of action of bpth-(1-34) is probably a stimulation of calcium influx ...19901977549
synaptic transmission to the horizontal cells in the retina of the larval tiger salamander.1. the receptive field diameter for most horizontal cells far exceeds the lateral spread of processes for any cell. therefore horizontal cells probably receive synaptic input from neighbours as well as from the photoreceptors. the electrical effects of these two synaptic inputs were studied. 2. we have characterized the electrical properties of the horizontal cell inputs by determining the current-voltage curves in dark and light. these curves were compared with those obtained in the presence of ...1990209177
population structure and host use in hybridizing subspecies of papilio glaucus (lepidoptera: papilionidae).two parapatric subspecies of the eastern tiger swallowtail butterfly, papilio glaucus glaucus and p. g. canadensis, differ greatly in larval survival and growth on host plants in the magnoliaceae, salicaceae, and betulaceae. in the first part of this study, butterflies were collected from 17 sites along a transect from georgia to quebec and used for allozyme electrophoresis. two x- (or z-)linked enzyme loci (ldh and pgd) showed allele frequency differences that delineated a hybrid zone between t ...199028564427
the effects of quinidine on sodium-dependent calcium efflux in isolated rod photoreceptors of the salamander retina.the effect of quinidine on the membrane current generated by the na:ca, k exchange has been investigated in the outer segment of isolated rod photoreceptors from the retina of the larval tiger salamander. the inward exchange current associated with the efflux of ca2+ was selectively recorded by introducing a ca2+ load through the light-sensitive channels, and then shutting these channels with a bright light. quinidine (20-1000 microm) reduced the magnitude of the exchange current and slowed its ...19901707516
sensitization of bleached rod photoreceptors by 11-cis-locked analogues of retinal.photoactivation of rhodopsin initiates both excitation and adaptation in vertebrate rod photoreceptors. bleaching of rhodopsin to free opsin and all-trans-retinal in isolated rods produces a stable desensitization (bleaching adaptation) that is much larger than expected from pigment depletion alone. in our experiments, a 93% bleach produced a 500-fold increase in the light intensity required for saturation of the light response. this component of adaptation was 32-fold larger than the 16-fold in ...19902395874
effect of season on seminal traits and serum hormone concentrations in captive male siberian tigers (panthera tigris).electroejaculates and serum samples were collected throughout the year from 5 male siberian tigers. semen quality, seminal plasma chemistry and serum hormone concentrations were evaluated and analysed as a function of season. semen volume, concentration, motility, viability and morphology were not influenced by season. the ability of spermatozoa to undergo capacitation and penetrate zona-free hamster eggs did not differ due to season. season had no influence on seminal plasma concentrations of a ...19902231534
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