Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| chitosan, carbon quantum dot, and silica nanoparticle mediated dsrna delivery for gene silencing in aedes aegypti: a comparative analysis. | in spite of devastating impact of mosquito borne pathogens on humans, widespread resistance to chemical insecticides and environmental concerns from residual toxicity limit mosquito control strategies. we tested three nanoparticles, chitosan, carbon quantum dot (cqd), and silica complexed with dsrna, to target two mosquito genes (snf7 and src) for controlling aedes aegypti larvae. relative mrna levels were quantified using qrt-pcr to evaluate knockdown efficiency in nanoparticle-dsrna treated la ... | 2015 | 26291176 |
| angelica sinensis (umbelliferae) with proven repellent properties against aedes aegypti, the primary dengue fever vector in thailand. | botanical resources with great diversity in medicinal and aromatic plants are a rich and reliable source for finding insect repellents of plant origin, which are widely popular among today's consumers. although some herbal-based repellents have been proven comparable to or even better than synthetics, commercially available natural repellents generally tend to be expensive, with short-lived effectiveness. this critical flaw leads to ongoing research for new and effective repellents, which provid ... | 2015 | 25773182 |
| aedes aegypti salivary gland extract ameliorates experimental inflammatory bowel disease. | current therapies for inflammatory bowel disease (ibd) are not totally effective, resulting in persistent and recurrent disease for many patients. mosquito saliva contains immunomodulatory molecules and therein could represent a novel therapy for ibd. here, we demonstrated the therapeutic activity of salivary gland extract (sge) of aedes aegypti on dextran sulfate sodium (dss)-induced colitis. for this purpose, c57bl/6 male mice were exposed to 3% dss in drinking water and treated with sge at ea ... | 2015 | 25770821 |
| evaluation of some aromatic plant extracts for mosquito larvicidal potential against culex quinquefasciatus, aedes aegypti, and anopheles stephensi. | in the present investigation, larvicidal potential of hexane, choloroform, ethyl acetate, acetone, and methanol extracts of seven aromatic plants, viz., blumea mollis, chloroxylon swietenia, clausena anisata, feronia limnonia, lantana camera, plectranthus amboinicus, and tagetes erecta were screened against culex quinquefasciatus, aedes aegypti, and anopheles stephensi. the larval mortality was observed after 12 and 24 h of exposure period. the results revealed that all the extracts showed varie ... | 2015 | 25630696 |
| dengue in brazil and colombia: a study of knowledge, attitudes, and practices. | introduction this study was conducted in brazil and colombia,where dengue is endemic and vector control programs use chemical insecticides. methods we identified knowledge, attitudes, and practices about dengue and determined the infestation levels of aedes aegypti in one brazilian and four colombian communities. results the surveys show knowledge of the vector, but little knowledge about diagnosis, prognosis, and treatment. vector infestation indices show brazil to have good relative control, w ... | 2015 | 25626660 |
| immature aedes mosquitoes colonize culex quinquefasciatus breeding sites in neighborhoods in the municipality of olinda, state of pernambuco. | the present study shows the colonization of aedes mosquitoes in breeding sites specific for culex quinquefasciatus in neighborhoods in the municipality of olinda. | 2015 | 25626658 |
| cytostatic and genotoxic effect of temephos in human lymphocytes and hepg2 cells. | temephos is an organophosphorus pesticide that is used in control campaigns against aedes aegypti mosquitoes, which transmit dengue. in spite of the widespread use of temephos, few studies have examined its genotoxic potential. the aim of this study was to evaluate the cytotoxic, cytostatic and genotoxic effects of temephos in human lymphocytes and hepatoma cells (hepg2). the cytotoxicity was evaluated with simultaneous staining (fda/etbr). the cytostatic and genotoxic effects were evaluated usi ... | 2015 | 25746384 |
| rnai knockdown of bmrab3 led to larva and pupa lethality in silkworm bombyx mori l. | rab3 gtpases are known to play key a role in vesicular trafficking, and express highest in brain and endocrine tissues. in mammals, rab3 gtpases are paralogs unlike in insect. in this study, we cloned rab3 from the silk gland tissue of silkworm bombyx mori, and identified it as bmrab3. our in silico analysis indicated that bmrab3 is an isoform with a theoretical isoelectric point and molecular weight of 5.52 and 24.3 kda, respectively. further, bmrab3 showed the c-terminal hypervariability for g ... | 2015 | 25735242 |
| effect of temperature on life history traits during immature development of aedes aegypti and culex quinquefasciatus (diptera: culicidae) from córdoba city, argentina. | we investigated how ambient temperature under fluctuating conditions affects the larval-pupal immature traits of aedes aegypti and culex quinquefasciatus mosquitoes from córdoba city, argentina, and established each species development threshold and physiological time. based on life tables, three cohorts of each mosquito species were reared in the laboratory under small fluctuating temperatures conditions of 15.2±1.7°c, 17.9±1.6°c, 21.6±0.7°c and 25.3±0.4°c for ae. aegypti, and 16.6±1.7°c, 18.7± ... | 2015 | 25733491 |
| identification of chemical constituents and larvicidal activity of essential oil from murraya exotica l. (rutaceae) against aedes aegypti, anopheles stephensi and culex quinquefasciatus (diptera: culicidae). | this study was conducted to evaluate the phytochemical composition and larvicidal effect of leaf essential oil from murraya exotica against early fourth-instar larvae of aedes aegypti, anopheles stephensi and culex quinquefasciatus. gas chromatography (gc) and gas chromatography mass spectrometry (gc-ms) analyses revealed that the essential oil contained 27 components. the major chemical components identified were β-humulene (40.62%), benzyl benzoate (23.96%), β-caryophyllene (7.05%) and α-terpi ... | 2015 | 25697880 |
| tackling the growing threat of dengue: phyllanthus niruri-mediated synthesis of silver nanoparticles and their mosquitocidal properties against the dengue vector aedes aegypti (diptera: culicidae). | mosquitoes are vectors of devastating pathogens and parasites, causing millions of deaths every year. dengue is a mosquito-borne viral infection found in tropical and subtropical regions around the world. recently, transmission has strongly increased in urban and semiurban areas, becoming a major international public health concern. aedes aegypti (diptera: culicidae) is the primary vector of dengue. the use of synthetic insecticides to control aedes mosquitoes lead to high operational costs and ... | 2015 | 25669140 |
| synthesis and characterization of silver nanoparticles using gmelina asiatica leaf extract against filariasis, dengue, and malaria vector mosquitoes. | mosquitoes are blood-feeding insects and serve as the most important vectors for spreading human diseases such as malaria, yellow fever, dengue fever, and filariasis. the continued use of synthetic insecticides has resulted in resistance in mosquitoes. synthetic insecticides are toxic and affect the environment by contaminating soil, water, and air, and then natural products may be an alternative to synthetic insecticides because they are effective, biodegradable, eco-friendly, and safe to envir ... | 2015 | 25666372 |
| mukia maderaspatana (cucurbitaceae) extract-mediated synthesis of silver nanoparticles to control culex quinquefasciatus and aedes aegypti (diptera: culicidae). | mosquitoes and mosquito-borne diseases are prone to raise health and economic impacts. synthetic insecticide-based interventions are indeed in situations of epidemic outbreak and sudden increases of adult mosquitoes. nanoparticles are being used in many commercial applications and were found that aqueous silver ions can be reduced by an aqueous extract of plant parts to generate extremely stable silver nanoparticles in water. based on this, silver nanoparticles (snps) were synthesized using leaf ... | 2015 | 25601441 |
| insecticidal and repellent activity of clausena dentata (rutaceae) plant extracts against aedes aegypti and culex quinquefasciatus mosquitoes (diptera: culicidae). | mosquito control is facing a threat due to the emergence of resistance to synthetic insecticides. insecticides of botanical origin may serve as suitable alternative biocontrol agents. the present study is to evaluate adulticidal activity of clausena dentata plant extract against aedes aegypti and culex quinquefasciatus mosquitoes. the adult mortality was observed after 24 h of exposure. the highest mortality was found in acetone extracts against ae. aegypti and cx. quinquefasciatus with the lc50 ... | 2015 | 25573693 |
| hptlc analysis of scoparia dulcis linn (scrophulariaceae) and its larvicidal potential against dengue vector aedes aegypti. | this study evaluates the larvicidal activity of scoparia dulcis aqueous extract against dengue vector and determines its major chemical components. the extract was tested at various concentrations ranging from 0.1 to 2 mg/ml against aedes aegypti larvae. the extracts displayed significant larvicidal efficacy against ae. aegypt species after 24 h exposure revealing lc50 of 3.3835 (mg/ml) and lc90 of 5.7578 (mg/ml). finger printing profile carried out by camag automatic tlc sample applicator progr ... | 2015 | 25573588 |
| aealrack1 expression and localization in response to stress in c6/36 ht mosquito cells. | the receptor for activated c kinase 1 (rack1), a scaffold protein member of the tryptophan-aspartate (wd) repeat family, folds in a seven-bladed β-propeller structure that permits the association of proteins to form active complexes. mosquitoes of the genus aedes sp., are vectors of virus producing important diseases such as: dengue, chikungunya and yellow fever. based on the highly conserved gene sequence of aeaerack1 of the mosquito aedes aegypti we characterized the mrna and protein of the ho ... | 2015 | 25555378 |
| mosquito larvicidal potential of silver nanoparticles synthesized using chomelia asiatica (rubiaceae) against anopheles stephensi, aedes aegypti, and culex quinquefasciatus (diptera: culicidae). | mosquitoes transmit serious human diseases, causing millions of deaths every year. mosquito control is to enhance the health and quality of life of county residents and visitors through the reduction of mosquito populations. mosquito control is a serious concern in developing countries like india due to the lack of general awareness, development of resistance, and socioeconomic reasons. today, nanotechnology is a promising research domain which has a wide ranging application in vector control pr ... | 2015 | 25544703 |
| chemical composition and biological activity of four salvia essential oils and individual compounds against two species of mosquitoes. | the chemical compositions of essential oils obtained from four species of genus salvia were analyzed by gas chromatography with a flame ionization detector (gc-fid) and gas chromatography-mass spectrometry (gc-ms). the main compounds identified from salvia species essential oils were as follows: 1,8-cineole (71.7%), α-pinene (5.1%), camphor (4.4%), and β-pinene (3.8%) in salvia apiana; borneol (17.4%), β-eudesmol (10.4%), bornyl acetate (5%), and guaiol (4.8%) in salvia elegans; bornyl acetate ( ... | 2015 | 25531412 |
| effect of confertifolin from polygonum hydropiper l. against dengue vector mosquitoes aedes aegypti l. | the essential oil from the leaves of polygonum hydropiper l. (polygonaceae) was tested against aedes aegypti l. the lc50 values were 190.72 and 234.37 ppm against second and fourth instar larvae of a. aegypti, respectively. confertifolin (6,6,9a-trimethy l-4,5,5a,6,7,8,9,9a-octahydronaphtho [1,2-c] furan-3 (1h)-one) was isolated from the essential oil of p. hydropiper leaves using silica gel column chromatography. the lc50 values were 2.90 and 2.96 ppm for second and fourth instar larvae of a. a ... | 2015 | 25523289 |
| são paulo urban heat islands have a higher incidence of dengue than other urban areas. | urban heat islands are characterized by high land surface temperature, low humidity, and poor vegetation, and considered to favor the transmission of the mosquito-borne dengue fever that is transmitted by the aedes aegypti mosquito. we analyzed the recorded dengue incidence in sao paulo city, brazil, in 2010-2011, in terms of multiple environmental and socioeconomic variables. geographical information systems, thermal remote sensing images, and census data were used to classify city areas accord ... | 2015 | 25523076 |
| bioactive compound synthesis of ag nanoparticles from leaves of melia azedarach and its control for mosquito larvae. | larvicidal activity of synthesized ag nanoparticles using 2,7.bis[2-[diethylamino]-ethoxy]fluorence isolate from the melia azedarach leaves against aedes aegypti and culex quinquefasciatus. six fractions were collected and concentrated, fraction three showed a single spot on tlc which was found to be a pure compound. the structures were elucidated by analyses of uv, ms, and nmr spectral data. the maximum mortality was fluorence against a. aegypti and c. quinquefasciatus (lc50 = 7.94, lc90 = 23.8 ... | 2015 | 25496834 |
| physiological and biochemical effects of botanical extract from piper nigrum linn (piperaceae) against the dengue vector aedes aegypti liston (diptera: culicidae). | the leaves of piper nigrum l. (piperaceae) were evaluated for chemical constituents and mosquito larvicidal activity against the larvae of aedes aegypti. gc and gc-ms analyses revealed that the crude extracts contain 16 compounds. thymol (20.77%) and ç-elemene (10.42%) were identified as the major constituents followed by cyclohexene, 4-ethenyl-4-methyl-3-(1-methylethenyl)-1-(1 methylethyl)-, (3r-trans) (7.58%), 4,6-octadienoic acid, 2-acetyl-2-methyl-, ethyl ester (6.98), 2(3h)-furanone, 3,4-bi ... | 2015 | 26277727 |
| green synthesis of silver nanoparticles from cassia roxburghii-a most potent power for mosquito control. | mosquitoes transmit serious human diseases, causing millions of deaths every year. the use of synthetic insecticides to control vector mosquitoes has caused physiological resistance and adverse environmental effects in addition to high operational cost. insecticides of synthesized natural products for vector control have been a priority in this area. in the present study, silver nanoparticles (agnps) synthesized using cassia roxburghii plant leaf extract against anopheles stephensi, aedes aegypt ... | 2015 | 26276645 |
| candidate biomarkers for mosquito age-grading identified by label-free quantitative analysis of protein expression in aedes albopictus females. | we applied a "shotgun" approach based on nanoliquid chromatography-high resolution mass spectrometry associated to label free quantification (lfq) to identify proteins varying with age, independently from the physiological state, in aedes albopictus, a mosquito species which in the last decades invaded temperate regions in north america and europe, creating concerns for associated high nuisance and risk of arbovirus transmission. the combined "shotgun" and lfq approach was shown to be highly sui ... | 2015 | 26271156 |
| diversity and abundance of mosquitoes (diptera:culicidae) in an urban park: larval habitats and temporal variation. | urban parks are areas designated for human recreation but also serve as shelter and refuge for populations of several species of native fauna, both migratory and introduced. in brazil, the effect of annual climate variations on aedes aegypti and dengue epidemics in large cities like são paulo is well known, but little is known about how such variations can affect the diversity of mosquito vectors in urban parks and the risk of disease transmission by these vectors. this study investigates the in ... | 2015 | 26259817 |
| effect of ultraviolet-a radiation on the production of leptolegnia chapmanii (saprolegniales: saprolegniaceae) zoospores on dead aedes aegypti (diptera: culicidae) larvae and their larvicidal activity. | impact of uv-radiation in entomopathogens in aquatic environments remains little investigated. the present study reports on the effect of uv-a on the larvicidal activity of leptolegnia chapmanii zoospores in aedes aegypti; on the production of zoospores in larvae killed by the pathogen and then exposed to uv-a; and on the activity of these zoospores against healthy larvae. whereas the virulence of free zoospores in a. aegypti larvae was affected by a uv-a exposure time longer than 10min, product ... | 2015 | 26259676 |
| mosquitocidal and oviposition repellent activities of the extracts of seaweed bryopsis pennata on aedes aegypti and aedes albopictus. | the ever-increasing threat from infectious diseases and the development of insecticide resistance in mosquito populations drive the global search for new natural insecticides. the aims of this study were to evaluate the mosquitocidal activity of the extracts of seaweed bryopsis pennata against dengue vectors aedes aegypti and aedes albopictus, and determine the seaweed's toxic effect on brine shrimp nauplii (as a non-target organism). in addition, the chemical compositions of the active larvicid ... | 2015 | 26247928 |
| effect of chloroxylon swietenia dc bark extracts against culex quinquefasciatus, aedes aegypti, and anopheles stephensi larvae. | mosquitoes are the vector of more diseases and cause major health problems like malaria, dengue, chikungunya, and lymphatic filariasis. this article deals with the mosquito larvicidal activity of chloroxylon swietenia dc bark extracts against late third instar larvae of culex quinquefasciatus, aedes aegypti, and anopheles stephensi. methanolic crude extract of ch. swietenia bark was obtained by soxhlet apparatus and aqueous crude extract by cold percolation method. the range of concentrations of ... | 2015 | 26246308 |
| forced egg retention and oviposition behavior of malaria, dengue and filariasis vectors to a topical repellent diethyl-phenylacetamide. | egg retention and oviposition behavior of four species of mosquito vectors viz., anopheles stephensi, aedes aegypti, aedes albopictus, and culex quinquefasciatus to a topical insect repellent diethyl-phenylacetamide (depa) at 0.1-1000 mg/l was investigated under laboratory conditions. based on oviposition activity indices, depa demonstrated concentration dependent oviposition deterrent effect to a. stephensi (-0.18 to -0.97), a. aegypti (-0.18 to -0.91) and a. albopictus (-0.50 to -0.98) females ... | 2015 | 26245028 |
| plant-borne ovicides in the fight against mosquito vectors of medical and veterinary importance: a systematic review. | mosquitoes (diptera: culicidae) are a huge threat for millions of people worldwide, since they act as vectors for devastating parasites and pathogens. culicidae control is of crucial importance. mosquito eggs, larvae, and pupae are usually targeted using organophosphates, insect growth regulators, and microbial agents. indoor residual spraying and insecticide-treated bed nets are also employed. however, these chemicals have negative effects on human health and the environment, and induce resista ... | 2015 | 26239801 |
| [neonatal dengue. presentation of clinical cases]. | dengue is an infectious disease caused by a flavivirus, with four serotypes, transmitted by the mosquito aedes aegypti. in mexico it is a public health problem, especially in the region of central and southeast of the country. the disease can be asymptomatic or present serious forms and even death. it is confirmed by detection of the ns1 antigen; igm antibodies, polymerase chain reaction and virus isolation. the vertical transmission to de newborn has been little studied. 7 cases in neonates fro ... | 2015 | 26233977 |
| host-feeding pattern of aedes aegypti and aedes albopictus (diptera: culicidae) in heterogeneous landscapes of south andaman, andaman and nicobar islands, india. | mosquito foraging behavior is a determinant of host-vector contact and has an impact on the risk of arboviral epidemics. therefore, blood-feeding patterns is a useful tool for assessing the role in pathogen transmission by vector mosquitoes. competent vectors of dengue and chikungunya viz. aedes aegypti and aedes albopictus are widely prevalent in the andaman and nicobar archipelago. considering the vector potential, medical importance of both these mosquito species and lack of information on ho ... | 2015 | 26220560 |
| tree holes as larval habitats for aedes aegypti in urban, suburban and forest habitats in a dengue affected area. | aedes aegypti (l.) (diptera: culicidae), the main vector of dengue and urban yellow fever in the world, is highly adapted to the human environment. artificial containers are the most common larval habitat for the species, but it may develop in tree holes and other phytotelmata. this study assessed whether tree holes in san ramón de la nueva orán, a city located in subtropical montane moist forest where dengue outbreaks occur, are relevant as larval habitat for ae. aegypti and if the species may ... | 2015 | 26193903 |
| characterization and biotoxicity of hypnea musciformis-synthesized silver nanoparticles as potential eco-friendly control tool against aedes aegypti and plutella xylostella. | two of the most important challenges facing humanity in the 21st century comprise food production and disease control. eco-friendly control tools against mosquito vectors and agricultural pests are urgently needed. insecticidal products of marine origin have a huge potential to control these pests. in this research, we reported a single-step method to synthesize silver nanoparticles (agnp) using the aqueous leaf extract of the seaweed hypnea musciformis, a cheap, nontoxic and eco-friendly materi ... | 2015 | 26184431 |
| evaluation of three commercial backpack sprayers with aqualuer® 20-20 against caged adult aedes aegypti. | three commercially available backpack sprayers were evaluated with aqualuer® 20-20 (20.6% permethrin, active ingredient; 20.6% piperonyl butoxide, technical) against caged adult aedes aegypti in semifield trials in northeastern florida. two battery-powered sprayers, birchmeier and hudson, were compared with the standard hand-pump solo 425 sprayer, which is currently used in pest management operations. physical characteristics, droplet analysis, and overall ease of use were documented. multiple d ... | 2015 | 26181698 |
| a trypsin inhibitor from clitoria fairchildiana cotyledons is active against digestive enzymes of aedes aegypti larvae. | aedes aegypti, the principal mosquito vector of yellow fever, dengue fever and chikungunya fever virus-transmitted diseases, is an insect closely associated with humans and their housing habitats. as there is no commercially available vaccine, prevention is the most suggested form of avoiding disease spreading and a number of studies are being developed in order to give support to vector control operations. the present study reports on the identification of a trypsin inhibitor isolated from coty ... | 2015 | 26156641 |
| evaluation of silver nanoparticles toxicity of arachis hypogaea peel extracts and its larvicidal activity against malaria and dengue vectors. | silver nanoparticles (agnps) were successfully synthesised from aqueous silver nitrate using the extracts of arachis hypogaea peels. the synthesised snps were characterized by fourier transform-infrared spectroscopy analysis, x-ray diffraction, transmission electron microscopy analysis and high-resonance scanning electron microscopy, and energy dispersive x-ray spectroscopy. agnps were well defined and measured 20 to 50 nm in size. the nanoparticles were crystallized with a face-centered cubic s ... | 2015 | 26154036 |
| [microgeographic and temporal genetic changes of aedes aegypti from medellín, colombia]. | aedes aegypti populations may experience changes in abundance and genetic diversity in addition to changes in their evolutionary capability to respond to vector control. the knowledge on the changes in genetic variation on a spatio-temporal scale improves the epidemiological understanding of dengue and supports the appropriate and timely design of vector control strategies. | 2015 | 26148034 |
| [insecticide susceptibility evaluation in aedes aegypti populations of caldas, colombia, in 2007 and 2011]. | continuous use of insecticides for the control of dengue transmission may lead to decreased susceptibility levels in mosquito vector populations. timely monitoring is necessary to ensure detection of any potential resistance problems. | 2015 | 26148033 |
| an environmentally safe larvicide against aedes aegypti based on in situ gelling nanostructured surfactant systems containing an essential oil. | liquid crystalline precursors, which are in situ gelling nanostructured surfactant systems, can undergo phase transition in aqueous solution and become more structured aggregates, controlling release of larvicides and acting as biotechnology alternatives for dengue control. such systems can contain bioactive substances as citrus sinensis essential oil (cseo) which exhibits biological activity against aedes aegypti (ae. aegypti) larvae. | 2015 | 26125515 |
| susceptibility status of aedes aegypti (l.) (diptera: culicidae) to temephos from three districts of tamil nadu, india. | dengue is the most rapidly expanding arboviral disease in india. aedes aegypti is the primary vector of dengue fever. chemical insecticides have long been used in the vector control programmes along with other control measures. however, continuous use of insecticides targeting ae. aegypti may lead to development of insecticide resistance. though resistance in ae. aegypti has been reported, the mutation in ace-1 gene associated with temephos resistance is not reported in natural populations. the ... | 2015 | 26119549 |
| boric acid ovicidal trap for the management of aedes species. | the use of low concentrations of boric acid as a potential and effective control agent for the eggs and immature stages of aedes aegypti l. and aedes albopictus skuse (diptera: culicidae) is found to be safe and effective as compared to synthetic chemical insecticides. the study aims to determine the ovicidal concentration of boric acid, its effective concentration for oviposition attraction and the larval mortality concentration for ae. aegypti and ae. albopictus. | 2015 | 26119547 |
| eco-friendly control of malaria and arbovirus vectors using the mosquitofish gambusia affinis and ultra-low dosages of mimusops elengi-synthesized silver nanoparticles: towards an integrative approach? | mosquito-borne diseases represent a deadly threat for millions of people worldwide. however, the use of synthetic insecticides to control culicidae may lead to high operational costs and adverse non-target effects. plant-borne compounds have been proposed for rapid extracellular synthesis of mosquitocidal nanoparticles. their impact against biological control agents of mosquito larval populations has been poorly studied. we synthesized silver nanoparticles (agnp) using the aqueous leaf extract o ... | 2015 | 26300364 |
| further evidences for the mode of action of the larvicidal m-pentadecadienyl-phenol isolated from myracrodruon urundeuva seeds against aedes aegypti. | nowadays, dengue fever is considered the most important arbovirosis worldwide and its control is still based upon combating the vector aedes aegypti. besides monitoring of mosquito populations resistant to conventional insecticides, the search for new environmentally safe insecticides and conduction of molecular studies focusing on the elucidation of mode of action and possible resistance mechanisms are considered the key for a sustainable management of the mosquito vector. thus, the present wor ... | 2015 | 26299195 |
| widespread evidence for interspecific mating between aedes aegypti and aedes albopictus (diptera: culicidae) in nature. | aedes aegypti and aedes albopictus, two important vectors of the dengue and chikungunya viruses to humans, often come in contact in their invasive ranges. in these circumstances, a number of factors are thought to influence their population dynamics, including resource competition among the larval stages, prevailing environmental conditions and reproductive interference in the form of satyrization. as the distribution and abundance of ae. aegypti and ae. albopictus have profound epidemiological ... | 2015 | 26296606 |
| an in vitro bioassay for the quantitative evaluation of mosquito repellents against stegomyia aegypti (=aedes aegypti) mosquitoes using a novel cocktail meal. | to assess the efficacy of new insect repellents, an efficient and safe in vitro bioassay system using a multiple-membrane blood-feeding device and a cocktail meal was developed. the multiple-membrane blood-feeding device facilitates the identification of new insect repellents by the high-throughput screening of candidate chemicals. a cocktail meal was developed as a replacement for blood for feeding females of stegomyia aegypti (=aedes aegypti) (l.) (diptera: culicidae). the cocktail meal consis ... | 2015 | 25828787 |
| cymbopogon citratus-synthesized gold nanoparticles boost the predation efficiency of copepod mesocyclops aspericornis against malaria and dengue mosquitoes. | plant-borne compounds can be employed to synthesize mosquitocidal nanoparticles that are effective at low doses. however, how they affect the activity of mosquito predators in the aquatic environment is unknown. in this study, we synthesized gold nanoparticles (aun) using the leaf extract of cymbopogon citratus, which acted as a reducing and capping agent. aun were characterized by a variety of biophysical methods and sorted for size in order to confirm structural integrity. c. citratus extract ... | 2015 | 25819295 |
| extract of bowdichia virgilioides and maackiain as larvicidal agent against aedes aegypti mosquito. | the larvicidal activities of extracts of three hardwood species (hymenaea stigonorcapa, anadenanthera colubrina and bowdichia virgilioides) against 4th instar larvae of aedes aegypti were evaluated using who guidelines. extracts of h. stignocarpa and a. colubrina showed weak activity. the highest larvicidal effect was obtained with the cyclohexane extract of the heartwood of b. virgilioides, which caused 100% mortality at concentrations at 50 and 100 µg/ml. fraction toluene/etoac (8:2) from this ... | 2015 | 25819294 |
| effect of salinity on the behavior of aedes aegypti populations from the coast and plateau of southeastern brazil. | mosquito larvae can develop in fresh, salty and brackish water. the larvae of aedes aegypti develop in fresh water. however, in laboratory studies, tolerance of this species for oviposition and hatching in brackish water was observed. immature forms of ae. aegypti have also been found developing in brackish water in coastal areas. the purpose of the study was to compare the effects of salt on the biological cycle of ae. aegypti populations from coastal and plateau areas of southeastern brazil. | 2015 | 25815871 |
| [assessment of the risk of expansion of the habitats of the mosquitoes aedes aegypti l. and aedes albopictus skus. in russia]. | based on climatic characteristics, the authors show that an invasive aedes albopictus species is likely to emerge in new areas of the russian federation. | 2015 | 25812400 |
| an entomological review of invasive mosquitoes in europe. | among the invasive mosquitoes registered all over the world, aedes species are particularly frequent and important. as several of them are potential vectors of disease, they present significant health concerns for 21st century europe. five species have established in mainland europe, with two (aedes albopictus and aedes japonicus) becoming widespread and two (ae. albopictus and aedes aegypti) implicated in disease transmission to humans in europe. the routes of importation and spread are often e ... | 2015 | 25804287 |
| low efficacy of delthamethrin-treated net against singapore aedes aegypti is associated with kdr-type resistance. | there has been a worldwide surge in the number and severity of dengue in the past decades. in singapore, relentless vector control efforts have been put in to control the disease since the 1960's. space spraying, fogging, chemical treatment and source reduction are some commonly used methodologies for controlling its vectors, particularly aedes aegypti. here, as we explored the use of a commercially available delthamethrin-treated net as an alternative strategy and the efficacy of the treated ne ... | 2015 | 25801264 |
| an investigation on the diversity of mosquitocidal bacteria and its relationship with incidence of vector borne diseases. | control of mosquitoes is the most important aspect of public health, as mosquitoes transmit many human diseases, including the fatal infection, japanese encephalitis. this paper addresses the isolation of new mosquitocidal bacteria from soil samples in the union territory of pondicherry, india, where, no clinical cases of vector borne infections have been reported. bacterial isolates from soil samples were screened for potential mosquitocidal strains and bioassays against mosquito vectors (culex ... | 2015 | 25801257 |
| evaluation of sumithion l-40 against aedes aegypti (l.) and aedes albopictus skuse. | space spraying of chemical insecticides is still an important mean of controlling aedes mosquitoes and dengue transmission. for this purpose, the bioefficacy of space-sprayed chemical insecticide should be evaluated from time to time. a simulation field trial was conducted outdoor in an open field and indoor in unoccupied flat units in kuala lumpur, to evaluate the adulticidal and larvicidal effects of sumithion l-40, a ulv formulation of fenitrothion. a thermal fogger with a discharge rate of 2 ... | 2015 | 25801256 |
| feeding stage, species, body part and sex-specific activity of glutathione s-transferase in mosquito. | in the present study, the feeding stage, body parts, development and sex specific activity of glutathione s-transferases (gsts) were observed in different mosquito species (aedes aegypti, culex quinquefasciatus, anopheles stephensi, an. culicifacies, an. annularis, an. subpictus, an. vagus). gst activity was assayed spectrophotometrically at 23°c, using a uv max microplate reader, to measure the rate of conjugation of gsh to cdnb. a significant species-specific difference in the activity of gst ... | 2015 | 25801255 |
| surveillance of aedes albopictus skuse breeding preference in selected dengue outbreak localities, peninsular malaysia. | entomological surveillance was conducted in order to determine the abundance and to evaluate any changes of biological vectors or ecology, especially in the dengue outbreak areas. the abundance and breeding preference of aedes albopictus and aedes aegypti were conducted in selected dengue outbreak localities in three states of peninsular malaysia namely selangor, federal territory of kuala lumpur, and penang island using ovitraps and larval survey method. it was determined that ae. albopictus wa ... | 2015 | 25801254 |
| nectar protein content and attractiveness to aedes aegypti and culex pipiens in plants with nectar/insect associations. | we chose five easily propagated garden plants previously shown to be attractive to mosquitoes, ants or other insects and tested them for attractiveness to culex pipiens and aedes aegypti. long term imbibition was tested by survival on each plant species. both mosquito species survived best on impatiens walleriana, the common garden impatiens, followed by asclepias curassavica, campsis radicans and passiflora edulis, which sponsored survival as well as the 10% sucrose control. immediate preferenc ... | 2015 | 25792420 |
| is there a risk of chikungunya transmission in canada? | an outbreak of chikungunya virus infection is currently underway in the caribbean, central america and south america, and autochthonous (local or indigenous) transmission has occurred in the southeastern united states. the mosquito species known to transmit chikungunya-aedes aegypti and aedes albopictus-are not known to reside in canada at this time. but how comfortable can we be that this situation will continue? here we explore four key conditions that must be met for transmission of chikungun ... | 2015 | 29769915 |
| characterization of a gene family encoding sea (sea-urchin sperm protein, enterokinase and agrin)-domain proteins with lectin-like and heme-binding properties from schistosoma japonicum. | we previously identified a novel gene family dispersed in the genome of schistosoma japonicum by retrotransposon-mediated gene duplication mechanism. although many transcripts were identified, no homolog was readily identifiable from sequence information. | 2014 | 24416467 |
| mass production of genetically modified aedes aegypti for field releases in brazil. | new techniques and methods are being sought to try to win the battle against mosquitoes. recent advances in molecular techniques have led to the development of new and innovative methods of mosquito control based around the sterile insect technique (sit)(1-3). a control method known as ridl (release of insects carrying a dominant lethal)(4), is based around sit, but uses genetic methods to remove the need for radiation-sterilization(5-8). a ridl strain of ae. aegypti was successfully tested in t ... | 2014 | 24430003 |
| the potential role of as-sumo-1 in the embryonic diapause process and early embryo development of artemia sinica. | during embryonic development of artemia sinica, environmental stresses induce the embryo diapause phenomenon, required to resist apoptosis and regulate cell cycle activity. the small ubiquitin-related modifier-1 (sumo), a reversible post-translational protein modifier, plays an important role in embryo development. sumo regulates multiple cellular processes, including development and other biological processes. the molecular mechanism of diapause, diapause termination and the role of as-sumo-1 i ... | 2014 | 24404204 |
| accuracy of microrna discovery pipelines in non-model organisms using closely related species genomes. | mapping small reads to genome reference is an essential and more common approach to identify micrornas (mirnas) in an organism. using closely related species genomes as proxy references can facilitate mirna expression studies in non-model species that their genomes are not available. however, the level of error this introduces is mostly unknown, as this is the result of evolutionary distance between the proxy reference and the species of interest. to evaluate the accuracy of mirna discovery pipe ... | 2014 | 24404190 |
| reduction in dengue cases observed during mass control of aedes (stegomyia) in street catch basins in an endemic urban area in colombia. | dengue incidence continues to increase globally and, in the absence of an efficacious vaccine, prevention strategies are limited to vector control. it has been suggested that targeting the most productive breeding sites instead of all water-holding containers could be a cost-effective vector control strategy. we sought to identify and continuously control the most productive aedes (stegomyia) breeding site in an endemic urban area in colombia and followed the subsequent incidence of dengue. in t ... | 2014 | 24388794 |
| identification and expression analysis of diapause hormone and pheromone biosynthesis activating neuropeptide (dh-pban) in the legume pod borer, maruca vitrata fabricius. | neuropeptides play essential roles in a variety of physiological responses that contribute to the development and reproduction of insects. both the diapause hormone (dh) and pheromone biosynthesis activating neuropeptide (pban) belong to the pban/pyrokinin neuropeptide family, which has a conserved pentapeptide motif fxprl at the c-terminus. we identified the full-length cdna encoding dh-pban in maruca vitrata, a major lepidopteran pest of leguminous crops. the open reading frame of marvi-dh-pba ... | 2014 | 24409312 |
| a steroid receptor coactivator acts as the dna-binding partner of the methoprene-tolerant protein in regulating juvenile hormone response genes. | methoprene-tolerant (met) protein is a juvenile hormone (jh) receptor in insects. jh-bound met forms a complex with the βftz-f1-interacting steroid receptor coactivator (fisc) and together they regulate jh response genes in mosquitoes. both proteins contain basic helix-loop-helix (bhlh) and pas motifs. here we demonstrated that fisc is the obligatory partner of met for binding to jh-response elements (jhres). met or fisc alone could not bind a previously characterized jhre, while formation of th ... | 2014 | 25004255 |
| toxicity of six plant extracts and two pyridone alkaloids from ricinus communis against the malaria vector anopheles gambiae. | the african malaria vector, anopheles gambiae s.s., is known to feed selectively on certain plants for sugar sources. however, the adaptive significance of this behaviour especially on how the extracts of such plants impact on the fitness of this vector has not been explored. this study determined the toxicity and larvicidal activity on this vector of extracts from six selected plants found in kenya and two compounds identified from ricinus communis: 3-carbonitrile-4-methoxy-n-methyl-2-pyridone ... | 2014 | 24996560 |
| feasible introgression of an anti-pathogen transgene into an urban mosquito population without using gene-drive. | introgressing anti-pathogen constructs into wild vector populations could reduce disease transmission. it is generally assumed that such introgression would require linking an anti-pathogen gene with a selfish genetic element or similar technologies. yet none of the proposed transgenic anti-pathogen gene-drive mechanisms are likely to be implemented as public health measures in the near future. thus, much attention now focuses instead on transgenic strategies aimed at mosquito population suppres ... | 2014 | 24992213 |
| effects of insemination and blood-feeding on locomotor activity of aedes albopictus and aedes aegypti (diptera: culicidae) females under laboratory conditions. | dengue is an arbovirus disease transmitted by two aedes mosquitoes: ae. aegypti and ae. albopictus. virgin females of these two species generally show a bimodal and diurnal pattern of activity, with early morning and late afternoon peaks. although some studies on the flight activity of virgin, inseminated and blood-fed ae. aegypti females have been carried out under laboratory conditions, little is known about the effects of such physiological states on the locomotor activity of ae. albopictus a ... | 2014 | 24990394 |
| larval competition extends developmental time and decreases adult size of wmelpop wolbachia-infected aedes aegypti. | the intracellular endosymbiont wolbachia has been artificially transinfected into the dengue vector aedes aegypti, where it is being investigated as a potential dengue biological control agent. invasion of wolbachia in natural populations depends upon the fitness of wolbachia-infected ae. aegypti relative to uninfected competitors. although wolbachia infections impose fitness costs on the adult host, effects at the immature stages are less clear, particularly in competitive situations. we look f ... | 2014 | 24732463 |
| myocarditis in a traveler returning from the dominican republic: an unusual presentation of dengue fever. | myocarditis is an uncommon manifestation of dengue fever. we describe a case of a 69-year-old hispanic male who presented to an emergency room in new york city 3 days after returning from a trip to the dominican republic complaining of a 1-day history of chest pain and fever. his first electrocardiogram showed a new left bundle branch block, and initial cardiac enzymes included troponin of 5 ng/dl, creatine kinase-mb of 9 ng/ml, and myoglobin of 234 ng/ml. dengue fever antibodies were found to b ... | 2014 | 24891462 |
| dengue virus in bats from southeastern mexico. | to identify the relationship between landscape use and dengue virus (denv) occurrence in bats, we investigated the presence of denv from anthropogenically changed and unaltered landscapes in two biosphere reserves: calakmul (campeche) and montes azules (chiapas) in southern mexico. spleen samples of 146 bats, belonging to 16 species, were tested for four denv serotypes with standard reverse transcriptase polymerase chain reaction (rt-pcr) protocols. six bats (4.1%) tested positive for denv-2: fo ... | 2014 | 24752688 |
| disruption of aminergic signalling reveals novel compounds with distinct inhibitory effects on mosquito reproduction, locomotor function and survival. | insecticide resistance amongst disease vectors is a growing problem and novel compounds are needed. biogenic amines are important for neurotransmission and we have recently shown a potential role for these in mosquito fertility. here, we dissected the relative contribution of different aminergic signalling pathways to biological processes essential for vectorial capacity such as fertility, locomotion and survival by injecting agonists and antagonists and showed that octopaminergic/tyraminergic s ... | 2014 | 24984706 |
| beyond sex allocation: the role of mating systems in sexual selection in parasitoid wasps. | despite the diverse array of mating systems and life histories which characterise the parasitic hymenoptera, sexual selection and sexual conflict in this taxon have been somewhat overlooked. for instance, parasitoid mating systems have typically been studied in terms of how mating structure affects sex allocation. in the past decade, however, some studies have sought to address sexual selection in the parasitoid wasps more explicitly and found that, despite the lack of obvious secondary sexual t ... | 2014 | 24981603 |
| origin of the dengue fever mosquito, aedes aegypti, in california. | dengue fever is among the most widespread vector-borne infectious diseases. the primary vector of dengue is the aedes aegypti mosquito. ae. aegypti is prevalent in the tropics and sub-tropics and is closely associated with human habitats outside its native range of africa. while long established in the southeastern united states of america where dengue is re-emerging, breeding populations have never been reported from california until the summer of 2013. using 12 highly variable microsatellite l ... | 2014 | 25077804 |
| introducing a dengue vaccine to mexico: development of a system for evidence-based public policy recommendations. | 2014 | 25077478 | |
| role of methoprene-tolerant (met) in adult morphogenesis and in adult ecdysis of blattella germanica. | juvenile hormone (jh) represses metamorphosis of young instars in insects. one of the main players in hormonal signalling is methoprene-tolerant (met), which plays the role of jh receptor. using the polyneopteran insect blattella germanica as the model and rnai for transcript depletion, we have confirmed that met transduces the antimetamorphic signal of jh in young nymphs and plays a role in the last nymphal instar moult in this species. previously, the function of met as the jh receptor had bee ... | 2014 | 25072526 |
| pigment dispersing factor regulates ecdysone biosynthesis via bombyx neuropeptide g protein coupled receptor-b2 in the prothoracic glands of bombyx mori. | ecdysone is the key hormone regulating insect growth and development. ecdysone synthesis occurs in the prothoracic glands (pgs) and is regulated by several neuropeptides. four prothoracicotropic and three prothoracicostatic factors have been identified to date, suggesting that ecdysone biosynthesis is intricately regulated. here, we demonstrate that the neuropeptide pigment dispersing factor (pdf) stimulates ecdysone biosynthesis and that this novel signaling pathway partially overlaps with the ... | 2014 | 25072638 |
| challenges encountered using standard vector control measures for dengue in boa vista, brazil. | in 2010, dengue virus (denv) serotype-4 was detected during a dengue outbreak in the amazonian city of boa vista. at that time brazil was already endemic for denv-1, denv-2 and denv-3. this was the first time denv-4 was observed in the country after it was initially detected and eliminated in 1981. | 2014 | 25378760 |
| dengue and the world football cup: a matter of timing. | 2014 | 25058369 | |
| aedes cadherin mediates the in vivo toxicity of the cry11aa toxin to aedes aegypti. | cadherin plays an important role in the toxicity of bacillus thuringiensis cry proteins. we previously cloned a full-length cadherin from aedes aegypti larvae and reported this protein binds cry11aa toxin from b. thuringiensis subsp. israelensis with high affinity, ≈16.7nm. based on these results, we investigated if aedes cadherin is involved in the in vivo toxicity of cry11aa toxin to ae. aegypti. we established a mosquito cell line stably expressing the full-length aedes cadherin and transgeni ... | 2014 | 25064814 |
| excretion of nacl and kcl loads in mosquitoes. 2. effects of the small molecule kir channel modulator vu573 and its inactive analog vu342. | the effect of two small molecules vu342 and vu573 on renal functions in the yellow fever mosquito aedes aegypti was investigated in vitro and in vivo. in isolated malpighian tubules, vu342 (10 μm) had no effect on the transepithelial secretion of na(+), k(+), cl(-), and water. in contrast, 10 μm vu573 first stimulated and then inhibited the transepithelial secretion of fluid when the tubules were bathed in na(+)-rich or k(+)-rich ringer solution. the early stimulation was blocked by bumetanide, ... | 2014 | 25056106 |
| medusa: a novel gene drive system for confined suppression of insect populations. | gene drive systems provide novel opportunities for insect population suppression by driving genes that confer a fitness cost into pest or disease vector populations; however regulatory issues arise when genes are capable of spreading across international borders. gene drive systems displaying threshold properties provide a solution since they can be confined to local populations and eliminated through dilution with wild-types. we propose a novel, threshold-dependent gene drive system, medusa, ca ... | 2014 | 25054803 |
| exploratory space-time analysis of dengue incidence in trinidad: a retrospective study using travel hubs as dispersal points, 1998-2004. | dengue is an acute arboviral disease responsible for most of the illness and death in tropical and subtropical regions. over the last 25 years there has been increase epidemic activity of the disease in the caribbean, with the co-circulation of multiple serotypes. an understanding of the space and time dynamics of dengue could provide health agencies with important clues for reducing its impact. | 2014 | 25052242 |
| identification and characterisation of aedes aegypti aldehyde dehydrogenases involved in pyrethroid metabolism. | pyrethroid insecticides, especially permethrin and deltamethrin, have been used extensively worldwide for mosquito control. however, insecticide resistance can spread through a population very rapidly under strong selection pressure from insecticide use. the upregulation of aldehyde dehydrogenase (aldh) has been reported upon pyrethroid treatment. in aedes aegypti, the increase in aldh activity against the hydrolytic product of pyrethroid has been observed in ddt/permethrin-resistant strains. th ... | 2014 | 25047125 |
| molecular phylogeny and predicted 3d structure of plant beta-d-n-acetylhexosaminidase. | beta-d-n-acetylhexosaminidase, a family 20 glycosyl hydrolase, catalyzes the removal of β-1,4-linked n-acetylhexosamine residues from oligosaccharides and their conjugates. we constructed phylogenetic tree of β-hexosaminidases to analyze the evolutionary history and predicted functions of plant hexosaminidases. phylogenetic analysis reveals the complex history of evolution of plant β-hexosaminidase that can be described by gene duplication events. the 3d structure of tomato β-hexosaminidase (β-h ... | 2014 | 25165734 |
| household use of insecticide consumer products in a dengue-endemic area in méxico. | to evaluate the household use of insecticide consumer products to kill mosquitoes and other insect pests, as well as the expenditures for using these products, in a dengue-endemic area of méxico. | 2014 | 25040259 |
| long-term and seasonal dynamics of dengue in iquitos, peru. | long-term disease surveillance data provide a basis for studying drivers of pathogen transmission dynamics. dengue is a mosquito-borne disease caused by four distinct, but related, viruses (denv-1-4) that potentially affect over half the world's population. dengue incidence varies seasonally and on longer time scales, presumably driven by the interaction of climate and host susceptibility. precise understanding of dengue dynamics is constrained, however, by the relative paucity of laboratory-con ... | 2014 | 25033412 |
| subdoses of 17dd yellow fever vaccine elicit equivalent virological/immunological kinetics timeline. | the live attenuated 17dd yellow fever vaccine is one of the most successful prophylactic interventions for controlling disease expansion ever designed and utilized in larger scale. however, increase on worldwide vaccine demands and manufacturing restrictions urge for more detailed dose sparing studies. the establishment of complementary biomarkers in addition to prnt and viremia could support a secure decision-making regarding the use of 17dd yf vaccine subdoses. the present work aimed at compar ... | 2014 | 25022840 |
| intraspecific variation of centruroides edwardsii venom from two regions of colombia. | we report the first description studies, partial characterization, and intraspecific difference of centruroides edwardsii, gervais 1843, venom. c. edwardsii from two colombian regions (antioquia and tolima) were evaluated. both venoms showed hemolytic activity, possibly dependent of enzymatic active phospholipases, and neither coagulant nor proteolytic activities were observed. venom electrophoretic profile showed significant differences between c. edwardsii venom from both regions. a high conce ... | 2014 | 25025710 |
| predicting local dengue transmission in guangzhou, china, through the influence of imported cases, mosquito density and climate variability. | each year there are approximately 390 million dengue infections worldwide. weather variables have a significant impact on the transmission of dengue fever (df), a mosquito borne viral disease. df in mainland china is characterized as an imported disease. hence it is necessary to explore the roles of imported cases, mosquito density and climate variability in dengue transmission in china. the study was to identify the relationship between dengue occurrence and possible risk factors and to develop ... | 2014 | 25019967 |
| apropos: an update on the incidence of dengue gaining strength in saudi arabia and current control approaches for its vector mosquito. | 2014 | 25015720 | |
| insecticide resistance status of united states populations of aedes albopictus and mechanisms involved. | aedes albopictus (skuse) is an invasive mosquito that has become an important vector of chikungunya and dengue viruses. immature ae. albopictus thrive in backyard household containers that require treatment with larvicides and when adult populations reach pest levels or disease transmission is ongoing, adulticiding is often required. to assess the feasibility of control of usa populations, we tested the susceptibility of ae. albopictus to chemicals representing the main insecticide classes with ... | 2014 | 25013910 |
| chemosensory receptors in tsetse flies provide link between chemical and behavioural ecology. | tsetse flies survive in a variety of environments across tropical africa, often rising to large numbers, despite their low birth rate of one offspring every seven to nine days. they use olfactory receptors to process chemical signals in their environments to find food, escape from predators, and locate suitable larviposition sites. we discuss the identification of odorant and gustatory receptors in glossina morsitans morsitans and the role genomics could play in management of nuisance insects. | 2014 | 25017128 |
| a serine protease homolog negatively regulates tep1 consumption in systemic infections of the malaria vector anopheles gambiae. | clip domain serine protease homologs are widely distributed in insect genomes and play important roles in regulating insect immune responses, yet their exact functions remain poorly understood. here, we show that clipa2, a clip domain serine protease homolog of anopheles gambiae, regulates the consumption of the mosquito complement-like protein tep1 during systemic bacterial infections. we provide evidence that clipa2 localizes to microbial surfaces in a tep1-dependent manner whereby it negative ... | 2014 | 25012124 |
| hmgb1 in health and disease. | complex genetic and physiological variations as well as environmental factors that drive emergence of chromosomal instability, development of unscheduled cell death, skewed differentiation, and altered metabolism are central to the pathogenesis of human diseases and disorders. understanding the molecular bases for these processes is important for the development of new diagnostic biomarkers, and for identifying new therapeutic targets. in 1973, a group of non-histone nuclear proteins with high e ... | 2014 | 25010388 |
| the pseudo gtpase cenp-m drives human kinetochore assembly. | kinetochores, multi-subunit complexes that assemble at the interface with centromeres, bind spindle microtubules to ensure faithful delivery of chromosomes during cell division. the configuration and function of the kinetochore-centromere interface is poorly understood. we report that a protein at this interface, cenp-m, is structurally and evolutionarily related to small gtpases but is incapable of gtp-binding and conformational switching. we show that cenp-m is crucially required for the assem ... | 2014 | 25006165 |
| malsoor virus, a novel bat phlebovirus, is closely related to severe fever with thrombocytopenia syndrome virus and heartland virus. | during a survey in the year 2010, a novel phlebovirus was isolated from the rousettus leschenaultii species of bats in western india. the virus was identified by electron microscopy from infected vero e6 cells. phylogenic analysis of the complete genome showed its close relation to severe fever with thrombocytopenia syndrome (sfts) and heartland viruses, which makes it imperative to further study its natural ecology and potential as a novel emerging zoonotic virus. | 2014 | 24390329 |
| exposure to diflubenzuron results in an up-regulation of a chitin synthase 1 gene in citrus red mite, panonychus citri (acari: tetranychidae). | chitin synthase synthesizes chitin, which is critical for the arthropod exoskeleton. in this study, we cloned the cdna sequences of a chitin synthase 1 gene, pcchs1, in the citrus red mite, panonychus citri (mcgregor), which is one of the most economically important pests of citrus worldwide. the full-length cdna of pcchs1 contains an open reading frame of 4605 bp of nucleotides, which encodes a protein of 1535 amino acid residues with a predicted molecular mass of 175.0 kda. a phylogenetic anal ... | 2014 | 24590130 |
| the secreted triose phosphate isomerase of brugia malayi is required to sustain microfilaria production in vivo. | human lymphatic filariasis is a major tropical disease transmitted through mosquito vectors which take up microfilarial larvae from the blood of infected subjects. microfilariae are produced by long-lived adult parasites, which also release a suite of excretory-secretory products that have recently been subject to in-depth proteomic analysis. surprisingly, the most abundant secreted protein of adult brugia malayi is triose phosphate isomerase (tpi), a glycolytic enzyme usually associated with th ... | 2014 | 24586152 |
| specific b-cell epitope of per a 1: a major allergen of american cockroach (periplaneta americana) and anatomical localization. | cockroach (cr) is a common source of indoor allergens, and per a 1 is a major american cr (periplaneta americana) allergen; however, several attributes of this protein remain unknown. this study identifies a novel specific b cell epitope and anatomical locations of per a 1.0105. | 2014 | 24991456 |