Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| complement resistance of pathogenic entamoeba histolytica mediated by trypsin-sensitive surface component(s). | pathogenic forms of the protozoan parasite entamoeba histolytica were reported previously to resist the cytolytic effect of the alternative complement pathway (ap) only temporarily during exposure to complement. in contrast, nonpathogenic forms of e. histolytica had been found to show ap resistance as a stable property. we studied the mechanisms of ap resistance of the two forms. upon exposure to ap activity, resistant pathogenic or nonpathogenic forms bound significantly less c3 products than c ... | 1993 | 8478051 |
| use of a high molecular weight entamoeba histolytica antigen fraction in enzyme-linked immunosorbent assay and thin layer immunoassay tests. | a high molecular weight entamoeba histolytica antigen fraction i was employed for the determination of anti-amoebic antibodies in elisa and thin layer immunoassay (tia) tests. a large number of human serum and immunized guinea pig serum samples were tested against this antigen. in comparison, elisa was found more sensitive than the tia technique. the potent antigen fraction i used in these tests was lyophilized at an optimum concentration of 1 mg/ml for obtaining uniform results. | 1993 | 8481651 |
| nucleotide sequence analysis of the rrna transcription unit of a pathogenic entamoeba histolytica strain hm-1:imss. | 1993 | 8493112 | |
| entamoeba histolytica cysts with a defective wall formed under axenic conditions. | axenic hk9 entamoeba histolytica strain amoebae, maintained in pehs medium, displayed several cystic characteristics that involve an active process of cystic wall formation, cellular volume and density diminution, and one or two nuclear divisions. the differentiation process was asynchronic, beginning after the logarithmic growth phase. the axenic cysts, which were maintained in a 50 mosm/kg medium at 4 degrees c for 72 h, produced growing trophozoites within 1-7 days of incubation at 36 degrees ... | 1993 | 8493243 |
| a human cdna corresponding to a gene overexpressed during cell proliferation encodes a product sharing homology with amoebic and bacterial proteins. | a clone, designated pag, was isolated by differential screening of cdna libraries made from the untransformed and ras-transformed human mammary epithelial cell line hbl100. this cdna corresponds to a gene constitutively expressed in most human cells which is induced to higher levels upon serum stimulation in untransformed and ras-transformed hbl100 cells. however, the abundance of the pag transcript is approximately 3-fold higher in transformed as compared to untransformed cells after 7-15 h of ... | 1993 | 8496166 |
| entamoeba histolytica trophozoites: a surface-associated cysteine protease. | the purpose of this work was to define cell surface proteases on entamoeba histolytica trophozoites. molecular sizes of 2-mercaptoethanol-activated proteases were determined in several ameba cell fractions. inhibited proteases were resolved in sds-polyacrylamide gels and then defined by their ability to digest bovine albumin during electrophoretic migration of ameba bands through a stacking gel containing albumin. this second gel revealed nine gaps of digestion along the horizontal albumin line ... | 1993 | 8500583 |
| cloning of the eh cdc2 gene from entamoeba histolytica encoding a protein kinase p34cdc2 homologue. | to understand better growth regulation in the protozoan parasite, entamoeba histolytica (eh), a homologue of the cdc2 gene encoding the yeast cyclin-dependent protein kinase, p34cdc2, has been cloned and sequenced. this gene, called eh cdc2, contains a 79-bp intron located in the same place as the second of four introns in the schizosaccharomyces pombe cdc2 gene. the sequence of an eh cdc2 cdna confirms the conserved eukaryotic splice donor (gt) and acceptor (ag) sites and shows that eh is able ... | 1993 | 8500762 |
| in vitro effect of 5-hydroxytryptamine on concanavalin-a agglutinability and multiplication of entamoeba histolytica. | six isolates of e. histolytica isolated and maintained by serial passage in modified boeck and drbohlav's medium were used in this study. when five of the isolates were grown in the above medium with 5-hydroxytryptamine (5-ht) added to the overlay, the agglutinability of the amoebae by concanavalin- a (con-a) was significantly increased compared to corresponding control cultures. four isolates of e. histolytica grown with 5-ht had 1.5 to 2 times higher counts than the control cultures. similarly ... | 1993 | 8500845 |
| trichuris vulpis infection in an indian tribal population. | stools from 28 of the 82 inhabitants on remote little andaman island in india were examined for parasite eggs and cysts. trichuris trichiura eggs were found in 27, trichuris vulpis eggs in 5, strongyloides stercoralis larvae in 3, hookworm eggs in 15, entamoeba histolytica and entamoeba coli cysts each in 9, giardia lamblia in 6, retortamonas sp. in 3, iodoamoeba sp. in 2, and chilomastix sp. in 2 stools. ascaris lumbricoides eggs were not seen. the occurrence of t. vulpis eggs in 5 stools and t ... | 1993 | 8501609 |
| ulcerative colitis and antineutrophil cytoplasmic antibodies in hong kong chinese. | inflammatory bowel diseases are known to be rare among the chinese. the diagnosis of ulcerative colitis has been difficult in some of the asian countries where infective colitis is more prevalent. twenty-three hong kong chinese patients diagnosed to have ulcerative colitis were reviewed. the symptoms were relatively mild and extraintestinal manifestation had been rare. patients responded well to steroid therapy and sulfasalazine. three patients in this series were found to have cyst and/or troph ... | 1993 | 8503382 |
| induction of complement resistance in cloned pathogenic entamoeba histolytica. | the lytic effect of complement activated through the alternative pathway (ap) was studied on pathogenic and nonpathogenic entamoeba histolytica recently isolated from stool samples. recent nonpathogenic isolates were nearly unaffected by exposure to ap whereas recent pathogenic stool isolates were highly susceptible to ap dependent complement-mediated lysis. complement susceptible pathogenic stool isolates developed complement resistance in vivo during hamster liver passage and in vitro during c ... | 1993 | 8506118 |
| quassinoids exhibit greater selectivity against plasmodium falciparum than against entamoeba histolytica, giardia intestinalis or toxoplasma gondii in vitro. | the in vitro activities of a series of quassinoids against plasmodium falciparum, entamoeba histolytica, giardia intestinalis and toxoplasma gondii have been compared with their in vitro cytotoxic effects against kb cells (human epidermoid carcinoma of the nasopharynx). all of the compounds tested were more toxic to kb cells than to g. intestinalis, but four (ailanthinone, bruceine d, brusatol and glaucarubinone) were slightly less toxic to kb cells than to e. histolytica. glaucarubinone was sim ... | 1993 | 8508162 |
| a redescription of entamoeba histolytica schaudinn, 1903 (emended walker, 1911) separating it from entamoeba dispar brumpt, 1925. | explaining the low incidence of invasive disease (10%) in humans infected with entamoeba histolytica has occupied the attention of generations of both clinical and nonclinical investigators. one possible explanation would be the existence of two morphologically identical species-one an invasive pathogen, the other noninvasive. this was first proposed by brumpt in 1925, but his explanation was virtually ignored until 1978 when the first of several publications appeared suggesting that e. histolyt ... | 1993 | 8508172 |
| comparison of pore-forming peptides from pathogenic and nonpathogenic entamoeba histolytica. | similar to the findings obtained with pathogenic entamoeba histolytica, nonpathogenic isolates were found to kill mammalian cells in vitro, and cell extract caused pore formation in liposome membranes. a pore-forming peptide termed apnp was isolated from a nonpathogenic isolate using the schedule developed for the purification of app or amoebapore, the homologous peptide of the pathogenic isolate hm-1:imss. compared to app, the specific activity of apnp in pore formation was 60% lower. cdna sequ ... | 1993 | 8515772 |
| identification and characterization of a myosin heavy chain gene (mhca) from the human parasitic pathogen entamoeba histolytica. | the mhca gene from the human pathogen entamoeba histolytica was identified using the polymerase chain reaction. it is a single copy gene expressed as a 6.4-kb mrna. the deduced mhca protein sequence is highly similar to myosin ii from both dictyostelium discoideum and acanthamoeba castellanii. the globular head domain of mhca contains the specific regions involved in atp binding, actin binding, and interaction with myosin light chain. the tail domain is organized in an alpha-helical coiled coil ... | 1993 | 8515774 |
| molecular karyotype of related clones of entamoeba histolytica. | the molecular karyotype of 3 clones derived from strain hm1:imss of entamoeba histolytica was studied by transverse alternating field electrophoresis. 11-20 bands ranging between 0.3 and over 3 mb were resolved. hybridization with total dna detected highly repetitive sequences in the slow-migrating molecules, while non-repetitive sequences were located in the intermediate and fast-migrating molecules. rdna, tubulin, actin, cysteine proteases dna fragments, and a variable dna sequence (ehvr1) loc ... | 1993 | 8515781 |
| a family of transcripts (k2) of entamoeba histolytica contains polymorphic repetitive regions with highly conserved elements. | sera from patients with invasive amebiasis were used to identify a cdna clone (k2p-1) encoding a commonly recognized, repeat-containing antigen of the pathogenic entamoeba histolytica hm-1:imss. k2p-1 was used to isolate 3 cdna clones (k2 clones); one k2p-1 related clone from the same pathogenic e. histolytica strain and 2 from the nonpathogenic e. histolytica strain saw-142. the nucleotide sequence and predicted amino acid sequence revealed a closely related family of transcripts differing main ... | 1993 | 8515783 |
| change of isoenzyme pattern during long-term polyxenic cultivation of entamoeba histolytica. | isoenzymes of phosphoglucomutase and hexokinase were repeatedly evaluated using starch gel electrophoresis in polyxenic cultures of entamoeba histolytica. in two out of 18 strains spontaneous changes of isoenzyme patterns were recorded. while originally they were categorized into virulent group of zymodemes, following isoenzyme analysis classified them as non-virulent. the relation between virulence and isoenzyme pattern is questionable. | 1993 | 8215112 |
| synthesis of some 4-oxo-delta 2-thiazolin-2-ylhydrazones as potential antiprotozoal agents. | several 4-oxo-delta 2-thiazolin-2-ylhydrazone derivatives 6 (6-1 - 6-19) derived from the cyclization reaction between the thiosemicarbazones 5 and homologous alpha-chloroacids were prepared. only compound 6-2 was found to be active against entamoeba histolytica and trichomonas vaginalis. | 1993 | 8216678 |
| the calcium binding protein of entamoeba histolytica: expression in escherichia coli and immunochemical characterization. | the gene encoding calcium binding protein (cabp) of entamoeba histolytica is single-copy and transcribes a single class of mrna. the coding region of the gene, amplified from a genomic clone of cabp by polymerase chain reaction, was cloned into the escherichia coli expression vector pet-3c. the expressed protein was 15 kd in size, which agreed with the predicted size from the open reading frame. the recombinant protein showed altered mobility in sodium dodecyl sulfate-polyacrylamide gel electrop ... | 1993 | 8220585 |
| purification of helicobacter pylori superoxide dismutase and cloning and sequencing of the gene. | the superoxide dismutase (sod) of helicobacter pylori, a pathogenic bacterium which colonizes the gastric mucosa, evoking a marked inflammatory response, was purified and characterized, and the n-terminal amino acid sequence was determined. the enzyme consists of two identical subunits each with an apparent molecular weight of 24,000. analysis of the primary structure and inhibition studies revealed that h. pylori possesses a typical procaryotic iron-containing enzyme. no other isoenzymes could ... | 1993 | 8225605 |
| influence of bacteria on electrophoretic proteinase patterns of entamoeba histolytica isolates. | gelatin sds- polyacrylamide gel electrophoresis was used to compare proteinase banding patterns under reducing conditions from whole cell lysates of four axenic and four xenic pathogenic strains of entamoeba histolytica. all strains shared major bands in the 34 and 66-68 kda regions, whereas only the axenic strains produced major bands at 26, 28, 30 and 45 kda. one axenic strain, nih 200, when reassociated with mixed bacterial flora, reverted to an electrophoretic banding pattern characteristic ... | 1993 | 8225772 |
| pharmacokinetics and amoebicidal activity of (+-)-(e)-3-(4- methylsulphinylstyryl)-1,2,4-oxadiazole (bti 2286e) and its sulphone metabolite (bti 2571e) in the golden hamster, mesocricetus auratus. | bti 2286e is a 1,2,4-oxadiazole with amoebicidal activity. three groups of golden hamsters received single doses of bti 2286e or its sulphone metabolite bti 2571e as either bti 2286e 60 mg/kg po or bti 2571e 60 mg/kg po or bti 2571e 60 mg/kg ip. blood samples were collected up to 8 h post-dose and plasma concentrations of bti 2286e and bti 2571e were assayed by hplc. bti 2286e was rapidly absorbed, extensively metabolized during first pass and rapidly eliminated with a plasma elimination half-li ... | 1993 | 8226401 |
| structural analysis of the light subunit of the entamoeba histolytica galactose-specific adherence lectin. | adherence of entamoeba histolytica trophozoites to colonic mucins and resistance to lysis by the membrane attack complex of complement are mediated by a galactose- and n-acetyl-d-galactosamine-specific cell-surface lectin. this lectin is a heterodimeric glycoprotein of heavy (170 kda) and light (35/31 kda) subunits. in this work, the amino acid sequence and membrane anchor of the light subunit were analyzed. the light subunit cdna encoded a protein with a calculated molecular mass of 32 kda cont ... | 1993 | 8226970 |
| degradation of human iga by entamoeba histolytica. | to determine whether the virulent enteric pathogen entamoeba histolytica degrades human iga molecules, serum and secretory iga was exposed to viable axenic trophozoites (strain hm1:imss), a parasite sonicate, and medium conditioned by incubation with live trophozoites. iga was completely degraded under all conditions, proteinase activity was maximal at a neutral ph, and there was a four- to eightfold enrichment of amebic iga proteolytic activity in a soluble fraction of amebic sonicate. degradat ... | 1993 | 8228372 |
| cerebral amoebiasis. | 1993 | 8230659 | |
| molecular cloning of a rac family protein kinase and identification of a serine/threonine protein kinase gene family of entamoeba histolytica. | eleven entamoeba histolytica protein-serine/threonine-kinase gene segments were identified using the polymerase chain reaction (pcr) and degenerate oligonucleotide primers to conserved amino acids in subdomains vi and viii of the catalytic domain of protein-serine/threonine kinases. these ameba gene segments were homologous to myosin light chain kinases, protein kinase c, phosphorylase b kinase, and kinases that regulate glucose repression in yeast and cell growth in mammalian cells. one of thes ... | 1993 | 8232409 |
| the cdna sequence of an abundant entamoeba histolytica 20-kilodalton protein containing four repetitive domains. | 1993 | 8232422 | |
| [amebic brain abscess. apropos of a case]. | we report a case of double primary amoebic brain abscess in a 22 year old man, with signs of intracranial hypertension and deficit. computed tomography found two hypodense lesions, not suggestive of pyogenic abscesses. aspiration of one of the two abscesses, revealed the organism, and the two abscesses regressed in response to medical treatment. the authors insist on the rarity of these abscesses, their gravity, and the difficulty of the diagnosis. | 1993 | 8239477 |
| design, synthesis and biological study of new antiparasitic spiroarsoranes. | thirty-eight new spiroarsoranes were synthesized after structure-activity relationship studies from the first series. these compounds were predicted to cross more easily the membrane of protozoae or the cuticle of nematodes and to reach their biological target with efficiency. the spiroarsoranes were evaluated for their antiparasitic properties, on helminths and protozoa models in regard of their parent arsonic acids. the following parasite models were used: entamoeba histolytica and trichomonas ... | 1993 | 8240448 |
| a variable dna region of entamoeba histolytica is expressed in several transcripts which differ in genetically related clones. | a highly variable dna region (ehvr1), isolated from entamoeba histolytica clone a, strain hm1:imss, is transcribed into several transcripts, which differ in genetically related clones. ehvr1 (3.5 kb) is composed of two contiguous fragments; one of these 1.9 kb long, at the 3' end, identified similar transcripts in clones a, l6 and c2 (all derived from strain hm1:imms), the other of 1.6 kb, at the 5' end, detected 0.5, 0.6 and 0.7 kb transcripts only in clone a. variability of the 1.6 kb fragment ... | 1993 | 8246880 |
| entamoeba histolytica: identification of a major surface antigen in liver abscess pus using a monoclonal antibody. | 1993 | 8249093 | |
| adherence of entamoeba histolytica to hydrophobic matrices: a simple method for measuring cell surface hydrophobicity. | 1993 | 8249095 | |
| entamoeba histolytica: a method for isolate identification. | the ability to identify individual isolates of entamoeba histolytica schaudinn 1903 (emend. walker 1911) is necessary before several important epidemiological questions can be answered. we have developed such a method based on our discovery of extensive polymorphism in two e. histolytica genes--the serine-rich antigen gene and the "strain specific gene"--each of which has an internal tandemly repeated structure. using the polymerase chain reaction we detected both size and restriction site polym ... | 1993 | 8253158 |
| entamoeba histolytica: an explanation for the reported conversion of "nonpathogenic" amebae to the "pathogenic" form. | the reported conversion of "nonpathogenic" entamoeba histolytica isolates to the "pathogenic" form during attempted axenization of the amebae is highly controversial. after failing to obtain conversions ourselves we concluded that the simplest explanation for the published observations would be contamination of nonpathogenic cultures with pathogenic amebae. to address this possibility we used a method based on analysis of stable dna polymorphisms that allows the positive identification of indivi ... | 1993 | 8253159 |
| [drinking water and parasites]. | entamoeba histolytica, giardia lamblia, cryptosporidium parvum, isospora belli, balantidium coli, and microsporidia spp. are cosmopolitan parasites. they often cause diarrheal diseases. the waterborn transmission of all these parasites is possible (41). surface water supplies used for drinking water are potential sources of contamination. giardia lamblia and cryptosporidium spp. have received great attention in industrialized countries during the last years because they are the etiological agent ... | 1993 | 8253478 |
| amebiasis and "nonpathogenic" intestinal protozoa. | infection with single or multiple species of intestinal protozoa is common in humans and can result in either asymptomatic colonization or symptoms of intestinal disease. entamoeba histolytica serves as a paradigm for invasive colonic protozoal infection. the key to diagnosis and treatment of amebiasis is knowledge of the epidemiologic risk factors and clinical manifestations, a rational approach to diagnosis, and an understanding of the sites of action and uses of anti-amebic drugs. this knowle ... | 1993 | 8254155 |
| [ameboma of the large intestine and rectum]. | amoeboma was diagnosed in three patients: a bengal seaman aged 40 and two dutch citizens who had lived in the tropics, a man aged 41 and a woman aged 56. the first-mentioned patient had a rectal tumour easily inspected by sigmoidoscopy; a biopsy sample contained entamoeba histolytica. the faeces contained no cysts. the two other patients had coecal amoebomas. they had been operated elsewhere on suspicion of appendicitis and had been treated with metronidazole, which had failed to eliminate the a ... | 1993 | 8255341 |
| schistosomiasis and intestinal parasites in rural villages in southwest nigeria: an indication for expanded programme on drug distribution and integrated control programme in nigeria. | a cross sectional survey of schistosomiasis, intestinal parasite infections and pattern of schistosomiasis transmission was undertaken in four rural villages around the erinle dam, nigeria. the prevalence of schistosoma haematobium ranged from 10% to 60%. 30% of infected school children excreted over 50 eggs/10 ml urine and high rates of haematuria, proteinuria, leucocyturia and nitrites in urine were observed in infected children and the villagers. prevalence of infection with s. mansoni ranged ... | 1993 | 8256092 |
| the proteases and pathogenicity of parasitic protozoa. | protozoan parasites are among the most prevalent pathogens worldwide. diseases like malaria, leishmaniasis, amebiasis, and trypanosomiasis affect hundreds of millions of people. recent advances in our understanding of the biochemistry and molecular biology of these organisms has focused attention on specific parasite molecules that are key to the parasite life cycle or the pathogenesis of the diseases they produce. one group of enzymes that plays myriad roles in these processes are the parasite- ... | 1993 | 8257117 |
| [fatal transmural colitis caused by entamoeba histolytica. differential diagnosis of chronic inflammatory bowel disease and colon cancer]. | perforation of the colon is a rare but frequently fatal complication of amoebiasis. we report a case of a 53 year-old male, with no history of travel abroad, who was admitted to hospital with haematochezia. a tumor of the rectum was diagnosed clinically. due to acute intestinal obstruction, laparotomy was performed, revealing multiple perforations of the large bowel and severe peritonitis leading to subtotal colectomy. the histological examinations revealed transmural amoebic colitis. the patien ... | 1993 | 8259618 |
| molecular and cell biology of opportunistic infections in aids. entamoeba histolytica. | 1993 | 8261181 | |
| differentiation of pathogenic entamoeba histolytica infections from nonpathogenic infections by detection of galactose-inhibitable adherence protein antigen in sera and feces. | we determined whether epitope-specific monoclonal antibodies to the galactose-inhibitable adherence protein (giap) of entamoeba histolytica could be used in an enzyme-linked immunosorbent assay (elisa) to detect antigen in serum and feces and differentiate between nonpathogenic zymodemes and the potentially invasive pathogenic organisms that require treatment. overall, 57% of subjects from cairo, egypt, with symptomatic intestinal amebiasis and 42% with asymptomatic infection possessed giap anti ... | 1993 | 8263165 |
| sequence analysis of genes encoding the light subunit of the entamoeba histolytica galactose-specific adhesin. | 1993 | 8264736 | |
| intestinal parasitic infections in campalagian district, south sulawesi, indonesia. | intestinal parasitic infections were surveyed in the inhabitants of 3 coastal and 2 inland villages of campalagian district, south sulawesi, indonesia, in july 1992. a total of 398 fecal samples were examined by using kato-katz thick smear, harada-mori culture and agar-plate culture techniques. protozoan cysts were examined by formalin ether concentration technique on 380 fecal samples. soil-transmitted helminth infections were highly prevalent with the overall positive rates as follows: ascaris ... | 1993 | 8266235 |
| unusual gene organization in the protozoan parasite entamoeba histolytica. | we have analyzed three independent genomic loci of the protozoan parasite entamoeba histolytica that contain coding regions for the iron-containing superoxide dismutase, the pore-forming peptide, and the galactose-inhibitable lectin. all of the three structural genes were found to be closely linked unidirectionally to other coding sequences. the intergenic regions did not exceed 1,350 nucleotides. nuclear run-on data demonstrated that at least the galactose-inhibitable lectin gene is transcribed ... | 1993 | 8274224 |
| entamoeba histolytica: inhibition of malic enzyme and alcohol dehydrogenase by (+/- )-, (+)-, and (-)- gossypol. | gossypol, a natural racemic mixture with action on nadp- and nad-oxidoreductases from diverse species, has been proposed as a possible antiamebic medication considering several of its pharmacological properties. in this study it was found that malic enzyme and alcohol dehydrogenase from entamoeba histolytica are strongly inhibited by (+/-)-gossypol, and both (+)- and (-)- enantiomers. the inhibition was of the noncompetitive type among their respective substrates in all cases. the (+/-), (+)-, ( ... | 1993 | 8274846 |
| pathogenicity of entamoeba histolytica isolates from shanghai, china. | 1993 | 8278345 | |
| the occurrence of human intestinal parasites in finland. | the survey of the occurrence of intestinal parasites presented here covers 726 hospital patients, 175 healthy adults and 212 children, and 976 healthy finnish holiday-makers prior to their trip abroad. two faecal samples from each individual were examined. further, 344 children were tested for enterobius vermicularis. the total prevalence of intestinal parasites in the hospital patients was 4.2%, and that of pathogenic parasites 2.9%. the prevalence rates in healthy subjects were 4.9% and 1.5%, ... | 1993 | 8284655 |
| a fluorescence-based quantitative adhesion assay to study interactions between entamoeba histolytica and human enterocytes. effect of proinflammatory cytokines. | in order to study the initial events during infection of target cells by the enteric pathogen entamoeba histolytica, we developed a quantitative adhesion assay based on the use of a human colonic cell line (caco-2) and biotinylated amoebae tagged with fluorescein. to prevent the strong and rapid lytic activity of entamoeba histolytica on colonic cells, which would otherwise impede the study of the primary adhesion steps, parasites were mildly fixed, biotinylated and labelled with streptavidin-fi ... | 1993 | 8288877 |
| production and characterization of monoclonal antibodies against a highly immunogenic fraction of entamoeba histolytica (nih:200) and their application in the detection of current amoebic infection. | six monoclonal antibodies (mabs) were produced against a highly immunogenic fraction derived by the chromatographic separation of the soluble preparation of axenic entamoeba histolytica (strain nih:200) trophozoites. isotype characterization of the six mabs revealed that four belonged to the igm class and one each to the igg1 and the igg2a subclasses. the immunoreactivity patterns and the specificity of the mabs with homologous and heterologous antigens were analyzed by the enzyme-linked immunot ... | 1993 | 8292992 |
| amoebic liver abscess--an unusual presentation. | 1993 | 8294371 | |
| correlation between membrane glycoprotein and detergent dissected membrane protein in the assessment of lmif: a report of 51 ala cases. | assessment of 51 amoebic liver abscess cases for leukocyte migration inhibition factor released using membrane glycoprotein and detergent dissected membrane protein (ddmp) of axenic entamoeba histolytica (nih:200). lymphokines release by t lymphocytes in response to purified amoebic membrane glycoprotein (pamg) against whole amoebic lysate (wal), dissect out protein against whole amoebic lysate and membrane glycoprotein against dissected protein was tested by leukocyte migration inhibition test ... | 1993 | 8300172 |
| a comparative study of different isolated fractions of entamoeba histolytica in amoebic liver abscess. | fifty six patients with amoebic liver ubscess were assessed for antibody production and lymphokine release using different antigenic fractions such as sephadex g-200 eluted fraction-i (f-i), shed of bile salt treated amoebae (sbsta), amoebic membrane glycoprotein (amg), amoebic rna and whole amoebic lysate (wal). antibody production in response to wal, f-i, sbsta, amg and rna was assessed by indirect haemagglutination assay. five and 53 fold increased titres of iha was observed with f-i and sbst ... | 1993 | 8171729 |
| axenic cultivation of entamoeba histolytica from liver abscess and its zymodeme. | a local strain of entamoeba histolytica, the hth-56: mutm from a human liver abscess was successfully axenized. the culture was initially established monoxenically in diamond's tyi-s-33 medium in the presence of crithidia luciliae and maintained at 34 +/- 0.5 degrees c. after 5 passages it was adapted to axenic cultivation by addition of 0.02% bacto agar in diamond's tyi-s-33 medium in place of crithidia. subcultures or replacement with fresh complete media were done twice or thrice for 7 days, ... | 1993 | 8160057 |
| immunological similarity between the 170 kd amoebic adherence glycoprotein and human beta 2 integrins. | amoebiasis is an important cause of morbidity and mortality in developing countries. virulence of entamoeba histolytica depends on the trophozoite's being able to bind to colonic epithelium; binding is mediated by the 170 kd subunit of a 260 kd lectin. we found, by microcytofluorometry and western blotting studies, that this 170 kd subunit protein shares an epitope with human beta 2 integrins--a subfamily of cell-surface glycoproteins expressed exclusively on leucocytes and involved in many adhe ... | 1993 | 7678048 |
| structural analysis and demonstration of the 29 kda antigen of pathogenic entamoeba histolytica as the major accessible free thiol-containing surface protein. | the 29 kda protein of pathogenic entamoeba histolytica is a cysteine-rich surface antigen which we recently characterized by cdna sequencing and by using monoclonal antibodies which differentiated between pathogenic and non-pathogenic clinical isolates. to determine the structure and biochemical attributes of this protein, a repertoire of immunological techniques using monoclonal antibodies, and radiolabelling were employed. we demonstrated that the 29 kda protein forms a 60 kda dimer and a high ... | 1993 | 7682280 |
| neutralizing monoclonal antibody epitopes of the entamoeba histolytica galactose adhesin map to the cysteine-rich extracellular domain of the 170-kilodalton subunit. | entamoeba histolytica adheres to human colonic mucins and colonic epithelial cells via a galactose-binding adhesin. the adhesin is a heterodimeric glycoprotein composed of 170- and 35-kda subunits. fragments of the hgl1 gene encoding the 170-kda subunit were expressed as recombinant fusion proteins in escherichia coli and reacted with anti-adhesin monoclonal antibodies (mabs) or pooled human immune sera. the mabs tested recognize seven distinct epitopes on the 170-kda subunit and have distinct e ... | 1993 | 7682994 |
| [bacterial, viral and parasitic etiologies of acute infantile diarrhea in a rural senegal]. | we have studied the incidence of parasites, viruses and bacteria associated with acute infantile diarrhea in rural senegal. among parasitoses, cryptosporidium parvum (7.7%) is the most frequent, followed by entamoeba histolytica (5.1%). the first bacteriological etiologic agent is escherichia coli (20.9%). rotavirus are also frequently isolated (14.7%). prevalence of pathogens is higher: among children over 6 months and under two years old, among children either with mixed feeding or weaned, dur ... | 1993 | 7758380 |
| [the infectious etiology of acute diarrheal diseases in the republic of cuba, 1991]. | this study was carried out in 1991 to learn the behavior of enteropathogenic agents causing acute diarrheal diseases in cuba. 30 children, admitted in hospitals or seen in outpatient services for acute diarrheal diseases, were selected in each province taking into account that they had not received antibiotic or chemotherapy treatments in the previous 72 hours. feces samples were taken from all patients for virological, bacteriological, and parasitological studies, and results were sent to the p ... | 1993 | 7984819 |
| diagnostic methods for intestinal parasites in southern iraq with reference to strongyloides stercoralis. | three hundred and thirty-two stool samples were examined for the presence of intestinal parasites including strongyloides stercoralis. each sample was processed and examined by direct smear, formalin-ether and harada and mori culture methods. nine parasites were recovered from patients attending basrah teaching hospital, southern iraq during 1989. the prevalence rate of infection was 64.2%. it was higher in rural (74.2%) than in urban (57.5%) region (p < 0.01). sex distribution was 120 (36.1%) m ... | 1993 | 7939941 |
| [analysis of pathogenicity of entamoeba histolytica by polymerase chain reaction]. | dna from five isolates of entamoeba histolytica were examined for their pathogenicity by polymerase chain reaction. three isolates sh-3,sh-6,sh-8 were isolated from patients with acute amoebic dysentery, whereas sh-5 and sh-7 were isolated from asymptomatic cyst passers. gel electrophoresis of pcr products showed that primers p11, p12 for pathogenic strains could amplify genomic dna extracted from sh-8, and primers p13, p14 for nonpathogenic strains could amplify genomic dna extracted from sh-3, ... | 1993 | 8168237 |
| [research of antiparasitic agents: 1(nitrophenyl)-3-(methyl-3-indolyl)-prop-2-ene-1-ones (nitroindolylchalcones)]. | the synthesis and the study of the antiparasitic properties of various 1-(nitro-phényl)-3-(méthyl-3-indolyl)-prop-2-ene-1-ones (nitroindolylchalcones) show interesting antiprotozoal activities on e. histolytica and t. vaginalis. no significative activity appears in vivo with animals on helminthes. | 1993 | 8154794 |
| endometrial amoebiasis. | a case of endometrial infection by entamoeba histolytica is described in an elderly lady who presented with profuse vaginal discharge and was clinically misdiagnosed as endometrial carcinoma. | 1993 | 8157142 |
| crude or recombinant proteins applied to latex agglutination, complement fixation and enzyme-linked immunosorbent assays for the serodiagnosis of invasive amebiasis. | three different agglutination tests were developed for the detection of serum antibodies against entamoeba histolytica. these tests are based on carboxylated polystyrene beads loaded either with a purified recombinant e. histolytica protein, designated receh-p1, or a soluble fraction, or a membrane fraction (m-la) both prepared from e. histolytica trophozoites. the three agglutination tests were compared with an enzyme-linked immunosorbent assay and a complement fixation test based on crude solu ... | 1993 | 8134767 |
| [prevalence of enteropathogens in children with liquid diarrhea]. | between january 1 and october 31, 1991 148 samples of feces corresponding to the same number of children with acute diarrhea, aged 0-2 years, admitted to hospital o'horan of the secretariat of health in the city of merida, yucatan, mexico, were studied. stools from 105 (70.9%) patients were described as watery diarrhea; from these samples at least one enteric pathogen was detected in 49 (46.6%): a single pathogen was isolated in 37 (35.2%), and mixed infections were observed in 12 (11.4%). we is ... | 1993 | 8066329 |
| [evaluation of 3 tests applied in the immunological diagnosis of hepatic amebiasis in gabon]. | the diagnosis of hepatic amoebiasis is not always easy. in case of suspicion of hepatic amoebiasis in an endemic area, the patient is immediately treated with a 5-nitroimidazole. immunological diagnosis is indispensable. after a comparative study of bla, iha and ifa reactions, the bla test has shown to be well adapted to the context of a country with insufficient sanitary infrastructure. the bla test is easily performed and enables rapid detection of antibodies. the result obtained in about 5 mi ... | 1993 | 8129470 |
| differential effects of the (+)- and (-)-gossypol enantiomers upon entamoeba histolytica axenic cultures. | the in-vitro anti-amoebic effects of (+/-)-, (+)-, (-)-gossypol and emetine were tested against axenic trophozoites from five entamoeba histolytica strains. the (-)-isomer was more active than the racemate and the (+)-isomer. these results indicate that the gossypol anti-amoebic activity is mainly due to its content of (-)-gossypol in all strains tested. | 1993 | 8095532 |
| [intestinal parasite infections in rural students of chiloé archipelago, x region, chile]. | in order to contribute to the knowledge of intestinal parasite infection a survey was performed in 462 primary school children from eight rural localities sited in two islands of the chiloe archipelago. stool samples and perianal scotch tape scrapings for intestinal protozoa and helminths were microscopically examined. the prevalence rates of the main species found were: ascaris lumbricoides 43.3%, trichuris trichiura 26.8%, enterobius vernicularis 20.1%, entamoeba histolytica 5.6%, giardia inte ... | 1993 | 8110371 |
| a glyceraldehyde-3-phosphate dehydrogenase with eubacterial features in the amitochondriate eukaryote, trichomonas vaginalis. | glyceraldehyde-3-phosphate dehydrogenase (gapdh), localized in the cytosol of trichomonas vaginalis, was partially purified. the enzyme is specific for nad+ and is similar in most of its catalytic properties to glycolytic gapdhs from other organisms. its sensitivity to koningic acid is similar to levels observed in gapdhs from eubacteria and two orders of magnitude lower than those observed for eukaryotic gapdhs. the complete amino acid sequence of t. vaginalis gapdh was derived from the n-termi ... | 1993 | 8114116 |
| analysis of a polymorphic locus present upstream of rdna transcription units in the extrachromosomal circle of entamoeba histolytica. | 1993 | 8114815 | |
| molecular cloning of the gene for a novel abc superfamily transporter of entamoeba histolytica. | 1993 | 8114816 | |
| primary structure of the pyruvate phosphate dikinase in entamoeba histolytica. | 1993 | 8114821 | |
| analysis of the gene family encoding the entamoeba histolytica galactose-specific adhesin 170-kda subunit. | the 170-kda or heavy subunit of the galactose binding adhesin of entamoeba histolytica is seminal in target cell binding and lysis. to determine the existence and complexity of the 170-kda subunit gene family, hgl, an amebic genomic library in lambda phage was hybridized with dna fragments from the 5' or 3' ends of hgl1. termini from three distinct heavy subunit genes were identified including hgl1, hgl2, and a third, unreported gene designated hgl3. the open reading frame of hgl3 was sequenced ... | 1993 | 8114826 |
| intracellular digestion of human erythrocytes by entamoeba histolytica: a kinetic study in vitro. | the objective of this investigation was to quantitatively evaluate, by using light and transmission electron microscopy, the intracellular digestion of human erythrocytes (he), ingested by entamoeba histolytica trophozoites in vitro. amebas fed 10 min with he were postincubated 3-12 h at 36.5 degrees c without he, and fixed with glutaraldehyde. after staining by the benzidine reaction, he per ameba, and percent of amebas containing at least one he were determined by light microscopy. trophozoite ... | 1993 | 8118158 |
| zymodeme alteration of entamoeba histolytica isolates under varying conditions. | cysts of entamoeba histolytica obtained from 2 asymptomatic subjects were cloned in vitro and the isoenzyme patterns and virulence of the cultures derived from them were determined. incubation in diamond's tyi-s-33 medium with crithidia sp. for 80 d resulted in a change from zymodeme iii to zymodeme ii, with consistent virulence. axenization by replacing crithidia sp. with crithidial lysate had no further effect on the zymodeme but the virulence of the amoebae was attenuated. a reversal to zymod ... | 1993 | 8080488 |
| extra-intestinal amebiasis: clinical presentation in a non-endemic setting. | 37/38 patients with reciprocal titers > or = 512 against entamoeba histolytica in denmark over a 5-year period were evaluated retrospectively in order to establish the clinical profile of extra-intestinal amebiasis in a non-endemic area. 24 of these had extra-intestinal amebiasis, all presenting 1 or more amebic liver abscesses on ultrasonography. fever was the most common finding, present in 91% of the cases. the most striking observation was the lack of both abdominal pain and tenderness in 22 ... | 1993 | 8052815 |
| enzyme location and metabolism in entamoeba histolytica. | 1993 | 8032076 | |
| an attempt at reversibility and increase of the virulence of axenic strains of entamoeba histolytica. | in this study we have tried to verify whether the interaction "in vitro" with bacteria or small pieces of normal hamster liver would modify the pathogenic behavior of axenic strains of e. histolytica: avirulent ones (icb-32 and icb-rps), of attenuated virulence (icb-csp and hm1) and of mean virulence (icb-462). every attempt to render virulent, recover or increase the virulence of axenic strains of e. histolytica has failed. | 1993 | 7997753 |
| entamoeba histolytica zymodemes in children of osasco, são paulo. | 1993 | 7997764 | |
| recent trends in the diagnosis of amoebiasis. | 1993 | 8005981 | |
| ultrastructural arguments for cytoplasmic engulfment phenomenon in nucleus of entamoeba histolytica. | the electron optic study of a pathogenic e. histolytica strain revealed particular aspects concerning the shape, structure and distribution in the nuclear space of the intranuclear structures known as button-like bodies. these aspects are ultrastructural arguments useful in interpreting the intranuclear inclusions as sections through the cytoplasmic material sequestered in the nuclear space by karyophagocytosis. | 1993 | 8173125 |
| activity of a new oxadiazole compound, against experimental infections with entamoeba histolytica and giardia lamblia in animal models. | bti 2286e(+/-)-e-3-(4-methylsulphinylstyryl)-1,2,4-oxadiazole has demonstrated potent amoebicidal activity in a single-dose treatment against entamoeba histolytica infection in the livers of golden hamsters and the caeca of mice, hamsters and rats. it has intra luminal activity against entamoeba criceti, a natural infection in golden hamster, and anti-giardial activity against giardia lamblia infection in suckling mice. bti 2286e is more potent than metronidazole in extra intestinal, intra lumin ... | 1993 | 8561524 |
| molecular mechanisms of invasion by entamoeba histolytica. | amebiasis is the third leading cause of death due to parasitic disease. adherence to and contact-dependent killing of host cells requires the galactose-inhibitable lectin, a heterodimeric glycoprotein composed of heavy and light subunits. the cysteine-rich extracellular domain of the heavy subunit has identity with beta 1 integrins, complement receptor cd59, and complement components c8 and c9; the light subunit sequence is unlike any other sequenced protein. monoclonal antibodies to the cystein ... | 1993 | 7504959 |
| prevalence of gastrointestinal infection among international travellers returning to canada. | to describe the prevalence of parasitic and bacterial gastrointestinal infection (excluding enterotoxigenic escherichia coli) among international travellers attending the international travel clinic at the university of calgary. | 1993 | 22346439 |
| recognition of entamoeba histolytica lipophosphoglycan by a strain-specific monoclonal antibody and human immune sera. | western blot analysis showed that the monoclonal antibody 2d7.10 recognized lipophosphoglycan (lpg) from entamoeba histolytica hm-1:imss. sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) pattern of [3h]galactose-labeled lpg and western blot analysis of total lysate of e. histolytica with 2d7.10 revealed patterns similar to that of lpg with 2d7.10. this antibody could also immunoprecipitate purified lpg from the strain hm-1:imss after biosynthetically labeling with [3h]galacto ... | 1992 | 1283004 |
| susceptibility of an emetine-resistant mutant of entamoeba histolytica to multiple drugs and to channel blockers. | previously a cloned emetine-resistant mutant of the protozoal parasite entamoeba histolytica was shown to overexpress a gene for an ameba homolog of the mammalian p-glycoprotein, a plasma membrane pump that removes hydrophobic drugs from multidrug-resistant tumor cells. three sets of experiments were performed to better characterize the multidrug-resistant phenotype of the emetine-resistant amebae. first, the emetine resistance of the mutant amebae was reversed by concentrations of calcium and s ... | 1992 | 1283295 |
| membrane perforation by entamoeba histolytica: structural implications derived from the sequence of the pore-forming peptide. | the pore-forming peptide amebapore is considered crucial for the cytolytic activity of e. histolytica. isolation and subsequent molecular cloning of the peptide allowed predictions as to its secondary structure, most of which were confirmed by experimental data. here, a computer-aided approach is applied to identify, within the peptide, highly amphipathic helical segments predicted to interact with biological membranes. two putative alpha-helices fulfill the criteria of membrane-seeking domains ... | 1992 | 1285083 |
| cloning and partial characterization of an antigen detected on membrane surfaces of non-pathogenic strains of entamoeba histolytica. | a novel and unique mab (318-28) which specifically interacts with a 60 kda antigen that is found only on the surfaces of non-pathogenic (np) strains of e. histolytica has been recently characterized. the antigen appears to be present also on (np) cyst forms of amebas but was not detected on any of the various (p)-strains tested. it was also not found on other entamoeba species such as moshkovskii, laredo, huff, coli, gingivalis, or invadens. clinical trails for the differentiation of (p) and (np ... | 1992 | 1285084 |
| sequence and structure of the galactose adherence lectin of entamoeba histolytica. | 1992 | 1285085 | |
| a rapid colorimetric assay with the tetrazolium salt mtt and phenazine methosulfate (pms) for viability of entamoeba histolytica. | a rapid colorimetric assay for viability of e. histolytica trophozoites using the tetrazolium salt mtt and phenazine methosulfate (pms) was developed. the mtt is normally reduced by dehydrogenases of viable cells and transformed to formazan, a colored compound; the reaction is catalyzed by pms. trophozoites of e. histolytica were incubated in the presence of mtt and pms during 15, 30, 45, 60 and 75 min, at 37 degrees c. trophozoites killed by heat or cold were used as controls. the formazan prod ... | 1992 | 1285086 |
| structural organization of chromatin during the cell cycle of entamoeba histolytica trophozoites. | the nuclear division of e. histolytica trophozoites was analyzed by using specific stains for dna, with the aim to define the sequential changes of chromatin during its life cycle. furthermore, we characterized the internal structural arrangements of microtubules in the microtubular organizing center (mtoc) and determined the number of chromosomes and its association with the spindle. the mtoc is formed by multiple microtubule-nucleating centers, that are involved in the displacement of dna duri ... | 1992 | 1285087 |
| human infections of entamoeba chattoni masquerade as entamoeba histolytica. | 1992 | 1287923 | |
| energy metabolism of ancestral eukaryotes: a hypothesis based on the biochemistry of amitochondriate parasitic protists. | parasitic amitochondriate protists, representatives of early branches of eukaryote evolution, differ considerably in their central, energy metabolism from mitochondrion-bearing cells. these differences are: significant metabolic functions of inorganic pyrophosphate, major role of iron-sulfur proteins in key metabolic steps and in hydrogenosome-bearing organisms the disposal of electrons by h2 formation. cytochrome-mediated electron transport and electron transport-linked phosphorylation are abse ... | 1992 | 1292665 |
| pilot studies on the occurrence of some infectious diseases in two different areas in south yemen (aden). part i. parasitology. | two pilot studies on malaria, leishmaniasis, schistosomiasis and intestinal parasites were carried out in 104 children 6-15 years old from villages in the lowland and highland areas of south yemen in november 1988. some of the results presented are in the order lowland and highland. the occurrence of malaria parasites in blood smears was 6.7% and 3.8%. p. falciparum was detected only. the antimalarial ifat antibodies were proved in 66.7% and 11.5% respectively. ifat antibodies against leishmania ... | 1992 | 1293209 |
| isolation of entamoeba histolytica from arthritic knee joint. | amoebae were isolated from an arthritic knee of a male patient. the organisms grew in egg yolk infusion medium, and in diamond's biosate iron-serum-33 medium, which contained trypanosoma cruzi, and were identified microscopically as entamoeba histolytica. furthermore, amoebae-like organisms in aspirate and cultures were immunocytochemically identified using monoclonal antibodies against e. histolytica. on the basis of the morphologic and immunologic observations, it was concluded that the knee l ... | 1992 | 1295146 |
| [the living and health conditions of palestinian children]. | young people constitute 46% of the population in the west bank of israel and 48% in the gaza strip with a rate of growth of 3.1% and 3.4%, respectively. their living conditions are characterized by lack of sanitation, shortage of water (especially in comparison to israel proper), unemployment, curfews and closing of schools during tense periods by the military authorities as collective punishment for incidents. since 1967, israel has controlled and administered the health care service in these ... | 1992 | 1296273 |
| inhibition of target cell adhesion by monoclonal antibody to the 66 kd surface antigen of entamoeba histolytica. | the adherence of entamoeba histolytica trophozoites to target cells was studied using a monoclonal antibody to a major surface antigen of 66 kd. preincubation of trophozoites with monoclonal antibody decreased their ability to adhere to and engulf erythrocytes and to destroy chinese hamster ovary cells. the monoclonal antibody was specific for the 66 kd antigen, which is possibly a major participant in the adhesion that precedes phagocytosis and cytopathic effects. | 1992 | 1296611 |
| partial purification of acid phosphomonoesterase in axenically grown entamoeba histolytica nih-200. | entamoeba histolytica possesses significant acid phosphatase activity as compared to alkaline phosphatase activity. the acid phosphatase activity in the amoebic cells eluted at higher saline concentration as three distinct peaks at 200, 300 and 400 mm sodium chloride. | 1992 | 1296952 |
| colonic immunity in patients and amoebic liver abscess. | secretory immunoglobulin a (s-iga), coproantibody titre (antiamoebic) and iga, igg, igm immunocytes in rectal mucosa were studied in 13 patients with amoebic liver abscess (ala) prior to and 4-6 weeks after completion of antiamoebic therapy. ten asymptomatic entamoeba histolytica cyst passers and 17 healthy age and sex matched volunteers served as controls. fecal s-iga levels and counts of iga bearing immunocytes in mucosa were significantly higher in patients with ala and cyst passers as compar ... | 1992 | 1296954 |