Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| dominant cell wall proteins of mycobacterium leprae recognized by monoclonal antibodies. | a cell wall fraction of mycobacterium leprae has enhanced potency in activating immune t cells. by using a panel of monoclonal antibodies (moab), the dominant immunogen in this preparation was shown to be a complex of proteins of apparent molecular weight (mr) 65 to 50 kd with a major antigen of 65 kd. antigen capture assays supported the results of immunoblots and elisa that this protein was concentrated in the cell wall. by varying the moab used as capture or tracer antibody, one of the three ... | 1987 | 3304738 |
| exochelin-mediated iron acquisition by the leprosy bacillus, mycobacterium leprae. | exochelins, water-soluble siderophores of mycobacteria, were isolated and partially purified from culture filtrates of iron-deficiently grown cultures of mycobacterium neoaurum nctc 10439 and an armadillo-derived mycobacterium (adm 8563). two biologically active fractions mediating iron uptake were isolated from each bacterium which not only were able to transport iron into the producing organism but also into suspensions of mycobacterium leprae isolated from armadillo liver. the rate of exochel ... | 1987 | 3309144 |
| detection of trehalose monomycolate in mycobacterium leprae grown in armadillo tissues. | trehalose-6-monomycolate (tmm) was isolated from the lipids of armadillo-derived mycobacterium leprae. only meagre amounts of this glycolipid were recovered, but its structure was unequivocally established. only alpha-mycolates were detected in the tmm by 252cf plasma desorption mass spectrometry. electron impact mass spectrometry showed the alpha branch to be principally c20. trehalose dimycolate (cord factor) was not detectable. since we have also found tmm in m. lepraemurium and in every myco ... | 1987 | 3309145 |
| peptidoglycan and arabinogalactan of mycobacterium leprae. | the arabinogalactan and peptidoglycan of armadillo-grown mycobacterium leprae were examined. within the limits defined by the small amount of material available, the resemblance of these polymers to those of other mycobacteria was confirmed. the polymers were linked by a highly acid-labile bond and the arabinogalactan was itself acid-labile; free arabinose and a variety of oligosaccharides containing both arabinose and galactose, as well as polysaccharide and peptidoglycan, were released by dilu ... | 1987 | 3309180 |
| unexpected findings amongst the skin test responses to mycobacteria of bcg vaccinated kuwaiti school children. | a multiple skin test survey was carried out in kuwait on 1200 school children aged 8-11 years, and on 1228 children aged 12-16 years. with only 15 exceptions, all these children had received vaccination with japanese bcg just before they started school, 5 years and 9 years earlier respectively. tuberculin positivity was almost 90% in both groups, with a mean response size of 8.7 mm. this was associated with remarkably high responsiveness to many of the other mycobacterial species investigated. s ... | 1987 | 3310346 |
| experimental leprosy in african green monkeys (cercopithecus aethiops): a model for polyneuritic leprosy. | three african green monkeys (cercopithecus aethiops) were inoculated intravenously and intracutaneously with mycobacterium leprae derived from a naturally infected mangabey monkey. all developed cutaneous lesions at inoculation sites. one developed disseminated cutaneous lesions, while the cutaneous lesions in the other two regressed and eventually disappeared. the animals were examined at necropsy five years after inoculation. all three had active leprosy infection in peripheral nerves with ext ... | 1987 | 3310689 |
| human t cell proliferative responses to particulate microbial antigens are supported by populations enriched in dendritic cells. | the efficacy of dendritic cells in antigen presentation was studied in eight healthy subjects using a lymphoproliferation assay. both particulate (mycobacterium leprae, h37ra) and soluble (ppd, tetanus toxoid) bacterial antigens were used as stimulants over a concentration range of accessory cells (monocytes (mo) and dendritic cells (dc)) varying from 10 to 0.1% in co-cultures using t-enriched cells. in general, co-cultures with t + mo and t + dc at all concentrations of accessory cells showed s ... | 1987 | 3311498 |
| most mycobacterium leprae carbohydrate-reactive monoclonal antibodies are directed to lipoarabinomannan. | each of more than 30 monoclonal antibodies that had been raised against mycobacterium leprae and previously classified as reactive with carbohydrate was shown to be directed against lipoarabinomannan, a prominent, highly pervasive, myo-inositol-phosphate-containing, cross-reactive antigen within the leprosy bacillus. some of the antibodies preferentially bound to the lipopolysaccharide of m. leprae rather than to that of mycobacterium tuberculosis, suggesting the presence of distinguishing struc ... | 1987 | 3312018 |
| the potential role of dna technology in leprosy. | 1987 | 3312894 | |
| primary dapsone resistance in bamako and chingleput: final report; thelep. subcommittee on clinical trials of the chemotherapy of leprosy (thelep) scientific working group of the undp/world bank/who special programme for research and training in tropical diseases. | 1987 | 3312895 | |
| studies of reactivity of some sri lankan population groups to antigens of mycobacterium leprae. ii. reactivity to a soluble protein antigen. | 1987 | 3312896 | |
| immunological effects of lepromin testing in sri lankan population groups. ii. effect on reactivity to a soluble protein antigen of mycobacterium leprae. | 1987 | 3312897 | |
| serodiagnosis of leprosy with pgl-1 using elisa technique. | 1987 | 3315988 | |
| mycobacterium leprae surface components intervene in the early phagosome-lysosome fusion inhibition event. | bone marrow-derived cultured macrophages were infected with mycobacterium leprae. the bacteria were either used as freshly isolated organisms or incubated with m. leprae antiserum (1:5) for 30 min prior to phagocytosis. immediately after inoculation (1 to 4 h) and at 1 to 8 days later, macrophages were stained for acid phosphatase activity to assess fusions between phagosomes and lysosomes. inhibition of fusions was essentially apparent as an early event, which was partially reversed by antiseru ... | 1987 | 3316028 |
| autoaggressive hanseniasis. | i have given the name autoaggressive hanseniasis to a syndrome with immunopathologic and clinical pictures resembling autoaggressive systemic diseases observed in some cases of lepromatous and borderline hanseniasis. it is probably caused by b cell stimulation by antigenic complex of mycobacterium leprae plus autologous tissue, along with a dysfunction of the t-suppressor lymphocytes. | 1987 | 3323269 |
| antibodies to a synthetic analog of phenolic glycolipid-i of mycobacterium leprae in healthy household contacts of patients with leprosy. | fifty-four household contacts of lepromatous patients, 39 household contacts of tuberculoid patients, and 99 control persons were examined with an enzyme-linked immunosorbent assay for their antibody responses to phenolic glycolipid-i (pgl-i) of mycobacterium leprae using a synthetic analog (pgl-isa) with the same terminal sugar epitope, namely, o-(3, 6-di-o-methyl-beta-d-glucopyranosyl)-(1----4)-o-(alpha-l-rhamnopyranosyl )-(1----9)-oxynonanoyl-bsa. this study was conducted in the gurage area o ... | 1987 | 3323365 |
| seroepidemiological study on 724 household contacts of leprosy patients in french polynesia using disaccharide-octyl-bsa as antigen. | a seroepidemiological surveillance of a contact population was started in 1984 in french polynesia. the elisa test was used to measure igm anti-nd-o-bsa in the sera. specific antibody levels were higher in healthy polynesians than in normal individuals living in a nonendemic country. the positive threshold of the reaction was fixed according to this background activity in healthy polynesians. under these conditions, 100% of the multibacillary patients were detected as seropositive as compared to ... | 1987 | 3323366 |
| antibodies to phenolic glycolipid-i during long-term therapy: serial measurements in individual patients. | levels of igm antibody to phenolic glycolipid-i (pgl-i) were measured in serum specimens collected over the initial 5 or more years of therapy from 11 leprosy patients. all three patients with paucibacillary disease had undetectable levels of antibody throughout their treatment. the eight patients with multibacillary disease had initially elevated levels which fell quite rapidly with treatment, reaching levels of 10% to 30% of their initial pre-treatment level after 5 years of therapy. the singl ... | 1987 | 3323367 |
| effects of icrc antileprosy vaccine in healthy subjects. | long-term effects of the administration of the icrc antileprosy vaccine in healthy subjects have been investigated both in household contacts of leprosy patients and noncontacts in a general population. each volunteer received a dose of vaccine containing either 0.5 x 10(9) or 1.7 x 10(7) bacilli intradermally. the vaccine induces a dose-dependent lepromin conversion in negative subjects at 8 weeks after vaccination. one year later, the conversion rates are more than 90% in both high- and low-do ... | 1987 | 3323368 |
| primary and secondary dapsone resistance of m. leprae in martinique, guadeloupe, new caledonia, tahiti, senegal, and paris between 1980 and 1985. | primary and secondary dapsone resistance were studied among lepromatous patients living in martinique, guadeloupe, new caledonia, tahiti, senegal, and paris. four hundred fifteen biopsies were taken from clinically active and bacteriologically positive (bacterial index greater than 2) patients in the 6-year period of 1980-1985. among these, 280 biopsies that contained 5 x 10(4) acid-fast bacilli per ml with a morphological index of at least 0.10 were inoculated into the mouse foot pad, and 229 h ... | 1987 | 3323369 |
| appearance of a methoxy mycolate-like component by the acid methanolysis of mycobacterium leprae. | it has been reported that mycobacterium leprae contains two types of mycolic acid, namely, alpha- and keto-mycolic acids, thus it is taxonomically similar to m. bovis bcg. however, there was some controversy about the presence of methoxy mycolic acid which was observed in small amounts only in the case of experimentally infected (w45) armadillo-derived m. leprae. to investigate this fact, mycolic acids were extracted from the cell-wall structure of m. leprae and characterized using chromatograph ... | 1987 | 3323370 |
| serological reactivity and early detection of leprosy among contacts of lepromatous patients in cebu, the philippines. | 1987 | 3323372 | |
| immunodiagnostic tests for leprosy; a need for standards. | 1987 | 3323373 | |
| dapsone susceptibility of m. leprae before and after 1977. | 1987 | 3323374 | |
| [hansen i. considerations on its development]. | the causes of the existence and importance of the i form are discussed, as were as its characteristics and the advantage of using the letter i to refer to this group. it is called initial because it is the way the disease first appears and due to its epidemiological value. the method used in the research carried out in the department of dermatology of the pic on 444 patients with i form in the register of patients in the pic is 16%. the average age ranged from 30 to 39 years old. most of the pat ... | 1987 | 3323709 |
| [delayed cutaneous response to different antigens in household contacts of leprosy patients and controls]. | the delayed type hypersensitivity (dth) response to ppd, candidin and histoplasmin, and mitsuda reaction (mr) in household contacts (hc) of multibacillary (hcm) and paucibacillary (hcp) leprosy patients, and non exposed controls was studied. intradermal tests with 0.1 ml. of each antigen were performed and read after 48 hours. late nodular reaction to ml (mr) was evaluated at the 21st day post-challenge. as we had formerly demonstrated, mr was depressed in hcm. interestingly, this reaction was a ... | 1987 | 3323710 |
| persisting mycobacterium leprae among thelep trial patients in bamako and chingleput. subcommittee on clinical trials of the chemotherapy of leprosy (thelep) scientific working group of the undp/world bank/who special programme for research and training in tropical diseases. | 1987 | 3323750 | |
| studies of reactivity of some sri lankan population groups to antigens of mycobacterium leprae. iii. the post-lepromin test scar in healthy populations in sri lanka. | 1987 | 3323752 | |
| the killing of mycobacterium leprae in mice by various dietary concentrations of clofazimine and ethionamide. | 1987 | 3323753 | |
| endothelial cell bacillation in lepromatous leprosy: a case report. | 1987 | 3323754 | |
| multiplication of mycobacterium leprae in normal mice. | 1987 | 3325572 | |
| mouse breeding and husbandry. | 1987 | 3325573 | |
| application of the mouse foot-pad technique in immunologically normal mice in support of clinical drug trials, and a review of earlier clinical drug trials in lepromatous leprosy. | 1987 | 3325574 | |
| drug susceptibility testing of mycobacterium leprae. | 1987 | 3325575 | |
| screening of drugs for activity against mycobacterium leprae. | 1987 | 3325576 | |
| the normal mouse in experimental chemotherapy. | 1987 | 3325577 | |
| application of the thymectomized-irradiated mouse to the detection of persisting mycobacterium leprae. | 1987 | 3325579 | |
| the thelep controlled clinical drug trials. subcommittee on clinical trials of the chemotherapy of leprosy (thelep). scientific working group of the undp/world bank/who special programme for research and training in tropical diseases. | 1987 | 3325580 | |
| detection of persisting mycobacterium leprae by inoculation of the neonatally thymectomized rat. | 1987 | 3325581 | |
| the neonatally thymectomized rat as a model of the lepromatous patient. | 1987 | 3325582 | |
| multiplication of mycobacterium leprae in the nude mouse, and some applications of nude mice to experimental leprosy. | two aspects of the immune deficiency of nude mice make these animals particularly useful tools for leprosy research. nude mice are capable of supporting multiplication of m. leprae to levels approaching 10(10) per g in peripheral body tissues. in addition, nude mice may be inoculated with greater than 10(4) (in fact, with as many as 10(8) organisms per foot pad, without provoking an immune response that prevents multiplication of the organisms. thus, the nude mouse should be particularly suitabl ... | 1987 | 3325583 |
| infection of other experimental animals with mycobacterium leprae. | 1987 | 3325584 | |
| analysis of dimycocerosates of glycosylphenolphthiocerols in the identification of some clinically significant mycobacteria. | extracts of representative mycobacteria were examined by thin-layer chromatography for glycosylphenolphthiocerol dimycocerosates. the glycolipid typical of mycobacterium bovis was also found in mycobacterium africanum and mycobacterium microti, but it was absent in mycobacterium bovis an 5. mycobacterium gastri strains contained a glycolipid which was chromatographically similar to that in mycobacterium kansasii. representatives of mycobacterium marinum produced a distinct glycolipid type, and o ... | 1987 | 3326746 |
| recombinant dna technology and its application to leprosy research. | 1987 | 3326902 | |
| mycobacterium leprae can penetrate nasal mucosa. | 1987 | 3326904 | |
| on the "culture medium for cultivation of m. leprae" published by dr. laszlo kato. | 1987 | 3326905 | |
| [effect of the suppression of the popliteal ganglion in the reproduction of mycobacterium leprae in the foot-pad of mice. preliminary report]. | 1987 | 3327105 | |
| [experimental leprosy]. | 1987 | 3327348 | |
| thesis and proposition for short-term and long-term support of leprosy sufferers. the leprosy bacillus must be cultivated. | 1987 | 3327349 | |
| the difficult decision when to discontinue multidrug therapy in leprosy. | 1987 | 3327350 | |
| leprosy in singapore. | the incidence of leprosy in singapore is declining over the past 20 years. more than one third of new cases are now over 50 years while no child under 5 years has been affected since 1970. male to female ratio is 2:1. indians are more significantly affected compared to the other races and a higher proportion has tuberculoid leprosy. tuberculoid leprosy accounts for 50.4% of cases seen during the past decade. case detections from household contacts is still worthwhile as it accounts for 3% to 13. ... | 1987 | 3328554 |
| enzymes for purine synthesis and scavenging in pathogenic mycobacteria and their distribution in mycobacterium leprae. | three enzymes catalysing the synthesis of four intermediates (phosphoribosylglycinamide, phosphoribosylaminoimidazole-succinocarboxamide, phosphoribosylaminoimidazole-carboxamide and amp) in the purine biosynthetic pathway were detected in extracts of mycobacterium microti and m. avium, even when the organisms had been grown in mice. however only one of the three enzymes, adenylosuccinate amp-lyase (catalysing the synthesis of the last two of the four intermediates listed above) was detected in ... | 1987 | 3328771 |
| the serological response to the phenolic glycolipid of mycobacterium leprae in australian and nepali leprosy patients. | antibodies to the species-specific phenolic glycolipid (pgl-1) of mycobacterium leprae and a crude m. leprae sonicate were measured by elisa in sera from newly diagnosed and treated leprosy patients from sydney and nepal. igm anti-pgl-1 antibodies were present in 88-90% of untreated patients at the lepromatous pole of the clinical spectrum and 35-55% of those at the tuberculoid pole. in treated patients with either form of the disease, igm anti-pgl-1 antibodies were within the normal range or mi ... | 1987 | 3328975 |
| a component of mycobacterium leprae as immunomodulating agent for immune deficient cells of leprosy patients. | the delipidified component of the insoluble portion which presumably is the cell wall of mycobacterium leprae (dcw) was able to induce lymphocyte proliferation in the leucocyte culture from the peripheral blood of lepromatous leprosy patients. normally these cells show no lymphocyte proliferation in response to m. leprae or their sonicated extract. the delipidified component (dcw) appears to be proteinaceous and able to induce antibodies in rabbit. the dcw has affinity to the sera from lepromato ... | 1987 | 3329228 |
| [a method for electron microscopic observation of human leproma]. | 1987 | 3329648 | |
| production of monoclonal antibody to a 65,000 dalton protein of mycobacterium leprae. | 1987 | 3330083 | |
| the bacteriological examination of slit-skin smears in leprosy control programmes using multiple drug therapy: a plea for radical changes in current operational methodology. | 1987 | 3330552 | |
| renal involvement in mycobacterium leprae infected mice. histopathological, bacteriological and immunofluorescence study. | swiss albino mice were inoculated in the footpads with mycobacterium leprae obtained from untreated lepromatous patient. the kidneys obtained from the animals sacrificed during different periods were processed for histopathology, presence of afb and immunofluorescence studies. renal lesions, afb and immune complex deposits were seen in the infected animals. such findings have not been studied in great detail in experimental leprosy earlier. | 1987 | 3330553 |
| the simultaneous inoculation of mycobacterium leprae and m. intracellulare into nude mice: development of cutaneous leproma and accelleration of foot pad swelling. | organisms of the non-pathogenic mycobacterium intracellulare serotype 19 darden enhanced the pathogenicity of m. leprae when inoculated together into the foot pads of nude mice. this supporting effect could be demonstrated by an accelleration of foot pad swelling, beginning 4 months after inoculation and by the development of cutaneous leproma on dorsal and lateral body sites within 6 months after inoculation. these leproma increased in number and size during the 9 months they were under observa ... | 1987 | 3330554 |
| role of immune complexes in alteration of lymphocyte subpopulation numbers and functions in experimental leprosy. | swiss albino mice (normal as well as thymectomised and irradiated were inoculated into the footpads with mycobacterium leprae and divided into two main phases of study. phase i comprised of animals not given preformed immune complexes (ic). uninfected controls were however included. phase ii consisted of animals given in vitro prepared ic at zero day period (odic), three month period (3mic) or six month period (6mic) to both uninfected and infected groups. splenic lymphocytes were isolated to qu ... | 1987 | 3330555 |
| preliminary results on the detection of mycolic acids from mycobacterium leprae by gel permeation chromatography and proton nuclear resonance spectroscopy. | 1987 | 3330748 | |
| [the leprosy bacillus: expectations for culturing and vaccination]. | 1987 | 3331128 | |
| use of phenolic glycolipid 1 for serodiagnosis of leprosy in a high prevalence village in papua new guinea. | this study reports on the usefulness of an igm phenolic glycolipid 1 (pgl-1) elisa for serodiagnosis of leprosy in the first year of a prospective longitudinal community survey in a high (8.8%) prevalence village in papua new guinea. the igm pgl-1 elisa had limited value as a screening method for detection of new cases. many normal persons, particularly children, had elevated igm anti-pgl-1 antibodies, presumably a consequence of early subclinical infection. | 1987 | 3332501 |
| eleven years of follow-up of the pattern of leprosy complications at alupe leprosy hospital. | 1987 | 3333748 | |
| purification of m. leprae with special reference to the effects of purified m. leprae vaccines on host macrophage cell functions. | 1987 | 3333939 | |
| [infection of mycobacterium leprae and mycobacterium lepraemurium into a cloned macrophage like cell line]. | 1987 | 3333940 | |
| [collection method of mycobacterium leprae from infected armadillo liver--a comparison with draper's method]. | 1987 | 3333941 | |
| [inactivation methods of mycobacterium leprae as a leprosy vaccine]. | 1987 | 3333942 | |
| towards new leprosy and tuberculosis vaccines. | leprosy and tuberculosis are chronic infectious diseases causing major global health problems, for which no effective control by vaccination has been achieved. recent advances in biotechnology may facilitate the design of a new vaccine generation. in this paper the need for, and the potential of, leprosy and tuberculosis vaccines are discussed. | 1987 | 3155274 |
| the first joint thelep-sasakawa memorial health foundation workshop on experimental chemotherapy of leprosy. | 1987 | 3125288 | |
| rifabutin (ansamycin lm 427): a new rifamycin-s derivative for the treatment of mycobacterial diseases. | rifabutin (ansamycin lm 427), a semisynthetic spiropiperidyl derivative of rifamycin s, shows good in vitro activity against most mycobacterial species, including mycobacterium avium complex. in animal models, the drug is more active against both mycobacterium tuberculosis and mycobacterium leprae than in rifampin, and studies indicate that rifabutin is active against some rifampin-resistant strains of both species. the drug has a long half-life (16 hr) in humans and a marked tissue tropism, wit ... | 1987 | 3037676 |
| a single cell assay for the study of gamma-interferon formation in leprosy patients. | the number of gamma-interferon producing cells in the peripheral blood of leprosy (ll and bt) patients and controls was studied by the reversed protein a plaque assay before and after exposure in vitro to mycobacterium leprae bacilli and epstein-barr virus (ebv). the level of spontaneous gamma-interferon production was significantly higher in bt patients compared to ll patients and controls. mycobacterium leprae induced a specific gamma-interferon response in lymphocytes from bt patients and fro ... | 1987 | 3040308 |
| epithelioid cell granuloma induction in the guinea pig by haptenated mycobacterium leprae. | fluorescein isothiocyanate (fitc)-conjugated mycobacterium leprae (fitc-m. leprae) was injected intradermally into the ears of guinea pigs and granuloma formation in the draining postauricular lymph nodes was studied. at 2 weeks, there was an increase in weights and infiltration of the draining lymph nodes in half of the animals injected with fitc-m. leprae. at 5 weeks, there was a significant increase in the weights and infiltration of these draining lymph nodes in the guinea pigs injected with ... | 1987 | 3109743 |
| structure of the major triglycosyl phenol-phthiocerol of mycobacterium tuberculosis (strain canetti). | phenol-phthiocerol glycolipids have been found previously in mycobacterium leprae, m. kansasii, m. bovis and m. marinum, but not in m. tuberculosis. a search for glycolipids in this latter species showed that the canetti strains of m. tuberculosis synthesize a major triglycosyl phenol-phthiocerol, accompanied by minor amounts of other glycolipids with a similar aglycone moiety. the triglycoside moiety has the following structure: 2,3,4-tri-o-methyl l-fucopyranosyl(alpha 1----3)l-rhamnopyranosyl( ... | 1987 | 3113946 |
| gamma interferon, but not mycobacterium leprae, induces major histocompatibility class ii antigens on cultured rat schwann cells. | in order to investigate the possible role of schwann cells in immune reactions, and in particular their involvement in the response to infection with mycobacterium leprae, it was determined under what conditions schwann cells express major histocompatibility complex class ii (mhc class ii) antigens, since these molecules are thought to have a key role in antigen presentation during cellular immune responses. in situ and in vitro preparations from newborn and adult rat sciatic nerves were used as ... | 1987 | 3114433 |
| expression of major histocompatibility complex class i and class ii antigens in human schwann cell cultures and effects of infection with mycobacterium leprae. | recent experiments on rats have raised the possibility that schwann cells can present antigens to t lymphocytes. we have investigated whether this mechanism might be relevant in leprosy by determining under what conditions human schwann cells express class i and class ii antigens, and whether infection with mycobacterium leprae affects this expression. the distribution of these antigens was examined on human schwann cells in dissociated cell cultures derived from human fetal peripheral nerves. w ... | 1987 | 3115648 |
| mycobacterial carbohydrate antigens for serological testing of patients with leprosy. | to determine whether quantitation of antibodies to mycobacterial carbohydrate determinants would be valuable in serodiagnosis and monitoring of leprosy, we tested serum igm antibody to mycobacterium leprae phenolic glycolipid i and igm and igg antibodies to mycobacterium tuberculosis and m. leprae lipoarabinomannan (lam) by enzyme-linked immunosorbent assay. seventy-one percent of patients with paucibacillary disease and 85.5% of patients with multibacillary disease were positive for at least on ... | 1987 | 3116103 |
| metabolic studies on mycobacteria. iv. assay of isocitrate lyase and malate synthase activity in m. leprae. | cell free extracts from m. tuberculosis h37 rv, m. smegmatis armadillo derived m. leprae and normal armadillo liver homogenates were assayed for the presence of isocitrate lyase and malate synthase activity. it was observed that significant amount of isocitrate lyase and malate synthase activity was present in m. tuberculosis h37 rv, m. smegmatis and armadillo derived m. leprae. no such activity was demonstrable in cell free extracts of normal armadillo liver. it is concluded that m. leprae like ... | 1987 | 3116105 |
| demonstration that the galactosyl and arabinosyl residues in the cell-wall arabinogalactan of mycobacterium leprae and mycobacterium tuberculosis are furanoid. | by a complex process involving methylation, partial hydrolysis with acid, reduction with sodium borodeuteride, ethylation, further hydrolysis and reduction, and subsequent capillary gas-liquid chromatography-mass spectrometry of the derived alditol acetates, it was established that the arabinogalactans of mycobacterium leprae and mycobacterium tuberculosis contain arabinofuranyosyl and galactofuranosyl residues exclusively. thus, the covalently bound, highly immunogenic arabinogalactan of mycoba ... | 1987 | 3119212 |
| immunological activity of a 14-kilodalton recombinant protein of mycobacterium tuberculosis h37rv. | a 14-kilodalton peptide antigen from mycobacterium tuberculosis was isolated from an escherichia coli lambda gt 11 recombinant dna clone and was identified by western blotting (immunoblotting) with monoclonal antibody tb68. immunization of mice and guinea pigs with the recombinant peptide (rtb68) induced in vitro lymphoproliferative responses in draining lymph node lymphocyte cultures as well as in vivo delayed-type hypersensitivity reactions. moreover, rtb68 was found both to induce and to cros ... | 1987 | 3119499 |
| etiological factors in delayed-type hypersensitivity reactions in leprosy. | 1987 | 3123582 | |
| beta-lactamase synthesis in mycobacterium leprae. | beta-lactam antibiotics are not active against mycobacterium leprae. the enzyme beta-lactamase mediates the most common form of bacterial resistance to penicillins and cephalosporins. cell-free extracts of purified suspensions of m. leprae were examined for beta-lactamase. the bacteria were prepared from the tissues of experimentally-infected nine-banded armadillos. most of the suspensions were inactive. however, the bacteria obtained from the tissues of armadillos treated with penicillin g benz ... | 1987 | 3295489 |
| mycobacterial antigen detection by immunohistochemistry in pulmonary tuberculosis. | a preliminary diagnosis of tuberculosis can be established by the detection of acid-fast bacilli (afb) and confirmed by culture of the microorganism. to evaluate an alternative method of diagnosis, the distribution of mycobacterial antigens in lung tissue specimens was characterized by an indirect peroxidase-antiperoxidase method and was compared to the detection of afb by ziehl-neelsen stain. histologic specimens were obtained from 59 hospital patients. of nine patients with mycobacterial disea ... | 1987 | 3297995 |
| activity of minocycline in mycobacterium leprae-infected mice. | 1987 | 3298454 | |
| assessment of the immune deficit in leprosy patients and the effect of recombinant il-2 in vitro. | although the mechanism of immunologic unresponsiveness in lepromatous leprosy remains unknown, it has been shown that interleukin-2 (il-2) production is defective in these patients. peripheral blood mononuclear cells (pbmc) were isolated from treated (less than 16 months) and untreated leprosy patients as well as household contacts; age, sex, ethnically matched control subjects; and laboratory staff. pbmc were cultured for 6 days with sonicated mycobacterium leprae (1-10 micrograms/ml), dharmend ... | 1987 | 3298472 |
| defective monocyte chemotaxis in active lepromatous leprosy. | this study of monocyte chemotaxis in leprosy patients showed a significant inverse correlation (p less than 0.05) of chemotaxis and the bacterial index (bi) (n = 22). in addition, there was a significant inverse correlation (p less than 0.05) between chemotaxis and the serum levels of anti-phenolic glycolipid-i igm antibodies (n = 20). patients taking thalidomide who had a bi greater than or equal to 1 had a significantly greater (p less than 0.001) chemotaxis response than that of patients with ... | 1987 | 3298473 |
| sensitivity and specificity of the fla-abs test for leprosy in mexican populations. | the epidemiological surveillance for leprosy must include several clinical and laboratory procedures. the fla-abs test of abe could be a useful tool for this purpose because it allows the demonstration of an effective contact with mycobacterium leprae. in order to establish the specificity, sensitivity, and predictability of the fla-abs test under mexican conditions, we studied sera collected from six groups of individuals: 60 healthy donors from a nonendemic area, 57 cases hospitalized for cond ... | 1987 | 3298474 |
| immunization of mice with mycobacterium leprae/mycobacterium bovis bcg admixtures: modulation of the acquired response to bcg. | the generation of acquired immunity to bcg in mice was compared to that in animals receiving an admixture of bcg and killed mycobacterium leprae. no significant qualitative differences were observed between the two groups in terms of their generation of delayed sensitivity, of protective t cells, and of immunological memory. in addition, the admixture was as effective as bcg in conferring protective immunity against certain nontuberculous mycobacteria, and in one case, that of m. avium 706, sign ... | 1987 | 3298475 |
| ability of the phenolic glycolipid-i antigen of m. leprae to elicit a positive mitsuda response in the armadillo (dasypus novemcinctus). | three lepromin-positive armadillos and three lepromin-negative armadillos were tested intradermally with 100 micrograms of phenolic glycolipid-i (pgl-i) in 0.1 ml of normal saline. positive delayed-hypersensitivity granulomas at 21 days in the lepromin-positive animals and negative responses in the lepromin-negative animals were obtained. these observations suggest that purified pgl-i is capable of eliciting cell-mediated immune or delayed-hypersensitivity responses in animals sensitized to myco ... | 1987 | 3298476 |
| activity of selected beta-lactam antibiotics against mycobacterium leprae. | twelve beta-lactam antibiotics were tested for activity against mycobacterium leprae growing in the foot pads of mice. two cephalosporins (7-aminocephalosporanic acid and cefuroxime) and one cephamycin (cefoxitin) showed significant activity against m. leprae, and one penicillin (mezlocillin) exerted possible growth-promoting activity. these results suggest that particular molecular structures may be required for activity against m. leprae. | 1987 | 3298478 |
| effect of interleukin-1 (il-1) and il-2 on lymphocytes from patients with leprosy. | 1987 | 3298481 | |
| an unusual case of untreated lepromatous leprosy with rare bacilli: an immunologic follow-up. | 1987 | 3298482 | |
| effect of preformed immune complexes on the course of mycobacterium leprae infection in normal mice. | 1987 | 3298483 | |
| mycobacterium leprae, probably a microbe-dependent microorganism. | 1987 | 3298484 | |
| affinity of mycobacterium leprae with lewis rat schwannoma cell line (lewis tc 98). | 1987 | 3298903 | |
| characteristics of patients in the thelep trials of chemotherapy of leprosy at bamako and chingleput. subcommittee on clinical trials of the chemotherapy of leprosy (thelep) scientific working group of the undp/world bank/who special programme for research and training in tropical diseases. | 1987 | 3298905 | |
| surveillance for naturally acquired leprosy in a nine-banded armadillo population. | samples from 77 nine-banded armadillos (dasypus novemcinctus) inhabiting a 16.7 km portion of the east atchafalaya river levee, point coupee parish, louisiana, were serologically tested and/or histopathologically examined for evidence of naturally acquired leprosy. five of 67 (7.5%) armadillos tested sero-positive with elisa test for igm class antibodies to the phenolic-glycolipid-1 antigen of mycobacterium leprae. one of 74 (1.3%) was histopathologically positive as determined by presence of ac ... | 1987 | 3295310 |
| lepromatous placentitis and intrauterine fetal infection in lepromatous nine-banded armadillos (dasypus novemcinctus). | three pregnant lepromatous armadillos along with the three sets of four fetuses and their placentae were studied histopathologically. mycobacterium leprae were present in the decidual tissue, trophoblastic cells which line the chorionic villi, and in the cells that form the internal structure of the villi. acid-fast organisms were also seen in the spleens of three fetuses. congenital infection is clearly possible in leprosy in the armadillo and may also occur in humans. | 1987 | 3795871 |
| macrophage chemotaxis in mycobacterium leprae infected mice. | 1987 | 3596680 | |
| an investigation of family size and birth order as risk factors in leprosy. | to investigate if early exposure to mycobacterium leprae is a factor determining development of hansen's disease in general, or of a particular form of the disease, we have studied the sibship size and birth order distribution of 187 leprosy cases and 528 hospitalized control patients. by the greenwood-yule analysis, a small deficit of cases in the earlier birth order in the tuberculoid form was observed. such a deficit was not observed in lepromatous leprosy patients. in the multivariate analys ... | 1987 | 3655461 |