Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| a novel cyanobacteriolytic bacterium, bacillus cereus, isolated from a eutrophic lake. | isolation and screening of cyanobacteriolytic bacteria were carried out. fifteen strains of cyano-bacteriolytic bacteria were isolated by the double layer method using the cyanobacterium, microcystis, as a sole nutrient. the isolate, n-14, showing the highest cyanobacteriolytic activity was identified as bacillus cereus based on the 16s rrna sequence. components among the extracellular products in the culture supernatant of b. cereus were responsible for the cyanobacteriolytic activity. lytic as ... | 2003 | 16233388 |
| the antimicrobial activity of extracts of the lichen cetraria aculeata and its protolichesterinic acid constituent. | in this study, the antimicrobial activity of the acetone, diethyl ether and ethanol extracts of the lichen cetraria aculeata has been investigated. the extracts were tested against twelve bacteria and eight fungi and found active against escherichia coli, staphylococcus aureus, aeromonas hydrophila, proteus vulgaris, streptococcus faecalis, bacillus cereus, bacillus subtilis, pseudomonas aeruginosa, listeria monocytogenes. no antimicrobial activity against the fungi was detected. it was determin ... | 2003 | 14713164 |
| [conjugation in bacilli]. | the review considers experimental data on the conjugal transfer of plasmids in the bacillus cereus and bacillus subtilis groups (the transfer of large self-transmissible plasmids and the mobilization of small plasmids). conjugation in bacilli is compared with conjugation in e. coli dependent on the f factor. conjugation of bacilli in their natural habitats is also discussed. | 2003 | 14679894 |
| liver abscess due to bacillus cereus: a case report. | bacillus cereus is a food-borne pathogen that causes a self-limiting gastroenteritis. we describe the case of a 72-year-old woman admitted to our hospital because of acute abdominal colic pain. over a 2-day period, her clinical condition deteriorated rapidly, with the appearance of acute abdomen. computed tomography investigation of the abdomen showed a liver abscess (diameter approximately 3 cm). at laparotomy, the abscess was found to be ruptured to the free peritoneal cavity. the final clinic ... | 2003 | 14686990 |
| [microbiological quality of muesli samples purchased in retail network]. | the estimation of microbiological quality of muesli samples was the aim of this investigation. the study included 40 samples which represented various consignments of muesli, produced in 2002 year. total number of aerobic mesophilic bacteria, yeasts and moulds, and also occurrence of pathogenic bacteria, coliforms and enterococci were determined. the systematic units of muesli fungal flora have been identified. it was stated that microbiological quality of all tested muesli was good. no pathogen ... | 2003 | 14755854 |
| the role of bacterial and non-bacterial toxins in the induction of changes in membrane transport: implications for diarrhea. | bacterial toxins induce changes in membrane transport which underlie the loss of electrolyte homeostasis associated with diarrhea. bacterial- and their secreted toxin-types which have been linked with diarrhea include: (a) vibrio cholerae (cholera toxin, e1 tor hemolysin and accessory cholera enterotoxin); (b) escherichia coli (heat stable enterotoxin, heat-labile enterotoxin and colicins); (c) shigella dysenteriae (shiga-toxin); (d) clostridium perfringens (c. perfringens enterotoxin, alpha-tox ... | 2003 | 14757199 |
| inactivation of bacillus cereus spores in milk by mild pressure and heat treatments. | 2003 | 14702650 | |
| maldi analysis of bacilli in spore mixtures by applying a quadrupole ion trap time-of-flight tandem mass spectrometer. | a novel ion trap time-of-flight hybrid mass spectrometer (qit-tof ms) has been applied for peptide sequencing in proteolytic digests generated from spore mixtures of bacilli. the method of on-probe solubilization and in situ proteolytic digestion of small, acid-soluble spore proteins has been recently developed in our laboratory, and microorganism identification in less than 20 min was accomplished. in this study, tryptic peptides were generated in situ from complex spore mixtures of b. subtilis ... | 2003 | 14710845 |
| characterization of bacillus spore species and their mixtures using postsource decay with a curved-field reflectron. | a strategy is proposed for the rapid identification of bacillus spores, which relies on the selective release of a family of proteins, referred to as small, acid-soluble spore proteins (sasps). in this work, sasps were selectively solubilized from bacillus spores on the maldi sample plate by using 10% tfa. proteolytic digests of sasps generated in situ from spores of b. subtilis 168, b. globigii, b. thuringiensis subs. kurstaki hd-1, b. cereus t, and the nonpathogenic strain b. anthracis sterne ... | 2003 | 14710846 |
| asymmetric reduction of benzil to (s)-benzoin with whole cells of bacillus cereus. | benzil (1) was selectively reduced to (s)-benzoin (2) in the presence of a wild-type bacillus cereus tim-r01. a 92% yield of 2 with 94% enantiomeric excess ratio was attained in phosphate-buffered saline (pbs) (ph 7.5) by using glucose as a nutrient at 37 degrees c for 12 h. compound 2 was not reduced further to hydrobenzoin (3) at all. the reduction activity differed greatly depending on the strain of b. cereus. under these conditions the b. cereus strains ifo3001, ifo15305, iam1110, iam1229, i ... | 2003 | 14665737 |
| cytotoxicity and antimicrobial activity of goniothalamin isolated from bryonopsis laciniosa. | the cytotoxicity and antimicrobial activity of goniothalamin isolated from bryonopsis laciniosa was investigated. goniothalamin showed potent cytotoxicity with lc50 values (5.03 microg/ml) comparable with the reference standard agent, gallic acid. it also showed weak antibacterial and significant antifungal activity against a wide range of gram positive and gram negative bacteria and fungi. the antibacterial (minimum inhibitory concentration) effect against bacillus cereus and shigella shiga was ... | 2003 | 14669248 |
| treatment of saline wastewaters from marine-products processing factories by activated sludge reactor. | an activated sludge reactor, operated at room temperature (20-30 degrees c) was used to treat saline wastewaters generated by marine-products industries. the system was operated continuously and the influence of the organic loading rates (olrs), varying from 250 to 1000 mg cod l(-1) day(-1), on chemical oxygen demand (cod) removal was investigated. the system, inoculated with nacl-acclimated culture, removed up to 98% and 88% of the influent cod concentrations at olrs of 250 and 1000 mg cod l(-1 ... | 2003 | 14669806 |
| expression of binary toxin genes in the mosquito-colonizable bacteria, bacillus cereus, leads to high toxicity against culex quinquefasciatus larvae. | two b. cereus strains, ae10 and cx5, isolated from mosquito larval guts, were transformed with a recombinant plasmid, pbs373, harboring binary toxin genes from bacillus sphaericus 2297. immunoblotting analysis clearly revealed the production and presence of the 51-kda toxin protein in both strains. two recombinant b. cereus strains ae10 and cx5 showed very high toxicity against c. quinquefasciatus larvae. since both strains have a close relationship with the mosquito larvae in the native environ ... | 2003 | 14669911 |
| synthesis of 6-fluoro-1,4-dihydro-4-oxo-quinoline-3-carboxylic acid derivatives as potential antimicrobial agents. | in the present study, a series of 1-ethyl/benzyl-6-fluoro-7-(substituted piperazin-1-yl)1,4-dihydro-4-oxo-quinoline-3-carboxylic acid were synthesized and characterized by ir, 1h-nmr, mass spectral and elemental analysis. the in vitro antibacterial and antifungal activities of the compounds were evaluated by paper disc diffusion method. the minimum inhibitory concentrations (mic) of the compounds were also determined by agar streak dilution method. the in vivo antibacterial activity of the compo ... | 2003 | 14642332 |
| antibacterial activity and mechanism of action of tick defensin against gram-positive bacteria. | defensins are a major group of antimicrobial peptides and are found widely in vertebrates, invertebrates and plants. invertebrate defensins have been identified from insects, scorpions, mussels and ticks. in this study, chemically synthesized tick defensin was used to further investigate the activity spectrum and mode of action of natural tick defensin. synthetic tick defensin showed antibacterial activity against many gram-positive bacteria but not gram-negative bacteria and low hemolytic activ ... | 2003 | 14642822 |
| conformational changes induced by cloxacillin in class a beta-lactamase from bacillus cereus. | class a beta-lactamases are enzymes that hydrolyse beta-lactam antibiotics such as penicillins and cephalosporins. they also hydrolyse substrate analogues such as oxacillin and cloxacillin, with a biphasic kinetic as it has been reported for bacillus cereus beta-lactamase. a molecular model of bacillus cereus beta-lactamase was built and the conformational changes that the substrates benzylpenicillin and cloxacilline produced in the conformation of selected regions of the protein were analyzed. ... | 2003 | 14656057 |
| fatal bacillus cereus meningitis without inflammatory reaction in cerebral spinal fluid after bone marrow transplantation. | 2003 | 14657704 | |
| inactivation of geobacillus stearothermophilus spores by high-pressure carbon dioxide treatment. | high-pressure co2 treatment has been studied as a promising method for inactivating bacterial spores. in the present study, we compared this method with other sterilization techniques, including heat and pressure treatment. spores of bacillus coagulans, bacillus subtilis, bacillus cereus, bacillus licheniformis, and geobacillus stearothermophilus were subjected to co2 treatment at 30 mpa and 35 degrees c, to high-hydrostatic-pressure treatment at 200 mpa and 65 degrees c, or to heat treatment at ... | 2003 | 14660357 |
| real-time molecular beacon nasba reveals hblc expression from bacillus spp. in milk. | nucleic acid sequence-based amplification (nasba) was applied in combination with a fluorescein-conjugated molecular beacon specific for a sequence flanked by transcript-specific primers in order to monitor hblc enterotoxin gene expression in real-time from milk separately contaminated with bacillus amyloliquefaciens, bacillus cereus, and bacillus circulans. maximal enterotoxin expression was noted following 16, 15, and 16 h, respectively, when grown in artificially contaminated nonfat dried mil ... | 2003 | 14592426 |
| aerobic tryptophan degradation pathway in bacteria: novel kynurenine formamidase. | while a variety of chemical transformations related to the aerobic degradation of l-tryptophan (kynurenine pathway), and most of the genes and corresponding enzymes involved therein have been predominantly characterized in eukaryotes, relatively little was known about this pathway in bacteria. using genome comparative analysis techniques we have predicted the existence of the three-step pathway of aerobic l-tryptophan degradation to anthranilate (anthranilate pathway) in several bacteria. based ... | 2003 | 14592712 |
| inactivation of spores of bacillus cereus in cheese by high hydrostatic pressure with the addition of nisin or lysozyme. | the objective of this work was to study high hydrostatic pressure (hhp) inactivation of spores of bacillus cereus atcc 9139 inoculated in model cheeses made of raw milk, together with the effects of the addition of nisin or lysozyme. the concentration of spores in model cheeses was approximately 6-log10 cfu/g of cheese. cheeses were vacuum packed and stored at 8 degrees c. all samples except controls were submitted to a germination cycle of 60 mpa at 30 degrees c for 210 min, to a vegetative cel ... | 2003 | 14594224 |
| wessex shopping basket survey - a structured approach to local food sampling. | the shopping basket approach to food sampling, in which ready-to-eat food items from a shopping list were sampled at premises chosen at random, was adopted by 15 environmental health departments in the wessex region. a total of 2037 samples were analysed over a 30-month period. the microbiological quality varied considerably between food categories, with gravy and stock samples giving the highest proportion of satisfactory results. sliced meats, cooked rice and sandwiches gave the poorest overal ... | 2003 | 14594701 |
| composition and antimicrobial activity of achillea clavennae l. essential oil. | the volatile constituents of achillea clavennae l. (asteraceae), rare plant of europe, have been analysed using gc/ms. twenty- five components making up 81.6% of the oil were characterized with camphor (29.5%), myrcene (5.5%), 1,8-cineole (5.3%), beta-caryophyllene (5.1%) and linalool (4.9%) being the major constituents. the essential oil was evaluated for antibacterial and antifungal activities. the screening of the antimicrobial activity of essential oil was conducted by a disc diffusion test ... | 2003 | 14595583 |
| microbial diversity and prevalence of virulent pathogens in biofilms developed in a water reclamation system. | bacterial biofilm is a common phenomenon in both natural and engineered systems which often becomes a source of contamination and microbially influenced corrosion. it is thought that formation of biofilm in the monoculture of several bacterial species is regulated by acylhomoserine lactone (ahl) quorum-sensing signals. in this study, we investigated the microbial diversity and existence of ahl-producing and ahl-degrading bacterial species in the biofilm samples from a water reclamation system lo ... | 2003 | 14596899 |
| microbial and copper adsorption by smectitic clay--an experimental study. | the objective of this study was to quantify copper-, bacteria- and bacteriophage-binding capacities of natural clay with the aim of predicting the adsorption of heavy metals, human pathogenic bacteria and viruses by a clayey landfill liner. x-ray diffraction analysis of six natural clays showed that the dominant phase in all deposits consists of smectites together with illite, kaolin and, sometimes, palygorskite and sepiolite. the specific surface areas of different clay substrates were very hig ... | 2003 | 14599145 |
| in vitro assay for human toxicity of cereulide, the emetic mitochondrial toxin produced by food poisoning bacillus cereus. | the in vitro boar spermatozoon test was compared with the lc ion trap ms analysis for measuring the cereulide content of a pasta dish, implemented in serious emetic food poisoning caused by bacillus cereus. both assays showed that the poisonous food contained approximately 1.6 microg of cereulide g(-1) implying the toxic dose in human as < or =8 microg kg(-1) body weight. the threshold concentration of cereulide provoking visible mitochondrial damage in boar sperm exposed in vitro was 2 ng of ce ... | 2003 | 14599471 |
| identification of genomic islands in the genome of bacillus cereus by comparative analysis with bacillus anthracis. | horizontal gene transfer has been recognized as a universal event throughout bacterial evolution. the availability of both complete genome sequences of bacillus cereus and b. anthracis provides the possibility to perform comparative analysis based on their genomes. by using a windowless method to display the distribution of the genomic gc content of b. cereus and b. anthracis, we have found three genomic islands in the genome of b. cereus, i.e., bcgi-1, bcgi-2, and bcgi-3, respectively, which ar ... | 2003 | 14600214 |
| duplex real-time sybr green pcr assays for detection of 17 species of food- or waterborne pathogens in stools. | a duplex real-time sybr green lightcycler pcr (lc-pcr) assay with dna extraction using the qiaamp dna stool mini kit was evaluated with regard to detection of 8 of 17 species of food- or waterborne pathogens in five stool specimens in 2 h or less. the protocol used the same lc-pcr with 20 pairs of specific primers. the products formed were identified based on a melting point temperature (t(m)) curve analysis. the 17 species of food- or waterborne pathogens examined were enteroinvasive escherichi ... | 2003 | 14605150 |
| identification of foodborne bacteria by infrared spectroscopy using cellular fatty acid methyl esters. | identification of bacterial species by profiling fatty acid methyl esters (fames) has commonly been carried out by using a 20-min capillary gas chromatographic procedure followed by library matching of fame profiles using commercial midi databases and proprietary pattern recognition software. fast gc (5 min) fame procedures and mass spectrometric methodologies that require no lipid separation have also been reported. in this study, bacterial identification based on the rapid (2 min) infrared mea ... | 2003 | 14607413 |
| interaction of phospholipases c and sphingomyelinase with liposomes. | 2003 | 14610804 | |
| genes for an alkaline d-stereospecific endopeptidase and its homolog are located in tandem on bacillus cereus genome. | alkaline d-peptidase (adp) from bacillus cereus df4-b is a d-stereospecific endopeptidase acting on oligopeptides composed of d-phenylalanine and the primary structure deduced from its gene, adp, shows a similarity with d-stereospecific hydrolases from ochrobactrum anthropi strains. we have isolated dna fragments covering the flanking region of adp from df4-b genome and found an additional gene, adp2, located upstream of adp. the deduced amino acid sequence of adp2 showed 96% and 85% identity wi ... | 2003 | 14612229 |
| nosocomial bacteremia and catheter infection by bacillus cereus in an immunocompetent patient. | we present a case of bacillus cereus bacteremia and catheter infection in an immunocompetent patient subjected to abdominal surgery, who recovered following central catheter removal and treatment with piperacillin/tazobactam. | 2003 | 14616690 |
| the bacillus thuringiensis linear double-stranded dna phage bam35, which is highly similar to the bacillus cereus linear plasmid pbclin15, has a prophage state. | bam35, a 15-kbp double-stranded dna phage, infects bacillus thuringiensis. recently, sequencing of the related bacillus cereus revealed a 15.1-kbp linear plasmid, pbclin15. we show that pbclin15 closely resembles bam35 and demonstrate conversion of bam35 to a prophage. this state is common, as several b. thuringiensis strains release bam35-related viruses. | 2003 | 14617663 |
| inhibition of bacillus cereus by strains of lactobacillus and lactococcus in milk. | the growth and death or survival of bacillus cereus in sterile skimmed milk fermented with 18 different lactic acid bacteria (lab) were investigated. b. cereus alone in milk reached about 10(7)-10(8) colony-forming units (cfu)/ml. when b. cereus was cultivated together with different lactobacillus or lactococcus cultures at 30 or 37 degrees c, the b. cereus counts after 72 h of fermentation ranged between < 10 cfu/ml and about 10(6) cfu/ml. the inhibition patterns for the different lactobacillus ... | 2003 | 14623386 |
| effect of gamma irradiation on the survival of pathogens in kwamegi, a traditional korean semidried seafood. | kwamegi (semidried raw pacific saury) is traditional seafood available in korea. it has water activity in the range of 0.90 to 0.95. spoilage and the growth of most pathogenic bacteria is retarded because of low water activity, low temperature, and packaging. however, it is contaminated with bacteria of public health significance and poses a hazard to the consumer because it is consumed raw without any cooking. the effectiveness of these hurdles in preventing the growth of staphylococcus aureus, ... | 2003 | 14627288 |
| a bacillus thuringiensis isolate possessing a spore-associated filament. | we report on a novel bacterium, isolated during a screen for environmental isolates of bacillus thuringiensis, that possesses a novel filamentous structure. nucleotide sequence from the isolate's 16s rrna gene places the bacterium unambiguously within the bacillus thuringiensis/bacillus cereus group. phase-contrast and electron microscopy indicate the presence of both a parasporal body and a long filament which are retained after sporulation. the filament is shown to consistently arise from the ... | 2003 | 14629020 |
| rapid determination of vitamin b2 secretion by bacteria growing on solid media. | development of an agar-diffusion assay to measure vitamin b2 in biological samples and application of the method to determine the amount of vitamin b2 secreted by bacteria. | 2003 | 14632999 |
| a molecular method to detect bacillus cereus from a coffee concentrate sample used in industrial preparations. | the aim of this work was to develop specific primers which are able to detect bacillus cereus in a coffee concentrate sample. | 2003 | 14633011 |
| detection and characterization of the novel bacteriocin entomocin 9, and safety evaluation of its producer, bacillus thuringiensis ssp. entomocidus hd9. | to identify and characterize new bacteriocins from a collection of 41 strains belonging to 27 subspecies of bacillus thuringiensis, and to evaluate the safety of the producers. | 2003 | 14633027 |
| genehunter, a transposon tool for identification and isolation of cryptic antibiotic resistance genes. | genehunter is a transposon tool designed for the experimental activation and identification of silent antibiotic resistance genes. the method permits the identification of novel resistance genes that lack previously identified homologues. using salmonella enterica serovar typhimurium strain lt2 as a test organism for the in vivo version of the genehunter method, we were able to activate, clone, and identify two cryptic antibiotic resistance genes, the aminoglycoside acetyltransferase aac(6')-iaa ... | 2003 | 14638492 |
| lethal gastroenteritis associated with clozapine and loperamide. | 2003 | 14638602 | |
| application of sonication to release dna from bacillus cereus for quantitative detection by real-time pcr. | a rapid sonication method for lysis of gram-positive bacteria was evaluated for use in combination with quantitative real-time polymerase chain reaction (pcr) analyses for detection. other criteria used for evaluation of lysis were microscopic cell count, colony forming units (cfu), optical density at 600 nm and total yield of dna measured by picogreen fluorescence. the aim of this study was complete disruption of cellular structures and release of dna without the need for lysing reagents and ti ... | 2003 | 14499990 |
| development of a rapid 1-h fluorescence-based cytotoxicity assay for listeria species. | listeria monocytogenes is cytotoxic to the lymphocyte-origin hybridoma ped-2e9 cell line. the relative cytotoxicity can be calculated by assaying the release of alkaline phosphatase (alp) from the infected cell line. in this study, a fluorogenic substrate (4-methylumbelliferyl phosphate, mup) was used to quantify the alp activity. the assay is 3.5-fold more sensitive than the colorimetric-based assay and requires only 1 h to differentiate virulent from avirulent strains. in addition to various l ... | 2003 | 14499993 |
| interaction of a free-living soil nematode, caenorhabditis elegans, with surrogates of foodborne pathogenic bacteria. | free-living nematodes may harbor, protect, and disperse bacteria, including those ingested and passed in viable form in feces. these nematodes are potential vectors for human pathogens and may play a role in foodborne diseases associated with fruits and vegetables eaten raw. in this study, we evaluated the associations between a free-living soil nematode, caenorhabditis elegans, and escherichia coli, an avirulent strain of salmonella typhimurium, listeria welshimeri, and bacillus cereus. on an a ... | 2003 | 14503703 |
| pseudo-outbreak of toxigenic bacillus cereus isolated from stools of three patients with diarrhoea after oral administration of a probiotic medication. | from december 2000 to january 2001 toxigenic bacillus cereus was isolated from stools of three patients with diarrhoea at two tertiary hospitals in southwest germany. two cases with nosocomial diarrhoea were apparently epidemiologically related (same time and ward), a third case was unrelated with respect to time and location. in order to investigate the epidemiology of these three cases, clinical isolates and isolates from an unexpected, possible common source (probiotic medication) were compar ... | 2003 | 14505607 |
| complexation of cyclic dodecadepsipeptide, cereulide with ammonium salts. | cereulide is a principal toxin causing emetic syndrome which is produced by bacillus cereus and has been known as potassium selective ionophore. this paper deals with its complexation with inorganic and organic ammonium ions to assign the higher structures similar to the complex with potassium ion by means of nmr and esi-ms spectroscopy. of particular interest, the detectable ions are not only at m/z 1191.8 as k(+) complex but also (or sometimes exclusively) at m/z 1170.8 as nh(4)(+) complex in ... | 2003 | 14505659 |
| an antifungal chitinase produced by bacillus cereus with shrimp and crab shell powder as a carbon source. | the production of inexpensive chitinolytic enzymes is an element in the utilization of shellfish processing wastes. in this study, shrimp and crab shell powder prepared by treating shrimp and crab processing wastes with boiling and crushing was used as a substrate for the isolation of an antifungal chitinase-producing microorganism. bacillus cereus yq 308, a strain isolated from the soil samples, excreted one chitinase when cultured in a medium containing 2% (wt/vol) shrimp and crab shell powder ... | 2003 | 14506855 |
| an efficient and selective enzymatic oxidation system for the synthesis of enantiomerically pure d-tert-leucine. | [reaction: see text] d-tert-leucine was prepared with an enantiomeric excess of >99% by an enzyme-catalyzed oxidative resolution of the racemic mixture of dl-tert-leucine with use of leucine dehydrogenase. the l-amino acid was oxidized completely due to coupling of the primary reaction with a highly efficient irreversible nad(+)-regenerating step by nadh oxidase. | 2003 | 14507195 |
| diacylglycerol-rich domain formation in giant stearoyl-oleoyl phosphatidylcholine vesicles driven by phospholipase c activity. | we have studied the effect of phospholipase c from bacillus cereus and clostridium perfringens (alpha-toxin) on giant stearoyl-oleoyl phosphatidylcholine (sopc) vesicles. enzyme activity leads to a binary mixture of sopc and the diacylglycerol sog, which phase separates into a sopc-rich bilayer phase and a sog-rich isotropic bulk-like domain embedded within the membrane, as seen directly by phase contrast microscopy. after prolonged enzymatic attack, all bilayer membranes are transformed into an ... | 2003 | 14507699 |
| antibacterial and proteolytic activity in venom from the endoparasitic wasp pimpla hypochondriaca (hymenoptera: ichneumonidae). | venom from the endoparasitic wasp, pimpla hypochondriaca, is composed of a mixture of high and low molecular weight proteins, possesses phenoloxidase activity, has immunosuppressive properties, and induces paralysis in several insect species. in the present study we demonstrate that p. hypochondriaca venom also contains antibacterial and proteolytic activity. antibacterial activity was detected against the gram-negative bacteria escherichia coli and xanthamonas campestris but not against pseudom ... | 2003 | 14511827 |
| use of bromodeoxyuridine immunocapture to identify active bacteria associated with arbuscular mycorrhizal hyphae. | arbuscular mycorrhizae are beneficial for crops grown under low-till management systems. increasingly, it is becoming apparent that bacteria associated with mycorrhizae can enhance the beneficial relationship between mycorrhizae and plants. however, it has been difficult to study these relationships by conventional techniques. in this study actively growing bacteria were identified in soil from an undisturbed fallow field known to contain arbuscular mycorrhizae by using molecular tools to elimin ... | 2003 | 14532082 |
| use of denaturing high-performance liquid chromatography to identify bacillus anthracis by analysis of the 16s-23s rrna interspacer region and gyra gene. | denaturing high-performance liquid chromatography (dhplc) was evaluated as a method for identifying bacillus anthracis by analyzing two chromosomal targets, the 16s-23s intergenic spacer region (isr) and the gyra gene. the 16s-23s isr was analyzed by this method with 42 strains of b. anthracis, 36 strains of bacillus cereus, and 12 strains of bacillus thuringiensis; the gyra gene was analyzed by this method with 33 strains of b. anthracis, 27 strains of b. cereus, and 9 strains of b. thuringiens ... | 2003 | 14532217 |
| a new multiplex pcr for the detection of hbl genes in strains of the 'bacillus cereus group'. | 2003 | 14535496 | |
| morphogenesis of bacillus spore surfaces. | spores produced by bacilli are encased in a proteinaceous multilayered coat and, in some species (including bacillus anthracis), further surrounded by a glycoprotein-containing exosporium. to characterize bacillus spore surface morphology and to identify proteins that direct formation of coat surface features, we used atomic-force microscopy (afm) to image the surfaces of wild-type and mutant spores of bacillus subtilis, as well as the spore surfaces of bacillus cereus 569 and the sterne strain ... | 2003 | 14563859 |
| microbial ecology of bacillus thuringiensis: fecal populations recovered from wildlife in korea. | a total of 34 fecal samples, collected from 14 species of wild mammals in korea, were examined for the occurrence of bacillus thuringiensis. the organism was detected in 18 (53%) samples. among the three food-habit groups, herbivorous animals yielded the highest frequency (69%) of samples positive for b. thuringiensis, followed by omnivorous animals (50%). of the six fecal samples from carnivorous animals, only one sample contained b. thurin giensis. among 527 isolates belonging to the bacillus ... | 2003 | 14569287 |
| bactericidal effects of konjac fluid on several food-poisoning bacteria. | in this study, the bactericidal effects of japanese alkaline foods on food-poisoning bacteria were evaluated. konjac is an alkaline food soaked in calcinated calcium (the ph of konjac fluid ranges from 11.42 to 12.53). konjac fluids completely inactivated escherichia coli, enterohemorrhagic e. coli o157:h7 and e. coil o26:h9, salmonella enteritidis, vibrio parahemolyticus. and staphylococcus aureus. the initial level of 6 log cfu/ml dramatically decreased after incubation with konjac fluid, and ... | 2003 | 14572219 |
| consumer food-handling behaviors associated with prevention of 13 foodborne illnesses. | to be effective in reducing the incidence of foodborne illness, consumers and food safety educators need information about behaviors that will decrease exposure to foodborne pathogens. a four-round delphi technique was used to survey nationally recognized experts in food microbiology, epidemiology, food safety education, and food safety policy with the aim of identifying and ranking food-handling and consumption behaviors associated with 13 major foodborne pathogens. the food safety experts rank ... | 2003 | 14572229 |
| phosphatidylcholine-specific phospholipase c and sphingomyelinase activities in bacteria of the bacillus cereus group. | bacillus anthracis is nonhemolytic, even though it is closely related to the highly hemolytic bacillus cereus. hemolysis by b. cereus results largely from the action of phosphatidylcholine-specific phospholipase c (pc-plc) and sphingomyelinase (sph), encoded by the plc and sph genes, respectively. in b. cereus, these genes are organized in an operon regulated by the global regulator plcr. b. anthracis contains a highly similar cereolysin operon, but it is transcriptionally silent because the b. ... | 2003 | 14573681 |
| ceramide is involved in r(+)-methanandamide-induced cyclooxygenase-2 expression in human neuroglioma cells. | cannabinoids have recently been shown to induce the expression of the cyclooxygenase-2 (cox-2) isoenzyme in h4 human neuroglioma cells. using this cell line, the present study investigates the contribution of the second messenger ceramide to this signaling pathway. incubation of cells with the endocannabinoid analog r(+)-methanandamide (r(+)-ma) was associated with an increase of intracellular ceramide levels. enhancement of ceramide formation by r(+)-ma was abolished by fumonisin b1, a ceramide ... | 2003 | 14573769 |
| microbial transformation of cortisol and prolyl endopeptidase inhibitory activity of its transformed products. | incubation of cortisol (1) with gibberella fujikuruoi for 12 days yielded an oxidatively cleaved product, 11beta-hydroxyandrost-4-en-3,17-dione (2), while incubation with bacillus subtilis and rhizopus stolonifer yielded the reduced product, 11beta, 17alpha,20,21-tetrahydroxy-(20s)-pregn-4-en-3-one (3). other reduced products, 11beta, 17alpha, 21-trihydroxy-5alpha-pregnan-3, 20-dione (4) and 3beta, 11beta, 17alpha, 21-tetrahydroxy-5alpha-pregnan-20-one (5) were obtained by incubation of compound ... | 2003 | 14577687 |
| antibacterial activity of the fourth-generation fluoroquinolones gatifloxacin and moxifloxacin against ocular pathogens. | the ideal ophthalmic anti-infective exhibits broad-spectrum activity against gram-positive, gram-negative, and atypical bacterial species. these pathogens can cause potentially blinding infections such as keratitis and endophthalmitis, both of which are associated with ophthalmic surgery or traumatic injury. these infections often require aggressive antibacterial therapy, preferably with newer generations of antibiotics. in this study, minimal inhibitory concentration (mic) values for gatifloxac ... | 2003 | 14964344 |
| [organic extracts with antimicrobian activity from penicillium sp. (moniliales) isolated from the sponge ircinia felix (porifera: demospongiae)]. | this research expresses the potential of the bacterial activity present in the organic extracts obtained from penicillium sp., isolated from the esponge irciniafelix. this activity was evaluated through agar diffusion test and minimal inhibitory concentration (mic). the susceptibility trials of organic fractions were carried out against staphylococcus aureus, s. epidermidis, bacillus cereus and b. subtilis. the use of the chromatographic techniques (clv and tlc), permitted to obtain bioactive or ... | 2003 | 15264564 |
| [evaluation of a colorimetric method for studying the interaction between microorganisms and intestinal epithelial cells]. | the aim of the present study was to gain further insight on the reliability of the colorimetric determination of the activity of bacterial nitrate reductase to evaluate bacterial concentrations and interaction between microorganisms and enterocyte-like cells. nitrite produced after incubation of the samples with a nitrate-formate solution was determined with a diazotization reaction with sulphanilic acid and n-naphthyl-ethylene-diamonium dichloride. cell association assays were performed with di ... | 2003 | 14976870 |
| antibacterial effects of carica papaya fruit on common wound organisms. | the purpose of the study was to investigate antibacterial activity of ripe and unripe carica papaya on selected micro-organisms. cultures of micro-organisms were routinely maintained in nutrient agar slants at 4 degrees c. extracts of immature, mature and ripe carica papaya fruit were obtained by separately grinding factions of the epicarp, endocarp and seeds and filtering them through gauze. sensitivity tests were conducted by adding 0.06 ml of extract to agar wells (6 mm diameter) prepared fro ... | 2003 | 15040064 |
| the inhibitory effect of garlic (allium sativum) on growth of some microorganisms. | the aim of the present study was to compare between the anti-microbial activity of fresh garlic bulbs and that of its water extract against the potent food pathogens staphylococcus aureus, esherichia coil, salmonella typhi and bacillus cereus. garlic segments with different concentrations (1%, 5%, and 10%) were used. another 100 gms of garlic segments were weighed and mixed with 100 ml distilled water to prepare water extract of garlic, then 1000, 500 and 100 microg/ml were prepared. these conce ... | 2003 | 17219900 |
| thermal properties of bacterial spores and biopolymers. | differential scanning calorimetry (dsc) measurements of dormant bacterial spores is traditionally associated with an endothermic transition at around 50 degrees c. this endothermic transition was described as an indicator for two main physico-chemical states in spores. these were a glassy state in the dormant spore core as a model for spore dormancy and a heat-activated state that generally facilitates spore resuscitation. the idea of a glassy state in dormant spores is based on the observation ... | 2003 | 12381399 |
| antibacterial activity of extracts from some edible plants commonly consumed in asia. | extracts of edible plants (26 species) from china, japan, thailand and yemen were screened for their antibacterial activity against bacillus cereus, staphylococcus aureus, listeria monocytogenes, escherichia coli and salmonella infantis. buffered methanol (80% methanol and 20% pbs) and acetone extracted inhibitory substances against tested bacteria from 16 plants, as revealed by the disc assay. the minimum inhibitory concentrations (mics) of extracts determined by the agar dilution method ranged ... | 2003 | 12423924 |
| microbiological quality of 18 degrees c ready-to-eat food products sold in taiwan. | a total of 164 samples of 18 degrees c ready-to-eat (rte) food products, purchased in 1999-2000 from convenience stores and supermarkets in central taiwan, were examined to determine the microbiological quality of these products. the 18 degrees c rte food products, manufactured by 16 factories, were divided into groups based on the type of food and their major ingredients. aerobic plate count, coliforms, escherichia coli, bacillus cereus, staphylococcus aureus and psychrotrophic pseudomonas spp. ... | 2003 | 12423926 |
| isolation of nisin-producing lactococcus lactis wnc 20 strain from nham, a traditional thai fermented sausage. | a total of 14,020 lactic acid bacteria (lab) were isolated from nham and screened for bacteriocin production. one lactococcus lactis strain wnc 20 produced a bacteriocin that not only inhibited closely related lab, but also some food-borne pathogens including listeria monocytogenes, clostridium perfringens, bacillus cereus and staphylococcus aureus. biochemical studies revealed that the bacteriocin was heat-stable even at autoclaving temperature (121 degrees c for 15 min) and was active over a w ... | 2003 | 12457588 |
| bacillus cereus, bacillus thuringiensis and bacillus mycoides differentiation using a pcr-re technique. | a method was developed to differentiate between bacillus cereus, bacillus mycoides and bacillus thuringiensis using the polymerase chain reaction combined with a restriction endonuclease (pcr-re) technique. this fast and simple protocol, applied to pure culture strains, was developed using the gyrb dna sequence, as previously proposed by other authors. strains from international collections were used to optimize the method which was then applied to the identification of strains isolated from foo ... | 2003 | 12485751 |
| identification of aerobic mesophilic bacilli isolated from board and paper products containing recycled fibres. | to identify aerobic mesophilic bacteria isolated from coreboard, kitchen roll paper and food packaging boards containing recycled fibres and to create a rapid fingerprint-based database for their identification. | 2003 | 12492920 |
| crystal structure and site-directed mutagenesis of enzymatic components from clostridium perfringens iota-toxin. | iota-toxin from clostridium perfringens type e is an adp-ribosylating toxin (adprt) that adp-ribosylates actin, which is lethal and dermonecrotic in mammals. it is a binary toxin composed of an enzymatic component (ia) and a binding component (ib). ia adp-ribosylates g-actin at arginine 177, resulting in the depolymerization of the actin cytoskeleton. here, we report on studies of the structure-function relationship by the crystal structures of ia complexed with nadh and nadph (at 1.8 a and 2.1 ... | 2003 | 12498797 |
| enterotoxigenicity and cytotoxicity of bacillus thuringiensis strains and development of a process for cry1ac production. | bacillus thuringiensis is indistinguishable from bacillus cereus except for the production of insecticidal crystal proteins (icps). b. thuringiensis strains may show enterotoxin profiles and toxin levels similar to those of b. cereus strains isolated from food-poisoning cases. it is important for the food industry and farmers to consider that with the application of b. thuringiensis strains to crops, their spores may be introduced into the human food chain. in this study, 59 b. thuringiensis str ... | 2003 | 12502392 |
| eggshell matrix proteins as defense mechanism of avian eggs. | this study focused on the role of eggshell matrix proteins as a function of potential natural antimicrobial defenses of avian eggs. the electrophoretic profile of sds-page showed that the soluble eggshell matrix proteins had three major bands of 15 000, 36 000, and 66 000 and several minor bands comprising 17 000, 25 000, 30 000, and 75 000, while insoluble matrix proteins were consisting of various bands comprising at least 16 distinct migration bands between 10 000 and 200 000. three bacteria ... | 2003 | 12502416 |
| influence of ph and temperature on growth of bacillus cereus in vegetable substrates. | bacillus cereus is a food-borne pathogen which most often contaminates foods of plant origin. spores of psychrotrophic strains have the ability to germinate and grow at refrigeration temperatures in different vegetable substrates, such as carrot broth, zucchini broth, and cooked carrot purée. in some circumstances, factors such as ph, heat treatment, and storage temperature play a fundamental role in controlling the growth of these psychrotrophic strains and in extending the shelf life of refrig ... | 2003 | 12505461 |
| bacillus anthracis diverges from related clades of the bacillus cereus group in 16s-23s ribosomal dna intergenic transcribed spacers containing trna genes. | mung bean nuclease treatment of 16s-23s ribosomal dna intergenic transcribed spacers (its) amplified from several strains of the six species of the bacillus cereus group showed that b. anthracis davis te702 and b. mycoides g2 have other intermediate fragments in addition to the 220- and 550-bp homoduplex fragments typical of the b. cereus group. long and intermediate homoduplex its fragments from strains davis te702 and g2 and from another 19 strains of the six species were sequenced. two main t ... | 2003 | 12513974 |
| production of proinflammatory mediators by indoor air bacteria and fungal spores in mouse and human cell lines. | we compared the inflammatory and cytotoxic responses caused by household mold and bacteria in human and mouse cell lines. we studied the fungi aspergillus versicolor, penicillium spinulosum, and stachybotrys chartarum and the bacteria bacillus cereus, pseudomonas fluorescens, and streptomyces californicus for their cytotoxicity and ability to stimulate the production of inflammatory mediators in mouse raw264.7 and human 28sc macrophage cell lines and in the human a549 lung epithelial cell line i ... | 2003 | 12515684 |
| application of a real-time biosensor to detect bacteria in platelet concentrates. | a spore-based biosensor for detecting low levels of bacteria in real-time has been recently developed. the system (termed lexsas, label-free exponential signal-amplification system) exploits spore's ability to produce fluorescence when sensing neighboring bacterial cells. we studied the lexsas as a possible approach for identifying bacterially contaminated platelet concentrates prior to transfusion because the system offers rapid analysis, high sensitivity, and low cost. if successful, this appr ... | 2003 | 12480543 |
| dna detection and signal amplification via an engineered allosteric enzyme. | rapid, sensitive, and sequence-specific dna detection can be achieved in one step using an engineered intrasterically regulated enzyme. the semi-synthetic inhibitor-dna-enzyme (ide) construct (left) rests in the inactive state but upon exposure to a complementary dna sequence undergoes a dna hybridization-triggered allosteric enzyme activation (right). the ensuing rapid substrate turnover provides the built-in signal amplification mechanism for detecting approximately 10 fmol dna in less than 3 ... | 2003 | 12517141 |
| beta-lactamase genes of the penicillin-susceptible bacillus anthracis sterne strain. | susceptibility to penicillin and other beta-lactam-containing compounds is a common trait of bacillus anthracis. beta-lactam agents, particularly penicillin, have been used worldwide to treat anthrax in humans. nonetheless, surveys of clinical and soil-derived strains reveal penicillin g resistance in 2 to 16% of isolates tested. bacterial resistance to beta-lactam agents is often mediated by production of one or more types of beta-lactamases that hydrolyze the beta-lactam ring, inactivating the ... | 2003 | 12533457 |
| coordination geometries of metal ions in d- or l-captopril-inhibited metallo-beta-lactamases. | d- and l-captopril are competitive inhibitors of metallo-beta-lactamases. for the enzymes from bacillus cereus (bcii) and aeromonas hydrophila (cpha), we found that the mononuclear enzymes are the favored targets for inhibition. by combining results from extended x-ray absorption fine structure, perturbed angular correlation of gamma-rays spectroscopy, and a study of metal ion binding, we derived that for cd(ii)1-bcii, the thiolate sulfur of d-captopril binds to the metal ion located at the site ... | 2003 | 12668674 |
| methionine regeneration and aminotransferases in bacillus subtilis, bacillus cereus, and bacillus anthracis. | the conversion of ketomethiobutyrate to methionine has been previously examined in a number of organisms, wherein the aminotransferases responsible for the reaction have been found to be members of the ia subfamily (l. c. berger, j. wilson, p. wood, and b. j. berger, j. bacteriol. 183:4421-4434, 2001). the genome of bacillus subtilis has been found to contain no subfamily ia aminotransferase sequences. instead, the analogous enzymes in b. subtilis were found to be members of the if subfamily. th ... | 2003 | 12670965 |
| probiotic and milk technological properties of lactobacillus brevis. | two lactobacillus brevis strains atcc 8287 and atcc 14869(t), were evaluated for their applicability as putative probiotics in dairy products. the strains expressed good in vitro adherence to human caco-2 and intestine 407 cells and tolerated well low ph, bile acids and pancreatic fluid under in vitro conditions. in antimicrobial activity assays, strain atcc 8287 showed inhibitory properties toward selected potential harmful microorganisms, particularly against bacillus cereus. both l. brevis st ... | 2003 | 12672593 |
| rope-producing strains of bacillus spp. from wheat bread and strategy for their control by lactic acid bacteria. | two types of white wheat bread (high- and low-type loaves) were investigated for rope spoilage. thirty of the 56 breads tested developed rope spoilage within 5 days; the high-type loaves were affected by rope spoilage more than the low-type loaves. sixty-one bacillus strains were isolated from ropy breads and were characterized on the basis of their phenotypic and genotypic traits. all of the isolates were identified as bacillus subtilis by biochemical tests, but molecular assays (randomly ampli ... | 2003 | 12676716 |
| production of diarrheal enterotoxins and other potential virulence factors by veterinary isolates of bacillus species associated with nongastrointestinal infections. | with the exceptions of bacillus cereus and bacillus anthracis, bacillus species are generally perceived to be inconsequential. however, the relevance of other bacillus species as food poisoning organisms and etiological agents in nongastrointestinal infections is being increasingly recognized. eleven bacillus species isolated from veterinary samples associated with severe nongastrointestinal infections were assessed for the presence and expression of diarrheagenic enterotoxins and other potentia ... | 2003 | 12676723 |
| detection of anthrax simulants with microcalorimetric spectroscopy: bacillus subtilis and bacillus cereus spores. | recent advances in the development of ultrasensitive micromechnical thermal detectors have led to the advent of novel subfemtojoule microcalorimetric spectoscopy (calspec). on the basis of principles of photothermal ir spectroscopy combined with efficient thermomechanical transduction, calspec provides acquisition of vibrational spectra of microscopic samples and absorbates. we use calspec as a method of identifying nanogram quantities of biological micro-organisms. our studies focus on bacillus ... | 2003 | 12683752 |
| demonstration of a hermetic airborne ozone disinfection system: studies on e. coli. | an enclosed flow-through system using airborne ozone for disinfection and which removes the ozone with a catalytic converter was tested with a strain of escherichia coli. petri dishes containing the microorganisms were inserted in a chamber and exposed for 10-480 min to ozone concentrations between 4 and 20 ppm. death rates in excess of 99.99% were achieved. survival data is fitted to a two-stage curve with a shoulder based on the multihit target model. ozone was removed from the exhaust air to ... | 2003 | 12688846 |
| inactivation of bacillus cereus spores by high hydrostatic pressure at different temperatures. | the effect of ph on the initiation of germination and on the inactivation of bacillus cereus (kctc 1012) spores during high hydrostatic pressure processing (hpp) with pressures of 0.1 to 600 mpa at different temperatures was investigated. two different high-pressure treatments were adopted to evaluate the effect of ph on the inactivation of b. cereus on sporulation medium and in suspension medium. inactivation of b. cereus spores with hpp treatment was affected more by sporulation medium ph than ... | 2003 | 12696682 |
| a retail and consumer phase model for exposure assessment of bacillus cereus. | an exposure assessment is conducted for psychrotrophic and mesophilic bacillus cereus in a cooked chilled vegetable product. a model is constructed that covers the retail and consumer phase of the food pathway, using the output of a similar model on the industrial process as input. microbial growth is the predominant process in the model. variability in time and temperature during transport and storage is included in the model and different domestic refrigerator temperature distributions are com ... | 2003 | 12706041 |
| demonstration of labeless detection of food pathogens using electrochemical redox probe and screen printed gold electrodes. | the demonstration of a labeless immunosensor for the detection of pathogenic bacteria using screen printed gold electrodes (spges) and a potassium hexacyanoferrate(ii) redox probe is reported. gold electrodes were produced using screen printing and the gold surfaces were modified by a thiol based self assembled monolayer (sam) to facilitate antibody immobilisation. sams based on the use of thioctic acid (ta), mercaptopropionic acid (mpa) and mercaptoundecanoic acid (mua) were evaluated. followin ... | 2003 | 12713911 |
| ym-266183 and ym-266184, novel thiopeptide antibiotics produced by bacillus cereus isolated from a marine sponge. i. taxonomy, fermentation, isolation, physico-chemical properties and biological properties. | novel antibiotics, ym-266183 (1) and ym-266184 (2), were found in the culture broth of bacillus cereus qn03323 which was isolated from the marine sponge halichondria japonica. the structures of both antibiotics were determined by several spectroscopic experiments as new thiopeptide compounds. they exhibited potent antibacterial activities against staphylococci and enterococci including multiple drug resistant strains, whereas they were inactive against gram-negative bacteria. | 2003 | 12715871 |
| ym-266183 and ym-266184, novel thiopeptide antibiotics produced by bacillus cereus isolated from a marine sponge ii. structure elucidation. | ym-266183 and ym-266184 are new antibacterial substances that have activity against drug-resistant bacteria produced by bacillus cereus qn03323. these structures were elucidated by ms and nmr spectral analysis. ym-266183 and ym-266184 are the cyclic thiopeptides containing thiazole and pyridine moieties, and several unusual amino acids. | 2003 | 12715872 |
| allosteric interactions within subsites of a monomeric enzyme: kinetics of fluorogenic substrates of pi-specific phospholipase c. | two novel water-soluble fluorescein myo-inositol phosphate (flip) substrates, butyl-flip and methyl-flip, were used to examine the kinetics and subsite interactions of bacillus cereus phosphatidylinositol-specific phospholipase c. butyl-flip exhibited sigmoidal kinetics when initial rates are plotted versus substrate concentration. the data fit a hill coefficient of 1.2-1.5, suggesting an allosteric interaction between two sites. two substrate molecules bind to this enzyme, one at the active sit ... | 2003 | 12719256 |
| genomics: relative pathogenic values. | 2003 | 12721608 | |
| the genome sequence of bacillus anthracis ames and comparison to closely related bacteria. | bacillus anthracis is an endospore-forming bacterium that causes inhalational anthrax. key virulence genes are found on plasmids (extra-chromosomal, circular, double-stranded dna molecules) pxo1 (ref. 2) and pxo2 (ref. 3). to identify additional genes that might contribute to virulence, we analysed the complete sequence of the chromosome of b. anthracis ames (about 5.23 megabases). we found several chromosomally encoded proteins that may contribute to pathogenicity--including haemolysins, phosph ... | 2003 | 12721629 |
| genome sequence of bacillus cereus and comparative analysis with bacillus anthracis. | bacillus cereus is an opportunistic pathogen causing food poisoning manifested by diarrhoeal or emetic syndromes. it is closely related to the animal and human pathogen bacillus anthracis and the insect pathogen bacillus thuringiensis, the former being used as a biological weapon and the latter as a pesticide. b. anthracis and b. thuringiensis are readily distinguished from b. cereus by the presence of plasmid-borne specific toxins (b. anthracis and b. thuringiensis) and capsule (b. anthracis). ... | 2003 | 12721630 |
| regioselective glucosylation of pyridoxine by microorganisms. | microorganisms from culture collections and isolates from nature were screened for the ability to catalyze the regioselective glucosylation of pyridoxine (pn) to produce pyridoxine 5'-alpha-d-glucoside (pn-5'-alpha-g) or pyridoxine 4'-alpha-d-glucoside (pn-4'-alpha-g). transglucosylation activity specific to 5'-position of pn was found in fungi belonging to genera such as coriolus and verticillium, and activity at the 4'-position of pn was found in bacteria belonging to genera such as bacillus a ... | 2003 | 12723596 |
| the inhibitor thiomandelic acid binds to both metal ions in metallo-beta-lactamase and induces positive cooperativity in metal binding. | thiomandelic acid is a simple, broad spectrum, and reasonably potent inhibitor of metallo-beta-lactamases, enzymes that mediate resistance to beta-lactam antibiotics. we report studies by nmr and perturbed angular correlation (pac) spectroscopy of the mode of binding of the r and s enantiomers of thiomandelic acid, focusing on their interaction with the two metal ions in cadmium-substituted bacillus cereus metallo-beta-lactamase. the 113cd resonances are specifically assigned to the metals in th ... | 2003 | 12724330 |
| genome differences that distinguish bacillus anthracis from bacillus cereus and bacillus thuringiensis. | the three species of the group 1 bacilli, bacillus anthracis, b. cereus, and b. thuringiensis, are genetically very closely related. all inhabit soil habitats but exhibit different phenotypes. b. anthracis is the causative agent of anthrax and is phylogenetically monomorphic, while b. cereus and b. thuringiensis are genetically more diverse. an amplified fragment length polymorphism analysis described here demonstrates genetic diversity among a collection of non-anthrax-causing bacillus species, ... | 2003 | 12732546 |
| a mammalian cell regulatory agent, ceres-18, inhibits yeast cell proliferation but not bacterial replication. | a cell regulatory sialoglycopeptide, ceres-18, purified from intact bovine cerebral cortex cells, has exhibited the capability of reversibly inhibiting cellular dna synthesis and the proliferation of a wide array of mammalian cells. in the present study, the effect of ceres-18 on the proliferation of bacterial ( bacillus cereus and escherichia coli) and yeast ( saccharomyces cerevisiae and schizosaccharomyces pombe) cells was investigated. the results showed that replication and viability of the ... | 2003 | 12732963 |