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recovery of viruses and bacteria in waters off bondi beach: a pilot study.a pilot study was conducted between february and april, 1989, on the occurrence of sewage-derived viruses and bacteria in the beach and nearshore waters off bondi, sydney. enteroviruses were isolated from 41% of a total of 66 sewage, sea-water, grease and sediment samples. poliovirus vaccine strains accounted for 78% of the isolates. adenoviruses were isolated four times and coxsackievirus b was isolated twice in samples that were collected away from the bathing area. rotavirus and hepatitis a v ...19892556634
aeromonas species in aquaria: a reservoir of gastrointestinal infections?to assess the role of aquaria in the epidemiology of aeromonas associated gastroenteritis, the prevalence and antibiotic susceptibility of aeromonads in ornamental aquaria were determined and compared to that of isolates obtained from patients with gastroenteritis. thirty-eight (76%) of 50 aquaria, including those located in our hospital, yielded 61 isolates: 24 (39.3%) aeromonas sorbria, 18 (29.0%) a. hydrophila, 15 (24.6%) a. caviae, and four (6.5%) which could not be speciated. in comparison, ...19892567310
oral cholera vaccines containing b-subunit-killed whole cells and killed whole cells only. ii. field evaluation of cross-protection against other members of the vibrionaceae family.because of demonstrable cross-reactivity of cellular antigens contained in b subunit-killed whole-cell (bs-wc) and killed whole-cell-only (wc) oral cholera vaccines with antigens of various non-cholera species of the family vibrionaceae (ncv), the protection conferred by the vaccines against diarrhoea associated with ncv was evaluated during a randomized, double-blind field trial in bangladesh. children aged 2-15 years and women aged greater than 15 years (62,285 in number) received three doses ...19892665350
[significance of the occurrence of aeromonas of various biotypes in the third world].in investigations carried out in the refugee camps of the polisario (south algeria), in the neighbourhood of the inhabitants of the waste disposal sites of cairo and in the ayoun el atrous and nema (mauritania) regions also in the bida and minna hospitals. aeromonas hydrophila and aeromonas spec. were repeatedly found in the investigated material. therefore, it was of particular interest for us to investigate this type of organism.19892641444
motile aeromonas infection of striped (grey) mullet mugil cephalus.infection of striped mullet (mugil cephalus) with aeromonas hydrophila results in an acute septicemic disease. the disease can be experimentally induced by intramuscular injection, skin or gill scarification or by the oral route using pellets purposely seeded with bacteria. the organism was isolated from the blood 1-2 days after infection and from all organs 24 hr or longer after infection. the disease is characterized by early inflammatory and proliferative changes and later necrotic changes. e ...19892575883
purification and characterization of aeromonas hydrophila beta-hemolysin.aeromonas hydrophila hemolysin was excreted in our culture conditions during the stationary growth phase. the toxin was purified to homogeneity by a three-step method: ultrafiltration, acid precipitation in the presence of rna and anion exchange chromatography with fplc apparatus. beta-hemolysin is a protein not associated with lipids, carbohydrates or nucleic acids whose subunit mol. wt is 51,000. the mol. wt determined by polyacrylamide gel electrophoresis suggests that the molecule is in a tr ...19892516922
[water studies of inhabitants of refuse disposal sites in cairo. 1].in two waste disposal sites in cairo, drinking water samples from springs, drinking vessels and kitchens were investigated. total germ count lay seldom below one thousand and ten million pro ml. apart from various faecal germs, aeromonas hydrophila was also found. the high occurrence of diarrhoeal diseases among the inhabitants of the waste disposal sites can be traced with certainty to the extremely bad drinking water quality.19892641445
[antibacterial and bactericidal activities of japanese green tea].we found that extracts of japanese green tea leaves inhibited the growth of various bacteria causing diarrheal diseases. all tea samples tested showed antibacterial activity against staphylococcus aureus, s. epidermidis, vibrio cholerae o1, v. cholerae non o1. v. parahaemolyticus, v. mimicus, campylobacter jejuni and plesiomonas shigelloides. none of the tea samples had any effect on the growth of v. fluvialis, aeromonas sobria, a. hydrophila, pseudomonas aeruginosa, salmonella enteritidis, ente ...19892677434
incidence of aeromonads in samples from an abattoir processing lambs.two hundred and thirty three samples of lamb carcasses, livers, kidneys and faeces, collected at a local abattoir, were examined to determine the incidence of motile aeromonas spp. wash water from the abattoir was also tested. direct plating on starch ampicillin agar and enrichment in alkaline peptone water were used. the incidence of aeromonads was low. they were detected only after enrichment in 5/47 faecal samples and 11/50 carcass samples. the 41 strains of aeromonas isolated were identified ...19892613594
aeromonas hydrophila strains with moderate virulence.a group of aeromonas hydrophila strains was obtained from very different origins which showed very similar characteristics (lipopolysaccharide, outer-membrane protein profile, toxin production, bacteriophage sensitivity and moderate virulence), besides the heterogenicity observed among the a. hydrophila strains. this group of a. hydrophila strains with moderate virulence can be easily recognized by their bacteriophage sensitivity pattern.19892593869
microbiological and chemical analysis of drinking water in southern india (with special consideration of aeromonas species).analyses of drinking water taken from central and individual water supplies in southern india (idukki/kerala) showed bacterial counts of 10(3)-10(6). e. coli were found in 11% and 28% of the samples. aeromonads were identified more frequently than e. coli at 44% in central and 38% in individual water supplies. due to this high rate of contamination, aeromonas spp. must be considered as a possible cause of diarrhoea and other infections in this area.19892684763
[studies on bacterial agents in acute diarrheal disease (1985-1987)].a three-year epidemiological study (from january 1985 to december 1987) was carried out on sporadic cases of acute diarrhea. a total of 2889 fecal specimens in cary-blair transport medium were examined for bacterial enteric pathogens, and 832 strains of fifteen species were isolated from 739 specimens, 73 patients having two or more pathogens. c. jejuni shared 51.7%, salmonella spp. 18.3%, v. parahaemolyticus 10.3%, and aeromonas spp. 15.7% of total fecal specimens. isolation rates of c. jejuni ...19892614097
aeromonas endocarditis in a patient with chronic hepatitis-b infection.aeromonas, a genus of gram-negative bacteria normally found in water and soil, is well established as a pathogen in the animal kingdom. often considered as a pathogen of low virulence, its role in human infections has recently been recognised. aeromonas infections in humans range from cellulitis to septicaemia. endocarditis is rare. we describe here a patient with a chronic liver disease with aeromonas bacteremia and endocarditis.19892617304
haemagglutinating activity of aeromonas spp. from different sources; attempted use as a typing system.the haemagglutinating ability of 141 isolates of aeromonas spp. for human, horse and guinea-pig erythrocytes was examined. although the majority of isolates (136/141) agglutinated human group o erythrocytes, all the eight possible patterns of agglutination were observed. haemagglutination of human group o erythrocytes, but not horse or guinea-pig, was associated with the ability to agglutinate yeast cells (saccharomyces) and with aggregation in a low concentration of ammonium sulphate. haemagglu ...19892649385
mixed infections in childhood diarrhoea: results of a community study in kiambu district, kenya.one thousand four hundred and twenty diarrhoea specimens from 846 children aged 0 to 60 months were collected and analysed for bacteria, parasites and rotavirus over a 16 month period, from june 1985 to september 1986 inclusive. the study was conducted in 4 villages situated in kiambu district, kenya. all the specimens were analysed for rotavirus and parasites, including cryptosporidium. the majority of the specimens were analysed for enteropathogenic escherichia coli (epec), shigella, salmonell ...19892606013
some observations on the faecal carriage of mesophilic aeromonas species in cows and pigs.replicate faecal samples from healthy individual pigs and cows were examined for the presence of aeromonas sp. over a 12-month period. aeromonads were found to be minor components of the faecal flora, only 8.8% of 520 samples from pigs and 4.6% of 481 samples from cows proving positive. isolation rates in both groups of animals were seasonal. a hydrophila (62% of the isolates) was the predominant species in cows, followed by a. caviae (32%) and a. sobria (15%). this pattern was also recorded in ...19892606161
bacterial and viral causes of acute diarrhoea in children in kuwait.bacterial and viral causes of acute diarrhoea were studied prospectively for one year in 343 hospitalised young children in kuwait. in 288 (84%) patients, one or more pathogens were identified compared with 12 of 86 (13.9%) children admitted with diseases other than diarrhoea (p less than 0.01). forty-four (12.9%) of the patients were infected with two or more pathogens. viral agents detected in the stools were rotaviruses (40.2%), enteric adenoviruses (1.7%), and enteroviruses (1.5%). enterobac ...19892632640
[the occurrence of escherichia coli, aeromonas hydrophila, plesiomonas shigelloides and clostridium perfringens in the intestinal flora of gray herons (ardea cinerea)].the flora of the large intestine of 92 grey herons was examined for the frequency of aerobic and microaerobic growing bacteria. clostridium perfringens, aeromonas hydrophila, plesiomonas shigelloides and e. coli were isolated from 55%, 48%, 14% and 35% of the birds, respectively. it could be demonstrated that the findings of these bacteria in the intestinal flora are depending on the age of the birds. the percentage of carriers of clostridium perfringens, aeromonas hydrophila and plesiomonas shi ...19892548355
aeromonas spp. in foods: a significant cause of food poisoning?from a total of 563 samples of various foodstuffs purchased from retail outlets in the reading area 287 were found to contain mesophilic aeromonas spp. the types of sample which were most frequently contaminated were poultry (79.3%) and offal (84.3%). of three media compared for their efficiency in recovering aeromonas spp. after enrichment in alkaline peptone water, difco aeromonas was the most efficient. cytotoxin was produced by approximately 50% of the a. hydrophila and a. sobria strains but ...19892641273
isolation of medically significant vibrio species from riverine sources in south east queensland.vibrio and vibrio-like bacteria were isolated from water, sediments, plants and faeces from eight riverine sites in south east queensland, australia, using filtrations, enrichments and selective growth on thiosulphate-citrate-bile salts-sucrose (tcbs) and simidu agars. isolates (402 in toto) were classified by numerical analysis of phenotypic characteristics and comparison with fifteen reference cultures. of the isolates 41 (10.2%) were identified as vibrio cholerae, 33 (8.2%) as v. fluvialis an ...19892607994
[acute gastroenteritis due to vibrio parahaemolyticus in spain. presentation of 8 cases].eight cases of acute gastroenteritis caused by vibrio parahaemolyticus in humans are described; to our knowledge, they are the first such reported cases in spain. all cases appeared between august 20th and october 15th, with a frequency of 8.3% regarding the overall adult patients with acute gastroenteritis, and 11.5% regarding the overall patients with positive stool culture for any enteropathogenic organism. the eight strains were kanagawa positive and in three patients other enteropathogenic ...19892716372
[are aeromonas species a cause of diarrheal diseases in yugoslavia?].isolation and identification of aeromonas spp. have been performed in 1,610 stools in the period from january 1987 to april 1988. of 1300 stool specimens taken from the patients with diarrheal syndrome (540 stools of children and 760 stools of adults) aeromonas spp. were isolated only in 4 patients (0.30%, p less than 0.001). positive stools were found in 3 adult patients and one child. in 310 examined stools of healthy adult persons aeromonas spp. were not isolated. a significantly higher incid ...19892609506
bacterial growth and toxin production in ileostomy effluents.escherichia coli (2), vibrio cholerae (2) and aeromonas sobria (1) strains were examined for their ability to grow and produce toxins in samples of ileostomy fluid. three categories of response were observed: no detectable growth, growth without detectable toxin, and growth with detectable toxin. clear differences were apparent between samples of ileostomy fluid obtained from different individuals and between samples obtained from the same individual at different times. the patterns of response ...19892649680
suicide phenomenon in mesophilic aeromonads as a basis for species identification.the acetic acid-mediated suicide phenomenon in mesophilic aeromonads in conjunction with tests for aerogenicity and esculin hydrolysis served as the basis for species identification. of 210 aeromonas isolates tested at 30 degrees c, 88 were identified as aeromonas hydrophila (nonsuicidal, aerogenic, esculin positive), 52 were identified as a. sobria (suicide variable, aerogenic, esculin negative), and 70 were identified as a. caviae (suicidal, anaerogenic, esculin positive). these identification ...19892723039
[production of antibiotic substances by natural variants of the marine bacterium vibrio fischeri].it was shown that under definite conditions there was competition between natural variants of sea bacteria belonging to v. fischeri. natural variants of v. fischeri, strain 6 differed in their resistance to streptomycin and had different growth rates under conditions of limited aeration. morphologically all the variants were identical. v. fischeri p-0, v. fischeri p-1 and v. fischeri p-2 were studied. the study revealed that v. fischeri p-0 produced a non-dialysing thermostable trypsin-sensitive ...19892730220
characterization of aeromonas salmonicida mutants with low-level resistance to multiple antibiotics.aeromonas salmonicida mutants selected for low-level resistance to small-molecular-mass antibiotics occur at frequencies that suggest point mutations and exhibit pleiotropic effects such as a multiple low-level antibiotic resistance, changes in outer membrane protein profiles, and loss of major exoprotease activity. multiple low-level resistance appeared as the result of decreased outer membrane permeability associated with a change from a 38.5- to a 37-kilodalton (kda) outer membrane protein. t ...19892653212
enteroaggregative escherichia coli associated with persistent diarrhea in a cohort of rural children in india.a cohort of 452 rural children was followed longitudinally for 13 mo to ascertain the role of hep-2 cell adherent escherichia coli and other pathogens in causing acute (less than or equal to 14 d) and persistent (greater than 14 d) diarrhea. aeromonas, campylobacter jejuni, e. coli manifesting localized adherence to hep-2 cells and enterotoxigenic e. coli were significantly associated with acute diarrhea. e. coli strains that exhibit aggregative adherence, so-called enteroaggregative e. coli, a ...19892656875
[microbiologic study of low quality raw material obtained from pacific salmon].the authors examined the microflora in the regions occupied with food fish and food manufacture from pacific salmon in order to establish its interrelations with other characteristics of the quality. with this purpose in view sea and river water in the places of catching, freshly catched salmon and salted food were studied for the presence of putrefactive bacteria, potentially pathogenic for hydrobionts and man. the data obtained have demonstrated that the main causes of the deterioration of the ...19892660405
bacterial action of fertilization envelope extract from eggs of the fish cyprinus carpio and plecoglossus altivelis.the vitelline envelope (ve) and fertilization envelope (fe) in eggs of the fish cyprinus carpio and plecoglossus altivelis were purified by homogenization of eggs or embryos in 5 mm tris-hcl buffer, ph 7.0, containing 2 mm ethylenediamine tetraacetic acid disodium salt (edta), except for processing of ves in plecoglossus eggs, and by repeated washing wih the same buffer. to extract the outermost layer material, the purified ves and fes were processed overnight at 4 degrees c in 5 mm tris-hcl buf ...19892661718
[evaluation of a new semi-automatic method of identifying enterobacteriaceae, m.i.s.-enterobacteriaceae].m.i.s.-enterobacteriaceae is a new kit for identifying enterobacteriaceae using a microplate consisting of 21 biochemical characters with automated reading and interpretation. the validity of this method was studied by the identification of 350 strains of enterobacteria belonging to 44 species and comparison with the classical method of identification in test-tubes. results showed a diagnosis accuracy of 96 p. cent at the species level and 97.7 p. cent at the genus level. diagnosis accuracy reac ...19892665580
effect of temperature on hemolysin production in aeromonas spp. isolated from warm and cold environments.the effect of growth temperature on the hemolysin production in 97 aeromonas isolates was studied. only 9% of the isolates obtained from low-temperature sources such as refrigerated foods, drinking water and trout aquaculture were able to produce high hemolysin titers (titer greater than 128) at 37 degrees c compared with 65% of the isolates from warm sources such as human clinical cases and warm water aquaculture (p less than 0.001). in contrast, 61% of the low-temperature isolates were produci ...19892641493
aeromonas as a human pathogen.although the first aeromonas strain was described by zimmermann as early as in 1890, it took 60 years until caselitz established human pathogenicity of strains then called "vibrio jamaicensis". since then, and especially in the last 10 years, there have been increasing numbers of reports on different infections caused by members of the genus aeromonas. these include sepsis; meningitis; cellulitis; necrotizing fasciitis; ecthyma gangrenosum; pneumonia; peritonitis; conjunctivitis; corneal ulcer; ...19892649316
rapid method to detect shiga toxin and shiga-like toxin i based on binding to globotriosyl ceramide (gb3), their natural receptor.shiga toxin and the closely related shiga-like toxins produced by escherichia coli represent a group of very similar cytotoxins that may play an important role in diarrheal disease and hemolytic uremic syndrome. these toxins have the same biologic activities and according to recent studies also share the same binding receptor, globotriosyl ceramide (gb3). they are currently detected, on the basis of their ability to damage several cell lines, by using expensive and tedious assays that require fa ...19892666433
etiology of childhood diarrhea in korea.to assess the role of recently recognized enteropathogens in childhood diarrhea in korea, 231 children with diarrhea admitted to and 104 children without diarrhea seen at the well-baby clinic or the outpatient department of hanyang university hospital in seoul, korea, were evaluated during a 14-month period. stools were cultured for bacterial pathogens, including enterotoxigenic (heat-labile and heat-stable enterotoxin-producing) and enteroadherent organisms. only those stools obtained from pati ...19892666437
toxin production by strains of aeromonas hydrophila grown in laboratory media and prawn purée.toxin production by four strains of aeromonas hydrophila grown at 30 and 37 degrees c in two laboratory media and prawn purée was studied. three different cell lines were used to test for cytotoxic activity, haemolytic activity was tested against rabbit and guinea pig erythrocytes, proteolytic activity was assayed with azo-casein and enterotoxic activity using the suckling mouse assay. results showed reduced cytotoxic and haemolytic activities in prawn purée compared with the two media, but in m ...19892641490
purification and partial characterization of a cytotonic enterotoxin produced by aeromonas hydrophila.this report describes the purification and partial characterization of a cytotonic enterotoxin produced by a human diarrheal isolate (ssu) of aeromonas hydrophila. the extracellular enterotoxin was purified by (nh4)2so4 precipitation, hydrophobic column chromatography, and chromatofocusing. the highly purified enterotoxin exhibited a molecular mass of 44 kda and an isoelectric point in the range of 4.3 - 5.5 as determined by chromatofocusing. western blot analysis using aeromonas anti-enterotoxi ...19892766121
exclusion of clinically atypical or microbiologically mixed diarrhoeal episodes from outcome events in a field trial of oral cholera vaccines.we investigated whether alternative clinical and microbiological criteria for outcome events affected estimates of vaccine efficacy in a randomized, double-blind field trial of b subunit-killed whole cell (bs-wc) and killed whole cell-only (wc) oral cholera vaccines among 62,285 rural bangladeshi participants. at one year of follow-up estimates of vaccine protective efficacy (pe = 60%, p less than 0.0001 for bs-wc; pe = 54%, p less than 0.0001 for wc) against all treated diarrhoeal episodes asso ...19892767860
characterization of aeromonas schubertii strains recently isolated from traumatic wound infections.recent studies have resulted in the proposal of a new species, aeromonas schubertii (mannitol, sucrose, and indole negative), formerly termed enteric group 501, on the basis of the study of seven strains isolated from the southeastern and southwestern united states and puerto rico. we have isolated two phenotypically similar a. schubertii strains from infected human wounds sustained in the chesapeake bay area. their identification was confirmed by dna-dna hybridization to the centers for disease ...19892768470
bacterial diseases of marine fish.the principal bacterial diseases found among wild and cultured marine fish are reviewed. the bacterial agents discussed include the gram-negative pathogens in the vibrio, aeromonas, pasteurella and edwardsiella genera, renibacterium salmoninarum and the myxobacteria, streptococci, mycobacteria, nocardias and anaerobic organisms which have been associated with fish diseases. methods for the isolation and identification of these organisms are described.19892678720
clinical and epidemiologic aspects of members of aeromonas dna hybridization groups isolated from human feces.between june 1982 and may 1987 aeromonas species were isolated from 208 of 34,311 (0.61%) fecal samples submitted to a regional public health laboratory in the netherlands. aeromonas isolates were found most frequently in summer and rarely in winter. of 169 aermonas isolates that were available for further study, 19% were isolated from patients with a mixed infection, 5% from patients with underlying diseases, and 15% from patients who used medication that could predispose the intestinal tract t ...19892768441
ultrastructural study of the response of eosinophil granule cells to aeromonas salmonicida extracellular products and histamine liberators in rainbow trout salmo gairdneri richardson.intraperitoneal injections of aeromonas salmonicida extracellular products (ecp), compound 48/80 and concanavalin a were found to degranulate the eosinophil granule cells (egc) in the lower intestine and rectum of the rainbow trout salmo gairdneri richardson. ultrastructurally, the egc response resembled the anaphylactic granule extrusion of mammalian mast cells. varying degrees of granule vacuolation and loss of electron density occurred. labyrinthine channels were observed at the peak of degra ...19892776934
isolation of aeromonas spp. from water by using anaerobic incubation.a membrane filtration method incorporating a combination of anaerobic and aerobic incubation has been developed for the enumeration of aeromonas spp. in drinking water. the use of anaerobic incubation improved the detection of aeromonas spp. by reducing the growth of nonaeromonads. the confirmation rate of presumptive aeromonas spp. identified on the initial isolation agar exceeded 92%.19892679380
volatile compounds associated with microbial growth on normal and high ph beef stored at chill temperatures.volatile compounds produced by pseudomonas fragi and mixed, natural floras on beef of normal ph (5.5-5.8; glucose greater than 1500 micrograms/g) and high ph (6.3-6.8; glucose less than 10 micrograms/g) included a range of alkyl esters and a number of sulphur-containing compounds including dimethylsulphide but not hydrogen sulphide. production of the last was a property common to the other gram-negative organisms tested viz. hafnia alvei, enterobacter agglomerans, serratia liquefaciens, alteromo ...19892753835
the quantitative relationship of lethality between extracellular protease and extracellular haemolysin of aeromonas salmonicida in atlantic salmon (salmo salar l.).an additive relationship of lethality between purified protease and haemolysin of the extracellular products (ecp) of aeromonas salmonicida was demonstrated by i.p. injection in atlantic salmon (salmo salar l.). the lethal toxicity of the combinations of protease and haemolysin follow a linear regression line y = -54.54x + 2400. the ld50 of protease and haemolysin when injected separately was 2400 ng/g fish and 44 ng protein/g fish, respectively.19892689276
[the ratios and kinds of clinical bacteria isolated in taiwan's large-size hospitals].the prevalence of clinical bacteria, as isolated from linko chang-gung memorial hospital (2,300 beds) in the period january 1985 to december 1986 and from taipei veterans general hospital (2,300 beds) during the period january 1986 to december 1986, was analyzed with the following findings: (i) the isolation ratio of anaerobic and aerobic or facultative bactria during the period of investigation were 7.8% (5,513/70,799) and 92.2% (65,286/70,799), respectively. (ii) of the total aerobic or facult ...19892791722
aeromonas hydrophila upper extremity infection.a severe soft tissue infection of the upper extremity caused by aeromonas hydrophila followed a water skiing injury in which a tow rope caused degloving of a portion of the skin and severe contusion to underlying muscle. infection was established within 36 hours of the injury, accompanied by fever, leukocytosis, and a foul odor. rapid clinical improvement occurred following radical debridement of all nonviable tissue and antibiotic therapy. in cases involving water contamination, aeromonas hydro ...19892754206
[aeromonas-associated diarrhea]. 19892692836
[studies on motile-aeromonas infection: incidence of motile-aeromonas in river mud, river water and fresh-water fish].during the period from october 1982 to july 1984, a total of 1,157 specimens that consisted of 132 river and lake water, 514 river and lake muds, and 511 fresh-water fish caught in both tama river and sagami river were examined the presence of the organisms. of them, 132 (100%) river and lake waters, 304 (59.1%) river and lake muds, and 462 (90.4%) intestinal contents of fresh-water fish were found to have harbor a mean concentration of 1.3 x 10(3)/l, 1.6 x 10(6)/g, and 1.1 x 10(6)/g of motile-a ...19892693540
improved efficiency m13 cloning using electroporation. 19892798147
comparison of three assays to detect enterotoxigenicity of aeromonas isolates from patients with diarrhoea in vellore, south india.fifty strains of aeromonas spp, isolated from patients with diarrhoea, were tested by three different assays to detect their enterotoxigenicity. culture filtrates of the isolates were tested for their ability to produce haemolysin and to produce cytopathic changes in vero cells and for their effects in the suckling mouse assay. the data indicated that biotypes alone are not adequate indicators of pathogenicity of different groups of aeromonas isolated from patients with diarrhoea. no significant ...19892698900
[application of the moore swab method to the isolation of aeromonas spp. from residual waters].the moore swab technique was applied in waste water from three different areas of the "pedro kouri" institute of tropical medicine, with the view to isolate organisms of the genus aeromonas. swabs were placed once a week, in the wards of medicine, screening, and diagnostic laboratory. of 13 swabs placed in the medicine ward, 8 were positive. of 15 swabs placed in diagnostic laboratory, 8 were positive, and of 18 placed in the screening ward, 12 were positive. of a total 46 swabs, 28 disclosed or ...19892701185
stimulation of specific aeromonas antibody secretion in rat intestine by gamma-aminobutyric acid.this study was undertaken to assess a possible role for gamma-aminobutyric acid (gaba) in the regulation of intestinal secretion of iga and igg antibodies. rats were immunized with culture supernatant of aeromonas hydrophila isolate ssu. this culture supernatant contains a number of toxins that may be considered virulence factors. after 24 days of immunization, rats were anesthetized and a 10-cm intestinal segment was intubated and ligated at both ends in situ. the intestinal loop was perfused w ...19892702891
associated bacterial flora, growth, and toxicity of cultured benthic dinoflagellates ostreopsis lenticularis and gambierdiscus toxicus.the growth, toxicity, and associated bacterial flora of 10 clonal cultures of the toxic benthic dinoflagellates ostreopsis lenticularis and gambierdiscus toxicus isolated from the coastal waters of southwest puerto rico have been examined. clonal cultures of o. lenticularis grew more rapidly and at broader temperature ranges than those of g. toxicus. all five ostreopsis clones were toxic, while only one of the five gambierdiscus clones was poisonous. the degree of toxicity among poisonous clones ...19892705766
chronic colitis after aeromonas infection.three patients with an acute colitis in which the only pathogen detected was either aeromonas hydrophila or a sobria progressed to a chronic phase after the infection had been eliminated by antibiotic treatment in two and had resolved spontaneously in the third. the final diagnosis in each case was ulcerative colitis. two of the patients have responded to anti-inflammatory medication but one has required panproctocolectomy. the sequence of symptoms and observations in these cases, as well as in ...19892731763
comparative in-vitro activity of meropenem on clinical isolates from the united kingdom.mics of meropenem were determined for a wide range of common bacteria of clinical importance. for enterobacteriaceae, aeromonas spp., haemophilus influenzae, branhamella catarrhalis, neisseria gonorrhoeae, gardnerella vaginalis, campylobacter coli/jejuni, beta-haemolytic streptococci and anaerobes other than clostridium difficile, mics were almost always within the range 0.002-0.5 mg/l. the activity of meropenem for these organisms was always greater than that of imipenem and piperacillin, and w ...19892808214
in vitro studies of tigemonam: a comparison of the minimum inhibitory and minimum bactericidal concentrations (mic vs mbc).the in vitro activity of tigemonam, a new oral monobactam, was studied with special attention to minimum inhibitory concentrations (mics) and minimum bactericidal concentrations (mbcs). against 250 clinical isolates, it inhibited 100% of escherichia sp., klebsiella sp., serratia sp., citrobacter sp., providencia sp., proteus sp., morganella sp., aeromonas sp., yersinia sp., shigella sp., and haemophilus sp. at 0.5 mg/l or less. with 1 mg/l, 75% of enterobacter sp. were inhibited; however, three ...19892809701
extracellular secretion of cloned aerolysin and phospholipase by aeromonas salmonicida.the promoterless structural genes for aerolysin and the extracellular phospholipase of aeromonas hydrophila were inserted into a multi-host-range expression vector and transferred into aeromonas salmonicida and escherichia coli. in both species, gene expression was under the control of the inducible tac promoter of the vector. neither the phospholipase nor the aerolysin was released by intact e. coli. instead, both proteins accumulated in the periplasm, leading to reduced growth and eventual cel ...19892708312
urinary tract infection by aeromonas hydrophila in a neonate. 19892737762
characterization and significance of aeromonas spp. isolated from diarrhoeic stools in nigeria.during a 12-month study period (january-december 1986), 12 aeromonas strains (eight a. hydrophila and four a. sobria) were isolated from the 400 diarrhoeic and 200 non-diarrhoeic stools examined. isolates were characterized for frequency of phenotypic properties associated with virulence using the suckling mouse test for enterotoxin assay; haemolysis of rabbit erythrocytes for haemolysin production, and haemagglutination of human group a cells (ha) for presence of colonization factor antigens (c ...19892709482
etiology of acute childhood diarrhoea in calcutta.of the 152 cases of acute diarrhoea, 124 (81.5%) revealed potential pathogens. altogether 27 (21.2%) out of 127 strains of escherichia coli, klebsiella pneumoniae, enterobacter, proteus and acinetobacter produced enterotoxin. single pathogenic bacteria (40 cases 26.3%), parasite (6; 6%), rota virus (6; 6%), toxigenic bacteria (19; 12.5%) and mixed agents (37; 24.24.3%) were recorded in 108 cases (71.0%). another 14 (9.2%) cases exclusively revealed moderate to heavy growth of suspected enteric p ...19892815324
[aeromonas hydrophila-related septicemia in the nile tilapia oreochromis niloticus].from diseased wild and cultured oreochromis niloticus in lower egypt, 17 aeromonas hydrophila isolates were recovered. the mortality was between 10% and 70% in among cultured fish. the course of the disease ran in an acute manner. for cultured fish, the disease outbreaks were found mainly in winter and for the wild nile fish, mortalities were observed in late spring and summer. additionally wild fish were affected with ectoparasites. the ld50 values of the isolates ranged between 10(3) and 10(7) ...19892719631
epidemiological significance of microbiological pollution criteria for river recreational waters.a retrospective follow-up study was conducted during the summer of 1986 in the french ardèche basin in order to assess the relationship between swimming-related morbidity and the bacteriological quality of the recreational water. 5737 tourists in eight holiday camps were questioned as to the occurrence of illness and their bathing habits during the week preceding the interviews. the rate-ratio contrasting swimmers and non-swimmers for total morbidity is 2.1 (1.8-2.4) = 95% confidence interval); ...19892722364
characterization of the aeromonas hydrophila group isolated from retail foods of animal origin.during a recent survey of retail fresh foods of animal origin (fish and seafood, raw milk, poultry, and red meats) for organisms of the aeromonas hydrophila group, we isolated representative strains from the various foods. in this study, we sought to characterize these isolates for biochemical properties and virulence-associated factors and to compare the food isolates with clinical isolates. we identified all food and clinical isolates as a. hydrophila and found that all isolates were typical i ...19892745695
oral cholera vaccines containing b-subunit-killed whole cells and killed whole cells only. i. cross-reacting antigens of members of family vibrionaceae and the vaccines.antigenic similarity between the oral cholera b subunit-whole cell (bs-wc) and whole-cell only (wc) vaccines and strains of the family vibrionaceae was studied by crossed immunoelectrophoresis. a reference system consisting of vibrio cholerae inaba e1 tor antigenic extract and homologous rabbit antiserum was applied in the study. the system was represented by 30 anodically migrating antigens forming distinct precipitation bands. antigenic extracts of other members of the family vibrionaceae show ...19892750268
fever response in laboratory-maintained and free-ranging chuckwallas (sauromalus obesus).the fever response was studied in laboratory-maintained and free-ranging chuckwallas. five animals were used in a self-pairing laboratory experiment in which each animal was first injected with a sterile, pyrogen-free saline solution. approximately 7 days later, each individual was injected with a solution of 4.75 x 10(9) aeromonas sobria (formerly a. hydrophila). saline-injected individuals had a mean preferred body temperature (mpbt) of 36.8 +/- 0.4 (se) degrees c, whereas bacteria-injected an ...19892750958
[the "diseased" or "dead" guillemots (uria aalge), three-toed gulls (rissa tridactyla), silver gulls (larus argentatus) and laughing gulls (larus ridibundus) found in the area of the german bay, 1982-1985].between 1982 and 1985 the cadavers of 50 guillemots (uria aalge), 41 kittiwakes (rissa tridactyla), 26 herring gulls (larus argentatus) and 34 black-headed gulls (larus ridibundus) were examined pathological, bacteriological and virological. the probable cause of death was established. parasitosis were particularly prevalent in herring gulls (49%), where the main infection--as in black-headed gulls--was with cestoides. in kittiwakes and guillemots mainly spiruroideae were recorded. the commonest ...19892752934
characterization of aeromonas sobria hemolysin by use of monoclonal antibodies against aeromonas hydrophila hemolysins.aeromonas sobria produces hemolysin in a form activable with trypsin under defined cultural conditions. in immunoblotting analyses with the culture supernatant of a. sobria, the monoclonal antibody reacting specifically to aeromonas hydrophila ca-11 hemolysin bound to the 53,000- and 49,000-dalton bands before and after trypsinization, respectively. the monoclonal antibody reacting to a. hydrophila ah-1 hemolysin did not bind either band. a. sobria hemolysin is, therefore, related antigenically ...19892768466
aeromonas hydrophila infection associated with the use of medicinal leeches.the use of medicinal leeches (hiruda medicinalis) is becoming more common after plastic surgery to control venous congestion of skin grafts. we describe a patient with aeromonas hydrophila infection whose graft was treated with medicinal leeches. the infection required systemic antibiotic therapy. a. hydrophila is the predominant bacterial flora in the gut of the leech, where it plays an essential role for the animal in the digestion of blood. the potential for a. hydrophila wound infection, and ...19892666448
growth of aeromonas spp. on butzler campylobacter selective agar and evaluation of the agar for the primary isolation of aeromonas spp. from clinical specimens.the fortuitous finding that aeromonas spp. grew well on butzler campylobacter selective medium (bcsa) in a microaerobic atmosphere at 42 degrees c prompted us to evaluate the performance of bcsa for selective isolation of aeromonads in comparison with ampicillin (30 micrograms/ml) sheep blood agar (asba30). overall recovery rates of aeromonads from 563 stool samples from patients with acute diarrhea were higher on asba30 (70.4%) than on bcsa (56.3%); however, 21 (29.5%) grew only on bcsa. the th ...19892915030
isolation of aeromonas hydrophila from a captive caracal lynx (felis caracal).aeromonas hydrophila was isolated from the internal organs of a captive caracal lynx (felis caracal) which died of acute septicemia. grossly, patchy areas of focal necrosis were found in the lungs, liver and kidney; there was ulceration in the stomach and intestines. microscopically, lesions contained cellular debris, neutrophils, lymphocytes and gram-negative bacilli. this is the first report of isolation of aeromonas hydrophila from a captive wild animal in nigeria.19892915393
bondage, dominance, irrigation, and aeromonas hydrophila: california dreamin'. 19892911156
phenotypic characterization and dna relatedness in human fecal isolates of aeromonas spp.phenotypic characteristics were used to identify 189 aeromonas strains isolated from human feces. one hundred forty-two of these strains were placed in 11 dna hybridization groups, and the genetic and phenotypic data were compared. according to the criteria of popoff, 66% of the strains were identified as aeromonas caviae, 18% were identified as a. sobria, and 16% were identified as a. hydrophila. some biochemical characteristics differed from the criteria of popoff; 19 of 40 (48%) of tested str ...19892913025
three-dimensional structure of an open form of the surface layer from the fish pathogen aeromonas salmonicida.cell-free culture supernatants of a lipopolysaccharide (lps) o-polysaccharide-deficient, single-insertion transposon mutant of the tetragonal surface protein array (s layer)-containing fish pathogen aeromonas salmonicida were examined by electron microscopy. negative staining showed that the s layer was released as sheets of tetragonal material, indicating that although surface retention of assembled s layer requires the presence of wild-type lps oligosaccharides, initial assembly of s-layer sub ...19892914846
synthesis of glycoconjugates derived from various lipopolysaccharides of the vibrionaceae family.conjugation of simple ketoses (such as 3-deoxy-d-manno-2-octulosonic acid and n-acetylneuraminic acid) and of various o-specific polysaccharides (from aeromonas hydrophila and aeromonas salmonicida) to the bifunctional spacer 1,6-hexanediamine, was achieved by reductive amination. the saccharide--1-(6-amino)-hexane alkyamines obtained were converted into the corresponding isothiocyanate derivatives and coupled to the free epsilon-amino group of lysine residues of the protein carrier bovine serum ...19892920731
purification and chemical characterization of a cholera toxin-cross-reactive cytolytic enterotoxin produced by a human isolate of aeromonas hydrophila.a bacterial protein toxin possessing hemolytic, enterotoxic, and cytotoxic activities as well as cross-reactivity to cholera toxin was purified from culture filtrates of a human diarrheal isolate of aeromonas hydrophila (ssu). this cytolytic enterotoxin was purified by ammonium sulfate precipitation, hydrophobic chromatography using phenyl-sepharose, anion-exchange chromatography on deae-bio-gel a, and size-exclusion high-performance liquid chromatography. the factor was a single polypeptide wit ...19892925244
bioactivity and immunological characterization of a cholera toxin-cross-reactive cytolytic enterotoxin from aeromonas hydrophila.a cytolytic enterotoxin of molecular weight 52,000 was isolated and purified from culture supernatants of a human diarrheal isolate (ssu) of aeromonas hydrophila. the toxin reacted with cholera antitoxin when tested in an enzyme-linked immunosorbent assay and by western blot (immunoblot) analysis. the appearance of cytotoxic and hemolytic activities in culture supernatant occurred simultaneously 8 h after the initial inoculation of the culture. loss of hemolytic activity and cholera toxin cross- ...19892925245
aeromonas hydrophila colitis presenting as medically refractory inflammatory bowel disease. 19892912037
the suicide phenomenon in motile aeromonads.certain strains of motile aeromonas species, including all those of aeromonas caviae examined, were shown to be suicidal. when they were grown in the presence of glucose at both 30 and 37 degrees c, there was rapid die-off of the organisms after 12 h of incubation, and viable cells generally could not be recovered after 24 h. it was shown that this phenomenon was due to the production of relatively high levels of acetic acid by these strains, even during growth under highly aerobic conditions, a ...19892930167
segmental colitis associated with aeromonas hydrophila.we describe a 40-yr-old woman who presented with abdominal pain and diarrhea, and who was found to have segmental colitis involving the cecum and ascending colon. aeromonas hydrophila was isolated from the stool, and her symptoms responded to appropriate antibiotic therapy. follow-up colonoscopy confirmed complete endoscopic and histologic resolution of her colitis. this report further supports the belief that aeromonas species are pathogens in healthy humans. this organism needs to be considere ...19892929569
[aeromonas hydrophila. infection in xenopus laevis].aeromonas hydrophila caused severe disease in a group of 50 xenopus leavis three weeks after being transferred from their laboratory conditions. the first clinical signs observed were marked pallor, petechiae, lethargy, anorexia, and edema. the duration of the disease was about 48 days, during which 4 animals acquired the disease and 10 died. the study of the bacteriology of the skin and skeletal muscles showed at necropsy subcutaneous edema, hemorrhage, and ascitis. aeromonas hydrophila was iso ...19892486216
occurrence of aeromonas hydrophila in wild birds.aeromonas hydrophila was isolated from 135 of a total of 1226 wild birds. it was the only bacterial species in eight birds, while other bacteria, mainly enterobacteriaceae, staphylococci and/or streptococci were identified in all other birds. the rate of isolation from aquatic birds (18.5%) was higher (p<0.001) than from birds of terrestrial habitats (3.4%). infection may also depend on dietary habits: 7.0% of the granivorous and herbivorous species, 8.4% of the omnivores and 12.0% of carnivorou ...198918679900
solubilization of fish proteins using immobilized microbial cells.cells of bacillus megaterium, aeromonas hydrophila, and pseudomonas marinoglutinosa were immobilized in calcium alginate. the immobilized cells secreted protease when held in fish meat suspension in water. the enzyme synthesis by the entrapped cells was supported by small amounts of soluble nutrients present in the meat. the secreted protease solubilized the fish meat, solubilization being optimum at ph range of 7.5 to 9.5 and at 50 degrees c. under these conditions immobilized b. megaterium was ...198918588026
bacterial colonization of cod (gadus morhua l.) and halibut (hippoglossus hippoglossus) eggs in marine aquaculture.aquaculture has brought about increased interest in mass production of marine fish larvae. problems such as poor egg quality and mass mortality of fish larvae have been prevalent. the intensive incubation techniques that often result in bacterial overgrowth on fish eggs could affect the commensal relationship between the indigenous microflora and opportunistic pathogens and subsequently hamper egg development, hatching, larval health, and ongrowth. little information about the adherent microflor ...198916347937
[aeromonas spp. and plesiomonas shigelloides in bivalves, mud, and water from the gulf of nicoya, costa rica].bivalves, mud, and surface water were collected at three different sites of the gulf of nicoya, costa rica, in search of aeromonas spp. and plesiomonas shigelloides. for their isolation, these bacteria were enriched in alkaline peptone water and streaked on macconkey agar and on brilliant green bile inositol agar. this was followed by the biochemical tests necessary for their identification. thirty-five strains of a. hydrophila, 58 of a. caviae, 43 of a. sobria, and 7 of p. shigelloides were iso ...19899700365
antibiotic resistance patterns of aeromonas spp. isolated from environmental and clinical sources.microbiologists from the international centre for diarrhoeal disease research, bangladesh (icddr,b) collected water, sediment, plant, and plankton samples from a lake and a river in dhaka, bangladesh to isolate aeromonas spp. they also took clinical isolates from patients at the dhaka hospital of icddr,b. the microbiologists identified at least 154 isolates of aeromonas and analyzed them for antibiotic susceptibility using the standard agar disc diffusion method. 92.8% of the environmental is ...198812342624
molecular cloning, characterization, and nucleotide sequence of an extracellular amylase gene from aeromonas hydrophila.the structural gene for excreted amylase from aeromonas hydrophila jmp636 has been cloned within a 2.1-kilobase smai fragment of dna. the amylase gene is transcribed from its own promoter in escherichia coli, producing a gene product of mr 49,000. the amylase gene product is secreted to the periplasm of e. coli; however, it is not excreted. nucleotide sequencing revealed an open reading frame of 1,392 base pairs corresponding to a protein of 464 amino acid residues. a potential signal peptide of ...19882449422
adhesion of clinical and environmental aeromonas isolates to hep-2 cells.a total of 63 aeromonas strains isolated from diarrhoeal faeces or water samples were tested for adhesion to hep-2 cells. an association between diarrhoea and high level adhesion was observed in that 12 of the 34 faecal isolates and none of the 29 environmental isolates yielded greater than 20 bacteria per hep-2 cell in the adhesion assay. the proportion of high adherers was significantly greater for a. sobria (57%) than for a. hydrophila isolates (19%). three of the eight faecal a. caviae isola ...19882897470
comparison of four typing methods for aeromonas species. 19882908357
expression of met-(-1) angiogenin in escherichia coli: conversion to the authentic less than glu-1 protein.a method for obtaining authentic human angiogenin utilizing an escherichia coli recombinant expression system is described. a synthetic gene encoding angiogenin was placed into a vector for direct expression under the control of a modified e. coli trp promoter. the protein was produced by the bacteria in an insoluble form and purified to homogeneity by cation-exchange and reversed-phase hplc following reduction/solubilization and reoxidation. the protein isolated was identified as met-(-1) angio ...19883071185
bacterial-induced diarrhoea.in diverse parts of the world, many bacterial strains have been recognised as the causative agents of diarrhoea. great achievements have been made in perceiving the varied mechanisms which explain their intestinal pathogenicity. these achievements include the elucidation of the 5 mechanisms of disease production by escherichia coli, the mechanisms of action of shigella, salmonella, campylobacter and yersinia, new information about antimicrobial-associated colitis caused by clostridium difficile ...19883069445
enzymatic characterization of three aeromonas species using api peptidase, api "osidase," and api esterase test kits.an enzymatic characterization of 16 strains of aeromonas species including a. hydrophila (7), a. sobria (5), and a. caviae (4) was carried out using api peptidase (strips numbered 1, 2, 3, 4, 5, and 6); api esterase and api "osidase" test strips. a total of 89 substrates was used in the assay and included 59 arylamides (aminopeptides), 10 esters, and 20 carbohydrates. all three species were remarkably uniform in their reactivities. nineteen (32%) of the arylamide substrates used were hydrolyzed ...19883072150
gastro-enteritis in australian children: studies on the aetiology of acute diarrhoea.a prospective, year-long study of the microbiology of childhood gastro-enteritis in perth showed that (i) aeromonas-associated diarrhoea was the commonest recognized bacterial cause for acute diarrhoea, especially in summer, (ii) campylobacter infections were the next most common bacterial cause, (iii) rotavirus and parasites were not significant causes of sporadic acute childhood diarrhoea, and (iv) enteric bacterial pathogens and intestinal parasites were often isolated from aboriginal childre ...19882456732
virulence properties and enterotoxin production of aeromonas strains isolated from fish.the biological activities in vivo and in vitro of 59 motile aeromonas spp. isolated from fish and water tanks were simultaneously analyzed in poikilothermic and homoiothermic systems. a total of 64.3% of the isolates tested were pathogenic for fish, and 62% of aeromonas hydrophila and a. sobria isolates either virulent or nonvirulent for fish were enterotoxigenic. although the majority of the strains were proteolytic and amylolytic and produced dnase, other activities, such as elastase and staph ...19882972627
travelers' diarrhea in west africa and mexico: fecal transport systems and liquid bismuth subsalicylate for self-therapy.the goals of this study were threefold: to compare the etiology of travelers' diarrhea in west africa and mexico, to evaluate two fecal transport systems for the recovery of enteropathogens, and to verify the efficacy of liquid bismuth subsalicylate (bss) in different locations and under different entrance criteria for disease severity. the study populations consisted of 133 european tourists in west africa and 112 american students in mexico who had suffered from travelers' diarrhea. in 60% and ...19882896219
cefotaxime-sensitive aeromonas hydrophila infection in a revascularized foot.three days following revascularization of a foot injured in a boating accident, aeromonas hydrophila cellulitis developed in the victim's foot and leg. the infection resolved with debridement and 10 days of cefotaxime therapy. a. hydrophila infection has not previously been reported in a revascularized extremity. clinical response of an a. hydrophila cellulitis to cefotaxime is likewise undescribed. our findings of a cefotaxime-sensitive aeromonas infection and its successful treatment suggests ...19882896484
epidemiology of aeromonas species in a hospital.the occurrence of aeromonas spp. in a hospital water supply, in patients and in the local community was investigated. in healthy persons outside the hospital the isolation rate was 3.6% and among hospital patients it was 6%. seven per cent of water samples yielded aeromonas strains. isolates were typed by sodium dodecyl sulphate polyacrylamide gel electrophoresis of sulphur-35 methionine labelled proteins of aeromonas isolates. no relationship between water and human isolates could be establishe ...19882896686
[microflora of the drinking water regenerated from atmospheric moisture condensate in hermetically sealed quarters].in an enclosed environment the species composition of microorganisms was quantified and metabolic processes in microbial cells isolated from the atmospheric condensate and reclaimed water were investigated. it was demonstrated that indicator microorganisms isolated from water and condensate samples (alcaligenes faecalis, citrobacter freundii, aeromonas hydrophila, acinetobacter-moraxella) were specific and differed from indicator microorganisms typical of regular tap water.19882898035
[the occurrence of antibiotic-resistant aeromonas and vibrio species in river water].262 samples of river water had been analysed for the occurrence of antibiotic resistant aeromonas and vibrio strains. the bacteria were cultured on selective media containing the antimicrobial chemotherapeutic agents oxytetracycline, chloramphenicol, streptomycin, ampicillin, kanamycin, gentamycin or trimethoprim. although it was not possible to identify antibiotic resistant bacteria of the genus vibrio, resistant strains of aeromonas hydrophila with a wide variety of resistance patterns could b ...19882899367
isolation of theileria parva schizonts from infected lymphoblastoid cells.this study set out to develop a rapid method for the isolation of schizonts from theileria parva-infected bovine and buffalo lymphoblastoid cells. parasitized lymphoblastoid cells were lysed by treatment with the cytolytic toxins, aerolysin and ah-1 hemolysin, produced by aeromonas hydrophila, and the schizonts were separated by percoll density-gradient centrifugation. light and electron microscopic examination showed that the isolated schizonts from lymphoblastoid cells infected with t. parva ( ...19882903622
microbiological and virological analysis of water from two water filtration plants and their distribution systems.the microbial flora of the water produced by two water filtration plants and their drinking water distribution system were evaluated: the pont-viau (pv) and the repentigny (re) water filtration plants. untreated water entering the plants contained 3.6 (pv) and 16.8 most probable number of infectious units (mpniu)/l (re) enteric viruses and total coliform bacteria counts were 300,000 (pv) and 500,000 cfu/l (re). treated water leaving the plant was essentially free of all the bacterial indicators ...19882906813
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