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a role for genes and environment in the causal relationship between infant rsv infection and childhood asthma. 201020053969
respiratory syncytial virus (rsv) bronchiolitis and excessive thrombocytosis. 201020064137
a single vaccination with an inactivated bovine respiratory syncytial virus vaccine primes the cellular immune response in calves with maternal antibody.the efficacy of a single dose of an inactivated bovine respiratory syncytial virus (brsv)--parainfluenaza type 3 (pi3)--mannheimia haemolytica (mh) combination vaccine, in calves positive for maternal antibodies, was established in a brsv infection study.201020064236
respiratory viral infections in infants: causes, clinical symptoms, virology, and immunology.in global terms, respiratory viral infection is a major cause of morbidity and mortality. infancy, in particular, is a time of increased disease susceptibility and severity. early-life viral infection causes acute illness and can be associated with the development of wheezing and asthma in later life. the most commonly detected viruses are respiratory syncytial virus (rsv), rhinovirus (rv), and influenza virus. in this review we explore the complete picture from epidemiology and virology to clin ...201020065326
inhibitory effect of melatonin on lung oxidative stress induced by respiratory syncytial virus infection in mice.previous research has shown that antioxidant (butylated hydroxyanisole) treatment ameliorates respiratory syncytial virus (rsv)-induced disease and lung inflammation. melatonin has been reported to exhibit a wide varieties of biological effects, including antioxidant and anti-inflammation, and has no evident toxicity and side effect. but it is not known whether melatonin would modify rsv-induced lung disease and oxidative stress. the present study was to establish the involvement of oxidative st ...201020070490
effects of tetrabromobisphenol a, a brominated flame retardant, on the immune response to respiratory syncytial virus infection in mice.effects of the brominated flame retardants (bfrs), decabrominated diphenyl ether (dbde), hexabromocyclododecane (hbcd), and tetrabromobisphenol a (tbbpa), on host immunity of mice were evaluated using respiratory syncytial virus (rsv) infection. five-week-old female mice were fed a diet containing 1% bfrs for 28days, and subsequently infected with rsv. no toxicological sign was observed in bfr-treated mice before infection. tbbpa significantly increased the pulmonary viral titer in the infected ...201020074668
a prospective molecular surveillance study evaluating the clinical impact of community-acquired respiratory viruses in lung transplant recipients.community-acquired respiratory viral infections (rvis) are common in lung transplant patients and may be associated with acute rejection and bronchiolitis obliterans syndrome (bos). the use of sensitive molecular methods that can simultaneously detect a large panel of respiratory viruses may help better define their effects.201020075787
complete genome sequence of avian paramyxovirus-3 strain wisconsin: evidence for the existence of subgroups within the serotype.the complete consensus genome sequence was determined for avian paramyxovirus (apmv) serotype 3 strain wisconsin. the genome is 16,182 nucleotides (nt) in length, consisting of six non-overlapping genes in the order of 3'-n-p/v/w-m-f-hn-l-5', with a 55-nt leader at its 3' end and a 681-nt trailer at its 5' end. comparison of the apmv-3 strain wisconsin nt and the aggregate predicted amino acid (aa) sequences with those of apmv-3 strain netherlands revealed 67% and 78%, identity, respectively. th ...201020079781
decreased replication of human respiratory syncytial virus treated with the proteasome inhibitor mg-132.many enveloped viruses require components of the host protein ubiquitin system including members of the paramyxoviridae family of viruses (piv5, sev). until recently, little has been known about the requirements of the subfamily pneumovirinae. we report here that treatment of vero cells with the proteasome inhibitor mg-132 results in the reduction of human respiratory syncytial virus (hrsv) titers by as much as 2.2log(10). inhibition of hrsv by mg-132 was only observed early in infection (4-14h ...201020080137
pulmonary surfactant phosphatidylglycerol inhibits respiratory syncytial virus-induced inflammation and infection.respiratory syncytial virus (rsv) is the most common cause of hospitalization for respiratory tract infection in young children. it is also a significant cause of morbidity and mortality in elderly individuals and in persons with asthma and chronic obstructive pulmonary disease. currently, no reliable vaccine or simple rsv antiviral therapy is available. recently, we determined that the minor pulmonary surfactant phospholipid, palmitoyl-oleoyl-phosphatidylglycerol (popg), could markedly attenuat ...201020080799
anti-inflammatory effect of muc1 during respiratory syncytial virus infection of lung epithelial cells in vitro.muc1 is a transmembrane glycoprotein expressed on the apical surface of airway epithelial cells and plays an anti-inflammatory role during airway bacterial infection. in this study, we determined whether the anti-inflammatory effect of muc1 is also operative during the respiratory syncytial virus (rsv) infection. the lung epithelial cell line a549 was treated with rsv, and the production of tnfalpha and the levels of muc1 protein were monitored temporally during the course of infection by elisa ...201020081068
multiple, non-conserved, internal viral ligands naturally presented by hla-b27 in human respiratory syncytial virus-infected cells.cytotoxic t lymphocyte (ctl)-mediated death of virus-infected cells requires prior recognition of short viral peptide antigens that are presented by human leukocyte antigen (hla) class i molecules on the surface of infected cells. the ctl response is critical for the clearance of human respiratory syncytial virus (hrsv) infection. using mass spectrometry analysis of complex hla-bound peptide pools isolated from large amounts of hrsv-infected cells, we identified nine naturally processed hla-b27 ...201020081153
epidemic viral pneumonia.two recent viral epidemics producing pneumonitis (severe acute respiratory syndrome and pandemic influenza a h1n1) have highlighted the potential for viral infections to cause respiratory failure with a significant risk of mortality. this review describes these epidemics and other causes of epidemic viral pneumonia.201020087179
the role of tnf-alpha in eosinophilic inflammation associated with rsv bronchiolitis.the purpose of our study was to investigate whether tumor necrosis factor (tnf)-alpha correlates with eosinophilic inflammation that occurs during a lower respiratory tract infection with the respiratory syncytial virus (rsv) in children. sixty children with rsv bronchiolitis (rsv group) and 20 healthy children with no respiratory symptoms (control group) were enrolled. we measured the nasal lavage fluid (nlf) th2 cytokine (il-5), proinflammatory cytokine (tnf-alpha, il-8), eosinophil-active cyt ...201020088864
withdrawn. surfactant therapy for bronchiolitis in critically ill infants.viral bronchiolitis is a common cause of respiratory failure in infants and children, and accounts for a significant portion of intensive care unit (icu) admissions during seasonal epidemics. currently there is no evidence to support the use of anything but supportive care for this disease. surfactant is a potentially promising therapy; alterations in its composition have been described in bronchiolitis, and it may play a role in the host immunity for this disease.201020091572
protecting healthcare workers from pandemic influenza: n95 or surgical masks?the successful management of an influenza pandemic will be reliant on the expertise of healthcare workers at high risk for occupationally acquired influenza. recommended infection control measures for healthcare workers include surgical masks to protect against droplet-spread respiratory transmissible infections and n95 masks to protect against aerosol-spread infections. a literature review was undertaken for evidence of superior protective value of n95 masks or surgical masks for healthcare wor ...201020095070
duration of shedding of respiratory syncytial virus in a community study of kenyan children.our understanding of the transmission dynamics of respiratory syncytial virus (rsv) infection will be better informed with improved data on the patterns of shedding in cases not limited only to hospital admissions.201020096106
structural basis of respiratory syncytial virus neutralization by motavizumab.motavizumab is approximately tenfold more potent than its predecessor, palivizumab (synagis), the fda-approved monoclonal antibody used to prevent respiratory syncytial virus (rsv) infection. the structure of motavizumab in complex with a 24-residue peptide corresponding to its epitope on the rsv fusion (f) glycoprotein reveals the structural basis for this greater potency. modeling suggests that motavizumab recognizes a different quaternary configuration of the f glycoprotein than that observed ...201020098425
distribution of airborne influenza virus and respiratory syncytial virus in an urgent care medical clinic.considerable controversy exists with regard to whether influenza virus and respiratory syncytial virus (rsv) are spread by the inhalation of infectious airborne particles and about the importance of this route, compared with droplet or contact transmission.201020100093
burden of respiratory syncytial virus in hospitalized infants and young children in amman, jordan.acute respiratory infections (ari) play a major role in hospitalizations in the middle east, but the specific viral causes are unknown. we conducted prospective viral surveillance in children <5 y of age admitted with ari and/or fever at 2 dissimilar hospitals in amman, jordan during peak respiratory syncytial virus (rsv) season. we collected prospective clinical and demographic data and obtained nose/throat swabs for testing for rsv by real-time polymerase chain reaction (rt-pcr). we obtained c ...201020100116
ldh concentration in nasal-wash fluid as a biochemical predictor of bronchiolitis severity.because the decision to hospitalize an infant with bronchiolitis is often supported by subjective criteria and objective indicators of bronchiolitis severity are lacking, we tested the hypothesis that lactate dehydrogenase (ldh), which is released from injured cells, is a useful biochemical indicator of bronchiolitis severity.201020100751
ccl20/ccr6 blockade enhances immunity to rsv by impairing recruitment of dc.chemokines are important mediators of the immune response to pathogens, but can also promote chronic inflammatory states. chemokine receptor 6 (ccr6) is found on immature dc and effector/memory t cells, and binds a single ligand, ccl20, with high affinity. here, we investigated the role of ccl20 and ccr6 in a pulmonary viral infection caused by rsv, a ubiquitous virus that can cause severe pulmonary complications. neutralization of ccl20 during rsv infection significantly reduced lung pathology ...201020101616
inactivation of respiratory syncytial virus by zinc finger reactive compounds.infectivity of retroviruses such as hiv-1 and mulv can be abrogated by compounds targeting zinc finger motif in viral nucleocapsid protein (nc), involved in controlling the processivity of reverse transcription and virus infectivity. although a member of a different viral family (pneumoviridae), respiratory syncytial virus (rsv) contains a zinc finger protein m2-1 also involved in control of viral polymerase processivity. given the functional similarity between the two proteins, it was possible ...201020102602
detection of multiple respiratory pathogens during primary respiratory infection: nasal swab versus nasopharyngeal aspirate using real-time polymerase chain reaction.in this study, we present the multiple detection of respiratory viruses in infants during primary respiratory illness, investigate the sensitivity of nasal swabs and nasopharyngeal aspirates, and assess whether patient characteristics and viral load played a role in the sensitivity. healthy infants were included at signs of first respiratory tract infection. paired nasopharyngeal aspirates and nasal swabs were collected. real-time polymerase chain reaction (pcr) was carried out for 11 respirator ...201020111881
recombinant respiratory syncytial virus f protein expression is hindered by inefficient nuclear export and mrna processing.studies of the fusion activity of respiratory syncytial virus (rsv) f protein are significantly hindered by low recombinant expression levels. while infection produces f protein levels detectable by western blot, recombinant expression produces undetectable to low levels of f protein. identifying the obstacles that hinder recombinant f protein expression may lead to improved expression and facilitate the study of f protein function. we hypothesized that nuclear localization and/or inefficient rn ...201020111897
propranolol for infantile hemangiomas: early experience at a tertiary vascular anomalies center.propranolol has recently been introduced as a novel pharmacologic treatment for infantile hemangiomas. systematic examination of this treatment in a tertiary care setting has not been described. this study explores the impact of propranolol on both proliferative and involuting hemangiomas at a tertiary vascular anomalies center.201020112413
novel human bocavirus in children with acute respiratory tract infection.human bocavirus (hbov) and hbov2, two human bocavirus species, were found in 18 and 10 of 235 nasopharyngeal aspirates, respectively, from children hospitalized with acute respiratory tract infection. our results suggest that, like hbov, hbov2 is distributed worldwide and may be associated with respiratory and enteric diseases.201020113572
[viral etiology of acute respiratory infection in children from wenzhou between 2007 and 2008].to study the viral etiology of acute respiratory infection (ari)in children from wenzhou, zhejiang between 2007 and 2008.201020113631
acute viral bronchiolitis in children- a very common condition with few therapeutic options.acute viral bronchiolitis remains a cause of substantial morbidity and health care costs in young infants. it is the most common lower respiratory tract condition and most common reason for admission to hospital in infants. many respiratory viruses have been associated with acute viral bronchiolitis although respiratory syncytial virus (rsv) remains the most frequently identified virus. most infants have a mild self limiting illness while others have more severe illness and require hospital admi ...201020113991
transmission of respiratory syncytial virus at the paediatric intensive-care unit: a prospective study using real-time pcr.transmission of respiratory syncytial virus (rsv) from children with lower respiratory tract infection (lrti) at a paediatric intensive-care unit (picu) was examined using a highly sensitive real-time pcr. twenty-four children with rsv lrti were admitted during the study period (total days of potential transmission: 239). forty-eight rsv-negative patients were followed up for rsv acquisition every 5 days (total days of exposure: 683). no single rsv transmission was documented with this highly se ...201019523052
potential role of soluble trail in epithelial injury in children with severe rsv infection.lower respiratory tract infection by respiratory syncytial virus (rsv) is a frequent cause of acute lung injury in young children and infants. studies in adults and animals suggest that tumor necrosis factor receptor (tnfr) ligands may mediate lung injury by causing apoptosis of epithelial cells. the main goal of the present study was to determine whether the tnf-related apoptosis-inducing ligand (apo2l/trail) pathway may be implicated in epithelial injury during severe rsv infection in children ...201019635930
antiviral activity of daphnoretin isolated from wikstroemia indica.the ethanol extract of wikstroemia indica was fractionated with organic solvents of different polarities, and various fractions were screened for their antiviral activity against respiratory syncytial virus (rsv) using a cytopathic effect (cpe) reduction assay. the ethyl acetate fraction was most active against rsv with 50% inhibition concentration (ic(50)) value < 3.9 microg/ml and a selectivity index (si) > 64.1. further isolation and purification of the fraction led to a purified compound, da ...201019610034
respiratory syncytial virus impairs macrophage ifn-alpha/beta- and ifn-gamma-stimulated transcription by distinct mechanisms.macrophages are the primary lung phagocyte and are instrumental in maintenance of a sterile, noninflamed microenvironment. ifns are produced in response to bacterial and viral infection, and activate the macrophage to efficiently counteract and remove pathogenic invaders. respiratory syncytial virus (rsv) inhibits ifn-mediated signaling mechanisms in epithelial cells; however, the effects on ifn signaling in the macrophage are currently unknown. we investigated the effect of rsv infection on ifn ...201019502390
incidence of common respiratory viral infections related to climate factors in hospitalized children in hong kong.hong kong has a subtropical climate and an influenza seasonality lying approximately mid-way (march-june) between those of the northern (november-march) and southern (june-september) hemispheres. respiratory syncytial virus (rsv) shares a similar seasonality to that of influenza in hong kong and is another important respiratory infection of childhood. daily virus incidence data from public hospitals in hong kong's new territory east cluster, together with hong kong climate data were obtained for ...201019631018
bronchiolitis-associated encephalopathy in critically-ill infants: an underestimated complication?to investigate the bronchiolitis-associated encephalopathy in critically ill infants.201019701864
prevalence of respiratory syncytial virus (rsv) risk factors and cost implications of immunoprophylaxis to infants 32 to 35 weeks gestation for health plans in the united states.during the period of this study, the american academy of pediatrics (aap) 2006 guidelines recommended respiratory syncytial virus (rsv) prophylaxis for infants 32 to 35 weeks gestation age (wga) with two or more of five risk factors (rfs). new recommendations have recently been published in 2009. the cost implications of expanding this list of rfs to include other evidence-based rfs like passive smoke exposure (pse), crowded living conditions (clcs), and young chronological age (yca) are unclear ...201019706010
association of human metapneumovirus with radiologically diagnosed community-acquired alveolar pneumonia in young children.to determine the involvement of human metapneumovirus (hmpv) in childhood community-acquired alveolar pneumonia (caap) and compare the demographic, clinical, and laboratory features of hmpv-associated caap and caap associated with other respiratory viruses.201019782998
respiratory syncytial virus infection and palivizumab: are families receiving accurate information?the aim of this study was to assess the accuracy of information provided by medical practitioners to families regarding the efficacy and limitations of prophylaxis with palivizumab for respiratory syncytial virus (rsv) infection in infants. a single-question survey was distributed to medical practitioners who described themselves as having both prescribed palivizumab for infants and discussed the effects of the drug with families. responses were anonymous and returned immediately after reviewing ...201019784911
survey of severe respiratory syncytial virus infection in kyoto prefecture from 2003 to 2007.respiratory syncytial virus (rsv) infection in infants can develop into a severe condition.201019761516
a multi-drug regimen for respiratory syncytial virus and parainfluenza virus infections in adult lung and heart-lung transplant recipients.respiratory syncytial virus (rsv) and parainfluenza virus (piv) can cause significant morbidity and mortality in lung and heart-lung transplant recipients. we evaluated the utility of a multi-drug protocol for the treatment of rsv- and piv-related infections.201019761558
human hepg2 cells support respiratory syncytial virus and human metapneumovirus replication.human metapneumovirus (hmpv) and human respiratory syncytial (rsv) virus cause mild to severe infections of the respiratory tract in all age groups. so far, several cell lines derived from respiratory tissues have been identified to support replication of both viruses. unfortunately, titers attained during replication differ between the both viruses within one cell line despite equal infection conditions, on the one hand giving raise to the assumption that the individual susceptibility may vary ...201019799934
[evolution of the number of rotavirus and respiratory syncytial virus infections in children hospitalised in a french university hospital between 1998 and 2005].respiratory syncytial virus (rsv) and rotavirus infections represent up to 30% of cross infections in pediatric units. as they are a major public health problem, we studied their evolution and distribution at the dijon university hospital.201019081201
human metapneumovirus: an emerging respiratory pathogen.human metapneumovirus (hmpv) is an important emerging respiratory pathogen, previously unreported in the emergency medicine literature. it is primarily associated with the clinical syndromes of bronchiolitis and pneumonia. hmpv may predispose to bacterial pneumonia; coinfection with respiratory syncytial virus may lead to increased severity of clinical disease, and complications include asthma and chronic obstructive pulmonary disease exacerbations. given its high prevalence and potential clinic ...201018584996
modeling competing infectious pathogens from a bayesian perspective: application to influenza studies with incomplete laboratory results.in seasonal influenza epidemics, pathogens such as respiratory syncytial virus (rsv) often co-circulate with influenza and cause influenza-like illness (ili) in human hosts. however, it is often impractical to test for each potential pathogen or to collect specimens for each observed ili episode, making inference about influenza transmission difficult. in the setting of infectious diseases, missing outcomes impose a particular challenge because of the dependence among individuals. we propose a b ...201021472041
part 4. interaction between air pollution and respiratory viruses: time-series study of daily mortality and hospital admissions in hong kong.background: populations in asia are not only at risk of harm to their health through environmental degradation as a result of worsening pollution problems but also constantly threatened by recurring and emerging influenza epidemics and. pandemics. situated in the area with the world's fastest growing economy and close to hypothetical epicenters of influenza transmission, hong kong offers a special opportunity for testing environmental management and public health surveillance in the region. in t ...201021446214
respiratory syncytial virus: a prioritized or neglected target? 201021426146
[frequency of respiratory viruses and clinical characteristics in children attending a care center in mexico city].to describe the frequency of respiratory viruses and clinical characteristics in children with respiratory signs and symptoms in a tertiary care center in mexico.201021271012
serious early childhood wheezing after respiratory syncytial virus lower respiratory tract illness in preterm infants.respiratory syncytial virus (rsv) lower respiratory tract infection (lri) in early life has been associated with sustained airway hyperreactivity during childhood; however, corresponding data in premature infants are sparse.201021353110
respiratory syncytial virus testing during bronchiolitis episodes of care in an integrated health care delivery system: a retrospective cohort study.bronchiolitis has the highest incidence rate of any lower respiratory infection among infants and children <2 years of age. respiratory syncytial virus (rsv) is the most common etiology of bronchiolitis. the american academy of pediatrics does not recommend routine rsv testing for infants and children with bronchiolitis. the clinical predictors of rsv testing are unknown. objectives: the aims of this study were to identify the rates and predictors of rsv testing during bronchiolitis and to explo ...201021316538
prevalence of pasteurella multocida and other respiratory pathogens in the nasal tract of scottish calves.the prevalence of pasteurella multocida, a cause of bovine respiratory disease, was studied in a random sample of beef suckler and dairy farms throughout scotland, by means of a cross-sectional survey. a total of 637 calves from 68 farms from six geographical regions of scotland were sampled between february and june 2008. deep nasal swabs were taken, and samples that were culture-positive for p multocida were confirmed by pcr. prevalence of p multocida was 17 per cent (105 of 616 calves); 47 pe ...201021257416
mortality attributable to pandemic influenza a (h1n1) 2009 in san luis potosí, mexico.acute respiratory infections are a leading cause of morbidity and mortality worldwide. starting in 2009, pandemic influenza a(h1n1) 2009 virus has become one of the leading respiratory pathogens worldwide. however, the overall impact of this virus as a cause of mortality has not been clearly defined.201021306570
using the full spectral capacity (six channels) of a real-time pcr instrument can simplify diagnostic laboratory screening and typing protocols for pandemic h1n1 influenza.timely reporting of influenza a virus subtype affects patient management. real-time pcr is a rapid and sensitive method routinely used to characterise viral nucleic acid, but the full spectral capability of the instruments is not employed.201021306574
influenza and other respiratory viruses in three central american countries.despite the disease burden imposed by respiratory diseases on children in central america, there is a paucity of data describing the etiologic agents of the disease.201021306576
synthesis of new c5-(1-substituted-1,2,3-triazol-4 or 5-yl)-2'-deoxyuridines and their antiviral evaluation.the synthesis and antiviral evaluation of a series of c5-(1,4- and 1,5-disubstituted-1,2,3-triazolo)-nucleoside derivatives is described. the key steps of this synthesis are regioselective huisgen's 1,3-dipolar cycloaddition, using either copper-catalyzed azide-alkyne cycloaddition (cuaac) or ruthenium-catalyzed azide-alkyne cycloaddition (ruaac) under microwave activation. some compounds among the 5a-l series possess activity against herpes simplex viruses 1 and 2, varicella-zoster virus, human ...201021232828
mortality and morbidity among infants at high risk receiving prophylaxis with palivizumab: a systematic literature review and meta-analysis.objectives:: a systematic literature review and meta-analysis was performed to evaluate the impact of prophylaxis with palivizumab on mortality and morbidity associated with respiratory syncytial virus infection in infants at high risk (≤35 wks of gestational age, chronic lung disease, or congenital heart disease). data sources:: medline, embase, and current contents were used. medline was searched from january 1, 1990 to may 16, 2007. the bibliographies of accepted studies and recent reviews an ...201021200358
local cytokine response upon respiratory syncytial virus infection.respiratory syncytial virus (rsv) is the leading cause of childhood hospitalization and respiratory distress and has been recognized for several decades as a major health and economic burden worldwide. this virus has developed several virulence mechanisms to impair the establishment of a protective immune response to re-infection. accordingly, inefficient immunological memory is usually generated after exposure to this pathogen. furthermore, it has been shown that rsv can actively promote the in ...201021195729
clinical prediction rule for rsv bronchiolitis in healthy newborns: prognostic birth cohort study.our goal was to determine predictors of respiratory syncytial virus (rsv) lower respiratory tract infection (lrti) among healthy newborns.201021187309
respiratory syncytial virus represses glucocorticoid receptor-mediated gene activation.respiratory syncytial virus (rsv) is a common cause of bronchiolitis in infants. although antiinflammatory in nature, glucocorticoids have been shown to be ineffective in the treatment of rsv-induced bronchiolitis and wheezing. in addition, the effectiveness of glucocorticoids at inhibiting rsv-induced proinflammatory cytokine production in cell culture has been questioned. in this study, we have investigated the effect of rsv infection on glucocorticoid-induced gene activation in lung epitheliu ...201021190962
decreased serum antibody responses to recombinant pneumocystis antigens in hiv-infected and uninfected current smokers.serologic studies can provide important insights into the epidemiology and transmission of pneumocystis jirovecii. exposure to p. jirovecii can be assessed by serum antibody responses to recombinant antigens from the major surface glycoprotein (msgc), although factors that influence the magnitude of the antibody response are incompletely understood. we determined the magnitudes of antibody responses to p. jirovecii in comparison to adenovirus and respiratory syncytial virus (rsv) in hiv-infected ...201021191078
respiratory outcomes, utilization and costs 12 months following a respiratory syncytial virus diagnosis among commercially insured late-preterm infants.to determine, among a commercially-insured population of late-preterm infants, utilization of healthcare resources and costs during the 1 year following a diagnosis of respiratory syncytial virus lower respiratory infection (rsv lri).201021192761
pulmonary surfactant in respiratory syncytial virus bronchiolitis: the role in pathogenesis and clinical implications.respiratory syncytial virus (rsv) bronchiolitis is the leading cause of lower respiratory tract infection, and the most frequent reason for hospitalization among infants throughout the world. in addition to the acute consequences of the disease, rsv bronchiolitis in early childhood is related to further development of recurrent wheezing and asthma. despite the medical and economic burden of the disease, therapeutic options are limited to supportive measures, and mechanical ventilation in severe ...201021194166
aik-c measles vaccine expressing fusion protein of respiratory syncytial virus induces protective antibodies in cotton rats.respiratory syncytial virus (rsv) is the most common cause of respiratory infection in infants, and no vaccine is available. in this report, recombinant aik-c measles vaccines, expressing the rsv g or f protein of subgroup a (mvaik/rsv/g or f), were investigated as a rsv vaccine candidate. mvaik/rsv/g or f had the original ts phenotype and expressed rsv/g or f protein. cross-reactive neutralizing antibodies against rsv subgroups a and b were detected in cotton rats immunized intramuscularly with ...201021185852
the impact of prophylaxis on paediatric intensive care unit admissions for rsv infection: a retrospective, single-centre study.respiratory syncytial virus (rsv) is the leading cause of lower respiratory tract infections and hospitalizations in children aged <2 years. the aim of this retrospective, single-centre study was to examine the characteristics of patients admitted to a paediatric intensive care unit (picu) with rsv infection following the implementation of a rsv prophylaxis programme. electronic hospital medical records of all picu admissions for rsv infection were searched from 2003 to 2009. data on baseline de ...201021174120
[a pathogenic and clinical study of 882 cases of adult influenza-like illness in guangzhou].this study was undertaken to describe the viral etiology and clinical features in patients with influenza-like illness (ili) in guangzhou.201021176504
high frequency of repeated infections due to emerging genotypes of human respiratory syncytial viruses among children during eight successive epidemic seasons in japan.in eight successive seasons (2001 to 2009), a total of 726 human respiratory syncytial virus (hrsv) infections from a total of 1,560 children with acute lower respiratory tract illness were identified. molecular analysis of the attachment (g) protein gene confirmed that 52 (7.8%) children were infected more than once with any of the 3 genotypes of hrsv-a (genotypes ga5, na1, and na2) and/or 6 genotypes of hrsv-b (genotypes ba4, ba5, and ba7 to ba10). repeated infections in 46 cases (82.1%) occur ...201021177891
evaluation of the 3m rapid detection test for respiratory syncytial virus (rsv) in children during the early stages of the 2009 rsv season.we report the results of the 3m rapid detection respiratory syncytial virus (rsv) assay. this study includes pediatric patient results from nasopharyngeal swabs submitted from october to december 2009. there was a sensitivity of 74% and specificity approaching 100% compared to the pcr-based xtag respiratory viral panel.201021177903
[effect of qingkailing injection on children with respiratory syncytial virus pneumonia of phlegm-heat obstructing fei syndrome pattern at different time-points].to evaluate the curative effect of qingkailing injection (qkl) for treatment of children's respiratory syncytial virus pneumonia of phlegm-heat obstructing fei syndrome pattern (svp-ph) depending upon main symptom assessment.201021180082
the burden of hospitalized lower respiratory tract infection due to respiratory syncytial virus in rural thailand.we describe the epidemiology of hospitalized rsv infections for all age groups from population-based surveillance in two rural provinces in thailand.201021152047
health-related quality of life in preschool children in five health conditions.to test the responsiveness of the infant/toddler quality of life questionnaire (itqol) to five health conditions. in addition, to evaluate the impact of the child's age and gender on the itqol domain scores.201021153564
serum sp-d levels as a biomarker of lung injury in respiratory syncytial virus bronchiolitis.to evaluate whether sp-d concentration is a useful biomarker of the severity of respiratory syncytial virus (rsv) bronchiolitis, we determined sp-d concentrations in patients with rsv bronchiolitis with or without chronic heart disease. we enrolled 52 patients who had been diagnosed with rsv bronchiolitis and required admission to the hospital at the department of pediatrics of fukushima medical university school of medicine from 2004 through 2005. these patients were divided into two groups: gr ...201021154623
performance of a rapid antigen test (binax now® rsv) for diagnosis of respiratory syncytial virus compared with real-time polymerase chain reaction in a pediatric population.infants from alaska's yukon-kuskokwim delta (ykd) have a high respiratory syncytial virus (rsv) hospitalization rate (104/1000/yr). appropriate patient management requires rapid and accurate rsv diagnosis. antigen-based methods are often used in clinical settings, but these tests can lack sensitivity.201021163694
serum sp-d levels as a biomarker of lung injury in respiratory syncytial virus bronchiolitis.to evaluate whether sp-d concentration is a useful biomarker of the severity of respiratory syncytial virus (rsv) bronchiolitis, we determined sp-d concentrations in patients with rsv bronchiolitis with or without chronic heart disease. we enrolled 52 patients who had been diagnosed with rsv bronchiolitis and required admission to the hospital at the department of pediatrics of fukushima medical university school of medicine from 2004 through 2005. these patients were divided into two groups: gr ...201021171185
[etiology of community acquired pneumonia in children 2-59 months old in two ecologically different communities from peru].community acquired pneumonia (cap) is a major public health problem, being the most affected the pediatric population. the aim of this study was to compare the etiology, clinical and radiological features of cap in under- five-year-old children.201021132248
the use of a neonatal mouse model to study respiratory syncytial virus infections.respiratory syncytial virus (rsv) infection is the most significant cause of viral death in infants worldwide. the significant morbidity and mortality associated with this disease underscores the urgent need for the development of an rsv vaccine. the development of an rsv vaccine has been hampered by our limited understanding of the human host immune system, which plays a significant role in rsv pathogenesis, susceptibility and vaccine efficacy. as a result, animal models have been developed to ...201021133663
management of rsv infections in adult recipients of hematopoietic stem cell transplantation.respiratory syncytial virus (rsv) is a common cause of seasonal respiratory viral infection in patients who have undergone hematopoietic stem cell transplantation. rsv usually presents as an upper respiratory tract infection in this patient population but may progress rapidly to lower respiratory tract infection. available therapies that have been used for the treatment of rsv infections are limited to ribavirin, intravenous immunoglobulin, and palivizumab. the use of aerosolized ribavirin, alon ...201021139081
influence of lipids on the interfacial disposition of respiratory syncytical virus matrix protein.the propensity of a matrix protein from an enveloped virus of the mononegavirales family to associate with lipids representative of the viral envelope has been determined using label-free methods, including tensiometry and brewster angle microscopy on lipid films at the air-water interface and atomic force microscopy on monolayers transferred to ots-treated silicon wafers. this has enabled factors that influence the disposition of the protein with respect to the lipid interface to be characteriz ...201021141948
antiviral and lung protective activity of a novel rsv fusion inhibitor in a mouse model.respiratory syncytial virus (rsv) causes bronchiolitis in young children and common colds in adults. there is no licensed vaccine, and prophylactic treatment with palivizumab is very expensive and limited to high risk infants. ribavirin is used as an antiviral treatment in infants and immunosuppressed patients, and its use is limited due to side effects, toxicity to the recipient and staff and evidence of marginal clinical efficacy. we therefore studied the in vivo kinetics, antiviral and protec ...201021148224
t cell receptor clonotype influences epitope hierarchy in the cd8+ t cell response to respiratory syncytial virus infection.cd8+ t cell responses are important for recognizing and resolving viral infections. to better understand the selection and hierarchy of virus-specific t cell responses, we compared the t cell receptor (tcr) clonotype in parent and hybrid strains of respiratory syncytial virus-infected mice. k(d)m2(82-90) (syigsinni) in balb/c and d(b)m(187-195) (naitnakii) in c57bl/6 are both dominant epitopes in parent strains but assume a distinct hierarchy, with k(d)m2(82-90) dominant to d(b)m(187-195) in hyb ...201021118816
effects of human respiratory syncytial virus, metapneumovirus, parainfluenza virus 3 and influenza virus on cd4+ t cell activation by dendritic cells.background: human respiratory syncytial virus (hrsv), and to a lesser extent human metapneumovirus (hmpv) and human parainfluenza virus type 3 (hpiv3), re-infect symptomatically throughout life without antigenic change, suggestive of incomplete immunity. one causative factor is thought to be viral interference with dendritic cell (dc)-mediated stimulation of cd4+ t cells. methodology, principal findings: we infected human monocyte-derived dc with purified hrsv, hmpv, hpiv3, or influenza a virus ...201021124776
time series model to predict burden of viral respiratory illness on a pediatric intensive care unit.in the united states, viral respiratory infections are a leading cause of illness and hospitalization in young children. caring for children with severe viral respiratory illness can have a substantial impact on resource utilization in the pediatric intensive care unit (picu). the objective was to build a robust model that captures the periodicity of severe pediatric viral respiratory illness and forecasts the incidence of viral respiratory illness in the picu.201021127319
quantitative proteomic analysis of a549 cells infected with human respiratory syncytial virus subgroup b using silac coupled to lc-ms/ms.human respiratory syncytial virus (hrsv) is a leading cause of serious lower respiratory tract infections in infants. the virus has two subgroups a and b, which differ in prevalence and (nucleotide) sequence. the interaction of subgroup a viruses with the host cell is relatively well characterized, whereas for subgroup b viruses it is not. therefore quantitative proteomics was used to investigate the interaction of subgroup b viruses with a549 cells, a respiratory cell line. changes in the cellu ...201021110324
[experimental research on anti-respiratory syncytial virus effect in vitro of earthworm coelomic fluid].to explore the antivirus function in vitro of earthworm coelomic fluid (ecf) by researching its effect on inhibiting respiratory syncytial virus (rsv).201021110430
[research on the methods for titrating respiratory syncytial virus].respiratory syncytial virus (rsv) is the most common cause of lower respiratory infection in infants. it is very important to quantitative assay of rsv titer in the study on rsv pathogenesis, candidate vaccine and antiviral treatment. therefore, we develop real-time quantitative pcr (q-pcr) assay and enzyme immunospots (eis) for titrating rsv and compare them with traditional 50% tissue culture infectious doses (tcid50).201021110441
detection of respiratory syncytial virus using nanoparticle amplified immuno-polymerase chain reaction.in traditional immuno-polymerase chain reaction (immuno-pcr), a single antibody recognition event is associated with one to three dna tags, which are subsequently amplified by pcr. here we describe a nanoparticle-amplified immuno-pcr (npa-ipcr) assay that combines antibody recognition of enzyme-linked immunosorbent assay (elisa) with a 50-fold nanoparticle valence amplification step prior to tag amplification by pcr. the assay detects a respiratory syncytial virus (rsv) surface protein using an ...201021111702
childhood pneumonia: a neglected, climate-sensitive disease? 201021111894
[etiology and epidemic characteristics of hospital acquired pneumonia in children].to investigate the etiology and epidemic characteristics of hospital acquired pneumonia (hap) in children.201021055271
[activation of tlr3 pathway in the pathogenesis of nephrotic syndrome induced by respiratory syncytial virus in rat model].to explore the activation of toll-like receptor 3 (tlr3) pathway on the process of respiratory syncytial virus (rsv) induced nephropathy.201020848777
human parainfluenza virus type 3 (hpiv 3) community-acquired pneumonia (cap) mimicking pertussis in an adult: the diagnostic importance of hoarseness and monocytosis.background: there are relatively few causes of acute community-acquired pneumonias (caps) in adults associated with prolonged cough. in adults the most common acute caps with a prominent and persistent nonproductive cough are due to mycoplasma pneumoniae, chlamydophilia (chlamydia) pneumoniae, or bordetella pertussis (pertussis). pertussis is an underrecognized and underappreciated cause of cap in adults. different from classic pertussis in children, pertussis in adults presents with prolonged d ...201020888644
allergic sensitization is associated with rhinovirus-, but not other virus-, induced wheezing in children.data on the link between atopy and viral wheeze are limited. aim: to evaluate the association between ige sensitization and viral infection in wheezing children.201020977499
respiratory syncytial virus and asthma: still no final answer. 201020978023
circulating antibody-secreting cells during acute respiratory syncytial virus infection in adults.the specificity and duration of circulating human antibody-secreting cells (ascs) after vaccination have been well described, but characteristics of ascs during acute respiratory infections have not been well studied.201020979459
assembly and immunological properties of newcastle disease virus-like particles containing the respiratory syncytial virus f and g proteins.human respiratory syncytial virus (rsv) is a serious respiratory pathogen in infants and young children as well as elderly and immunocompromised populations. however, no rsv vaccines are available. we have explored the potential of virus-like particles (vlps) as an rsv vaccine candidate. vlps composed entirely of rsv proteins were produced at levels inadequate for their preparation as immunogens. however, vlps composed of the newcastle disease virus (ndv) nucleocapsid and membrane proteins and c ...201020980510
bayesian inference of the number of factors in gene-expression analysis: application to human virus challenge studies.nonparametric bayesian techniques have been developed recently to extend the sophistication of factor models, allowing one to infer the number of appropriate factors from the observed data. we consider such techniques for sparse factor analysis, with application to gene-expression data from three virus challenge studies. particular attention is placed on employing the beta process (bp), the indian buffet process (ibp), and related sparseness-promoting techniques to infer a proper number of facto ...201021062443
viral respiratory tract infections among patients with acute undifferentiated fever in vietnam.to investigate the proportion of viral respiratory tract infections among acute undifferentiated fevers (aufs) at primary health facilities in southern vietnam during 2001-2005, patients with auf not caused by malaria were enrolled at twelve primary health facilities and a clinic for malaria control program. serum was collected on first presentation (t0) and after 3 weeks (t3) for serology. after exclusion of acute dengue infection, acute and convalescent serum samples from 606 patients were usi ...201021073032
performance of a nurse-led paediatric point of care service for respiratory syncytial virus testing in secondary care.to evaluate respiratory syncytial virus (rsv)-point-of-care-testing (poct) performance among paediatric patients with respiratory symptoms, using the binaxnow(®) rsv assay performed by trained nurses on the paediatric ward, and compare results with those obtained by real-time polymerase chain reaction (pcr).201021073893
[two cases of symptomatic west syndrome suffering from severe respiratory syncytial virus-induced bronchiolitis].we report two cases of symptomatic west syndrome with severe respiratory syncytial virus (rsv)-induced bronchiolitis: one was a 9-month-old boy who was hospitalized for shock, and the other was a 15-month-old boy in pre-shock condition. both cases needed mechanical ventilation for approximately 2 weeks. seizures from the primary disease worsened in both patients during the infection, and both needed long periods of hospitalization, which resulted in a considerable reduction in their quality of l ...201021077358
inflammatory responses to acute pneumovirus infection in neonatal mice.the innate immune responses of neonates differ dramatically from those of adults. here we examine the acute inflammatory responses of neonatal and weanling mice infected with pneumonia virus of mice (pvm), a rodent pathogen (family paramyxoviridae, genus pneumovirus) that replicates the sequelae of severe respiratory syncytial virus infection.201021078159
evaluation of therapeutics for rsv: an important step forward. 201021079265
treatment of respiratory syncytial virus with palivizumab: a systematic review.palivizumab has proven efficacy for prophylaxis of respiratory syncytial virus (rsv) in infants with prematurity or congenital heart disease. despite a paucity of data, palivizumab is sometimes used to prevent progression when high-risk patients present with upper respiratory tract infection (urti) due to rsv, or as therapy when any patients present with severe lower respiratory tract infection (lrti) caused by rsv.201021080142
efficient lung recruitment of respiratory syncytial virus-specific th1 cells induced by recombinant bacillus calmette-guérin promotes virus clearance and protects from infection.infection by the respiratory syncytial virus (rsv) can cause extensive inflammation and lung damage in susceptible hosts due to a th2-biased immune response. such a deleterious inflammatory response can be enhanced by immunization with formalin- or uv-inactivated rsv, as well as with vaccinia virus expressing the rsv-g protein. recently, we have shown that vaccination with rbcg-expressing rsv ags can prevent the disease in the mouse. to further understand the immunological mechanisms responsible ...201021084664
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