Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| androgen dependent transcription of a mouse prostatic protein gene, psp94: involvement of estrogen receptors. | prostatic secretory protein 94 (psp94) is a prostatic protein found in both humans and rodents. as with other prostatic proteins, expression of this protein is regulated by androgens. in order to understand the androgen-responsive transcriptional regulation mechanisms involved, the present study aimed to identify and characterize the promoter activity of the gene. the 5'flanking (5'f) region of mouse psp94 (mpsp94) gene was cloned and introduced into a vector upstream of the luciferase reporter ... | 2011 | 21856419 |
| theoretical analysis of the dose dependence of the oxygen enhancement ratio and its relevance for clinical applications. | abstract: background: the increased resistance of hypoxic cells to ionizing radiation is usually believed to be the primary reason for treatment failure in tumors with oxygen-deficient areas. this oxygen effect can be expressed quantitatively by the oxygen enhancement ratio (oer). here we investigate theoretically the dependence of the oer on the applied local dose for different types of ionizing irradiation and discuss its importance for clinical applications in radiotherapy for two scenarios: ... | 2011 | 22172079 |
| a method for detecting genetic toxicity using the rna synthesis response to dna damage. | to date, biological risk assessment studies of chemicals that induce dna lesions have been primarily based on the action of dna polymerases during replication. however, dna lesions interfere not only with replication but also with transcription. therefore, detecting the damaging effects of dna lesions during transcription might be important for estimating the safety of chemical mutagens and carcinogens. however, methods to address these effects have not been developed. here, we report a simple, ... | 2011 | 22008527 |
| synergizing metabolic flux analysis and nucleotide sugar metabolism to understand the control of glycosylation of recombinant protein in cho cells. | the glycosylation of recombinant proteins can be altered by a range of parameters including cellular metabolism, metabolic flux and the efficiency of the glycosylation process. we present an experimental set-up that allows determination of these key processes associated with the control of n-linked glycosylation of recombinant proteins. | 2011 | 22008152 |
| anti-inflammatory activities of selected synthetic homoisoflavanones. | four homoisoflavanones of the 3-benzylidene-4-chromanone type, some of which were previously isolated from caesalpinia pulcherrima, were synthesised to determine their anti-inflammatory activity and cytotoxicity. a range of four different homoisoflavanones (compounds 4a-4d) were synthesised from the corresponding substituted phenols.(1)h- and (13)c-nmr data together with high-resolution mass spectroscopy data were employed to elucidate the structures. anti-inflammatory activity was determined in ... | 2011 | 21950651 |
| molecular and cellular pharmacology of the hypoxia-activated prodrug th-302. | th-302 is a 2-nitroimidazole triggered hypoxia-activated prodrug (hap) of bromo isophosphoramide mustard currently undergoing clinical evaluation. here we describe th-302 broad spectrum activity, hypoxia-selective activation, and mechanism of action. the concentration x time (ct) dependence of th-302 activation was examined as a function of oxygen concentration, with reference to the prototypic hap tirapazamine, and demonstrated superior oxygen-inhibition of cytotoxicity and much improved dose-p ... | 2011 | 22147748 |
| cell-line specific protection by berry polyphenols against hydrogen peroxide challenge and lack of effect on metabolism of amyloid precursor protein. | amyloid precursor protein (app) altered metabolism, aβ-overproduction/aggregation and oxidative stress are implicated in the development of alzheimer's disease pathology. based on our previous data indicating that administration of a polyphenol-rich (prb) blueberry extract (from wild vaccinium angustifolium) is memory enhancing in healthy mice and in order to delineate the neuroprotective mechanisms, this study investigated the antioxidant effects of prb in h(2) o(2) -induced oxidative damage, a ... | 2011 | 22086824 |
| standardization of a fluorescent-based quantitative adhesion assay to study attachment of taenia solium oncosphere to epithelial cells in vitro. | to fully understand the preliminary stages of taenia solium oncosphere attachment in the gut, adequate tools and assays are necessary to observe and quantify this event that leads to infection. a fluorescent-based quantitative adhesion assay, using biotinylated activated-oncospheres and monolayers of chinese hamster ovary cells (cho-k1) or human intestinal monolayer cells (int-407, hct-8 or ht-29), was developed to study initial events during the infection of target cells and to rapidly quantify ... | 2011 | 22178422 |
| acid stimulation (sour taste) elicits gaba and serotonin release from mouse taste cells. | several transmitter candidates including serotonin (5-ht), atp, and norepinephrine (ne) have been identified in taste buds. recently, γ-aminobutyric acid (gaba) as well as the associated synthetic enzymes and receptors have also been identified in taste cells. gaba reduces taste-evoked atp secretion from receptor cells and is considered to be an inhibitory transmitter in taste buds. however, to date, the identity of gabaergic taste cells and the specific stimulus for gaba release are not well un ... | 2011 | 22028776 |
| clmp is required for intestinal development, and loss-of-function mutations cause congenital short-bowel syndrome. | background & aims: short-bowel syndrome usually results from surgical resection of the small intestine for diseases such as intestinal atresias, volvulus, and necrotizing enterocolitis. patients with congenital short-bowel syndrome (csbs) are born with a substantial shortening of the small intestine, to a mean length of 50 cm, compared with a normal length at birth of 190-280 cm. they also are born with intestinal malrotation. because csbs occurs in many consanguineous families, it is considered ... | 2011 | 22155368 |
| qualitative and quantitative cellular glycomics of glycosphingolipids based on rhodococcal endoglycosylceramidase-assisted glycan cleavage, glycoblotting-assisted sample preparation, and matrix-assisted laser desorption ionization tandem time-of-flight mass spectrometry analysis. | glycosphingolipids (gsls) are crucially important components of the cellular membrane, where they comprise microdomains with many critical biological functions. despite this fact, qualitative and quantitative techniques for the analysis of gsls still lag behind the needs of researchers. in this study, a reliable procedure for the elucidation of cellular gsl-glycomes was established based on (a) enzymatic glycan cleavage by endoglycosylceramidases derived from rhodococcus sp. in combination with ... | 2011 | 21965662 |
| polynucleotide kinase and aprataxin-like forkhead-associated protein (palf) acts as both a single-stranded dna endonuclease and a single-stranded dna 3' exonuclease and can participate in dna end joining in a biochemical system. | polynucleotide kinase and aprataxin-like forkhead-associated protein (palf, also called aprataxin- and pnk-like factor (aplf)) has been shown to have nuclease activity and to use its forkhead-associated domain to bind to x-ray repair complementing defective repair in chinese hamster cells 4 (xrcc4). because xrcc4 is a key component of the ligase iv complex that is central to the nonhomologous dna end joining (nhej) pathway, this raises the possibility that palf might play a role in nhej. for thi ... | 2011 | 21885877 |
| a novel model and molecular therapy for z alpha-1 antitrypsin deficiency. | animal models that closely resemble human disease can present a challenge. particularly so in alpha-1 antitrypsin deficiency (α(1)atd), as the mouse alpha-1 antitrypsin (α(1)at) cluster encodes five highly related genes compared with the one in humans. the mouse pi2 homologue is closest to the α(1)at human gene. we have changed the equivalent mouse site that results in the z variant in man (glu342lys) and made both the "m" and "z" mouse pi2 α(1)at proteins. we have tested the ability of a small- ... | 2011 | 22076419 |
| Upregulated expression of indoleamine 2, 3-dioxygenase in CHO cells induces apoptosis of competent T cells and increases proportion of Treg cells. | The inflammatory enzyme indoleamine 2, 3-dioxygenase (IDO) participates in immune tolerance and promotes immune escape of IDO+ tumors. A recent hypothesis suggested that IDO may contribute to the differentiation of new T regulatory cells (Tregs) from naive CD4+ T cells. In this study we investigated the role of IDO in induction of immunosuppression in breast cancer by increasing the apoptosis of T cells and the proportion of Tregs. | 2011 | 21917155 |
| Functional analysis of arylamine N-acetyltransferase 1 (NAT1) NAT1*10 haplotypes in a complete NATb mRNA construct. | N-acetyltransferase 1 (NAT1) catalyzes N-acetylation of arylamines as well as the O-acetylation of N-hydroxylated arylamines. O-acetylation leads to the formation of electrophilic intermediates that result in DNA adducts and mutations. NAT1*10 is the most common variant haplotype and is associated with increased risk for numerous cancers. NAT1 is transcribed from a major promoter, NATb, and an alternative promoter, NATa, resulting in messenger RNAs (mRNAs) with distinct 5'-untranslated regions ( ... | 2011 | 22114069 |
| Pharmacological Effect of TRK-380, a Novel Selective Human ß(3)-Adrenoceptor Agonist, on Mammalian Detrusor Strips. | OBJECTIVE: To clarify the potential of TRK-380 as a drug for overactive bladder in humans by evaluating the agonistic activities for human ß-adrenergic receptors (ß-ARs) and the relaxing effects on isolated detrusor strips. METHODS: The agonistic activities for human ß-ARs were evaluated in SK-N-MC cells (for human ß(3)-ARs) and Chinese hamster ovary cells expressing human ß(1)- or human ß(2)-ARs using the cyclic adenosine monophosphate accumulation assay. The relaxing effects on the resting ten ... | 2011 | 22197203 |
| assessing efficacy of combined riboflavin and uv-a light (365 nm) treatment of acanthamoeba trophozoites. | to assess the acanthamoeba trophozoite viability in vitro and treatment of acanthamoeba keratitis in a hamster model using ultraviolet light a (uv-a) and riboflavin (b2). | 2011 | 22058341 |
| Down regulation effect of Rosmarinus officinalis polyphenols on cellular stress proteins in rat pheochromocytoma PC12 cells. | Polyphenols are known to exhibit wide spectrum of benefit for brain health and to protect from several neurodegenerative diseases. The present study was sought to determine the neuroprotective effects of Rosmarinus officinalis' polyphenols (luteolin, carnosic acid, and rosmarinic acid) through the investigation of stress-related proteins. We carried out measurement of the expression of heat-shock protein (Hsp) 47 promoter in heat stressed Chinese hamster ovary transfected cells. We performed pro ... | 2011 | 21861121 |
| The antipsychotic drug loxapine is an opener of the Na+-activated potassium channel Slack (slo2.2). | Sodium-activated (KNa) channels have been suggested to set the resting potential, modulate slow after-hyper polarizations, and control bursting behaviour or spike frequency adaptation (Bhattacharjee and Kaczmarek, 2005). One of the genes that encodes KNa channels is called Slack (Kcnt1, Slo2.2). Recent studies have found that Slack channels are highly expressed in nociceptive dorsal root ganglionic (DRG) neurons and were shown to modulate their firing frequency (Nuwer, et al., 2010). Therefore, ... | 2011 | 22171093 |
| Temperature dependent ultrasonic characterization of biological media. | Quantitative ultrasound (QUS) is an imaging technique that can be used to quantify tissue microstructure giving rise to scattered ultrasound. Other ultrasonic properties, e.g., sound speed and attenuation, of tissues have been estimated versus temperature elevation and found to have a dependence with temperature. Therefore, it is hypothesized that QUS parameters may be sensitive to changes in tissue microstructure due to temperature elevation. Ultrasonic backscatter experiments were performed on ... | 2011 | 21973375 |
| Genotoxicity assessment of ethylenediamine dinitrate (EDDN) and diethylenetriamine trinitrate (DETN). | Ethylenediamine dinitrate (EDDN) and diethylenetriamine trinitrate (DETN) are relatively insensitive explosive compounds that are being explored as safe alternatives to other more sensitive compounds. When used in combination with other high explosives they are an improvement and may provide additional safety during storage and use. The genetic toxicity of these compounds was evaluated to predict the potential adverse human health effects from exposure by using a standard genetic toxicity test b ... | 2011 | 21944905 |
| membrane-lipid therapy in operation: the hsp co-inducer bgp-15 activates stress signal transduction pathways by remodeling plasma membrane rafts. | aging and pathophysiological conditions are linked to membrane changes which modulate membrane-controlled molecular switches, causing dysregulated heat shock protein (hsp) expression. hsp co-inducer hydroxylamines such as bgp-15 provide advanced therapeutic candidates for many diseases since they preferentially affect stressed cells and are unlikely have major side effects. in the present study in vitro molecular dynamic simulation, experiments with lipid monolayers and in vivo ultrasensitive fl ... | 2011 | 22174906 |
| Fluorescence excitation-emission matrix (EEM) spectroscopy for rapid identification and quality evaluation of cell culture media components. | The application of fluorescence excitation-emission matrix (EEM) spectroscopy to the quantitative analysis of complex, aqueous solutions of cell culture media components was investigated. These components, yeastolate, phytone, recombinant human insulin, eRDF basal medium, and four different chemically defined (CD) media, are used for the formulation of basal and feed media employed in the production of recombinant proteins using a Chinese Hamster Ovary (CHO) cell based process. The comprehensiv ... | 2011 | 22054082 |
| engagement of αiibβ3 (gpiib/iiia) with ανβ3 integrin mediates interaction of melanoma cells with platelets: a connection to hematogenous metastasis. | a mutual relationship exists between metastasizing tumor cells and components of the coagulation cascade. the exact mechanisms as to how platelets influence blood-borne metastasis, however, remain poorly understood. here, we used murine b16 melanoma cells to observe functional aspects of how platelets contribute to the process of hematogenous metastasis. we found that platelets interfere with a distinct step of the metastasis cascade, as they promote adhesion of melanoma cells to the endothelium ... | 2011 | 22102277 |
| Light exposure and cell viability in fluorescence microscopy. | Test systems for measuring cell viability in optical microscopy (based on colony formation ability or lysosomal integrity) were established and applied to native cells as well as to cells incubated with fluorescence markers or transfected with genes encoding for fluorescent proteins. Human glioblastoma and Chinese hamster ovary cells were irradiated by various light doses, and maximum doses where at least 90% of the cells survived were determined. These tolerable light doses were in the range be ... | 2011 | 22126439 |
| [role of fibrinogen bbeta-chain d-region 454-458 residues for assembly and secretion of intact fibrinogen]. | to examine the role of fibrinogen bbeta-chain d region in the assembly and/or secretion of multichain protein, we synthesized eight variant fibrinogens with truncated bbeta-chains in the c terminal region, terminating with 454, 455, 456 or 458 residues, and with substitution at bbeta-455arg by lys, ile, ala or asp in chinese hamster ovary (cho) cells. | 2011 | 21942083 |
| Development and characterization of mouse monoclonal antibodies reactive with chicken CD83. | This study was carried out to develop and characterize mouse monoclonal antibodies (mAbs) against chicken CD83 (chCD83), a membrane-bound glycoprotein belonging to the immunoglobulin superfamily that is primarily expressed on mature dendritic cells (DCs). A recombinant chCD83/IgG4 fusion protein containing the extracellular region of chCD83 was expressed in Chinese Hamster Ovary (CHO) cells and isolated from the spent cell culture medium by protein G affinity chromatography. The extracellular re ... | 2011 | 22197010 |
| [Analysis of the transcriptional profiling of cell cycle regulatory networks of recombinant Chinese hamster ovary cells in batch and fed-batch cultures]. | In the light of Chinese hamster ovary (CHO) cell line 11G-S expressing human recombinant pro-urokinase, the differences of gene expression levels of the cells in different growth phases in both batch and fed-batch cultures were revealed by using gene chip technology. Then, based on the known cell cycle regulatory networks, the transcriptional profiling of the cell cycle regulatory networks of the cells in batch and fed-batch cultures was analyzed by using Genmapp software. Among the approximate ... | 2011 | 22097809 |
| Hybrid- and Complex-type N-glycans are not Essential for Newcastle Disease Virus Infection and Fusion of Host Cells. | N-linked glycans are composed of three major types: high-mannose, hybrid, or complex. The functional role of hybrid- and complex-type N-glycans in Newcastle disease virus (NDV) infection and fusion was examined in N-acetylglucosaminyltransferase I (GnT I)-deficient Lec1 cells, a mutant Chinese hamster ovary (CHO) cell incapable of synthesizing hybrid- and complex-type N-glycans. We used recombinant NDV expressing green fluorescence protein (GFP) or red fluorescence protein (RFP) to monitor NDV i ... | 2011 | 21964725 |
| Role of interaction of XPF with RPA in nucleotide excision repair. | Nucleotide excision repair (NER) is a very important defense system against various types of DNA damage, and it is necessary for maintaining genomic stability. The molecular mechanism of NER has been studied in considerable detail, and it has been shown that proper protein-protein interactions among NER factors are critical for efficient repair. A structure-specific endonuclease, XPF-ERCC1, which makes the 5' incision in NER, was shown to interact with a single-stranded DNA binding protein, RPA. ... | 2011 | 21875596 |
| dominant-negative control of camp-dependent iks upregulation in human long-qt syndrome type 1. | rationale: the mutation a341v in the s6 transmembrane segment of kcnq1, the α-subunit of the slowly activating delayed-rectifier k(+) (i(ks)) channel, predisposes to a severe long-qt1 syndrome with sympathetic-triggered ventricular tachyarrhythmias and sudden cardiac death. objective: several genetic risk modifiers have been identified in a341v patients, but the molecular mechanisms underlying the pronounced repolarization phenotype, particularly during β-adrenergic receptor stimulation, remain ... | 2011 | 22095730 |
| conjugation to octa-arginine via disulfide bonds confers solubility to denatured proteins in physiological solution and enables efficient cell internalization. | some protein transduction methods have already been developed for regenerative medicine application. these methods can be applied to soluble proteins but not to insoluble proteins, such as those that originate from inclusion bodies, for example, escherichia coli. we have developed a method that allows the in vitro solubilization of denatured proteins without refolding and their efficient cellular internalization through conjugation to the peptide, octa-arginine (r8), via disulfide bonds with cys ... | 2011 | 22172106 |
| [stable expression of rhvegf165 in chinese hamster ovary cells]. | we established a stable chinese hamster ovary (cho-s) cell line for recombinant human vegf165-expressing. we co-transfected gs-expression vector and rhvegf165 expression plasmid into cho-s cells, and selected the highest vegf165-expressing clone as the working cell line to express vegf165 protein. after 7-day fed-batch culture in a 5 l bioreactor and 3 steps chromatographic purification, we got the rhvegf165 protein for series of binding and biological activity examination. the production was ov ... | 2011 | 22034823 |
| whole-cell electrical activity under direct mechanical stimulus by afm cantilever using planar patch clamp chip approach. | patch clamp is a powerful tool for studying the properties of ion-channels and cellular membrane. in recent years, planar patch clamp chips have been fabricated from various materials including glass, quartz, silicon, silicon nitride, polydimethyl-siloxane (pdms), and silicon dioxide. planar patch clamps have made automation of patch clamp recordings possible. however, most planar patch clamp chips have limitations when used in combination with other techniques. furthermore, the fabrication meth ... | 2011 | 22174731 |
| natb/nat1*4 promotes greater arylamine n-acetyltransferase 1 mediated dna adducts and mutations than nata/nat1*4 following exposure to 4-aminobiphenyl. | n-acetyltransferase 1 (nat1) is a phase ii metabolic enzyme responsible for the biotransformation of aromatic and heterocyclic amine carcinogens such as 4-aminobiphenyl (abp). nat1 catalyzes n-acetylation of arylamines as well as the o-acetylation of n-hydroxylated arylamines. o-acetylation leads to the formation of electrophilic intermediates that result in dna adducts and mutations. nat1 is transcribed from a major promoter, natb, and an alternative promoter, nata, resulting in mrnas with dist ... | 2011 | 21837760 |
| colocalization and regulated physical association of presynaptic serotonin transporters with a3 adenosine receptors. | activation of a(3) adenosine receptors (a(3)ars) rapidly enhances the activity of antidepressant-sensitive serotonin (5-ht) transporters (serts) in vitro, ex vivo, and in vivo. a(3)ar agonist stimulation of sert activity is lost in a(3)ar knockout mice. a(3)ar-stimulated sert activity is mediated by protein kinase g1 (pkgi)- and p38 mitogen-activated protein kinase (mapk)-linked pathways that support, respectively, enhanced sert surface expression and catalytic activation. the mechanisms by whic ... | 2011 | 21705486 |
| stimulating the immune response of litopenaeus vannamei using the phagocytosis activating protein (pap) gene. | high mortality in the shrimp farming industry is caused by several pathogens such as white spot syndrome virus (wssv), yellow head virus (yhv) and vibrio harveyi (v. harveyi). a pap (phagocytosis activating protein) gene able to activate phagocytosis of shrimp hemocytes was cloned into the eukaryotic expression vector phmgfp. in vitro expression was confirmed by transfection of pap-phmgfp into cho (chinese hamster ovary) cells and the expression of the green fluorescent protein (gfp) was observe ... | 2011 | 21699986 |
| Putative role of the adenosine A(3) receptor in the antiproliferative action of N (6)-(2-isopentenyl)adenosine. | We tested a panel of naturally occurring nucleosides for their affinity towards adenosine receptors. Both N (6)-(2-isopentenyl)adenosine (IPA) and racemic zeatin riboside were shown to be selective human adenosine A(3) receptor (hA(3)R) ligands with affinities in the high nanomolar range (K (i) values of 159 and 649-ánM, respectively). These values were comparable to the observed K (i) value of adenosine on hA(3)R, which was 847-ánM in the same radioligand binding assay. IPA also bound with micr ... | 2011 | 21720785 |
| Influence of copper ions on the viability and cytotoxicity of Pseudomonas aeruginosa under conditions relevant to drinking water environments. | Copper plumbing materials can be the source of copper ions in drinking water supplies. The aim of the current study was to investigate the influence of copper ions on the viability and cytotoxicity of the potential pathogen Pseudomonas aeruginosa that presents a health hazard when occurring in building plumbing systems. In batch experiments, exposure of P. aeruginosa (10(6)cells/mL) for 24h at 20-¦C to copper-containing drinking water from domestic plumbing systems resulted in a loss of culturab ... | 2011 | 21742552 |
| enhanced sialylation of recombinant human erythropoietin in chinese hamster ovary cells by combinatorial engineering of selected genes. | therapeutic glycoproteins with exposed galactose (gal) residues are cleared rapidly from the bloodstream by asialoglycoprotein receptors in hepatocytes. various approaches have been used to increase the content of sialic acid, which occupies terminal sites of n- or o-linked glycans and thereby increases the half-life of therapeutic glycoproteins. we enhanced sialylation of human erythropoietin (epo) by genetic engineering of the sialylation pathway in chinese hamster ovary (cho) cells. the enzym ... | 2011 | 21436238 |
| desensitization of transient receptor potential ankyrin 1 (trpa1) by the trp vanilloid 1-selective cannabinoid arachidonoyl-2 chloroethanolamine. | recent studies on cannabinoid-induced analgesia implicate certain transient receptor potential (trp) channels as a therapeutic target along with metabotropic cannabinoid receptors. although trp ankyrin 1 (trpa1)-selective cannabinoids, such as (r)-(+)-[2,3-dihydro-5-methyl-3-(4-morpholinylmethyl) pyrrolo-[1,2,3-d,e]-1,4-benzoxazin-6-yl]-1-naphthalenyl-methanone (win55,212), are effective at desensitizing trpa1 and trp vanilloid 1 (trpv1), there is a gap in knowledge in understanding the opposite ... | 2011 | 21441412 |
| evaluation of process efficiency and bioequivalence of biosimilar recombinant human chorionic gonadotropin (rhcg). | human chorionic gonadotropin (hcg) is a therapeutic protein used for ovulation induction in women with infertility. dong-a pharm. co. has developed recombinant hcg (rhcg) [product code da-3803] produced in chinese hamster ovary cells and evaluated its biologic properties, such as biologic potency, efficacy, and pharmacokinetic profile, compared with a reference product, ovidrel®. | 2011 | 21443274 |
| 4s polycyclic aromatic hydrocarbon receptor (glycine n-methyltransferase) and the aryl hydrocarbon receptor nuclear translocator (hypoxia inducible factor-1ß) interaction in chinese hamster ovary and rat hepatoma cells: 4s pah-r/arnt hetero-oligomers? | rat cyp1a1 promoter-luciferase, transiently transfected wild-type and 4s pah receptor (glycine n-methyl transferase, gnmt)-transformed chinese hamster ovary (cho) cells were exposed to benzo[a]pyrene and assayed for luciferase activity as an indicator of cyp1a1 promoter activity. cho cells transformed with the rat 4s pah receptor/gnmt expression vector had twice the induction level of luciferase activity with respect to wild-type cho cells in concert with previously published reports that the 4s ... | 2011 | 21445860 |
| effectiveness of a chinese hamster ovary cell derived hepatitis b vaccine in chinese rural communities. | to evaluate the effectiveness of a chinese hamster ovary cell derived hepatitis b vaccine in a country community in hebei province, pr china. | 2011 | 21457729 |
| in vivo crystallization of human igg in the endoplasmic reticulum of engineered chinese hamster ovary (cho) cells. | protein synthesis and secretion are essential to cellular life. although secretory activities may vary in different cell types, what determines the maximum secretory capacity is inherently difficult to study. increasing protein synthesis until reaching the limit of secretory capacity is one strategy to address this key issue. under highly optimized growth conditions, recombinant cho cells engineered to produce a model human igg clone started housing rod-shaped crystals in the endoplasmic reticul ... | 2011 | 21464137 |
| novel role of rac1/wave signaling mechanism in regulation of the epithelial na+ channel. | the epithelial na(+) channel (enac) is an essential channel responsible for na(+) reabsorption in the aldosterone-sensitive distal nephron. consequently, enac is a major effector impacting systemic blood volume and pressure. we have shown recently that rac1 increases enac activity, whereas cdc42 fails to change channel activity. here we tested whether rac1 signaling plays a physiological role in modulating enac in native tissue and polarized epithelial cells. we found that rac1 inhibitor nsc2376 ... | 2011 | 21464391 |
| nonsense suppressor therapies rescue peroxisome lipid metabolism and assembly in cells from patients with specific pex gene mutations. | peroxisome biogenesis disorders (pbds) are multisystemic autosomal recessive disorders resulting from mutations in pex genes required for normal peroxisome assembly and metabolic activities. here, we evaluated the potential effectiveness of aminoglycoside g418 (geneticin) and ptc124 (ataluren) nonsense suppression therapies for the treatment of pbd patients with disease-causing nonsense mutations. pbd patient skin fibroblasts producing stable pex2 or pex12 nonsense transcripts and chinese hamste ... | 2011 | 21465523 |
| biochemical and structural study on a s529v mutant acid α-glucosidase responsive to pharmacological chaperones. | recently, pharmacological chaperone therapy for pompe disease with small molecules such as imino sugars has attracted interest. but mutant acid α-glucosidase (gaa) species responsive to imino sugars are limited. to elucidate the characteristics of a mutant gaa responsive to imino sugars, we performed biochemical and structural analyses. among cultured fibroblast cell lines derived from japanese pompe patients, only one carrying p.s529v/p.s619r amino acid substitutions responded to 1-deoxynojirim ... | 2011 | 21471980 |
| enhanced production of human recombinant proteins from cho cells grown to high densities in macroporous microcarriers. | macroporous microcarriers entrap cells in a mesh network allowing growth to high densities and protect them from high shear forces in stirred bioreactor cultures. we report the growth of chinese hamster ovary (cho) cells producing either recombinant human beta-interferon (β-ifn) or recombinant human tissue-plasminogen activator (t-pa) in suspension or embedded in macroporous microcarriers (cytopore 1 or 2). the microcarriers enhanced the volumetric production of both β-ifn and t-pa by up to 2.5 ... | 2011 | 21472499 |
| a simplified universal genomic dna extraction protocol suitable for pcr. | conventional genomic dna extraction protocols need expensive and hazardous reagents for decontamination of phenolic compounds from the extracts and are only suited for certain types of tissue. we developed a simple, time-saving and cost-efficient method for genomic dna extraction from various types of organisms, using relatively innocuous reagents. the protocol employs a single purification step to remove contaminating compounds, using a silica column and a non-hazardous buffer, and a chaotropic ... | 2011 | 21476197 |
| insect cells for antibody production: evaluation of an efficient alternative. | in recent years there has been an increase in both availability and demand for therapeutic monoclonal antibodies. currently, most of these antibodies are produced by stably transfected mammalian cells. in this study we evaluated the use of different baculoviral insect cell systems as an alternative for commonly used production schemes. we expressed the human anti-gp41 antibody 3d6 in spodoptera frugiperda sf9, trichoplusia ni bti-tn5b1-4 "high five", and spodoptera frugiperda sfswt-1 "mimic™(") ... | 2011 | 21477625 |
| assessing the role of the glycosylphosphatidylinositol-anchored high density lipoprotein-binding protein 1 (gpihbp1) three-finger domain in binding lipoprotein lipase. | glycosylphosphatidylinositol-anchored high density lipoprotein-binding protein 1 (gpihbp1) is an endothelial cell protein that transports lipoprotein lipase (lpl) from the subendothelial spaces to the capillary lumen. gpihbp1 contains two main structural motifs, an amino-terminal acidic domain enriched in aspartates and glutamates and a lymphocyte antigen 6 (ly6) motif containing 10 cysteines. all of the cysteines in the ly6 domain are disulfide-bonded, causing the protein to assume a three-fing ... | 2011 | 21478160 |
| a parallel microfluidic flow cytometer for high-content screening. | a parallel microfluidic cytometer (pmc) uses a high-speed scanning photomultiplier-based detector to combine low-pixel-count, one-dimensional imaging with flow cytometry. the 384 parallel flow channels of the pmc decouple count rate from signal-to-noise ratio. using six-pixel one-dimensional images, we investigated protein localization in a yeast model for human protein misfolding diseases and demonstrated the feasibility of a nuclear-translocation assay in chinese hamster ovary (cho) cells expr ... | 2011 | 21478861 |
| oxaliplatin elicits mechanical and cold allodynia in rodents via trpa1 receptor stimulation. | platinum-based anticancer drugs cause neurotoxicity. in particular, oxaliplatin produces early-developing, painful, and cold-exacerbated paresthesias. however, the mechanism underlying these bothersome and dose-limiting adverse effects is unknown. we hypothesized that the transient receptor potential ankyrin 1 (trpa1), a cation channel activated by oxidative stress and cold temperature, contributes to mechanical and cold hypersensitivity caused by oxaliplatin and cisplatin. oxaliplatin and cispl ... | 2011 | 21481532 |
| therapeutic potential of intracerebroventricular replacement of modified human β-hexosaminidase b for gm2 gangliosidosis. | to develop a novel enzyme replacement therapy for neurodegenerative tay-sachs disease (tsd) and sandhoff disease (sd), which are caused by deficiency of β-hexosaminidase (hex) a, we designed a genetically engineered hexb encoding the chimeric human β-subunit containing partial amino acid sequence of the α-subunit by structure-based homology modeling. we succeeded in producing the modified hexb by a chinese hamster ovary (cho) cell line stably expressing the chimeric hexb, which can degrade artif ... | 2011 | 21487393 |
| a novel kcnj2 nonsense mutation, s369x, impedes trafficking and causes a limited form of andersen-tawil syndrome. | background: -mutations in kcnj2, a gene encoding the inward rectifier k(+) channel kir2.1, are associated with andersen-tawil syndrome (ats), which is characterized by (1) ventricular tachyarrhythmias associated with qt (qu) interval prolongation, (2) periodic paralysis, and (3) dysmorphic features. methods and results: -we identified a novel kcnj2 mutation, s369x, in a 13-year-old boy with prominent qu interval prolongation and mild periodic paralysis. the mutation results in the truncation at ... | 2011 | 21493816 |
| affinity chromatography based on a combinatorial strategy for rerythropoietin purification. | small peptides containing fewer than 10 amino acids are promising ligand candidates with which to build affinity chromatographic systems for industrial protein purification. the application of combinatorial peptide synthesis strategies greatly facilitates the discovery of suitable ligands for any given protein of interest. here we sought to identify peptide ligands with affinity for recombinant human erythropoietin (rhepo), which is used for the treatment of anemia. a combinatorial library conta ... | 2011 | 21495625 |
| extracellular purine and pyrimidine catabolism in cell culture. | the presence of purines and pyrimidines bases, nucleosides, and nucleotides in the culture medium has shown to differently affect the growth of a chinese hamster ovary (cho) cell line producing the secreted form of the human placental alkaline phosphatase enzyme (seap; carvalhal et al., biotech prog. 2003;19:69-83). cho, bhk, as well as sf9 cell growth was clearly reduced in the presence of purines but was not affected by pyrimidines at the concentrations tested. the knowledge about the mechanis ... | 2011 | 21695809 |
| in vitro pharmacology of aripiprazole, its metabolite and experimental dopamine partial agonists at human dopamine d(2) and d(3) receptors. | aripiprazole is the first dopamine d(2)/d(3) receptor partial agonist successfully developed and ultimately approved for treatment of a broad spectrum of psychiatric and neurological disorders. aripiprazole's dopamine d(2) and serotonin 5-ht(1a) receptor partial agonist activities have been postulated to confer clinical efficacy without marked sedation, and a relatively favorable overall side-effect profile. using aripiprazole's unique profile as a benchmark for new dopamine partial agonist deve ... | 2011 | 21816144 |
| centromere conversion and retention in somatic cell hybrids. | the generation of somatic cell hybridization-derived cell lines between highly divergent species affords the opportunity to examine the concept of 'genome dominance' in the context of genetic and epigenetic changes. while whole-scale genome dominance has been well documented in natural hybrids among closely related species, an examination of centromere position and sequence retention in 2 marsupial-eutherian hybrids has revealed a mechanism for 'centromere dominance' as a driving force in the ge ... | 2011 | 21709412 |
| sodium fluorescein is a probe substrate for hepatic drug transport mediated by oatp1b1 and oatp1b3. | the aim of this study was to characterize the in vitro hepatic uptake kinetics of sodium fluorescein (nafluo) and identify the transporters involved. nafluo exhibited saturable uptake kinetics in suspended rat and human hepatocytes as reflected by k(m) values of 22.5 and 14.1 -ám, and v(max) values of 98.3 and 5.8 pmol/(million cells ôçó min), respectively. coincubation with known inhibitors (e.g. rifampicin) of organic anion transporting polypeptide (oatp/oatp; slco gene family) significantly d ... | 2011 | 21837650 |
| studies on the cytotoxic, apoptotic and antitumoral effects of au(iii) and pt(ii) complexes of 1, 10-phenanthroline on v79 379a and a549 cell lines. | in the present study, au(iii) and pt(ii) complexes of 1, 10-phenanthroline (phen) were synthesized and used as the test compounds. the structure elucidation of the synthesized compounds was performed by ir, (1)h-nmr and mass spectroscopic data and the results of elemental analyses. the cytotoxic and apoptotic effects of test compounds were elucidated on v79 379a (chinese hamster lung fibroblast like) and a549 (human lung carcinoma epithelial like) cell lines. cytotoxicity was measured with mtt a ... | 2011 | 21815775 |
| protein kinase c downregulates i(ks) by stimulating kcnq1-kcne1 potassium channel endocytosis. | background: the slow-activating cardiac repolarization k(+) current (i(ks)), generated by the kcnq1-kcne1 potassium channel complex, is controlled via sympathetic and parasympathetic regulation in vivo. inherited kcnq1 and kcne1 mutations predispose to ventricular fibrillation and sudden death, often triggered by exercise or emotional stress. protein kinase c (pkc), which is activated by +¦1 adrenergic receptor stimulation, is known to downregulate i(ks) via phosphorylation of kcne1 serine 102, ... | 2011 | 21699843 |
| in vitro and in vivo assessment of the genotoxic activity of aloesin. | aloesin is a chromone that is a component of aloe spp. it may have potential as a functional food ingredient as it has been shown to likely have beneficial effects in persons in a pre-diabetic state or who have metabolic syndrome. in this study the safety of aloesin has been evaluated using a series of in vitro and in vivo genotoxicity assays including, bacterial mutation, mammalian cell cytogenetic, and mouse micronucleus tests. aloesin did not induce reverse mutations in salmonella typhimurium ... | 2011 | 21821088 |
| chromosome transfer via cell fusion. | intact chromosomes as well as chromosome fragments can be vehicled into various recipient cells without perturbing their ability to segregate as free elements; chromosome transfer can be performed both in cultured cells and in living animals. the method of choice to shuttle single chromosomes between cells is microcell fusion named microcell mediated chromosome transfer (mmct). the use of mmct is mandatory in a number of applications where alternative chromosome transfection procedures are ineff ... | 2011 | 21431719 |
| mutagenic, cytotoxic, and genotoxic properties of tobacco smoke produced by cigarillos available on the canadian market. | cigarillos (aka little cigars) have been increasing in popularity unlike cigarettes; but relatively little is known about the toxicology of the mainstream smoke (mss) from such products. therefore, the objective of this work was to compare the toxicological properties of the mss (health canada intensive smoking conditions) from a range of cigarillo products with the toxicological properties of mss of cigarettes. three in vitro assays were used to evaluate the toxicities of the mss total particul ... | 2011 | 21821091 |
| expression and localization of the 20+¦-hydroxysteroid dehydrogenase (hsd) enzyme in the reproductive tissues of the cynomolgus monkey macaca fascicularis. | this study was conducted to characterize and functionally analyze the monkey 20+¦-hydroxysteroid dehydrogenase (20+¦-hsd) in the ovary, placenta, and oviduct. we focused on 20+¦-hsd mrna expression and protein localization in monkey reproductive tissues and the molecular characterization of the promoter region. reverse transcription-polymerase chain reaction (rt-pcr) monkey 20+¦-hsd mrna was more strongly detected in the ovary at pre-ovulation than in the placenta and oviduct at pre-parturition. ... | 2011 | 21831350 |
| cytotoxic cardenolide and sterols from calotropis gigantea. | the dichloromethane extract from the leaves of calotropis gigantea linn. was strongly cytotoxic against non-small cell lung carcinoma (a549), colon carcinoma (hct 116) and hepatocellular carcinoma (hep g2), and non toxic to chinese hamster ovary (aa8). the extract afforded uscharin (1), 3,5,8-trihydroxy-24-methylcholest-6,22-diene (2), a mixture of (24r)-3beta-hydroxy-24-ethylcholest-5-en-7-one (3a) and 6beta-hydroxy-24-ethylcholest-4,22-dien-3-one (3b), and another mixture of (24r)-24-ethylchol ... | 2011 | 21815415 |
| a high-content drug screen identifies ursolic acid as an inhibitor of amyloid-{beta} interactions with its receptor cd36. | a pathological hallmark of alzheimer's disease (ad) is deposition of amyloid-+¦ (a+¦) in the brain. a+¦ binds to microglia via a receptor complex that includes cd36 leading to production of pro-inflammatory cytokines and neurotoxic reactive oxygen species (ros), and subsequent neurodegeneration. interruption of a+¦ binding to cd36 is a potential therapeutic strategy for ad. to identify pharmacologic inhibitors of a+¦ binding to cd36, we developed a 384-well plate assay for binding of fluorescent ... | 2011 | 21835916 |
| pharmacokinetics and ex vivo whole blood clot formation of a new recombinant fviii (n8) in haemophilia a dogs. | summary.ôçé n8, a new recombinant factor viii (rfviii) compound developed for the treatment of haemophilia a, is produced in chinese hamster ovary (cho) cells and formulated without human- or animal-derived materials. the aim of the present study was to compare the pharmacokinetics (pk) and the procoagulant effect, measured by ex vivo whole blood clot formation, of n8 and a commercial rfviii in a cross-over study in haemophilia a dogs. n8 and advate(-«) (100ôçâiuôçâkg(-1) ) were administered int ... | 2011 | 21682818 |
| in vitro and in vivo genotoxic effects of somatic cell nuclear transfer cloned cattle meat. | although the nutritional composition and health status after consumption of the meat and milk derived from both conventionally bred (normal) and somatic cell nuclear transferred (cloned) animals and their progeny are not different, little is known about their food safeties like genetic toxicity. this study is performed to examine both in vitro (bacterial mutation and chromosome aberration) and in vivo (micronucleus) genotoxicity studies of cloned cattle meat. the concentrations of both normal an ... | 2011 | 21722695 |
| renal organic cation transporters mediated cadmium-induced nephrotoxicity. | the involvement of renal organic cation transporters (octs) in cadmium transport was investigated in chinese hamster ovary (cho-k1) cells stably and singly transfected with rabbit (rb)oct1 and rboct2, in murine isolated renal proximal tubule, and in intact kidney following bilateral ureteral ligation of rat. cadmium inhibited uptake of [(3)h]-tetraethylammonium (tea), a substrate of rboct1 and rboct2, via both transporters with half maximal inhibitory concentration (ic(50)) of cadmium for rboct1 ... | 2011 | 21513783 |
| regulation of pi3k/akt signaling by n-desmethylclozapine through activation of δ-opioid receptor. | we have previously reported that n-desmethylclozapine (ndmc), a major clozapine metabolite, acts as a δ-opioid receptor agonist. here, we show that in different cellular systems ndmc regulates protein kinase b/akt (akt) signaling through the activation of δ-opioid receptors. in chinese hamster ovary cells transfected with the human δ-opioid receptor (cho/dor), ndmc induced a time- and concentration-dependent phosphorylation of akt at thr308 and glycogen synthase kinase-3β (gsk-3β) at ser9 and th ... | 2011 | 21515255 |
| impact of site-specific n-glycosylation on cellular secretion, activity and specific activity of the plasma phospholipid transfer protein. | the plasma phospholipid transfer protein (pltp) plays a key role in lipid and lipoprotein metabolism. it has six potential n-glycosylation sites. to study the impact of these sites on pltp secretion and activity, six variants containing serine to alanine point mutations were prepared by site-directed mutagenesis and expressed in chinese hamster ovary flp-in cells. the apparent size of each of the six pltp mutants was slightly less than that of wild type by western blot, indicating that all six s ... | 2011 | 21515415 |
| ras/mapk signalling modulates protein stability and cell surface expression of sr-bi. | the mitogen-activated protein kinase (mapk) erk1/2 has been implicated to modulate the activity of nuclear receptors, including peroxisome proliferator activator receptors (ppars) and liver x receptor (lxr), to alter the ability of cells to export cholesterol. here we investigated if the ras-raf-mek-erk1/2 signalling cascade could affect reverse cholesterol transport via modulation of scavenger receptor bi (sr-bi) levels. we demonstrate that in chinese hamster ovary (cho) and human embryonic kid ... | 2011 | 21525007 |
| chemical toxicity testing in vitro using cytochrome p450-expressing cell lines, such as human cyp1b1. | this protocol describes how to use cytochrome p450-dependent monooxygenase (cyp)-expressing cell lines in toxicity testing of chemicals in vitro. selected cells amenable to permanently grow in culture are genetically manipulated to stably express single cyp enzymes originating from any species of interest. this expression can be characterized by, for example, determining cyp mrna content, cyp protein level (western blotting or in situ immunofluorescence) and cyp-mediated enzyme activity (substra ... | 2011 | 21527924 |
| analysis of repetitive dna in chromosomes by flow cytometry. | we developed a flow cytometry method, chromosome flow fluorescence in situ hybridization (fish), called cff, to analyze repetitive dna in chromosomes using fish with directly labeled peptide nucleic acid (pna) probes. we used cff to measure the abundance of interstitial telomeric sequences in chinese hamster chromosomes and major satellite sequences in mouse chromosomes. using cff we also identified parental homologs of human chromosome 18 with different amounts of repetitive dna. | 2011 | 21532581 |
| recombinant glycoprotein e produced in mammalian cells in large-scale as an antigen for varicella-zoster-virus serology. | a recombinant glycoprotein e (ge) from varicella-zoster virus (vzv) was generated and produced in chinese hamster ovary (cho) cells, in the development of a specific antigen for analysis of igg antibodies to vzv. several stable ge-secreting clones were established and one clone was adapted to growth in serum-free suspension culture. when the cells were cultured in a perfusion bioreactor, ge was secreted into the medium, from where it could be easily purified. the recombinant ge was then evaluate ... | 2011 | 21540058 |
| development and characterization of a chinese hamster ovary cell-specific oligonucleotide microarray. | the chinese hamster ovary (cho) cell line is one of the most widely used mammalian cell lines for biopharmaceutical production. we have developed and characterized a gene expression microarray (wyehamster2a) specific for cho cells that has enabled the study of ~3,500 sequences. analysis of multiple sets of replicate scans showed that data derived from the wyehamster2a array is highly reproducible confirming it as a robust tool for profiling. twelve gene sequences were selected for follow-up rt-q ... | 2011 | 21547514 |
| a dual reporter cell assay for identifying serotype and drug susceptibility of herpes simplex virus. | a dual reporter cell assay (drca) that allows real-time detection of herpes simplex virus (hsv) infection was developed. this was achieved by stable transfection of cells with an expression cassette that contains the dual reporter genes, secreted alkaline phosphatase (seap) and enhanced green fluorescent protein (egfp), under the control of an hsv early gene promoter. baby hamster kidney (bhk) and chinese hamster ovary (cho) cell lines were used as parental cell lines because the former is permi ... | 2011 | 21549682 |
| comparative analysis of micronuclei and dna damage induced by ochratoxin a in two mammalian cell lines. | the fungal toxin, ochratoxin a (ota), is a common contaminant in human food and animal feed. the present study evaluated micronucleus (mn) induction by ota in comparison with its ability to induce cytotoxicity and dna damage in two mammalian cell lines, cho-k1-bh(4) chinese hamster ovary cells and tk6 human lymphoblastoid cells. micronuclei were evaluated by flow cytometry, cytotoxicity was estimated by relative population doubling (rpd), while direct dna damage and oxidative dna damage were mea ... | 2011 | 21554981 |
| variation in missegregation rates among human chromosomes in hybrid cells. | chinese hamster ovary-human hybrid cells containing chromosomes 16, 18, x, or 21 have been used to test the ability of human kinetochores to successfully bind to spindle microtubules and to be distributed to daughter cells. the intrinsic rates of non-disjunction among these human chromosomes have been determined and compared to non-disjunction rates in cells presented with taxol as a mitotic challenge. cells grown on culture slides were fixed and processed for immunofluorescence and fluorescence ... | 2011 | 21555874 |
| [construction and expression of the eukaryotic expression vector carrying hsv-1 gc glycoprotein gene.] | aim: to stably express herpes simplex virus type 1 (hsv-1) glycoprotein c (gc) in chinese hamster ovary cells (cho-k1). methods: the eukaryotic expression vector pci-mcmv-gc-1-ires-dhfr-l22r was constructed and transfected into cho-k1 cells by lipofectamine(tm); 2000. the transfected cells were selected by g418 and methotrexate (mtx). the expression of hsv-1 gc was analyzed by slot blot. hsv-1 gc proteins were purified with his-ni sepharose and then detected by western blot. results: the eukaryo ... | 2011 | 21557903 |
| dinapinones, novel inhibitors of triacylglycerol synthesis in mammalian cells, produced by penicillium pinophilum fki-3864. | dinapinone a, a novel biaryl dihydronaphthopyranone, was isolated from the culture broth of penicillium pinophilum fki-3864 by solvent extraction, silica gel and ods column chromatography and hplc. dinapinone a showed very potent inhibition of triacylglycerol (tg) synthesis in intact chinese hamster ovary k1 (cho-k1) cells with an ic(50) value of 0.097 μm. dinapinone a was found to be a mixture of stereoisomers, resulting in its separation into dinapinones a1 and a2 by hplc using a c30 reverse-p ... | 2011 | 21559025 |
| cell type-specific and site directed n-glycosylation pattern of fcγriiia. | human leukocyte receptor iiia (hfcγriiia) plays a prominent role in the elimination of tumor cells by antibody-based cancer therapies. in previous studies, a major impact of the presence of carbohydrates at asn-162 on the binding between the receptor and the fc part of wild type fucosylated or glycoengineered nonfucosylated antibodies has been shown. in this study, we performed a site directed carbohydrate analysis at hfcγriiia derived from human embryonic kidney (hek) and chinese hamster ovary ... | 2011 | 21561106 |
| ionflux: a microfluidic patch clamp system evaluated with human ether-à-go-go related gene channel physiology and pharmacology. | abstract ion channel assays are essential in drug discovery, not only for identifying promising new clinical compounds, but also for minimizing the likelihood of potential side effects. both applications demand optimized throughput, cost, and predictive accuracy of measured membrane current changes evoked or modulated by drug candidates. several competing electrophysiological technologies are available to address this demand, but important gaps remain. we describe the industrial application of a ... | 2011 | 21561375 |
| akap79/150 signal complexes in g-protein modulation of neuronal ion channels. | voltage-gated m-type (kcnq) k(+) channels play critical roles in regulation of neuronal excitability. previous work showed a-kinase-anchoring protein (akap)79/150-mediated protein kinase c (pkc) phosphorylation of m channels to be involved in m current (i(m)) suppression by muscarinic m(1), but not bradykinin b(2), receptors. in this study, we first explored whether purinergic and angiotensin suppression of i(m) in superior cervical ganglion (scg) sympathetic neurons involves akap79/150. transfe ... | 2011 | 21562284 |
| identification of a novel temperature sensitive promoter in cho cells. | abstract: background: the chinese hamster ovary (cho) expression system is the leading production platform for manufacturing biopharmaceuticals for the treatment of numerous human diseases. efforts to optimize the production process also include the genetic construct encoding the therapeutic gene. here we report about the successful identification of an endogenous highly active gene promoter obtained from cho cells which shows conditionally inducible gene expression at reduced temperature. resul ... | 2011 | 21569433 |
| akap150-mediated trpv1 sensitization is disrupted by calcium/calmodulin. | abstract: background: the transient receptor potential vanilloid type1 (trpv1) is expressed in nociceptive sensory neurons and is sensitive to phosphorylation. a-kinase anchoring protein 79/150 (akap150) mediates phosphorylation of trpv1 by protein kinases a and c, modulating channel activity. however, few studies have focused on the regulatory mechanisms that control akap150 association with trpv1. in the present study, we identify a role for calcium/calmodulin in controlling akap150 associatio ... | 2011 | 21569553 |
| fancd1/brca2 plays predominant role in the repair of dna damage induced by acnu or tmz. | nimustine (acnu) and temozolomide (tmz) are dna alkylating agents which are commonly used in chemotherapy for glioblastomas. acnu is a dna cross-linking agent and tmz is a methylating agent. the therapeutic efficacy of these agents is limited by the development of resistance. in this work, the role of the fanconi anemia (fa) repair pathway for dna damage induced by acnu or tmz was examined. cultured mouse embryonic fibroblasts were used: fanca(-/-), fancc(-/-), fanca(-/-)c(-/-), fancd2(-/-) cell ... | 2011 | 21573016 |
| production and characterization of chimeric monoclonal antibodies against burkholderia pseudomallei and b. mallei using the dhfr expression system. | burkholderia pseudomallei (bp) and b. mallei (bm) are closely related gram-negative, facultative anaerobic bacteria which cause life-threatening melioidosis in human and glanders in horse, respectively. our laboratory has previously generated and characterized more than 100 mouse monoclonal antibodies (mabs) against bp and bm, according to in vitro and in vivo assay. in this study, 3 mabs (bp7 10b11, bp7 2c6, and bp1 7f7) were selected to develop into chimeric mouse-human monoclonal antibodies ( ... | 2011 | 21573027 |
| n-glycosylation profiling of recombinant mouse extracellular superoxide dismutase produced in chinese hamster ovary cells. | extracellular superoxide dismutase (ec-sod), the major sod isoenzyme in biological fluids, is known to be n-glycosylated and heterogeneous as was detected in most glycoproteins. however, only one n-glycan structure has been reported in recombinant human ec-sod produced in chinese hamster ovary (cho) cells. thus, a precise n-glycan profile of the recombinant ec-sod is not available. in this study, we report profiling of the n-glycan in the recombinant mouse ec-sod produced in cho cells using high ... | 2011 | 21573946 |
| microvesicle-mediated release of soluble lh/hcg receptor (lhcgr) from transfected cells and placenta explants. | abstract: placental hcg and pitutary lh transduce signals in target tissues through a common receptor (lhcgr). we demonstrate that recombinant lhcgr proteins which include the hormone-binding domain are secreted from transfected cells and that natural lhcgr is also secreted from human placental explants. lhcgr recombinant proteins representing varying lengths of the n-terminal extracellular domain were expressed in chinese hamster ovary cells in suspension culture. secretion was minimal up to 72 ... | 2011 | 21575145 |
| evaluation of genotoxicity of antrodia cinnamomea in the ames test and the in vitro chromosomal aberration test. | antrodia cinnamomea is an expensive and highly valued folk medicinal fungus that grows only inside the rotten trunk of cinnamomum kanehirae, an evergreen broad-leaved tree. this fungus has recently been used commercially in the formulation of nutraceuticals and functional foods in taiwan. it has been used for centuries as a detoxificant in cases of food poisoning, diarrhea, vomiting, hepatic disease and various kinds of cancers. the present study investigated the effects of antrodia cinnamomea o ... | 2011 | 21576417 |
| activation of kcnq2/3 potassium channels by novel pyrazolo[1,5-a]pyrimidin-7(4h)-one derivatives. | the voltage-gated m-type potassium channel, encoded mainly by the kcnq2/3 genes, plays an important role in the control of neuronal excitability. mutations in the kcnq2 gene lead to a form of neonatal epilepsy in humans termed 'benign familial neonatal convulsions', which is characterized by hyperexcitability of neurons. kcnq openers or activators are expected to decrease the firing of overactive neurons and are thus conducive to the treatment of epilepsy and pain. here, we report that four nove ... | 2011 | 21577044 |
| demonstration that endoplasmic reticulum-associated degradation of glycoproteins can occur downstream of processing by endomannosidase. | during quality control in the endoplasmic reticulum (er), nascent glycoproteins are deglucosylated by er glucosidases i and ii. in the post-er compartments, glycoprotein endo-α-mannosidase provides an alternative route for deglucosylation. previous evidence suggests that endomannosidase non-selectively deglucosylates glycoproteins escaping quality control in the endoplasmic reticulum, facilitating secretion of aberrantly folded as well as normal glycoproteins. here, we employ free oligosaccharid ... | 2011 | 21585340 |
| molecular characterization of chinese hamster cells mutants affected in adenosine kinase and showing novel genetic and biochemical characteristics. | abstract: background: two isoforms of the enzyme adenosine kinase (adk), which differ at their n-terminal ends, are found in mammalian cells. however, there is no information available regarding the unique functional aspects or regulation of these isoforms. results: we show that the two adk isoforms differ only in their first exons and the promoter regions; hence they arise via differential splicing of their first exons with the other exons common to both isoforms. the expression of these isofor ... | 2011 | 21586167 |
| th-030418: a potent long-acting opioid analgesic with low dependence liability. | numerous efforts have been made on the chemical modification of opioid compounds, with the ultimate goal of developing new opioid analgesics that is highly potent and low/non-addictive. in a search for such compounds, th-030418 [7α-[(r)-1-hydroxy-1-methyl-3-(thien-3-yl)-propyl]-6,14-endo-ethanotetrahydrooripavine] was synthesized. here, we evaluated the pharmacological activities of th-030418, in comparison with morphine, the prototype opioid analgesic. in radioligand binding assays, th-030418 b ... | 2011 | 21594658 |
| dendronized polyaniline nanotubes for cardiac tissue engineering. | today, nanobiomaterials represent a very important class of biomaterials because they differ dramatically in their bulk precursors. the properties of these materials are determined by the size and morphology, thus creating a fascinating line in their physicochemical properties. polyaniline nanotubes (panints) are one of the most promising nanobiomaterials for cardiac tissue engineering applications due to their electroactive properties. the biocompatibility and low hydrophilic properties of pani ... | 2011 | 21595714 |
| clinical and molecular characterization of a transmitted reciprocal translocation t(1;12)(p32.1;q21.3) in a family co-segregating with mental retardation, language delay, and microcephaly. | abstract: background: chromosome translocation associated with neurodevelopmental disorders provides an opportunity to identify new disease-associated genes and gain new insight into their function. during chromosome analysis, we identified a reciprocal translocation between chromosomes 1p and 12q, t(1; 12)(p32.1; q21.3), co-segregating with microcephaly, language delay, and severe psychomotor retardation in a mother and her two affected boys. methods: fluorescence in situ hybridization (fish), ... | 2011 | 21595979 |