Publications

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the molecular and immunochemical expression of innexins in the yellow fever mosquito, aedes aegypti: insights into putative life stage- and tissue-specific functions of gap junctions.gap junctions (gj) mediate direct intercellular communication by forming channels through which certain small molecules and/or ions can pass. connexins, the proteins that form vertebrate gj, are well studied and known to contribute to neuronal, muscular and epithelial physiology. innexins, the gj proteins of insects, have only recently received much investigative attention and many of their physiological roles remain to be determined. here we characterize the molecular expression of six innexin ...201525585357
annotation and expression analysis of cuticular proteins from the tobacco hornworm, manduca sexta.the insect cuticle is a unique material that covers the exterior of the animal as well as lining the foregut, hindgut, and tracheae. it offers protection from predators and desiccation, defines body shape, and serves as an attachment site for internal organs and muscle. it has demonstrated remarkable variations in hardness, flexibility and elasticity, all the while being light weight, which allows for ease of movement and flight. it is composed primarily of chitin, proteins, catecholamines, and ...201525576653
tsetse gmmsrpn10 has anti-complement activity and is important for successful establishment of trypanosome infections in the fly midgut.the complement cascade in mammalian blood can damage the alimentary tract of haematophagous arthropods. as such, these animals have evolved their own repertoire of complement-inactivating factors, which are inadvertently exploited by blood-borne pathogens to escape complement lysis. unlike the bloodstream stages, the procyclic (insect) stage of trypanosoma brucei is highly susceptible to complement killing, which is puzzling considering that a tsetse takes a bloodmeal every 2-4 days. in this stu ...201525569180
pyrethroid-resistance and presence of two knockdown resistance (kdr) mutations, f1534c and a novel mutation t1520i, in indian aedes aegypti.control of aedes aegypti, the mosquito vector of dengue, chikungunya and yellow fever, is a challenging task. pyrethroid insecticides have emerged as a preferred choice for vector control but are threatened by the emergence of resistance. the present study reports a focus of pyrethroid resistance and presence of two kdr mutations--f1534c and a novel mutation t1520i, in ae. aegypti from delhi, india.201525569164
origins of context-dependent gene repression by capicua.receptor tyrosine kinase (rtk) signaling pathways induce multiple biological responses, often by regulating the expression of downstream genes. the hmg-box protein capicua (cic) is a transcriptional repressor that is downregulated in response to rtk signaling, thereby enabling rtk-dependent induction of cic targets. in both drosophila and mammals, cic is expressed as two isoforms, long (cic-l) and short (cic-s), whose functional significance and mechanism of action are not well understood. here ...201525569482
engineered mosquitoes to fight mosquito borne diseases: not a merely technical issue.malaria, dengue and other mosquito-borne diseases pose dramatic problems of public health, particularly in tropical and sub-tropical countries. historically, vector control has been one of the most successfully strategies to eradicate some mosquito-borne diseases, as witnessed by malaria eradication in mediterranean regions such as italy and greece. vector control through insecticides has been used worldwide; unfortunately, it is losing effectiveness due to spread of resistances. control of mosq ...201525495663
dengue fever in china: an emerging problem demands attention. 201526038760
trade-off between thermal tolerance and insecticide resistance in plutella xylostella.fitness costs associated with resistance to insecticides have been well documented, usually at normal temperature conditions, in many insect species. in this study, using chlorpyrifos-resistant homozygote (rr) and chlorpyrifos-susceptible homozygote (ss) of resistance ace1 allele of plutella xylostella (dbm), we confirmed firstly that high temperature experience in pupal stage influenced phenotype of wing venation in insecticide-resistant and insecticide-susceptible plutella xylostella, and ss d ...201525691976
[competition between aedes aegypti and aedes albopictus larvae in the laboratory].this study had the aim of evaluating the effects of intra and interspecies larval competition between aedes aegypti and aedes albopictus, survival of larvae time taken to develop and wing length. the experiment was carried out with three densities and five proportions of the species. aedes aegypti survival was greater than aedes albopictus survival at intermediate density, while it was lower at high density. only the time taken for aedes albopictus to develop was affected. the differences found ...201519009190
[epidemics and disease during the revolution period in mexico].the health condition in mexico was bad around de beginning of the revolutionary period. the movement of troops led the development of epidemics like yellow fever, typhus, smallpox, and influenza that were enhance with natural disasters and hunger in whole country, from cost to cost and in the north big cities like monterrey, guadalajara and saltillo. doctor liceaga conducted a well planned campaign against yellow fever eradicating water stagnant deposits in order to combat the vector transmissio ...201520929620
[prevalence of reactions secundary to mosquito bites aedes aegypti at en el regional center of allergy and clinical immunology, university hospital, de monterrey, nuevo leon].although systemic reactions resulting from hymenoptera stings have been studied extensively, the prevalence of allergic reactions to mosquitoes is unknown.201520857628
travel-related mosquito-transmitted disease questionnaire survey among health professionals in taiwan.health-care professionals can help travelers by providing accurate pre-travel counseling for mosquito-transmitted diseases such as malaria, yellow fever, and dengue fever. governments and international organizations will benefit from knowledge survey among health professionals in this field to promote the development of travel health profession. this study investigates physicians' and nurses' knowledge regarding malaria, yellow fever, and dengue fever.201521199140
comparison among homemade repellents made with cloves, picaridin, andiroba, and soybean oil against aedes aegypti bites. 201522231260
[predatory capacity of macrobrachium tenellum on aedes aegypti larvae in lab conditions].in the last few years, a lot of importance has been given to natural predators against aedes aegypti. several organisms have been studied both in lab and in the field so as to find out their capacity to devour mosquito larvae. high densities of macrobrachium tenellum are found in natural conditions, it is not aggressive and may stand wide ranges of temperature, rates of salinity and oxygen concentrations.201523424807
synthesis of silver nanoparticles from azadirachta indica--a most effective method for mosquito control.mosquitoes transmit major communicable diseases such as dengue, malaria, filariasis, japanese encephalitis, chikungunya, and so on. vector control is important in epidemic disease situations as there is an urgent need to develop new and improved mosquito control methods that are economical and effective yet safe for non-targeted organisms. in the present study, silver nanoparticles (agnps) were synthesized from the aqueous leaf extract of neem plant (azadirachta indica), and their effects on mos ...201525226837
social sustainability of mesocyclops biological control for dengue in south vietnam.copepod mesocyclops as biological control agents for dengue was previously proven to be effective and sustainable in the northern and central provinces of vietnam. we aim to study social sustainability of mesocyclops intervention in south vietnam. both quantitative and qualitative approaches were used. an entomological survey was carried out in 100 random households of chanh an commune, vinh long province. aedes larval indices and mesocyclops prevalence were compared with historical pre- and pos ...201525312335
a model for the development of aedes (stegomyia) aegypti as a function of the available food.we discuss the preimaginal development of the mosquito aedes aegypti from the point of view of the statistics of developmental times and the final body-size of the pupae and adults. we begin the discussion studying existing models in relation to published data for the mosquito. the data suggest a developmental process that is described by exponentially distributed random times. the existing data show as well that the idea of cohorts emerging synchronously is verified only in optimal situations c ...201525451964
chitin is a necessary component to maintain the barrier function of the peritrophic matrix in the insect midgut.in most insects, the peritrophic matrix (pm) partitions the midgut into different digestive compartments, and functions as a protective barrier against abrasive particles and microbial infections. in a previous study we demonstrated that certain pm proteins are essential in maintaining the pm's barrier function and establishing a gradient of pm permeability from the anterior to the posterior part of the midgut which facilitates digestion (agrawal et al., 2014). in this study, we focused on the e ...201525449129
efficacy of thai herbal essential oils as green repellent against mosquito vectors.repellency activity of thai essential oils derived from ylang ylang (cananga odorata (lamk.) hook.f. & thomson: annonaceae) and lemongrass (cymbopogon citratus (dc.) stapf: poaceae) were tested against two mosquito vectors, aedes aegypti (l.) and culex quinquefasciatus (say). there were compared with two chemical repellents (deet 20% w/w; sketolene shield(®) and ir3535, ethyl butylacetylaminopropionate 12.5% w/w; johnson's baby clear lotion anti-mosquito(®)). each herbal repellent was applied in ...201525438256
the larvicidal activity of agave sisalana against l4 larvae of aedes aegypti is mediated by internal necrosis and inhibition of nitric oxide production.dengue is a viral disease that affects about 50 million people per year around the world. the aim of this study was to investigate the larvicidal activity of agave sisalana crude extract in order to develop a new insecticide against aedes aegypti. in larvicidal activity assays, fourth-stage ae. aegypti larvae were exposed to different concentrations of a. sisalana crude extract for 3, 6, 12, and 24 h for determining the lc50. next, we explored its cytotoxic activity by flow cytometry. furthermor ...201525395257
dengue outbreaks in divinopolis, south-eastern brazil and the geographic and climatic distribution of aedes albopictus and aedes aegypti in 2011-2012.to entomologically monitor aedes spp. and correlate the presence of these vectors with the recent epidemic of dengue in divinopolis, minas gerais state, brazil.201525328988
adulticidal and smoke toxicity of cipadessa baccifera (roth) plant extracts against anopheles stephensi, aedes aegypti, and culex quinquefasciatus.mosquito vectors are responsible for the transmission of parasitic and viral infections, including loss in commercial and labor outputs, particularly in developing countries with tropical and subtropical climates. the aim of the present study is to evaluate the adulticidal and smoke toxicity of cipadessa baccifera (roth) against three important mosquitoes vectors, anopheles stephensi, aedes aegypti, and culex quinquefasciatus (diptera: culicidae). adult mortality was observed after 24-h recovery ...201525320044
screening of rubiaceae and apocynaceae extracts for mosquito larvicidal potential.rubiaceae and apocynaceae families are well known for the expression of cyclotides having insecticidal properties. leaves and flowers extracts of plants from the families rubiaceae (ixora coccinea) and apocynaceae (allamanda violacea) were evaluated for mosquito larvicidal effect against early ivth instars of aedes aegypti and anopheles stephensi. two forms of plant extracts, one untreated and the other treated with heat and proteolytic enzyme were used for assay. after primary assay, the extrac ...201525317964
aedes aegypti larvicide from the ethanolic extract of piper nigrum black peppercorns.due to unavailability of a vaccine and a specific cure to dengue, the focus nowadays is to develop an effective vector control method against the female aedes aegypti mosquito. this study aims to determine the larvicidal fractions from piper nigrum ethanolic extracts (pnpcme) and to elucidate the identity of the bioactive compounds that comprise these larvicidal fractions. larvicidal assay was performed by subjecting 3rd to 4th a. aegypti instar larvae to pnpcme of p. nigrum. the pnpcme exhibite ...201525118563
optimization of reaction conditions to fabricate nano-silver using couroupita guianensis aubl. (leaf & fruit) and its enhanced larvicidal effect.currently bioactive principles of plants and their nanoproducts have been extensively studied in agriculture and medicine. in this study couroupita guianensis aubl. leaf and fruit extracts were selected for rapid and cost-effective synthesis of silver nanoparticles (leaf-lagnps and fruit-fagnps). various physiological conditions such as temperature, ph, concentration of metal ions, stoichiometric proportion of reaction mixture and reaction time showed influence on the size, dispersity and synthe ...201525062056
assessing dengue infection risk in the southern region of taiwan: implications for control.dengue, one of the most important mosquito-borne diseases, is a major international public health concern. this study aimed to assess potential dengue infection risk from aedes aegypti in kaohsiung and the implications for vector control. here we investigated the impact of dengue transmission on human infection risk using a well-established dengue-mosquito-human transmission dynamics model. a basic reproduction number (r 0)-based probabilistic risk model was also developed to estimate dengue inf ...201525007831
developing exon-primed intron-crossing (epic) markers for population genetic studies in three aedes disease vectors.aedes aegypti, aedes notoscriptus, and aedes albopictus are important vectors of many arboviruses implicated in human disease such as dengue fever. genetic markers applied across vector species can provide important information on population structure, gene flow, insecticide resistance, and taxonomy, however, robust microsatellite markers have proven difficult to develop in these species and mosquitoes generally. here we consider the utility and transferability of 15 ribosome protein (rp) exon-p ...201524895297
the single water-surface sweep estimation method accurately estimates very low (n = 4) to low-moderate (n = 25-100) and high (n > 100) aedes aegypti (diptera: culicidae) pupae numbers in large water containers up to 13 times faster than the exhaustive sweep and total count method and without any sediment contamination.to confirm that a single water-surface sweep-net collection coupled with three calibration factors (2.6, 3.0 and 3.5 for 1/3, 2/3 and 3/3 water levels, respectively) (wscf) could accurately estimate very low to high aedes aegypti pupae numbers in water containers more rapidly than the exhaustive 5-sweep and total count (estc) method recommended by who.201525418067
frequency of v1016i and f1534c mutations in the voltage-gated sodium channel gene in aedes aegypti in venezuela.the v1016i and f1534c mutations in the voltage-gated sodium channel gene have been associated with resistance to pyrethroids and ddt in aedes aegypti mosquitoes. a study was carried out to determine the frequency of i1016 and c1534 by real-time pcr in five natural populations of ae. aegypti in venezuela during 2008, 2010 and 2012, as well as in a strain selected with 0.14 µg of deltamethrin for 15 generations.201524935645
polyenylcyclopropane carboxylic esters with high insecticidal activity.pyrethroids are synthetic derivatives of naturally occurring pyrethrum. these molecules are widely used in agriculture for ant, fly and mosquito control and for lawn and garden care. pyrethroids are the optically active esters of 2,2-dimethyl-3-(2-methylpropenyl)-cyclopropane carboxylic acid, also known as chrysanthemic acid. however, their intense use has resulted in the development of resistance in many insect species. herein, specific structural modifications of the pyrethroid scaffold and th ...201524899413
screening for mosquito larvicidal activity of thai mushroom extracts with special reference to steccherinum sp against aedes aegypti (l.) (diptera: culicidae).for over 50 years, biological control of mosquito larvae has depended mainly on plant extracts, fish, bacteria, protozoa, filamentous fungi, viruses or nematodes. in this study, we screened 143 mushroom samples from 44 confirmed species in thailand for their mosquito larvicidal activity. one g% (w/v) aqueous extracts of dried powdered mushroom samples were tested against 3rd stage aedes aegypti larvae. four mushroom species, namely, thaeogyroporus porentosus, xylaria nigripes, chlorophyllum sp a ...201526867377
absence of aedes aegypti (l.) on an ecological island: competitive exclusion?ovitrap surveillance was conducted in 2012 and 2006 in malay and aboriginal villages on carey island. in each village, standard ovitraps were placed indoors and outdoors at randomly selected houses/locations. all l3 larvae recovered were identified up to species level. results demonstrated that only larvae of aedes albopictus were found in all the positive ovitraps placed indoors and outdoors. in 2012, a high ovitrap index (oi) of 66.7% indoor and 84.0% outdoor in the malay village; and 62.5% in ...201526863856
phenotypic and genotypic profile of pyrethroid resistance in populations of the mosquito aedes aegypti from goiânia, central west brazil.the mosquito aedes aegypti has evolved resistance to pyrethroid insecticides. the present study evaluated ae. aegypti from goiânia for the resistant phenotype and for mutations associated with resistance.201526516973
physical features and chitin content of eggs from the mosquito vectors aedes aegypti, anopheles aquasalis and culex quinquefasciatus: connection with distinct levels of resistance to desiccation.mosquito eggs are laid in water but freshly laid eggs are susceptible to dehydration, if their surroundings dry out at the first hours of development. during embryogenesis of different mosquito vectors the serosal cuticle, an extracellular matrix, is produced; it wraps the whole embryo and becomes part of the eggshell. this cuticle is an essential component of the egg resistance to desiccation (erd). however, erd is variable among species, sustaining egg viability for different periods of time. ...201526514070
new records of aedes aegypti at the southern limit of its distribution in buenos aires province, argentina. 201526611978
the effects of plant essential oils on escape response and mortality rate of aedes aegypti and anopheles minimus.the high throughput screening system (hitss) has been applied in insecticide behavioral response studies with various mosquito species. in general, chemical or natural compounds can produce a range of insect responses: contact irritancy, spatial repellency, knock-down, and toxicity. this study characterized these actions in essential oils derived from citronella, hairy basil, catnip, and vetiver in comparison to deet and picaridin against aedes aegypti and anopheles minimus mosquito populations. ...201526611967
intraspecific variation in desiccation survival time of aedes aegypti (l.) mosquito eggs of australian origin.aedes aegypti (l.) mosquitoes preferentially oviposit in natural and artificial receptacles where their eggs are able to withstand drying as water levels fluctuate. desiccation-resistant eggs also increase the potential for establishment in non-native habitats while providing logistical impediments to control programs. viability and mean survival times of eggs stored under three dryness conditions for up to 367 days were investigated among three field-derived colonies of australian ae. aegypti t ...201526611964
forced egg retention induced by diethyl-phenylacetamide diminishes the fecundity and longevity of dengue vectors.oviposition is an important event in the life cycle of mosquito which is a decisive factor for the fate of its progeny. if oviposition is prevented, the life cycle of mosquito is disrupted and the population growth can be reduced. diethyl-phenylacetamide (depa) is an effective topical repellent against various haematophagous insects. the study reports the egg retention effect and subsequent lethal effect of depa on the gravid females of aedes aegypti and ae. albopictus mosquitoes.201526714511
synthesis of silver nanoparticles using catharanthus roseus root extract and its larvicidal effects.phytosynthesis of silver nanoparticles has attracted considerable attention due to their biocompatibility, low toxicity, cost-effectiveness and being a novel method has an eco-friendly approach. biological activity of root extracts as well as synthesized silver nanoparticles of catharanthus roseus were evaluated against larvae of aedes aegyptiand culex quinquefasciatus. the structure and proportion of the synthesized nanoparticles was defined by exploitation ultraviolet spectrophotometry, x-ray ...201526688962
effect of isodillapiole on the expression of the insecticide resistance genes gste7 and cyp6n12 in aedes aegypti from central amazonia.the yellow fever mosquito aedes (stegomyia) aegypti is the main vector of dengue arbovirus and other arboviruses. dengue prevention measures for the control of a. aegypti involve mainly the use of synthetic insecticides. the constant use of insecticides has caused resistance in this mosquito. alternative studies on plant extracts and their products have been conducted with the aim of controlling the spread of the mosquito. dillapiole is a compound found in essential oils of the plant piper adunc ...201526681019
aedes aegypti eggs oviposited on water surface collected from field ovitraps in nova iguaçu city, brazil.aedes aegypti eggs can be collected from the water surface.201526676505
suitability of mixing fluorescent dye in adulticides and its impact on droplet characteristics and pesticide efficacy.fluorescent dyes are commonly used to help visualize insecticidal droplets or to trace movement of insecticides; however, the effect these dyes have on the insecticide's efficacy and droplet characteristics is unknown. this study evaluated the effects of mixing uvitex ob fluorescent dye with 5 adulticides on their efficacy in a wind tunnel. efficacy was determined via droplet size characteristics, spray flux (active ingredient [ai] deposition), and female adult aedes aegypti mortality. fyfanon® ...201526675457
interspecific cross-mating between aedes aegypti and aedes albopictus laboratory strains: implication of population density on mating behaviors.mating behavior between aedes aegypti and ae. albopictus, established colony strains were examined under laboratory conditions (30-cm(3) screened cages) for 5 consecutive days. the effect of selected male densities (30, 20, 10) and female density (20) on the number of swarming, mating pairs, eggs produced, and inseminated females were evaluated. male densities significantly increased swarming behavior, mating pairs, and egg production of heterospecific females, but female insemination was reduce ...201526675452
larvicidal activity, inhibition effect on development, histopathological alteration and morphological aberration induced by seaweed extracts in aedes aegypti (diptera: culicidae).to investigate the larvicidal activity, inhibition effect on development, histopathological alteration and morphological aberration induced by the extracts derived from seaweeds bryopsis pennata (b. pennata), sargassum binderi (s. binderi) and padina australis in aedes aegypti (ae. aegypti) larvae and to characterize the phytochemical components of the three seaweeds.201526706671
proteomic changes occurring in the malaria mosquitoes anopheles gambiae and anopheles stephensi during aging.the age of mosquitoes is a crucial determinant of their ability to transmit pathogens and their resistance to insecticides. we investigated changes to the abundance of proteins found in heads and thoraces of the malaria mosquitoes anopheles gambiae and anopheles stephensi as they aged. protein expression changes were assessed using two-dimensional difference gel electrophoresis and the identity of differentially expressed proteins was determined by using either matrix-assisted laser desorption i ...201526100052
characterization and bioassay for larvicidal activity of anacardium occidentale (cashew) shell waste fractions against dengue vector aedes aegypti.recent studies regarding the harmful effects of synthetic larvicides initiated the need to investigate for unconventional measures that are environmentally safe and target-specific against aedes aegypti larvae. thus, the main objectives of the study are to evaluate the larvicidal toxicity of the solvent fractions of anacardium occidentale shell wastes against the third and fourth instar larvae of a. aegypti and to compare the results with the commercial larvicide product. the shell wastes were e ...201526099240
surveillance of adult aedes mosquitoes in selangor, malaysia.to determine the effectiveness of using sticky traps and the ns1 dengue antigen kit for the surveillance of aedes mosquitoes for dengue control.201526094839
predation by asian bullfrog tadpoles, hoplobatrachus tigerinus, against the dengue vector, aedes aegypti, in an aquatic environment treated with mosquitocidal nanoparticles.aedes aegypti is a primary vector of dengue and chikungunya. the use of synthetic insecticides to control aedes populations often leads to high operational costs and adverse non-target effects. botanical extracts have been proposed for rapid extracellular synthesis of mosquitocidal nanoparticles, but their impact against predators of mosquito larvae has not been well studied. we propose a single-step method for the biosynthesis of silver nanoparticles (agnp) using the extract of artemisia vulgar ...201526091763
phytochemical profile and larvicidal properties of seed essential oil from nigella sativa l. (ranunculaceae), against aedes aegypti, anopheles stephensi, and culex quinquefasciatus (diptera: culicidae).the present study deals with investigation of larvicidal activity and their chemical constituents of the essential oil from the seeds of nigella sativa l. (ranunculaceae). totally, 18 chemical compounds were identified by gc and gc-ms analysis. thymol (19.13 %) and α-phellandrene (14.9 %) were identified as major chemical components followed by camphor (12.14 %), borneol (11.31 %), and carvacrol (8.65 %). the larval mortality was observed after 12 and 24 h of exposure period. the results reveale ...201526091760
thiosemicarbazones as aedes aegypti larvicidal.a set of aryl- and phenoxymethyl-(thio)semicarbazones were synthetized, characterized and biologically evaluated against the larvae of aedes aegypti (a. aegypti), the vector responsible for diseases like dengue and yellow fever. (q)sar studies were useful for predicting the activities of the compounds not included to create the qsar model as well as to predict the features of a new compound with improved activity. docking studies corroborated experimental evidence of aescp-2 as a potential targe ...201526087027
a sodium channel variant in aedes aegypti as a candidate pathogen sensor for viral-associated molecular patterns.recent work demonstrated that a splice variant of a human macrophage voltage-gated sodium channel expressed on endosomes acts as an intracellular sensor for dsrna, a viral-associated molecular pattern. here our goal was to identify a candidate gene in a clinically relevant invertebrate model with related cellular and pattern recognition properties. the para gene in drosophila and other insects encodes voltage-gated sodium channels with similar electrophysiological properties to those found in ve ...201526086103
localization and role of inward rectifier k(+) channels in malpighian tubules of the yellow fever mosquito aedes aegypti.malpighian tubules of adult female yellow fever mosquitoes aedes aegypti express three inward rectifier k(+) (kir) channel subunits: aekir1, aekir2b and aekir3. here we 1) elucidate the cellular and membrane localization of these three channels in the malpighian tubules, and 2) characterize the effects of small molecule inhibitors of aekir1 and aekir2b channels (vu compounds) on the transepithelial secretion of fluid and electrolytes and the electrophysiology of isolated malpighian tubules. usin ...201526079629
proteomic analysis of immunogenic proteins from salivary glands of aedes aegypti.humans develop anti-salivary proteins after arthropod bites or exposure to insect salivary proteins. this reaction indicates that vector bites have a positive effect on the host immune response, which can be used as epidemiological markers of exposure to the vector. our previous study identified two immunogenic proteins with molecular weights of 31 kda and 56 kda from salivary gland extract (sge) of aedes aegypti that cross-reacted with serum samples from dengue hemorrhagic fever (dhf) patients ...201526054892
the effect of photoperiod on life history and blood-feeding activity in aedes albopictus and aedes aegypti (diptera: culicidae).several studies have examined how climatic variables such as temperature and precipitation may affect life history traits in mosquitoes that are important to disease transmission. despite its importance as a seasonal cue in nature, studies investigating the influence of photoperiod on such traits are relatively few. this study aims to investigate how photoperiod alters life history traits, survival, and blood-feeding activity in aedes albopictus (skuse) and aedes aegypti (linnaeus). we performed ...201526047197
lethal ovitrap deployment for aedes aegypti control: potential implications for non-target organisms.in australia, dengue control combines source reduction with lethal ovitraps to reduce aedes aegypti populations during outbreaks. lethal ovitraps are considered a sustainable and environmentally friendly method of controlling container-inhabiting mosquitoes, however, to-date, this claim has not been quantified. this study assesses the potential impact of lethal ovitraps on non-target organisms when used to control ae. aegypti in tropical australia. for retention of specimens, we substituted stan ...201526047194
volatile semiochemical-conditioned attraction of the male yellow fever mosquito, aedes aegypti, to human hosts.we investigated the olfactory responses of male mosquitoes to kairomones of vertebrate hosts in a dual-port olfactometer. the behavioral responses of unmated and mated male and female mosquitoes from one to ten days old to human odors were compared to the odors of different human hosts. to evaluate the relationship between the age of male mosquitoes and their responses, we performed experiments with males at different ages. unmated ae. aegypti males, one to two days old, did not fly upwind to hu ...201526047178
effects of α,β-unsaturated lactones on larval survival and gut trypsin as well as oviposition response of aedes aegypti.lactones are organic cyclic esters that have been described as larvicides against aedes aegypti and as components of oviposition pheromone of culex quinquefasciatus. this work describes the effect of six α,β-unsaturated lactones (5a-5f) on survival of a. aegypti fourth instar larvae (l4). it is also reported the effects of the lactones on l4 gut trypsin activity and oviposition behavior of a. aegypti females. five lactones were able to kill l4 being the lactones 5a (lc50 of 39.05 ppm), 5e (lc50 ...201526044355
spatial models for prediction and early warning of aedes aegypti proliferation from data on climate change and variability in cuba.climate variability, the primary expression of climate change, is one of the most important environmental problems affecting human health, particularly vector-borne diseases. despite research efforts worldwide, there are few studies addressing the use of information on climate variability for prevention and early warning of vector-borne infectious diseases.201526027583
[omics of vector mosquitoes: a big data platform for vector biology and vector-borne diseases].recently the studies on mosquito genomics, transcriptomics and small rnaomics developed rapidly with the novel biotechnologies of the next generation sequencing techniques. the genome sequences of several important vector mosquitoes including anopheles gambiae, culex quinquefasciatus, and aedes aegypti have been published. the genome sizes vary among the different species of mosquitoes and are consistent with the number of the repeat regions. the released genome sequences facilitate gene cloning ...201526018253
annona muricata leaf extract-mediated silver nanoparticles synthesis and its larvicidal potential against dengue, malaria and filariasis vector.mosquitoes transmit several diseases which cause millions of deaths every year. the use of synthetic insecticides to control mosquitoes caused diverse effects to the environment, mammals, and high manufacturing cost. the present study was aimed to test the larvicidal activity of green synthesized silver nanoparticles using annona muricata plant leaf extract against third instar larvae of three medically important mosquitoes, i.e., aedes aegypti, anopheles stephensi, and culex quinquefasciatus. t ...201526002825
unique biochemical and molecular biological mechanism of synergistic actions of formamidine compounds on selected pyrethroid and neonicotinoid insecticides on the fourth instar larvae of aedes aegypti (diptera: culicidae).we recently reported that formamidine pesticides such as amitraz and chlordimeform effectively synergize toxic actions of certain pyrethroid and neonicotinoid insecticides in some insect species on the 4th instar larvae of aedes aegypti. here we studied the biochemical basis of the synergistic actions of the formamidines in amplifying the toxicity of neonicotinoids and pyrethroids such as dinotefuran and thiamethoxam, as well as deltamethrin-fenvalerate type of pyrethroids. we tested the hypothe ...201525987221
synergistic action of octopamine receptor agonists on the activity of selected novel insecticides for control of dengue vector aedes aegypti (diptera: culicidae) mosquito.studying insecticide resistance in mosquitoes has attracted the attention of many scientists to elucidate the pathways of resistance development and to design novel strategies in order to prevent or minimize the spread and evolution of resistance. here, we tested the synergistic action of piperonyl butoxide (pbo) and two octopamine receptor (or) agonists, amitraz (amz) and chlordimeform (cdm) on selected novel insecticides to increase their lethal action on the fourth instar larvae of aedes aegy ...201525987220
experience- and age-mediated oviposition behaviour in the yellow fever mosquito stegomyia aegypti (=aedes aegypti).in repeated behaviours such as those of feeding and reproduction, past experiences can inform future behaviour. by altering their behaviour in response to environmental stimuli, insects in highly variable landscapes can tailor their behaviour to their particular environment. in particular, female mosquitoes may benefit from plasticity in their choice of egg-laying site as these sites are often temporally variable and clustered. the opportunity to adapt egg-laying behaviour to past experience als ...201525982411
larvicidal activity against aedes aegypti of foeniculum vulgare essential oils from portugal and cape verde.dengue is a potentially fatal mosquito-borne infection with 50 million cases per year and 2.5 billion people vulnerable to the disease. this major public health problem has recurrent epidemics in latin america and occurred recently in cape verde and madeira island. the lack of anti-viral treatment or vaccine makes the control of mosquito vectors a high option to prevent virus transmission. essential oil (eo) constituents can affect insect's behaviour, being potentially effective in pest control. ...201525973508
imidacloprid impairs the post-embryonic development of the midgut in the yellow fever mosquito stegomyia aegypti (=aedes aegypti).the mosquito stegomyia aegypti (=aedes aegypti) (diptera: culicidae) is a vector for the dengue and yellow fever viruses. as blood digestion occurs in the midgut, this organ constitutes the route of entry of many pathogens. the effects of the insecticide imidacloprid on the survival of st. aegypti were investigated and the sub-lethal effects of the insecticide on midgut development were determined. third instar larvae were exposed to different concentrations of imidacloprid (0.15, 1.5, 3.0, 6.0 ...201525968596
oxime derivatives with larvicidal activity against aedes aegypti l.oximes containing secondary metabolites constitute an important group of bioactive compounds and have been described and frequently updated in the literature due to their pharmacological properties. thus, the aim of this study was to evaluate the larvicidal activity of a series of fourteen structurally related [1,4]-benzoquinone mono-oximes on third-instar aedes aegypti larvae and to investigate structure-activity relationships (sar) of these compounds. results of larvicidal assay revealed that ...201525956398
involvement of metabolic resistance and f1534c kdr mutation in the pyrethroid resistance mechanisms of aedes aegypti in india.pesticide resistance poses a serious problem for worldwide mosquito control programs. resistance to insecticides can be caused by an increased metabolic detoxification of the insecticide and/or by target site insensitivity. in the present study, we estimated the tolerance of indian aedes aegypti populations using adult bioassays that revealed high resistance levels of the field populations to permethrin (rr-6, 5.8 and 5.1 folds) compared to our susceptible population. enzymatic assays revealed i ...201525944353
susceptibility to temephos, permethrin and deltamethrin of aedes aegypti (diptera: culicidae) from muang district, phitsanulok province, thailand.to investigate the susceptibility to temephos, permethrin and deltamethrin of aedes aegypti (ae. aegypti), collected from areas with high incidence of dengue hemorrhagic fever cases in phitsanulok province, thailand.201525901918
frizzled 2 is a key component in the regulation of tor signaling-mediated egg production in the mosquito aedes aegypti.the wnt signaling pathway was first discovered as a key event in embryonic development and cell polarity in drosophila. recently, several reports have shown that wnt stimulates translation and cell growth by activating the mtor pathway in mammals. previous studies have demonstrated that the target of rapamycin (tor) pathway plays an important role in mosquito vitellogenesis. however, the interactions between these two pathways are poorly understood in the mosquito. in this study, we hypothesized ...201525890109
structure-activity relationship (sar) modelling of mosquito larvicides.an attempt was made to derive structure-activity models allowing the prediction of the larvicidal activity of structurally diverse chemicals against mosquitoes. a database of 188 chemicals with their activity on aedes aegypti larvae was constituted from analysis of original publications. the activity values were expressed in log 1/ic50 (concentration required to produce 50% inhibition of larval development, mmol). all the chemicals were encoded by means of codessa and autocorrelation descriptors ...201525864415
territorial analysis of aedes aegypti distribution in two colombian cities: a chorematic and ecosystem approach.a territorial analysis of aedes aegypti density was conducted in two colombian cities using an ecosystem and chorematic approach. entomological and behavioral data (by cluster) and information on the urban context were used to analyze the relationship between territorial structures and dynamics and vector density. the results were represented in graphic (chorematic) models. arauca showed higher vector density than armenia. higher density was related to unplanned urbanization, flood-prone areas, ...201525859719
gc-ms analysis of bioactive components and synthesis of gold nanoparticle using chloroxylon swietenia dc leaf extract and its larvicidal activity.a rapid bio-reduction of chloroauric acid (haucl4) was achieved by chloroxylon swietenia dc leaf extract (csle), which resulted in the formation of well dispersed c. swietenia gold nanoparticles (csgnps). the formation of gnps was confirmed by color changes from yellowish green to purple and their characteristic peak at 545 nm. the characterization of synthesized csgnps was made through x-ray diffraction (xrd), fourier transform infrared spectroscopy (ftir), high resolution transmission electron ...201525854160
evaluation of the atraedes™ lure for collection of culex quinquefasciatus in gravid traps.the typical attractant used in gravid trapping of culex quinquefasciatus is an aged infusion of organic materials, which can change in attractiveness over time. a standardized chemical attractant dispenser derived from grass infusion, the atraedes™ lure, has been produced for the surveillance of the dengue vector aedes aegypti. a study using this lure in combination with the centers for disease control and prevention gravid traps was conducted in tanga, tanzania. the addition of the lure to trap ...201525843185
evaluation of a new spraying machine for barrier treatment and penetration of bifenthrin on vegetation against mosquitoes.the effectiveness and penetration of a novel, truck-mounted mist sprayer (3wc-30-4p provided by american longray) was evaluated with bifenthrin in a large, park-like setting with historic floodwater and woodland mosquito populations. efficacy evaluations were determined through adult population collections and excised leaf bioassays. trapping results showed a mean reduction of 77% in mosquito populations in the treated area for 5 sampling events up to 4 wk posttreatment. leaf bioassays revealed ...201525843180
larvicidal activity of naturally occurring naphthalenedione and its structurally related analogs against three mosquito species.the objective of this study was to determine the larvicidal activity of an active compound isolated from cercis chinensis heartwood and its structurally related analogs against 4th-stage aedes aegypti, culex pipiens pallens, and ae. togoi. the larvicidal compound of c. chinensis was isolated with the use of various chromatographic techniques and identified as analogs of 1,4-naphthalenedione. based on the median lethal concentration (lc(50)) values of commercially procured analogs against ae. aeg ...201525843178
laboratory studies of aedes aegypti attraction to ketones, sulfides, and primary chloroalkanes tested alone and in combination with l-lactic acid.the attraction of female aedes aegypti to single compounds and binary compositions containing l-lactic acid and an additional saturated compound from a set of ketones, sulfides, and chloroalkanes was studied using a triple-cage dual-port olfactometer. these chemical classes were studied because of their structural relation to acetone, dimethyl disulfide, and dichloromethane, which have all been reported to synergize attraction to l-lactic acid. human odors, carbon dioxide, and the binary mixture ...201525843177
two novel bioassays to assess the effects of pyrethroid-treated netting on knockdown-susceptible versus resistant strains of aedes aegypti.we describe 2 new mosquito bioassays for use with insecticide-treated netting or other textiles. the 1st is a cylinder bioassay in which a mosquito is forced to contact treated material regardless of where it lands within the bioassay construct. the 2nd is a repellency/irritancy and biting-inhibition bioassay (ribb) in which human arms and breath are used as attractants. mosquitoes have the choice to pass through holes cut in untreated or treated netting to move from a center release chamber int ...201525843176
comparison of bg-sentinel® trap and oviposition cups for aedes aegypti and aedes albopictus surveillance in jacksonville, florida, usa.the bg-sentinel® (bgs) trap and oviposition cups (ocs) have both proven effective in the surveillance of aedes species. this study aimed to determine which of the 2 traps could best characterize the relative population sizes of aedes albopictus and aedes aegypti in an urban section of jacksonville, fl. until 1986, ae. aegypti was considered the dominant container-breeding species in urban northeastern florida. since the introduction of ae. albopictus, ae. aegypti has become almost completely ext ...201525843173
studies on insecticide susceptibility of aedes aegypti (linn) and aedes albopictus (skuse) vectors of dengue and chikungunya in andaman and nicobar islands, india.dengue and chikungunya are important arboviral infections in the andaman islands. competent vectors viz. aedes aegypti and aedes albopictus are widely prevalent. the most effective proven method for interrupting the transmission of these arboviruses is vector control, mediated through insecticides. currently, ddt and temephos are the insecticides used for vector control in these islands. lack of information on susceptibility necessitated assessing the susceptibility profile of a. aegypti and a. ...201526344869
identification and characterization of novel membrane-bound prl protein tyrosine phosphatases from setaria cervi, a bovine filarial parasite.a significant amount of protein tyrosine phosphatase (ptp) activity was detected in the detergent-soluble membrane-bound fraction of setaria cervi, a bovine filarial parasite. the membrane-bound ptp activity was significantly inhibited when the adult parasites were exposed to compounds having antifilarial activity like aspirin and sk7 as well as phenylarsine oxide, a specific ptp inhibitor suggesting that this activity is stress regulated. further, this enzyme was purified as a single protein of ...201526341797
efficacy of essential oil from cananga odorata (lamk.) hook.f. & thomson (annonaceae) against three mosquito species aedes aegypti (l.), anopheles dirus (peyton and harrison), and culex quinquefasciatus (say).the essential oil of cananga odorata flowers was evaluated for oviposition-deterrent, ovicidal, insecticidal, and repellent activities toward three mosquito species: aedes aegypti, anopheles dirus, and culex quinquefasciatus. oviposition deterrence of the oil was evaluated on gravid females using oviposition deterrence bioassay. the results showed that 10 % ca. odorata exhibited high percent effective repellency against oviposition at 99.4 % to ae. aegypti, 97.1 % to an. dirus, and 100 % to cx. ...201526337270
evaluation of insect growth regulators against field-collected aedes aegypti and aedes albopictus (diptera: culicidae) from malaysia.susceptibility status of aedes aegypti (l.) and aedes albopictus skuse larvae obtained from 12 states in malaysia were evaluated against five insect growth regulators (igrs), namely, pyriproxyfen, methoprene, diflubenzuron, cyromazine, and novaluron under laboratory conditions. field populations of ae. aegypti exhibited moderate resistance toward methoprene and low resistance toward pyriproxyfen, with resistance ratios of 12.7 and 1.4, respectively, but susceptibility to diflubenzuron, cyromazin ...201526336304
interspecific larval competition differentially impacts adult survival in dengue vectors.mosquitoes often experience intraspecific and interspecific competition among larvae attributable to high densities and nutrient limitation, especially container mosquitoes including aedes aegypti (l.) and aedes albopictus (skuse). density-dependent effects on larvae impact adult production and adult traits that influence transmission of arboviruses. to improve our understanding of the mechanisms by which density-dependence influences transmission and identify species-specific traits, we tested ...201526336301
essential oils of echinophora lamondiana (apiales: umbelliferae): a relationship between chemical profile and biting deterrence and larvicidal activity against mosquitoes (diptera: culicidae).the essential oils from the flower, leaf, and stem of echinophora lamondiana b.yildiz et z.bahcecioglu were analyzed by gas chromatography-flame ionization detection and gas chromatography-mass spectrometry. in total, 41, 37, and 44 compounds were identified, which accounted for 98.0, 99.1, and 97.0% of flower, leaf, and stem essential oils, respectively. the monoterpenic hydrocarbons were found to be high in all samples of the essential oils. the major components of essential oils from flower, ...201526336285
late-instar behavior of aedes aegypti (diptera: culicidae) larvae in different thermal and nutritive environments.the effects of temperature on ectotherm growth have been well documented. how temperature affects foraging behavior is less well explored, and has not been studied in larval mosquitoes. we hypothesized that temperature changes foraging behavior in the aquatic larval phase of the mosquito, aedes aegypti l. based on empirical results in other systems, we predicted that foraging effort would increase at higher temperatures in these insects. we tested this prediction over three temperature condition ...201526336228
larvicidal and biting deterrent activity of essential oils of curcuma longa, ar-turmerone, and curcuminoids against aedes aegypti and anopheles quadrimaculatus (culicidae: diptera).essential oils and extract of curcuma longa, ar-turmerone, and curcuminoids were evaluated for their larvicidal and deterrent activity against mosquitoes. ar-turmerone and curcuminoids constituted 36.9, 24.9 and 50.6% of rhizome oil, leaf oil, and rhizome extract, respectively. ar-turmerone was the major compound of the rhizome oil (36.9%) and leaf oil (24.9%). the ethanolic extract had 15.4% ar-turmerone with 6.6% bisdesmethoxycurcumin, 6.1% desmethoxycurcumin, and 22.6% curcumin. in in vitro s ...201526336212
potential effects of climate change on ecological interaction outcomes between two disease-vector mosquitoes: a mesocosm experimental study.the objective of this study was to experimentally assess the effects of different climate change scenarios on the outcomes of interactions between aedes aegypti (l.) and culex quinquefasciatus (say) (diptera: culicidae) larvae. the experimental design maintained a constant density of specimens while the proportion of the species in different experimental climate change scenarios varied. our results indicate that survival of the two species was not affected, but larval development and pupation ti ...201526336208
aedes aegypti (diptera: culicidae) in mauritania: first report on the presence of the arbovirus mosquito vector in nouakchott.aedes aegypti l. (diptera: culicidae) is a major vector of yellow fever, dengue, and chikungunya viruses throughout tropical and subtropical areas of the world. although the southernmost part of mauritania along the senegal river has long been recognized at risk of yellow fever transmission, aedes spp. mosquitoes had never been reported northwards in mauritania. here, we report the first observation of aedes aegypti aegypti (l.) and aedes (ochlerotatus) caspius (pallas, 1771) in the capital city ...201526335483
real-time pcr tests in dutch exotic mosquito surveys; implementation of aedes aegypti and aedes albopictus identification tests, and the development of tests for the identification of aedes atropalpus and aedes japonicus japonicus (diptera: culicidae).since 2009, the netherlands food and consumer product safety authority carries out surveys focusing on, amongst others, the presence of invasive mosquito species (ims). special attention is given to exotic container-breeding aedes species aedes aegypti (l.), aedes albopictus (skuse), aedes atropalpus (coquillett), and aedes japonicus japonicus (theobald). this study describes the implementation of real-time pcr tests described by hill et al. (2008) for the identification of ae. aegypti and ae. a ...201526334807
comparison of field and laboratory-based tests for behavioral response of aedes aegypti (diptera: culicidae) to repellents.the repellent and irritant effects of three essential oils-clove, hairy basil, and sweet basil-were compared using an excito-repellency test system against an insecticide-resistant strain of aedes aegypti (l.) females from pu teuy, kanchanaburi province. deet was used as the comparison standard compound. tests were conducted under field and controlled laboratory conditions. the most marked repellent effect (spatial noncontact assay) among the three test essential oils was exhibited by sweet basi ...201526470388
the use of a chemically defined artificial diet as a tool to study aedes aegypti physiology.aedes aegypti mosquitoes obtain from vertebrate blood nutrients that are essential to oogenesis, such as proteins and lipids. as with all insects, mosquitoes do not synthesize cholesterol but take it from the diet. here, we used a chemically defined artificial diet, hereafter referred to as substitute blood meal (sbm), that was supplemented with cholesterol to test the nutritional role of cholesterol. sbm-fed and blood-fed mosquitoes were compared regarding several aspects of the insect physiolo ...201526578294
trace samples of human blood in mosquitoes as a forensic investigation tool.investigations of any type of crime invariably starts at the crime scene by collecting evidence. thus, the purpose of this research was to collect and analyze an entomological trace from an environment that is similar to those of indoor crime scenes. hematophagous mosquitoes were collected from two residential units; saliva of volunteers that were residents in the units was also collected for genetic analysis as reference samples. we examined the allele frequencies of 15 short tandem repeat loci ...201526600546
a simple and affordable membrane-feeding method for aedes aegpyti and anopheles minimus (diptera: culicidae).this study developed an artificial feeding (af) method to replace direct host feeding (dhf) for the maintenance of aedes aegypti and anopheles minimus mosquito colonies. the procedure can be adopted by all laboratories due to its simple and affordable materials and design. the apparatus consists of heparinized cow blood contained in a 5cm diameter glass petri dish with 5cm(2) parafilm m (bemis(®)) stretched thinly over the top, with a pre-heated bag of vegetable oil placed underneath to keep the ...201526440474
control of aedes aegypti and ae. albopictus, the vectors of dengue and chikungunya, by using pheromone c21 with an insect growth regulator: results of multicentric trials from 2007-12 in india.aedes mosquito control has gained much importance nowadays in view of rise in number of reported cases of dengue and chikungunya in india and other countries. in the present study, c21 attracticide (containing a pheromone and an insect growth regulator—igr, developed by defence research and development establishment (drde), gwalior, india was tested for its feasibility for surveillance and control of aedes mosquito in a multicentric mode from october 2007 to june 2012 in urban (delhi, and bengal ...201526418653
toxicity of cephalaria species and their individual constituents against aedes aegypti.crude acetone and ethanol extracts of the aerial parts of 21 cephalaria species collected from turkey were investigated for larvicidal and adult topical activity against aedes aegypti. the ethanol extracts from c. elazigensis var. purpurea, c. anatolica, and c. elmaliensis possessed the highest mortality against first instar ae. aegypti larvae. luteolin-7-o-β-d-glycoside (1), isolated from c. elmaliensis ethanol extract, demonstrated 33% and 53% mortality at 0.1 μg/ml concentration against first ...201526411009
spectral and hrtem analyses of annona muricata leaf extract mediated silver nanoparticles and its larvicidal efficacy against three mosquito vectors anopheles stephensi, culex quinquefasciatus, and aedes aegypti.mosquitoes transmit various diseases which mainly affect the human beings and every year cause millions of deaths globally. currently available chemical and synthetic mosquitocidal agents pose severe side effects, pollute the environment vigorously, and become resistance. there is an urgent need to identify and develop the cost effective, compatible and eco-friendly product for mosquito control. the present study was aimed to find out the larvicidal potential of aqueous crude extract and green s ...201526410042
multi-cue integration: how female mosquitoes locate a human host.to reproduce, the female yellow fever mosquito has to find a human host. there are many potential cues available to guide such navigation: exhaled carbon dioxide, a plethora of skin odors, the host's visual and heat signatures and, close by, moisture. recent work is shedding new light on how these are integrated by the mosquito in targeting a human host.201526394099
modification of the suna trap for improved survival and quality of mosquitoes in support of epidemiological studies.monitoring adult mosquito populations provides information that is critical for assessing risk of vector-borne disease transmission. the recently developed suna trap was found to be a very effective trap when baited with an attractive odor blend. a modification of this trap was tested to improve its function as a tool for monitoring mosquito populations, including anopheles coluzzii (an. gambiae sensu stricto molecular form m), aedes aegypti, and culex pipiens. the modified suna trap (suna-m) wa ...201526375903
schools as potential risk sites for vector-borne disease transmission: mosquito vectors in rural schools in two municipalities in colombia.dengue and other vector-borne diseases are of great public health importance in colombia. vector surveillance and control activities are often focused at the household level. little is known about the importance of nonhousehold sites, including schools, in maintaining vector-borne disease transmission. the objectives of this paper were to determine the mosquito species composition in rural schools in 2 municipalities in colombia and to assess the potential risk of vector-borne disease transmissi ...201526375902
a multiobjective optimization approach for combating aedes aegypti using chemical and biological alternated step-size control.dengue epidemics, one of the most important viral disease worldwide, can be prevented by combating the transmission vector aedes aegypti. in support of this aim, this article proposes to analyze the dengue vector control problem in a multiobjective optimization approach, in which the intention is to minimize both social and economic costs, using a dynamic mathematical model representing the mosquitoes' population. it consists in finding optimal alternated step-size control policies combining che ...201526362231
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