Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| heterologous immunity in rodent malaria: comparison of the degree of cross-immunity generated by vaccination with that produced by exposure to live infection. | the degree of heterologous immunity occurring between the different rodent malarias was examined. mice immunized with a killed vaccine consisting of formalin-fixed blood-stage parasites mixed with saponin, were challenged with homologous or heterologous parasites and the degree of protection assessed by monitoring the number of survivors and the duration and severity of parasitaemia. the results indicated that vaccination conferred strong cross-protection between two different plasmodium yoelii ... | 1983 | 6357121 |
| failure to induce splenomegaly in balb/c mice using rodent malaria culture supernatants. | uninfected male and female balb/c mice were given a twice weekly intraperitoneal injection, of supernatants obtained from 24-h cultures of plasmodium berghei-infected and control mouse red blood cells, for 5 weeks. the mice were then weighed along with uninjected controls. all the animals were sacrificed by chloroform anaesthesia and their spleen weights measured. mice receiving malaria culture supernatants had statistically similar spleen weights to those receiving control culture supernatants. ... | 1983 | 6358008 |
| malaria-induced lymphokines: stimulation of macrophages for enhanced phagocytosis. | culture supernatants from antigen-pulsed spleen cells of mice infected previously with either bcg or plasmodium chabaudi were used to study macrophage activation as judged by phagocytosis of immunoglobulin g-sensitized erythrocytes and plasmodium berghei- and p. chabaudi-infected erythrocytes. resident peritoneal macrophages were incubated in vitro with spleen cell factor and then assayed for ingestion of immunoglobulin g-sensitized or parasitized erythrocytes. macrophages activated with bcg-ind ... | 1983 | 6358030 |
| [effect of a milk diet on parasitemia suppression and on the development of humoral immunity during the course of malarial infection in mice]. | 1983 | 6358774 | |
| synthesis and biological activity of some di(nitrothienyl)- and di(acetylaminothienyl)sulfones. | some new di(nitrothienyl)- and di(acetylaminothienyl)sulfones were synthesized. compounds (i)-(vi) were active against several gram-positive bacteria, in vitro. di(5-acetylamino-2-thienyl)sulfone (vii) showed a mild antimalarial activity against a drug-sensitive strain of p. berghei in mice. | 1983 | 6360709 |
| [synthesis, toxicity and antimalarial effects of bispyroquine]. | 1983 | 6223506 | |
| plasmodium berghei: a mouse model for the "sudden death" and "malarial lung" syndromes. | a mouse model for the "sudden death" and "malarial lung" syndromes is described. mice of the c3h/z strain succumb suddenly approximately 7 days after an infection with plasmodium berghei becomes patent, at a time when parasitemia is still moderate (6 to 8%). death could be shown to be due to anaphylactoid shock, probably induced by soluble immune complexes. increased vascular permeability caused transudation and leakage of serum proteins into the interstitium and the alveoli. the lungs were foun ... | 1983 | 6223835 |
| prevention of cerebral malaria by adoptive transfer of malaria-specific cultured t cells into mice infected with plasmodium berghei. | murine t cell populations specific for plasmodium berghei parasites were generated in vitro from balb/c immune lymph node cells. the malaria-specific t lymphocytes were shown: a) to proliferate specifically in vitro in response to stimulation with p. berghei-infected red blood cells; b) to exhibit the thy-1+, lyt-1+2- cell surface phenotype; c) to provide specific helper activity for an in vitro anti-hapten (tnp) plaque-forming cell antibody response; and d) to protect p. berghei-infected mice f ... | 1983 | 6224859 |
| anti-lymphocyte antibodies in lethal mouse malaria. ii. induction of an autoantibody specific suppressor t cell by non-lethal p. yoelii. | the anti-lymphocyte autoantibody response to irradiated lethal plasmodium berghei malaria parasites in normal mice was significantly reduced when recipients were pre-treated with splenic t cells from mice recovered from a non-lethal plasmodium yoelii infection. suppression was specific for the autoantibody and did not affect the antibody response to the parasite. experiments involving sequential p. yoelii-p. berghei infections in situ revealed that recovery from p. berghei was possible when the ... | 1983 | 6225580 |
| [7 antimalarials in the treatment of mice experimentally infected with pyronaridine-resistant plasmodium berghei]. | 1983 | 6230874 | |
| ultrastructural studies of a vesicle system associated with endoplasmic reticulum in exo-erythrocytic forms of plasmodium berghei. | fine structural studies of a specialized vesicle system associated with the endoplasmic reticulum (er) of exo-erythrocytic plasmodium berghei suggest that this system may be the equivalent of a golgi apparatus. patches of er, randomly distributed in the cytoplasm of developing parasites, are formed of smooth and ribosome-studded cisternae intermingled with each other. the vesicle systems are located between as well as at the edges of er aggregates and appear to be in different stages of budding ... | 1983 | 6191028 |
| anti-red blood cell autoantibodies induced in rat by plasmodium berghei infection bind to a cross-reacting determinant present also in other cell types. | it has been previously shown that rats injected with red blood cells (rbc) infected with plasmodium berghei make autoantibodies reacting with normal rat rbc as demonstrated by a staphylococcal protein a binding assay. in this study, the serum of the infected rats was found to contain also antibodies to several other types of rat cells (lymphoid, liver, and kidney cells and brain tissue) as well as against rbc and lymphoid cells of mice and sheep, but not of man. rat, mouse, and sheep rbc and rat ... | 1983 | 6191899 |
| [oxidative damage of mice erythrocytes infected with plasmodium berghei]. | by using the model of infection with plasmodium berghei in white mice the attempt was made to explain the oxidative damages of red blood cells as a cause for haemolysis. in addition to a diminished new formation of erythrocytes there was an increased cell lysis under the impact of infection. without any changes of the met-hb-percentage and of heinz bodies an increase of gsh and gssg could be measured. the conclusion was drawn that a damage of red blood cells caused by oxidation may occur by chan ... | 1983 | 6196273 |
| [effect of formate on mouse erythrocytes in vivo and in vitro]. | the effect of formiat was tested in erythrocytes of mice damaged by oxidisation. decrease of heinz's bodies, stabilisation of reduced glutathion, and increase of enzyme activities by referring to an example of superoxidismutase could be identified as a positive effect. possibilities of formiat reactions in the metabolism of red blood cells are discussed. | 1983 | 6200395 |
| preparation, properties and biological activity of natural and semisynthetic urethanes of monensin. | conversion of the monovalent polyether antibiotic monensin into a series of urethane derivatives substituted at c-26 causes a ten-fold increase in the cation transporting properties of the antibiotic as well as making the resulting semisynthetic urethanes divalent ionophores. these changes must in part account for the enhanced antimicrobial activities of the urethanes. the most active derivatives are the phenylurethanes which are ten times more active in vitro against gram-positive bacteria and ... | 1983 | 6630078 |
| a plasmodium protein kinase that is developmentally regulated, stimulated by spermine, and inhibited by quercetin. | plasmodium berghei-infected murine red cells possess protein kinase activity that is associated with the isolated parasites. schizonts contain significantly higher levels of this protein kinase than the more immature forms, suggesting a relationship between this enzyme activity and parasite development. partially purified protein kinase has a km for atp of approximately 30 microms, whereas the km for gtp is approximately 300 microms and the substrate preference is phosvitin greater than casein m ... | 1983 | 6654993 |
| killing of blood-stage murine malaria parasites by hydrogen peroxide. | both nonlethal plasmodium yoelii and lethal plasmodium berghei were killed in vitro by hydrogen peroxide at concentrations as low as 10(-5) m. higher concentrations were required in the presence of added normal erythrocytes. injection of hydrogen peroxide in vivo significantly reduced p. yoelii parasitemia but had less effect on p. berghei. | 1983 | 6822428 |
| plasmodium berghei: acid-insensitive phosphofructokinase in infected mouse erythrocytes. | 1982 | 6459948 | |
| [interaction of chloroquine with ferriprotophorphyrin ix. nuclear magnetic resonance study]. | chloroquine is still the antimalarial drug which is the most utilized. nevertheless the molecular mode of action of this drug is not very well understood. when mouse erythrocytes injected with plasmodium berghei are exposed to chloroquine, the first biochemical event is rapid accumulation of the drug. this process is energy dependent, saturable and competitively inhibited by drugs of the same therapeutic class (quinine, amodiaquine, mefloquine). receptors for chloroquine have been proposed for t ... | 1982 | 6818999 |
| inhibitory action of alpha-difluoromethylornithine on rodent malaria (plasmodium berghei). | 1982 | 6819662 | |
| a malaria 'mitogen'-induced depressed immune response to meningococcal polysaccharide vaccine in balb/c mice. | uninfected female balb/c mice were given a 4-daily intraperitoneal injection, of supernatants obtained from 24-h cultures of plasmodium berghei infected and control mouse red blood cells, for 20 days. each mouse was subsequently injected subcutaneously with 10 mg meningococcal (groups a and c combined) polysaccharide vaccine. mean meningococcal haemagglutinating antibody titres obtained in mice pretreated with control supernatants were consistently higher, than those obtained in mice pretreated ... | 1982 | 6819992 |
| the antimalarial activity of n-benzyl-oxydihydrotriazines. iii. the activity of 4,6-diamino-1,2-dihydro-2,2-dimethyl-1-(2,4,5,-trichloropropyloxy)-1,3,5-triazine hydrobromide (brl 51084) and hydrochloride (brl 6231). | 1982 | 7044322 | |
| the antimalarial activity of n-benzyl-oxydihydrotriazines. iv. the development of resistance to brl 6231 (4,6-diamino-1,2-dihyydro-2-,2-dimethyl-1-(2,4,5-trichloropropyloxy)-1,3,5 triazine hydrochloride) by plasmodium berghei. | 1982 | 7044325 | |
| [inflammation and resistance of mice against "plasmodium berghei" (author's transl)]. | an inflammatory reaction induced in mice by a subcutaneous injection of magnesium silicate embedded in a calcium phosphate gel is followed by an increased resistance against plasmodium berghei. the occurrence of this increased resistance against plasmodium berghei. the occurrence of this increased resistance is related to the time elapsed between the induction of the inflammatory process and the infection. the delayed mortality is correlated with a slowed development of parasitemia. it is hypoth ... | 1982 | 7046621 |
| cross-reactivity and cross-protection in murine malaria. | 1982 | 7046657 | |
| the course of plasmodium berghei infection in mice latently infected with toxoplasma gondii. | the course of infection with 2 different virulent strains of plasmodium berghei was investigated in mice latently infected with toxoplasma gondii. when given the highly virulent anka strain of p. berghei all toxoplasma-infected mice died but the survival time was prolonged. after infection with the less virulent strain k 173 mice could survive the subsequent infection. in these cases levels of parasitemia depended upon the duration of the t. gondii infection. mice infected for about 6 weeks with ... | 1982 | 7047202 |
| heterogeneity in filterability of erythrocytes from malaria (plasmodium berghei)-infected blood. | erythrocytes from plasmodium berghei-infected blood show a decrease in deformability with increasing parasitaemia, as measured by filterability through polycarbonate sieves. a major fraction of cells carrying mature parasites and a smaller fraction carrying ring-stage parasites account for the obstruction of filtration, while the remaining infected cells do not contribute to the decrease in filterability. the relation of filterability to metabolic status in infected cells is discussed. | 1982 | 7047205 |
| adoptive transfer of resistance to plasmodium berghei with spleen cells and serum from fansidar-cured mice. | the ability of splenic leukocytes or serum to transfer immunity to plasmodium berghei was studied in c57bl/6 mice. splenic leukocytes or serum was removed from mice which had been inoculated previously 1 to 4 or 5 times with p. berghei and cured 1 to 4 or 5 times with fansidar (pyrimethamine plus sulfadoxine) and transferred to syngeneic recipients 1 or 2 days before parasite challenge. partial protection was observed in recipients of immune serum or unfractionated splenic leukocytes. significan ... | 1982 | 7047391 |
| enhanced resistance to plasmodium berghei in mice previously infected with trichinella spiralis. | infection with trichinella spiralis larvae greatly enhanced the resistance of adult mice against fatal infection with plasmodium berghei given 10 and 30 days after t. spiralis infection. mice infected with t. spiralis had a markedly activated mononuclear phagocytic system and significantly low reticulocyte levels at the time the mice were challenged with p. berghei. therefore, the partially subdued parasitaemia and prolonged survival of trichinella-plasmodium-infected mice may be attributed, in ... | 1982 | 7048209 |
| asynchronous maturation of plasmodium berghei exo-erythrocytic forms in rats. | sporozoites of plasmodium berghei berghei (nk-65 strain) were inoculated intravenously into four-week-old cfn rats. liver biopsies were taken at intervals and the density of exo-erythrocytic forms per mm3 of liver were estimated. results demonstrated asynchronous maturation and the gradual disappearance of exo-erythrocytic forms during the period 48 to 72 hours after infection. | 1982 | 7048651 |
| mosquito infectivity is directly related to the proportion of repetitive dna in plasmodium berghei. | a strain of plasmodium berghei (nk 65) was followed during syringe transmission in mice for over 120 passages after the last complete cycle, while the following parameters were monitored: (a) capacity to infect mosquitoes, inducing oocyst formation; (b) presence in the peripheral blood of morphologically identifiable gametocytes; (c) presence of a repetitive component in the dna extracted from intraerythrocytic population. the suggestion of a possible role of this component in gametogenesis came ... | 1982 | 7050700 |
| resistance to superinfection with plasmodium berghei in rats fed a protein-free diet. | the development of resistance to reinfection with plasmodium berghei was studied in rats in which the primary infection had been almost totally suppressed by feeding a protein-free diet (peak parasitaemia 0.5%; patent for only the first four days after inoculation) on days 5, 9, 15, 23 and 28 after primary inoculation groups of animals were challenged with the same strain of parasite. at the same time the diet was changed to that of a 17% casein formula. the development of resistance as judged b ... | 1982 | 7051455 |
| cerebral malaria in inbred mice. i. a new model and its pathology. | plasmodium berghei anka was tested for its usefulness as a model for cerebral malaria in inbred mouse strains a, a2g, a/j, c57l, sjl/j and swr. a suitable model was obtained in a or a/j strain mice. mortality was 100% after five to eight days with brain haemorrhages occurring terminally. the histopathology is described. the model was stable after six blood passages at 5- to 7-day intervals. chloroquine abolished the haemorrhages and no intercurrent viral or blood protozoal infections were detect ... | 1982 | 7051459 |
| culture of the exoerythrocytic liver stages of plasmodium berghei sporozoites in rat hepatocytes. | 1982 | 7051460 | |
| metabolism of babesia parasites in vitro. amino acid production by babesia rodhaini compared to plasmodium berghei. | the in vitro amino acid production by babesia rodhaini, plasmodium berghei and uninfected rat erythrocytes was determined following 4 and 18 h of incubation in krebs ringer medium. both parasites produced excess free amino acid. b. rodhaini produced up to 150 times and p. berghei up to 1,100 times as much free amino acid as parasite-free rat erythrocytes. the composition of excess amino acids produced by both parasites had a statistically significant concordance with the amino acid composition o ... | 1982 | 7052039 |
| surface membrane proteins and glycoproteins of red blood cells from normal and anaemic mice. | 1. the surface membrane proteins of red blood cells from normal, hyperbled or acetylphenylhydrazine-treated balb/c mice and nzb mice of different ages were labelled by lactoperoxidase-catalyzed radioiodination. sialoglyoproteins were labelled by periodate/nab3h4 or galactose oxidase +/- neuraminidase/na3h4 treatments. 2. anaemia produced several changes in radioiodinated proteins. 3. sialoglycoprotein radiolabelling was unchanged, even with over 90% reticulocytosis. 4. decreased periodate/nab3h4 ... | 1982 | 7083822 |
| antimalarials. 14. 5-(aryloxy)-4-methylprimaquine analogues. a highly effective series of blood and tissue schizonticidal agents. | a series of five 5-(aryloxy)-4-methylprimaquine analogues has been prepared and evaluated for antimalarial activity. the compounds were tested for suppressive activity against plasmodium berghei in mice and for radical curative activity against plasmodium cynomolgi in the rhesus monkey. the compounds were not only significantly superior to primaquine as radical curative agents but also were suprisingly highly effective as suppressive agents. | 1982 | 7131488 |
| monoclonal antibodies that protect in vivo against plasmodium chabaudi recognize a 250,000-dalton parasite polypeptide. | twenty monoclonal antibodies have been prepared to the erythrocytes from cba/ca mice infected with the rodent malaria plasmodium chabaudi. by immunofluorescence, 15 of these antibodies recognized parasite antigens expressed only during the development of mature trophozoites to schizonts and merozoites, 2 recognized parasite antigens that were expressed throughout most of the intraerythrocytic cycle, and 3 recognized the membranes of all infected and uninfected erythrocytes. by immunoprecipitatio ... | 1982 | 7141700 |
| the binding of antibodies from plasmodium berghei-infected rats to isoantigenic and parasite-specific antigenic sites on the surfaces of infected reticulocytes. | ferritin-labelling techniques at the ultrastructural level have shown that antiserum from august rats immune to p. berghei infection contains antibodies which bind to the surfaces of parasitized reticulocytes but not to uninfected cells. two antibody specificities have been demonstrated by comparing antisera i absorbed with infected reticulocytes, ii absorbed with uninfected reticulocytes, and iii unabsorbed. ferritin labeling was much increased with antiserum preabsorbed with uninfected reticul ... | 1982 | 7036051 |
| plasmodium berghei: architectural analysis by freeze-fracturing of the intraoocyst sporozoite's pellicular system. | 1982 | 7037440 | |
| antibodies to the protective antigen of plasmodium berghei sporozoites prevent entry into cultured cells. | 1982 | 7037957 | |
| mesangial disposal of glomerular immune deposits in acute malarial glomerulonephritis of rats. | the disposal of immune complexes by the glomerulus and the participation of infiltrating monocytes were studied in acute malaria-associated glomerulonephritis. young sprague-dawley rats were infected with plasmodium berghei. parasitemia reached a maximum after 8 to 12 days, ending by day 20. in all infected rats, renal immunofluorescence microscopy showed in all glomeruli granular deposition of rat igg, igm, and c3 in a mesangial distribution. the staining was strongest from days 8 to 12, then d ... | 1982 | 7038293 |
| identification of a schizont- and species-specific surface glycoprotein on erythrocytes infected with rodent malarias. | erythrocytes infected with mature trophozoites of plasmodium chabaudi and reticulocytes infected with p. berghei were labelled metabolically in vitro with [35s]methionine. the labelled cells were incubated with normal and immune serum and washed to remove unbound antibody. solubilisation of the antibody-coated cells in detergent was followed by co-precipitation of antibody/antigen complexes and analysis of the immunoprecipitates by sds-page and fluorography. one major parasite polypeptide of 250 ... | 1982 | 7038481 |
| chronic, patent plasmodium berghei malaria in splenectomized mice. | it was shown that splenectomized mice could develop a certain resistance to plasmodium berghei but usually no real immunity, since the infection became chronic, often with high parasitemias. a patent infection lasting at least 2 weeks was necessary for the development of this degree of protection. prolonged suppression to subpatent levels (sulfonamide treatment), rather than radical cure (chloroquine), after 2 weeks of patency yielded a higher proportion of mice resistant to superinfection. an i ... | 1982 | 7040249 |
| lymphocyte traffic and lymphocyte destruction in murine malaria. | normal lymphocytes labelled with 51cr were injected into mice at various stages of lethal and non-lethal malaria infections. marked alterations were seen in the uptake into spleen and liver, which correlated with the outcome of the infection. non-lethal infections and lethal infections in mice protected by vaccination caused increased uptakes, especially in the liver. in lethal infections, particularly plasmodium berghei, uptakes were below normal values at certain times: this was apparently due ... | 1982 | 7042547 |
| inhibition of antibody-binding as a radioimmunoassay for plasmodium berghei infection in rats. | sonicated red blood cells of rats infected with plasmodium berghei (pb) were used to coat plastic tubes, which were subsequently tested for capacity to bind anti-p, berghei antibodies. binding was detected using radioiodinated staphylococcus protein a. two antigenic preparations were used to make the solid-phase adsorbent (and also as inhibitors in the inhibition tests): sonicated red blood cells (sirc), and a supernate of frozen-thawed, infected red blood cells (fte). treatment of the antigen-c ... | 1982 | 7042946 |
| infectivity of in vitro-formed plasmodium berghei ookinetes to mosquitoes. | 1982 | 7042949 | |
| superoxide dismutase (sod) in mouse red blood cells infected with plasmodium berghei. | 1982 | 7042950 | |
| correlation between susceptibility to malaria and babesia parasites and to endotoxicity. | adult (185g) rats are about twice as sensitive to the harmful effects of injected endotoxin as are younger (65g) rats. this relationship correlates with an earlier report on the densities of plasmodium berghei at which deaths occur in rats of these two age groups. similarly lizards, which withstand very high parasitaemias of malaria parasites, are refractory to very large doses of endotoxin. this correlation appears to hold for malaria and babesiosis in all host species for which information is ... | 1982 | 7043806 |
| comparative studies on the immunogenicity of infective and attenuated sporozoites of plasmodium berghei. | 1982 | 7043807 | |
| plasmodium berghei: isolation and maintenance of an irradiation attenuated strain in the nude mouse. | 1982 | 7044811 | |
| corticosterone regulation of the effector function of malarial immunity during pregnancy. | in the experimental plasmodium berghei mouse model, as in human malaria, reduced maternal responsiveness and even loss of immunity were observed during pregnancy. loss of immunity in the second half of pregnancy occurred during a period of elevated plasma corticoid levels. further analysis showed that plasma corticoid levels were significantly higher in immunodepressed mice than in mice that remained immune throughout pregnancy. plasma corticosterone levels differed increasingly from those in mi ... | 1982 | 7044972 |
| effects of malaria (plasmodium berghei) on the maternal-fetal relationship in mice. | plasmodium berghei infection was more severe in pregnant than in nonpregnant mice. infection initiated on gestation day 7 resulted in rapidly increasing parasitemia and deaths of all pregnant mice within 12 days, while some nonpregnant mice survived until day 21 postinfection. when mice were infected on gestation day 12 or 14, a proportion of mice died before parturition; but some animals survived to deliver living pups. reduced birthweights and increased spleen weight to body weight ratios were ... | 1982 | 7045348 |
| interactions between the intestinal flagellates giardia muris and spironucleus muris and the blood parasites babesia microti, plasmodium yoelii and plasmodium berghei in mice. | in mice infected with the intestinal flagellates giardia muris or spironucleus muris, together with the blood parasites babesia microti or plasmodium yoelii, there is a temporary decrease of flagellate cyst output coincident with the peak of the blood parasite infections, followed by a rapid return to normal levels. this decrease in cyst output is correlated with decreased numbers of trophozoites in the small intestine. the effect on s. muris is more marked than that on g. muris. neither blood p ... | 1982 | 6214756 |
| kinetics of immunosuppression of sporozoite-induced immunity by mycobacterium bovis bcg. | the data reported in this study demonstrate that the vaccination of nih/nmri mice with viable mycobacterium bovis bcg organisms induces a state of immunosuppression that renders the recipient animals incapable of a protective immune response to the malaria sporozoite vaccine. the expression of this altered protective immune response is dependent upon the dosage of the two live vaccines, as well as upon the sequence of their administration. data presented here show that the skin test responses (a ... | 1982 | 6215354 |
| inhibition of idiotype--anti-idiotype interaction for detection of a parasite antigen: a new immunoassay. | described in this report is an immunoradiometric assay of general applicability that is based on a new principle: the inhibition of the interaction between monoclonal antibodies by an antigen. the advantages of this assay are that it measures concentrations of single epitopes, purified antigen is not required, and the reagents can be obtained in unlimited amounts and are homogeneous. its features are particularly attractive when the antigen has not been purified and is a minor component of a com ... | 1982 | 6122269 |
| retention of plasmodium berghei sporozoites within perfused mouse livers. | a mouse liver perfusion model was adapted to evaluate the efficiency of the liver in retaining plasmodium berghei sporozoites. specific numbers of sporozoites were perfused into each liver via a portal vein cannula. the numbers of sporozoites in the perfusate effluent were counted and the percent sporozoite retention calculated. over 95% of sporozoites suspended in medium with plasma were retained in a normal liver following a single passage. sporozoites were seen in sinusoids of perfused livers ... | 1982 | 6122362 |
| production of monoclonal antibodies by hybridomas sensitized to sporozoites of plasmodium berghei. | hybridoma cell lines, which secreted antibodies directed against either the surface of plasmodium berghei sporozoites or mosquito debris, were produced by fusion of spleen cells of p. berghei sporozoite-immunized mice with p3-x63-ag8 myeloma cells. four cloned antibody-secreting cell lines were successfully established. two of these clones (f9 and g10) were obtained from the fusion of spleen cells from mice that had undergone two immunizations. these clones produced an igm antibody that did not ... | 1982 | 6129292 |
| plasmodium berghei: immunologically active proteins on the sporozoite surface. | 1982 | 6174361 | |
| the use of percoll gradients, elutriator rotor elution, and mithramycin staining for the isolation and identification of intraerythrocytic stages of plasmodium berghei. | intraerythrocytic parasites of plasmodium vinckei and plasmodium berghei were separated according to their developmental stages using discontinuous percoll gradients. contaminating nucleated blood cells such as leukocytes were removed by elutriation centrifugation. the stages were unequivocally identified in smears using a newly developed dna-specific staining procedure with mithramycin and fluorescence microscopy. this stain can also be used to detect parasites in human blood of very low parasi ... | 1982 | 6177116 |
| [protective action of specific gamma globulin against malaria caused by plasmodium berghei]. | 1982 | 6179147 | |
| approaches to assessing host resistance. | there is increasing evidence that chronic, subclinical exposure to certain environmental pollutants may upset immune responsiveness and alter susceptibility of animals to infectious agents. environmental chemicals or drugs may affect diverse aspects of the immune system, leading to immunosuppression, immunopotentiation, hypersensitivity or perturbed innate host resistance. a variety of infectious models is available that involves relatively well defined target organs and host defense mechanisms; ... | 1982 | 6277617 |
| mechanism of diethyl-dithiocarbamate induced elevation of parasitemia in plasmodium berghei infection. | 1982 | 6282744 | |
| myristate-induced release of superoxide and hydrogen peroxide from peritoneal macrophages in mice immunized to toxoplasma gondii and plasmodium berghei. | 1982 | 6290731 | |
| synthesis and biological properties of 2'-deoxy-5-vinyluridine and 2'deoxy-5-vinylcytidine. | rapid and efficient syntheses for the preparation of 2'-deoxy-5-vinyluridine and 2'-deoxy-5-vinylcytidine are described starting from nucleoside precursors. contrary to some previous reports, 2-deoxy-5-vinyluridine has be found to be quite stable under normal laboratory conditions but when tested in animals shows neither toxicity nor anti-leukemic (l1210 cells) or anti-parasitic (plasmodium berghei) activity. 2'-deoxy-5-vinylcytidine appears to polymerise readily. it is much less toxic to cell c ... | 1982 | 6292837 |
| chemiluminescence response of peritoneal macrophages to parasitized erythrocytes and lysed erythrocytes from plasmodium berghei-infected mice. | the chemiluminescence response of normal mouse peritoneal macrophages to parasitized erythrocytes isolated from mice 3 weeks after infection with plasmodium berghei was examined. only 4 of 12 animals showed positive responses, whereas 8 showed negative responses. photomicrographs revealed that only in chemiluminescence-positive animals were parasitized erythrocytes attached to or phagocytized by macrophages. when lysed parasitized-erythrocyte cell suspensions were added to the peritoneal macroph ... | 1982 | 6749686 |
| virulent p. berghei malaria: prolonged survival and decreased cerebral pathology in cell-dependent nude mice. | the course of lethal plasmodium berghei infection was examined in nu/+ and t cell-deficient nu/nu balb/c mice. a rapidly fatal neurologic syndrome, including ataxia, hemiparesis, and seizures, was seen in the nu/+ mice early in the infection, whereas this syndrome was absent in the nu/nu mice. the nu/nu mice also developed anemia more slowly, had lower levels of immune complexes and total igg, and had smaller decreases in serum c3 compared with the nu/+ mice. histopathologic examination of the b ... | 1982 | 6749988 |
| fansidar prophylaxis, therapy, and immune responses in rodent malaria (plasmodium berghei). | in order to determine the effect of fansidar on plasmodial infection in mice, outbred, adult, swiss-webster mice were treated with fansidar (20 mg sulfadoxine and 1 mg pyrimethamine/kg body weight) at various intervals before and/or after inoculation with blood stages of plasmodium berghei. drug therapy resulted in cure if it was given before the parasitemia rose to 53%. oral administration of fansidar was more effective in reducing or preventing parasitemia than intramuscular injection. fatal i ... | 1982 | 6750070 |
| antimalarials. 13. 5-alkoxy analogues of 4-methylprimaquine. | a series of nuclear and side-chain analogues of 4-methylprimaquine incorporating an alkoxy group in the 5-position of the quinoline nucleus has been prepared. the compounds were tested for suppressive antimalarial activity against plasmodium berghei in mice and for radical curative antimalarial activity against plasmodium cynomolgi in the rhesus monkey. although the toxicity problems characteristic of the 8-aminoquinolines were not overcome, several of the compounds, surprisingly, were highly ef ... | 1982 | 6750123 |
| the chemotherapy of rodent malaria xxxiii. the activity of chloroquine and related blood schizontocides and of some analogues in drug-induced pigment clumping. | 1982 | 6751248 | |
| plasmodium berghei: uptake and distribution of chloroquine in infected mouse erythrocytes. | 1982 | 6751843 | |
| inhibition by cyclosporin a of rodent malaria in vivo and human malaria in vitro. | the development and course of normally lethal parasitemias in mice inoculated intraperitoneally with erythrocytic stages of plasmodium yoelii or plasmodium berghei were markedly affected by treatment with the antilymphoid drug cyclosporin a (cs-a). when the first of four daily subcutaneous 25-mg/kg doses of cs-a was given at the time of parasite inoculation, patent infections failed to develop. if begun up to 5 days earlier, this same treatment regimen prolonged the prepatent period, attenuated ... | 1982 | 6752020 |
| plasmodium berghei malaria: blockage by immune complexes of macrophage receptors for opsonized plasmodia. | immune complexes produced in vitro by mixing immune serum and soluble plasmodium berghei antigens and immune complexes precipitated from serum of acutely infected rats blocked macrophage receptor sites for opsonized plasmodia. the immune complexes were precipitated from acute-phase serum, using polyethylene glycol, and their composition was determined by using a raji cell immunofluorescence assay. the immune complexes contained immunoglobulin g (igg), igm, and malarial antigens. these results in ... | 1982 | 6752023 |
| differential sensitivity in vivo of lethal and nonlethal malarial parasites to endotoxin-induced serum factor. | sera from mice infected with plasmodium yoelii or plasmodium berghei and given endotoxin contained nonspecific mediators which killed both species of parasite and tumor cells in vitro. the sera resembled tumor necrosis sera obtained from mice given macrophage-activating agents such as propionibacterium acnes (formerly designated corynebacterium acnes) or mycobacterium bovis bcg and then endotoxin. cytotoxicity developed parallel to parasite killing activity and indicated that macrophages were ac ... | 1982 | 6752029 |
| direct infection of hepatocytes by sporozoites of plasmodium berghei. | to identify the unknown liver cell type initially invaded by sporozoites of mammalian malaria, young rats were inoculated intravenously with large numbers of plasmodium berghei sporozoites obtained from infected anopheles stephensi mosquitoes. fine structural studies of liver specimens obtained from the rats within 2 min after inoculation demonstrated the presence of morphologically unaltered sporozoites in the cytoplasm of hepatocytes. many sporozoites were also observed undergoing cytolysis wi ... | 1982 | 6752394 |
| the demonstration of plasmodium berghei sporozoites in rat hepatocytes one hour after inoculation. | 1982 | 6753390 | |
| cellular changes in bone marrow of malaria-infected mice. iii. chemotaxis of granulocytes. | the number of bone marrow cells and their chemotactic activity was studied during malaria infection. two days after infection of balb/c mice with plasmodium berghei, an increase in granulocyte number was observed in the blood. a modified boyden chamber chemotaxis assay was employed to investigate the mechanism of granulocyte accumulation in the blood. bone marrow cells from normal mice, from mice during a primary lethal infection and from immune mice after challenge were compared. the complement ... | 1982 | 6753391 |
| 2-acetylpyridine thiosemicarbazones. 4. complexes with transition metals as antimalarial and antileukemic agents. | reaction of the 2-acetylpyridine thiosemicarbazones, 3-azabicyclo[3.2.2]nonane-3-thiocarboxylic acid 2-[1-(2-pyridyl)ethylidene]hydrazide (iiia), its selenium analogue (iiib), 1h-hexahydroazepine-1-thiocarboxylic acid 2-[1-(2-pyridyl)ethylidene]hydrazide (iv), and 1h-octahydroazocine-1-thiocarboxylic acid 2-[1-(2-pyridyl)ethylidene]hydrazide (v) with cu(ii), ni(ii), fe(iii), and mn(ii) salts gave crystalline complexes. relative to the free ligands, these complexes show reduced antimalarial activ ... | 1982 | 6754934 |
| susceptibility of plasmodium berghei in a laboratory population of anopheles atroparvus (diptera: culicidae) after the introduction of plasmodium-refractory genotypes. | 1982 | 6754936 | |
| ferriprotoporphyrin ix binding substances and the mode of action of chloroquine against malaria. | bovine serum albumin and preparations of cell sap from malaria parasites and normal erythrocytes were tested for ability to protect cellular membranes against the toxicity of ferriprotoporphyrin ix (fp) and a chloroquine-fp complex. suspensions of plasmodium berghei (approximately 7 x 10(6) parasites per ml, isolated from saponin-lysed, infected erythrocytes) were used as a test system. toxicity was monitored by measuring changes in turbidity of these suspensions at 700 nm. parasite cell sap (0. ... | 1982 | 6755119 |
| degenerating exo-erythrocytic forms of plasmodium berghei in rat liver: an ultrastructural and cytochemical study. | morphological and cytochemical aspects of the host response to almost mature exo-erythrocytic forms (eef) of plasmodium berghei in rat hepatocytes were studied by electron microscopy. young stages (less than 47 h) never evoked a local reaction. two types of nearly mature eef (47-51 h) could be distinguished, normal (eef type i) and those that were the target of infiltrating cells (eef type ii). the latter were in a stage of early or advanced degeneration and generally exhibited increased electro ... | 1982 | 6755366 |
| adoptive transfer of immunity to plasmodium berghei with spleen cells from drug cured mice. | plasmodium berghei infection gives rise to a fatal fulminating parasitaemia in mice. resistance to the infection can be achieved if the mice are allowed to recover from the blood-induced infection by administration of chloroquine. in cba/caj, one cycle and in balb/c mice, at least, two cycles of infection, alternating with drug-cure were necessary for the establishment of immunity. no parasitaemia was seen following further challenges after the third infection. adoptive transfer of spleen cells ... | 1982 | 6755741 |
| effect of multiple sporozoite challenges on immunity to malaria. | 1982 | 6755744 | |
| androgen suppression of circulating immune complexes and enhanced survival in murine malaria. | 1982 | 6757933 | |
| detection of exoerythrocytic stages of plasmodium berghei in fixed liver tissue and cultured cells by an immunoperoxidase antibody technique. | an immunoperoxidase antibody (ipa) technique is described for the detection of exoerythrocytic (ee) stages of plasmodium berghei in rat liver sections or cultured w138 cells. ee stages were detected in liver sections 12 hours after inoculation of sporozoites, but as early as three hours after the inoculation of w138 cells. by 42 hours in liver sections and 48 hours in w138 cells, ee parasites were easily visible by low power microscopy, and merozoites were clearly seen by 72 hours within ee para ... | 1982 | 6758221 |
| loss of infectivity for swiss mice of a mefloquine-resistant strain of plasmodium berghei. | a strain of plasmodium berghei made resistant to a 244 mg/kg daily dose of mefloquine by interrupted subcurative therapy in successive passages in weanling rats, was maintained for more than six months under drug pressure and was cryopreserved. experiments with this strain have shown its loss of virulence for intact mice. in splenectomized swiss mice infection with this resistant strain is transient. the mefloquine resistance of this line was unstable and the strain became sensitive to mefloquin ... | 1982 | 6758223 |
| [development of pyronaridine-resistance in plasmodium berghei]. | 1982 | 6758485 | |
| [synthesis of some 2, 6, 9-substituted thioguanine and their antimalarial activity]. | 1982 | 6758486 | |
| [biochemical method of determining the chloroquine resistance of p. berghei in vitro]. | 1982 | 6759900 | |
| effects of immune and hyperimmune serum on the dynamics of phagocytosis of plasmodium berghei-infected erythrocytes. | 1982 | 6760349 | |
| characterization of plasmodium berghei antigens inducing blast transformation in immune rat lymphocytes. | rat erythrocytes, infected with plasmodium berghei, were disrupted by freezing and thawing or parasites were released by mechanical breaking of the agglutinated erythrocytes. the released soluble antigens, inducing blast transformation in non-adherent immune rat spleen cells, were fractionated by a sequence of salting out, ion exchange chromatography and gel filtration. at the final step a purification factor of 83.7-fold was achieved. this fraction contained three components discernible by sds- ... | 1982 | 6760572 |
| in vitro assessment of 2-acetylpyridine thiosemicarbazones against chloroquine-resistant plasmodium falciparum. | a series of 2-acylpyridine thiosemicarbazones was evaluated in vitro against a chloroquine-resistant plasmodium falciparum strain. antimalarial activity was assessed by the inhibition of uptake of [g-3h]hypoxanthine by the parasites. among the mono- and disubstituted derivatives tested, 13 of 17 had 50% inhibitory doses of less than 10 ng/ml. increasing the size of the ring at n4 from four to five, six, and seven members produced concomitant decreases in activity. similarly, increasing the size ... | 1982 | 6760808 |
| antimalarial activity of floxacrine (hoe 991) ii: studies on causal prophylactic and blood schizontocidal action of floxacrine and related dihydroacridinediones against plasmodium yoelii and p. berghei. | 1982 | 6760821 | |
| nmr studies of malaria. 31p nuclear magnetic resonance of blood from mice infected with plasmodium berghei. | high resolution 31p-nmr has been used for the non-invasive observation of metabolites and metabolic rates in blood of normal mice and of mice infected with plasmodium berghei, the causative agent of malaria. 31p-nmr was used to quantitate levels of 2,3-diphosphoglycerate in whole cells as a function of the degree of parasitemia and yielded good agreement with the results of enzymatic assays. the time-dependence of 31p metabolites was monitored in both normal and infected erythrocytes, greater ra ... | 1982 | 6760901 |
| effect of dichroa febrifuga on plasmodium berghei. | 1982 | 6762348 | |
| [interactions of 2 associated parasitoses in the rat: plasmodium berghei and strongyloides ratti]. | when plasmodium berghei and strongyloides ratti are associated, each one of these parasites can modify development of the other one. this repercussion is subordinated to the respective periods of contamination and strength of influential parasitism. it lies in an inhibition of protozoosis or intensification of helminthiasis. when severe, palustral infection releases two hypercosticosteronemy phases; the more important one is situated at the peak of the parasitemia. these hypercorticosteronemiae ... | 1982 | 6762842 |
| adenosine triphosphate levels in mouse erythrocytes infected with chloroquine-sensitive and chloroquine-resistant plasmodium berghei. | 1982 | 6763504 | |
| [studies on piperaquine as long-acting antimalarial drug against plasmodium berghei in mice]. | 1982 | 6763837 | |
| the effect of cryopreservation on gametocytogenesis of plasmodium berghei berghei: a preliminary report. | 1982 | 6765166 | |
| [plasmodium berghei: antibody response and protective immunity induced by vaccination]. | the authors have studied the behaviour of swiss mice and of 5 inbred strains of mice in order to investigate: the protective effect, in the homologous infection test, of six vaccine inoculations of irradiated parasites belonging to two strains of plasmodium berghei: istisan and k173; the capacity to produce humoral antibodies after vaccine treatments and during infection; the probable correlation between the high antibody titre and the protection against infection. the results of the present stu ... | 1982 | 6765341 |