Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| entamoeba histolytica: target cells killed by trophozoites undergo dna fragmentation which is not blocked by bcl-2. | amebic destruction of neutrophils and macrophages is contact-dependent. adherence is mediated by a galactose-specific surface lectin on the amebic membrane. the pathway by which contact-dependent cytolysis of the target cell occurs is unknown. we hypothesized that target cell death is due to the triggering of apoptosis (programmed cell death) by the amebae. the purpose of this study was to determine whether target cell dna is fragmented into a ladder pattern characteristic of apoptosis and to te ... | 1994 | 7957763 |
| characterization of two distinct gene transcripts for ribosomal protein l21 from pathogenic and nonpathogenic strains of entamoeba histolytica. | a second gene (rp-l21) copy, clone g34, coding for ribosomal (r-) protein l21, was isolated from the pathogenic (p) strain hm-1:imss cl6 of the intestinal parasite entamoeba histolytica (eh). the gene was compared to the previously isolated copy, gle3 [petter et al., mol. biochem. parasitol. 56 (1992) 329-334], with respect to its primary structure, mrna levels and binding to the r-complex during translation. unlike the gle3 gene copy [petter et al., mol. biochem. parasitol. 56 (1992) 329-334], ... | 1994 | 7959049 |
| [microbiological diagnosis of malaria and amoebiasis]. | all stages of malaria and amebiasis may be sensitively and specifically diagnosed by means of microbiological methods. microscopical parasitology holds a key position when supplemented by immunodiagnostic assays. molecular biology techniques are currently still inferior to the above procedure. tests for the differentiation between pathogenic and nonpathogenic entamoeba histolytica (entamoeba dispar) require further simplification before generally applicable. | 1994 | 7959857 |
| [therapy for malaria and amoebiasis]. | treatment of malaria depends on the infecting plasmodium species. in plasmodium falciparum malaria the treatment also depends on whether chloroquine resistances occur and whether the course is uncomplicated or complicated. uncomplicated cases are cared for with chloroquine and with mefloquine or halofantrine when the patient comes from areas with chloroquine resistances. patients with complicated plasmodium falciparum malaria must get chinine and doxycycline. a careful fluid balance is extremely ... | 1994 | 7959858 |
| detection of ubiquinone in parasitic and free-living protozoa, including species devoid of mitochondria. | ubiquinone (coenzyme q, coq) was analyzed and individual homologues quantified in 11 species of parasitic and free-living protozoa by a combination of thin-layer chromatography and high performance liquid chromatography. fast atom bombardment ionization-mass spectrometry was used for the first time to confirm the identity of the fractionated coq homologues and proved to be a fast, gentle and convenient method for ubiquinone identification. ubiquinone was detected in all organisms including those ... | 1994 | 7969263 |
| purification and molecular characterization of the nad(+)-dependent acetaldehyde/alcohol dehydrogenase from entamoeba histolytica. | a bifunctional 95 kda polypeptide (ehadh2) harbouring acetaldehyde dehydrogenase and alcohol dehydrogenase activities was purified to homogeneity from trophozoite extracts of the protozoan parasite entamoeba histolytica. kinetic studies revealed that the enzyme utilizes nad+ rather than nadp+ as cofactor. km values for acetyl-coa, acetaldehyde and ethanol were found to be 0.015, 0.15 and 80 mm respectively in the presence of 0.2 mm nad+. the primary structure of ehadh2 as deduced from respective ... | 1994 | 7980441 |
| [gnathostomiasis after a stay in thailand]. | during a 4-week holiday in thailand a 13-year-old boy from germany briefly fell ill with diarrhoea without blood-tinged stools and fever. 5 months later oedematous, reddish swellings occurred, at first on the right upper arm, later over the right side of the chest. especially at night these swellings were associated with severe local and generalized itching. bilaterally the axillary lymph-nodes were hard and slightly enlarged. there was a marked eosinophilia of 56%, the white count was 22,700/mi ... | 1994 | 7982372 |
| monoclonal antibody-based immunohistochemical demonstration of entamoeba histolytica in liver tissues of experimentally infected hamster (mesocricetus auratus). | histopathological changes and the presence of entamoeba histolytica trophozoites was sequentially followed after intrahepatic inoculation of the parasites in 42 hamsters, 35 of which received no treatment whereas the remaining seven were treated with metronidazole. the liver tissues were examined for amoebic trophozoites by a monoclonal antibody (mab)-based immunofluorescence assay (ifa), a mab-based immunoperoxidase (ipx) and h & e staining. the number of hamsters developing abscesses was incre ... | 1994 | 7982753 |
| expression and characterization of a rac family protein kinase of entamoeba histolytica. | we previously reported the isolation from entamoeba histolytica of a novel rac family protein kinase gene, termed ehrac1, for "related to camp-dependent protein kinases and protein kinase cs". to study the function and properties of this kinase gene further, we fused the full-length coding region and the truncated catalytic domain of the ehrac1 gene in frame with the gene encoding glutathione s-transferase in the pgex-kg vector and expressed the fusion in escherichia coli. the thrombin-cleaved a ... | 1994 | 7984173 |
| primary structure of the entamoeba histolytica gene (ehvma1) encoding the catalytic peptide of a putative vacuolar membrane proton-transporting atpase (v-atpase). | 1994 | 7984182 | |
| an intron-containing gene coding for a novel 39-kilodalton antigen of entamoeba histolytica. | 1994 | 7984185 | |
| cryptosporidiosis among medical patients with the acquired immunodeficiency syndrome in tikur anbessa teaching hospital, ethiopia. | fresh stool specimens, collected at random from 63 medical in-patients with acquired immunodeficiency syndrome (aids), were studied prospectively for cryptosporidium oocyst. the diagnosis of aids was made according to the clinical case definition of the bangui criteria. these patients presented with profuse watery diarrhoea, significant weight loss and other associated symptoms and signs of clinical manifestations of symptomatic human immunodeficiency virus (hiv) infection. using the modified ki ... | 1994 | 7835259 |
| enteropathogens associated with acute diarrhoeal diseases. | five types of escherichia coli are responsible for as much as 25% of all diarrheal diseases in developing countries. they tend to be transmitted via contaminated foods, particularly weaning foods, and water. they include enterotoxigenic, enteropathogenic, enteroadherent, enteroinvasive, and enterohemorrhagic e. coli. shigella species are responsible for 10-15% of acute diarrheas in children less than 5 years old and the most common etiologic agents of childhood dysentery. shigellosis is common i ... | 1994 | 7835992 |
| identification of a developmentally regulated transcript expressed during encystation of entamoeba invadens. | differentiation of trophozoites into cysts in entamoeba species has been described morphologically and to a lesser extent biochemically, but studies of stage specific gene expression have not been reported. at present entamoeba invadens is the only species that can be induced to differentiate in axenic culture and is a useful model system for the human parasite entamoeba histolytica. using this model system, we performed cdna-mrna hybridization experiments to compare the rna populations from tro ... | 1994 | 7838173 |
| standardization and evaluation of elisa for the serodiagnosis of amoebic liver abscess. | an elisa test for the serological diagnosis of amoebic liver abscess (ala) was standardized and evaluated in sera from three groups of patients: (1) three patients with diagnosis confirmed by isolation of the parasite, (2) thirty seven patients with diagnosis established by clinical findings and ultrasound studies and (3) seven patients whose diagnosis were established by clinical findings and a positive double immunodifusion test. ninety one serum samples from healthy subjects and 22 from patie ... | 1994 | 7823821 |
| intestinal parasitoses in the kandy area, sri lanka. | the prevalence of intestinal protozoa and geohelminths was assessed among two diverse populations in the kandy area: adults attending medical outpatients clinics at the teaching hospital peradeniya, and pre-school children in low-cost housing areas within the kandy municipality. in addition to a brief history and examination, a fresh stool sample was obtained and examined by direct smears in saline and iodine, and by formol-ether concentration. the children's stool samples were also examined for ... | 1994 | 7777909 |
| blastocystis hominis infection, a cause of human diarrhea. | blastocystis hominis has long been described as a non pathogenic protozoan parasite until recently when claims have been made that it can result in pathogenic conditions. of the 729 stool samples (614 from survey and 115 from pediatric wards) examined, 18.1% of them were found to be positive for one or more intestinal protozoan cyst. the commonest was giardia intestinalis (8.4%) followed by entamoeba coli (7.1%) and entamoeba histolytica (5.1%) in the normal children without symptoms of diarrhea ... | 1994 | 7777913 |
| [recent progress in the diagnosis of intestinal amebiasis. contribution of molecular biology]. | only 10% of entamoeba histolytica strains have been reported to have a pathogenic capacity. theoretically and under certain conditions, confirming this estimation should limit the number and the cost of the treatments administered to the four hundred million asymptomatic carriers in the world. several new routine tests which allow more accurate and easier diagnosis of intestinal amoebiasis and in particular the differential diagnosis between the pathogenic and nonpathogenic strains are or soon w ... | 1994 | 7802347 |
| intestinal amebiasis: histopathologic features in experimentally infected mice. | intestinal amebiasis is still an important health problem in developing regions of the world. in order to advance our knowledge on the pathogenesis and to test the relevance of recently obtained in vitro observations, suitable in vivo experimental models of intestinal amebiasis are needed. in the past a variety of laboratory animals have been used, but the mouse, whose genetics and immunology is well known, has been seldomly used. therefore, entamoeba histolytica strain hm1:imss was directly ino ... | 1994 | 7803977 |
| antiprotozoal activities of benzimidazoles and correlations with beta-tubulin sequence. | benzimidazoles have been widely used since the 1960s as anthelmintic agents in veterinary and human medicine and as antifungal agents in agriculture. more recently, selected benzimidazole derivatives were shown to be active in vitro against two protozoan parasites, trichomonas vaginalis and giardia lamblia, and clinical studies with aids patients have suggested that microsporidia are susceptible as well. here, we first present in vitro susceptibility data for t. vaginalis and g. lamblia using an ... | 1994 | 7811023 |
| survey of the prevalence of intestinal parasites in children of primary school age. | a survey of the prevalence of intestinal parasites in primary school children was carried out in nimo in njikoka local government area of anambra state, nigeria. stool samples were taken from children belonging to eight (8) primary schools in the area. of the 1,536 stool samples examined using light microscopy after formalether centrifugation, 922 (60%) harboured one or more parasites. the prevalence of the parasites was as follows: ascaris lumbricoides (20.8%); trichuris trichiura, (15.3%); nec ... | 1994 | 7756189 |
| primary structure of an entamoeba histolytica nicotinamide nucleotide transhydrogenase. | 1994 | 7739679 | |
| the etiology of early childhood diarrhea: a community study from guinea-bissau. | a potential enteropathogen was found in 50% of 1219 diarrheal episodes and 48% of 511 asymptomatic controls in a 1-year community study of childhood diarrhea. rotavirus (3% of episodes), cryptosporidium species (6%), and enteropathogenic escherichia coli (epec) with epec adherence factor (4%) were more prevalent in cases than controls. giardia lamblia (19%) was the most prevalent organism but was not associated with diarrhea. enterotoxigenic e. coli (12%), strongyloides stercoralis (5%), shigell ... | 1994 | 8158030 |
| parasitic infection among children living in two orphanages in cairo. | children living in institutions as orphanages are more exposed to intestinal parasitism, since crowding and behavioral pattern contribute greatly to the spread of parasitic infection. the present study was conducted on 100 children living in ain-shams and el-mowassa orphanages, cairo. twenty children living under appropriate health conditions were studied as controls. ages of both groups ranged from 6-12 years. the results of this study revealed that 69/100 (69%) orphanage children were positive ... | 1994 | 8169435 |
| the prevalence of infection by intestinal parasites in an urban slum in são paulo, brazil. | sedimentation parasitological examinations were performed in stool specimens from a random household-based sample of 407 dwellers, all age groups being represented, in a slum of são paulo, south-eastern brazil. intestinal parasites were detected in 45.7% of the specimens. the most prevalent parasites in this population were ascaris lumbricoides (23.8%) and trichuris trichiura (17.2%); only 17 individuals (4.2%) harboured giardia duodenalis and 6 (1.5%) harboured entamoeba histolytica. taenia and ... | 1994 | 8170003 |
| a study of the aetiological agents of childhood diarrhoea in lagos, nigeria. | from december 1989 to may 1990, 315 faecal samples from children under 5 years old with diarrhoea (215) and without diarrhoea (100) seen at paediatric clinics were investigated for bacterial, viral and parasitic enteropathogens. standard and recently described methods were used for the investigations, which revealed that 74.9% of children with diarrhoea were infected with enteropathogens compared with 28% of controls. in the diarrhoeal group, 59.1% had a bacterial, 26.5% a viral and 2.3% a paras ... | 1994 | 8289209 |
| cloning and expression of the gene for an nadp(+)-dependent aldehyde dehydrogenase of entamoeba histolytica. | 1994 | 8183316 | |
| molecular cloning of an entamoeba histolytica gene encoding a putative mos family serine/threonine-kinase. | molecular cloning techniques were used to isolate an ameba gene encoding a putative 33-kda serine/thyronine-kinase. the open reading frame of this gene, called ehmfk1 for e. histolytica mos family kinase, shows a 24% positional identity with the human proto-oncogene mos, including 32 of 33 amino acids conserved in other serine/threonine-kinases. | 1994 | 8186259 |
| protection of gerbils from amebic liver abscess by immunization with a recombinant protein derived from the 170-kilodalton surface adhesin of entamoeba histolytica. | the protozoan parasite entamoeba histolytica causes extensive morbidity and mortality worldwide through intestinal infection and amebic liver abscess. here we show that vaccination of gerbils, a standard model for amebic liver abscess, with recombinant proteins derived from the 170-kda galactose-binding adhesin of e. histolytica and the serine-rich e. histolytica protein or a combination of the two recombinant antigens provides excellent protection against subsequent hepatic challenge with virul ... | 1994 | 8188384 |
| use of micro samples of finger prick blood dried on filter paper for a quick and simple dipstick dot-eia for diagnosis of amebic liver abscess (ala). | filter paper was used as a support to absorb micro samples of finger prick blood for detection of antibodies to entamoeba histolytica (causative organism of amebiasis) by a rapid dipstick dot-eia technique; 8 microliters of blood was sufficient to saturate discs (diameter 6 mm) of whatman paper no. 3. conditions of elution of blood from the discs were optimized and the best results were obtained when 0.4 ml of buffer was used for elution for 30 minutes at room temperature. the filter paper techn ... | 1994 | 8189328 |
| cloning and sequence determination of the gene coding for the pyruvate phosphate dikinase of entamoeba histolytica. | we isolated three overlapping clones from a dna genomic library of entamoeba histolytica strain hm1:imss, whose translated nucleotide (nt) sequence shows similarities of 51, 48 and 47% with the amino acid (aa) sequences reported for the pyruvate phosphate dikinases from bacteroides symbiosus, maize and flaveria trinervia, respectively. the reading frame determined codes for a protein of 886 aa. | 1994 | 8194759 |
| entamoeba histolytica: electron-dense granule secretion, collagenase activity and virulence are altered in the cytoskeleton mutant bg-3. | hm-1:imss, a pathogenic strain of entamoeba histolytica, and its mutant bg-3, identified by resistance to cytochalasin d, were tested for their capacity to: (i) secrete electron-dense granules; (ii) adhere and digest native type i collagen gels; and (iii) produce liver abscesses in new-born hamsters. the results demonstrate that the mutant has low adherence to collagen, low electron-dense granule secretion and collagenolytic activity, and low capacity to produce liver lesions in vivo, compared w ... | 1994 | 8196550 |
| amoebiasis: a rare cause of cardiac tamponade. | cardiac tamponade secondary to perforation of a hepatic amoebic abscess developed six years after the patient had visited an area where entamoeba histolytica is endemic. he was treated with metronidazole and imipenem, emergency percutaneous catheter drainage, and open surgical drainage. | 1994 | 8198889 |
| role of mucosal secretory immunity in the development of an amebiasis vaccine. | invasive colonic infection by the enteric protozoan entamoeba histolytica elicits a mucosal anti-amebic iga antibody response. the e. histolytica galactose-inhibitable adherence protein (giap) mediates parasite binding to colonic mucins and epithelial cells. anti-giap secretory iga antibodies are found during invasive amebiasis and can be elicited by immunization of rats with the native protein. entamoeba histolytica contains potent iga degradative activities due to its cysteine-specific proteas ... | 1994 | 8203722 |
| human secretory immunoglobulin a anti-entamoeba histolytica antibodies inhibit adherence of amebae to mdck cells. | the presence of secretory immunoglobulin a (iga) anti-entamoeba histolytica antibodies in the saliva of patients with intestinal amebiasis was demonstrated by immunoblot assay, and the capacity of these antibodies to inhibit amebic adherence to a monolayer of mdck cells was analyzed. inhibition was due to iga antiamebic antibodies and in part to anti-gal-binding-lectin antibodies, as demonstrated by absorption experiments with total amebic extract and with the fraction of gal-binding lectin. the ... | 1994 | 8300242 |
| is phagocytosis related to virulence in entamoeba histolytica schaudinn, 1903? | phagocytosis has been suggested as a marker of pathogenicity and virulence in entamoeba histolytica. irmgard montfort and ruy pérez-tamayo here review the evidence for this suggestion. | 1994 | 15275444 |
| pathogenicity, virulence and entamoeba histolytica. | 1994 | 15275497 | |
| pore formation and cytolysis by entamoeba histolytica. | 1994 | 15275556 | |
| entamoeba histolytica as a model for the primitive eukaryotic cell. | entamoeba histolytica is a structurally simple eukaryote lacking mitochondria, peroxisomes and a well-developed golgi apparatus, also in its biochemistry, it deviates substantially from the more complex eukoryotes. these features have alternatively been interpreted as archaic, ie. the ancestor of entamoeba branched off before the primitive eukaryotic cell obtained proto-mitochondria, or as regressive, ie. entamoeba has lost its mitochondria in the course of its adaptation to a parasitic life sty ... | 1993 | 15463662 |
| cell signalling and motility in entamoeba histolytica. | entamoeba histolytica crawls as a polarized cell following external stimuli, with the translocation of signals modifying extracellular matrix interactions and the amoeba cytoskeleton. nancy guillén here describes how the gliding of e. histolytica cells requires the activity of the actomyosin complex, and how actomyosin functions related to motility are necessary for pathogenesis and for amoebal escape from the host immune response. | 1993 | 15463670 |
| a new test for infection by entamoeba histolytica. | entamoeba histolytica is a common parasite of the human intestine, and in a significant percentage of cases it invades the tissues. virulent strains of the organism produce the proteinose histolysain, which almost certainly facilitates the invasive behaviour. histolysoin has been isolated, a selective assay developed, and specific antisera raised against the enzyme. as described here by alfredo luaces, lyda osorio and alan barren, the enzymeba test for infection by e. histolytica combines the sp ... | 1993 | 15463713 |
| surface properties, pathogenicity and differentiation of entamoeba histolytica. | 1993 | 15463760 | |
| encystation in entamoeba invadens. | knowledge of the biology and pathogenesis of the human parasite entamoeba histolytica has been limited by the lack of efficient procedures to induce axenic encystation in the laboratory. however, such methods have been developed for e. invadens, a reptilian parasite, for which encystation can be induced by mechanisms that are as yet poorly defined. this has allowed the analysis of some morphological, physiological and biochemical events that accompany differentiation into cysts. elucidation of t ... | 1993 | 15463765 |
| speciation and clonality in entamoeba histolytica. | 1993 | 15463785 | |
| prevalence of pathogenic intestinal parasites among preschool children in al-medina district, saudi arabia. | the prevalence of pathogenic intestinal parasites among preschool children in al-medina district, saudi arabia, was determined through a randomized multistage sampling of 8000 preschool children. duplicate specimens from each child were examined using a simple sedimentation technique. the overall prevalence of intestinal parasites among children screened was 18.4%. the most common parasite isolated was giardia lamblia (14.5%). this was followed by entamoeba histolytica (2.7%), enterobius vermicu ... | 1993 | 17590673 |
| cutaneous amebiasis in children. | a 7-month-old girl developed cutaneous amebiasis of the perianal and genital areas in association with amebic dysentery. the diagnosis was based on the identification of entamoeba histolytica by skin biopsy. she was treated with dehydroemetine and metronidazole with excellent results. this is only the eighth reported case of this disease in a child. | 1993 | 8302738 |
| the microbiology of acute diarrhoeal disease in the eastern province of saudi arabia. | a prospective study of acute diarrhoeal diseases in the eastern province of saudi arabia was carried out over a 19-month period to determine aetiology, risk factors and other epidemiological characteristics. of the 853 subjects studied, 344 were cases and 509 controls. more cases were seen in children than in adults. enteric pathogens were detected in 49% of the cases, but none in the controls. of the pathogens, 68% were bacterial with salmonella (34%) and shigella species (14.7%) being the most ... | 1993 | 8306900 |
| serologic reactivity to purified recombinant and native 29-kilodalton peripheral membrane protein of pathogenic entamoeba histolytica. | the 29-kda peripheral membrane protein of entamoeba histolytica has recently been demonstrated to have epitopes on pathogenic clinical isolates which were not detected by monoclonal antibodies on nonpathogenic isolates. to analyze the serological response to this protein, we tested 93 serum specimens (from 33 patients with amebic liver abscess, 7 patients with colitis, 2 patients with ameboma, 18 individuals harboring a nonpathogenic zymodeme strain, 10 healthy mexican migrant workers, and 23 he ... | 1993 | 8314979 |
| campylobacter-associated diarrhoea in egyptian infants: epidemiology and clinical manifestations of disease and high frequency of concomitant infections. | a diarrhoeal disease survey in alexandria, egypt determined the prevalence, seasonality, and household risk factors for campylobacter-associated diarrhoea in young children. the study population was 880 children (mean age = 9.8 months) presenting with diarrhoea at one of two hospitals. a control group consisted of 1,079 healthy children (mean age = 8.8 months) attending two nearby vaccination clinics. the overall isolation frequencies for campylobacter spp. were 16.8% for cases and 6.4% for the ... | 1993 | 8315255 |
| early branchings in the evolution of eukaryotes: ancient divergence of entamoeba that lacks mitochondria revealed by protein sequence data. | phylogenetic analyses of ribosomal rna sequences have played an important role in the study of early evolution of life. however, loomis and smith suggested that the ribosomal rna tree is sometimes misleading--especially when g+c content differs widely among lineages--and that a protein tree from amino acid sequences may be more reliable. in this study, we analyzed amino acid sequence data of elongation factor-1 alpha by a maximum likelihood method to clarify branching orders in the early evoluti ... | 1993 | 8315658 |
| 31p-nmr analysis of entamoeba histolytica. occurrence of high amounts of two inositol phosphates. | perchloric-acid extracts of axenic entamoeba histolytica were investigated by 31p-nmr spectroscopy. all major 31p resonances observed were assigned to specific compounds. the cells contained inorganic phosphate (1039 nmol/g wet cells), pyrophosphate (16 nmol/g wet cells), nucleoside diphosphates (91 nmol/g wet cells), nucleoside triphosphates (275 nmol/g wet cells), nad(p) (60 nmol/g wet cells), phosphocholine (184 nmol/g wet cells), phosphoethanolamine (214 nmol/g wet cells), cytidine 5'-diphos ... | 1993 | 8319681 |
| interaction between facilitated diffusion of glucose across the plasma membrane and its metabolism in trichomonas vaginalis. | the parasitic protist trichomonas vaginalis transports glucose across the plasma membrane by facilitated diffusion. the km of the transporter for glucose was 1.6 mm. the uptake of labelled glucose in a minimal medium not allowing growth reached saturation only after 2.5 h, indicating the turnover of storage carbohydrate. organisms grown on glucose showed higher activities both of the transporter and of the subsequent metabolic pathway than organisms grown on maltose. at low external glucose conc ... | 1993 | 8319891 |
| effect of recombinant tumor necrosis factor alpha on in vitro amoebicidal activity of murine kupffer cells. | the present study was undertaken to determine whether recombinant tumor necrosis factor alpha (tnf) could induce kupffer cells to kill entamoeba histolytica parasite in vitro. c57bl/6 mice were used in this study. the liver was perfused and kupffer cells harvested and treated with tnf for 6 h. it was found that kupffer cells treated with tnf are able to kill amoebic trophozoites in vitro. these results further show that amoebicidal activity of tnf-activated kupffer cells is dependent on the rati ... | 1993 | 8326852 |
| [carriers and excretors of protozoa]. | causative agents of intestinal infections in humans are about 10 pathogenic or facultative pathogenic species of protozoa of which giardia lamblia, entamoeba histolytica, cryptosporidium parvum and enterozytozoon bieneusi are discussed. g. lamblia from humans is morphologically indistinguishable from giardia isolates originating from several species of domestic and wild mammals. swiss giardia isolates of human, sheep, cattle and dog origin could be transmitted to giardia-free rodents and were ra ... | 1993 | 8333895 |
| electrophoretic karyotype and chromosome assignments for a pathogenic and a nonpathogenic strain of entamoeba histolytica. | the electrophoretic karyotypes of a pathogenic and a nonpathogenic strain of entamoeba histolytica were determined by pulsed-field gel electrophoresis. a number of previously isolated genes were assigned to specific chromosomal bands. significant differences between the chromosomal patterns of these strains as well as in the assignment of most genes were found. | 1993 | 8335392 |
| predictive value of diagnostic tests and prevalence of invasive entamoeba histolytica infection. | 1993 | 8335998 | |
| human immunodeficiency virus and leprosy. | in summary, clinical leprosy does not appear to be more frequent in hiv-positive than in hiv-negative people in areas where both infections are endemic. there is no evidence that the paucibacillary to multibacillary distribution of patients is altered by hiv infection. there are reports that neuritis is more severe in co-infected people, and that reversal reactions (or, at least, new lesions) may be more frequent after therapy; but these reports are either poorly documented or poorly controlled. ... | 1993 | 8341127 |
| pathogenic entamoeba histolytica: cdna cloning of a histone h3 with a divergent primary structure. | entamoeba histolytica has an unusual nuclear structure characterized by a low degree of chromatin condensation and the absence of stainable metaphase chromosomes. although nucleosome-like particles were observed, no information about histones was available so far. in this paper we describe a cdna clone with significant homology to h3 histones that was isolated from a library of pathogenic e. histolytica. the complete cdna encodes a 15-kda polypeptide, which like the histone sequence from volvox ... | 1993 | 8341328 |
| expression of a recombinant entamoeba histolytica antigen in a salmonella typhimurium vaccine strain. | the expression of a major surface antigen of the intestinal protozoal parasite entamoeba histolytica in an attenuated salmonella typhimurium vaccine strain is described. a polymerase chain reaction fragment derived from cdna encoding the serine-rich entamoeba histolytica protein, srehp, was introduced into s. typhimurium chi 3987 (delta cya delta crp delta asd) using a plasmid expression vector (pya292) containing the aspartate semialdehyde (asd) gene. s. typhimurium expressing recombinant srehp ... | 1993 | 8342325 |
| entamoeba histolytica: the ehzc3 cdna clone encodes a zinc-binding protein. | 1993 | 8344401 | |
| improved method for the concentration and purification of faecal cysts of entamoeba histolytica for use as antigen. | human stool samples are the most widely used source of e. histolytica cysts for diagnosis and research. cysts concentrated from faecal samples are contaminated with faecal debris and bacteria and are unsuitable for use as antigens. other gradient purification and sedimentation procedures result in considerable cyst losses. we present an effective and simple purification procedure using varying densities of percoll that yields an adequate cyst concentration from a moderate cyst load in a single s ... | 1993 | 8345546 |
| entamoeba histolytica: antibody response to recent and past invasive events. | sero-epidemiological data from endemic amoebiasis areas are difficult to evaluate because the serology of individuals affected by an active process of entamoeba histolytica tissue invasion is, at present, almost impossible to distinguish from that of individuals who have had an invasive event in the past. the present study compares serum antigenic recognition frequencies among three groups of individuals with different infective conditions: amoebic liver abscess patients; asymptomatic cyst passe ... | 1993 | 8346990 |
| newer stool based diagnostic tests in infectious diseases: possibilities and pitfalls. | 1993 | 8354530 | |
| detection of entamoeba histolytica antigens in stool in amebiasis. | stool microscopy, the conventional method of diagnosing intestinal amebiasis, fails to detect entamoeba histolytica in more than 30-40% of clinically suspected cases. demonstration of parasite products in clinical specimens has been suggested as an alternative. however, the usefulness of demonstrating amebic antigen in the stools of clinical cases needs to be assessed. | 1993 | 8354532 |
| imported opisthorchis viverrini and parasite infections from thai labourers in taiwan. | in order to determine the prevalence of intestinal parasites among labourers from thailand in taiwan, the stool samples from 1364 thai labourers were examined by the formalin-ether concentration method and 18.0% were found to be infected with one or more parasites. the infection rates of hookworm, opisthorchis viverrini, strongyloides stercoralis, enterobius vermicularis, ascaris lumbricoides, hymenolepis nana, capillaria philippinensis, schistosoma japonicum, echinostoma sp., entamoeba coli, gi ... | 1993 | 8354853 |
| correlation between antiamoebic igg and autoreactive anti-igg in amoebic liver abscess cases. | igg isolated and purified from a healthy human serum through sephadex g-200 and protein a cl 4b sepharose chromatography was used for detection of its own antibodies and correlated with the antiamoebic antibody titres in amoebic liver abscess cases. the mean titres with standard deviation of the self reactive antibodies to serum igg both ala cases and healthy controls show a highly significant difference, and antiamoebic antibody titres (igg) are very much correlated with the autoreactive anti i ... | 1993 | 8359844 |
| recognition of entamoeba histolytica in liver abscess cases through their specific antiamoebic antibodies. | 1993 | 8359857 | |
| serum-independent and serum-dependent cytoadherence in the interaction of entamoeba histolytica with mammalian target cells. | entamoeba histolytica kills target cells only on direct contact, suggesting that trophozoite-mediated cytolysis is initiated by the contact between trophozoites and target cells. we have shown that adherence between e. histolytica and target cells (polymorphonuclear granulocytes, erythrocytes, chinese hamster ovary cells, human colon carcinoma cells) was inhibited by specific carbohydrates, and adherence between e. histolytica and polymorphonuclear granulocytes (pmn) was enhanced by preincubatio ... | 1993 | 8359985 |
| some characteristics of hospitalized hiv seropositive patients in myanmar. | twenty-two hospitalized hiv seropositive patients were studied prospectively between july 1991 and january 1992. the majority of the patients were intravenous drug users (ivdus). their age ranged from 20 to 38 years with a male preponderance of 12 to 1. anemia, lymphopenia and thrombocytopenia were observed in 100%, 36% and 41%, respectively. the common pathogens like malaria parasites, mycobacterium tuberculosis, entamoeba histolytica, streptococcus and salmonella were isolated/identified rathe ... | 1993 | 8362294 |
| analysis of the genomic sequence encoding the 29-kda cysteine-rich protein of entamoeba histolytica. | by analyzing cdna and genomic clones coding for the 29-kda cysteine-rich protein of entamoeba histolytica, substantial sequence differences were found to the 5'-end of a previously described full-length cdna coding for the same protein (reed et al., 1992), which was reported to contain an untranslated 5'-sequence of at least 171 nucleotides, unusual for e. histolytica cdnas. we found evidence that the cdna published by reed et al. (1992) represents a hybridclone composed of two unrelated sequenc ... | 1993 | 8367659 |
| diagnosis of pathogenic entamoeba histolytica infection using a stool elisa based on monoclonal antibodies to the galactose-specific adhesin. | monoclonal antibodies (mabs) directed against pathogen-specific epitopes of the galactose adhesin of entamoeba histolytica were used in an elisa to detect antigen from pathogenic e. histolytica. single stool specimens from 74 patients in bangladesh were used. the elisa for pathogenic e. histolytica was positive in all 12 stool specimens with pathogenic amebae subsequently cultured, in no stool specimens with nonpathogenic e. histolytica and in 2 of 40 stools with other or no intestinal parasites ... | 1993 | 8369019 |
| synthesis, antibacterial and antifungal activity of metal (ii) complexes of 2-acetylpyridine thiosemicarbazones. | 2-acetylpyridine-(4-methylthiosemicarbazone), 2-acetylpyridine-(2- methylthiosemicarbazone) and their metal (ii) complexes have been synthesized and characterized by chemical and spectral methods. they were studied for their antibacterial, antifungal and amoebicidal activity in vitro and for their toxicity in vivo. the ligands and their metal (ii) complexes exhibited significant activity against a wide spectrum of microorganisms. the metal (ii) complexes derived from 2-acetylpyridine-(4-methylth ... | 1993 | 8373503 |
| entamoeba histolytica: electrophysiologic and morphologic effects of trophozoite lysates on rabbit colon. | although in acute intestinal amoebiasis the events occurring during the initial interaction of entamoeba histolytica trophozoites with the mucosa of the large intestine probably determine the invasion by the parasites, an appropriate experimental model to study them does not exist. to develop such a model we used rabbit colonic segments (0.28 cm2) mounted in ussing-type chambers. these preparations had electrophysiological properties (potential difference, short-circuit current, and electrical r ... | 1993 | 8375485 |
| entamoeba histolytica proteins modulate the respiratory burst potential by murine macrophages. | reactive oxygen intermediates are important components of macrophage microbicidal mechanisms and pathogenesis of parasitic disease. the purpose of the present study was to investigate the effect of virulent entamoeba histolytica (strain hm1-imss) on respiratory burst potential of macrophages. pretreatment of elicited peritoneal macrophages (epm) with crude soluble amoebic proteins from 1 to 6 hr was found to prime epm for enhanced o2 and h2o2 release in response to phorbol myristate acetate (pma ... | 1993 | 8386134 |
| electron transport components of the parasitic protozoon giardia lamblia. | the energy metabolism of the intestinal parasite, giardia lamblia, involves the iron-sulphur protein, pyruvate:ferredoxin oxidoreductase. cell fractionation studies showed that this enzyme is associated with the membranes. nadh and nadph dehydrogenases were found in both the membrane and cytosolic fractions. epr spectroscopic studies showed the presence of iron-sulphur clusters in the membrane fraction and in the cytosolic fraction, non-sedimentable at 6 x 10(6) g.min. an acidic, soluble protein ... | 1993 | 8391475 |
| entamoeba histolytica cyst passers. clinical profile and spontaneous eradication of infection. | the present study was carried out to examine whether entamoeba histolytica cyst passers suffer from any parasite-related bowel symptoms and to assess the frequency of spontaneous eradication of this infection. the study was carried out in two parts. in part i, stool samples were collected at random from 3536 individuals living in rural communities around delhi. e. histolytica was isolated by the culture technique in 345 (9.7%) subjects. there was no increase in the prevalence rate of bowel sympt ... | 1993 | 8404403 |
| in vitro effect of recombinant interferon gamma in combination with lps on amoebicidal activity of murine kupffer cells. | the present study examines the role of liver macrophages (kupffer cells), of c57bl/6 mice, as effector cells responsible for the killing of entamoeba histolytica trophozoites in vitro. it was shown that unstimulated kupffer cells were inefficient in the killing of e. histolytica trophozoites in vitro. interferon gamma (ifn-gamma) alone was not able to activate kupffer cells to amoebicidal state. however, interferon gamma and lipopolysaccharide (lps) acted synergistically in this phenomenon. it s ... | 1993 | 8406558 |
| in vitro encystation and excystation of entamoeba histolytica trophozoites. | 1993 | 8406604 | |
| detection of sialic acid in trophozoites of entamoeba histolytica (nih:200). | in two successive experiments detection of sialic acid was achieved from trophozoites of axenically grown e. histolytica (nih:200) using silicic acid column chromatography for the separation of sialosylated lipids and asialosylated lipids from total lipids. sialic acid of the sialosylated lipids was detected through thiobarbituric acid assay followed by acid hydrolysis. these findings indicate that presence of sialic acid is not only a character of the cystic stage but also that of trophozoites ... | 1993 | 8406633 |
| isoenzyme pattern of clones of entamoeba histolytica. | the isoenzyme pattern of clones of e. histolytica isolated from symptomatic and asymptomatic patients of amoebiasis were determined by starch gel electrophoresis. ten parent cultures (uncloned), 5 from symptomatic and 5 from asymptomatic patients were isolated and established in robinsons medium as xenic cultures. a total of 100 clones were isolated from parent cultures but only 65 could be established. starch gel electrophoresis was carried out on the uncloned and cloned cultures. the analysis ... | 1993 | 8406646 |
| entamoeba histolytica: changes in the zymodeme of cloned nonpathogenic trophozoites cultured under different conditions. | in this paper we report the occurrence of changes in the migration of certain isoenzymes of a cloned strain (mav-cinvestav) of entamoeba histolytica. this strain was isolated from an asymptomatic carrier and showed an initial nonpathogenic zymodeme i. the transfer of polyxenic trophozoites from robinson's medium (7% serum) to jones' medium (30% serum) provoked changes in isoenzyme expression, resulting in the conversion of zymodeme i to a zymodeme that was similar to the xvii zymodeme except for ... | 1993 | 8415539 |
| biochemical and immunological studies on soluble antigens of entamoeba histolytica. | the soluble antigens of entamoeba histolytica trophozoites were analysed in detail by biochemical and immunochemical methods. the antigen was highly complex and heterogeneous as revealed by sephacryl s-300 column chromatography, which showed four distinct fractions. the molecular mass of fractions fi, fii, fiii and fiv was 660, 170, 65 and 13 kda, respectively. protein was the major constituent in crude soluble antigen (csa) and fractions fi and fii (67%, 80% and 90%, respectively). polysacchari ... | 1993 | 8415541 |
| dose- and time-dependent functional and structural damage to the colon mucosa by entamoeba histolytica trophozoite lysates. | analysis of the initial interaction between entamoeba histolytica trophozoites and the large intestine is impossible in humans and very difficult in experimental animals. to circumvent this obstacle we treated the luminal side of full-thickness rabbit colon segments mounted in ussing-type chambers with trophozoite lysates of the e. histolytica hm1 virulent strain. exposure to lysates for up to 90 min produced dose- and time-dependent effects on the colon, consisting of (a) increased decay rates ... | 1993 | 8415570 |
| role of infectious agents in exacerbations of ulcerative colitis in india. a study of clostridium difficile. | fifty patients with idiopathic ulcerative colitis--25 with acute exacerbation of the disease (group i) and 25 in quiescent phase (group ii)--were studied. none of the patients had a history of recent exposure to antimicrobial drugs or hospitalization. evidence of infection with protozoal and bacterial agents and/or presence of clostridium difficile toxin was demonstrated in eight (32%) patients in group i and one (4%) patient in group ii (group i vs. group ii, p < 0.05; chi 2 test with yate's co ... | 1993 | 8421140 |
| molecular characterization of the cdna coding for translation elongation factor-2 of pathogenic entamoeba histolytica. | to investigate the humoral immune response of patients with amoebic dysentry against entamoeba histolytica, immunoglobulin g (igg)-immunopositive cdna clones from the pathogenic strain sfl-3 were examined. a large part of the igg-positive cdna clones obtained with one serum encoded highly conserved intracellular proteins. a clone was found that was homologous to translation elongation factor-2 (ef-2). sequence analysis of the ef-2 cdna showed 63.6% amino acid sequence identity with the human hom ... | 1993 | 8422275 |
| the ca(2+)-atpase activity of entamoeba histolytica is exposed towards the medium and towards the lumen of intracellular vesicles. | 1993 | 8426610 | |
| application of the polymerase chain reaction to the epidemiology of pathogenic and nonpathogenic entamoeba histolytica. | we used the polymerase chain reaction (pcr) to study the epidemiology of pathogenic and nonpathogenic entamoeba histolytica in a rural community in mexico. formalin-fixed stool samples were used for extraction of dna. the pcr amplifications were performed using two sets of primers that discriminate between pathogenic or non-pathogenic e. histolytica. a total of 201 randomly selected individuals were studied. among them, 25 (12%) were diagnosed to be infected with e. histolytica by microscopy; pc ... | 1993 | 8427389 |
| localization of myosin heavy chain a in the human pathogen entamoeba histolytica. | to recognize myosin ii in trophozoites of the human pathogen entamoeba histolytica, a specific antimyosin polyclonal serum was raised against a fusion protein consisting of a 146-amino-acid fragment of the myosin ii heavy chain a of e. histolytica (mhca) fused with beta-galactosidase. the hybrid protein was encoded by a chimera gene formed by a dna fragment, from the mhca gene, amplified by polymerase chain reaction and fused with the lacz gene of escherichia coli. polymerase chain reaction-ampl ... | 1993 | 8432587 |
| [febrile status in a traveler returning from the tropics with right-sided upper abdominal pain]. | this is a case report of a patient who presented multiple liver abscesses with entamoeba histolytica five weeks after returning from an one-month trip to thailand. this makes clear that a febrile patient returning from the tropics may not only be suspected of malaria, but that we should think about other possible febrile diseases. respecting the incubation period and other clinical symptoms and signs, we are in most of the cases able to reduce the differential diagnosis to only a few possible on ... | 1993 | 8434207 |
| role of cysteine proteinases of entamoeba histolytica on the cytopathogenicity of axenic trophozoites on rat and hamster hepatocytes in vitro. | the role of amebic cysteine proteinase(s) on the in vitro cytotoxic and cytolytic effects of axenic trophozoites of a virulent strain (hm-1) of entamoeba histolytica has been studied using freshly isolated rat and hamster hepatocytes as target cells. the cytotoxic effect was defined as cell killing without loss of cell structure and the cytolytic effect as cell disintegration. incubation experiments of axenic trophozoites with rat or hamster liver cells in the presence and absence of bovine seru ... | 1993 | 8437063 |
| antigenicity, immunogenicity and vaccine efficacy of the galactose-specific adherence protein of entamoeba histolytica. | entamoeba histolytica is an enteric protozoan that causes amoebic colitis and liver abscess. human immunity to e. histolytica is apparently mediated by a serum antibody response and amoebicidal cellular mechanisms. the galactose-specific adherence protein of e. histolytica is a 260 kda glycoprotein which mediates amoebic in vitro adherence to human colonic mucins, epithelium, and inflammatory cells. amoebic lysis of cells is dependent upon binding by this adherence protein. serum igg and salivar ... | 1993 | 8438621 |
| intestinal parasitic infections and urbanization. | about a third of the population in the cities of developing countries live in slums and shanty towns. by the year 2000 it is estimated that this number will grow to 2200 million, and by 2025 about 57% of the population in developing countries will be in urban areas. the prevalence of infections caused by entamoeba histolytica and giardia intestinalis and the prevalence and intensity of ascaris lumbricoides and trichuris trichiura infections may increase among the rural populations who are migrat ... | 1993 | 8440028 |
| sequence of the 5.8s ribosomal gene of pathogenic and non-pathogenic isolates of entamoeba histolytica. | 1993 | 8441643 | |
| determination of the pathogenicity of entamoeba histolytica. | 1993 | 8443325 | |
| molecular cloning of a rho family gene of entamoeba histolytica. | an entamoeba histolytica gene (eh rho1) was cloned that encodes a putative low-molecular-mass gtp-binding protein, most similar to the ras homologue rho. the eh rho1 open reading frame was 208 amino acids long and encoded a 23-kda protein similar to saccharomyces cerevisiae rho1-rho4 and cdc42 and human rhoa, rac1, and g25k gene products. this similarity was greatest at the nh2 terminus of eh rho1 where two gtp-binding sites and a possible effector site were conserved. a cysteine residue at the ... | 1993 | 8459832 |
| cloning of a virulence factor of entamoeba histolytica. pathogenic strains possess a unique cysteine proteinase gene. | cysteine proteinases are hypothesized to be important virulence factors of entamoeba histolytica, the causative agent of amebic dysentery and liver abscesses. the release of a histolytic cysteine proteinase from e. histolytica correlates with the pathogenicity of both axenic strains and recent clinical isolates as determined by clinical history of invasive disease, zymodeme analysis, and cytopathic effect. we now show that pathogenic isolates have a unique cysteine proteinase gene (acp1). two ot ... | 1993 | 8473498 |
| interaction between trophozoites of entamoeba histolytica and the human intestinal cell line ht-29 in the presence or absence of leukocytes. | studies on the interaction between trophozoites of entamoeba histolytica of pathogenic or non-pathogenic origin and epithelial cells of the human intestine can contribute to the understanding of the pathogenesis of invasive amoebiasis. we have examined the interaction of virulent e. histolytica with the human colonic carcinoma cell line ht-29. differentiated ht-29 cells are comparable to the mucosa cells to which e. histolytica attaches physiologically. adherence between e. histolytica trophozoi ... | 1993 | 8475032 |
| expression of sequences related to c-myc in entamoeba. | the evolutionarily conserved proto-oncogene c-myc is involved in both proliferation and differentiation processes of higher eukaryotic cells. we report here the identification and characterization of sequences homologous to c-myc in different entamoeba species using a fragment of the mammalian c-myc gene as a probe. this probe hybridized with fragments of 3.5 and 3.4 kilobases (kb) in e. histolytica hindiii of ecori digested dna. in e. invadens it recognized fragments of 3.1 and 2.8 kb, and in l ... | 1993 | 8475034 |
| phagocytosis and proteinase activity are not related to pathogenicity of e. histolytica. | to examine the relationship between phagocytosis, proteinase activity and pathogenicity of axenically grown trophozoites of e. histolytica strain hm-1:imss four different cultures were used: (1) a culture preserved in our laboratory for over 4 years, which lost its pathogenicity 3 years ago; (2) a culture passaged several times through hamster liver, which lost its pathogenicity recently; (3) a highly virulent culture supplied by another laboratory; and (4) amebas recovered from hamster liver ab ... | 1993 | 8475035 |
| proteinases resistant to sodium dodecyl sulfate-induced autoproteolysis of entamoeba histolytica hk9 trophozoites. | 1993 | 8475037 |