Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| [extraintestinal infections caused by vibrio parahaemolyticus and vibrio alginolyticus at the county of funen 1987-1992]. | over a six-year period we detected 30 clinical infections caused by halophilic vibrios in a restricted geographical area. vibrio parahaemolyticus infections were found in 13 patients (three with wound infections, ten with ear infections), and vibrio alginolyticus infections in 17 patients, all of whom had ear infections. from 1987 to 1990, infections caused by marine vibrios were found in at most four cases annually, in 1992 in six instances, whereas over a five-month period in 1991 we experienc ... | 1994 | 7941065 |
| [the ecological and epidemiological aspects of the spread of vibrio parahaemolyticus in a freshwater region]. | for the first time vibrio parahaemolyticus have been isolated in this country in a freshwater region both from patients with the diarrheal syndrome and from environmental objects. in the region of the lower course of the volga morbidity in acute enteric infections (aei) caused by these vibrios may reach 9.1% of all aei cases in some years. the formation of an ecological niche suitable for the circulation of these bacteria has taken place in the volga delta. all these facts indicate that freshwat ... | 1994 | 7941868 |
| f0f1-atpase of vibrio parahaemolyticus: purification using new detergents and characterization. | previous attempts to isolate a stable f0f1-atpase complex (h(+)-translocating atpase) from vibrio parahaemolyticus have been unsuccessful. using new non-ionic detergents (alkyl thiomaltosides), a stable f0f1 complex with a high specific activity (15-25 units/mg protein) was purified and characterized. the purified f0f1-atpase consists of eight subunits (alpha, beta, gamma, delta, epsilon, a, b and c). the new detergents, in combination with sucrose (or glycerol), lipid, dithiothreitol and phenyl ... | 1994 | 7947906 |
| characterization of a glucose transport system in vibrio parahaemolyticus. | cells of a glucose-pts (phosphoenolpyruvate:carbohydrate phosphotransferase system)-negative mutant of vibrio parahaemolyticus transport d-glucose in the presence of na+. maximum stimulation of d-glucose transport was observed at 40 mm nacl, and na+ could be replaced partially with li+. addition of d-glucose to the cell suspension under anaerobic conditions elicited na+ uptake. thus, we conclude that glucose is transported by a na+/glucose symport mechanism. calculated vmax and km values for the ... | 1994 | 7961512 |
| structure and iron transport activity of vibrioferrin, a new siderophore of vibrio parahaemolyticus. | the structure of vibrioferrin, a siderophore from vibrio parahaemolyticus, was elucidated based on a combination of partial hydrolysis and spectroscopic techniques. hplc of purified vibrioferrin showed two peaks with an area ratio of approximately 2:1. however, upon reinjection of each of those isolated compounds, the original chromatographic pattern was obtained, indicating an equilibrium between two compounds in aqueous solution. consistent with this finding, most of the nmr signals of vibriof ... | 1994 | 7961600 |
| [serotypes of urease producing vibrio parahaemolyticus and their relation to possession of tdh and trh genes]. | we analysed 467 isolates of vibrio parahaemolyticus for possession of tdh/trh gene in comparison with urease production and serotypes. strains possessing tdh+/trh-, tdh+/trh+, tdh-/trh-, and tdh-/trh- show positive urease production 2.1, 100, 65.7, 100%, respectively. serotypes of o1:k69, o3:k6, o3:k72, o6:k18, o6:k46 and o1:kut were frequently positive (100% except 91.7% of the latest one) in urease production. all isolates of o3:k6 prossessed trh, whereas all isolates of certain serotypes incl ... | 1994 | 7963797 |
| properties of the na+/h+ antiporter in vibrio parahaemolyticus. | the properties of the na+/h+ antiporter in vibrio parahaemolyticus, a slightly halophilic bacterium, were investigated using everted membrane vesicles. it appears that at least two na+/h+ antiporters are present, one that is ph-dependent and one that is ph-independent. these two antiporters appear to correspond to the nhaa and nhab antiporters of escherichia coli, respectively. it seems that amiloride strongly inhibits the ph-dependent antiporter. na+ is the best substrate for both of the two v. ... | 1994 | 7982898 |
| moty, a component of the sodium-type flagellar motor. | energy to power the rotation of bacterial flagella can be derived from the proton or sodium transmembrane potential. until now, genes encoding a bacterial sodium-type flagellar motor have not been defined. a gene, moty, encoding one component of the sodium-type flagellar motor of vibrio parahaemolyticus was cloned by complementation of a mot- mutant strain. sequencing revealed an open reading frame of 879 nucleotides in which a transposon conferring a motility defect mapped. overexpression of mo ... | 1994 | 8021208 |
| a mutant toxin of vibrio parahaemolyticus thermostable direct hemolysin which has lost hemolytic activity but retains ability to bind to erythrocytes. | a mutant toxin, r7, of thermostable direct hemolysin (tdh) with a single amino acid substitution at glycine 62 was analyzed. the hemolytic activity of r7 decreased to less than 1/1,000 of that of wild-type tdh, and its mouse lethality was undetectable. this mutant toxin, however, showed a marked inhibitory effect on hemolysis by wild-type tdh. enzyme immunoassay and flow cytometric analysis demonstrated that r7 retained approximately 50% of the ability to bind to erythrocytes compared with that ... | 1994 | 8039901 |
| construction and characterization of an isogenic mutant of vibrio parahaemolyticus having a deletion in the thermostable direct hemolysin-related hemolysin gene (trh) | a mutant, vp-mx02, having a deletion in the gene (trh) for the thermostable direct hemolysin-related hemolysin (trh) was constructed by double-crossover gene conversion from a trh-producing vibrio parahaemolyticus strain, th3996. the deleted region was the upstream half of trh. hemolysis was completely lost, and trh antigen was undetectable in the mutant. administration of the mutant into ligated rabbit small intestines elicited partial, but apparent, fluid accumulation. these results suggest th ... | 1994 | 8045908 |
| demonstration and characterization of simultaneous production of a thermostable direct hemolysin (tdh/i) and a tdh-related hemolysin (trhx) by a clinically isolated vibrio parahaemolyticus strain, th3766. | simultaneous production of a thermostable direct hemolysin (tdh)-like toxin (tdhx) and a tdh-related hemolysin (trh)-like toxin (trhx) by a clinical isolate (strain th3766) of kanagawa phenomenon-positive vibrio parahaemolyticus was demonstrated and characterized. the two hemolysins were differentially purified by column chromatography on hydroxyapatite and immunoaffinity columns. the molecular weight of the two hemolysins were estimated to be 23,000 by sodium dodecyl sulfate-polyacrylamide gel ... | 1994 | 8262624 |
| [the etiological structure of food poisonings caused by marine halophilic vibrios]. | 1994 | 7879513 | |
| antigenicity and antigenic cross-reactivity of outer membrane proteins of vibrio parahaemolyticus. | antigenicity of outer membrane proteins was studied and compared between kanagawa positive (clinical) and negative (environmental) strains of vibrio parahaemolyticus. murine antibodies recognized a wide range of outer membrane proteins of kanagawa negative strains as antigens with molecular masses ranging between 102 kda to 14 kda. however, only a few of the total outer membrane proteins of clinical isolates were antigenic and comprised a 55kda protein as the major antigen. although a marked dif ... | 1994 | 7727894 |
| chitovibrin: a chitin-binding lectin from vibrio parahemolyticus. | a novel 134 kda, calcium-independent chitin-binding lectin, 'chitovibrin', is secreted by the marine bacterium vibrio parahemolyticus, inducible with chitin or chitin-oligomers. chitovibrin shows no apparent enzymatic activity but exhibits a strong affinity for chitin and chito-oligomers > dp9. the protein has an isoelectric ph of 3.6, shows thermal tolerance, binds chitin with an optimum at ph 6 and is active in 0-4 m nacl. chitovibrin appears to be completely different from other reported vibr ... | 1994 | 7696854 |
| epidemiology and spectrum of vibrio diarrheas in the lower cross river basin of nigeria. | in 1991 a cholera epidemic occurred in nigeria. the features of this cholera outbreak in a single hospital in cross river, nigeria, were examined. microbiologic techniques included the use of thiosulphate citrate bile-salts sucrose (tcbs) medium for culture of all stool specimens. vibrio isolates from diarrheic patients included v. cholerae-o1 (75), v. cholerae non-o1 (10) and v. parahaemolyticus (21). the illnesses were diverse, ranging from mild to severe, and in most instances requiring hospi ... | 1994 | 7812244 |
| plasmid-determined degradative metabolism and halophilism of vibrio parahaemolyticus. | a set of 25 kanagawa(+) and kanagawa(-) strains of v. parahaemolyticus was studied for their ability to degrade hydrocarbons in minimal media. all strains gave positive results with respect to crystal violet (cv), methyl violet, liquid paraffin, benzene, naphthalene and phenol. the cv double ring (cvdr) response had earlier appeared to be a significant pathogenic marker [chakrabarti et al, indian j med res, 85 (1987) 508]. the cvdr response was found also to be a biodegradative marker, and corre ... | 1994 | 7814040 |
| [basic and clinical studies of fleroxacin on infectious enteritis. research group of am-833 on infectious enteritis]. | a clinical study was conducted on fleroxacin (flrx) in 143 patients and carriers with infectious enteritis (shigellosis, salmonella enteritis, campylobacter enteritis, pathogenic escherichia coli enteritis, vibrio parahaemolyticus enteritis, cholera, multiple bacterial infections, pathogen-negative enteritis). furthermore, its antibacterial activity against clinical isolates, fecal concentration and effect on fecal microflora were conducted. flrx was administered orally in doses of 200 mg once a ... | 1994 | 7829908 |
| vibrindole a, a metabolite of the marine bacterium, vibrio parahaemolyticus, isolated from the toxic mucus of the boxfish ostracion cubicus. | the etoac extract of the whole culture medium of vibrio parahaemolyticus, which inhabits the toxic mucus of the box fish ostracion cubicus, afforded a new indole-derived natural product, vibrindole a [1], along with some known cyclic dipeptides and indoles. the structure of 1 was determined by analysis of its physicochemical characteristics. | 1994 | 7853008 |
| siderophore-mediated utilization of iron bound to transferrin by vibrio parahaemolyticus. | vibrio parahaemolyticus produces a structurally novel type of siderophore, termed vibrioferrin, in response to iron-limitation. this study was performed to examine whether vibrioferrin can assimilate iron from human iron-binding proteins for growth. comparison of the growth rates between v. parahaemolyticus aq 3354 and its spontaneously arising, vibrioferrin-deficient mutant revealed that vibrioferrin was able to sequester iron from 30% iron-saturated human transferrin for growth, but not from h ... | 1994 | 7854209 |
| seasonal variations in the occurrence of vibrio vulnificus along the dutch coast. | the seasonal variation in the occurrence of v. vulnificus in relation to water temperature and salinity was studied along the dutch coast. in two consecutive years v. vulnificus strains could be isolated in august when the water temperature was highest. the indole-positive strains isolated from north sea water samples were identical to most strains isolated from human disease and from the environment. however, strains isolated from four of five patients living in countries around the north sea w ... | 1994 | 8150002 |
| the origin of non-outbreak vibrio parahaemolyticus infections on guam. | a study of vibrio parahaemolyticus infected patients not associated with known outbreaks and controls matched for sex, ethnicity and age (+/- 5 years) was conducted on guam. cases were asked if they had eaten seafood within the 24-hour period preceding onset of illness and controls were asked if they had eaten seafood within the 24-hour period preceding their interview. cases were more likely than controls to have eaten seafood (or = 37.59, cl [8.30-220.24]). | 1994 | 8165739 |
| hemolymph lectins of the blue crab, callinectes sapidus, recognize selected serotypes of its pathogen vibrio parahaemolyticus. | 1994 | 8192339 | |
| effect of ionizing dose rate on the radioresistance of some food pathogenic bacteria. | food pathogenic bacteria including listeria monocytogenes (1a1 and atcc 19111), staphylococcus aureus (gd13 and atcc 13565), escherichia coli o157:h7 (atcc 35150), salmonella typhimurium, yersinia enterocolitica, vibrio parahaemolyticus, and campylobacter jejuni were exposed to various rates of ionizing radiation (0.78, 2.6, and 22 kgy/h) emitted by three different 60co irradiators. d10 values (d10 is the radiation dose required to eliminate 90% of a bacterial population (one logarithmic cycle r ... | 1994 | 8069779 |
| responses of diverse heterotrophic bacteria to elevated copper concentrations. | the influence of copper on the growth of escherichia coli, pseudomonas aeruginosa, bacillus cereus, bacillus subtilis, vibrio parahaemolyticus, vibrio alginolyticus (three strains), and an unidentified vibrio sp. was examined in batch cultures. the effects of copper at micromolar concentrations varied from undetectable to complete growth inhibition. each strain was able to recover from a growth lag observed after copper addition at a characteristic concentration. copper concentrations that allow ... | 1994 | 8069784 |
| urea hydrolysis can predict the potential pathogenicity of vibrio parahaemolyticus strains isolated in the pacific northwest. | the ability of some strains of vibrio parahaemolyticus to hydrolyze urea (uh+) can be used as a marker to predict which strains isolated from molluscan shellfish harvested in the pacific northwest are potentially pathogenic. the thermostable direct hemolysin-producing (tdh+) characteristic is a marker that is correlated with potential pathogenicity, and all of the tdh+ strains that we have isolated have been found to be uh+. most of the uh+ strains belong to somatic antigen groups o3, o4 and o5. ... | 1994 | 8085837 |
| isolation and sequence of an endochitinase-encoding gene from a cdna library of trichoderma harzianum. | there are no reports of gene sequences coding for extracellular chitinolytic enzymes from filamentous fungi, even though these enzymes are considered critical to the biological control of plant pathogenic fungi. the purpose of this paper was to report the isolation of a gene (then-42) encoding endochitinase (ech) from trichoderma harzianum strain p1, describe its sequence, and to determine whether it was related to genes coding for enzymes with similar functions from prokaryotic or other eukaryo ... | 1994 | 8125293 |
| [a case of cardiogenic shock caused by vibrio parahaemolyticus]. | a case of a 53 year old healthy female complaining of diarrhea and abdominal pain after taking raw fish is presented. she immediately went into shock and unconsciousness. central venous pressure was 8 cmh2o and her ecg showed a first-degree av block and st-t changes in almost all leads. after mechanical ventilation and administration of dopamine, dobutamine, cefotiam, ciprofloxacin, she became alert and recovered from her critical condition. v. parahaemolyticus which produces thermostable direct ... | 1994 | 8138673 |
| bacterial enteric pathogens in uncooked foods in thai markets. | in developing countries, the morbidity and mortality rates of gastrointestinal tract infections from food borne bacteria have been difficult to establish. most studies have only been able to gather data prospectively from isolated geographic sources, rather than from large point-source epidemics. this study investigates the types of bacterial enteric pathogens found in food that was collected in a community in western thailand, where sporadic cases of hemolytic uremia syndrome and cholera have b ... | 1994 | 9815313 |
| identification and characterization of a protease produced by vibrio parahaemolyticus in iron-limited medium. | two proteolytic proteins (about 43 and 90 kda) were produced by clinical strains of vibrio parahaemolyticus cultured in iron-limited medium. the 43 kda-protease was partially purified by ammonium sulfate precipitation, ultrafiltration fractionation and deae-sephacel chromatography. this protease had an optimum ph range of 7 to 8, and an optimum reaction temperature of about 40 degrees c. it was heat-labile, being partially inactivated by heat-treatment at 60 or 90 degrees c for 10 min. the prote ... | 1994 | 9747347 |
| evaluation of virulence factors in environmental isolates of vibrio species. | strains of vibrio parahaemolyticus, vibrio fluvialis and vibrio mimicus isolated from seafood and seawater were examined for characteristics related to infectivity, such as enzymatic activity and animal assays. all strains hydrolysed dna, starch, gelatin and chitin. variable results were obtained with the haemolysin, chondroitin, collagen, elastin and lecithin tests. production of thermostable direct haemolysin by v. parahaemolyticus was detected in 7.1% strains derived from seafood and 2% from ... | 1993 | 8139466 |
| rapid and specific detection of the thermostable direct haemolysin gene in vibrio parahaemolyticus by the polymerase chain reaction. | synthetic oligonucleotide primers derived from a sequence of the thermostable direct haemolysin (tdh) gene were used in a polymerase chain reaction (pcr) amplification technique to detect this gene in strains of vibrio parahaemolyticus. a total of 36 tdh-producing, and 89 tdh-negative vibrio parahaemolyticus strains and 46 other vibrios and enteric pathogens were studied. in all, 36 strains of vibrio parahaemolyticus from which the tdh gene could be successfully amplified by pcr were found to be ... | 1993 | 8126441 |
| vibrios among patients of good socioeconomic conditions during the cholera epidemic in recife, brazil. | between march and july, 1992, we screened for vibrio all fecal samples submitted for bacteriologic diagnosis at a private clinical laboratory in recife. of 1435 cultures examined only 1 (0.07%) was positive for v. cholerae 01, biovar eltor, serovar inaba, but 17 (1.2%) yielded non-cholera vibrio (v. cholerae non-01; v. fluvialis; v. furnissii, v. parahaemolyticus and vibrio spp). thus, v. cholerae 01, differently of other enteropathogenic vibrios, spared individuals of good socioeconomic conditi ... | 1993 | 8115794 |
| respiration-driven na+ pump of the marine vibrio is encoded by chromosomal dna. | a plasmid-cured strain of the marine vibrio alginolyticus 138-2 retains a respiration-driven na+ pump. examinations of several strains of v. alginolyticus and v. parahaemolyticus revealed that these murine vibrio always possessed the respiration-driven na+ pump irrespective of the presence or absence of plasmids. these results strongly suggested that the genes for the na+ pump were encoded by chromosomal dna. | 1993 | 8220320 |
| internalization of oligodeoxyribonucleotides by vibrio parahaemolyticus. | the bacterium vibrio parahaemolyticus was tested for its ability to internalize unmodified as well as modified dna oligomers without attempting to permeabilize the cells. these experiments were conducted to establish whether it may be feasible to employ antisense oligomers for control of gene expression in vibrio species without heat-shocking or electroporating the cells. the bacterium was found to bind radiolabeled synthetic oligodeoxyribonucleotides that were added to culture media. incorporat ... | 1993 | 8155978 |
| [monitoring of halophilic vibrios in sea waters of the northern coasts of the province of rome]. | 1993 | 7524555 | |
| [hygienic and commercial quality of senegalese frozen shrimp]. | 100 samples of shrimps (frozen entire raw shrimps: fers and frozen peeled raw shrimps: fprs) collected in one urban factory, were studied to assess their hygienic and commercial quality. in addition to bacteriological analysis, the dosage of residual bisulphite content was carried out. it appeared that: level of aerobic plate was high for fers: 2.55.10(4)/g and weak for fprs: 2.45.10(5)/g; level of psychrotophic micro organisms was relatively high for fers: 1.07.10(4)/g and very weak for fprs: 0 ... | 1993 | 7882843 |
| septicemia caused by vibrio parahemolyticus: a case report. | vibrio parahemolyticus is a halophilic marine vibrio commonly associated with outbreaks of acute gastroenteritis which also sometimes causes serious wound infection. it is an uncommon cause of septicemia. a few reports suggest that patients with chronic liver disease and leukemia are more susceptible. a case of liver cirrhosis with septicemia caused by this organism is discussed. the patient's condition rapidly deteriorated, and he died 12 hours after admission. | 1993 | 8299034 |
| inhibition of psychrotrophic organisms by propionicin plg-1, a bacteriocin produced by propionibacterium thoenii. | propionibacterium thoenii strain p127, which produces the bacteriocin propionicin plg-1, was grown in a skim milk medium and produced bacteriocin in that medium. no bacteriocin activity was detected in skim milk medium in which strain p127-1, a bacteriocin-negative variant of strain p127, had been grown. five psychrotrophic spoilage or pathogenic organisms (one strain each of listeria monocytogenes, pseudomonas fluorescens, vibrio parahaemolyticus, yersinia enterocolitica, and one strain of cory ... | 1993 | 8326023 |
| introduction of a series of alkyl thiomaltosides, useful new non-ionic detergents, to membrane biochemistry. | we synthesized a series of non-ionic detergents, alkyl thiomaltosides, and investigated their properties and usefulness. we solubilized membrane proteins of vibrio parahaemolyticus using the detergents. with octyl thiomaltoside, nonyl thiomaltoside, decyl thiomaltoside, or undecyl thiomaltoside, we observed satisfactory solubilization of the membrane proteins. alkyl thiomaltosides possessing longer alkyl chains showed better solubilization than ones possessing shorter chains. h(+)-translocating ... | 1993 | 8340350 |
| enterotoxigenic bacteria in the sudden infant death syndrome. | faecal samples from 123 infants who died with sudden infant death syndrome (sids) and from a comparative group of 52 age-matched babies were analysed for toxigenic bacteria and their toxins. serum samples from the sids infants were also analysed for these toxins. a significantly higher proportion of toxigenic bacteria and their toxins were found in faecal samples of sids babies than in samples from the comparative group. these toxins were also found in serum from the sids babies. clostridium per ... | 1993 | 8345506 |
| heat and acid sensitivity of motile aeromonas: a comparison with other food-poisoning bacteria. | the present study was undertaken to compare the heat and acid sensitivity of aeromonads with those of other food-poisoning bacteria. it became obvious that aeromonads were more sensitive to heat than escherichia coli o157:h7, staphylococcus aureus, and salmonella typhimurium. aeromonads were killed in peptone water within 2 min at 55 degrees c, while the other bacteria survived heating at 55 degrees c for more than 15 min. aeromonas cells were also less resistant to heat in hamburger steaks. the ... | 1993 | 8347426 |
| reactive arthritis induced by vibrio parahaemolyticus. | we describe a case of reactive arthritis after vibrio parahaemolyticus infection. v. parahaemolyticus is the principal bacteria involved in the causation of foodborne gastroenterocolitis outbreaks in japan. several bacteria have been recognized as being involved in the development of reactive arthritis and this is the first report of reactive arthritis induced by v. parahaemolyticus. | 1993 | 8350314 |
| identification of oligosaccharides consisting of d-glucuronic acid and l-glycero-d-manno- and d-glycero-d-manno-heptose isolated from vibrio parahaemolyticus o2 lipopolysaccharide. | 1993 | 8370031 | |
| [incidence of bacterial contamination in the ares-betanzos estuary (nw spain)]. | the presence of bacterial indicators of fecal pollution and v. parahaemolyticus in the estuary of ares-betanzos (ría de ares-betanzos, nw of spain) was investigated. resistance patterns of coliform bacteria to eight antibacterial agents were also determined. in general, high numbers of indicator bacteria were found; for instance, heterotrophic bacteria ranged between 1.82 x 10(2) to 1.9 x 10(4) cfu/ml and up to 4.6 x 10(3)/100 ml fecal coliforms in surface waters and 1.2 x 10(4)/100 ml fecal str ... | 1993 | 8397962 |
| cation flux studies of the lesion induced in human erythrocyte membranes by the thermostable direct hemolysin of vibrio parahaemolyticus. | vibrio parahaemolyticus, an important agent of seafood-borne gastroenteritis, expresses several putative virulence factors that could account for the disease symptoms of infected humans, namely, diarrhea, nausea, and abdominal cramps. the pathogenicity of v. parahaemolyticus correlates well with the kanagawa phenomenon (the hemolytic ability of strains grown on wagatsuma blood agar), implicating the thermostable direct hemolysin (tdh) as the predominant toxin responsible for pathogenicity. tdh-i ... | 1993 | 8406820 |
| treatment of acute bacterial diarrhea: a multicenter international trial comparing placebo with fleroxacin given as a single dose or once daily for 3 days. | this study was designed to test the efficacy of 400 mg fleroxacin given orally as a single dose or once daily for 3 days against acute bacterial diarrhea. a group of 508 adults with acute diarrhea were entered into a randomized, double-blind, placebo-controlled, multicenter trial. patients were examined and asked about numbers of liquid stools daily for 3 days and at 5 days after start of treatment. repeat stool samples were obtained for culture on days 3 and 5 after start of treatment. a total ... | 1993 | 8452179 |
| ciprofloxacin and loperamide in the treatment of bacillary dysentery. | to compare the safety and efficacy of loperamide plus ciprofloxacin with those of ciprofloxacin alone in the treatment of bacillary dysentery. | 1993 | 8452323 |
| identification of genes encoding components of the swarmer cell flagellar motor and propeller and a sigma factor controlling differentiation of vibrio parahaemolyticus. | vibrio parahaemolyticus possesses two distinct motility systems, the polar system used for swimming in liquid environments and the lateral system used for swarming over surfaces. growth on surfaces induces swarmer cell differentiation and expression of the lateral motility system. mutants, created by transposon mutagenesis of a clone expressing lateral flagellin and gene disruption in v. parahaemolyticus, were unable to swarm and failed to make lateral flagellin; therefore, unlike the case for t ... | 1993 | 8501040 |
| release of the outer membrane vesicles from vibrio cholerae and vibrio parahaemolyticus. | we found numerous small vesicles released from the cell by thin sectioning of the plate culture of vibrio cholerae and v. parahaemolyticus fixed with the freeze-substitution technique. from the broth media of exponentially growing bacteria we could collect the vesicles by the centrifugation but not enough without fixation. the vesicles are encompassed with a membrane structure similar to the outer membrane of these bacteria. the anti-o (inaba) serum reacted with the surface of the vesicles and t ... | 1993 | 8502178 |
| vibrio parahaemolyticus septicemia associated with water-skiing. | 1993 | 8507240 | |
| vibrio parahaemolyticus has a homolog of the vibrio cholerae toxrs operon that mediates environmentally induced regulation of the thermostable direct hemolysin gene. | in an effort to identify the regulatory gene controlling the expression of the tdh gene, encoding the thermostable direct hemolysin of vibrio parahaemolyticus, we examined total dna of aq3815 (a kanagawa phenomenon-positive strain) for sequences homologous to that of the toxr gene of vibrio cholerae. the extracted dna gave a weak hybridization signal under reduced-stringency conditions with a toxr-specific dna probe. cloning and sequence analysis of the probe-positive sequence revealed an operon ... | 1993 | 8509337 |
| luminol-dependent chemiluminescence of hemocytes derived from marine and estuarine molluscs. | hemocytes of two marine molluscs, nerita albicilla (gastropod) and mytilus edulis (bivalve), were stimulated in vitro with zymosan and live cells of vibrio parahaemolyticus and escherichia coli as determined by luminol-dependent chemiluminescence (cl). the cl response was enhanced in the presence of the respective molluscan plasma. hemocytes of an estuarine gastropod, clithon retropictus, showed low cl response to zymosan and v. parahaemolyticus, which was slightly enhanced in the presence of c. ... | 1993 | 8513011 |
| [incidence of vibrio parahaemolyticus in raw fish, oysters, and shrimp]. | the incidence of vibrio parahaemolyticus in fresh seafood sold in guadalajara, was studied by two procedures. these two procedures were compared to choose a reliable technique when outbreaks of v. parahaemolyticus illness occur. for one year, 57 samples of fresh oysters, fish and shrimp were analyzed for mesophilic aerobic bacteria (mab) content, v. parahaemolyticus and ph. total volatile nitrogen (tvn) was also determined in samples of fish and shrimp. mab were counted by the pour plate method, ... | 1993 | 8047729 |
| extraintestinal infections caused by vibrio parahaemolyticus and vibrio alginolyticus in a danish county, 1987-1992. | over a 6-year period we detected 30 clinical infections caused by halophilic vibrios in a restricted geographical area. vibrio parahaemolyticus infections were found in 13 patients (3 with wound infections, 10 with ear infections), and vibrio alginolyticus infections in 17 patients, all of whom had ear infections. from 1987 to 1990, infections caused by marine vibrios were found in at most 4 cases annually, in 1992 in 6 instances, whereas in a 5-month period in 1991 we experienced 15 cases of ex ... | 1993 | 8052813 |
| lipopolysaccharide isolated from a new o-antigenic form (o13) of vibrio parahaemolyticus. | the chemical properties of a lipopolysaccharide (lps) isolated from a new o-antigenic form (o13) of vibrio parahaemolyticus were investigated. the lps contained glucose, galactose, l-glycero-d-manno-heptose and glucosamine. 2-keto-3-deoxy-octonate (kdo) was not detected in the lps by the periodate-thiobarbituric acid test (weissbach's reaction) under conventional hydrolysis conditions. instead, phosphorylated kdo (x1 and x2) was found in its strong-acid hydrolysate. this sugar composition was id ... | 1993 | 7684809 |
| a chemotaxonomic study of vibrio fluvialis based on the sugar composition of the polysaccharide portion of the lipopolysaccharides. | a chemotaxonomic study was carried out with a new serotyping scheme comprising 35 o-antigen groups of vibrio fluvialis on the basis of the sugar composition of the polysaccharide portion of their lipopolysaccharide (lps). a previously developed rapid method of preparing samples for compositional sugar analysis was employed. the 35 o-antigen groups were divided into 21 chemotypes. it is noted that a rarely occurring component sugar of gram-negative bacterial lps, d-glycero-d-manno-heptose, and tw ... | 1993 | 7684810 |
| [experimental study of hyperostosis induced by hypervitaminosis a]. | radiological and histological studies were made on retinoid-induced hyperostosis in rats. vitamin a (va) was administered intraperitoneally in rats for 6 months. hyperostosis was observed in 94 percent of rats administered va and in 38 percent of the control. chondrocytes and vascular proliferation were observed in the attachment of the tendons and in the anterior corner of the vertebral body after 3 months. hyperostosis was observed as osteophytes in the attachment of ligaments or tendons and a ... | 1992 | 1280297 |
| isolated severe thrombocytopenia and bleeding caused by piperacillin. | bleeding and severe thrombocytopenia developed in a 71-year-old man who had been receiving piperacillin 5 g intravenously every 8 h for 9 days for the treatment of pseudomonas aeruginosa septicemia. after piperacillin was discontinued, the platelet counts became normal. rechallenge was made with 2 g of piperacillin intravenously resulting in thrombocytopenia within 6 h of piperacillin administration. the platelet count normalized after 3 days when no further piperacillin was given. | 1992 | 1287819 |
| [bacteriological study of traveller's diarrhoea. 4) isolation of enteropathogenic bacteria from patients with traveller's diarrhoea at osaka airport quarantine station during 1984-1991]. | during the last 8 years (1984 to 1991), 16,639,233 overseas travellers were quarantined at osaka airport quarantine station and 38,326 travellers reported that they were (or had been) suffering from diarrhoea. bacteriological examination of stools from 12,573 persons revealed the following results. 1) various enteropathogenic bacteria were isolated from 3,669 cases (29.2%) examined. the predominant species of bacteria isolated were as follows: salmonella, 1049 cases; plesiomonas shigelloides, 10 ... | 1992 | 1293222 |
| [the lysogeny of vibrio parahaemolyticus of serovar o4:k12]. | lysogeny has been first established in strains of parahemolytic vibrios of serovar o4:k12. moderate phages belonged to morphological group iv by home a. s. tikhonenko's classification and were presented by one serological type. no correlation has been revealed between sensitivity to moderate phages of parahemolytic vibrios and specificity of "o"- or "k"-serotypes. | 1992 | 1293486 |
| features of cholera and vibrio parahaemolyticus diarrhoea endemicity in calabar, nigeria. | the clinical and epidemiological features of acute vibrio diarrhoeal disease were studied in 881 patients seen at the university of calabar teaching hospital (ucth), calabar, nigeria, between january and december 1989. stools and rectal swabs of patients and randomly-selected control subjects were microscopically and culturally examined for the presence of enteric pathogens. households of vibrio diarrhoea cases and matched controls were visited for ecologic studies. of a total of 108 (12.3%) cul ... | 1992 | 1294392 |
| a survey of urease-positive vibrio parahaemolyticus strains isolated from traveller's diarrhea, sea water and imported frozen sea foods. | the frequency of urease-positive vibrio parahaemolyticus among isolates from patients, imported frozen sea foods and the environment (sea water) was studied. the highest isolation frequency of urease-positive v. parahaemolyticus was found in clinical isolates (11.2% out of 204 strains examined). urease-positive v. parahaemolyticus was found in 5.7% of 88 frozen sea food-isolates examined, but no strains isolated from sea water were urease-positive. the isolates were further examined for the prod ... | 1992 | 1294393 |
| purification and characterization of monomeric isocitrate dehydrogenase with nadp(+)-specificity from vibrio parahaemolyticus y-4. | nadp(+)-dependent isocitrate dehydrogenase [idh: ec 1.1.1.42] was purified to electrophoretic homogeneity from vibrio parahaemolyticus y-4, and shown to be a monomeric protein of molecular weight 80,000 with a pi of 5.0. the amino acid composition and partial sequence at the n-terminus resembled those reported for other bacterial monomeric idhs. immunotitration with antisera to the monomeric and dimeric enzymes (antisera to idh-ii and -i of vibrio abe-1) showed an immunochemical distinction betw ... | 1992 | 1295895 |
| effects of phosphate in the production of hemolysin in vibrio parahaemolyticus. | vibrio parahaemolyticus is known to produce several types of hemolysin, the most document is the tdh and trh. a new type of hemolysin whose expression is repressed by the presence of phosphate in the medium is reported. | 1992 | 1298639 |
| microbiological aspects and technological need: technological needs for nitrates and nitrites. | nitrate and nitrite are used in meat and fish curing, and in the manufacture of certain cheeses. nitrate itself has little antimicrobial effect and in most applications could be replaced by lower concentrations of nitrite. further, improved hygiene diminishes the need for nitrite. the antimicrobial activity and technological needs for nitrate and nitrite are reviewed. it is concluded that the technological needs for nitrite in meat products stored at < 10 degrees c could be met by added nitrite ... | 1992 | 1298641 |
| [vestibular evoked potentials. diagnostic trends]. | the contribution of short-latency evoked potentials to the exploration of auditory, visual and somatosensory neurosensory pathways is capital. the determination of short-latency vestibular evoked potentials has encountered many difficulties, mainly due to the necessity to find a specific, very short stimulation in order to select and synchronize the vestibular nerve fibers. recent studies in animals have demonstrated short-latency potentials evoked by angular accelerations, linear accelerations ... | 1992 | 1304102 |
| expression and release of neuroregulators during development: monoamines and neuropeptides. | the turnover of catecholamines (ca) was determined in the adrenal medulla and brain of rat fetuses and pups. in general we found a considerable increase soon after birth. the expression of mrna for ca-synthesizing enzymes was also considerably enhanced in the adrenals shortly after birth. furthermore, we demonstrated increased expression of neuropeptides after birth, increased synthesis of mrna encoding for neuropeptide y in the adrenals 24 h after birth; and considerable activation of the subst ... | 1992 | 1306802 |
| brazilian urease-positive strains of vibrio parahaemolyticus carry genetic potential to produce the tdh-related hemolysin. | 1992 | 1308550 | |
| polar and lateral flagellar motors of marine vibrio are driven by different ion-motive forces. | various species of marine vibrio produce two distinct types of flagella, each adapted for a different type of motility. a single, sheathed polar flagellum is suited for swimming in liquid medium, and numerous unsheathed lateral flagella, which are produced only under viscous conditions, are suited for swarming over viscous surfaces. both types of flagella are driven by reversible motors embedded in the cytoplasmic membrane. here we report that the energy source for the polar flagellar motor of v ... | 1992 | 1309599 |
| kappa-opioid receptor stimulation abolishes mu- but not delta-mediated inhibitory control of spinal met-enkephalin release. | the possible opioid control through delta, mu and kappa receptors of the spinal release of met-enkephalin-like material (melm) was investigated in halothane-anaesthetized rats. the intrathecal perfusion of the delta agonist dtlet (10 microm) or the mu agonist dago (10 microm) resulted in a marked inhibition of melm release, which could be prevented by the selective antagonists naltrindole and naloxone, respectively. although the kappa agonist u 50488 h (10 microm) was inactive per se, it complet ... | 1992 | 1317026 |
| catabolite repression of the h(+)-translocating atpase in vibrio parahaemolyticus. | cells of vibrio parahaemolyticus grown in the presence of glucose showed reduced (by about 40%) oxidative phosphorylation. with this observation as a basis, we examined the effect of glucose on the level of h(+)-translocating atpase. the addition of glucose to the growth medium reduced the specific activity and the amount of the h(+)-translocating atpase in membrane vesicles of v. parahaemolyticus. these reductions were reversed by adding cyclic amp (camp) to the growth medium. we cloned some pa ... | 1992 | 1328162 |
| fimbriation, capsulation, and iron-scavenging systems of klebsiella strains associated with human urinary tract infection. | thirty-two strains of klebsiella pneumoniae and seven strains of klebsiella oxytoca isolated from urinary tract infections in elderly adults were analyzed for capsular antigens, iron-scavenging systems, and fimbriation. all strains were capsulated. twenty-seven different k antigens were identified among the strains, with no particular antigen dominating. all strains produced the iron-scavenging system enterochelin as analyzed by bioassay and dna hybridization. in contrast, the aerobactin iron-se ... | 1992 | 1347287 |
| [h2-receptor antagonist-refractory ulcer--its pathophysiology and role of proton-pump inhibitors]. | among h2-receptor antagonist (h2ra)-refractory ulcers, non-responders that did not heal after 5 months therapy had high intraluminal ph in the basal condition and high sensitivity to inhibition of acid secretion by h2ra but possessed gastric mucosa to generate less prostaglandins. combination therapy of pge1-analogue with h2ra healed these ulcers by 60%. proton-pump inhibitor (ppi) exerted a complete inhibition of acid secretion in these patients and the rate of healing was 88%. helicobacter pyl ... | 1992 | 1347325 |
| the role of pili in colonization of the rabbit intestine by vibrio parahaemolyticus na2. | vibrio parahaemolyticus na2 and its pili were studied in relation to intestinal colonization. the isolated pili were adhesive to the intestinal epithelium. the adhesion of the organisms was blocked by masking the epithelial receptor with the purified pili, or by treating the organisms with anti-pilus antibody (fab fraction). these results suggest that the pili of v. parahaemolyticus na2 play an important role in the adhesion of the organisms to the rabbit intestine. | 1992 | 1350048 |
| termination of second messenger signaling in olfaction. | by using isolated rat olfactory cilia and a fast kinetics methodology, it has been demonstrated that odorant-induced second messenger signaling in the millisecond time range is terminated via phosphorylation reactions catalyzed by specific protein kinases. the cyclic adenosine nucleotide pathway is turned off by kinase a activity, whereas the inositol trisphosphate cascade is terminated by kinase c. the data support the concept that desensitization of odorant responses involves phosphorylation o ... | 1992 | 1370581 |
| towards a phylogeny of the genus vibrio based on 16s rrna sequences. | the inter- and intrageneric relationships of the genus vibrio were investigated by performing a comparative analysis of the 16s rrnas of 10 species, including four pathogenic representatives. the results of immunological and 5s rrna studies were confirmed in that the genus is a neighboring taxon of the family enterobacteriaceae. with regard to the intrageneric structure, vibrio alginolyticus, vibrio campbellii, vibrio natriegens, vibrio harveyi, vibrio proteolyticus, vibrio parahaemolyticus, and ... | 1992 | 1371064 |
| electrophoretic and chemical characterization of lipopolysaccharides of vibrio parahaemolyticus. | lipopolysaccharides (lpss) isolated from three kanagawa-positive and three negative strains of vibrio parahaemolyticus were characterized by using electrophoretic, immunochemical, and chemical methods. the results of this study indicated that the lpss of all six strains of v. parahaemolyticus examined did not have an o-specific side chain. these v. parahaemolyticus lpss appeared to have molecular weights similar to that of the rough-type (ra) lps of salmonella typhimurium tv-119 and might just c ... | 1992 | 1374376 |
| lateral flagella of vibrios: serological classification and genetical similarity. | lateral (l-) flagella-having vibrios were classified into 13 h-serogroups (flagellar antigen serogroups) by means of h-agglutination test. vibrio parahaemolyticus was classified into 3 serogroups, hl1 to 3. v. alginolyticus and v. harveyi were classified into 5 and 3 serogroups, respectively, but 2 of those were serogroups common to the both species. v. fluvialis and v. furnissii constituted a same serogroup, hl8. cross-reactivity between each serogroup was not observed in h-agglutination test, ... | 1992 | 1376395 |
| disparate effects of calcium channel blockers on pressure dependence of renin secretion and flow in the isolated perfused rat kidney. | using the model of isolated perfused rat kidneys this study was performed to investigate whether or not voltage-operated calcium channels are essentially involved in the pressure control of renin secretion from the kidneys. at a perfusion pressure of 100 mm hg (13.3 kpa) renin secretory rates were 4.2 +/- 0.7 (ng angiotensin i h-1) min-1 g-1. stepwise reduction of renal perfusion pressure to 80, 60, and 40 mm hg (10.6, 8.0, 5.3 kpa) resulted in an increase of renin release yielding a 30-fold sti ... | 1992 | 1382264 |
| isolation and partial characterization of a compound with siderophore activity from vibrio parahaemolyticus. | a compound with siderophore activity was purified by successive column and thin layer chromatographic procedures from dowex 1 x 8 extracts of culture supernatants of vibrio parahaemolyticus aq 3354. the strain synthesized the compound in culture media containing less than 2 microm added fecl3. hydrolysis of the compound yielded alanine, ethanolamine, citric acid and 2-ketoglutaric acid. the 1h-nmr spectrum exhibited the presence of a residue from each of these components in the intact molecule. ... | 1992 | 1387860 |
| influence of dopaminergic and serotonergic neurons on intravenous ethanol self-administration in the rat. | rats implanted with chronic indwelling intravenous catheters and allowed access to a self-administration apparatus learned to self-inject intravenous ethanol. ethanol concentrations of 0.5, 1.0, and 2.0%, corresponding to a dose/injection of 1, 2, and 4 mg/kg, respectively, were consistently self-injected. self-injection was not acquired or maintained with ethanol doses of 0.5 or 8 mg/kg/injection. saline replacement of ethanol reservoirs led to marked increases in lever-pressing response in ani ... | 1992 | 1388276 |
| urease production in vibrio parahaemolyticus: a potential marker for virulence. | 1992 | 1397235 | |
| incidence of toxigenic vibrios in foods available in taiwan. | a total of 1088 vibrios and related species were isolated from seafood and aquacultured foods available in taiwan. they were identified as vibrio alginolyticus, v. cholerae, v. fluvialis i, v. fluvialis ii, v. parahaemolyticus, v. mimicus, aeromonas caviae, a. hydrophila, a. sobria and other species. incidence of these vibrio and aeromonas species in these foods was high. vibrio parahaemolyticus was frequently found in seawater and in foods of freshwater origin. the vibrio isolates were examined ... | 1992 | 1399913 |
| vibrio-associated gastroenteritis in the lower cross-river basin of nigeria. | a total of 120 vibrio species were isolated from 588 patients with acute diarrheal disease during an outbreak of gastrointestinal tract infections at different locations in the lower cross river basin of nigeria. vibrio cholerae o1, biotype el tor, serotype ogawa, was the prominent organism isolated from the vibrio-associated diarrheal cases. during the 3 months of study, v. cholerae non-o1 was recovered from 10 patients while vibrio parahaemolyticus was recovered from 19 patients. the significa ... | 1992 | 1400976 |
| [in vitro antimicrobial activity of dr-3355, a new quinolone antibacterial agent, against clinical isolates of enteritis-causing bacteria]. | we determined the minimum inhibitory concentration (mic) of dr-3355, a newly developed quinolone-derivative antibacterial agent, against clinical isolates of various bacterial species from enteritis patients, and compared them with those of ofloxacin (oflx), ciprofloxacin (cpfx), nalidixic acid (na), ampicillin (abpc), kanamycin (km). mic90 of dr-3355 against 94 strains of shigella spp. and 5 strains of escherichia coli, 36 strains of salmonella spp., 22 strains of vibrio cholerae, 5 strains of ... | 1992 | 1402063 |
| [methods to detect the thermostable direct hemolysin gene and a related hemolysin gene of vibrio parahaemolyticus by pcr]. | 1992 | 1404920 | |
| characterization of a vibrio cholerae virulence factor homologous to the family of tonb-dependent proteins. | irga is an iron-regulated virulence factor for infection in an animal model with classical vibrio cholerae strain 0395. we detected gene sequences hybridizing to irga at high stringency in clinical isolates in addition to 0395, including another classical strain of v. cholerae, three v. cholerae strains of the el tor biotype, three non-o1 isolates of v. cholerae, and individual isolates of vibrio parahaemolyticus, vibrio fluvialis, and vibrio alginolyticus. no hybridization to irga was seen with ... | 1992 | 1406279 |
| chemotactic activity of hemocytes derived from a brackish-water clam, corbicula japonica, to vibrio parahaemolyticus and escherichia coli strains. | hemocytes from adult and juvenile specimens of a brackish-water clam, corbicula japonica, were attracted chemotactically to live cells of vibrio parahaemolyticus and escherichia coli strains in a balanced salt solution, which was enhanced significantly in the presence of respective c. japonica plasma. chemotactic attractions of adult's and juvenile's hemocytes were seen also in artificial seawater at a similar level to those in the balanced salt solution. chemotactic attractions of juvenile's he ... | 1992 | 1420564 |
| vibrio parahaemolyticus septicemia in a patient with neutropenic leukemia. | 1992 | 1420695 | |
| the regulation of microsomal glucose-6-phosphatase differs between liver and kidney. | 1992 | 1426558 | |
| activation of t cells in vivo using anti-cd3 and staphylococcal enterotoxins. | one of the major problems in clinical immunity is that neoplastic and virally infected cells are insufficiently immunogenic to trigger an immune response. during the past several years, our laboratory has explored the use of t-cell-specific reagents including monoclonal antibodies (mabs) and staphylococcal enterotoxins to amplify immune responses. this report summarizes our efforts to augment immunity with these agents. | 1992 | 1428401 |
| thermostable, salt tolerant, wide ph range novel chitobiase from vibrio parahemolyticus: isolation, characterization, molecular cloning, and expression. | a chitobiase gene from vibrio parahemolyticus was cloned into plasmid puc18 in escherichia coli strain dh5 alpha. the plasmid construct, pc120, contained a 6.4 kb vibrio dna insert. the recombinant gene expressed chitobiase [ec 3.2.1.30] activity similar to that found in the native vibrio. the enzyme was purified by ion exchange, hydroxylapatite and gel permeation chromatographies, and exhibited an apparent molecular weight of 80 kda on sds-polyacrylamide gel electrophoresis. chitobiose and 6 mo ... | 1992 | 1429506 |
| modification of the disc assay by the incorporation of monoclonal antibody staining. | the use of immunohistochemical staining with the disc assay is described. this modified staining procedure allows differentiation of viable human tumor cells from normal cells in biopsy preparations, facilitating the evaluation of slides from chemosensitivity assays. samples from all and cll patients were processed using both the disc and modified moab-disc procedure. the predictive results were compared and found to be identical. this procedure is advantageous in determining the chemosensitivit ... | 1992 | 1434746 |
| use of an oligonucleotide probe to detect vibrio parahaemolyticus in artificially contaminated oysters. | a 26-mer oligonucleotide specific to vibrio parahaemolyticus was synthesized from a 1,275-bp thermostable direct hemolysin (tdh) gene. this oligonucleotide probe specifically reacted with dna from 89 of 95 v. parahaemolyticus isolates but not with dna from other vibrios or other enteric and nonenteric organisms (n = 48). the probe hybridized with southern blots of 0.5-kb hindiii-restricted chromosomal dna fragments from all but five v. parahaemolyticus test isolates. the probe could be used to d ... | 1992 | 1444377 |
| [the comparative characteristics of vibrio cholerae hemolysins]. | biochemical and biological properties of in vivo and in vitro hemolysins of cholera germs isolated by different authors as well as hemolysins of vibrio parahaemolyticus and escherichia coli have been comparatively characterized. according to the above data hemolysin of cholera germ el tor is a thermolabile protein with molecular mass of 60-80 thou.; it is cytotoxic, enteropathogenic and lyses all the species of erythrocytes. hemolysins of v. eltor and germs are immunologically related. hemolysin ... | 1992 | 1453988 |
| identification of amino acid changes affecting yeast uroporphyrinogen decarboxylase activity by sequence analysis of hem12 mutant alleles. | the molecular basis of the uroporphyrinogen decarboxylase defect in eleven yeast 'uroporphyric' mutants was investigated. uroporphyrinogen decarboxylase, an enzyme of the haem-biosynthetic pathway, catalyses the decarboxylation of uroporphyrinogen to coproporphyrinogen and is encoded by the hem12 gene in the yeast saccharomyces cerevisiae. the mutations were identified by sequencing the mutant hem12 alleles amplified in vitro from genomic dna extracted from the mutant strains. four mutations lea ... | 1992 | 1471989 |
| transforming growth factor beta and basic fibroblast growth factor synergistically promote early bovine embryo development during the fourth cell cycle. | developmentally competent bovine blastocysts were produced by adding transforming growth factor beta (tgf beta) and basic fibroblast growth factor (bfgf) to serum-free cultures of in vitro produced, 2-cell bovine embryos. the effects of tgf beta were evaluated because this growth factor signals synthesis and secretion of the extracellular matrix component fibronectin and its receptor. previous investigations have demonstrated that fibronectin promotes early bovine embryo development in vitro. th ... | 1992 | 1472373 |
| purification and characterization of kanagawa haemolysin from vibrio parahaemolyticus. | the haemolysin of a kanagawa-phenomenon-positive vibrio parahaemolyticus strain was purified to apparent homogeneity by acid precipitation, deae-trisacryl, hydroxyapatite and fplc (mono-q) columns: 1.4 micrograms of protein gave a single band on conventional sds-page with silver staining. the haemolysin was not inactivated by heating for 10 min at 100 degrees c. it was a monomeric protein with a molecular weight estimated to be 29 kda by page under denaturing and non-denaturing conditions. the h ... | 1992 | 1475518 |
| the thermostable direct hemolysin of vibrio parahaemolyticus is a pore-forming toxin. | the hemolytic mechanism of thermostable direct hemolysin (tdh), a possible virulence factor of vibrio parahaemolyticus, was studied. we demonstrated that tdh acts as a "pore-forming toxin" in temperature-dependent and -independent steps. the first temperature-dependent step requires only about 1-2 min incubation at 37 degrees c and makes a "pore" with a functional diameter of approximately 2 nm. the pore size was deduced from the molecular diameter of the colloidal inhibitory polysaccharides. th ... | 1992 | 1477791 |
| ultrastructural alterations of brain cortex in rat following intraperitoneal administration of mercuric chloride. | intraperitoneal administration of a single dose (6 mg/kg body weight) of mercuric chloride (hgcl2) results in ultrastructural changes in brain cortex of the rats. 18 hours after administration of hgcl2 the accumulation of dense deposits of mercury in nerve and glial cell cytoplasm was observed. the quantity of microglia in neuropil was also increased in this experimental group. we postulate that these cerebral macrophages can play an important role in the process of intoxication, too. 5 days aft ... | 1992 | 1479187 |