Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| bacterial aetiology of infantile diarrhoea in papua new guinea. | bacterial examination of stools of children with diarrhoea was carried out at port moresby, papua new guinea, where little information is available concerning the causative bacteria of diarrhoea. shigellae, enterotoxigenic escherichia coli, bacillus cereus, staphylococcus aureus, campylobacter jejuni, and aeromonas hydrophila were isolated with the decreasing frequency in that order. among these, enterotoxigenic e. coli and c. jejuni were isolated for the first time in this country. although vib ... | 1990 | 2362308 |
| aeromonas, acclimation, and penicillin as complications when leeches are applied to skin flaps in rabbits. | the medicinal leech, hirudo medicinalis, has been recognized by plastic surgeons for its ability to reduce congestion and improve blood flow in grafted skin flaps by withdrawing excess blood. the leech's ability to digest blood is due to the presence of aeromonas hydrophila in the gut of the leech. in this report we describe the occurrence of aeromonas hydrophila septicaemia in rabbits used in a study to evaluate the efficacy of the medicinal leech on abdominal skin flap survival. in new zealand ... | 1990 | 2366512 |
| [aeromonas species as cause of diarrhea and infections outside the gastrointestinal tract in the netherlands]. | in a national survey in the period may 1986-december 1987, aeromonas was isolated from 277 out of 16,857 (1.6%) samples of watery, bloody or mucous stool, from patients with diarrhoea. there was a clear seasonal pattern (less than 1% in winter, up to 3% in summer). a. caviae was isolated most frequently (49%), followed by a. sobria (35%) and a. hydrophila (15%). some non-identifiable strains were isolated as well. aeromonas were isolated in particular from faeces of patients aged over 70 years ( ... | 1990 | 2366898 |
| aeromonas hydrophila as cause of hemorrhagic septicemia in a ground-hornbill (bucorvus abyssinicus). | an acute hemorrhagic septicemia in a captive ground-hornbill (bucorvus abyssinicus) is reported. aeromonas hydrophila was isolated from lungs, liver, kidney, and intestines. postmortem lesions were characterized by hemorrhages in the internal organs. microscopically, necrotic lesions were found in the liver, lungs, and kidney. factors that might have contributed to the clinical disease condition are discussed. | 1990 | 2369389 |
| lymphomyeloid cells, susceptibility to erythrodermatitis of carp and bacterial antigens. | pronounced changes occur in the kidneys carp injected with extracellular product (ecp), lipopolysaccharide (lps) of a. salmonicida subsp. salmonicida, freund's complete adjuvant (fca). ecp caused 50-70% decrease in macrophages and neutrophils, whereas blasts and lymphocyte-like cells increased fca-elicited increased percentages of blasts and a left shift of neutrophils after lps injection. after both ecp and fca injections, the density of major leucocyte types and red blood cells (rbc) in the ki ... | 1990 | 2369967 |
| differences in the phagocytosis of four bacteria by channel catfish neutrophils. | the purpose of this study was to compare the phagocytosis of aeromonas hydrophila, edwardsiella ictaluri, edwardsiella tarda and micrococcus luteus by channel catfish neutrophils. various aspects of opsonization effect and bactericidal ability of channel catfish neutrophils were investigated. percent phagocytosis ranged from a low of 1% to a high of 91%. the highest percent phagocytosis and phagocytic indices routinely occurred with normal serum and were highest for m. luteus (91.78%, 55.25) and ... | 1990 | 2369968 |
| aeromonas hydrophila diarrhea in a long-term care setting. | infectious diarrheal diseases can be a serious problem in long-term care institutions. aeromonas hydrophila, a facultative, anaerobic gram-negative rod, has not been implicated previously in an acute diarrheal outbreak in this setting. we report 17 patients with this disorder all characterized by the acute onset of painless, nonbloody diarrhea. thirteen of 17 (76%) patients had two to four loose bowel movements and a duration of illness of less than 48 hours. eleven of 17 (65%) patients were afe ... | 1990 | 2370399 |
| effect of transport medium on recovery of aeromonas species in intestinal infections. | 1990 | 2373092 | |
| phenotypic study of bacteria associated with the caribbean sclerosponge, ceratoporella nicholsoni. | heterotrophic bacteria associated with the caribbean sclerosponge, ceratoporella nicholsoni (hickson), were found to occur extracellularly and were confined to the mesohyl regions of the sponge tissue. physiological, metabolic, and morphological attributes of the culturable bacteria associated with the sponge were recorded by using numerical taxonomy methods for the analysis of 158 phenotypic attributes. morphometric methods were used to determine the proportion of the total sponge-associated ba ... | 1990 | 2383012 |
| [aeromonas hydrophila in the drinking water in djibouti: commensal germ or diarrhea-causing agent?]. | to investigate the bacteriological quality of drinking water used by inhabitants of the republic of djibouti who were not supplied with piped running water, we analysed 16 fresh-water samples from various sources. only 3 samples were sterile; they were taken from village pumps and from a water-truck. eleven samples yielded colonies of aeromonas hydrophila too numerous to be counted; they were taken from water tanks, metal barrels, or wells dug in either dry river beds or along the seashore. we s ... | 1990 | 2385169 |
| in vitro susceptibility of aeromonas caviae, aeromonas hydrophila and aeromonas sobria to fifteen antibacterial agents. | in vitro testing of the activity of 15 antibacterial agents against 522 clinical isolates of aeromonas species demonstrated some species-associated trends. amoxicillin plus clavulanic acid was effective against approximately 45% of aeromonas caviae and aeromonas hydrophila, but all aeromonas sobria isolates were resistant. aztreonam, piperacillin and mezlocillin were highly active against all the strains of aeromonas tested. ticarcillin was equally effective against aeromonas caviae and aeromona ... | 1990 | 2387294 |
| dynamics of aeromonas hydrophila, aeromonas sobria, and aeromonas caviae in a sewage treatment pond. | the spatiotemporal dynamics of aeromonas spp. and fecal coliforms in the sewage treatment ponds of an urban wastewater center were studied after 20 months of sampling from five stations in these ponds. isolation and identification of 247 aeromonas strains were undertaken over four seasons at the inflow and outflow of this pond system. the hemolytic activity of these strains was determined. the aeromonas spp. and the fecal coliform distributions showed seasonal cycles, the amplitude of which incr ... | 1990 | 2389929 |
| classification of the spoilage flora of fish, with special reference to shewanella putrefaciens. | one hundred and fifty-nine gram-negative strains isolated from refrigerated fish, taken from the baltic sea or swedish inland waters, together with 32 reference strains of shewanella, pseudomonas, aeromonas and alcaligenes, were phenotypically classified using 124 unit characters. data were processed by the simple matching (ssm) and jaccard (sj) coefficients, and unweighted pair group algorithm with arithmetic averages. fourteen clusters were defined at the 75% sj similarity level which correspo ... | 1990 | 2391295 |
| a method for detecting shiga toxin and shiga-like toxin-i in pure and mixed culture. | shiga toxin and shiga-like toxins (slts, syn. verotoxins) are currently detected by tissue culture assays that are expensive, time-consuming and require specialised facilities and experienced personnel. we have developed a rapid method to detect shiga toxin and slt-i (verotoxin 1) based on their binding to globotriosyl ceramide (gb3). bound toxin was then detected by an enzyme-linked immunosorbent assay (elisa) with monoclonal antibodies. the direct detection of cytotoxins from pure culture plat ... | 1990 | 2391709 |
| distribution and characterization of hemolytic, and enteropathogenic motile aeromonas in aquatic environment. | a year-long survey on the distribution of motile aeromonas species in the surface waters of riverine and marine environments was conducted. the filtered membranes were directly placed onto the modified pril-xylose-ampicillin agar for the enumeration of aeromonas species. high counts of motile aromonads were found in riverine stations and this bacterial population was also observed in significant quantities in polluted marine samples. in the identification of 2,444 isolates, three species of moti ... | 1990 | 2392066 |
| [effect of the isolation medium and isolation conditions on the isolation of the bacterial species spectrum in drinking water]. | with the method of colony-count estimation according to german standards (trinkwasserverordnung, tvo), bacteria of the genera aeromonas, shewanella, pseudomonas etc. and (if present) representatives of the family enterobacteriaceae can be detected. longer incubation and application of low nutrient media enhance the colony counts up to 100-fold, and other bacteria can be isolated, in most cases belonging to the genera pseudomonas, alcaligenes, acinetobacter, flavobacterium etc.. for the detection ... | 1990 | 2393490 |
| antibiotic combination effect on the clinical isolates of aeromonas hydrophila. | the effect of antibiotic combinations on 191 clinical strains of aeromonas hydrophila was analysed by checkerboard microdilution methods. the combinations of antibiotics were as follows: amikacin (an) + carbenicillin (cb), an + cephalexin (clx), an + piperacillin (pip), an + cefoperazone (cfp), an + ceftriaxone (roc), gentamicin (gm) + cb, gm + clx, gm + pip, gm + cfp, gm + roc, gm + tetracycline (tc). the results of this study indicated that (i) the effect of an + cb and an + pip was considered ... | 1990 | 2394181 |
| [clinical aeromonas hydrophila contain the dna fragments with nucleotide sequence similar to those of hemolysin and cholera-toxin gene]. | the artificially synthesized aquatic aeromonas hydrophila partial aerolysin gene dna (ae, containing 48 oligonucleotides), and partial cholera-toxin gene dna fragments (c1 and c2, containing 34 and 19 oligonucleotide, respectively) were used as probes to determine their dna sequence homology to the chromosome dna of 191 strains of the clinically isolated a. hydrophila, using the colony hybridization test. the three probes had a positive reaction with clinical a. hydrophila strains. several posit ... | 1990 | 2394187 |
| [cloning and sequencing of hemolysin gene from clinical aeromonas hydrophila]. | aerolysin is one of the important virulence factors for aeromonas hydrophila infection. to understand the characteristics of the aerolysin gene in clinical a. hydrophila, a genomic library was constructed by using puc19 as cloning vector. the positive clone containing the aerolysin gene was selected by the colony hybridization method, using the aerolysin probe (an 48 mer oligonucleotide) of aquatic a. hydrophila. four positive out of 1,200 transformants were obtained from the primarily screened ... | 1990 | 2394188 |
| glycerophospholipid:cholesterol acyltransferase complexed with lipopolysaccharide (lps) is a major lethal exotoxin and cytolysin of aeromonas salmonicida: lps stabilizes and enhances toxicity of the enzyme. | an extracellular lethal toxin produced by aeromonas salmonicida was purified by fast-protein liquid ion-exchange chromatography. the toxin is composed of glycerophospholipid:cholesterol acyltransferase (gcat) (molecular mass, 25 kilodaltons) aggregated with lipopolysaccharide (lps), the gcat/lps complex having a molecular mass of about 2,000 kilodaltons, estimated by gel filtration chromatography. the toxin is lethal for atlantic salmon (salmo salar l.) at a concentration of 0.045 micrograms of ... | 1990 | 2394687 |
| cytotoxin and enterotoxin production as factors delineating enteropathogenicity of aeromonas caviae. | twenty-one stool and environmental aeromonas caviae isolates were studied for cytopathogenicity for cultured hep-2 cells. cytotoxic activity was demonstrated by the 21 a. caviae strains and by a. caviae atcc 15468 after challenging hep-2 cells with filtrates from a 24-h-old broth shake culture composed of double-strength, glucose-free trypticase soy broth, incubated at 35 degrees c. heat-stable cytotoxicity was observed 5 h after the addition of 1/5 and 1/10 concentrations of filtrates and consi ... | 1990 | 2394803 |
| transient intestinal colonization by multiple phenotypes of aeromonas species during the first week of life. | the intestinal colonization rate of aeromonas spp. was determined for 52 cesarean-born peruvian neonates. rectal swabs were obtained daily from newborns during their postdelivery hospitalization (mean = 5.5 days), and the gross appearances of their feces (blind determinations) were recorded. aeromonas spp. were recovered from rectal swabs of 12 of 52 (23.1%) infants during their first week of life; the isolates were obtained from 5 of 9 (55.6%) infants with at least one stool with a watery consi ... | 1990 | 2394805 |
| aquatically acquired aeromonas hydrophila wound infection. a report of 3 cases. | the occurrence of aeromonas hydrophila wound infections in healthy hosts after water-associated injury is being reported more frequently. this paper reports our experience with 3 such cases and outlines the importance of recognising the association between a water-related injury and this organism. | 1990 | 2396158 |
| growth characteristics of motile aeromonas spp. isolated from different environments. | the growth of 80 strains of motile aeromonas spp. derived from environments with temperatures above 25 degrees c and below 15 degrees c, respectively, were examined at five temperatures (5 degrees c, 10 degrees c, 25 degrees c, 37 degrees c and 44 degrees c) and four salt levels (0.05%, 2%, 4% and 6% nacl). sixty-one strains were further examined at two ph levels (ph 7.3 and ph 5.3). all strains grew at 25 degrees c and 10 degrees c with the majority of the isolates proliferating from approx. 10 ... | 1990 | 2397155 |
| the bacteriology of fresh and spoiling lake victorian nile perch (lates niloticus). | a total of 177 bacterial cultures isolated from lake victorian nile perch (lates niloticus) were investigated. the flora on newly caught nile perch consisted of organisms belonging to the genera moraxella, alcaligenes, acinetobacter, pseudomonas, aeromonas, micrococcus and other gram-positive organisms. 39% were identified as gram-positive species and 61% were negative in the gram-reaction. three cultures out of 53 investigated caused weak rotten off-odours in sterile fish broth and one culture, ... | 1990 | 2397157 |
| distribution and characteristics of aeromonas in food and drinking water in denmark. | a total of 970 danish commercial foods and drinking water samples were examined for the presence of motile aeromonas spp. with a detection limit of 10(2)/g the frequent prevalence in raw foods was confirmed. aeromonas occurred in 7% of 779 samples of prepared foods; most frequently in whipped cream from ice cream parlors (28%) and mayonnaise salads (10%) with numbers occasionally exceeding 10(5)/g. the prevalence in drinking water was 28% with a detection limit of 1/100 ml. a hydrophila was the ... | 1990 | 2397158 |
| aeromonas species in human septicaemia & diarrhoea. | an increase was observed in the isolation of aeromonas sp. from clinical specimens, especially faeces, from patients with diarrhoeal disease and blood cultures from patients with suspected septicaemias. the isolation rate from diarrhoeal patients was 0.2 per cent in 1978-79 and this increased to 5.0 per cent in 1986-87. it is noteworthy that 13 patients of septicaemia due to aeromonas species were encountered, predominantly in adults with lowered resistance due to underlying disease states inclu ... | 1990 | 2397936 |
| characterization of 22 vibrio species by gas chromatography analysis of their cellular fatty acids. | the cellular fatty acid compositions of 51 vibrio strains belonging to 22 species as well as five aeromonas strains were determined by using capillary gas-liquid chromatography (glc). the major fatty acids were most often hexadecenoic, hexadecanoic and octadecenoic acids. heptadecenoic acid was present in significant amounts in v. alginolyticus, v. natriegens, v. parahaemolyticus and "vibrio navarrensis". twenty fatty acids including branched and hydroxy acids were detected in the genus vibrio. ... | 1990 | 2399366 |
| type i and iv collagen and fibrinogen binding to aeromonas species isolated from various infections. | collagen binding is a common property of strains of aeromonas species. however, agglutination of latex beads coated with types i and iv collagen and fibrinogen with aeromonas cells varied among strains of aeromonas species and their source of isolation. culture media and growth conditions greatly influenced expression of aeromonas cell surface receptors to bind collagen (types i and iv) and fibrinogen immobilized on the latex particles as suggested by the particle agglutination assay (paa). aero ... | 1990 | 2400535 |
| [resistance to antimicrobial agents, hemolytic activity and plasmids in aeromonas species]. | a total of 174 aeromonas isolates consisting of 100 strains from patients with diarrhea being mainly overseas travellers nd healthy subjects, and 74 strains from environmental sources including foods, fish, fresh water, sea water and river soil collected in the area of tokyo metropolis and kanagawa prefecture was examined for the antimicrobial resistance, presence of plasmids and hemolytic activity. almost all the isolates (99.4%) were resistant to aminobenzyl penicillin. the isolation frequency ... | 1990 | 2401817 |
| epidemiology of travelers' diarrhea and relative importance of various pathogens. | each year 12 million persons travel from an industrialized country to a developing country in the tropics or subtropics. these travelers experience a high rate of diarrhea caused by a wide variety of enteric pathogens acquired by ingestion of contaminated food or water. one or more pathogens can be found in the stool of a majority of ill individuals. enterotoxigenic escherichia coli generally are the most frequently identified pathogens, having been found in a median of 42% of travelers' diarrhe ... | 1990 | 2406860 |
| action pattern of aeromonas hydrophila chitinase on partially n-acetylated chitosan. | oligosaccharides from the digestion of 34% n-acetylated chitosan by aeromonas hydrophila chitinase were separated by cm-sephadex c-25 column chromatography. sugar compositions and the sequences of main oligosaccharides were identified through their n-acetylation, their cleavage with exo-glycosidases, and their degradation with nitrous acid. hetero-chitooligosaccharides such as glcn.glcnac, glcn.glcnac.glcnac, glcnac.glcn.glcnac, and glcnac.glcn.glcnac.glcnac, together with glcnac and (glcnac)2, ... | 1990 | 1368549 |
| a general method to generate dna probes for microorganisms. | we present a method that permits the rapid generation of dna probes for eubacteria. in the procedure the variable regions for the 16s rrna genes are amplified using polymerase chain reaction (pcr) technology and primers based on the conserved regions of these genes. following sequencing of the variable regions, a choice is possible for a probe specific for that organism. no knowledge of the molecular biology of the microorganism is required prior to the application of this approach. the generali ... | 1990 | 1369980 |
| pili of aeromonas hydrophila: purification, characterization, and biological role. | aeromonas hydrophila (ae6) has 2 morphologically distinctive kinds of pili. one appeared rigid, channeled, and straight with a diameter of 9 nm (ae6-r pili). the other looked flexible, wavy, and having helical structure with a diameter of 7 nm (ae6-w pili). ae6-r pili were purified and characterized. the pili consisted of a subunit protein with a molecular weight of 18 kda as estimated by sds-page, and contained 42.3% hydrophobic amino acids and one cysteine residue. the pilus was solubilized to ... | 1990 | 1971705 |
| the pili of aeromonas hydrophila: identification of an environmentally regulated "mini pilin". | ultrastructural studies of aeromonas hydrophila strain ah26 revealed two distinctive pilus types: "straight" pili appear as brittle, rod-like filaments, whereas "flexible" pili are supple and curvilinear. straight pili are produced constitutively under all tested conditions of growth. in contrast, the expression of flexible pili is regulated by physical and chemical variables, being produced at 22 vs. 37 degrees c, in a liquid vs. a solid medium, and when the availability of free-iron is reduced ... | 1990 | 1974915 |
| effect of bacterial pyrogen on three lizard species. | 1. three lizard species (callopistes maculatus, gerrhosaurus major, and varanus exanthematicus) were tested for their response to intraperitoneal injection of alcohol-killed aeromonas sobria. 2. a paired experimental design, in which each animal received an injection of sterile saline and 1 x 10(10) a. sobria, was utilized. 3. c. maculatus demonstrated a statistically significant increase in mean selected body temperature (msbt) after bacteria injection. 4. g. major and v. exanthematicus did not ... | 1990 | 1976479 |
| [a membrane-bound alanine aminopeptidase from acinetobacter calcoaceticus. 3. inhibition of the enzyme]. | the alanine aminopeptidase from acinetobacter calcoaceticus is inhibited by sh-reagents like p-hydroxymercuribenzote, ellman's reagent, n-bromosuccinimide, and metal chelating agents like 1,10-phenanthroline. the aap is competitively inhibited by l-amino acids such as leucine, phenylalanine, and valine having hydrophobic side chains. bacitracin (ki = 2.0.10(-6) mol/l) inhibits aap stronger than puromycin (ki = 8.0.10(-6) mol/l). in contrast, the aeromonas aminopeptidase (ec 3.4.11.10) is stronge ... | 1990 | 1980190 |
| pili of an aeromonas hydrophila strain as a possible colonization factor. | aeromonas hydrophila ae6 had 2 morphologically distinctive kinds of pili. one appeared rigid and straight with a diameter of 9 nm (r-pili). the other appeared wavy and flexible with a diameter of 7 nm (w-pili). w-pili were very few on the cell as compared with r-pili. in this study, w-pili were purified and characterized. the pili consisted of a subunit protein with a molecular weight of 21 kda as estimated by sds-page. there was no immunological cross-reaction between w-pili and other cellular ... | 1990 | 1982552 |
| simple, rapid method for direct isolation of nucleic acids from aquatic environments. | direct isolation of nucleic acids from the environment may be useful in several respects, including the estimation of total biomass, detection of specific organisms and genes, estimations of species diversity, and cloning applications. we have developed a method that facilitates the concentration of microorganisms from aquatic samples and the extraction of their nucleic acids. natural water samples of 350 to greater than 1,000 ml are concentrated on a single cylindrical filter membrane (type svg ... | 1989 | 2467621 |
| typing of aeromonas species by protein fingerprinting: comparison of radiolabelling and silver staining for visualising proteins. | a method for typing aeromonas species by silver staining of total soluble proteins separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis is described. there was good agreement with the results obtained by autoradiography of whole-cell proteins for isolates examined by both methods. | 1989 | 2470906 |
| typing of aeromonas strains by dna restriction endonuclease analysis and polyacrylamide gel electrophoresis of cell envelopes. | the outer membrane protein (omp) composition (omp typing) of 46 fecal aeromonas strains from hybridization groups (hgs) 1 (a. hydrophila; n = 10), 4 (a. caviae; n = 16), and 8 (a. veronii; n = 20) were examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis as a phenotypic typing method. almost every isolate of hg-1 and hg-8 had a unique omp profile, in contrast to isolates of hg-4, which were separated into five different omp types. it was possible to recognize hgs 1, 4, and 8 by ... | 1989 | 2473993 |
| bondage, dominance, irrigation, and aeromonas hydrophila: california dreamin'. | 1989 | 2911156 | |
| aeromonas hydrophila colitis presenting as medically refractory inflammatory bowel disease. | 1989 | 2912037 | |
| phenotypic characterization and dna relatedness in human fecal isolates of aeromonas spp. | phenotypic characteristics were used to identify 189 aeromonas strains isolated from human feces. one hundred forty-two of these strains were placed in 11 dna hybridization groups, and the genetic and phenotypic data were compared. according to the criteria of popoff, 66% of the strains were identified as aeromonas caviae, 18% were identified as a. sobria, and 16% were identified as a. hydrophila. some biochemical characteristics differed from the criteria of popoff; 19 of 40 (48%) of tested str ... | 1989 | 2913025 |
| three-dimensional structure of an open form of the surface layer from the fish pathogen aeromonas salmonicida. | cell-free culture supernatants of a lipopolysaccharide (lps) o-polysaccharide-deficient, single-insertion transposon mutant of the tetragonal surface protein array (s layer)-containing fish pathogen aeromonas salmonicida were examined by electron microscopy. negative staining showed that the s layer was released as sheets of tetragonal material, indicating that although surface retention of assembled s layer requires the presence of wild-type lps oligosaccharides, initial assembly of s-layer sub ... | 1989 | 2914846 |
| growth of aeromonas spp. on butzler campylobacter selective agar and evaluation of the agar for the primary isolation of aeromonas spp. from clinical specimens. | the fortuitous finding that aeromonas spp. grew well on butzler campylobacter selective medium (bcsa) in a microaerobic atmosphere at 42 degrees c prompted us to evaluate the performance of bcsa for selective isolation of aeromonads in comparison with ampicillin (30 micrograms/ml) sheep blood agar (asba30). overall recovery rates of aeromonads from 563 stool samples from patients with acute diarrhea were higher on asba30 (70.4%) than on bcsa (56.3%); however, 21 (29.5%) grew only on bcsa. the th ... | 1989 | 2915030 |
| isolation of aeromonas hydrophila from a captive caracal lynx (felis caracal). | aeromonas hydrophila was isolated from the internal organs of a captive caracal lynx (felis caracal) which died of acute septicemia. grossly, patchy areas of focal necrosis were found in the lungs, liver and kidney; there was ulceration in the stomach and intestines. microscopically, lesions contained cellular debris, neutrophils, lymphocytes and gram-negative bacilli. this is the first report of isolation of aeromonas hydrophila from a captive wild animal in nigeria. | 1989 | 2915393 |
| synthesis of glycoconjugates derived from various lipopolysaccharides of the vibrionaceae family. | conjugation of simple ketoses (such as 3-deoxy-d-manno-2-octulosonic acid and n-acetylneuraminic acid) and of various o-specific polysaccharides (from aeromonas hydrophila and aeromonas salmonicida) to the bifunctional spacer 1,6-hexanediamine, was achieved by reductive amination. the saccharide--1-(6-amino)-hexane alkyamines obtained were converted into the corresponding isothiocyanate derivatives and coupled to the free epsilon-amino group of lysine residues of the protein carrier bovine serum ... | 1989 | 2920731 |
| purification and chemical characterization of a cholera toxin-cross-reactive cytolytic enterotoxin produced by a human isolate of aeromonas hydrophila. | a bacterial protein toxin possessing hemolytic, enterotoxic, and cytotoxic activities as well as cross-reactivity to cholera toxin was purified from culture filtrates of a human diarrheal isolate of aeromonas hydrophila (ssu). this cytolytic enterotoxin was purified by ammonium sulfate precipitation, hydrophobic chromatography using phenyl-sepharose, anion-exchange chromatography on deae-bio-gel a, and size-exclusion high-performance liquid chromatography. the factor was a single polypeptide wit ... | 1989 | 2925244 |
| bioactivity and immunological characterization of a cholera toxin-cross-reactive cytolytic enterotoxin from aeromonas hydrophila. | a cytolytic enterotoxin of molecular weight 52,000 was isolated and purified from culture supernatants of a human diarrheal isolate (ssu) of aeromonas hydrophila. the toxin reacted with cholera antitoxin when tested in an enzyme-linked immunosorbent assay and by western blot (immunoblot) analysis. the appearance of cytotoxic and hemolytic activities in culture supernatant occurred simultaneously 8 h after the initial inoculation of the culture. loss of hemolytic activity and cholera toxin cross- ... | 1989 | 2925245 |
| segmental colitis associated with aeromonas hydrophila. | we describe a 40-yr-old woman who presented with abdominal pain and diarrhea, and who was found to have segmental colitis involving the cecum and ascending colon. aeromonas hydrophila was isolated from the stool, and her symptoms responded to appropriate antibiotic therapy. follow-up colonoscopy confirmed complete endoscopic and histologic resolution of her colitis. this report further supports the belief that aeromonas species are pathogens in healthy humans. this organism needs to be considere ... | 1989 | 2929569 |
| the suicide phenomenon in motile aeromonads. | certain strains of motile aeromonas species, including all those of aeromonas caviae examined, were shown to be suicidal. when they were grown in the presence of glucose at both 30 and 37 degrees c, there was rapid die-off of the organisms after 12 h of incubation, and viable cells generally could not be recovered after 24 h. it was shown that this phenomenon was due to the production of relatively high levels of acetic acid by these strains, even during growth under highly aerobic conditions, a ... | 1989 | 2930167 |
| [aeromonas hydrophila. infection in xenopus laevis]. | aeromonas hydrophila caused severe disease in a group of 50 xenopus leavis three weeks after being transferred from their laboratory conditions. the first clinical signs observed were marked pallor, petechiae, lethargy, anorexia, and edema. the duration of the disease was about 48 days, during which 4 animals acquired the disease and 10 died. the study of the bacteriology of the skin and skeletal muscles showed at necropsy subcutaneous edema, hemorrhage, and ascitis. aeromonas hydrophila was iso ... | 1989 | 2486216 |
| aeromonas hydrophila infection complicating digital replantation and revascularization. | two cases of thumb replantation and one of finger revascularization complicated by aeromonas hydrophila infection are reported. two digits were lost because of infection in soft tissue and osteomyelitis. one thumb had extensive necrosis. in all cases the infection was difficult to eradicate, probably because of ischemia. all three patients sustained their injuries while cutting meat or fish. these infection sources have not been previously reported. note is made of aeromonas hydrophila in medici ... | 1989 | 2490149 |
| [yersinia and infantile gastroenteritis]. | fecal samples and intestinal mucosal biopsies of 16 patients in paediatric age, with protracted diarrhoea with blood and mucus, were examined for intestinal pathogens including yersinia. yersinia enterocolitica 1/5/xz and yersinia intermedia 1/18/xz were isolated from fecal samples of two patients. in another child, yersinia enterocolitica, 1/5/xz was isolated from fecal sample, while yersinia enterocolitica 1/self-agglutinable/xz from colon biopsy. the other intestinal pathogens were absent; ae ... | 1989 | 2490392 |
| [aeromonas bacteremia]. | 1989 | 2490659 | |
| [spontaneous peritonitis caused by aeromonas hydrophila]. | 1989 | 2490661 | |
| [pleural empyema caused by aeromonas hydrophila]. | 1989 | 2490662 | |
| [endocarditis caused by aeromonas hydrophila]. | 1989 | 2490663 | |
| [acute salpingitis caused by aeromonas hydrophila]. | 1989 | 2490664 | |
| [aeromonas]. | 1989 | 2490668 | |
| [intestinal infections caused by aeromonas]. | we have identified 38 instances of intestinal infection by aeromonas and 4 by plesiomonas in 16,184 coprocultures carried out during a 4 year period in the hcu of zaragoza. the low prevalence can be related with the fact that specific media for the recovery of these organisms were not used. the species aeromonas sobria (47.36%) and a. caviae (39.47%) were more common than a. hydrophila (13.15%). aeromonas were isolated from patients with and without intestinal disease. the occurrence of gastroin ... | 1989 | 2490673 |
| [application of the heiberg biotypes in the diagnosis of species in the mesophilic aeromonas group]. | 1989 | 2490869 | |
| [bacterial etiologies of acute infantile diarrhea in dakar]. | systematic research into all the bacterial etiological agents responsible for acute diarrhoea was undertaken on 276 diarrhoea stools collected from children aged 0-5 years. particular attention was given to finding bacterial agents usually overlooked during routine checks: campylobacter, vibrionaceae and yersinia enterocolitica. | 1989 | 2491376 |
| in-vitro activity of lomefloxacin in comparison with pefloxacin and ofloxacin. | the in-vitro activity of lomefloxacin (sc 47111, ny-198) was investigated by the determination of mics in agar and in broth, of mbcs in broth, of killing curves and of the duration of the post-antibiotic effect. mics measured in broth and in agar were almost identical. lomefloxacin was two- to eight-fold less active against gram-positive bacteria than ofloxacin. its activity against staphylococcus aureus was independent of resistance to penicillin and oxacillin. the activity of lomefloxacin, ofl ... | 1989 | 2496071 |
| antimicrobial susceptibility of sixty human fecal isolates of aeromonas species. | the mics of 21 antimicrobial agents were determined for 60 strains of aeromonas spp. isolated from human feces. all isolates tested were susceptible to aztreonam, tetracycline, imipenem, moxalactam, pipemidic acid, gentamicin, trimethoprim-sulfamethoxazole, pefloxacin and ciprofloxacin. resistance to erythromycin and streptomycin was observed in all 60 strains. aeromonas caviae was less susceptible to cefamandole, cefotaxime, norfloxacin, chloramphenicol, tetracycline, sulfamethoxazole and trime ... | 1989 | 2496995 |
| isolation and characterization of monoclonal antibodies against alkaline phosphatase of pseudomonas aeruginosa. | monoclonal antibodies against the alkaline phosphatase of pseudomonas aeruginosa were produced from spleen cells of balb/c mice primed with purified alkaline phosphatase of p. aeruginosa atcc 10145 and sp20/ag-14 myeloma cells. the eight stable clones established produced antibodies that reacted by enzyme-linked immunosorbent and indirect immunofluorescence assays with all bacterial strains of p. aeruginosa, including the 17 serotypes and two nontypable strains. three of the clones cross-reacted ... | 1989 | 2501343 |
| aeromonas: the forgotten pathogen. | 1989 | 2504945 | |
| lomefloxacin (sc 47111 or ny-198), a new difluorinated quinolone: comparison of the in vitro activity with other broad spectrum antimicrobials against enterobacteriaceae, acinetobacter spp, aeromonas spp, and pseudomonas aeruginosa. | using a broth microdilution method, we compared the in vitro activity of lomefloxacin to other broad spectrum antimicrobials against clinical strains of enterobacteriaceae, acinetobacter spp, aeromonas spp, and p. aeruginosa. against the enterobacteriaceae and a. hydrophila, lomefloxacin showed excellent activity with mic50 values ranging from 0.12-0.5 micrograms/ml and mic90 values ranging from 0.12-1.0 micrograms/ml. by comparison, lomefloxacin had superior activity to nalidixic acid and diflo ... | 1989 | 2507219 |
| complexing of copper in drinking water samples to enhance recovery of aeromonas and other bacteria. | the presence of copper in drinking water samples at concentrations as low as 10 micrograms/l resulted in a rapid die-off of aeromonads. coliform bacteria, heterotrophic plate count bacteria and faecal streptococci were also sensitive to copper but to a lesser degree than aeromonads. the effect was particularly noticeable in soft water (less than 3 meq/l ca + mg) and at ph-values below 8.0. the toxic effect of copper concentrations up to 500 micrograms/l could be neutralized for a period of up to ... | 1989 | 2512276 |
| influence of modified-atmosphere storage on the growth of uninjured and heat-injured aeromonas hydrophila. | the growth of uninjured and heat-injured aeromonas hydrophila incubated at 5 degrees c (22 days) and 30 degrees c (31 h) under air, n2, and co2 was investigated. at 30 degrees c, the growth patterns of cells on brain heart infusion agar incubated under air and n2 were similar, although slight differences in the lengths of the lag phases and the final populations were detected. the lag phases of cells incubated under air and n2 were substantially longer at 5 degrees c than at 30 degrees c. the po ... | 1989 | 2516434 |
| evolutionary relationships among bacterial carbamoyltransferases. | an immunological approach was used for the study of ornithine carbamoyltransferase (otcase) evolution in bacteria. antisera were prepared against the anabolic and catabolic otcases of pseudomonas aeruginosa and aeromonas formicans as well as against otcase and putrescine carbamoyltransferases from streptococcus faecalis; these antisera were then tested against the unpurified otcases, either anabolic or catabolic, of 34 bacterial strains. extensive cross-reactions were observed between the antise ... | 1989 | 2516870 |
| purification and characterization of aeromonas hydrophila beta-hemolysin. | aeromonas hydrophila hemolysin was excreted in our culture conditions during the stationary growth phase. the toxin was purified to homogeneity by a three-step method: ultrafiltration, acid precipitation in the presence of rna and anion exchange chromatography with fplc apparatus. beta-hemolysin is a protein not associated with lipids, carbohydrates or nucleic acids whose subunit mol. wt is 51,000. the mol. wt determined by polyacrylamide gel electrophoresis suggests that the molecule is in a tr ... | 1989 | 2516922 |
| [bacterial isolation in infants hospitalized for acute diarrhea]. | bacteria were investigated in stools of 156 children under two years of age admitted to the pediatric wards of a general hospital at the western metropolitan area of santiago, chile, because of acute diarrhea with only one stool sample. a known pathogenic agent was isolated from 115 cases (73.7%), this being a bacteria in 87/115 (75.6%). most prevalent microorganisms were enteropathogenic escherichia coli (epec). (65.5%), specially serogroups 0111, 0119 and 055, enterotoxigenic escherichia coli ... | 1989 | 2520838 |
| amonabactin, a novel tryptophan- or phenylalanine-containing phenolate siderophore in aeromonas hydrophila. | aeromonas hydrophila 495a2 excreted two forms of amonabactin, a new phenolate siderophore composed of 2,3-dihydroxybenzoic acid, lysine, glycine, and either tryptophan (amonabactin t) or phenylalanine (amonabactin p). supplementing cultures with l-tryptophan (0.3 mm) caused exclusive synthesis of amonabactin t, whereas supplements of l-phenylalanine (0.3 to 30 mm) gave predominant production of amonabactin p. the two forms of amonabactin were separately purified by a combination of production an ... | 1989 | 2522922 |
| cutaneous manifestations of bacterial sepsis. | in short, bacterial sepsis is associated with a number of peripheral manifestations involving the skin and soft tissues. the pathogenesis of the lesions observed is not fully understood and is almost certainly multifactorial. in ecthyma gangrenosum, the presence of large numbers of gram-negative bacilli in the walls of small blood vessels without a substantial inflammatory response suggests that either the bacteria themselves or bacterial products are responsible for tissue damage. endotoxin pro ... | 1989 | 2523895 |
| genetics and molecular biology of siderophore-mediated iron transport in bacteria. | the possession of specialized iron transport systems may be crucial for bacteria to override the iron limitation imposed by the host or the environment. one of the most commonly found strategies evolved by microorganisms is the production of siderophores, low-molecular-weight iron chelators that have very high constants of association for their complexes with iron. thus, siderophores act as extracellular solubilizing agents for iron from minerals or organic compounds, such as transferrin and lac ... | 1989 | 2531838 |
| physiological control of amonabactin biosynthesis in aeromonas hydrophila. | amonabactin is a siderophore from aeromonas hydrophila which is produced in two biologically active forms composed of the phenolate 2,3-dihydroxybenzoic acid (dhb), lysine, glycine, and either trytophan (amonabactin t) or phenylalanine (amonabactin p). amonabactin biosynthetic mutants (generated by chemical mutagenesis) that either produced no amonabactin or overproduced the siderophore were isolated and identified on chrome azurol s siderophore detection agar. amonabactin-negative mutants were ... | 1989 | 2535179 |
| [infectious gastroenteritis in the immunocompetent child. significance of cryptosporidium spp. and aeromonas ssp]. | over a one-year period we have examined 390 stool specimens from 210 children hospitalized for infectious gastroenteritis or in whom infectious gastroenteritis occurred during hospitalization. the most prevalent pathogens were rotaviruses (22.4%), followed by enteric salmonella (8.1%), campylobacter jejuni/coli (2.4%), cryptosporidium spp. (2.4%) and aeromonas spp. (1.9%). both cryptosporidium spp. and aeromonas spp. are important pathogens of infectious gastroenteritis in immunocompetent childr ... | 1989 | 2540525 |
| [the occurrence of escherichia coli, aeromonas hydrophila, plesiomonas shigelloides and clostridium perfringens in the intestinal flora of gray herons (ardea cinerea)]. | the flora of the large intestine of 92 grey herons was examined for the frequency of aerobic and microaerobic growing bacteria. clostridium perfringens, aeromonas hydrophila, plesiomonas shigelloides and e. coli were isolated from 55%, 48%, 14% and 35% of the birds, respectively. it could be demonstrated that the findings of these bacteria in the intestinal flora are depending on the age of the birds. the percentage of carriers of clostridium perfringens, aeromonas hydrophila and plesiomonas shi ... | 1989 | 2548355 |
| protection of rainbow trout against vibriosis and furunculosis by the use of attenuated strains of vibrio anguillarum. | the fish pathogen vibrio anguillarum causes a lethal infection in rainbow trout (salmo gairdneri). three different avirulent mutants, constructed by transposon insertion mutagenesis (van20 and van70) or as antibiotic-resistant mutants (van1000), were isolated by screening 200 individual isolated mutants for avirulence. when used as live vaccines, all three avirulent mutants were able to induce protective immunity against the homologous as well as a heterologous strain of v. anguillarum. when van ... | 1989 | 2548446 |
| comparative in vitro activities of piperacillin-tazobactam and ticarcillin-clavulanate. | the in vitro activities of ticarcillin, piperacillin, clavulanic acid, tazobactam, ticarcillin-clavulanate, and piperacillin-tazobactam against 819 bacterial isolates were compared. the two beta-lactamase inhibitors, clavulanic acid and tazobactam, had little useful antibacterial activity but enhanced the activities of the penicillins against beta-lactamase-producing strains of haemophilus influenzae, branhamella catarrhalis, and methicillin-susceptible staphylococcus aureus; all strains were su ... | 1989 | 2552904 |
| relation between aeromonas and faecal coliforms in fresh waters. | a possible correlation between the presence of mesophilic aeromonads and the number of faecal coliforms present in three fresh-water habitats subject to differing levels of faecal pollution was investigated. concentration of aeromonas spp. between 10(2)-10(9) cfu/100 ml and faecal coliforms of between 9-10(7) cfu/100 ml were found in the waters. in water free from faecal pollution there was no correlation but in polluted waters there was a significant relationship between the numbers of aeromona ... | 1989 | 2553653 |
| enterotoxin induced diarrhoea--an update. | the pathogenic personality or the criteria required to be a successful pathogen, of enteric bacteria includes, among others, the ability to produce potent proteins which by different intracellular mechanisms elicit what we overtly see as diarrhoea. enteropathogens belonging to several genera like vibrio, escherichia, shigella, salmonella, campylobacter, aeromonas and yersinia include species capable of elaborating strikingly similar exotoxins which seem to share common mechanisms of action invol ... | 1989 | 2554547 |
| recovery of viruses and bacteria in waters off bondi beach: a pilot study. | a pilot study was conducted between february and april, 1989, on the occurrence of sewage-derived viruses and bacteria in the beach and nearshore waters off bondi, sydney. enteroviruses were isolated from 41% of a total of 66 sewage, sea-water, grease and sediment samples. poliovirus vaccine strains accounted for 78% of the isolates. adenoviruses were isolated four times and coxsackievirus b was isolated twice in samples that were collected away from the bathing area. rotavirus and hepatitis a v ... | 1989 | 2556634 |
| aeromonas species in aquaria: a reservoir of gastrointestinal infections? | to assess the role of aquaria in the epidemiology of aeromonas associated gastroenteritis, the prevalence and antibiotic susceptibility of aeromonads in ornamental aquaria were determined and compared to that of isolates obtained from patients with gastroenteritis. thirty-eight (76%) of 50 aquaria, including those located in our hospital, yielded 61 isolates: 24 (39.3%) aeromonas sorbria, 18 (29.0%) a. hydrophila, 15 (24.6%) a. caviae, and four (6.5%) which could not be speciated. in comparison, ... | 1989 | 2567310 |
| characterization of a pilus produced by aeromonas hydrophila. | a pilus produced by aeromonas hydrophila was purified and partially characterized. the pilin monomers had an apparent molecular weight of 17,000. agglutination studies indicated serological cross-reactivity in the pili of a. hydrophila strains. presence of pili did not correlate with hydrophobicity or haemagglutinating ability of the bacteria. | 1989 | 2569431 |
| motile aeromonas infection of striped (grey) mullet mugil cephalus. | infection of striped mullet (mugil cephalus) with aeromonas hydrophila results in an acute septicemic disease. the disease can be experimentally induced by intramuscular injection, skin or gill scarification or by the oral route using pellets purposely seeded with bacteria. the organism was isolated from the blood 1-2 days after infection and from all organs 24 hr or longer after infection. the disease is characterized by early inflammatory and proliferative changes and later necrotic changes. e ... | 1989 | 2575883 |
| conjugation system of incc plasmid ra1, and the interaction of ra1 pili with specific rna phage c-1. | ra1 was the only incc plasmid that was slightly temperature-sensitive for replication and transfer. at 30 degrees c, ra1 determined constitutive synthesis of conjugative pili and yet was transfer-repressed. attachment of shaft-adsorbing rna phage c-1 virions prevented the probable retraction of pili under heat stimulus (55 degrees c). electron microscopy showed single adsorbed virions at pilus bases where they were thought to have stopped retraction. | 1989 | 2576151 |
| bombesin: an activator of specific aeromonas antibody secretion in rat intestine. | the effect of bombesin (bbs) in modulating the secretion of specific aeromonas antibodies in rat intestine was determined. rats were immunized with the culture supernatant of aeromonas hydrophila, isolate ssu. this culture supernatant contained a number of toxins that may be considered virulence factors. after 24 days of immunization, rats were anesthetized and a 10-cm intestinal segment was perfused with phosphate-buffered saline. the effluents were collected for measurement of iga and igg by t ... | 1989 | 2582984 |
| chronic colitis after aeromonas infection. | 1989 | 2583575 | |
| hygienic status of food handlers. | the study demonstrated bacterial species on hands and nails of food-handlers before and after hand-washing. those were staphylococcus spp., streptococcus spp., micrococcus spp., bacillus spp., diphtheroid, aeromonas hydrophila, klebsiella pneumoniae, acinetobacter, enterobacter cloacae, escherichia coli, pseudomonas spp., proteus mirabilis, serratia spp., citrobacter freundii. before hand washing, each food-handler harboured one to eight bacterial species. after hand-washing (eight with water fr ... | 1989 | 2584903 |
| aeromonas hydrophila strains with moderate virulence. | a group of aeromonas hydrophila strains was obtained from very different origins which showed very similar characteristics (lipopolysaccharide, outer-membrane protein profile, toxin production, bacteriophage sensitivity and moderate virulence), besides the heterogenicity observed among the a. hydrophila strains. this group of a. hydrophila strains with moderate virulence can be easily recognized by their bacteriophage sensitivity pattern. | 1989 | 2593869 |
| the germ-spectrum problem and antibiotic-resistance in bida and minna hospitals--nigeria. | in the present paper the occurrence of different species of pseudomonas, of acinetobacter calcoaceticum var. anitratum and aeromonas hydrophila in hospitals is described. the possible human pathogenic significance is discussed. | 1989 | 2599385 |
| [infectious diarrhea in the adult]. | infectious diarrhoeas are usually divided into two types; toxinogenic and invasive. invasive diarrhoeas are copious and responsible for dehydration which is the principal clinical sign; mucosal lesions and bacteraemia are absent. the most typical of toxinogenic diarrhoeas is cholera, but enterotoxicogenic e. coli and aeromonas infections have similar clinical features. in invasive diarrhoeas the responsible microorganisms penetrate into the epithelial cells of the intestine, producing fever and ... | 1989 | 2602888 |
| mixed infections in childhood diarrhoea: results of a community study in kiambu district, kenya. | one thousand four hundred and twenty diarrhoea specimens from 846 children aged 0 to 60 months were collected and analysed for bacteria, parasites and rotavirus over a 16 month period, from june 1985 to september 1986 inclusive. the study was conducted in 4 villages situated in kiambu district, kenya. all the specimens were analysed for rotavirus and parasites, including cryptosporidium. the majority of the specimens were analysed for enteropathogenic escherichia coli (epec), shigella, salmonell ... | 1989 | 2606013 |
| some observations on the faecal carriage of mesophilic aeromonas species in cows and pigs. | replicate faecal samples from healthy individual pigs and cows were examined for the presence of aeromonas sp. over a 12-month period. aeromonads were found to be minor components of the faecal flora, only 8.8% of 520 samples from pigs and 4.6% of 481 samples from cows proving positive. isolation rates in both groups of animals were seasonal. a hydrophila (62% of the isolates) was the predominant species in cows, followed by a. caviae (32%) and a. sobria (15%). this pattern was also recorded in ... | 1989 | 2606161 |
| serogroups of aeromonas species from clinical and environmental sources in calcutta, india. | using a recently developed somatic o-antigen serogrouping scheme, 118 isolates of aeromonas spp. were serogrouped, 68 of which were isolated from diarrhoea patients admitted to the infectious diseases hospital and b.c. roy children's hospital, calcutta, from march 1987 to february 1988 and 50 randomly selected from an environmental study conducted during the same period. the isolates were typed into 23 different serogroups which represent 35.9% of the total 64 o-serogroups in the typing scheme. ... | 1989 | 2607101 |
| isolation of medically significant vibrio species from riverine sources in south east queensland. | vibrio and vibrio-like bacteria were isolated from water, sediments, plants and faeces from eight riverine sites in south east queensland, australia, using filtrations, enrichments and selective growth on thiosulphate-citrate-bile salts-sucrose (tcbs) and simidu agars. isolates (402 in toto) were classified by numerical analysis of phenotypic characteristics and comparison with fifteen reference cultures. of the isolates 41 (10.2%) were identified as vibrio cholerae, 33 (8.2%) as v. fluvialis an ... | 1989 | 2607994 |
| [are aeromonas species a cause of diarrheal diseases in yugoslavia?]. | isolation and identification of aeromonas spp. have been performed in 1,610 stools in the period from january 1987 to april 1988. of 1300 stool specimens taken from the patients with diarrheal syndrome (540 stools of children and 760 stools of adults) aeromonas spp. were isolated only in 4 patients (0.30%, p less than 0.001). positive stools were found in 3 adult patients and one child. in 310 examined stools of healthy adult persons aeromonas spp. were not isolated. a significantly higher incid ... | 1989 | 2609506 |