Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| differential behavior of vegf receptor expression and response to tnp-470 in two immortalized human endothelial cell lines. | angiogenesis consists of endothelial cell proliferation, migration and tube formation. it is useful to investigate endothelial cell behavior using immortalized endothelial cell lines. we characterized cell growth property, growth factor dependency and response to angioinhibitory drugs; tnp-470, staurosporine, radicicol and genistein, using human umbilical vein endothelial cells (huvecs) immortalized by human papilloma virus (hpv)-16 e6-e7, named huvecs/e6-e7, and huvecs/e6-e7 transformed by v-ki ... | 2000 | 10938394 |
| identification in humans of hpv-16 e6 and e7 protein epitopes recognized by cytolytic t lymphocytes in association with hla-b18 and determination of the hla-b18-specific binding motif. | human papilloma virus type 16 (hpv-16) is the hpv most frequently associated with cervical carcinoma in humans. for the prevention or treatment of cervical carcinoma, the e6 and e7 oncoproteins appear to be good targets for vaccine-induced cytotoxic t lymphocytes (ctl). lipopeptide vaccination is an efficient way of stimulating cellular responses. however, to synthesize effective lipopeptides, it is necessary to define which epitopes are immunogenic. in this study we first determined that peptid ... | 2000 | 10940919 |
| sexually transmitted infection and teenage sexuality. | the twin epidemics of sexually transmitted infection and nonmarital pregnancy and their consequences are two of the most significant issues faced by americans today. approximately 900,000 teenagers become pregnant each year. research indicates that these pregnancies often limit future educational and economic opportunities for mother, father, and child. sexually transmitted infections also pose a major threat to adolescents. for example, during a 3-year study of sexually active women at a major ... | 2000 | 10942466 |
| the human papillomavirus type 16 e6 and e7 oncoproteins cooperate to induce mitotic defects and genomic instability by uncoupling centrosome duplication from the cell division cycle. | loss of genomic integrity is a defining feature of many human malignancies, including human papillomavirus (hpv)-associated preinvasive and invasive genital squamous lesions. here we show that aberrant mitotic spindle pole formation caused by abnormal centrosome numbers represents an important mechanism in accounting for numeric chromosomal alterations in hpv-associated carcinogenesis. similar to what we found in histopathological specimens, hpv-16 e6 and e7 oncoproteins cooperate to induce abno ... | 2000 | 10944189 |
| association between activated k-ras and c-erbb-2 oncogenes with "high-risk" and "low-risk" human papilloma virus types in preinvasive cervical lesions. | clinical and epidemiological data have linked cervical cancer to the human papilloma virus (hpv) infection. however, the presence of hpv infection alone is not enough to cause tumorigenesis, suggesting a role for additional host-cell genetic factors. the aim of the present work was to study the association of k-ras and c-erbb-2 mutations in cervical tissue samples with different grades of dysplasia and infected with hpv-6 ("low-risk" type) or hpv-16 and hpv-18 ("high-risk" types). negative hpv-d ... | 2000 | 10946249 |
| penile hybrid verrucous-squamous carcinoma associated with a superficial inguinal lymph node metastasis. | a 62-year-old japanese man who presented with penile carcinoma is reported. the initial exophytic neoplasm excised from the coronal sulcus and prepuce on the abdominal side of the penis was diagnosed histologically as verrucous carcinoma. twenty-six months after the primary operation, an epithelial neoplasm recurred within the scar of the primary operation. the neoplasm histologically showed verrucous carcinoma and multiple invasive foci of conventional squamous cell carcinoma in the advancing e ... | 2000 | 10949460 |
| anal squamous intraepithelial lesions in human immunodeficiency virus-positive men and women. | studies from the era prior to the introduction of highly active antiretroviral therapy (haart) have shown that the prevalence of anal human papillomavirus (hpv) infection and anal squamous intraepithelial lesions (asil) was very high among human immunodeficiency virus (hiv)-positive homosexual men, and to a lesser extent, among hiv-negative homosexual men. prospective data also show that the incidence of high-grade asil (hsil), the putative invasive cancer precursor lesion, was high among both h ... | 2000 | 10950374 |
| erythroplasia of queyrat: coinfection with cutaneous carcinogenic human papillomavirus type 8 and genital papillomaviruses in a carcinoma in situ. | erythroplasia of queyrat is a carcinoma in situ that mainly occurs on the glans penis, the prepuce, or the urethral meatus of elderly males. up to 30% progress to squamous cell carcinoma. the cause of erythroplasia of queyrat is largely unknown. human papillomavirus type 16 dna has previously been detected only in very few distinctly characterized patients. we have investigated 12 paraffin-embedded biopsies from eight patients with penile erythroplasia of queyrat and control biopsies of inflamma ... | 2000 | 10951274 |
| connexin 43 expression is downregulated in raft cultures of human keratinocytes expressing the human papillomavirus type 16 e5 protein. | a decrease in gap junction-mediated cell-to-cell communication has previously been observed in monolayer cultures of human keratinocytes (hacat cells) expressing the human papillomavirus type 16 e5 (hpv16 e5) gene and attributed to the reduced phosphorylation of connexin 43, the most abundant connexin in hacat cells. in line with this observation, we have now analyzed the effect of hpv16 e5 on connexin 43 expression in raft cultures produced by transfected hacat cells. these keratinocytes transc ... | 2000 | 10955727 |
| iga antibodies to the 27-kda heat-shock protein in the genital tracts of women with gynecologic cancers. | heat-shock proteins promote cell survival under adverse environmental conditions. synthesis of the 27-kda (hsp27), 70-kda (hsp70), and 90-kda (hsp90) heat-shock proteins is increased in malignantly transformed cells and has been associated with tumor proliferation, metastasis, and resistance to chemotherapeutic agents. the increased expression of heat-shock proteins and their association with tumor-specific antigens may result in local immunity to the heat-shock proteins. we examined the occurre ... | 2000 | 10956393 |
| detection of human papillomavirus in laryngeal squamous dysplasia and carcinoma. an in situ hybridization and signal amplification study. | we examined the presence of human papillomavirus (hpv) dna in 65 cases of laryngeal squamous dysplasia and carcinomas using in situ hybridization with signal amplification in paraffin sections. hybridization was performed with biotinylated dna probes for hpv 6/11, 16/18, 31/33 and wide-spectrum hpv (6, 11, 16, 30, 31, 45, 51 and 52). hpv dna was found in 7 cases of the total sample (10.7%); it was also found in 4 out of 45 (8.8%) cases of invasive carcinoma and in 5 out of 33 (15.5%) cases of sq ... | 2000 | 10958409 |
| uncoupling between phenotypic senescence and cell cycle arrest in aging p21-deficient fibroblasts. | irreversible g(1) arrest in senescent human fibroblasts is mediated by two inhibitors of cyclin-dependent kinases (cdks), p21(cip1/sdi1/waf1) and p16(ink4a). to determine the physiological and molecular events that specifically require p21, we studied senescence in human diploid fibroblasts expressing the human papillomavirus type 16 e6 oncogene, which confers low p21 levels via enhanced p53 degradation. we show that in late-passage e6 cells, high cdk activity drives the cell cycle, but populati ... | 2000 | 10958672 |
| prediction of cervical intraepithelial neoplasia grade 2-3 using risk assessment and human papillomavirus testing in women with atypia on papanicolaou smears. | to determine whether human papillomavirus (hpv) dna testing and risk assessment can predict cervical intraepithelial neoplasia (cin) 2-3 on biopsies in women with atypia on papanicolaou smears. | 2000 | 10960635 |
| european course on hpv associated pathology: guidelines for primary care physicians for the diagnosis and management of anogenital warts. | the european course on hpv associated pathology (echpv) was founded in 1990 by a group of clinicians, pathologists, and virologists to teach important principles for the practice and management of human papillomavirus (hpv) disease to gynaecologists, dermatologists, and other medical disciplines. these guidelines are intended to assist the practice of primary care physicians for diagnosis and treatment of anogenital warts. | 2000 | 10961190 |
| human papillomavirus type 16 e6/e7-specific cytotoxic t lymphocytes in women with cervical neoplasia. | infection with oncogenic human papillomavirus (hpv) types is associated with the development of cervical neoplasia (cin). the e6 and e7 oncoproteins are constitutively expressed in these lesions and are therefore putative targets for the immune response against hpv. the relation between hpv 16-specific memory cytotoxic t-cell precursor (mctlp) activity to both oncoproteins and the natural course of cervical dysplasia was analyzed in 38 patients participating in a nonintervention cohort study of ... | 2000 | 10962445 |
| brca1 activation of the gadd45 promoter. | breast cancer susceptibility gene brca1 has been implicated in the control of gene regulation and such regulated genes are thought to mediate the biological role of brca1. overexpression of brca1 induces gadd45, a p53-regulated and stress-inducible gene. however, the molecular mechanism by which brca1 induces the expression gadd45 remains unclear. in this report, we have shown that the gadd45 promoter is strongly activated following expression of wild-type brca1. in contrast, both the tumor-deri ... | 2000 | 10962562 |
| casein kinase ii phosphorylation of the human papillomavirus-18 e7 protein is critical for promoting s-phase entry. | the human papillomavirus type 18 e7 protein subverts the prb/e2f pathway to promote s-phase reentry by postmitotic, differentiated primary human keratinocytes in support of viral dna amplification. we prepared a panel of hpv-18 e7 mutations in prb binding or in casein kinase ii (ckii) phosphorylation. our results showed that the ability of e7 binding to prb correlated with the activation of dna polymerase alpha or cyclin e to various extents in differentiated keratinocytes of organotypic culture ... | 2000 | 10965847 |
| immunotherapy of gynaecological high-risk human papilloma virus infection with human leukocyte ultrafiltrate. | in this preliminary presentation the outcome of women following systemic treatment of cervical human papilloma virus (hpv) with a leukocyte ultrafiltrate is reported. | 2000 | 10968593 |
| detection of human papillomavirus type 16 dna in consecutive genital samples does not always represent persistent infection as determined by molecular variant analysis. | persistent human papillomavirus (hpv) infection of the uterine cervix is a risk factor for progression to high-grade squamous intraepithelial lesions. detection in consecutive genital samples of hpv-16 dna, a frequently encountered hpv type, may represent persistent infection or reinfection. we undertook a study using pcr-single-strand conformation polymorphism (sscp) analysis and sequencing of pcr products (pcr-sequencing) to determine if consecutive hpv-16-positive samples contained the same h ... | 2000 | 10970388 |
| regulation of the 26s proteasome by adenovirus e1a. | we have identified the n-terminus of adenovirus early region 1a (ade1a) as a region that can regulate the 26s proteasome. specifically, in vitro and in vivo co-precipitation studies have revealed that the 19s regulatory components of the proteasome, sug1 (s8) and s4, bind through amino acids (aa) 4-25 of ad5 e1a. in vivo expression of wild-type (wt) ade1a, in contrast to the n-terminal ade1a mutant that does not bind the proteasome, reduces atpase activity associated with anti-s4 immunoprecipita ... | 2000 | 10970867 |
| preventive and therapeutic vaccines for human papillomavirus-associated cervical cancers. | 'high risk' genotypes of the human papillomavirus (hpv), particularly hpv type 16, are the primary etiologic agent of cervical cancer. thus, hpv-associated cervical malignancies might be prevented or treated by induction of the appropriate virus-specific immune responses in patients. sexual transmission of hpv may be prevented by the generation of neutralizing antibodies that are specific for the virus capsid. in ongoing clinical trials, hpv virus-like particles (vlps) show great promise as prop ... | 2000 | 10971133 |
| requirement of wild-type p53 protein for maintenance of chromosomal integrity. | chromosomal double-strand breaks (dsbs) occurring in mammalian cells can initiate genomic instability, and their misrepairs result in chromosomal deletion, amplification, and translocation, common findings in human tumors. the tumor-suppressor protein p53 is involved in maintaining genomic stability. in this study, we demonstrate that the deficiency of wild-type p53 protein may allow unrepaired dsbs to initiate chromosomal instability. the human lymphoblastoid cell line tk6-e6 was established by ... | 2000 | 10972990 |
| new technologies in cervical cancer precursor detection. | the current literature reflects three routes toward improving cervical cancer screening. the first is to improve the test qualities of cytology-based screening. the use of liquid-based cytology and computerized analysis of papanicolaou tests are examples of attempts at this approach. secondly, through various combinations of parallel or sequential tests, either the sensitivity or the specificity of a given test could be improved depending on the tests chosen and the order in which they were perf ... | 2000 | 10975554 |
| molecular identification of 7 human papillomavirus types in recurrent respiratory papillomatosis. | recurrent respiratory papillomatosis (rrp) is the most frequent benign neoplasm in childhood; it originates as a mild dysphonia and results in asphyxia. the rrp has been associated with an infection caused by human papillomavirus (hpv), mainly types 6 and 11, the latter being associated with more severe rrp. | 2000 | 10979126 |
| association of vitamin a deficiency with cervical squamous intraepithelial lesions in human immunodeficiency virus-infected women. | to explore the relationship between vitamin a (retinol) deficiency and cervical squamous intraepithelial lesions (sils) in human immunodeficiency virus (hiv)-infected women, we measured serum retinol concentrations in 1314 women enrolled in the women's interagency hiv study and correlated the results with concurrent cervical cytology. at the baseline visit, 204 (15.5%) of the 1314 patients had retinol concentrations consistent with deficiency (<1.05 micromol/l). analysis of papanicolaou smears s ... | 2000 | 10979903 |
| [human papillomavirus and the development of cervical cancer: concept of carcinogenesis]. | infection with high risk human papillomavirus (hrhpv) plays a central aetiological role in cervical cancer. still, cervical carcinogenesis is a multistep process which requires other events in addition to hrhpv infection. recent data have resulted in the following concept of cervical carcinogenesis: hrhpv infects normal squamous epithelium. in most cases this will not lead to a lesion or at worst give rise to a regressing low grade cervical intraepithelial neoplasia (cin). both phenomena involve ... | 2000 | 10981234 |
| telomerase activity in hpv-associated vulvar vestibulitis. | to find a possible correlation between telomerase activity, mean telomere length and human papillomavirus (hpv) presence and type in vulvar vestibulitis. | 2000 | 10986682 |
| pregnancy-associated invasive squamous cell carcinoma of the vulva in a 28-year-old, hiv-negative woman. a case report. | the incidence of invasive squamous cell carcinoma of the vulva in women under 40 years of age has been increasing, particularly in association with human papillomavirus. invasive vulvar carcinoma is rare in women under 30, as is an association with pregnancy. we report on a 28-year-old woman who was diagnosed with invasive squamous cell carcinoma of the vulva during pregnancy. | 2000 | 10986685 |
| human papillomavirus infection in danish female sex workers. decreasing prevalence with age despite continuously high sexual activity. | the prevalence of the human papillomavirus (hpv) has been found to decrease with age, but whether this applies to all populations is not clear. knowledge about the generalizability of this age dependency is important to understand the natural history of the infection, but may also have implications in relation to the use of hpv testing in cervical cancer screening. | 2000 | 10987448 |
| a population-based five-year follow-up study of cervical human papillomavirus infection. | the purpose of this study was to determine the long-term tendency for cervical human papillomavirus infections to persist in the general population. | 2000 | 10992174 |
| up-regulation of epidermal growth factor-receptors (egf-r) by nicotine in cervical cancer cell lines: this effect may be mediated by egf. | over-expression of epidermal growth factor-receptors (egf-r) has been described in a variety of cancers, including cervical cancer. nicotine may increase cellular proliferation rates through a mechanism involving egf or egf-r. in this study, we ascertain the effect of egf antibodies on nicotine-enhanced proliferation rates in two cervical cancer cell lines. | 2000 | 10994639 |
| human papillomavirus-associated cancers in patients with human immunodeficiency virus infection and acquired immunodeficiency syndrome. | human papillomavirus (hpv)-associated anogenital malignancies occur frequently in patients with human immunodeficiency virus (hiv) infection and the acquired immunodeficiency syndrome (aids). the purpose of our study was to determine if the high frequency of these cancers is due to lifestyle factors associated with both hpv and hiv infections or to immunosuppression following hiv infection. | 2000 | 10995805 |
| amplification with molecular beacon primers and reverse line blotting for the detection and typing of human papillomaviruses. | a novel method for the detection and typing of human papillomavirus (hpv) was developed using molecular beacon primers. the method is based on the use of hpv-specific primers containing a hairpin loop structure in which fluorescent donor and quencher groups are held in close proximity such that fluorescence is quenched. amplification of the target sequence results in the opening of the loop and the resulting fluorescence can be detected on a sequence detector system (sds) 7700 (applied biosystem ... | 2000 | 10996637 |
| a recombinant vaccinia virus containing the papilloma e2 protein promotes tumor regression by stimulating macrophage antibody-dependent cytotoxicity. | human papillomavirus infection is associated with cervical cancer. the e6 and e7 papillomavirus proteins are normally required for the maintenance of the malignant phenotype. expression of these proteins in infected cells is negatively regulated by the binding of the papilloma e2 protein to the long terminal control region of the papilloma virus genome. the e2 protein can also promote cell arrest and apoptosis in hela cells. therefore, it is clear that this protein has the potential of inhibitin ... | 2000 | 10999461 |
| usefulness of human papilloma virus testing in the screening of cervical cancer precursor lesions: a retrospective study in 314 cases. | to assess the usefulness of human papilloma virus (hpv) typing for predicting pre-malignant and malignant cervical lesions. | 2000 | 11000508 |
| validity of adolescent and young adult self-report of papanicolaou smear results. | to assess the validity of adolescent and young adult report of papanicolaou smear results and to determine sociodemographic, cognitive, and behavioral factors associated with incorrect reporting. | 2000 | 11004371 |
| primary cervical carcinomas show 2 common regions of deletion at 3p, 1 within the fhit gene: evaluation of allelic imbalance at fhit, rb1 and tp53 in relation to survival. | chromosome arm 3p is re-arranged in many tumor types, including cervical carcinomas. putative tumor-suppressor genes on 3p have been proposed, including the fhit gene, which maps to chromosome band 3p14.2. we have analyzed 79 primary cervical carcinomas for allelic imbalance (ai) at 17 chromosome 3 loci, including 3 within the fhit gene. expression of the fhit gene was evaluated after immunohistochemistry with an antibody against the pfhit protein. previously determined human papillomavirus stat ... | 2000 | 11004671 |
| differential distribution of sequence variations in hpv-16 e6. | the e6 regions of the oncogenic human papillomaviruses (hpvs) are important in carcinogenesis and immune recognition. we examined the e6 dna sequence from hpv-16-associated cervical cancers to determine the frequency and degree of variation from the consensus sequence in selected populations. | 2000 | 11006023 |
| significance of atypical squamous cells of undetermined significance on thinprep papanicolaou smears. | the aim of this study was to assess the prevalence and risk factors predictive of dysplasia among women seen in a gynecologic oncology service with the cytologic diagnosis of atypical squamous cells of undetermined significance (ascus) on papanicolaou smears obtained by the thinprep method. | 2000 | 11006029 |
| correlates of the spread of human papillomavirus infection. | knowledge of the correlates of human papillomavirus (hpv) seropositivity is of interest for planning of preventive measures and for evaluation of possible confounding in epidemiological studies. the epidemiological determinants for seropositivity for oncogenic and benign hpv types were assessed using a serosurvey of 275 healthy swedish women, stratified by age and lifetime number of sexual partners. seroprevalences were compared with 17 behavioral variables obtained by interview and 3 laboratory ... | 2000 | 11008914 |
| the prevalence of genital human papillomavirus infections in abused and nonabused preadolescent girls. | to compare the prevalence of genital human papillomavirus (hpv) infections in sexually abused and nonabused preadolescent girls and assess the feasibility of conducting a longitudinal study of the natural history of hpv infection in this population. | 2000 | 11015503 |
| use of hormone replacement therapy (hrt) and detection of human papillomavirus (hpv) dna in postmenopausal women. | purpose: this study investigated the association between detection of hpv dna among current and past hrt users compared to never hrt users, duration of hrt use, and type of hrt (estrogen, estrogen/progestin).methods: postmenopausal women (n = 390) were recruited from a university hospital and completed a questionnaire regarding 1) hrt use, duration, and type, 2) reproductive and sexual history, 3) smoking and alcohol use, and 4) hpv-related diseases. cervical specimens were obtained for pap smea ... | 2000 | 11018386 |
| detection of human papillomavirus in epithelial lesions of the conjunctiva. | many factors like exposure to uv radiation, climatic conditions, genetic predisposition, immunological state and, more recently, the presence of hpv have been implicated in the genesis of some lesions of the conjunctiva, especially the carcinoma. | 2000 | 11018845 |
| treatment of external genital warts in men with imiquimod 2% in cream. a placebo-controlled, double-blind study. | the purpose of this double-blind, placebo-controlled study was to evaluate the safety, clinical efficacy and tolerability of imiquimod (2%) in cream to cure external genital warts in males. | 2000 | 11023759 |
| the e1e4 protein of human papillomavirus type 16 associates with a putative rna helicase through sequences in its c terminus. | human papillomavirus type 16 (hpv16) infects cervical epithelium and is associated with the majority of cervical cancers. the e1e4 protein of hpv16 but not those of hpv1 or hpv6 was found to associate with a novel member of the dead box protein family of rna helicases through sequences in its c terminus. this protein, termed e4-dbp (e4-dead box protein), has a molecular weight of 66,000 (66k) and can shuttle between the nucleus and the cytoplasm. it binds to rna in vitro, including the major hpv ... | 2000 | 11024137 |
| a longitudinal study of hpv detection and cervical pathology in hiv infected women. | to monitor the presence and persistence of high risk (hr) human papillomavirus (hpv) in cervical brushings from hiv infected women. | 2000 | 11026879 |
| histological diagnosis of cutaneous "warty" carcinoma on a pre-existing hpv lesion. | a middle-aged man with an 8-year history of a fungating tumour mass on his thigh was histologically diagnosed as having an invasive "warty" carcinoma at the location of a pre-existing human papillomavirus (hpv) lesion. the tumour surface had a verruciform appearance with papillae containing fibrovascular cores. many of the malignant cells displayed changes consistent with koilocytotic atypia. we noticed a greater degree of nuclear atypia in comparison with a verrucous carcinoma. focally, some ne ... | 2000 | 11028866 |
| bovine dental papilla-derived cells immortalized with hpv 18 e6/e7. | in vitro investigations of cell-specific metabolism and cell interactions as well as biocompatibility studies are often hampered by the limited lifespan of primary cells originating from target tissues like the oral mucosa, gingiva or pulp. pulp cells, as do other primary cells, undergo senescence after several passages in vitro. however, senescence can be overcome by transfection of primary cells with oncogenes like the hpv 18 (human papillomavirus 18) e6/e7 oncogene, resulting in immortalized ... | 2000 | 11037760 |
| cell transformation by the e7 oncoprotein of human papillomavirus type 16: interactions with nuclear and cytoplasmic target proteins. | the e7 oncoprotein of human papillomavirus type 16 (hpv-16) has long been known as a potent immortalizing and transforming agent. however, the molecular mechanisms underlying cell transformation and immortalization by e7 remain largely unknown. it is believed that e7 exerts its oncogenic function at least in part by modulating cellular growth regulatory pathways. increasing experimental evidence suggests that cell transformation by e7 is mediated by the physical association of e7 with cellular r ... | 2000 | 10547667 |
| the human papillomavirus (hpv) 16 e2 protein induces apoptosis in the absence of other hpv proteins and via a p53-dependent pathway. | the human papillomavirus (hpv) e2 protein regulates viral gene expression and is also required for viral replication. hpv-transformed cells often contain chromosomally integrated copies of the hpv genome in which the viral e2 gene is disrupted. we have shown previously that re-expression of the hpv 16 e2 protein in hpv 16-transformed cells results in cell death via apoptosis. here we show that the hpv 16 e2 protein can induce apoptosis in both hpv-transformed and non-hpv-transformed cell lines. ... | 2000 | 10617590 |
| molecular cloning and nucleotide sequence analysis of a novel human papillomavirus (type 82) associated with vaginal intraepithelial neoplasia. | the genome of a novel human papillomavirus (hpv-82) was cloned from a vaginal intraepithelial neoplasia grade i. in our series of 291 biopsy specimens, hpv-82 was identified in one case each of cervical intraepithelial neoplasia grade ii and grade iii by blot hybridization. the histological localization of hpv-82 dna in the three lesions was confirmed by in situ hybridization. the results indicated that hpv-82 is an etiologic agent for vaginal and cervical intraepithelial neoplasia. by nucleotid ... | 2000 | 10618284 |
| hpv16 e6 oncogene variants in women with cervical intraepithelial neoplasia. | human papillomaviruses (hpvs) are strongly associated with the development of high grade cervical intraepithelial neoplasia (cin) and cervical carcinoma, with between 40-80% of patients with cervical carcinoma being attributed to a single hpv type, hpv16 depending on the methods used and geographical location of the particular study [van den brule et al., 1996]. an hpv16 e6 variant has been described which is strongly associated with high grade cin [ellis et al., 1997] and with the human leukocy ... | 2000 | 10630967 |
| hpv dna testing of self-collected vaginal samples compared with cytologic screening to detect cervical cancer. | more than half of the women diagnosed as having cervical cancer in the united states have not been screened within the last 3 years, despite many having had contact with the health care system. in many other regions of the world, there is only limited access to cervical cancer screening. | 2000 | 10632284 |
| immunogenicity of the b monomer of escherichia coli heat-labile toxin expressed on the surface of streptococcus gordonii. | the b monomer of the escherichia coli heat-labile toxin (ltb) was expressed on the surface of the human oral commensal bacterium streptococcus gordonii. recombinant bacteria expressing ltb were used to immunize balb/c mice subcutaneously and intragastrically. the ltb monomer expressed on the streptococcal surface proved to be highly immunogenic, as ltb-specific immunoglobulin g (igg) serum titers of 140,000 were induced after systemic immunization. most significantly, these antibodies were capab ... | 2000 | 10639444 |
| induction of cell-cycle arrest in cervical cancer cells by the human immunodeficiency virus type 1 viral protein r. | to determine the ability of the human immunodeficiency virus type 1 (hiv-1) gene vpr to induce cell-cycle arrest in cervical cancer cells with or without human papillomavirus (hpv) type 16 e6 or e7 expression. | 2000 | 10636517 |
| p53 alterations in recurrent squamous cell cancer of the head and neck refractory to radiotherapy. | the aim of the study was to determine the incidence of p53 alterations by mutation, deletion or inactivation by mdm2 or human papillomavirus (hpv) infection in recurrent squamous cell cancer of the head and neck (scchn) refractory to radiotherapy. twenty-two tumours were studied. the p53 status of each tumour was analysed by sequencing of exons 4-9 and by immunohistochemistry. mdm2 expression was assessed by immunohistochemistry and hpv infection was assessed by polymerase chain reaction of tumo ... | 2000 | 10646894 |
| purification of his-tagged proteins by immobilized chelate affinity chromatography: the benefits from the use of organic solvent. | recombinant proteins overexpressed in and purified from escherichia coli contain impurities that are toxic in biological assays. the application of affinity purification procedures is often not sufficient to remove these toxic components. we here describe a simple and fast, one-step protocol to remove these impurities highly efficiently. four recombinant proteins were overexpressed in e. coli as his-tagged fusion proteins and purified by immobilized metal chelate affinity chromatography on ni-nt ... | 2000 | 10648174 |
| antibodies against oncoproteins e6 and e7 of human papillomavirus types 16 and 18 in cervical-carcinoma patients from russia. | certain human papillomaviruses (hpv), mainly types 16 and 18, have been widely recognized as an essential etiologic factor for the development of carcinoma of the uterine cervix. the early hpv proteins e6 and e7 are consistently expressed in the tumor cells, and cervical-carcinoma patients can develop antibodies against these oncoproteins. for cervical-carcinoma patients from eastern europe and russia, detailed information on hpv dna prevalence and hpv-specific immune responses is limited. the p ... | 2000 | 10652419 |
| a novel and rapid pcr-based method for genotyping human papillomaviruses in clinical samples. | many human papillomavirus (hpv) genotypes are associated with cervical carcinoma. we demonstrate the utility of an innovative technique for genotyping of hpv in cervical tissue samples. this method provides an accurate means of identification of the specific hpv genotypes present in clinical specimens. by using the my09-my11 and the gp5(+)-gp6(+) consensus primer pairs, hpv sequences were amplified by nested pcr from dna isolated from cervical smear samples. this led to the production of an appr ... | 2000 | 10655368 |
| human papillomavirus types 16 e6 and e7 contribute differently to carcinogenesis. | high-risk human papillomaviruses (hpvs) are etiologically implicated in human cervical cancer. two viral genes, e6 and e7, are commonly found expressed in these cancer cells. we have previously shown that mice transgenic for the hpv-16 e6 gene or e7 gene, in which the e6 or e7 was expressed in the basal layer of epithelia, developed skin tumors. the spectrum of tumors derived from e6 and e7 mice differed, however; although most tumors derived from the e7-transgenic mice were benign, the majority ... | 2000 | 10662610 |
| prevalence of and risks for cervical human papillomavirus infection and squamous intraepithelial lesions in adolescent girls: impact of infection with human immunodeficiency virus. | data suggest that in adults, human papillomavirus (hpv) infections and their sequalae, squamous intraepithelial lesions (sils), occur more commonly among human immunodeficiency (hiv)-infected women because of the hiv-associated cd4+ t-cell immunosuppression. since adolescents are more likely to be early in the course of hiv and hpv infections, the study of both infections in this age group may help elucidate their initial relationship. | 2000 | 10665598 |
| use of fluorogenic histocompatibility leukocyte antigen-a*0201/hpv 16 e7 peptide complexes to isolate rare human cytotoxic t-lymphocyte-recognizing endogenous human papillomavirus antigens. | cervical cancer (cacx) is the second most common female malignancy worldwide and remains a clinical problem despite improvements in early detection and therapy. cacx and preinvasive cervical intraepithelial neoplasia (cin3) are strongly associated with infection by human papillomavirus (hpv), particularly types 16 and 18. two nonstructural viral proteins, e6 and e7, are constitutively expressed in cervical tumors and are crucial for the maintenance of the transformed phenotype. these proteins th ... | 2000 | 10667589 |
| memorandum for: science writers and editors on the journal press list: limited potential for human papillomavirus (hpv) testing for women with low-grade pap smears | 2000 | 10699059 | |
| inactivation of interferon regulatory factor-1 tumor suppressor protein by hpv e7 oncoprotein. implication for the e7-mediated immune evasion mechanism in cervical carcinogenesis. | in studying biological roles of interferon regulatory factor (irf)-1 tumor suppressor in cervical carcinogenesis, we found that hpv e7 is functionally associated with irf-1. binding assays indicate a physical interaction between irf-1 and hpv e7 in vivo and in vitro. the carboxyl-terminal transactivation domain of irf-1 was required for the interaction. transient co-expression of e7 significantly inhibits the irf-1-mediated activation of ifn-beta promoter in nih-3t3 cells. co-transfection of e7 ... | 2000 | 10702232 |
| synthesis of viral dna and late capsid protein l1 in parabasal spinous cell layers of naturally occurring benign warts infected with human papillomavirus type 1. | we investigated human papillomavirus type 1 (hpv1)-specific transcription, viral dna replication, and viral protein expression in naturally occurring benign tumors by in situ hybridization, 5-bromodeoxyuridine (brdu) incorporation, and immunohistochemistry and obtained results different from other hpv-infected benign tumors characterized so far. moderate amounts of transcripts with a putative coding potential for e6/e7, e1, and e2 were demonstrated from the first subrabasal cell layer throughout ... | 2000 | 10704337 |
| enhancement of dna vaccine potency by linkage of antigen gene to an hsp70 gene. | nucleic acid vaccines represent an attractive approach to generating antigen-specific immunity because of their stability and simplicity of delivery. however, there is still a need to increase the potency of dna vaccines. using human papillomavirus type 16 e7 as a model antigen, we evaluated the effect of linkage to mycobacterium tuberculosis heat shock protein 70 (hsp70) on the potency of antigen-specific immunity generated by naked dna vaccines. we found that vaccines containing e7-hsp70 fusio ... | 2000 | 10706121 |
| knowledge of human papillomavirus infection among young adult men and women: implications for health education and research. | human papillomavirus (hpv) infection of the genital tract is one of the most common sexually transmitted diseases (stds), and a subset of genital tract hpvs are etiologically associated with cervical cancer. the prevalence of hpv infection is highest among adolescents and young adults. this study was undertaken to explore first year college students' knowledge about hpvs and to determine whether there were gender differences in this knowledge. an anonymous survey was distributed to all first yea ... | 2000 | 10706210 |
| combined interferon-gamma and tumor necrosis factor-alpha treatment differentially affects adhesion and migration of keratinocyte-derived cells to laminin-1. | interactions with the extracellular matrix constitute basic steps in cervix carcinoma cell invasion. in this study, we examined the adhesion and migration profiles of two human papillomavirus (hpv) dna-transfected keratinocyte-derived cell lines, eil8 and 18-11s3, and of the cervix adenocarcinoma siha cell line, towards laminin-1, and the selective effect of a 24-72 h treatment of 1000 u/ml interferon-gamma (ifn-gamma) and tumor necrosis factor-alpha (tnf-alpha), a treatment that significantly d ... | 2000 | 10714393 |
| population-based study of human papillomavirus infection and cervical neoplasia in rural costa rica. | human papillomavirus (hpv) is the main cause of cervical neoplasia. because few population-based studies have investigated the prevalence of type-specific infection in relation to cervical disease, we studied a high-risk population, estimating the prevalence of hpv infection and the risk associated with various hpv types. | 2000 | 10716964 |
| [detection of human papillomavirus in cutaneous extragenital bowen's disease in immunocompetent patients]. | a specific link between human papillomavirus (hpv) types 16, 18, 31, and 33 and genital carcinomas and between hpv type 5 and cutaneous extragenital carcinomas in patients with epidermodysplasia verruciformis and renal transplant has been previously found. the aim of this prospective study was to detect hpv in cases of cutaneous extragenital bowen's disease (bd) from non-immunosuppressed patients. | 2000 | 10717561 |
| cytokine profile in genital tract secretions from female adolescents: impact of human immunodeficiency virus, human papillomavirus, and other sexually transmitted pathogens. | quantitative enzyme-linked immunosorbent assays were used to measure interleukin (il)-2, il-10, and il-12 in cervical secretions from female adolescents with and without sexually transmitted infections. compared with human immunodeficiency virus [hiv]-negative patients, hiv-positive patients had higher concentrations of il-10 (118.2 pg/ml vs. 34.5 pg/ml; p=.002) and il-12 (175.5 pg/ml vs. 85.1; p=.03). il-2 concentrations were not statistically different. furthermore, genital tract infections we ... | 2000 | 10720516 |
| development, characterization and distribution of adoptively transferred peripheral blood lymphocytes primed by human papillomavirus 18 e7--pulsed autologous dendritic cells in a patient with metastatic adenocarcinoma of the uterine cervix. | we describe a 27-year-old woman with systemic chemoresistant and radioresistant metastatic disease secondary to a recurrence of human papillomavirus (hpv) 18 infected cervical adenocarcinoma of the uterine cervix who received adoptive transfer of peripheral blood t cells stimulated with hpv 18 e7-pulsed autologous dendritic cells (dc). extensive in vitro characterization of the dc-activated t cells derived from peripheral blood mononuclear cells (pbmc) included phenotypic analysis, cytotoxicity ... | 2000 | 10726612 |
| the roles of e6-ap and mdm2 in p53 regulation in human papillomavirus-positive cervical cancer cells. | the p53 tumor suppressor is regulated by the mdm2 oncoprotein through a negative feedback mechanism. mdm2 promotes the ubiquitination and proteasome-dependent degradation of p53, possibly by acting as a ubiquitin ligase. in cervical cancer cells containing high-risk human papillomaviruses (hpv), p53 is also targeted for degradation by the hpv e6 oncoprotein in combination with the cellular e6-ap ubiquitin ligase. in this report, we describe the identification of efficient antisense oligonucleoti ... | 2000 | 10726657 |
| the amyloid beta peptide abeta (25-35) induces apoptosis independent of p53. | apoptosis of neuronal cells apparently plays a role in alzheimer's disease (ad). the amyloid beta (abeta) peptide derived from beta-amyloid precursor protein is found in ad brain in vivo and can induce apoptosis in vitro. while p53 accumulates in cells of ad brain, it is not known if p53 plays an active role in abeta-induced apoptosis. we show here that inactivation of p53 in two experimental cell lines, either by expression of the papillomavirus e6 protein or by a shift to restrictive temperatu ... | 2000 | 10734238 |
| development and assessment of a general theory of cervical carcinogenesis utilizing a severe combined immunodeficiency murine-human xenograft model. | currently, we lack a theoretical explanation for why squamous cell cervical cancer develops predominantly in specific sites (i.e., along the squamocolumnar junction). we therefore implanted human cervical tissues containing the transformation zone in severe combined immunodeficiency (scid) mice and studied morphology, steroid effects, gene expression, and human papillomavirus (hpv) factors. | 2000 | 10739703 |
| 5-fluorouracil-induced apoptosis in cultured oral cancer cells. | chemotherapy is commonly used to treat advanced oral squamous cell carcinoma (scc) and is known to kill cancer cells through apoptosis. our hypothesis states that 5-fluorouracil (5fu) also kills cultured oral epithelial cells through programmed cell death or apoptosis. cultured oral cancer cells were exposed to an optimum dose of 20 mg/ml of 5fu. cells were analyzed for changes in cell cycle distribution and induction of cell death including apoptosis. normal control, human papilloma virus-immor ... | 2000 | 10745178 |
| epstein-barr virus (ebv) genes expression in cervical intraepithelial neoplasia and invasive cervical cancer: a comparative study with human papillomavirus (hpv) infection. | to elucidate a causative role of epstein-barr virus (ebv) for cervical cancer, presence and expression of ebv genes were examined in 31 cervical carcinomas (icc), 23 cervical intraepithelial neoplasias (cin), and 35 normal cervices (ncx). in reverse transcription polymerase chain reaction (rt-pcr) analysis, eber-1 mrnawas expressed in 74% (23/31) of icc, 83% (19/23) of cin, 37% (13/35) of ncx. lmp-1 was expressed in 52% (16/31) of icc, 70% (16/23) of cin, and 23% (8/35) of ncx, and ebna-2 was ex ... | 2000 | 10746674 |
| role of routine human papillomavirus subtyping in cervical screening. | human papillomavirus testing has been evaluated as an adjunct to cervical cytologic screening. there is a well-established association between high-risk malignant subtypes of human papillomavirus infection and the development of neoplastic change. although human papillomavirus infection is a necessary condition for malignant transformation, it is not a sufficient condition. multiple cofactors are necessary for progression to dysplasia and then to invasive cervical cancer. because of the multifac ... | 2000 | 10752510 |
| p53 codon 72 polymorphism and susceptibility to skin cancer after renal transplantation. | infection with human papillomavirus (hpv) is an important risk factor for the development of skin cancer after renal transplantation. it has recently been suggested that degradation of the tumor suppressor gene p53 is an important mechanism for human papillomavirus-induced carcinogenesis. a common genomic polymorphism occurs at codon 72 of the p53 gene, and in vitro the codon 72arg variant appears to be particularly susceptible to degradation. | 2000 | 10755564 |
| advances in cervical screening technology. | the pap smear unquestionably is a successful screening test for cervical cancer. however, recent advances in technology have raised questions regarding whether the conventional pap smear is still the standard of care. this article relates issues of screening and cost-effectiveness to the state of the art in thin layer preparations, cytology automation, human papillomavirus screening, human papillomavirus vaccines, and other cervical screening adjuncts. perhaps nowhere in medicine is clinical dec ... | 2000 | 10757338 |
| human papilloma virus in melanoma biopsy specimens and its relation to melanoma progression. | to evaluate melanoma biopsy specimens for human papilloma virus (hpv) and determine the relation between the presence of hpv, in vitro growth, and clinical progression of melanoma in the patients from whom the biopsy specimens were derived. | 2000 | 10767787 |
| [effects of human papilloma virus types on course of juvenile respiratory papillomatosis and effectiveness of interferon therapy in children]. | identification of the type of human papilloma virus (hpv) was done by means of polymerase chain reaction in 40 children with juvenile respiratory papillomatosis (jrp). adjuvant to surgery interferon treatment was carried out in 18 patients (study group) with severe jrp. the rest 22 patients (control group) with less severe papillomatosis received only surgical treatment. the majority of the patients from both the groups had the virus type 6/11 (72.2 and 77.3% for the study and control groups, re ... | 2000 | 10771610 |
| human papillomavirus type 16 e7 oncoprotein represses transcription of human fibronectin. | the e7 oncoprotein encoded by human papillomavirus (hpv) type 16 repressed the transcription of fibronectin, a key component of the extracellular matrix. this repression, detected in several hpv-positive nontumorigenic and tumorigenic cell lines, was abolished when the cys-x-x-cys repeats in e7 were disrupted. | 2000 | 10775635 |
| human papillomaviruses and vulvar vestibulitis. | to assess the relationship between human papillomavirus (hpv) infection and vulvar vestibulitis syndrome. | 2000 | 10775729 |
| wild-type p53 transactivates the killer/dr5 gene through an intronic sequence-specific dna-binding site. | killer/dr5, a tumor necrosis factor-related apoptosis-inducing ligand (trail) death receptor gene, has been shown to be induced by dna damaging agents and radiation in a p53-dependent manner. although trail is a potential therapeutic agent for cancer, the induction mechanism of its receptors is poorly understood. here we show the identification of three p53 dna-binding sites in the killer/dr5 genomic locus located upstream (bs1; -0.82 kb) of the atg site, within intron 1 (bs2; +0.25 kb downstrea ... | 2000 | 10777207 |
| mutagenesis of the prb pocket reveals that cell cycle arrest functions are separable from binding to viral oncoproteins. | the pocket domain of prb is required for prb to arrest the cell cycle. this domain was originally defined as the region of the protein that is necessary and sufficient for prb's interaction with adenovirus e1a and simian virus s40 large t antigen. these oncoproteins, and other prb-binding proteins that are encoded by a variety of plant and animal viruses, use a conserved lxcxe motif to interact with prb. similar sequences have been identified in multiple cellular prb-binding proteins, suggesting ... | 2000 | 10779361 |
| characterization of pdz-binding kinase, a mitotic kinase. | hdlg, the human homologue of the drosophila discs-large (dlg) tumor suppressor protein, is known to interact with the tumor suppressor protein apc and the human papillomavirus e6 transforming protein. in a two-hybrid screen, we identified a 322-aa serine/threonine kinase that binds to the pdz2 domain of hdlg. the mrna for this pdz-binding kinase, or pbk, is most abundant in placenta and absent from adult brain tissue. the protein sequence of pbk has all the characteristic protein kinase subdomai ... | 2000 | 10779557 |
| a simplified and reliable hpv testing of archival papanicolaou-stained cervical smears: application to cervical smears from cancer patients starting with cytologically normal smears. | the efficacy of four methods to recover dna from papanicolaou (pap)-stained archival cervical smears for optimal detection of human papillomavirus (hpv) dna by gp5+/biogp6+ polymerase chain reaction (pcr) was investigated. two of the methods were based on proteinase k treatment and two based on treatment with guanidinium thiocyanate (gtc). the quality of the dna as measured by pcr assays amplifying different sizes of the beta-globin gene appeared to be superior for the gtc-based assays. using co ... | 2000 | 10780521 |
| widespread elevated expression of the human papilloma virus (hpv)-activating cellular transcription factor brn-3a in the cervix of women with cin3 (cervical intraepithelial neoplasia stage 3). | 2000 | 10781392 | |
| prognostic implication of human papillomavirus infection in squamous cell carcinoma of the lung. | on the subtropical island of okinawa, squamous cell carcinoma (scc), particularly the well-differentiated form, is the most frequent type of lung cancer, while this form is relatively rare on the japanese mainland and in other countries. furthermore, in okinawa, in 1993, 80% of scc cases of the lung were found to be infected with human papillomavirus (hpv). we studied the prognosis of scc of the lung with hpv infection (n = 25) and compared it with non-hpv-infected scc (n = 16). using the kaplan ... | 2000 | 10782464 |
| cancer in husbands of cervical cancer patients. | we used the swedish family-cancer database to analyze the spectrum of cancers diagnosed in husbands of women with in situ or invasive cervical cancer, and we compared these to second carcinogenic events in women presenting with these cancers. our hypothesis was increased cancer susceptibility from human papilloma virus (hpv). when the wives had in situ or invasive cervical cancer, the standard incidence ratios (sirs) for anal cancer in husbands were 1.75 (95% ci = 1.05-2.62) and 1.92 (95% ci = 0 ... | 2000 | 10784257 |
| retinoic acid resistance at late stages of human papillomavirus type 16-mediated transformation of human keratinocytes arises despite intact retinoid signaling and is due to a loss of sensitivity to transforming growth factor-beta. | in our in vitro model of human cell carcinogenesis, normal human foreskin keratinocytes (hkc) transfected with human papillomavirus type 16 dna (hkc/hpv16) progress toward malignancy through several phenotypically defined and reproducible "steps" that include immortalization, growth factor independence (hkc/gfi), differentiation resistance (hkc/dr), and ultimately malignant conversion. while hkc/hpv16 are very sensitive to growth inhibition by all-trans-retinoic acid (ra) at early passages, they ... | 2000 | 10792999 |
| genetic and epigenetic changes in human epithelial cells immortalized by telomerase. | exogenous expression of htert, the catalytic component of telomerase, is sufficient for the immortalization of human fibroblasts but insufficient for the immortalization of human foreskin keratinocytes (hfks) and human mammary epithelial cells (hmecs). these latter cell types can overcome senescence by coexpression of htert and human papillomavirus (hpv) e7 or by expression of htert and loss of p16(ink4a) expression, indicating that the retinoblastoma (rb) pathway, along with a telomere maintena ... | 2000 | 10793065 |
| folding of a dimeric beta-barrel: residual structure in the urea denatured state of the human papillomavirus e2 dna binding domain. | the dimeric beta-barrel is a characteristic topology initially found in the transcriptional regulatory domain of the e2 dna binding domain from papillomaviruses. we have previously described the kinetic folding mechanism of the human hpv-16 domain, and, as part of these studies, we present a structural characterization of the urea-denatured state of the protein. we have obtained a set of chemical shift assignments for the c-terminal domain in urea using heteronuclear nmr methods and found region ... | 2000 | 10794423 |
| p53 codon 72 polymorphism does not affect the risk of cervical cancer in patients from northern italy. | a case-control study was performed to investigate the risk of cervical cancer associated with p53 polymorphism at codon 72, encoding either arginine or proline. it has been recently suggested that the arginine isoform increases the susceptibility to invasive cervical cancer; however, data remain controversial. the polymorphism was examined by both allele-specific pcr and rflp analysis in 101 patients with primary cervical cancer and in 140 healthy women of the same age and from the same geograph ... | 2000 | 10794489 |
| differential cell cycle response of nontumorigenic and tumorigenic human papillomavirus-positive keratinocytes towards transforming growth factor-beta1. | human papillomaviruses (hpvs) are causative agents of a number of malignancies in humans, including cervical cancer. their tumorigenic potential is linked to expression of the viral e6/e7 genes which can interfere with normal cell cycle control by targeting p53, p21waf1, p27kip1, and prb. we show here that nontumorigenic and tumorigenic hpv-positive keratinocytes (hpk) exhibit striking differences in the response of cell cycle regulatory genes towards transforming growth factor beta-beta1. treat ... | 2000 | 10794545 |
| analysis of mutations in the urr and e6/e7 oncogenes of hpv 16 cervical cancer isolates from central china. | high rates of cervical cancer have been reported from parts of china and this may reflect a predominance of cervical infection with particularly aggressive human papillomavirus (hpv) variants. this pcr-based investigation of cervical tumours from sichuan province in central china demonstrated an hpv positivity rate of 88%. hpv 16 was most common (21/34, 61%), followed by hpv 18 (3/34, 9%), while types 33, 45, 58 and 59 were each identified in one specimen. sequencing of up to 1349 bases of the 2 ... | 2000 | 10797293 |
| factor analysis of confocal image sequences of human papillomavirus dna revealed with fast red in cervical tissue sections stained with toto-iodide. | to visualize and localize specific dna sequences by fluorescence in situ hybridization, confocal laser scanning microscopy (clsm) and factor analysis of biomedical image sequences (famis). | 2000 | 10800619 |
| pkn binds and phosphorylates human papillomavirus e6 oncoprotein. | the high risk human papillomaviruses (hpvs) are associated with carcinomas of cervix and other genital tumors. previous studies have identified two viral oncoproteins e6 and e7, which are expressed in the majority of hpv-associated carcinomas. the ability of high risk hpv e6 protein to immortalize human mammary epithelial cells has provided a single gene model to study the mechanisms of e6-induced oncogenic transformation. in recent years, it has become clear that in addition to e6-induced degra ... | 2000 | 10809724 |
| human papillomavirus testing as a screening tool for cervical cancer | 2000 | 10815111 |