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impacts of antimalarial drugs on plasmodium falciparum drug resistance markers, western kenya, 2003-2015.antimalarial drug resistance has threatened global malaria control since chloroquine (cq)-resistant plasmodium falciparum emerged in asia in the 1950s. understanding the impacts of changing antimalarial drug policy on resistance is critical for resistance management. plasmodium falciparum isolates were collected from 2003 to 2015 in western kenya and analyzed for genetic markers associated with resistance to cq (pfcrt), sulfadoxine-pyrimethamine (sp) (pfdhfr/pfdhps), and artemether-lumefantrine ...201829363453
diarrhegenic escherichia coli replaces shigella sp. as the predominant bacteria causing childhood diarrhea in andaman and nicobar islands, india.the andaman and nicobar islands is an archipelago of more than 500 islands situated in the bay of bengal, about 1,200 km away from peninsular india (92° to 94°e longitude; 6° to 14°n latitude). the archipelago, a union territory of india, is inhabited by more than 350,000 people, including six aboriginal tribes and settlers from mainland india. acute diarrhea is common among the children of andaman islands.1,2 regional medical research center has been monitoring its clinical, epidemiological, an ...201829363445
the allometric scaling of body mass and height in children and adolescents in five countries.the purpose a methodology that excludes values outside of the expected spectrum for age (voesa) of height and body mass in the definition of power (p) to construct an international allometric body mass index (ai) for children and adolescents.201829363223
lower bone density, impaired microarchitecture, and strength predict future fragility fracture in postmenopausal women: 5-year follow-up of the calgary camos cohort.the aim of this prospective study was to use high-resolution peripheral quantitative computed tomography (hr-pqct) to determine if baseline skeletal parameters can predict incident fragility fracture in women and, secondly, to establish if women that fracture lose bone at a faster rate than those who do not fracture. women older than 60 years who experienced a fragility fracture during the 5-year follow-up period (incident fracture group, n = 22) were compared with those who did not experience a ...201829363165
a new species of petroleuciscus (teleostei: cyprinidae) from the büyük menderes river, southwestern anatolia, turkey.petroleuciscus ninae sp. nov. is described from the büyük menderes river drainage. the new species is distinguished by having a black lateral stripe from head to base of caudal fin, stripe distinct anteriorly and posteriorly, wider than eye diameter; numerous black pigments on anal-fin rays; body depth at dorsal-fin origin 27-30% standard length (ls ); head width at posterior margin of eye 16-19% ls ; and eye diameter smaller than snout length. petroleuciscus ninae is also distinguished from oth ...201829363130
combinatorial effect of abiraterone acetate and nvp-bez235 on prostate tumor progression in rats.use of drug combinations that target different pathways involved in the development and progression of prostate cancer (pca) has emerged as an alternative to overcome the resistance caused by drug monotherapies. the antiandrogen abiraterone acetate and the pi3k/akt inhibitor nvp-bez235 (bez235) may be suitable options for the prevention of drug resistance and the inhibition of pca progression. the aim of the present study was to evaluate whether abiraterone acetate and bez235 achieve superior th ...201829363091
ce-ms for the analysis of amino acids.amino acids play an important role in clinical analysis. capillary electrophoresis-electrospray ionization-mass spectrometry (ce-esi-ms) has proven to possess several characteristics that make it a powerful and useful tool for the analysis of amino acids in clinical studies. here we present a method for the separation and quantitative analysis of 27 amino acids in urine based on ce-esi-ms. the method presents an improved resolution between the isomers leu, ile, and aile, in comparison to other c ...201829363084
radiolabeled gx1 peptide for tumor angiogenesis imaging.early and accurate detection of primary or metastatic tumors is of great value in staging, treatment management, and prognosis. tumor angiogenesis plays an essential role in the growth, invasion, and metastatic spread of solid cancers, and so, is a promising approach for tumor imaging. the gx1 (cgnsnpksc) peptide was identified by phage display library and has been investigated as a marker for human cancers. this study aims to evaluate the 99mtc-hynic-peg4-c (gx1) as a biomarker for tumor imagin ...201829362989
"clothesline technique" for proximal tibial shaft fracture fixation using conventional intramedullary nail: a simple, useful, and inexpensive technique to prevent fracture malalignment.treatment of proximal tibial shaft fractures is always challenging. despite the development of modern techniques, the literature still shows high complication rates, especially regarding proximal fragment malalignment. it is well known that knee position in flexion during tibial nailing is responsible for extension and valgus deformities of the proximal fragment. unlike in tibial shaft fractures, nails do not reduce proximal tibial fractures due to the medullary canal width. this study aims to d ...201829362975
laser-assisted liposuction (lal) versus traditional liposuction: systematic review.liposuction is the most performed surgical procedure in brazil and the second in the world. in recent years, new technologies have been developed in an attempt to improve liposuction, such as laser. the objective of this study is to evaluate the efficacy and safety of laser-assisted liposuction (lal) compared to traditional liposuction through a systematic review of the literature.201829362943
cloning, expression, and biochemical characterization of two new oligoalginate lyases with synergistic degradation capability.alginate, the most abundant carbohydrate presents in brown macroalgae, has recently gained increasing attention as an alternative biomass for the production of biofuel. oligoalginate lyases catalyze the degradation of alginate oligomers into monomers, a prerequisite for bioethanol production. in this study, two new oligoalginate lyase genes, oalc6 and oalc17, were cloned from cellulophaga sp. sy116, and expressed them in escherichia coli. the deduced oligoalginate lyases, oalc6 and oalc17, belon ...201829362921
the ontogeny of the 300 million year old xiphosuran euproops danae (euchelicerata) and implications for resolving the euproops species complex.xiphosurans have often been considered as archaic appearing cheliceratan arthropods, with a rich fossil record. we describe here parts of the post-embryonic ontogeny of the 300 million year old xiphosuran euproops danae (xiphosura sensu stricto, euchelicerata), from the mazon creek lagerstätte (upper carboniferous), usa. recently, the ontogeny of a closely related species, euproops sp. from the upper carboniferous piesberg quarry, osnabrück, germany (informally called 'piesproops'), has been rec ...201829362894
spirosoma agri sp. nov., isolated from apple orchard soil.a gram-negative, non-motile, rod-shaped, aerobic bacterial strain, designated s7-3-3t, was isolated from apple orchard soil in gyeongsangnam-do province, south korea, and was characterized taxonomically using a polyphasic approach. phylogenetic analysis based on 16s rrna gene sequence showed that strain s7-3-3t belonged to the family cytophagaceae in the phylum bacteroidetes was most closely related to spirosoma rigui wpcb118t (94.3%), spirosoma pulveris jsh5-14t (93.9%), and spirosoma linguale ...201829362880
zornia latifolia: a smart drug being adulterated by stylosanthes guianensis.dried herbal preparations, based on "zornia latifolia," are commonly sold on web, mainly for their supposed hallucinogenic properties. in this work, we demonstrate that these commercial products contain a different fabacea, i.e., stylosanthes guianensis, a cheaper plant, widely cultivated in tropical regions as a fodder legume. we were provided with plant samples of true zornia latifolia from brazil, and carried out a thorough comparison of the two species. the assignment of commercial samples w ...201829362872
assessment of turkey vehicle container microclimate on transit during summer season conditions.this study evaluated the formed microclimate commercial truck transport practices effects on the turkeys' welfare by measuring dead on arrival (doa) index and pale, soft, and exudative (pse-like) meat occurrence. the experimental design was entirely randomized in a 6 × 2 factorial arrangements (two truck container compartments × six water shower groups) with birds positioned at superior front (sf), inferior front (if), superior middle (sm), inferior middle (im), superior rear (sr), and inferior ...201829362871
ligictaluridus michaelalicea n. sp. (monogenea: dactylogyridae) from flathead catfish (pylodictis olivaris) in the upper mississippi river, including remarks on taxonomy influencing monogenean treatment regulation in the united states.ligictaluridus michaelalicea n. sp. (monogenea: dactylogyridae, ancyrocephalinae) is described from the gills of pylodictis olivaris (siluriformes: ictaluridae) from wisconsin and iowa portions of the upper mississippi river. diagnostic features include a relatively large, strongly curved tubular cirrus with minor terminal flare; an accessory piece with a prominent basal lobe and a simple, thick terminal limb featuring a thin lateral flange and blunt distal tip devoid of recurved hooks. the sini ...201829362870
phylogenetic relationships and genetic diversity of the polypedates leucomystax complex in thailand.taxonomic uncertainty of the asian tree frog polypedates leucomystax complex presents the challenging task of inferring its biogeographical history. here, we describe its dispersion and the genetic relationships among different populations in thailand, where we connect the population of the p. leucomystax complex of the sunda islands to the indochina (mainland) population based on analyses of 266 sequences of the mitochondrial cytochrome c oxidase subunit i (coi) gene. our maternal genealogy imp ...201829362696
studies in two allopatric populations of hypostomus affinis (steindachner, 1877): the role of mapping the ribosomal genes to understand the chromosome evolution of the group.several cytogenetic markers show chromosomal diversity in the fish such as "armoured catfish". although studies have characterized many species in the major genera representing these siluridae, particularly in the genus hypostomus lacépède, 1803, trends in chromosome evolution of this group remain unclear. the paraíba do sul river basin contains the armoured catfish hypostomus affinis steindachner, 1877, which is unique because of its distribution of repetitive dnas, the 5s and 18s rdna. identif ...201829362669
two new species of lobellini from tianmu mountain, china (collembola, neanuridae).three species of the subfamily neanurinae (collembola: neanuridae) are recorded from tianmu mountain, zhejiang province, east china. two of them, lobellina fusasp. n. and paralobella tianmunasp. n., are new to science and described in this paper. lobellina fusasp. n. can be recognized by the presence of six teeth on mandible and the fusion of dorsointernal tubercles on the head. paralobella tianmunasp. n. is characterized by a mandible with seven teeth, the lateral tubercle of abd. ii-iii respec ...201829362544
cathepsin d immobilized capillary reactors for on-flow screening assays.the treatment of diseases using enzymes as targets has called for the development of new and reliable methods for screening. the protease cathepsin d is one such target involved in several diseases such as tumors, degenerative processes, and vital processes of parasites causing schistosomiasis. herein, we describe the preparation of a fused silica capillary, cathepsin d (catd)-immobilized enzyme reactor (imer) using in a multidimensional high performance liquid chromatography-based method (2d-hp ...201829367161
improved heterologous expression of the membrane-bound quinoprotein quinate dehydrogenase from gluconobacter oxydans.gluconobacter oxydans produces 3-dehydroquinate by oxidation of quinate through a reaction catalyzed by the quinate dehydrogenase (qdh), membrane-bound, pyrroloquinoline quinone (pqq)-dependent dehydrogenase. we previously reported the nucleotide and deduced amino acid sequence of qdh and constructed a heterologous expression system of qdh in pseudomonas sp. (a.s. vangnai, w. promden, w. de-eknamkul, k. matsushita, h. toyama, biochemistry (moscow) 75:452-459, 2010). through this study, we aim to ...201829366965
evaluation of toxicity of glycerol monooleate nanoparticles on pc12 cell line.an innovative approach to improve drug delivery is the use of glycerol monooleate nanoparticles. numerous studies describe their high versatility, low toxicity and ability to carry relatively high loads of conjugated compounds including scarcely soluble ones, providing sustained drug release and increasing drug diffusion and half-life. despite a growing interest in their potential use for therapeutic applications, there are surprisingly few literature data concerning the toxic effects of these n ...201829366940
a low-molecular-weight galactofucan from the seaweed, spatoglossum schröederi, binds fibronectin and inhibits capillary-like tube formation in vitro.a low-molecular-weight (lmw) heterofucan (designated fucan b) was obtained from the brown seaweed, spatoglossum schröederi, and its activity as an inhibitor of capillary-like tube formation by endothelial cells (ecs) was analyzed. chemical, infrared and electrophoretic analyses confirmed the identity of fucan b. in contrast to other lmw fucans, fucan b (0.012-0.1 mg/ml) inhibited ecs capillary-like tube formation in a concentration-dependent manner. in addition, fucan b (0.01-0.05 mg/ml) did not ...201829366897
extraction and characterization of chitosan from prawn shell waste and its conjugation with cutinase for enhanced thermo-stability.the present article describes extraction of chitosan from prawn shells waste and its application in thermal stabilization of fusarium sp. ict sac1 cutinase by non-covalent and covalent conjugation. extracted chitosan represented 78.40% degree of deacetylation (dda), a molecular weight of 173 kda and was soluble in 1% acetic acid with 2.8 ± 0.15% insoluble matter. the structural (ftir, nmr and xrd) and thermal characterization (dsc and tga) indicated unique properties for chitosan. plausible chit ...201829366886
α- and β-d-glucans from the edible mushroom pleurotus albidus differentially regulate lipid-induced inflammation and foam cell formation in human macrophage-like thp-1 cells.macrophages play an essential role in lipid metabolism; however, the excessive uptake of modified lipids and cholesterol crystals (cc) leads to the formation of pro-inflammatory lipid-laden macrophages called foam cells. since the α-1,6- and β-1,3-d-glucans from the basidiome and the mycelium of the edible mushroom pleurotus albidus have previously been shown to regulate macrophage function, these glucans were tested in macrophage-like thp-1 cells previously exposed to acetylated low-density lip ...201829366884
in vivo assessment of the hepatotoxicity of a new nostoc isolate from the nile river: nostoc sp. strain nri.nostoc sp. is one of the most widely distributed cyanobacterial genera that produce potentially protein phosphatase (pp) inhibitor; microcystins (mcs). mcs have posed a worldwide concern due to predominant hepatotoxicity to human health. we have previously isolated a nostoc strain (nr1) from the nile river (the main water supply in egypt) and this strain exerted production of rare and highly toxic mc; demethylated microcystin-lr. there is no data concerning risk factors of liver diseases for hum ...201829366868
indole diterpenoids from the endophytic fungus drechmeria sp. as natural antimicrobial agents.a fungal strain, drechmeria sp., was isolated from the root of panax notoginseng. totally, seven new indole diterpenoids, drechmerins a-g (1-7), were isolated from the fermentation broth of drechmeria sp. together with four known analogues (8-11). their structures were determined on the basis of 1d and 2d nmr and electronic circular dichroism (ecd) spectroscopic analyses as well as theoretical calculations. all the isolated compounds were evaluated for their antimicrobial activities against cand ...201829366852
electrical stimulation or mk-801 in the inferior colliculus improve motor deficits in mptp-treated mice.the inferior colliculus (ic) is an important midbrain relay station for the integration of descending and ascending auditory information. additionally, the ic has been implicated in processing sensorimotor responses. glutamatergic and gabaergic manipulations in the ic can improve motor deficits as demonstrated by the animal model of haloperidol-induced catalepsy. however, how the ic influences motor function remains unclear. we investigated the effects of either intracollicular deep brain stimul ...201829366825
on the quantitative phase analysis and amorphous content of triacylglycerols materials by x-ray rietveld method.the characterization of fat components becomes very useful for formulation of shortening, margarines and fat products due to their unique properties of plasticity, texture, solubility, and aeration. however, x-ray diffraction experiments on such materials are usually limited to a qualitative evaluation of the polymorphic properties based only on the characteristic d-spacing peak intensities. in this work, interesting results based on the rietveld method have supported both a quantitative phase a ...201829366737
[a case of sigmoid colon cancer it was found from penetration of sigmoid diverticulum].a 52-year-old woman presented with sudden left lower abdominal pain, fever, and vomiting. as the symptom got improved immediately she went home then. she consulted our hospital with chief complaint of the left lower abdominal mass. abdominal computed tomography revealed sigmoid colon cancer with abscess. she was diagnosed with sigmoid colon cancer via colonoscopy. sigmoidectomy including partial resection of the abdominal wall was performed(d3 lymphadenectomy). surgical specimen showed penetrati ...201829362329
protocol for the systematic review of the reporting of transoral robotic surgery.transoral robotic surgery (tors) has been adopted in some parts of the world as an innovative approach to the resection of oropharyngeal tumours. the development, details and outcomes of early-to-later phase evaluation of this technique and the quality of evidence to support its adoption into practice have hitherto not been summarised. the aim of this review is to identify and summarise the early and later phase studies of, and evidence for, tors and to understand how early phase studies report ...201829362265
lesser metatarsophalangeal joint instability: treatment with tendon transfers.complex digital deformities and metatarsophalangeal joint instability encompass a wide range of pathology, and we must identify the different degrees of ligamentous disruption. it is important to address a combination of procedures to treat gross deformities of the lesser toes. surgical treatment should be individualized and requires a sequential process for adequate reduction and deformity correction. there is no gold standard procedure for every deformity. although residual stiffness can resul ...201829362027
gastroprotective effects of sulphated polysaccharides from the alga caulerpa mexicana reducing ethanol-induced gastric damage.the development of the gastric lesion is complex and the result of the imbalance between aggressive and protective factors, involving the generation of free radicals and disturbance in nitric oxide (no) production. sulphated polysaccharides (sp), from marine algae, are widely used in biotechnological and pharmaceutical areas. in this study, we evaluated the effects of sp from the green marine alga caulerpa mexicana (cm-sp) in ethanol-induced gastric damage models in mice. cm-sp (2, 20, or 200 mg ...201829361672
corrigendum to "phosphorylated-tyrosine based pseudobioaffinity adsorbent for the purification of immunoglobulin g" [j. chromatogr. b, 1052 (2017) 10-18]. 201829366585
obesity and hypertension in latin america: current perspectives.in the countries of central america, south america and the caribbean, there has been a dramatic rise in obesity, the metabolic syndrome, hypertension and other cardiovascular risk factors in the last few decades. epidemiological evidence highlights a consistent correlation between obesity and hypertension, and the presence of obesity predisposes an individual to a greater risk of hypertension although the mechanisms remain unclear. obesity and hypertension are two key drivers of the cardio-renal ...201829361428
exploration of c-h transformations of aldehyde hydrazones: radical strategies and beyond.the chemistry of hydrazones has gained great momentum due to their involvement throughout the evolution of organic synthesis. herein, we discuss the tremendous developments in both the methodology and application of hydrazones. hydrazones can be recognized not only as synthetic equivalents to aldehydes and ketones but also as versatile synthetic building blocks. consequently, they can participate in a range of practical synthetic transformations. furthermore, hydrazone derivatives display a broa ...201829359909
2de gels: a story of love and hate in proteomics.i think 2de may still be a very useful proteomic tool. in 2014, we published an article defending the usefulness of 2de gels in proteomic research [1]. a little earlier, roepstorff´s group did the same [2].201829359858
generating plasmonic heterostructures by cation exchange and redox reactions of covellite cus nanocrystals with au3+ ions.we demonstrate the fabrication of various types of heterostructures, including core-shells and dimers. this is achieved by reacting platelet-shaped covellite (cus) nanocrystals (ncs) with au3+ ions under various reaction conditions: the exposure of cus ncs to au3+ ions, in the presence or in the absence of ascorbic acid (aa), leads to the formation of cus@au core-shell nanostructures; the reaction of cus ncs with au3+ ions in the presence of oleylamine (om) leads to the formation of cus@au2s; th ...201829359781
cleavage activates dispatched for sonic hedgehog ligand release.hedgehog ligands activate an evolutionarily conserved signaling pathway that provides instructional cues during tissue morphogenesis, and when corrupted, contributes to developmental disorders and cancer. the transmembrane protein dispatched is an essential component of the machinery that deploys hedgehog family ligands from producing cells, and is absolutely required for signaling to long-range targets. despite this crucial role, regulatory mechanisms controlling dispatched activity remain larg ...201829359685
synthesis of polyynes using dicobalt masking groups.extended triisopropylsilyl end-capped polyynes have been prepared from the corresponding tetracobalt complexes by removing the complexed dicobalt tetracarbonyldiphenylphosphinomethane (co2(co)4dppm) moieties. unmasking of this 'masked alkyne equivalent' was achieved under mild conditions with elemental iodine at room temperature, making it possible to obtain fragile polyynes with up to 20 contiguous sp-hybridized carbon atoms. the co2(co)4dppm moiety has a strong geometric and steric effect on t ...201829359550
rhodium(i)-catalyzed cycloisomerization of homopropargylallene-alkynes through c(sp3)-c(sp) bond activation.upon exposure to a catalytic amount of [rhcl(co)2]2 in 1,4-dioxane, homopropargylallene-alkynes effected a novel cycloisomerization accompanied by the migration of alkyne moiety of its homopropargyl functionality to produce the six/five/five tricyclic compounds in good yields. the plausible mechanism could be proposed on the basis of the experiment using the 13c-labeled substrate. the resulting tricyclic derivatives were further converted into the corresponding bicyclo[3.3.0] skeleton with vicin ...201829359501
the spleen of patients with myelofibrosis harbors defective mesenchymal stromal cells.splenic hematopoiesis is a major feature in the course of myelofibrosis (mf). in fact, the spleen of patients with mf contains malignant hematopoietic stem cells retaining a complete differentiation program, suggesting both a pivotal role of the spleen in maintaining the disease and a tight regulation of hematopoiesis by the splenic microenvironment, in particular by mesenchymal stromal cells (mscs). little is known about splenic mscs (sp-mscs), both in normal and in pathological context. in thi ...201829359451
previous exposure to chronic intermittent hypoxia blunts the development of one-kidney one-clip hypertension in rats.chronic intermittent hypoxia (cih) and one-kidney one-clip (1k, 1c) experimental models lead to sympathetic overactivity and hypertension. we hypothesized that previous exposure to cih potentiates the development of 1k, 1c renal hypertension. male rats were divided into 4 groups: control-1k, 1c, maintained under normoxia followed by 1k, 1c surgery (n = 19); control-sham, maintained under normoxia, followed by sham surgery (n = 19); cih-1k, 1c, exposed to cih (10 days) and 1k, 1c surgery (n = 19) ...201829359403
heterologous expression of oenococcus oeni shsp20 confers temperature stress tolerance in escherichia coli.small heat shock proteins (shsps) are heat shock proteins sized 12-43 kda that can protect proteins from denaturation, particularly under high temperature; shsps thus increase the heat tolerance capability of an organisms enabling survival in adverse climates. shsp20 is overexpressed in oenococcus oeni in response to low temperatures. however, we found that overexpression of shsp20 in escherichia coli bl21 increased the microbial survival ratio at 50 °c by almost 2 h. adding shsp20 to the glutam ...201829359265
nano-silver modifies the vase life of cut herbaceous peony (paeonia lactiflora pall.) flowers.herbaceous peony (paeonia lactiflora pall.) is a popular high-grade cut flower because of higher ornamental value. however, its short flowering time severely restricts the production and application of cut p. lactiflora flowers. in this study, nano-silver (ns) was applied to prolong the vase life of cut p. lactiflora flowers. under the ns treatment, related physiological indices including relative electrical conductivity (rec), malondialdehyde (mda), superoxide anion free radical (o2·-), hydroge ...201829359232
capillary electrophoresis to approach sorbate usage in processed meat products in brazil.sorbate is a highly used preservative in a wide range of processed foods, including meat products. in this work sorbate usage in commercial processed meat products from the brazilian market was evaluated. a capillary electrophoresis method for sorbate analysis using ethanol:water extraction solution (1:2, v/v) and sodium tetraborate (20 mmol l-1) electrolyte solution was validated. low limits of detection (0.4 mg l-1) and quantification (1.0 mg l-1), good precision (rsd = 3.6%) and suitable accu ...201829358839
characterization of baru nut (dipteryx alata vog) flour and its application in reduced-fat cupcakes.baru is a native specie from the brazilian "cerrado" with interesting nutritional and sensory characteristics. the aim of our study was to characterize baru nut flour (bf) and to explore the possibility of producing reduced-fat baru cupcakes. four different cupcake formulations were produced wheat flour (wf) containing 30% bf with reductions of 50 (f1), 75 (f2) and 100% (f3) margarine, compared to a control with 100% wf and 100% margarine (fc). bf showed 2.76% moisture, 19.2% proteins, 40.8% lip ...201829358807
impact of pre-drying and frying time on physical properties and sensorial acceptability of fried potato chips.in this work the effects of pre-drying and frying time on colour, oil, texture and sensorial acceptability (overall liking) of potato chips were evaluated. potato chips were pre-dried for 0, 10, 20 and 30 min at 60 °c and fried in soybean oil at 190 °c for 60, 70 and 80 s. the colour parameters (l*, a* and b*) increased or decreased depending on the pre-drying and frying time. hardness increased as the pre-drying and frying time increased. on the other hand, the water initially removed by pre-dr ...201829358804
assessment of chemical and sensory quality of sugarcane alcoholic fermented beverage.this study aimed to verify the technological feasibility, chemical quality and sensory acceptance of alcoholic fermented beverage obtained from sugarcane juice. a completely randomized design was applied. sugar and alcohol content, phenolic (hplc-ms) and volatile (gs-ms) compounds, ph, density, dry matter and acidity of the fermented beverage of sugarcane were quantified, as well as the acceptance of the product was carried out. the complete fermentation of sugarcane lasted 7 days, and it was ob ...201829358797
kinin-b1 receptor stimulation promotes invasion and is involved in cell-cell interaction of co-cultured glioblastoma and mesenchymal stem cells.glioblastoma multiforme (gbm) represents the most lethal brain tumour, and these tumours have very limited treatment options. mesenchymal stem cells (msc) are considered as candidates for advanced cell therapies, due to their tropism towards gbm, possibly affecting their malignancy, thus also representing a potential therapeutic vector. therefore, we aimed to compare the effects of bone-marrow-derived versus adipose-tissue-derived msc (bm-/at-msc) on heterogeneous populations of tumour cells. th ...201829358738
network of proteins, enzymes and genes linked to biomass degradation shared by trichoderma species.understanding relationships between genes responsible for enzymatic hydrolysis of cellulose and synergistic reactions is fundamental for improving biomass biodegradation technologies. to reveal synergistic reactions, the transcriptome, exoproteome, and enzymatic activities of extracts from trichoderma harzianum, trichoderma reesei and trichoderma atroviride under biodegradation conditions were examined. this work revealed co-regulatory networks across carbohydrate-active enzyme (cazy) genes and ...201829358662
early hominids may have been weed species.panid, gorillid, and hominid social structures appear to have diverged as dramatically as did their locomotor patterns as they emerged from a late miocene last common ancestor (lca). despite their elimination of the sectorial canine complex and adoption of bipedality with its attendant removal of their ready access to the arboreal canopy, australopithecus was able to easily invade novel habitats after florescence from its likely ancestral genus, ardipithecus sp. other hominoids, unable to sustai ...201829358388
how proteolytic inhibitors interact with dentin on glass-fiber post luting over 6 months.enzyme inhibitors minimize the degradation of unprotected collagen of dentin promoted by matrix metalloproteinases (mmps) and cysteine cathepsins (ccs). as the evidence of their effect on the root canal is limited, this study aimed to evaluate the role of edta, chlorhexidine and e-64 as antiproteolytic agents on the bond strength (bs) of glass-fiber posts in root canals.201829358152
long term exposure to low dose neurotoxic pesticides affects hatching, viability and cholinesterase activity of artemia sp.the brine shrimp artemia was used as a model organism to test toxicity of several neuroactive pesticides (chlorpyrifos (clp), chlorpyrifos oxon (clp ox), diazinon (dzn), carbaryl (cbr)) following exposure to far below than lethal doses. cysts were exposed to the pesticides in order to test a scenario similar to actual coastal environment contamination, by analyzing different responses. cysts were rehydrated in water containing the pesticides at concentrations ranging from 10-11 to 10-5 m, for 72 ...201829358113
can artificial tears prevent acanthamoeba keratitis? an in vitro approach.the use of contact lenses has increased in recent years as has the incidence of dry eye syndrome, partly due to their use. artificial tears are the most common treatment option. since these changes can facilitate acanthamoeba infection, the present study has been designed to evaluate the effect of three artificial tears treatments in the viability of acanthamoeba genotype t4 trophozoites. optava fusion™, oculotect®, and artelac® splash were selected due to their formulation.201829357901
fam3b/pander inhibits cell death and increases prostate tumor growth by modulating the expression of bcl-2 and bcl-xl cell survival genes.fam3b/pander is a novel cytokine-like protein that induces apoptosis in insulin-secreting beta-cells. since in silico data revealed that fam3b can be expressed in prostate tumors, we evaluated the putative role of this cytokine in prostate tumor progression.201829357840
interfering rna against pkc-α inhibits tnf-α-induced ip3r1 expression and improves glomerular filtration rate in rats with fulminant hepatic failure.we have reported that tumor necrosis factor- (tnf-α) is critical for reduction of glomerular filtration rate (gfr) in rats with fulminant hepatic failure (fhf). the present study aims to evaluate the underlying mechanisms of decreased gfr during acute hepatic failure.201829357415
pulsed radiofrequency improves neuropathic pain in chronic constriction injury rats through the upregulation of the transcription and translation levels of glial cell line-derived neurotrophic factor.several studies in recent years have confirmed that the direct application of pulsed radiofrequency (prf) on peripheral nerve compression points can alleviate hyperalgesia in animal models of neuropathic pain (np). however, the mechanism underlying the treatment of peripheral nerves by prf is unclear.201829357329
magnetic resonance perfusion for differentiating low-grade from high-grade gliomas at first presentation.gliomas are the most common primary brain tumour. they are graded using the who classification system, with grade ii-iv astrocytomas, oligodendrogliomas and oligoastrocytomas. low-grade gliomas (lggs) are who grade ii infiltrative brain tumours that typically appear solid and non-enhancing on magnetic resonance imaging (mri) scans. people with lgg often have little or no neurologic deficit, so may opt for a watch-and-wait-approach over surgical resection, radiotherapy or both, as surgery can res ...201829357120
a new genus and species of turtle blood fluke (digenea: schistosomatoidea) from the mekong snail-eating turtle, malayemys subtrijuga (schlegel & müller) (testudines: geoemydidae) in vietnam, with a reassessment of related asiatic turtle blood flukes and molecular phylogeny.platt sinuosus roberts & bullard n. g., n. sp. (type-species) infects the kidney and mesenteric blood vessels of mekong snail-eating turtles, malayemys subtrijuga (schlegel & müller), in the mekong river basin. species of platt roberts & bullard n. g. are unique by the combination of having a papillate ventral sucker, vasa efferentia that are dorsal to the gonads, a massive cirrus-sac that is directed anteriad or laterad, and a vitellarium that surrounds the intestinal caeca. the new species res ...201829357101
chemical profile of the sulphated saponins from the starfish luidia senegalensis collected as by-catch fauna in brazilian coast.the by-catch fauna of the shrimp fishery includes a number of marine invertebrates that are discarded because they do not have commercial value. in order to try to add some value to these materials, we analyzed the chemical composition of the starfish luidia senegalensis collected in the brazilian coast as a consequence of the trawling fishery method. in order to access their chemical composition, we used a combination of solid phase extraction (spe) followed by ultra-high performance liquid chr ...201829357091
possible breathing influences on the control of arterial pressure after sino-aortic denervation in rats.surgical removal of the baroreceptor afferents [sino-aortic denervation (sad)] leads to a lack of inhibitory feedback to sympathetic outflow, which in turn is expected to result in a large increase in mean arterial pressure (map). however, few days after surgery, the sympathetic nerve activity (sna) and map of sad rats return to a range similar to that observed in control rats. in this review, we present experimental evidence suggesting that breathing contributes to control of sna and map follow ...201829356918
enhancement of schizochytrium dha synthesis by plasma mutagenesis aided with malonic acid and zeocin screening.schizochytrium sp. accumulates valuable polyunsaturated fatty acid (pufa), such as docosahexaenoic acid (dha). in order to increase dha synthesis in this microorganism, physical or chemical mutagenesis aided with powerful screening methods are still preferable, as its dha synthetic pathway has not yet been clearly defined for gene manipulation. to breed this agglomerate microorganism of thick cell wall and rather large genome for increasing lipid content and dha percentage, a novel strategy of a ...201829356868
limoniibacter endophyticus gen. nov., sp. nov., an alphaproteobacterium isolated from the roots of limonium otolepis.a gram-negative bacterium, designated as strain yim 690229t, was isolated from the roots of limonium otolepis. the strain was able to grow at 10-40 °c (optimum, 28-37 °c), ph 6.0-8.0 (optimum, 7.0) and in the presence of up to 7% nacl (w/v) (optimum, up to 2.5%). comparative 16s rrna gene sequence analysis revealed that strain yim 690229t shared less than 93.9% sequence similarities with members within the order rhizobiales, and was remotely related to members of the family hyphomicrobiaceae. st ...201829356837
a novel family of tyrosine integrases encoded by the temperate pleolipovirus snj2.genomes of halophilic archaea typically contain multiple loci of integrated mobile genetic elements (mges). despite the abundance of these elements, however, mechanisms underlying their site-specific integration and excision have not been investigated. here, we identified and characterized a novel recombination system encoded by the temperate pleolipovirus snj2, which infects haloarchaeon natrinema sp. j7-1. snj2 genome is inserted into the trnamet gene and flanked by 14 bp direct repeats corres ...201829361162
a new species of simulium (asiosimulium) (diptera: simuliidae) from thailand.simulium (asiosimulium) saeungae sp. nov. (diptera: simuliidae) is described based on females, males, pupae, and mature larvae collected from nan province, northern thailand. it is characterized by the medium-long cerci in the female, enlarged hind basitarsus, and broad ventral plate with its posterior margin not deeply concave in the male, arborescent pupal gill with 42-56 filaments in the pupa and smaller number of primary rays of the labral fan (30-33) in the larva. this is the fifth species ...201829361148
a new species of the simulium (simulium) striatum species group (diptera: simuliidae) from thailand, and its differentiation from two related species based on a fast-evolving nuclear gene.simulium (simulium) phraense sp. nov. (diptera: simuliidae) is described from females, males, pupae, and larvae from thailand. this new species is placed in the simulium striatum species group and is most similar to simulium (simulium) nakhonense takaoka & suzuki (diptera: simuliidae) from thailand among species of the same species group but is barely distinguished from the latter species by lacking annular ridges on the surface of the pupal gill filaments. the fast-evolving nuclear big zinc fin ...201829361011
abc transporters of the multicellular cyanobacterium anabaena sp. pcc 7120: a wide variety for a complex lifestyle.two hundred genes or 3% of the known or putative protein-coding genes of the filamentous freshwater cyanobacterium anabaena sp. pcc 7120 encode domains of atp-binding cassette (abc) transporters. detailed characterization of some of these transporters (14 - 15 importers and 5 exporters) has revealed their crucial roles in the complex lifestyle of this multicellular photoautotroph, which is able to differentiate specialized cells for nitrogen fixation. this review summarizes the characteristics o ...201829360977
transcriptional regulation of the mannan utilization genes in the alkaliphilic bacillus sp. n16-5.bacillus sp. n16-5 is an alkaliphile with great ability of utilizing mannan. its mannan utilization gene cluster has been identified in a previous study. the manr protein encoded by the cluster was predicted to be a laci family regulator, and the transcription level of the mannan utilization gene cluster was upregulated after the manr gene was deleted, indicating that manr is the repressor of this cluster. the transcription of the related genes was downregulated when manh, encoding the extracell ...201829360976
community structure of photosynthetic picoeukaryotes differs in lakes with different trophic statuses along the middle-lower reaches of the yangtze river.photosynthetic picoeukaryotes (ppes) play an important role in aquatic ecosystem functioning. there is still a relative lack of information on freshwater ppes, especially in eutrophic lakes. we used a combination of flow cytometric sorting and pyrosequencing to investigate the ppes community structure in more than 20 mesotrophic and eutrophic lakes along the middle-lower reaches of the yangtze river in china. the abundance of ppes ranged between 2.04 × 103 cells ml-1 and 5.92 × 103 cells ml-1. t ...201829360960
mucosal immunization with pspa (pneumococcal surface protein a)-adsorbed nanoparticles targeting the lungs for protection against pneumococcal infection.burden of pneumonia caused by streptococcus pneumoniae remains high despite the availability of conjugate vaccines. mucosal immunization targeting the lungs is an attractive alternative for the induction of local immune responses to improve protection against pneumonia. our group had previously described the development of poly(glycerol adipate-co-ω-pentadecalactone) (pga-co-pdl) polymeric nanoparticles (nps) adsorbed with pneumococcal surface protein a from clade 4 (pspa4pro) within l-leucine m ...201829360883
mid-term sustained relief from headaches after balloon angioplasty of the internal jugular veins in patients with multiple sclerosis.multiple sclerosis (ms) patients frequently suffer from headaches and fatigue, and many reports have linked headaches with intracranial and/or extracranial venous obstruction. we therefore designed a study involving ms patients diagnosed with obstructive disease of internal jugular veins (ijvs), with the aim of evaluating the impact of percutaneous transluminal angioplasty (pta) on headache and fatigue indicators.201829360844
zoanthid mucus as new source of useful biologically active proteins.palythoa caribaeorum is a very common colonial zoanthid in the coastal reefs of brazil. it is known for its massive production of mucus, which is traditionally used in folk medicine by fishermen in northeastern brazil. this study identified biologically active compounds in p. caribaerum mucus. crude mucus was collected during low tides by the manual scraping of colonies; samples were maintained in an ice bath, homogenized, and centrifuged at 16,000 g for 1 h at 4 °c; the supernatant (mucus) was ...201829360533
idna6ma-pseknc: identifying dna n6-methyladenosine sites by incorporating nucleotide physicochemical properties into pseknc.n6-methyladenine (6ma) is one kind of post-replication modification (ptm or ptrm) occurring in a wide range of dna sequences. accurate identification of its sites will be very helpful for revealing the biological functions of 6ma, but it is time-consuming and expensive to determine them by experiments alone. unfortunately, so far, no bioinformatics tool is available to do so. to fill in such an empty area, we have proposed a novel predictor called idna6ma-pseknc that is established by incorporat ...201829360500
ultrastructural analysis of apicomplexa-like parasites in two conch species laevistrombus canarium and canarium urceus from johor straits, malaysia.the tropical conch, laevistrombus canarium (linnaeus, 1758) and canarium urceus (linneaus, 1758) are ecologically and economically important shellfish species in malaysia and neighboring region. their populations, however are currently declining and this histopathological study investigates the aspect of parasitism and diseases that may affect their well-being. conch samples were randomly collected from their natural habitat and histological sections (4 - 5 µm) of various organs and tissues were ...201829360442
a comprehensive survey of aeromonas spp. and vibrio spp. in seabirds from southeastern brazil: outcomes for public health.to perform a microbiological survey regarding the presence, prevalence and characterization of aeromonas sp. and vibrio sp. in debilitated wrecked marine birds recovered from the center-north coast of the state of rio de janeiro, brazil.201829356247
tumor necrosis factor-α regulates human follicular dendritic cell-secreted protein gene transcription in gingival epithelial cells.follicular dendritic cell-secreted protein (fdc-sp) is a secreted protein expressed in follicular dendritic cells, periodontal ligament and junctional epithelium. to elucidate the transcriptional regulation of the human fdc-sp gene by tumor necrosis factor-α (tnf-α), we conducted real-time pcr, western blotting, transient transfection analyses with chimeric constructs of the fdc-sp gene promoter linked to a luciferase reporter gene, gel mobility shift and chromatin immunoprecipitation assays usi ...201829356241
curcuma sp.-derived dehydrocurdione induces heme oxygenase-1 through a michael reaction between its α, β-unsaturated carbonyl and keap1.to elucidate the anti-inflammatory mechanism of curcuma sp., we investigated whether dehydrocurdione, a sesquiterpene contained in curcuma sp., induces heme oxygenase (ho)-1, an antioxidative enzyme, in raw 264.7 macrophages. dehydrocurdione was extracted from the rhizome of curcuma sp., and its purity was verified by high performance liquid chromatography. treatment with 10-100 μm dehydrocurdione transiently and concentration-dependently increased ho-1 mrna and protein levels. docking simulatio ...201829356228
carbonic anhydrase ii does not exhibit nitrate reductase or nitrous anhydrase activity.carbonic anhydrase ii (ca ii) is a zinc metalloenzyme that catalyzes the reversible interconversion of water and co2 to bicarbonate and a proton. ca ii is abundant in most cells, and plays a role in numerous processes including gas exchange, epithelial ion transport, respiration, extra- and intracellular ph control, and vascular regulation. beyond these co2 and ph-linked roles, it has been postulated that ca ii might also reduce nitrite (no2-) to nitric oxide (no), as bicarbonate and no2- both e ...201829355738
new record of ascaridia nymphii (secernentea: ascaridiidae) from macaw parrot, ara chloroptera, in china.present study was performed to identify the species of ascarids from macaw parrot, ara chloroptera, in china. total 6 ascarids (3 males and 3 females) were collected in the feces of 3 macaws at guangzhou zoo in guangdong province, china. their morphological characteristics with dimensions were observed under a light microscope, and their genetic characters were analyzed with the partial 18s rdna, its rdna and nad4 gene sequences, respectively. results showed that all worms have no interlabia but ...201829355612
dietary patterns associated with overweight among brazilian adolescents.the present study aims to identify the dietary patterns of adolescents and associate these patterns with overweight. we analyzed food-consumption data from 6784 adolescents in the age group 10-18 years old collected in the household budget survey 2008-2009. dietary patterns were assessed through exploratory factor analysis. logistic regression models were used in order to associate dietary patterns with overweight. four dietary patterns were recorded: traditional brazilian pattern, snacks patter ...201829355584
piperlongumine and some of its analogs inhibit selectively the human immunoproteasome over the constitutive proteasome.the natural small molecule piperlongumine a is toxic selectively to cancer cells in vitro and in vivo. this toxicity has been correlated with cancer cell ros, dna damage and apoptotic cell death increases. we demonstrate here a new mechanistic property of piperlongumine: it inhibits selectively human immunoproteasome with no noticeable inhibition of human constitutive proteasome. this result suggests that immunoproteasome inhibition, a mechanism independent of ros elevation, may also partly play ...201829355526
diagnostic accuracy of the veteran affairs' traumatic brain injury screen.to comprehensively estimate the diagnostic accuracy and reliability of the department of veterans affairs (va) tbi clinical reminder screen (tcrs).201829355506
on the trails of the proteasome fold: structural and functional analysis of the ancestral β-subunit protein anbu.the 20s proteasome is a key player in eukaryotic and archaeal protein degradation, but its progenitor in eubacteria is unknown. recently, the ancestral β-subunit protein (anbu) was predicted to be the evolutionary precursor of the proteasome. we crystallised anbu from hyphomicrobium sp. strain mc1 in four different space groups and solved the structures by sad-phasing and patterson search calculation techniques. our data reveal that anbu adopts the classical fold of ntn-hydrolases, but its oligo ...201829355501
adamts-1 disrupts hgf/c-met signaling and hgf-stimulated cellular processes in fibrosarcoma.extracellular matrix (ecm) serves as a reservoir for biologically active factors, such as growth factors and proteases that influence the tumor cell behavior. adamts-1 (a disintegrin and metalloprotease with thrombospondin motifs) is a secreted protease that has the ability to modify the ecm during physiological and pathological processes. here, we analyzed the role played by adamts-1 regulating hgf and tgf-β1 activities in the high-grade fibrosarcoma cell line (ht1080). we generated ht1080 and ...201829355494
slowly progressive behavioral frontotemporal dementia with c9orf72 mutation. case report and review of the literature.we present a 86-year-old woman without relevant medical history and two brothers who died by dementia, who started at 55 years with depression and personality changes with ongoing worsening (>30 years) and functional decline. screening dementia blood test and brain magnetic resonance imaging did not show results that pointed to a secondary cause. the patient met the diagnostic criteria for possible behavioral frontotemporal dementia with a slow progression (bvftd-sp), suggesting a benign variant ...201829355451
insulin sensitivity index-based optimization of insulin to carbohydrate ratio: in silico study shows efficacious protection against hypoglycemic events caused by suboptimal therapy.the insulin to carbohydrate ratio (cr) is a parameter used by patients with type 1 diabetes (t1d) to calculate the premeal insulin bolus. usually, it is estimated by the physician based on patient diary, but modern diabetes technologies, such as subcutaneous glucose sensing (continuous glucose monitoring, cgm) and insulin delivery (continuous subcutaneous insulin infusion, csii) systems, can provide important information for its optimization. in this study, a method for cr optimization based on ...201829355438
polyketides from the deep-sea-derived fungus graphostroma sp. mccc 3a00421 showed potent anti-food allergic activities.to discover anti-food allergic components from deep-sea-derived microorganisms. a systematic chemical investigation was conducted on the atlantic hydrothermal fungus graphostroma sp. mccc 3a00421. consequently, nine new (1-9) and 19 known (10‒28) polyketides were isolated. the planar structures of the new compounds were elucidated mainly by detailed analysis of their nmr and hresims data, while the absolute configurations were established using the modified mosher's method in association with ec ...201829355320
one new indolocarbazole alkaloid from the streptomyces sp. a22.one new indolocarbazole alkaloid, 12-n-methyl-k252c, together with eight known indolocarbazoles were isolated from the rice solid fermentation of the marine-derived streptomyces sp. a22. their structures were elucidated on the basis of spectroscopic methods (uv, ir, hresitof ms, 1d nmr and 2d nmr). all of these compounds were evaluated for bromodomain-containing protein 4 (brd4) inhibitory activities and cytotoxic activity assay, respectively. compounds 4 and 5 showed moderate cytotoxic activity ...201829355042
[ji laixi's experience of acupuncture and moxibustion for knee osteoarthritis].in theory of tcm, the root cause of knee osteoarthritis (xibi) is the deficiency of liver and kidney, while the symptoms are involved with multiple damages of muscles, tendons and joints. the exogenous evil of wind, coldness, dampness and heat as well as the blood stasis usually block the meridians and collaterals, which cause the knee osteoarthritis. professor ji laixi selects acupoints based on syndrome differentiation, and treats both root cause and symptoms. he attaches importance to the ash ...201829354940
[aconite cake-separated moxibustion for knee osteoarthritis with kidney-marrow deficiency].to observe the effects among aconite cake-separated moxibustion, moxibustion and acupuncture for knee osteoarthritis (koa) with kidney-marrow deficiency and to explore the feasibility of cake-separated moxibustion as a home remedy solution.201829354936
[effects of the combined therapy of heat sensitive moxibustion and acupoint injection on endometrial receptivity of hypdrosalphinx infertility in the patients after hysteroscopy and laparoscopy].to explore the effects of the combined therapy of heat sensitive moxibustion and acupoint injection on endometrial receptivity of hypdrosalphinx infertility in the patients after hysteroscopy and laparoscopy on the base of routine post-operative anti-inflammation.201829354932
[moxibustion combined with highly active antiretroviral therapy for cd4+ and γ chain cytokines of hiv infected patients].to compare the effects of moxibustion combined with highly active antiretroviral therapy (haart) and simple haart for human immunodeficiency virus (hiv) infected patients.201829354928
cholinesterase activity in the cup oyster saccostrea sp. exposed to chlorpyrifos, imidacloprid, cadmium and copper.in the present study, the sensitivity and concentration dependence of three functionally-defined components of cholinesterase activity (total: t-che; eserine-sensitive: es-che; and eserine-resistant: er-che) were quantified in the gill, digestive gland and adductor muscle of the tropical cup oyster saccostrea sp., following acute (96h) aqueous exposure to commercial formulations of the organophosphate (op) insecticide chlorpyrifos and the neonicotinoid (nn) imidacloprid (concentration range: 0.1 ...201829353174
molecular monitoring of dihydrofolatereductase (dhfr) and dihydropteroatesynthetase (dhps) associated with sulfadoxine-pyrimethamine resistance in plasmodium vivax isolates of palawan, philippines.the emergence of drug-resistant plasmodium vivax poses problems for malaria control and elimination in some parts of the world, especially in developing countries where individuals are routinely exposed to the infection. the aim of this study was to determine the single nucleotide polymorphisms (snps) in dihydropteroate synthase (pvdhps) and dihydrofolate reductase (pvdhfr) genes associated with sulfadoxine-pyrimethamine (sp) drug resistance among p. vivax isolates collected in palawan, philippi ...201829352991
low socioeconomic condition and the risk of dengue fever: a direct relationship.this study aimed to characterize the first dengue fever epidemic in várzea paulista, são paulo, brazil, and its spatial and spatio-temporal distribution in order to assess the association of socioeconomic factors with dengue occurrence. we used autochthonous dengue cases confirmed in a 2007 epidemic, the first reported in the city, available in the information system on diseases of compulsory declaration database. these cases where geocoded by address. we identified spatial and spatio-temporal c ...201829352990
cost-utility of quadrivalent versus trivalent influenza vaccine in brazil - comparison of outcomes from different static model types.influenza burden in brazil is considerable with 4.2-6.4 million cases in 2008 and influenza-like-illness responsible for 16.9% of hospitalizations. cost-effectiveness of influenza vaccination may be assessed by different types of models, with limitations due to data availability, assumptions, and modelling approach.201829352897
2,3-di-o-sulfo glucuronic acid: an unmodified and unusual residue in a highly sulfated chondroitin sulfate from litopenaeus vannamei.the occurrence of a natural and unmodified highly sulfated chondroitin sulfate from litopenaeus vannamei heads (scs) is herein reported. its partial digestion by chondroitinases ac and abc together with its electrophoretic migration profile revealed it as a highly sulfated chondroitin sulfate despite its average molecular weight being similar to csa. using orthogonal 1d/2d nmr experiments, the anomeric signals (δ 4.62/106.0) corresponding to unusual 2,3-di-o-sulfo-glca (∼36%), u33s (δ 4.42/84.1, ...201829352874
concentration-dependent effect of bleaching agents on the immunolabeling of interleukin-6, interleukin-17, and cd5-positive cells in the dental pulp.to evaluate lymphocyte-like cell activation (cd5-positive cells) and the expression of interleukin (il)-6 and il-17 in the pulp after dental bleaching with two concentrations of hydrogen peroxide (h2 o2 ).201829352770
evaluating new bio-hydrogen producers: clostridium perfringens strain jjc, clostridium bifermentans strain wym and clostridium sp. strain ade.ty.three newly discovered h2 producing bacteria namely clostridium perfringens strain jjc, clostridium bifermentans strain wym and clostridium sp. strain ade.ty originated from landfill leachate sludge have demonstrated highly efficient h2 production. the maximum h2 production attained from these isolates are in the descending order of strain c. perfringens strain jjc > c. bifermentans strain wym > clostridium sp. strain ade.ty with yield of 4.68 ± 0.12, 3.29 ± 0.11, and 2.87 ± 0.10 mol h2/mol gluc ...201829352712
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